Research Interests: Engineering, Genetics, Biology, Cell Phones, DNA replication, and 15 moreBioelectromagnetics, Epigenetics, Biological Sciences, Electromagnetic Radiation, Electromagnetic Fields, Aneuploidy, DNA Array, Electromagnetic Field, Finite element simulation, Chromosomal Instability, Elevated Temperature, centromere, chromosome instability, Chromosome aberrations, and Cytogenetic analysis
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1. 1. A method for the isolation of haemolysins and ichthyotoxins from Ochromonas using various Chromatographie methods is described. ... 2. 2. The toxins were polar substances which formed a red colour with rhodamine 6G, soluble in water... more
1. 1. A method for the isolation of haemolysins and ichthyotoxins from Ochromonas using various Chromatographie methods is described. ... 2. 2. The toxins were polar substances which formed a red colour with rhodamine 6G, soluble in water as well as in organic solvents.
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Among the major crops, cereals constitute about 50% of the annual world protein production used for animal and human consumption (Hanson et al. 1982). Among cereals, wheat production exceeds all other crops, accounting for about 40% of... more
Among the major crops, cereals constitute about 50% of the annual world protein production used for animal and human consumption (Hanson et al. 1982). Among cereals, wheat production exceeds all other crops, accounting for about 40% of the total protein production. Wheat, therefore, has the major contribution of any single crop to the world protein production (Harlan and Starks 1980). Naturally, the least expensive and most effective means of increasing protein production and upgrading its quality is through the improvement of wheat protein. This chapter deals primarily with the quantitative aspects of high protein wheat.
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A novel approach for establishing common or random integration loci for retroviral genomes
Pengambilan keputusan sangat diperlukan oleh hakim dalam menentukan putusan yang akan dijatuhkan kepada terdakwa. Hakim harus dapat mengolah dan memproses data-data yang diperoleh selama proses persidangan dalam hal ini bukti- bukti,... more
Pengambilan keputusan sangat diperlukan oleh hakim dalam menentukan putusan yang akan dijatuhkan kepada terdakwa. Hakim harus dapat mengolah dan memproses data-data yang diperoleh selama proses persidangan dalam hal ini bukti- bukti, keterangan saksi, pembelaan terdakwa, serta tuntutan jaksa maupun muatan psikologis. Keputusan yang akan dijatuhkan kepada terdakwa dapat didasari oleh rasa tanggung jawab, keadilan, kebijaksanaan, profesionalisme dan bersifat obyektif. Meskipun sistem hukum terkadang tidak dapat mencapai keadilan yang sempurna, namun hakim harus dapat menetapkan keputusan yang mendekati keadilan. Di negara demokrasi, yang terpenting perselisihan diatasi dengan cara yang tampak adil dan mendukung stabilitas sosial. Pada kenyataannya ada saja yang mungkin tidak setuju dengan keputusan yang dijatuhkan oleh pengadilan, namun masyarakat harus percaya pada keadilan sistem hukum secara keseluruhan. Tujuan Penelitian ini untuk mengetahui bagaimana proses pengambilan keputusan hakim dalam perkara pidana dan faktor-faktor yang mempengaruhi suatu keputusan pengadilan. Pertanyaan penelitian pada penelitian ini adalah bagaimana proses pengambilan keputusan seorang hakim dan faktor-faktor yang mempengaruhi pengambilan keputusan seorang hakim. Fokus gejala dalam penelitian ini adalah mengenai proses pengambilan keputusan hakim. Subjek dalam penelitian ini adalah empat orang hakim pengadilan negeri kota mungkid yang telah memenuhi karakteristik penelitian. Metode yang digunakan metode penelitian kualitatif, alat pengumpulan data menggunakan metode observasi dan interview serta biodata diri. Berdasarkan penelitian diperoleh hasil, proses pengambilan keputusan hakim (putusan) perkara pidana harus berdasarkan Hukum Acara Pidana. Proses pemikiran hakim melalui tahap intelegensia, tahap desain dan tahap pemilihan. Faktor yang dapat mempengaruhi suatu putusan adalah faktor internal diantaranya usia, keterampilan berkomunikasi, pengetahuan hakim, ketajaman intuisi, intelegensi, kemampuan mengungkap fakta, ketangkasan mengungkap fakta, menguasai norma hukum, rasa keadilan bagi masyarakat, keluarga, korban dan terdakwa, ketajaman mengaitkan fakta dan norma dan pengalaman. Faktor eksternal berupa tekanan sosial seperti tekanan dari pejabat, diberi uang, akan ditembak, pendidikan, pelatihan, hal yang memberatkan dan meringankan. Faktor lain yang berpengaruh yaitu faktor religiusitas, alat bukti, keyakinan, undangundang, fakta, rasa keadilan serta disparitas putusan.
