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Research Interests: Chemistry, Environmental Chemistry, Food Chemistry, Medicine, Multidisciplinary, and 11 moreSolid Phase Extraction, Oryza Sativa, Gas Chromatography, Contamination, Polycyclic aromatic hydrocarbons (PAHs), Seeds, Gas Chromatography/mass Spectrometry, Food Contamination, Phenanthrene, Pyrene, and Quechers
Fusarium graminearum and Fusarium meridionale are primary contaminants of barley, capable of producing several mycotoxins, mainly type B trichothecenes and zearalenone. Cold plasma decontamination has been gaining prominence, seeking to... more
Fusarium graminearum and Fusarium meridionale are primary contaminants of barley, capable of producing several mycotoxins, mainly type B trichothecenes and zearalenone. Cold plasma decontamination has been gaining prominence, seeking to control the fungal and mycotoxin contamination of food and feed and to improve product quality. To reach this objective, the present study was divided into two parts. In the first part, F. meridionale and F. graminearum strains were exposed to gliding arc plasma jet (GAPJ). Cell viability tests showed the inactivation of F. meridionale after 15-min treatment, whereas F. graminearum showed to be resistant. In the second part, barley grains were treated by GAPJ for 10, 20, and 30 min, demonstrating a reduction of about 2 log CFU/g of the barley’s mycobiota, composed of yeasts, strains belonging to the F. graminearum species complex, Alternaria, and Aspergillus. A decrease in DON levels (up to 89%) was observed after exposure for 20 min. However, an inc...
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Deoxynivalenol (DON), one of the main mycotoxins found in food matrices, has high level of toxicity. This study aimed to characterize the peroxidase enzyme extracted from rice bran to be applied to the biodegradation of DON in order to... more
Deoxynivalenol (DON), one of the main mycotoxins found in food matrices, has high level of toxicity. This study aimed to characterize the peroxidase enzyme extracted from rice bran to be applied to the biodegradation of DON in order to evaluate the potential peroxidase (PO) from rice bran (RB) has to degrade DON in optimal conditions. Purification and recovery factors of PO extracted from RB and purified by three-phase partitioning were 5.7% and 50%, respectively. PO had the highest level of activity in the phosphate buffer 5 mM pH 5.5 in both crude and purified forms, whose reaction temperatures were 25°C and 10°C. At the end of production, purification and characterization steps, specific activities of the bran were 115.79 U mg and 4363 U g. Reduction in the mycotoxin DON in optimal conditions determined for PO from RB was 20.3%, a promising result when the aim is to adequate mycotoxicological levels to foods.
Research Interests: Chemistry, Food Science, Medicine, Peroxidase, Pesticide, and 9 moreTemperature, Enzyme, Public health systems and services research, Mycotoxin, Food Contamination, Hydrogen-Ion Concentration, Journal of Environmental Health Science and Engineering, ENVIRONMENTAL SCIENCE AND MANAGEMENT, and trichothecenes
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The antifungal and antimycotoxigenic activities of extracts from edible plants were tested by the agar dilution method using the growth diameter of Aspergillus flavus as response and the determination of aflatoxins B1 and B2 in the... more
The antifungal and antimycotoxigenic activities of extracts from edible plants were tested by the agar dilution method using the growth diameter of Aspergillus flavus as response and the determination of aflatoxins B1 and B2 in the culture medium. On the 7th incubation day, the greatest fungal inhibitions were reached by the extracts from potato peel; rice and wheat; lemon peel and pulp; eggplant peel; orange peel and pulp; and apple pulp. After the 14-day incubation, the extracts from banana (30 µg phenol/ml agar), eggplant (30 µg phenol/ml agar), and potato (50 and 67 µg phenol/ml agar) pulp reduced the production of aflatoxin B1 by 3.2%/µg phenol/ml agar, 2.9%/µg phenol/ml agar, 1.8%/µg phenol/ml agar and 0.85%/µg phenol/ml agar, respectively, in relation to the control. The extracts from the other vegetables fully inhibited the synthesis of the mycotoxin. These results point to the studied plants and their residues as potential sources of phenolic compounds that may have an inhi...
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The aim of this study was to investigate the use of protein-phenolic based coating made from fermented rice bran on cherry tomatoes (Lycopersicum esculentum). Tests were performed with glycerol 3% (v/v), glycerol with protein-phenolic... more
The aim of this study was to investigate the use of protein-phenolic based coating made from fermented rice bran on cherry tomatoes (Lycopersicum esculentum). Tests were performed with glycerol 3% (v/v), glycerol with protein-phenolic rice bran extract (5%), glycerol with protein-phenolic extract after 96 hours of fermentation (5%), and a control (without coating). The coated cherry tomatoes were kept at room temperature for 28 days. Mass loss, pH and acidity, total soluble solids, and carotenoids were determined every 96 hours. The coating made from the biomass extract reduced the carotenoid and acidity levels in the fruits studied by 17 and 21.1%, respectively, compared to the control. The coating proved an efficient barrier to water vapor with mass loss of 57% less than the control suggesting that it can be used as an alternative for vegetable tissue conservation.
