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bitstream/item/30063/1/Caracterizacao-distribuicao.pd
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Insect viruses have been used to protect crops and forests worldwide for decades. Among insect viruses, isolates of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) have proven potential for the control of Spodoptera... more
Insect viruses have been used to protect crops and forests worldwide for decades. Among insect viruses, isolates of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) have proven potential for the control of Spodoptera frugiperda (J. E. Smith) (FAW) (Lepidoptera: Noctuidae), a pest of many economically essential crops across several continents. Mass production of SfMNPV depends on an in vivo system using host insect rearing. However, many factors can limit its production, including abiotic factors and host characteristics, such as the stage of development and an antagonist intraspecific interaction. Thus, to improve in vivo production, we verified the most suitable larval age to inoculate the virus and the influence of incubation temperature on viral production. Subsequently, cannibal behavior was verified in FAW larvae reared at different densities, while reproducing the conditions of the best treatments. The highest viral yield occurred when FAW larvae were inoculated at 10 and 8 days old and incubated at 22 °C and 25 °C, respectively. Nonetheless, survival (lethal period in days) and cannibal behavior were positively influenced by larval development, which potentially increases the load of contamination and requires larval individualization for these production conditions. In contrast, 4-day-old larvae, which were inoculated and incubated at 31 °C, also demonstrated high viral production, with lower rates of cannibalism and death on the same day, thereby showing potential. The information presented in this study is useful for the optimization of the in vivo production systems of SfMNPV.
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Research Interests: Biology and Hymenoptera
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Research Interests: Zoology, Biology, Noctuidae, Larva, Helicoverpa, and 3 moreHelicoverpa armigera, HELICOVERPA-ZEA, and Pupa
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Research Interests: Zoology, Biology, Biological Control, Medicine, Virulence, and 14 moreAnimals, Invertebrate Pathology, Noctuidae, Biopesticide, Larva, Chitinase, Amino Acid Sequence, Base Sequence, SISTEM INTEGUMENT, Spodoptera litura, Molecular Sequence Data, frameshift mutation, biological control agents, and Nucleopolyhedrovirus
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A utilização de plantas geneticamente modificadas e biopesticidas, constituem importantes ferramentas no controle de pragas na cultura do milho. As formigascortadeiras, apesar de não serem pragas do milho, ocorrem nesses agroecossistemas... more
A utilização de plantas geneticamente modificadas e biopesticidas, constituem importantes ferramentas no controle de pragas na cultura do milho. As formigascortadeiras, apesar de não serem pragas do milho, ocorrem nesses agroecossistemas e causam níveis reduzidos de desfolha. Objetivou-se avaliar a preferência de corte e área foliar cortada por Atta sexdens entre milho Bt (30A91 HX e AG7088 PRO-II) pulverizados com cepas de Bt e os respectivos isogênicos. Em laboratório (22 ±2oC, 73±3%, 12 horas de fotoperíodo) foram oferecidas uma vez ao dia plantas de milho (45 cm altura) Bt expressando as proteínas CryIF e CryIA 105 (CryIAb, CryIAc, CryIF) e, os isogênicos não Bt à quatro colônias de A. sexdens. Efetuaram-se seis bioensaios por colônia, onde a atividade das formigas foi avaliada por um período de 30 minutos. A cada teste, as posições das plantas foram alternadas para evitar o condicionamento das formigas. Foram avaliados o tempo gasto para o início do corte das plantas, a porcent...
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The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae),... more
The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTITn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDSPage of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. T...
Research Interests: Zoology, Biology, Medicine, Hybrid, Noctuidae, and 4 morePEST analysis, Larva, Fall armyworm, and Spodoptera litura
Aims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against... more
Aims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that
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The cultivation of genetically modified (GM) crops has raised numerous concerns in the European Union and other parts of the world about their environmental and economic impact. Especially outcrossing of genetically modified organisms... more
The cultivation of genetically modified (GM) crops has raised numerous concerns in the European Union and other parts of the world about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dispersal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GM maize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semi-urban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GM pollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this 'GM pollen monitoring by bioaerosol sampling and PCR screening' approach might represent an useful aid in the surveillance of GM-free areas, centres of origin and natural reserves.