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The aberrant asynchronous replication — characterizing lymphocytes of cancer patients — is erased following stem cell transplantation
Research Interests: Biochemistry, Bioinformatics, Biophysics, Chemistry, Chinese Studies, and 15 moreComputational Biology, Cancer, Breast Cancer, Agriculture, China, Continuous Improvement, Cancer Biology, Biomineralization, Colorectal cancer, Chicken, Cancer Screening, Delay, Cancer Patient, Cancer Registry, and Cause of death
Uniparental disomy (UPD), an altered mode of Mendelian inheritance, may reveal expression of recessive alleles due to the loss of heterozygosity, as well as imprinted genes. The mechanism causing UPD can be best elucidated in offsprings... more
Uniparental disomy (UPD), an altered mode of Mendelian inheritance, may reveal expression of recessive alleles due to the loss of heterozygosity, as well as imprinted genes. The mechanism causing UPD can be best elucidated in offsprings of individuals at high risk for chromosomal non-disjunction. Such individuals are Familial Mediterranean Fever (FMF) patients, who are routinely treated with the antimitotic agent colchicine, and, therefore, are expected to be at an increased risk for aneuploidy. A dominant mode of inheritance was observed in four FMF offsprings having one parent exhibiting the FMF phenotype (homozygote recessive) while the other was free of the mutant allele (as assumed from his ethnic background). Out of these, two exhibited UPD of chromosome 16, which carries the FMF gene, as judged from four different RFLP markers along this chromosome. Since in both case the UPD was of maternal origin, it is suggested that the colchicine-treated FMF mothers contributed two doses...
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Replication timing of the genetic material is a highly programmed process correlated with expression, stability and methylation capacity. An important aspect of that timing is the temporal order of allelic replication: a synchronous mode... more
Replication timing of the genetic material is a highly programmed process correlated with expression, stability and methylation capacity. An important aspect of that timing is the temporal order of allelic replication: a synchronous mode for biallelically expressed genes and an asynchronous for monoallelically expressed genes. Previous studies showed that malignancy is associated with changes in the inherent mode of allelic replication, and even normal cells of cancer patients display alterations in the replication of various genes. Using fluorescence in situ hybridization (FISH), we checked whether allelic-replication mode differentiates cancer patients from healthy individuals. We focused on prostate cancer (CAP), the most common diagnosed cancer and the second leading cause of cancer death in men over 50 years old. Five nonrelated genes and a nontranscribed DNA sequence associated with chromosomal segregation were used in our study. All 6 tested loci displayed in peripheral blood lymphocytes stimulated with phytohemagglutinin (PHA) of CAP patients loss of their inherent temporal order of allelic replication, coupled with aneuploidy, the outcome of chromosome malsegregation. The replication-timing modification is a reversible epigenetic alteration, evidenced by our ability to resurrect the normal pattern in all 6 tested loci by introducing an inhibitor of methyl transferase. On the other hand, the methylation-blocking agent failed to obliterate aneuploidy. The replication alteration accompanied by aneuploidy, detected in peripheral blood cells, distinguishes between CAP patients and individuals with benign prostate hyperplasia (BPH; a common disorder in elderly men) better than the routinely used blood marker, the prostate-specific antigen (PSA).