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Research Interests: Engineering, Chemistry, Chromatography, Pesticide Residues, Medicine, and 11 moreSample Preparation, Oryza Sativa, Tandem Mass Spectrometry, High Pressure Liquid Chromatography, CHEMICAL SCIENCES, Agricultural and Food Chemistry, Sensitivity and Specificity, Soil Test, Azoxystrobin, Soil Pollutants, and Quechers
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The main aim of this work was to investigate the effects of temperature and nitrogen concentration on the antioxidant potential of extracts from Spirulina (Arthospira) platensis biomass. S. platensis biomass obtained at 35ºC and with... more
The main aim of this work was to investigate the effects of temperature and nitrogen concentration on the antioxidant potential of extracts from Spirulina (Arthospira) platensis biomass. S. platensis biomass obtained at 35ºC and with 1.875 g.L-1 or 2.5 g.L-1 of sodium nitrate in the culture medium presented higher concentrations of phenolic compounds. The antioxidant potential of methanol extracts of biomass on the enzymatic browning caused by peroxidase were 29 and 35%, respectively, being the reduction related to the amount of phenolic compounds present in this extract.
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... Biochem J 2007; 401 (1): 1–11. [ Links ]. 9. Shah SV, Baliga R, Rajapurkar M, Fonseca VA. Oxidants in chronic kidney disease. ... Food Chem 2009; 115 (3): 801-5. [ Links ]. 11. Devi MKA, Gondi M, Sakthivelu G, Giridhar P, Rajasekaran... more
... Biochem J 2007; 401 (1): 1–11. [ Links ]. 9. Shah SV, Baliga R, Rajapurkar M, Fonseca VA. Oxidants in chronic kidney disease. ... Food Chem 2009; 115 (3): 801-5. [ Links ]. 11. Devi MKA, Gondi M, Sakthivelu G, Giridhar P, Rajasekaran T, Ravishankar GA. ...
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Research Interests: Engineering, Chemistry, Fungi, Medicine, Mycotoxins, and 13 moreOryza Sativa, Tandem Mass Spectrometry, Aflatoxin, High Pressure Liquid Chromatography, CHEMICAL SCIENCES, Mycotoxin, Food Contamination, Agricultural and Food Chemistry, Agricultural Irrigation, Zearalenone, Ochratoxin A, food handling, and Fungicide
Abstract Trichothecenes are a group of mycotoxins found in wheat infected by Fusarium fungi during cultivation. Wheat flour is one of the main vehicles for mycotoxin introduction into the industrial environment, and is raw material of... more
Abstract Trichothecenes are a group of mycotoxins found in wheat infected by Fusarium fungi during cultivation. Wheat flour is one of the main vehicles for mycotoxin introduction into the industrial environment, and is raw material of various bakery products, such as pasta, cakes, breads and biscuits. The objective of this study was to evaluate the occurrence of DON, HT-2 and T-2 toxins in Cracker biscuits produced in southern Brazil. For this purpose, the method for simultaneous determination of trichothecenes DON, HT-2 and T-2 toxins was standardized. The determination of trichothecenes A and B was carried out by HPLC-DAD, using ACN: H 2 O (96:4, v/v) as mobile phase. The recoveries of DON, HT-2 and T-2 toxins in biscuits were 99%, 84%, and 81%, whose matrix effect increased the signal by 14%, 3%, and 10%, respectively. The study showed that 18 of 23 samples were contaminated with DON (378–5295 μg kg −1 ); of which 22% were above the maximum limit established by Brazilian law. The T-2 and HT-2 toxins were not detected in the samples. Considering the contamination the maximum DON level that can be ingested through the consumption of Cracker biscuits is 0.08 μg kg −1 body weight per day. These results demonstrate the importance in establishing the actual consumption of wheat based products in Brazil, since these products are widely consumed by different age groups of the population.
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The main aim of this work was to investigate the effects of temperature and nitrogen concentration on the antioxidant potential of extracts from Spirulina (Arthospira) platensis biomass. S. platensis biomass obtained at 35ºC and with... more
The main aim of this work was to investigate the effects of temperature and nitrogen concentration on the antioxidant potential of extracts from Spirulina (Arthospira) platensis biomass. S. platensis biomass obtained at 35ºC and with 1.875 g.L-1 or 2.5 g.L-1 of sodium nitrate in the culture medium presented higher concentrations of phenolic compounds. The antioxidant potential of methanol extracts of biomass on the enzymatic browning caused by peroxidase were 29 and 35%, respectively, being the reduction related to the amount of phenolic compounds present in this extract.