Do Tan Phat

Viral diseases caused severe damage for plant growth and development. Penetration and spread of viruses in the host plants dramatically reduce crop yield and quality. Due to the critical damages of viral diseases to agricultural production, different approaches and methods have been developed and utilized to manage, protect and improve plant virus resistance. Of which, conventional methods such as meristem culture, thermotherapy, cryotherapy as well as chemotherapy have been widely used and conducted effective results. Moreover, cross protection and gene pyramiding approaches have performed the broad and stable spectrum resistance potential in different plant species. Recently, advanced methods like plant transformation, gene silencing as well as genome editing have shown great successes in plant virus resistant improvement. In this review, we will briefly introduce the principle, advantages and limitations of different methods used for plant viral diseases management and viral resi...

Hairy root induction system has been widely applied for studies of gene function, gene expression, and genome editing in numerous plant species. In this study, we developed and evaluated the performance of an in vitro hairy root induction system via Agrobacterium rhizogenes on several Vietnamese and international soybean cultivars. The efficacy of in vitro hairy root induction and of transformation using this system was varied and depended on soybean cultivars as well as transgenic constructs. The hairy root induction frequency of different soybean cultivars ranged from 61.67% to 100% after 5 days on culture medium. From 43.8% to 79.8% of hairy roots transformed with GFP-expressing construct showed transgene expression, while that for the construct with the gus gene was from 38.07% to 72.33%. Among tested soybean cultivars, DT26 demonstrated the highest transformation and gene expression efficacy with both investigated vectors. This hairy root induction system was further utilized f...