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The effect of colchicine at concentrations of 0.25 X 10(-6) M, 1.0 X 10(-6) M, and 2.0 X 10(-6) M on the degree of satellite association (SA) was estimated in phytohemagglutinin-stimulated lymphocytes of individuals in the following... more
The effect of colchicine at concentrations of 0.25 X 10(-6) M, 1.0 X 10(-6) M, and 2.0 X 10(-6) M on the degree of satellite association (SA) was estimated in phytohemagglutinin-stimulated lymphocytes of individuals in the following groups: cystic fibrosis (CF) children, obligatory CF heterozygotes, control children, and control adults. In all four groups increasing colchicine concentration caused a higher degree of SA. The degree of SA differed between the two control age groups (children vs adults) only at the lowest concentration. CF patients had a significantly higher degree of SA than CF heterozygotes and than control individuals at all colchicine concentrations; CF heterozygotes had a significantly higher degree of SA than control adults at the low and intermediate concentrations. There was a strong interaction between genotype and colchicine concentration: the differences between the CF patients and the control individuals were most distinct at the intermediate concentration and between the carriers and the control individuals at the low colchicine concentration. Colchicine had no effect on the activity of the nucleolar organizer regions (NORs), as measured by the frequency of the silver-stained NORs (AgNORs), while the frequency of AgNORs in CF patients was significantly lower as compared to control individuals. Yet, the increase in the degree of SA caused by the CF mutant allele involved specifically the satellited chromosomes carrying active NORs.
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Following the application of two-color fluorescence in-situ hybridization (FISH) to human interphase cells, we examined the replication timing of the fragile-X locus relative to the non-transcribed late replicating alpha-satellite region... more
Following the application of two-color fluorescence in-situ hybridization (FISH) to human interphase cells, we examined the replication timing of the fragile-X locus relative to the non-transcribed late replicating alpha-satellite region of chromosome-X, a built-in intracellular reference locus. In this assay, an unreplicated locus is identified by a single hybridization signal (singlet; S), whereas a replicated locus is identified by a duplicated signal (doublet; D). Hence, following simultaneous hybridization with the FMR1 and alpha-satellite probes, male cells with one singlet and one doublet signal per cell (SD cells) indicate S-phase cells where only one of the two loci has replicated. The studied cell samples (lymphocytes and amniocytes) were derived from normal males, fragile-X male patients, and premutation male carriers. Three distinct populations of SD cells were identified among the various samples. The first population had a high frequency of cells showing a doublet FMR1; this pattern, indicating early replication of FMR1, characterized the SD cell population of normal males. The second population had a high frequency of cells showing a singlet FMR1; this pattern, indicating very late replication of FMR1, characterized the SD population of fragile-X patients. The third population had about one half of the cells showing a singlet FMR1 and the other half with a doublet FMR1, indicating somatic variation in the replication timing of FMR1; this pattern was seen in the SD cell population of premutation carriers. The replication status of the FMR1 locus in the cells of patients was altered from late to early in the presence of 5-azadeoxycytidine, an activator of various silent genes. Based on the vast amount of information showing that expressed loci replicate early, whereas unexpressed loci replicate late, we inferred from the replication status of the FMR1 locus that: (1) the normal FMR1 allele is transcriptionally active in lymphocytes and amniocytes; (2) the fully mutated FMR1 allele is transcriptionally silent; (3) the transcriptional activity of the premutated allele is somewhat disturbed; (4) 5-azadeoxycytidine activates the fully mutated FMR1 allele.
Research Interests: Genetics, Human Genetics, Complementary and Alternative Medicine, DNA replication, Gene expression, and 12 moreHigh Frequency, Humans, Fluorescence in situ hybridization, Mutation, Male, Fragile X Syndrome, Replication, Fluorescent in situ hybridization, RNA-binding proteins, Lymphocytes, alleles, and Paediatrics and reproductive medicine
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Different genotypes of Triticum longissimum are known to either promote or suppress chromosome pairing in crosses with polyploid wheats. Lines that promote homoeologous pairing are here designated as intermediate pairing lines, while... more
Different genotypes of Triticum longissimum are known to either promote or suppress chromosome pairing in crosses with polyploid wheats. Lines that promote homoeologous pairing are here designated as intermediate pairing lines, while those which have no such effect or suppress pairing are known as low pairing lines. To determine a possible effect of these genotypes on homologous pairing, tetraploidy was induced in both lines and chromosomal pairing was studied at first metaphase of meiosis. While the two induced autotetraploids did not differ in chiasma frequency or in the number of paired chromosomal arms, they differed significantly in multivalent frequency; the intermediate-pairing autotetraploid exhibited the same multivalent frequency as that expected on the basis of two telomeric initiation sites, while the low pairing autotetraploid exhibited a significantly lower frequency. It is assumed that in the autotetraploid the low pairing genotype does not affect meiotic pairing per ...
