Research Interests:
Research Interests:
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic... more
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic disorders associated with obesity. The aim of this study was to evaluate the 11β-HSD1 expression in liver, visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in morbidly obese patients undergoing bariatric surgery and its correlations with clinical, anthropometric, and biochemical variables. Methods A prospective study was conducted over a 27-month period. Hepatic, VAT, and SAT samples were obtained at the time of surgery. 11β-HSD1 and 18S gene expression was measured using real-time quantitative reverse transcriptase-polymerase chain reaction. Results Forty nine patients met the inclusion criteria [mean age: 42.2 ± 10 years, body mass index (BMI): 42 ± 6 kg/m2, 71% women and 63% with metabolic syndrome (MS)]. 11β-HSD1 mRNA levels were higher in liver than fat tissue (p < 0.001), being higher in SAT than in VAT (p < 0.001) without gender-specific differences. Hepatic expression of 11β-HSD1 correlated positively with SAT and VAT, alanine aminotransferase (ALT), and serum glucose and was inversely associated with BMI. 11β-HSD1 mRNA in VAT correlated positively with insulinemia, ALT, and LDL cholesterol. There were no associations between 11β-HSD1 mRNA in SAT and the variables analyzed. Conclusions 11β-HSD1 expression is higher in liver in comparison to adipose tissue in obese patients. The observed correlations between hepatic and VAT 11β-HSD1 expression with dyslipidemia and insulin resistance suggest that this enzyme might have a pathogenic role in obesity and related metabolic disorders.
Research Interests: Bariatric Surgery, Obesity, Gene expression, Metabolic syndrome, Insulin Resistance, and 35 morereal time PCR, Adipose tissue, Cortisol, Humans, Liver, Mice, Cholesterol, Animals, Corticosterone, Young Adult, non alcoholic fatty liver disease (NAFLD), Body Mass Index, ROC Curve, Obesity Surgery, Triglycerides, LDL-cholesterol, Enzyme, Clinical Sciences, Fatty Liver, Fat Mass, Body mass index (BMI), Public health systems and services research, Alanine Aminotransferase, Odds ratio, Western blot, Protein Expression, Metabolic Disorder, Abdominal Obesity, Fasting Insulin, Enzyme Linked Immunosorbent Assay, Glucocorticoids, Prospective Study, Cushing Syndrome, Gene expression profiling, and Alanine Transaminase
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic... more
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic disorders associated with obesity. The aim of this study was to evaluate the 11β-HSD1 expression in liver, visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in morbidly obese patients undergoing bariatric surgery and its correlations with clinical, anthropometric, and biochemical variables. Methods A prospective study was conducted over a 27-month period. Hepatic, VAT, and SAT samples were obtained at the time of surgery. 11β-HSD1 and 18S gene expression was measured using real-time quantitative reverse transcriptase-polymerase chain reaction. Results Forty nine patients met the inclusion criteria [mean age: 42.2 ± 10 years, body mass index (BMI): 42 ± 6 kg/m2, 71% women and 63% with metabolic syndrome (MS)]. 11β-HSD1 mRNA levels were higher in liver than fat tissue (p < 0.001), being higher in SAT than in VAT (p < 0.001) without gender-specific differences. Hepatic expression of 11β-HSD1 correlated positively with SAT and VAT, alanine aminotransferase (ALT), and serum glucose and was inversely associated with BMI. 11β-HSD1 mRNA in VAT correlated positively with insulinemia, ALT, and LDL cholesterol. There were no associations between 11β-HSD1 mRNA in SAT and the variables analyzed. Conclusions 11β-HSD1 expression is higher in liver in comparison to adipose tissue in obese patients. The observed correlations between hepatic and VAT 11β-HSD1 expression with dyslipidemia and insulin resistance suggest that this enzyme might have a pathogenic role in obesity and related metabolic disorders.
