Hamideh Roshanfekr

Scientists are currently working to develop more effective and less harmful methods of delivering drugs to tissue. One method is to use a special type of carrier to help the drug get to the right place in the body. In this study, an organometallic framework nanocarrier with the formula IRMOF-3 [Zn4O(NH2-BDC)3] was successfully prepared, containing magnetic nanoparticles of manganese ferrite (MnFe2O4) and the drug doxorubicin encapsulated in a pH- and temperature-sensitive smart polymer of poly-N-isopropylacrylamide. Doxorubicin, an anticancer drug, was loaded into the pores of a magnetic organometallic framework and modified with the smart polymer poly(N-isopropylacrylamide-co-AA), which has a lower critical solution temperature (LCST) of less than 38 °C. The synthesis of magnetic nanoparticles and magnetic organometallic frameworks encapsulated in smart polymer was investigated using various analytical techniques such as Fourier transform infrared spectrometer, thermal stability an...

ABSTRACT Hybridomas secreting monoclonal antibodies (MAbs) producing stable, specific and high affinity against human serum albumin (HSA) have been established. The aim of the present study was the production of MAbs that will be potentially used in designing immunoassay methods especially immunochromatography assay kit for screening of microalbuminuria (MAU) in the early detection of diabetic and nondiabetic nephropathy. The hybridomas were obtained by fusion of spleen cells from immunized mice with mouse myeloma cell line (SP2). After limiting dilutions three clones producing antibodies were designed as EMRC1-3, which displayed different pattern of fine specificity for HSA and low cross reaction with other proteins as elucidated by inhibition enzyme-linked immunosorbent assay (ELISA). These clones were found to be of immunoglobulin G (IgG) class with k light chain. Subclass determination showed that all three MAbs secreted IgG1 type of antibody. The results of affinity purification for the two selected clones (EMRC1 and EMRC3) displayed high affinity with no cross reactivity with any of the related protein molecules. The stable hybridomas secreting anti-HSA were expanded in 50-mL flasks for large-scale production of the required antibodies. The standard curves were constructed with a sensitivity of 10 pg per well covering up to 100 ng per well. The high binding activity to HSA antigen and having no cross reactivity with other related molecules illustrated the potential application of these antibodies as an immunodiagnostic reagent in designing an immunochromatography assay kit for screening of MAU in diabetic and nondiabetic patients.

In this study, two efficient applications for a novel modified polymer (polyethylene terephthalate [PET] modified with AgCl nanoparticles) were proposed and experimentally evaluated. First, PET-AgCl NPs were applied as an adsorbent to remove methylene blue (MB) dye, and the effect of pH, incubation time, concentration of MB, and temperature on the dye removal were studied and optimized to improve dye removal efficiency. The optimum condition included pH 9, temperature 45 ⁰C, and incubation time 24 h. The adsorption fitted the Temkin isotherm model. In the other part of the study, a composite of PET-AgCl NPs with multiwall carbon nanotubes (MWCNTs) was used to modify the gold electrode in order to detect MB dye.  The modified electrode exhibited a linear detection range, 5 μM to 10 nM, with a detection limit of 4.6 nM. DOR: https://dorl.net/dor/20.1001.1.26457776.2021.5.2.2.8

In this study, the nanofiltration (NF) membrane filled with magnetically modified metal organic framework nanoparticle (MOF@Fe3O4) was fabricated by employing the conventional phase inversion method. The synthesized nanoparticle was identified by FTIR, XRD, TEM and BET techniques. The MOF@Fe3O4 incorporated and bare NF membranes were characterized by analysis of EDX, EDS, SEM, TGA, water contact angle measurement, porosity and mean pore size. The performance of the fabricated membranes was evaluated in terms of permeability and antifouling activities. Further, in order to examine dye rejection of the chosen membrane, acid orange 7 was employed as a model pollutant. The effect of different variables, i.e., pH and temperature at three different levels, on the membrane function while filtering dye solution was studied by employing central composite design (CCD) of the response surface methodology (RSM) using Design Expert Software. Thermal stability of the modified membranes was enhanced after the introduction of MOF@Fe3O4. According to experimental findings, 0.5 wt.% incorporated NF membrane revealed the best performance. The highest dye removal efficiency was obtained at a pH and temperature of 3 and 25 °C, respectively.

In this study, a PVC membrane electrode was modified by a derivative of lawason that is a neutral ionophore for Cd (II) determination in alloy and wastewater as real samples. The modified electrode showed a linear electrochemical response to Cd (II) concentration between 5.0×10−8 to 1.5×10−1 molL-1 (LOD = 2.51×10−8 molL-1). In selecting and determining the level of an effective composite of the membrane, the highest results were achieved in the membrane composition 33% PVC, 64.0% TEHP, 1.5% NaTPB, and 1.5% ionophore. The time it takes for the response to reach its maximum and remain stable at this electrode is very favorable (response time < 12 s). The responses of the electrode in consecutive uses for two months did not deviate significantly. In investigating the disturbance of other ions and compounds in the measurement of cadmium, the designed electrode had a very good selectivity for cadmium. The best conditions for measuring cadmium with this electrode are in an acidic envir...

