Background: Pediatric patients on chemotherapy are vulnerable to invasive fungal infection especially Candida spices. Resistance to antifungal agents has increased in Candida spp., especially in non-albicans species. This study aims to... more
Background: Pediatric patients on chemotherapy are vulnerable to invasive fungal infection especially Candida spices. Resistance to antifungal agents has increased in Candida spp., especially in non-albicans species. This study aims to assess the susceptibility of Candida spp. strains isolated from children with acute leukemia less than 18 years. Materials and Methods: This prospective cross-sectional study was conducted during March 2011 to March 2012. Participants were 188 children aging from 1 month to 18 years, who had acute leukemia, were admitted in Amir Oncology Hospital affiliated to Shiraz University of Medical Science, Shiraz, Iran. Identification of Candida strains was performed using germ tube and chlamydospore production tests on an Application Programming Interface (API) 20 C AUX yeast identification system. Susceptibility testing for 7 antifungal agents was performed by the agar-based E-test method. Fungal cultures were carried out from nose, oropharynx, stool, and ur...
Research Interests:
Most of the microspore-derived embryos can not regenerate normally in rapeseed. The effects of gibberellins (GA3), abscisic acid (ABA), and embryo desiccation on normal plantlet regeneration were studied. The donor plants were grown in a... more
Most of the microspore-derived embryos can not regenerate normally in rapeseed. The effects of gibberellins (GA3), abscisic acid (ABA), and embryo desiccation on normal plantlet regeneration were studied. The donor plants were grown in a growth chamber at day/night temperatures of 15/10˚C with a 16/8h photoperiod, respectively. Microspores were isolated from whole buds of 2.5-3.5mm in length, containing late-uninucleate and early-binucleate microspores, and cultured in modified NLN13 liquid medium. After 30 days, cotyledonary embryos were transferred to B5 medium. The study of GA3 concentrations (0, 0.05, 0.1, 0.15, 0.2 mg/l) showed that the use of 0.1 and 0.15 mg/l of filter sterilized GA3 were the optimum treatment for normal plantlet production (50% and 44%, respectively). Among the various time periods of embryos desiccation (0, 3, 5, 10, 15, 20 min.), air drying of embryos for 10 min. produced the highest normal plantlets (60%). In the third experiment, 9 desiccation-ABA treatm...
Research Interests:
Research Interests:
... The regions within genomes that contain genes asso-ciated with particular quantitative trait are recognized as quantitative trait loci (QTLs). QTLs controlling important traits such as in vitro regeneration parameters (Flores Berrios... more
... The regions within genomes that contain genes asso-ciated with particular quantitative trait are recognized as quantitative trait loci (QTLs). QTLs controlling important traits such as in vitro regeneration parameters (Flores Berrios et al. ...
Research Interests:
Research Interests:
Systemic candidiasis, are common infections during the neutropenic phase. The aim of this study was to identify quantitative Candida species ribosomal DNA using TaqMan technology for diagnosing candidiasis and monitoring them during... more
Systemic candidiasis, are common infections during the neutropenic phase. The aim of this study was to identify quantitative Candida species ribosomal DNA using TaqMan technology for diagnosing candidiasis and monitoring them during hospitalization. During the prospective, cross-sectional study, from September 2006 to September 2007, a total of 375 clinical blood specimens were collected from 35 patients with hematologic disorders once a week pretransplant and posttransplant. Patients were evaluated for systemic candidiasis during hospitalization. Cultures from the throat, urine, feces, and sputum, along with sonography and computerized tomographic scans, were done when patients were febrile and not having a response to antibiotics. All samples were cultured on Sabouraud dextrose agar with chloramphenicol, and direct, microscopic examination was performed. Blood samples were cultured by bedside inoculation into BACTEC medium at 35 degrees C for 7 days. Clinical blood specimens were ...
