The application of a newly developed HPTLC-bioautography assay for detecting peroxidase enzyme inhibitors in plant extracts in addition to bioautography methods for detecting antioxidant compounds resulted in the isolation of a new... more
The application of a newly developed HPTLC-bioautography assay for detecting peroxidase enzyme inhibitors in plant extracts in addition to bioautography methods for detecting antioxidant compounds resulted in the isolation of a new biflavonoid 3'-methoxy sahranflavone along with two known biflavonoids and three flavonoids from the leaves and cones of Juniperus communis, J. horizontalis and J. chinensis. The structures of all compounds were elucidated by means of 1 D and 2 D NMR and MALDI-TOF MS technique in addition to comparison to literature data. Quantitative estimation of antiperoxidase and antioxidative capacity based on DPPH free radical scavenging activity and β-carotene bleaching of extracts, active fraction and constituents was achieved by applying validated high resolution image analyses techniques. 3'-methoxy sahranflavone and quercetrin possessed high mutual antiperoxidase and antioxidant activities. Molecular docking simulations were performed to reveal the interaction of isolated compounds with human myeloperoxidase enzyme on the molecular level indicating the potential anti-inflammatory activity of 3'-methoxy sahranflavone and quercetrin.
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This study compares the chloroform extracts of bulbs and roots of Narcissus papyraceus Ker Gawl. and Narcissus tazetta L. The cytotoxicity of the plant extracts was evaluated against human hepatocellular carcinoma cell line (HEPG2) and... more
This study compares the chloroform extracts of bulbs and roots of Narcissus papyraceus Ker Gawl. and Narcissus tazetta L. The cytotoxicity of the plant extracts was evaluated against human hepatocellular carcinoma cell line (HEPG2) and colon carcinoma cell line (HCT116) in comparison to doxorubicin. The extracts from the after-flowering (AF) bulbs of N. tazetta L. and N. papyraceus exhibited strong cytotoxic activity against HEPG2 (IC50: 2.2, 3.5 μg mL(-1)) and HCT116 (IC50: 4.2, 3.9 μg mL(-1)) cell lines, respectively. N. tazetta L. bulbs exhibited the least cell viability percentage in HepG-2 cell line (5.32%), while the AF root extracts of N. papyraceus exhibited the least cell viability percentage in HCT116 cell line (4.93%), when applied at a concentration of 50 μg mL(-1), thereby being more active than doxorubicin at the same concentration.
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The work reported in this paper aims at developing an accurate, specific, repeatable and robust HPTLC method for the determination of galanthamine in different Amaryllidaceae plant extracts.
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ABSTRACT A rapid, accurate, specific, repeatable and robust HPTLC method for the determination of lycorine in different Amaryllidaceae plant extracts is presented in this work. No article related to the HPTLC determination of lycorine in... more
ABSTRACT A rapid, accurate, specific, repeatable and robust HPTLC method for the determination of lycorine in different Amaryllidaceae plant extracts is presented in this work. No article related to the HPTLC determination of lycorine in plant extracts has been reported in literature. Lycorine, a common alkaloid of family Amaryllidaceae, moreover, there have been some recent reports which reveal the interaction of lycorine with DNA and tRNA. It has, therefore, been to the interest of phytochemists to determine the content of this alkaloid in Amaryllidaceaous plants.
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A phytochemical investigation of Spergularia marina (L.) Griseb. growing in Egypt, has been carried out, which resulted in the isolation of seven compounds from the different extracts of the plant namely; β- sitosterol glucoside, tricin... more
A phytochemical investigation of Spergularia marina (L.) Griseb. growing in Egypt, has been carried
out, which resulted in the isolation of seven compounds from the different extracts of the plant namely; β-
sitosterol glucoside, tricin (1) dihydroferulic acid (2), vanillic acid (3), 4-hydroxybenzoic acid (4), uracil (5) and 8-
hydroxy cuminoic acid (6) Structure elucidation of the isolated compounds was carried out using different
spectroscopic techniques. This is the first report for the isolation of these compounds from genus Spergularia.
Furthermore, 8-Hydroxy cuminoic acid and uracil were isolated for the first time from family Caryophyllaceae.
The chemical composition of the volatile components present in the petroleum ether extract of Spergularia
marina (L.) Griseb. using combined gas chromatography-mass spectrometry (GC-MS) is reported here for the
first time. Of the 97 components present, 59 were identified including three sulfur containing compounds which
represented about 1.8% of the volatiles of the total petroleum ether extract. This prompted us to study and report
its possible antimicrobial activity. In addition, the antibacterial and antifungal screening of different extracts of
Spergularia marina (L.) Griseb. as well as some isolates have been performed using agar diffusion method.
out, which resulted in the isolation of seven compounds from the different extracts of the plant namely; β-
sitosterol glucoside, tricin (1) dihydroferulic acid (2), vanillic acid (3), 4-hydroxybenzoic acid (4), uracil (5) and 8-
hydroxy cuminoic acid (6) Structure elucidation of the isolated compounds was carried out using different
spectroscopic techniques. This is the first report for the isolation of these compounds from genus Spergularia.
Furthermore, 8-Hydroxy cuminoic acid and uracil were isolated for the first time from family Caryophyllaceae.
The chemical composition of the volatile components present in the petroleum ether extract of Spergularia
marina (L.) Griseb. using combined gas chromatography-mass spectrometry (GC-MS) is reported here for the
first time. Of the 97 components present, 59 were identified including three sulfur containing compounds which
represented about 1.8% of the volatiles of the total petroleum ether extract. This prompted us to study and report
its possible antimicrobial activity. In addition, the antibacterial and antifungal screening of different extracts of
Spergularia marina (L.) Griseb. as well as some isolates have been performed using agar diffusion method.