Erythrosine B (ErB) is a xanthenes approved dye that widely used in various fields such as foods, drugs and cosmetics. This work intended to analyze the interaction of this dye on synthesized graphene quantum dot conjugated cysteamine functionalized gold nanoparticles. Pyrolysis of citric acid was applied to graphene quantum dots (GQDs) synthesis. These are zero dimensional materials promising considerable applications because of their extraordinary physicochemical properties. In this work, to improve its applications conjugates of GQDs and gold nanoparticles (GQD-AuNPs) are synthesized and characterized with FESEM, TEM, EDX and FTIR techniques. Interaction of ErB with synthesized conjugates was investigated using fluorescence technique and the results demonstrate that ErB can quench fluorescence of GQD-AuNPs conjugates, considerably. At last, we used of this good interaction for construction sensor for detection of ErB in the concentration range of 1.2 nM to 50 nM and the obtained LOD was 0.03 nM (S/N = 3) with correlation coefficient of (99%).

Abstract In this work, we developed an ultrasensitive and ultraselective electrochemical biosensor for detection of a specific sequence DNA. It was fabricated based on a glassy carbon electrode (GCE) modified with Multi-walled carbon nanotubes (MWCNTs)-Chitosan (CHIT) nanocomposite and a thin film of gold nanoparticles (AuNPs). Immobilization is based on the covalent reaction between thiol group in DNA probe and AuNPs on electrode surface. Cyclic Voltametry (CV) and Differential Pulse Voltammetry (DPV) techniques were used for hybridization detection by methylene blue (MB) as indicator. The surface modification increased significantly DNA immobilization quantity and complementary DNA detection sensitivity. The response signal increased linearly with the increase of the target DNA concentration in the range of (1 × 10-14 M to 9 × 10-14 M) with the detection limit of 7.2 fM (LOD = 3 SX m ). The presented method exhibited excellent specificity and selectivity for complementary and single-mismatch, three-base-mismatch and non-complementary sequences of DNA.

Fluorescence spectroscopy, ultraviolet-visible absorption spectroscopy, circular dichroism spectroscopy, viscometry, cyclic voltammetry, and differential pulse voltammetry were applied to investigate the competitive interaction of DNA with the three new cycloalkyl α-aminobisphosphonates (D1-D3) and spectroscopic probe, neutral red dye, and Hoechst (HO), in a Tris-hydrogen chloride buffer (pH 7.4). The spectroscopic and voltammetric studies showed that the groove binding mode of interaction is predominant in the solution containing DNA and α-aminobisphosphonates. Furthermore, the results indicated that α-aminobisphosphonate with the lengthy N alkyl chains and larger heterocyclic ring size had a stronger interaction. The principal component analysis and theoretical quantum mechanical and molecular mechanics (QM-DFT B3LYP/6-31+G* and MM-SYBYL) methods were also applied to determine the number of chemical components presented in complexation equilibrium and identify the structure comple...

Hybridomas secreting monoclonal antibodies (MAbs) producing stable, specific and high affinity against human serum albumin (HSA) have been established. The aim of the present study was the production of MAbs that will be potentially used in designing immunoassay methods especially immunochromatography assay kit for screening of microalbuminuria (MAU) in the early detection of diabetic and nondiabetic nephropathy. The hybridomas were obtained by fusion of spleen cells from immunized mice with mouse myeloma cell line (SP2). After limiting dilutions three clones producing antibodies were designed as EMRC1-3, which displayed different pattern of fine specificity for HSA and low cross reaction with other proteins as elucidated by inhibition enzyme-linked immunosorbent assay (ELISA). These clones were found to be of immunoglobulin G (IgG) class with k light chain. Subclass determination showed that all three MAbs secreted IgG1 type of antibody. The results of affinity purification for the...

The interaction of native calf thymus DNA (CT-DNA) with sesamol (3,4-methylenedioxyphenol) in Tris-HCl buffer at neutral pH 7.4 was monitored by absorption spectrophotometry, viscometry and spectrofluorometry. It is found that sesamol molecules could interact with DNA outside and/or groove binding modes, as are evidenced by: hyperchromism in UV absorption band, very slow decrease in specific viscosity of DNA, and small increase in the fluorescence of methylene blue (MB)-DNA solutions in the presence of increasing amounts of sesamol, which indicates that it is able to partially release the bound MB. Furthermore, the enthalpy and entropy of the reaction between sesamol and CT-DNA showed that the reaction is enthalpy-favored and entropy-disfavored (ΔH = -174.08 kJ mol(-1); ΔS = -532.92 J mol(-1) K(-1)). The binding constant was determined using absorption measurement and found to be 2.7 × 10(4) M(-1); its magnitude suggests that sesamol interacts to DNA with a high affinity.