Research Interests: Bariatric Surgery, Obesity, Gene expression, Metabolic syndrome, Insulin Resistance, and 35 morereal time PCR, Adipose tissue, Cortisol, Humans, Liver, Mice, Cholesterol, Animals, Corticosterone, Young Adult, non alcoholic fatty liver disease (NAFLD), Body Mass Index, ROC Curve, Obesity Surgery, Triglycerides, LDL-cholesterol, Enzyme, Clinical Sciences, Fatty Liver, Fat Mass, Body mass index (BMI), Public health systems and services research, Alanine Aminotransferase, Odds ratio, Western blot, Protein Expression, Metabolic Disorder, Abdominal Obesity, Fasting Insulin, Enzyme Linked Immunosorbent Assay, Glucocorticoids, Prospective Study, Cushing Syndrome, Gene expression profiling, and Alanine Transaminase
Research Interests: Bariatric Surgery, Metabolism, Lipids, Cortisol, Humans, and 21 moreBlood Glucose, Insulin, Liver, Female, Male, Body Mass Index, Enzyme, Clinical Sciences, Aged, Middle Aged, C reactive protein, Adiponectin, Adult, Body Mass, Central Obesity, Biological markers, Adaptive Response, Metabolic Disorder, Aspartate Aminotransferases, Cushing Syndrome, and Alanine Transaminase
Research Interests: Bariatric Surgery, Metabolism, Lipids, Cortisol, Humans, and 21 moreBlood Glucose, Insulin, Liver, Female, Male, Body Mass Index, Enzyme, Clinical Sciences, Aged, Middle Aged, C reactive protein, Adiponectin, Adult, Body Mass, Central Obesity, Biological markers, Adaptive Response, Metabolic Disorder, Aspartate Aminotransferases, Cushing Syndrome, and Alanine Transaminase
Research Interests:
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic... more
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic disorders associated with obesity. The aim of this study was to evaluate the 11β-HSD1 expression in liver, visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in morbidly obese patients undergoing bariatric surgery and its correlations with clinical, anthropometric, and biochemical variables. Methods A prospective study was conducted over a 27-month period. Hepatic, VAT, and SAT samples were obtained at the time of surgery. 11β-HSD1 and 18S gene expression was measured using real-time quantitative reverse transcriptase-polymerase chain reaction. Results Forty nine patients met the inclusion criteria [mean age: 42.2 ± 10 years, body mass index (BMI): 42 ± 6 kg/m2, 71% women and 63% with metabolic syndrome (MS)]. 11β-HSD1 mRNA levels were higher in liver than fat tissue (p < 0.001), being higher in SAT than in VAT (p < 0.001) without gender-specific differences. Hepatic expression of 11β-HSD1 correlated positively with SAT and VAT, alanine aminotransferase (ALT), and serum glucose and was inversely associated with BMI. 11β-HSD1 mRNA in VAT correlated positively with insulinemia, ALT, and LDL cholesterol. There were no associations between 11β-HSD1 mRNA in SAT and the variables analyzed. Conclusions 11β-HSD1 expression is higher in liver in comparison to adipose tissue in obese patients. The observed correlations between hepatic and VAT 11β-HSD1 expression with dyslipidemia and insulin resistance suggest that this enzyme might have a pathogenic role in obesity and related metabolic disorders.