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The low-pairing gene or genes of diploid Triticum longissimum (Schwinf. &Muschl.) Bowden predispose the induced autotetraploid towards bivalent pairing. In this work, bivalentization was phenocopied in intermediate- and low-pairing lines... more
The low-pairing gene or genes of diploid Triticum longissimum (Schwinf. &Muschl.) Bowden predispose the induced autotetraploid towards bivalent pairing. In this work, bivalentization was phenocopied in intermediate- and low-pairing lines of T. longissimum and in a high-pairing line of T. speltoides (Tausch) Gren. ex Richter, by colchicine treatment during the last premeiotic mitosis. This treatment induced C-mitosis and tetraploid cells which are characterized by almost exclusive bivalent pairing instead of the expected multivalent pairing. Colchicine disrupted the association of homologous chromosomes in the premeiotic metaphase but left the sister chromatids located close to each other. As a result, rather than being all closely associated, the four homologues were arranged in pairs already prior to meiosis. The effect of colchicine in this respect is reminiscent of that of the "diploidizing genes" in many naturally occurring polyploids. This work demonstrates once again...
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In a plant of Triticum aestivum L. em. Thell. cultivar Chinese Spring which was disomic for a mutant isochromosome of the long arm of chromosome 5D (di-isosomic 5DLM), partial chromosome asynapsis was detected at meiosis. Chromosome... more
In a plant of Triticum aestivum L. em. Thell. cultivar Chinese Spring which was disomic for a mutant isochromosome of the long arm of chromosome 5D (di-isosomic 5DLM), partial chromosome asynapsis was detected at meiosis. Chromosome pairing in F1 hybrids from crosses of T. aestivum plants carrying the mutant isochromosome with Secale cereale, an intermediate pairing line of T. longissimum and with T. sharonensis disclosed that 5DLM carried a gene that reduced homoeologous chromosome pairing. This gene, designated Ph3 is less potent than its assumed homoeoallele on chromosomal arm 5BL, i.e., Ph1. The possibility of Ph1 being transferred from 5BL to 5DL through homoeologous chromosome pairing and recombination was discarded. Rather, it seems more likely that this allele resulted from a spontaneous mutation of the pairing-promoter allele known to be located on 5DL.
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Lines of common wheat cv. 'Chinese Spring' carrying different doses of the Ph1 gene, located on the long arm of chromosome 5B were treated with the antimitotic agents griseofulvin and isopropyl-N-phenyl-carbamate (IPC). Treatments... more
Lines of common wheat cv. 'Chinese Spring' carrying different doses of the Ph1 gene, located on the long arm of chromosome 5B were treated with the antimitotic agents griseofulvin and isopropyl-N-phenyl-carbamate (IPC). Treatments with low griseofulvin concentrations and IPC resulted mainly in the production of a high percentage of cells exhibiting spindle disorganization at metaphase and multipolar cells at anaphase–telophase. These treatments did not differentially affect the tested genotypes. On the other hand, higher griseofulvin concentrations induced the appearance of frequent C-metaphases and C-anaphases because of complete disruption of the spindle microtubules; in such cases, more pronounced sensitivity was observed in a line lacking the Ph1 gene than in lines disomic and tetrasomic for chromosome 5B. From this evidence it can be concluded that subcellular structures regulating microtubule organization and orientation, which are apparently the target of IPC and low ...