The DNA binding behavior of [Cu(4,7-dmp)(phen-dione)Cl]Cl (1) and [Cu(2,9-dmp)(phen-dione)Cl]Cl (2) where dmp and phen-dion stand for dimethyl-1,10-phenanthroline and 1,10-phenanthroline-5,6-dion, respectively, was studied with a series of techniques including Viscometry, UV-Vis absorption, circular dichroism and fluorescence spectroscopy. Cytotoxicity effect was also investigated. Thermodynamic parameters, enthalpy and entropy changes were calculated according to Van't Hoff equation, which indicated that both reactions are predominantly enthalpically driven. However, these two complexes show different behavior in fluorescence, circular dichroism and viscometry methods which indicate the Cu(II) complexes interact with calf-thymus DNA by different mode of binding. These have further been verified by competition studies using Hoechst as a distinct groove binder. All these results indicate that these two complexes (1) and (2) interact with CT-DNA via groove binding and partially intercalative mode, respectively and the binding affinity of the complex 1 is higher than that of complex 2. Finally, our findings suggest that the type of ligands and structure of complexes have marked effect on the binding affinity of complexes involving CT-DNA. Also, these new complexes showed excellent antitumor activity against human T lymphocyte carcinoma-Jurkat cell line.

3, 5, 6-Trichloro-2-pyridinol (TCP) is a stable metabolite of two major pesticides, Chlopyrifos insecticide and Triclopyr herbicide, which are widely used in the world. The potential health hazard associated with TCP is identified due to its high affinity to the DNA molecule. Therefore, in this study, the interaction of native calf thymus DNA with TCP has been investigated using spectrophotometric, circular dichroism (CD), spectrofluorometric, viscometric and voltametric techniques. It was found that TCP molecules could interact with DNA via a groove-binding mode, as evidenced by hyperchromism, with no red shift in the UV absorption band of TCP, no changes in K(b) values in the presence of salt, no significant changes in the specific viscosity and CD spectra of DNA, and a decrease in peak currents with no shift in the voltamogram. In addition, TCP is able to release Hoechst 33258, a strong groove binder, in the DNA solutions. The results are indicative of the groove-binding mode of TCP to DNA.

The interaction of two new water-soluble [Cu(4,7-dmp)(phen-dione)Cl]Cl (1) and [Cu(2,9-dmp)(phen-dione)Cl]Cl (2) which dmp is dimethyl-1,10-phenanthroline and phen-dion represents 1,10-phenanthroline-5,6-dion, with DNA in solution and immobilized DNA on a chitosan-carbon nanotubes composite modified glassy carbon electrode were investigated by cyclic voltammetry and UV-Vis spectroscopy techniques. In solution interactions, spectroscopic and electrochemical evidences indicate outside binding of these complexes. To clarify the binding mode of complexes, it was done competition studies with Hoechst and Neutral red as groove binder and intercalative probes, respectively. All these results indicating that, these two complexes (1) and (2) interact with DNA via groove binding and partially intercalative modes, respectively. The electrochemical characterization experiments showed that the nanocomposite film of chitosan-carbon nanotubes could effectively immobilize DNA and greatly improve the electron-transfer reactions of the electroactive molecules that latter finding is the result of strong interactions between captured DNA and Cu complexes. This result indicates that these complexes could be noble candidates as hybridization indicators in further studies. At the end, these new complexes showed excellent antitumor activity against K562 (human chronic myeloid leukemia) cell lines.

The interaction of native calf thymus DNA with the Pd(II) complex, PdCl2(LL) (LL = chelating diamine ligand: N,N-dimethyltrimethylenediamine), in 10 mM Hepes aqueous solutions at neutral pH has been monitored as a function of metal complex/DNA molar ratio by UV absorption spectrophotometry, circular dichroism (CD), viscosimetry, and fluorescence spectroscopy. The results support two modes of interaction. In particular, this complex showed absorption hypochromism and then hyperchromism, increase in melting temperature, and some structural changes in specific viscosity when bound to calf thymus DNA. The binding constant determined using absorption measurement is 2.69·103 M−1. As evidenced by the increasing fluorescence of methylene blue-DNA solutions in the presence of increasing amounts of metal complex, PdCl2(LL) is able to displace the methylene blue intercalated into DNA, but not so completely, as indicated by partial intercalation. CD spectral changes in two steps and viscosity decrease confirm our conclusions.

The [PtCl2(LL)] complex, as a cisplatin derivative, which LL is diamine chelate ligand (N,N-dimethyltrimethylendiamine), was synthesized and characterized by elemental analysis (CHN) mass, 1H, and 13C nuclear magnetic resonance techniques. Then the binding of this complex to calf thymus DNA was investigated by various physicochemical methods such as spectrophotometric, circular dichroism, spectrofluorometric, melting temperature, and viscosimetric techniques. Upon addition of the complex, important changes were observed in the characteristic UV–Vis bands (hypochromism) of calf thymus DNA, increase in melting temperature and some changes in specific viscosity. Also, the fluorescence spectral characteristics showed an increase in the fluorescence intensity of methylene blue–DNA solutions in the presence of increasing amounts of metal complex, indicating PtCl2(LL) is able to displace the methylene blue bound to DNA but not as complete as intercalative molecules. The experimental results showed that the platinum complex is bound to DNA non-intercalatively, and an outside binding is the preferred mode of interaction.