Research Interests: Bariatric Surgery, Obesity, Gene expression, Metabolic syndrome, Insulin Resistance, and 35 morereal time PCR, Adipose tissue, Cortisol, Humans, Liver, Mice, Cholesterol, Animals, Corticosterone, Young Adult, non alcoholic fatty liver disease (NAFLD), Body Mass Index, ROC Curve, Obesity Surgery, Triglycerides, LDL-cholesterol, Enzyme, Clinical Sciences, Fatty Liver, Fat Mass, Body mass index (BMI), Public health systems and services research, Alanine Aminotransferase, Odds ratio, Western blot, Protein Expression, Metabolic Disorder, Abdominal Obesity, Fasting Insulin, Enzyme Linked Immunosorbent Assay, Glucocorticoids, Prospective Study, Cushing Syndrome, Gene expression profiling, and Alanine Transaminase
Research Interests:
Research Interests: Bariatric Surgery, Metabolism, Lipids, Cortisol, Humans, and 21 moreBlood Glucose, Insulin, Liver, Female, Male, Body Mass Index, Enzyme, Clinical Sciences, Aged, Middle Aged, C reactive protein, Adiponectin, Adult, Body Mass, Central Obesity, Biological markers, Adaptive Response, Metabolic Disorder, Aspartate Aminotransferases, Cushing Syndrome, and Alanine Transaminase
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic... more
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic disorders associated with obesity. The aim of this study was to evaluate the 11β-HSD1 expression in liver, visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in morbidly obese patients undergoing bariatric surgery and its correlations with clinical, anthropometric, and biochemical variables. Methods A prospective study was conducted over a 27-month period. Hepatic, VAT, and SAT samples were obtained at the time of surgery. 11β-HSD1 and 18S gene expression was measured using real-time quantitative reverse transcriptase-polymerase chain reaction. Results Forty nine patients met the inclusion criteria [mean age: 42.2 ± 10 years, body mass index (BMI): 42 ± 6 kg/m2, 71% women and 63% with metabolic syndrome (MS)]. 11β-HSD1 mRNA levels were higher in liver than fat tissue (p < 0.001), being higher in SAT than in VAT (p < 0.001) without gender-specific differences. Hepatic expression of 11β-HSD1 correlated positively with SAT and VAT, alanine aminotransferase (ALT), and serum glucose and was inversely associated with BMI. 11β-HSD1 mRNA in VAT correlated positively with insulinemia, ALT, and LDL cholesterol. There were no associations between 11β-HSD1 mRNA in SAT and the variables analyzed. Conclusions 11β-HSD1 expression is higher in liver in comparison to adipose tissue in obese patients. The observed correlations between hepatic and VAT 11β-HSD1 expression with dyslipidemia and insulin resistance suggest that this enzyme might have a pathogenic role in obesity and related metabolic disorders.
Research Interests: Bariatric Surgery, Obesity, Gene expression, Metabolic syndrome, Insulin Resistance, and 35 morereal time PCR, Adipose tissue, Cortisol, Humans, Liver, Mice, Cholesterol, Animals, Corticosterone, Young Adult, non alcoholic fatty liver disease (NAFLD), Body Mass Index, ROC Curve, Obesity Surgery, Triglycerides, LDL-cholesterol, Enzyme, Clinical Sciences, Fatty Liver, Fat Mass, Body mass index (BMI), Public health systems and services research, Alanine Aminotransferase, Odds ratio, Western blot, Protein Expression, Metabolic Disorder, Abdominal Obesity, Fasting Insulin, Enzyme Linked Immunosorbent Assay, Glucocorticoids, Prospective Study, Cushing Syndrome, Gene expression profiling, and Alanine Transaminase
Research Interests:
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic... more
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic disorders associated with obesity. The aim of this study was to evaluate the 11β-HSD1 expression in liver, visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in morbidly obese patients undergoing bariatric surgery and its correlations with clinical, anthropometric, and biochemical variables. Methods A prospective study was conducted over a 27-month period. Hepatic, VAT, and SAT samples were obtained at the time of surgery. 11β-HSD1 and 18S gene expression was measured using real-time quantitative reverse transcriptase-polymerase chain reaction. Results Forty nine patients met the inclusion criteria [mean age: 42.2 ± 10 years, body mass index (BMI): 42 ± 6 kg/m2, 71% women and 63% with metabolic syndrome (MS)]. 11β-HSD1 mRNA levels were higher in liver than fat tissue (p < 0.001), being higher in SAT than in VAT (p < 0.001) without gender-specific differences. Hepatic expression of 11β-HSD1 correlated positively with SAT and VAT, alanine aminotransferase (ALT), and serum glucose and was inversely associated with BMI. 11β-HSD1 mRNA in VAT correlated positively with insulinemia, ALT, and LDL cholesterol. There were no associations between 11β-HSD1 mRNA in SAT and the variables analyzed. Conclusions 11β-HSD1 expression is higher in liver in comparison to adipose tissue in obese patients. The observed correlations between hepatic and VAT 11β-HSD1 expression with dyslipidemia and insulin resistance suggest that this enzyme might have a pathogenic role in obesity and related metabolic disorders.