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Mitotic spindle sensitivity to colchicine of a common wheat line carrying a mutation at the Ph1 locus which increases homoeologous pairing at meiosis, was studied in treated root-tip cells and compared with that of wild type (Ph1/Ph1) and... more
Mitotic spindle sensitivity to colchicine of a common wheat line carrying a mutation at the Ph1 locus which increases homoeologous pairing at meiosis, was studied in treated root-tip cells and compared with that of wild type (Ph1/Ph1) and plants deficient for chromosome 5B. Spindle sensitivity was determined by the percentage of fully affected cells of the overall metaphase cell population. The high-pairing mutant (ph1b/ph1b), which is assumed to be a deficiency for the Ph1 locus on the long arm of chromosome 5B, was found to be significantly more sensitive to colchicine than the corresponding control line, i.e., plants carrying the wild-type allele, Ph1. The sensitivity of the mutant was similar to that of plants deficient for chromosome 5B. It thus becomes highly probable that spindle sensitivity to colchicine, previously found in plants carrying different doses of chromosomal arm 5BL, is indeed determined by the Ph1 gene. This gene presumably exerts its effect on the spindle via ...
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Reciprocal crosses were made between cultivated wheat (Triticum turgidum var. 'durum') and a high-protein line of wild tetraploid wheat (T. turgidum var. 'dicoccoides'). F1 grains (on maternal spikes) were very similar to... more
Reciprocal crosses were made between cultivated wheat (Triticum turgidum var. 'durum') and a high-protein line of wild tetraploid wheat (T. turgidum var. 'dicoccoides'). F1 grains (on maternal spikes) were very similar to the selfed grains on the maternal parent in protein percentage, weight and protein content. These traits were also analyzed in F3 grains developed on F2 spikes of segregating populations derived from reciprocal crosses between the same cultivated parent and another high-protein line of var. 'dicoccoides'. No significant differences in the mean values of these traits were found between the reciprocal crosses, indicating no cytoplasmic effect. It has been concluded that these grain characteristics are largely determined by the maternal plant.
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Peripheral blood lymphocytes of patients with hematological malignancies or solid tumors, such as renal cell carcinoma or prostate cancer, display epigenetic aberrations (loss of synchronous replication of allelic counterparts) and... more
Peripheral blood lymphocytes of patients with hematological malignancies or solid tumors, such as renal cell carcinoma or prostate cancer, display epigenetic aberrations (loss of synchronous replication of allelic counterparts) and genetic changes (aneuploidy) characteristic of the cancerous phenotype. This study sought to determine whether such alterations could differentiate breast cancer patients from cancer-free subjects. The HER2 locus-an oncogene assigned to chromosome 17 whose amplification is associated with breast cancer (BCA)-and the pericentromeric satellite sequence of chromosome 17 (CEN17) were used for replication timing assessments. Aneuploidy was monitored by enumerating the copy numbers of chromosome 17. Replication timing and aneuploidy were detected cytogenetically using fluorescence in situ hybridization technology applied to phytohemagglutinin-stimulated lymphocytes of 20 women with BCA and 10 control subjects. We showed that both the HER2 and CEN17 loci in the ...
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The process by which homologous chromosomes recognize each other at the beginning of meiosis, prior to synapsis, is poorly understood. To gain a better understanding as to when, where and how a given chromosome approaches its pairing... more
The process by which homologous chromosomes recognize each other at the beginning of meiosis, prior to synapsis, is poorly understood. To gain a better understanding as to when, where and how a given chromosome approaches its pairing partner, chromosome behavior at early stages of meiosis in human spermatocytes was studied. Using multi-color FISH with centromeric- and telomeric-specific probes, as well as with whole chromosome DNA libraries, it was clearly aligned. Rather, similarly to non-homologous chromosomes, they were well separated from each other. At the commencement of synapsis, during the process of homology search, homologues underwent a drastic conformational change, elongating into strands that approached each other by their telomeres. Just preceding the co-alignment of the homologous centromeres, telomeres changed their interphase random distribution and occupied a confined region of the nuclear periphery. Following synapsis, telomeres spread over the whole nuclear peri...