Research Interests: Adolescent, Medicine, Iran, Prospective studies, Humans, and 15 moreChild, Female, Candida, Male, Candidiasis, Polymerase Chain Reaction, Middle Aged, Bone Marrow Transplantation, Adult, Sputum, Cross Sectional Studies, Early Diagnosis, Predictive value of tests, Child preschool, and Immunosuppressive Agents
Systemic candidiasis is a significant cause of morbidity and mortality in patients with hematologic disorders. The aim of this study was to determine the prevalence of systemic candidiasis and the efficiency of the polymerase chain... more
Systemic candidiasis is a significant cause of morbidity and mortality in patients with hematologic disorders. The aim of this study was to determine the prevalence of systemic candidiasis and the efficiency of the polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELIZA) method for the early detection of Candida spp. in patients with hematologic malignancies. From 2004 to 2006, 194 patients with hematologic malignancies were evaluated for systemic candidiasis. Collected blood samples were assayed using the PCR-ELISA method for the presence of the bands on ethidium bromide stained gel, and for hybridization with Candida spp. as well. The female-to-male ratio was 61:133, the mean age was 33.7 years, and the mean hospitalization period was 21.2 days. Twenty-five patients (12.9%) had positive PCR-ELISA results for systemic candidiasis. The etiologic agents were Candida albicans (21 cases), C. tropicalis (3 cases), and C. krusei (1 case). The mean interval of PCR-ELISA pos...
Research Interests: Biology, Adolescent, Medicine, Humans, Female, and 15 moreBlood sampling, Candida, Male, Candidiasis, Polymerase Chain Reaction, Candida albicans, Length of Stay, Middle Aged, Early Detection, Adult, Antifungal Agents, Clinical Signs, Enzyme Linked Immunosorbent Assay, Ethidium bromide, and Hematologic neoplasms
Research Interests:
Introduction: Limited specific data and investigations are available for invasive aspergillosis (IA) in pediatric patients. We evaluated the diagnostic potential of three noninvasive tests including the Platelia Aspergillus EIA kit for... more
Introduction: Limited specific data and investigations are available for invasive aspergillosis (IA) in pediatric patients. We evaluated the diagnostic potential of three noninvasive tests including the Platelia Aspergillus EIA kit for using galactomannan antigen, (1,3)-β-D-glucan Detection Reagent Kit, and nested-PCR for Aspergillus DNA in sera. We evaluated the diagnostic potential of three noninvasive tests including EIA for galactomannan antigen (Platelia Aspergillus), nested PCR assay for Aspergillus DNA and test for (1→3)-β-D-glucan (Glucatell assay Kit). Methodology: All pediatric patients treated at the hematology/oncology unit who were at increased risk of developing invasive aspergillosis were enrolled. Clinical samples were examined for Aspergillus infections by mycological methods. Serial blood samples were collected twice weekly and evaluated by noninvasive tests. Results: We analyzed 230 consecutive blood samples from 62 pediatric patients. The incidence rate of inv...
Research Interests:
Research Interests: Genetics, Plant Biology, Biology, Molecular Breeding, QTL mapping, and 12 moreMolecular, Chronic Disease, Genetic linkage analysis, Sunflower, Linkage Group, Phenotypic variation, Quantitative Trait Loci, Helianthus Annuus, Helianthus annuus L, Simple Sequence Repeat, QTL analysis, and Genetic control
Research Interests:
Research Interests:
Research Interests:
Orbital mucormycosis caused by Zygomycetes is a rare and fatal infection that generally affects the patients who are immunocompromised. Despite antifungal therapy and aggressive surgical intervention, mucormycosis can cause serious and... more
Orbital mucormycosis caused by Zygomycetes is a rare and fatal infection that generally affects the patients who are immunocompromised. Despite antifungal therapy and aggressive surgical intervention, mucormycosis can cause serious and rapidly fatal infections if delayed diagnosis or therapeutic management occurs. Here, we report orbital mucormycosis in a healthy boy, with a favorable outcome after aggressive treatment. He has had no recurrence since the end of his treatment. A 2-year old healthy boy, some days after entry of dust particle to his left eye presented with swelling and redness of the eye. With diagnosis of "periorbital cellulitis" intravenous antibiotics vancomycin (40 mg/kg/day) and ceftriaxone (75 mg/kg/day) were started but no improvement was observed. The results of biopsy and tissue culture led us to a diagnosis of mucormycosis. Orbital exenteration, combined with intravenous amphotericin B (1 mg/kg/day), resulted in the patient's survival. Due to th...