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Two nonoverlapping autosomal inversions defined unusual neo-sex chromosomes in the Hessian fly (Mayetiola destructor). Like other neo-sex chromosomes, these were normally heterozygous, present only in one sex, and suppressed recombination... more
Two nonoverlapping autosomal inversions defined unusual neo-sex chromosomes in the Hessian fly (Mayetiola destructor). Like other neo-sex chromosomes, these were normally heterozygous, present only in one sex, and suppressed recombination around a sex-determining master switch. Their unusual properties originated from the anomalous Hessian fly sex determination system in which postzygotic chromosome elimination is used to establish the sex-determining karyotypes. This system permitted the evolution of a master switch (Chromosome maintenance, Cm) that acts maternally. All of the offspring of females that carry Cm-associated neo-sex chromosomes attain a female-determining somatic karyotype and develop as females. Thus, the chromosomes act as maternal effect neo-W's, or W-prime (W′) chromosomes, where ZW′ females mate with ZZ males to engender female-producing (ZW′) and male-producing (ZZ) females in equal numbers. Genetic mapping and physical mapping identified the inversions. The...
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Eventos de milho geneticamente modificado (GM) expressando proteínas inseticidas de Bacillus thuringiensis (Bt) têm sido cultivados comercialmente no Brasil desde 2008. O monitoramento dessas plantas transgênicas após a liberação... more
Eventos de milho geneticamente modificado (GM) expressando proteínas inseticidas de Bacillus thuringiensis (Bt) têm sido cultivados comercialmente no Brasil desde 2008. O monitoramento dessas plantas transgênicas após a liberação comercial deve ser feito a fim de analisar e avaliar os possíveis efeitos ambientais sobre organismos não alvo. Microrganismos no solo podem entrar em contato com as proteínas Bt quando estas são liberadas em exsudatos de raízes de milho Bt ou tecidos da planta em decomposição, sendo que a influência dessas proteínas sobre a composição da microbiota ainda é pouco compreendida. O objetivo deste estudo foi avaliar o impacto de eventos comerciais de milho transgênico expressando proteínas Bt na comunidade bacteriana do solo rizosférico. Para este fim, os milhos transgênicos MON 810, Bt 11, Herculex, MON 89034, VTPro II e Viptera, seus respectivos isogênicos tratados e não tratados com inseticidas químicos foram avaliados utilizando placas de Biolog EcoPlates? ...
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bitstream/item/149807/1/Monitoramento-Spodoptera-frugiperda.pd
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Por causa da grande importância dos recursos microbianos, em 2012, foi instituida a Colecao de Referencia da Embrapa Milho e Sorgo, junto a Rede de Recursos Geneticos Microbianos da Embrapa. O foco principal desta colecao e a prospeccao... more
Por causa da grande importância dos recursos microbianos, em 2012, foi instituida a Colecao de Referencia da Embrapa Milho e Sorgo, junto a Rede de Recursos Geneticos Microbianos da Embrapa. O foco principal desta colecao e a prospeccao da biodiversidade, com a manutencao de seu acervo e organizacao da informacao, garantindo a conservacao dos recursos biologicos nacionais, visando sua aplicacao em pesquisa, no agronegocio e em outros setores produtivos relacionados. Este “Manual de Gestao da Colecao de Microrganismos Multifuncionais e Fitopatogenicos (CMMF)” descreve o sistema de gestao implementado e documentado pela CMMF, o sistema de qualidade e seguranca, os processos operacionais e tecnicos da manutencao e preservacao dos acessos pertencentes ao banco de germoplasma microbiano e a estrutura organizacional e da documentacao.