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ABSTRACT
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Background The temporal order of allelic replication is interrelated to the epigenomic profile. A significant epigenetic marker is the asynchronous replication of monoallelically-expressed genes versus the synchronous replication of... more
Background The temporal order of allelic replication is interrelated to the epigenomic profile. A significant epigenetic marker is the asynchronous replication of monoallelically-expressed genes versus the synchronous replication of biallelically-expressed genes. The present study sought to determine whether a microdeletion in the genome affects epigenetic profiles of genes unrelated to the missing segment. In order to test this hypothesis, we checked the replication patterns of two genes – SNRPN, a normally monoallelically expressed gene (assigned to 15q11.13), and the RB1, an archetypic biallelically expressed gene (assigned to 13.q14) in the genomes of patients carrying the 22q11.2 deletion (DiGeorge/Velocardiofacial syndrome) and those carrying the 7q11.23 deletion (Williams syndrome). Results The allelic replication timing was determined by fluorescence in situ hybridization (FISH) technology performed on peripheral blood cells. As expected, in the cells of normal subjects the ...
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... Authors: Korenstein, A.; Avivi, L. [Tel-Avivi University (Israel)]; Ravia, Y. [Sheba Medical Center, Tel-Hashomer (Israel)]. Publication Date: 1994 Sep 01. OSTI Identifier: OSTI ID: 133705. Report Number(s): CONF-941009--. Resource... more
... Authors: Korenstein, A.; Avivi, L. [Tel-Avivi University (Israel)]; Ravia, Y. [Sheba Medical Center, Tel-Hashomer (Israel)]. Publication Date: 1994 Sep 01. OSTI Identifier: OSTI ID: 133705. Report Number(s): CONF-941009--. Resource Type: Journal Article. ...
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Research Interests: Genetics, Human Genetics, Complementary and Alternative Medicine, DNA replication, Adolescent, and 15 moreHumans, Fluorescence in situ hybridization, Genetic Testing, Mutation, Female, Amniotic Fluid, Fluorescent in situ hybridization, Adult, RNA-binding proteins, X chromosome, Lymphocytes, X chromosome inactivation, DNA sequence, alleles, and Paediatrics and reproductive medicine
The relationship between chromosomal nondisjunction and semen quality was studied in two groups of males who differ highly in their semen quality: 12 individuals with low-quality semen caused by varicocele, and 8 subjects with... more
The relationship between chromosomal nondisjunction and semen quality was studied in two groups of males who differ highly in their semen quality: 12 individuals with low-quality semen caused by varicocele, and 8 subjects with high-quality semen, selected from sperm donors for in vitro fertilization. Chromosomal nondisjunction was inferred from the rate of disomy found in mature sperm cells. To determine the rate of disomy, we applied fluorescence in situ hybridization using satellite-specific probes for chromosomes 1, 15, 18, X and Y. In sperm cells of males with low-quality semen, the mean rate of disomy for each of the autosomes and of hetero-disomy for the sex chromosomes (XY) was significantly higher than that observed in the high-quality semen samples: more than 15-fold higher for chromosomes 1 and 15, and 7-fold higher for chromosomes 18 and XY. Yet, the homo-disomy rate for each of the sex chromosomes (XX and YY) was almost the same in both types of semen. The large discrepancy between the low- and high-quality semen in the rate of sex chromosome hetero-disomy versus the similar rate of homo-disomy strongly suggests that the abnormal chromosomal segregation in meiocytes of males with low-quality semen resulted from chromosomal nondisjunction at the first meiotic division. The results indicate that men showing poor semen quality are at an increased risk for meiotic nondisjunction, similar to women at the end of their reproductive years.