Research Interests: Bariatric Surgery, Obesity, Gene expression, Metabolic syndrome, Insulin Resistance, and 35 morereal time PCR, Adipose tissue, Cortisol, Humans, Liver, Mice, Cholesterol, Animals, Corticosterone, Young Adult, non alcoholic fatty liver disease (NAFLD), Body Mass Index, ROC Curve, Obesity Surgery, Triglycerides, LDL-cholesterol, Enzyme, Clinical Sciences, Fatty Liver, Fat Mass, Body mass index (BMI), Public health systems and services research, Alanine Aminotransferase, Odds ratio, Western blot, Protein Expression, Metabolic Disorder, Abdominal Obesity, Fasting Insulin, Enzyme Linked Immunosorbent Assay, Glucocorticoids, Prospective Study, Cushing Syndrome, Gene expression profiling, and Alanine Transaminase
Research Interests:
Research Interests: Bariatric Surgery, Metabolism, Lipids, Cortisol, Humans, and 21 moreBlood Glucose, Insulin, Liver, Female, Male, Body Mass Index, Enzyme, Clinical Sciences, Aged, Middle Aged, C reactive protein, Adiponectin, Adult, Body Mass, Central Obesity, Biological markers, Adaptive Response, Metabolic Disorder, Aspartate Aminotransferases, Cushing Syndrome, and Alanine Transaminase
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic... more
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic disorders associated with obesity. The aim of this study was to evaluate the 11β-HSD1 expression in liver, visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in morbidly obese patients undergoing bariatric surgery and its correlations with clinical, anthropometric, and biochemical variables. Methods A prospective study was conducted over a 27-month period. Hepatic, VAT, and SAT samples were obtained at the time of surgery. 11β-HSD1 and 18S gene expression was measured using real-time quantitative reverse transcriptase-polymerase chain reaction. Results Forty nine patients met the inclusion criteria [mean age: 42.2 ± 10 years, body mass index (BMI): 42 ± 6 kg/m2, 71% women and 63% with metabolic syndrome (MS)]. 11β-HSD1 mRNA levels were higher in liver than fat tissue (p < 0.001), being higher in SAT than in VAT (p < 0.001) without gender-specific differences. Hepatic expression of 11β-HSD1 correlated positively with SAT and VAT, alanine aminotransferase (ALT), and serum glucose and was inversely associated with BMI. 11β-HSD1 mRNA in VAT correlated positively with insulinemia, ALT, and LDL cholesterol. There were no associations between 11β-HSD1 mRNA in SAT and the variables analyzed. Conclusions 11β-HSD1 expression is higher in liver in comparison to adipose tissue in obese patients. The observed correlations between hepatic and VAT 11β-HSD1 expression with dyslipidemia and insulin resistance suggest that this enzyme might have a pathogenic role in obesity and related metabolic disorders.
