Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bact... more Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry-intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.
Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immob... more Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or "hetero"-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10-12 M to 10-1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interacti...
Perioperative hyperglycaemia is associated with poor outcomes in patients undergoing cardiac surg... more Perioperative hyperglycaemia is associated with poor outcomes in patients undergoing cardiac surgery. Frequent postoperative hyperglycaemia in cardiac surgery patients has led to the initiation of an insulin infusion sliding scale for quality improvement. A systematic review was conducted to determine whether a protocol-directed insulin infusion sliding scale is as safe and effective as a conventional practitioner-directed insulin infusion sliding scale, within target blood glucose ranges. A literature survey was conducted to identify reports on the effectiveness and safety of an insulin infusion protocol, using seven electronic databases from 2000 to 2012: MEDLINE, CINAHL, EMBASE, the Cochrane Library, the Joanna Briggs Institute Library and SIGLE. Data were extracted using pre-determined systematic review and meta-analysis criteria. Seven research studies met the inclusion criteria. There was an improvement in overall glycaemic control in five of these studies. The implementation ...
The Food and Agricultural Policy Research Institute (FAPRI) received a request in mid-February to... more The Food and Agricultural Policy Research Institute (FAPRI) received a request in mid-February to analyze the proposed changes to agriculture and agricultural trade made by Arthur Dunkel. These changes essentially fall into three areas. 1) Export Competition. Subsidies are subject to reduction in two ways. Expenditures are to be reduced by 36 percent and quantities exported with the benefit of
ABSTRACT The FAPRI 2002 World Agricultural Outlook presents final projections of FAPRI's ... more ABSTRACT The FAPRI 2002 World Agricultural Outlook presents final projections of FAPRI's agricultural outlook on world agricultural production, consumption, and trade.
Critical Reviews in Food Science and Nutrition, 2014
In the contemporary society, diabetes mellitus is considered as a common, growing, serious, costl... more In the contemporary society, diabetes mellitus is considered as a common, growing, serious, costly, and potentially preventable public health problem. It is forecasted that in 2030, the number of people with diabetes will go up from 117 million in 2000 to 366 million in 2030. The prevalence of diabetes will place a huge burden on health and financial structures of countries, and these will impact on individuals, as well as families and nations. Polysaccharides, para-aminobenzoic acid, fixed oils, sterol, proteins, and peptides are biologically active ingredients, which are found in pumpkins. The chemicals within pumpkins such as the fruit pulp, oil from ungerminated seeds, and protein from germinated seeds have hypoglycemic properties. Preliminary investigation showed that pumpkin seeds, and the macromolecules, therein, such as Trigonelline (TRG), Nicotinic acid (NA), and D-chiro-inositol (DCI), possess hypoglycemic properties and could assist in maintaining glycemic control.
Impacts of WTO restrictions on subsidized EU sugar exports ... Daneswar Poonyth, Patrick Westhof... more Impacts of WTO restrictions on subsidized EU sugar exports ... Daneswar Poonyth, Patrick Westhoffb9*, Abner Womackb, Gary Adams a Department of Agricultural Economics, Extension and Rural Development, University of Pretoria, Pretoria, South Africa Food ...
Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bact... more Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry-intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.
Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immob... more Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or "hetero"-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10-12 M to 10-1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interacti...
Perioperative hyperglycaemia is associated with poor outcomes in patients undergoing cardiac surg... more Perioperative hyperglycaemia is associated with poor outcomes in patients undergoing cardiac surgery. Frequent postoperative hyperglycaemia in cardiac surgery patients has led to the initiation of an insulin infusion sliding scale for quality improvement. A systematic review was conducted to determine whether a protocol-directed insulin infusion sliding scale is as safe and effective as a conventional practitioner-directed insulin infusion sliding scale, within target blood glucose ranges. A literature survey was conducted to identify reports on the effectiveness and safety of an insulin infusion protocol, using seven electronic databases from 2000 to 2012: MEDLINE, CINAHL, EMBASE, the Cochrane Library, the Joanna Briggs Institute Library and SIGLE. Data were extracted using pre-determined systematic review and meta-analysis criteria. Seven research studies met the inclusion criteria. There was an improvement in overall glycaemic control in five of these studies. The implementation ...
The Food and Agricultural Policy Research Institute (FAPRI) received a request in mid-February to... more The Food and Agricultural Policy Research Institute (FAPRI) received a request in mid-February to analyze the proposed changes to agriculture and agricultural trade made by Arthur Dunkel. These changes essentially fall into three areas. 1) Export Competition. Subsidies are subject to reduction in two ways. Expenditures are to be reduced by 36 percent and quantities exported with the benefit of
ABSTRACT The FAPRI 2002 World Agricultural Outlook presents final projections of FAPRI's ... more ABSTRACT The FAPRI 2002 World Agricultural Outlook presents final projections of FAPRI's agricultural outlook on world agricultural production, consumption, and trade.
Critical Reviews in Food Science and Nutrition, 2014
In the contemporary society, diabetes mellitus is considered as a common, growing, serious, costl... more In the contemporary society, diabetes mellitus is considered as a common, growing, serious, costly, and potentially preventable public health problem. It is forecasted that in 2030, the number of people with diabetes will go up from 117 million in 2000 to 366 million in 2030. The prevalence of diabetes will place a huge burden on health and financial structures of countries, and these will impact on individuals, as well as families and nations. Polysaccharides, para-aminobenzoic acid, fixed oils, sterol, proteins, and peptides are biologically active ingredients, which are found in pumpkins. The chemicals within pumpkins such as the fruit pulp, oil from ungerminated seeds, and protein from germinated seeds have hypoglycemic properties. Preliminary investigation showed that pumpkin seeds, and the macromolecules, therein, such as Trigonelline (TRG), Nicotinic acid (NA), and D-chiro-inositol (DCI), possess hypoglycemic properties and could assist in maintaining glycemic control.
Impacts of WTO restrictions on subsidized EU sugar exports ... Daneswar Poonyth, Patrick Westhof... more Impacts of WTO restrictions on subsidized EU sugar exports ... Daneswar Poonyth, Patrick Westhoffb9*, Abner Womackb, Gary Adams a Department of Agricultural Economics, Extension and Rural Development, University of Pretoria, Pretoria, South Africa Food ...
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