Research Interests: Genetics, Human Genetics, Complementary and Alternative Medicine, Humans, Meiosis, and 15 moreIn Vitro Fertilization, Male, Y chromosome, Risk factors, Fluorescent in situ hybridization, Adult, Chromosome segregation, X chromosome, Fertilization in Vitro, Risk Factors, Semen, Spermatozoa, Semen Quality, Sex Chromosome, and Paediatrics and reproductive medicine
Following the application of two-color fluorescence in-situ hybridization (FISH) to human interphase cells, we examined the replication timing of the fragile-X locus relative to the non-transcribed late replicating alpha-satellite region... more
Following the application of two-color fluorescence in-situ hybridization (FISH) to human interphase cells, we examined the replication timing of the fragile-X locus relative to the non-transcribed late replicating alpha-satellite region of chromosome-X, a built-in intracellular reference locus. In this assay, an unreplicated locus is identified by a single hybridization signal (singlet; S), whereas a replicated locus is identified by a duplicated signal (doublet; D). Hence, following simultaneous hybridization with the FMR1 and alpha-satellite probes, male cells with one singlet and one doublet signal per cell (SD cells) indicate S-phase cells where only one of the two loci has replicated. The studied cell samples (lymphocytes and amniocytes) were derived from normal males, fragile-X male patients, and premutation male carriers. Three distinct populations of SD cells were identified among the various samples. The first population had a high frequency of cells showing a doublet FMR1; this pattern, indicating early replication of FMR1, characterized the SD cell population of normal males. The second population had a high frequency of cells showing a singlet FMR1; this pattern, indicating very late replication of FMR1, characterized the SD population of fragile-X patients. The third population had about one half of the cells showing a singlet FMR1 and the other half with a doublet FMR1, indicating somatic variation in the replication timing of FMR1; this pattern was seen in the SD cell population of premutation carriers. The replication status of the FMR1 locus in the cells of patients was altered from late to early in the presence of 5-azadeoxycytidine, an activator of various silent genes. Based on the vast amount of information showing that expressed loci replicate early, whereas unexpressed loci replicate late, we inferred from the replication status of the FMR1 locus that: (1) the normal FMR1 allele is transcriptionally active in lymphocytes and amniocytes; (2) the fully mutated FMR1 allele is transcriptionally silent; (3) the transcriptional activity of the premutated allele is somewhat disturbed; (4) 5-azadeoxycytidine activates the fully mutated FMR1 allele.
Research Interests: Genetics, Human Genetics, Complementary and Alternative Medicine, DNA replication, Gene expression, and 12 moreHigh Frequency, Humans, Fluorescence in situ hybridization, Mutation, Male, Fragile X Syndrome, Replication, Fluorescent in situ hybridization, RNA-binding proteins, Lymphocytes, alleles, and Paediatrics and reproductive medicine
In this study, in order to evaluate the replication pattern and the cell cycle dynamics of normal and malignant cells from patients with chronic lymphocytic leukemia, we applied the FISH technique with the p53 gene. Asynchrony was... more
In this study, in order to evaluate the replication pattern and the cell cycle dynamics of normal and malignant cells from patients with chronic lymphocytic leukemia, we applied the FISH technique with the p53 gene. Asynchrony was determined by the presence of one single and one set of double dots in the same cell. The rate of asynchronous replication was significantly higher in malignant cells than in normal cells (a mean of 28 vs 13, respectively, P = 0.023). There were proportionately more cells with two single dots among the normal cells (P = 0.0047). These results probably reflect the changes in gene replication and cell cycle progression that occur in malignant cells.
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Research Interests: Information Systems, Genetics, Genomics, Gene regulation, Transcription Factors, and 15 moreGene expression, Down Syndrome, Cell line, Humans, Fluorescence in situ hybridization, Mice, Animals, Genetic Map, Transcription Factor, Molecular cloning, Amino Acid Sequence, Gene Family, DNA binding proteins, Molecular Sequence Data, and Sequence similarity
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Modification in the inherent mode of allelic replication in lymphocytes of patients suffering from renal cell carcinoma: A novel genetic alteration associated with malignancyThis paper is based on a portion of a dissertation to be submitted by Zohar A. Dotan in partial fulfillment of the requirem...more
... 2 Department of Urology, Sheba Medical Center, Tel-Hashomer, Israel. Email: Lydia Avivi (lydia@post.tau.ac.il). *Correspondence: Lydia Avivi, Department of Human Genetics, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv... more
... 2 Department of Urology, Sheba Medical Center, Tel-Hashomer, Israel. Email: Lydia Avivi (lydia@post.tau.ac.il). *Correspondence: Lydia Avivi, Department of Human Genetics, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv 699788, Israel. ...
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... Address reprint requests to Lydia Avivi, Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel ... The use of interphase nuclei for chromosomal and DNA studies, an approach termed... more
... Address reprint requests to Lydia Avivi, Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel ... The use of interphase nuclei for chromosomal and DNA studies, an approach termed interphase cytogen-etics (Cremer et al., ...