Research Interests: Bariatric Surgery, Obesity, Gene expression, Metabolic syndrome, Insulin Resistance, and 35 morereal time PCR, Adipose tissue, Cortisol, Humans, Liver, Mice, Cholesterol, Animals, Corticosterone, Young Adult, non alcoholic fatty liver disease (NAFLD), Body Mass Index, ROC Curve, Obesity Surgery, Triglycerides, LDL-cholesterol, Enzyme, Clinical Sciences, Fatty Liver, Fat Mass, Body mass index (BMI), Public health systems and services research, Alanine Aminotransferase, Odds ratio, Western blot, Protein Expression, Metabolic Disorder, Abdominal Obesity, Fasting Insulin, Enzyme Linked Immunosorbent Assay, Glucocorticoids, Prospective Study, Cushing Syndrome, Gene expression profiling, and Alanine Transaminase
Research Interests: Bariatric Surgery, Metabolism, Lipids, Cortisol, Humans, and 21 moreBlood Glucose, Insulin, Liver, Female, Male, Body Mass Index, Enzyme, Clinical Sciences, Aged, Middle Aged, C reactive protein, Adiponectin, Adult, Body Mass, Central Obesity, Biological markers, Adaptive Response, Metabolic Disorder, Aspartate Aminotransferases, Cushing Syndrome, and Alanine Transaminase
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic... more
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic disorders associated with obesity. The aim of this study was to evaluate the 11β-HSD1 expression in liver, visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in morbidly obese patients undergoing bariatric surgery and its correlations with clinical, anthropometric, and biochemical variables. Methods A prospective study was conducted over a 27-month period. Hepatic, VAT, and SAT samples were obtained at the time of surgery. 11β-HSD1 and 18S gene expression was measured using real-time quantitative reverse transcriptase-polymerase chain reaction. Results Forty nine patients met the inclusion criteria [mean age: 42.2 ± 10 years, body mass index (BMI): 42 ± 6 kg/m2, 71% women and 63% with metabolic syndrome (MS)]. 11β-HSD1 mRNA levels were higher in liver than fat tissue (p < 0.001), being higher in SAT than in VAT (p < 0.001) without gender-specific differences. Hepatic expression of 11β-HSD1 correlated positively with SAT and VAT, alanine aminotransferase (ALT), and serum glucose and was inversely associated with BMI. 11β-HSD1 mRNA in VAT correlated positively with insulinemia, ALT, and LDL cholesterol. There were no associations between 11β-HSD1 mRNA in SAT and the variables analyzed. Conclusions 11β-HSD1 expression is higher in liver in comparison to adipose tissue in obese patients. The observed correlations between hepatic and VAT 11β-HSD1 expression with dyslipidemia and insulin resistance suggest that this enzyme might have a pathogenic role in obesity and related metabolic disorders.
Research Interests: Bariatric Surgery, Obesity, Gene expression, Metabolic syndrome, Insulin Resistance, and 35 morereal time PCR, Adipose tissue, Cortisol, Humans, Liver, Mice, Cholesterol, Animals, Corticosterone, Young Adult, non alcoholic fatty liver disease (NAFLD), Body Mass Index, ROC Curve, Obesity Surgery, Triglycerides, LDL-cholesterol, Enzyme, Clinical Sciences, Fatty Liver, Fat Mass, Body mass index (BMI), Public health systems and services research, Alanine Aminotransferase, Odds ratio, Western blot, Protein Expression, Metabolic Disorder, Abdominal Obesity, Fasting Insulin, Enzyme Linked Immunosorbent Assay, Glucocorticoids, Prospective Study, Cushing Syndrome, Gene expression profiling, and Alanine Transaminase
Research Interests: Bariatric Surgery, Metabolism, Lipids, Cortisol, Humans, and 21 moreBlood Glucose, Insulin, Liver, Female, Male, Body Mass Index, Enzyme, Clinical Sciences, Aged, Middle Aged, C reactive protein, Adiponectin, Adult, Body Mass, Central Obesity, Biological markers, Adaptive Response, Metabolic Disorder, Aspartate Aminotransferases, Cushing Syndrome, and Alanine Transaminase
Research Interests: Bariatric Surgery, Metabolism, Lipids, Cortisol, Humans, and 21 moreBlood Glucose, Insulin, Liver, Female, Male, Body Mass Index, Enzyme, Clinical Sciences, Aged, Middle Aged, C reactive protein, Adiponectin, Adult, Body Mass, Central Obesity, Biological markers, Adaptive Response, Metabolic Disorder, Aspartate Aminotransferases, Cushing Syndrome, and Alanine Transaminase
Research Interests:
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic... more
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic disorders associated with obesity. The aim of this study was to evaluate the 11β-HSD1 expression in liver, visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in morbidly obese patients undergoing bariatric surgery and its correlations with clinical, anthropometric, and biochemical variables. Methods A prospective study was conducted over a 27-month period. Hepatic, VAT, and SAT samples were obtained at the time of surgery. 11β-HSD1 and 18S gene expression was measured using real-time quantitative reverse transcriptase-polymerase chain reaction. Results Forty nine patients met the inclusion criteria [mean age: 42.2 ± 10 years, body mass index (BMI): 42 ± 6 kg/m2, 71% women and 63% with metabolic syndrome (MS)]. 11β-HSD1 mRNA levels were higher in liver than fat tissue (p < 0.001), being higher in SAT than in VAT (p < 0.001) without gender-specific differences. Hepatic expression of 11β-HSD1 correlated positively with SAT and VAT, alanine aminotransferase (ALT), and serum glucose and was inversely associated with BMI. 11β-HSD1 mRNA in VAT correlated positively with insulinemia, ALT, and LDL cholesterol. There were no associations between 11β-HSD1 mRNA in SAT and the variables analyzed. Conclusions 11β-HSD1 expression is higher in liver in comparison to adipose tissue in obese patients. The observed correlations between hepatic and VAT 11β-HSD1 expression with dyslipidemia and insulin resistance suggest that this enzyme might have a pathogenic role in obesity and related metabolic disorders.
Research Interests: Bariatric Surgery, Obesity, Gene expression, Metabolic syndrome, Insulin Resistance, and 35 morereal time PCR, Adipose tissue, Cortisol, Humans, Liver, Mice, Cholesterol, Animals, Corticosterone, Young Adult, non alcoholic fatty liver disease (NAFLD), Body Mass Index, ROC Curve, Obesity Surgery, Triglycerides, LDL-cholesterol, Enzyme, Clinical Sciences, Fatty Liver, Fat Mass, Body mass index (BMI), Public health systems and services research, Alanine Aminotransferase, Odds ratio, Western blot, Protein Expression, Metabolic Disorder, Abdominal Obesity, Fasting Insulin, Enzyme Linked Immunosorbent Assay, Glucocorticoids, Prospective Study, Cushing Syndrome, Gene expression profiling, and Alanine Transaminase
Research Interests: Bariatric Surgery, Metabolism, Lipids, Cortisol, Humans, and 21 moreBlood Glucose, Insulin, Liver, Female, Male, Body Mass Index, Enzyme, Clinical Sciences, Aged, Middle Aged, C reactive protein, Adiponectin, Adult, Body Mass, Central Obesity, Biological markers, Adaptive Response, Metabolic Disorder, Aspartate Aminotransferases, Cushing Syndrome, and Alanine Transaminase
Research Interests:
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic... more
Background The enzyme 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes intracellular glucocorticoid reactivation by conversion of cortisone to cortisol in different tissues and have been implicated in several metabolic disorders associated with obesity. The aim of this study was to evaluate the 11β-HSD1 expression in liver, visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) in morbidly obese patients undergoing bariatric surgery and its correlations with clinical, anthropometric, and biochemical variables. Methods A prospective study was conducted over a 27-month period. Hepatic, VAT, and SAT samples were obtained at the time of surgery. 11β-HSD1 and 18S gene expression was measured using real-time quantitative reverse transcriptase-polymerase chain reaction. Results Forty nine patients met the inclusion criteria [mean age: 42.2 ± 10 years, body mass index (BMI): 42 ± 6 kg/m2, 71% women and 63% with metabolic syndrome (MS)]. 11β-HSD1 mRNA levels were higher in liver than fat tissue (p < 0.001), being higher in SAT than in VAT (p < 0.001) without gender-specific differences. Hepatic expression of 11β-HSD1 correlated positively with SAT and VAT, alanine aminotransferase (ALT), and serum glucose and was inversely associated with BMI. 11β-HSD1 mRNA in VAT correlated positively with insulinemia, ALT, and LDL cholesterol. There were no associations between 11β-HSD1 mRNA in SAT and the variables analyzed. Conclusions 11β-HSD1 expression is higher in liver in comparison to adipose tissue in obese patients. The observed correlations between hepatic and VAT 11β-HSD1 expression with dyslipidemia and insulin resistance suggest that this enzyme might have a pathogenic role in obesity and related metabolic disorders.