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... (type IV) Arvydas Janulaitis*, Maryte Petrusyte, Zita Maneliene, Saulius Klimasauskas+ and Viktoras Butkus Institute of Biotechnology FERMENTAS, Graiciuno 8, 232028 Vilnius, Lithuania Received July 6, 1992; Revised and Accepted... more
... (type IV) Arvydas Janulaitis*, Maryte Petrusyte, Zita Maneliene, Saulius Klimasauskas+ and Viktoras Butkus Institute of Biotechnology FERMENTAS, Graiciuno 8, 232028 Vilnius, Lithuania Received July 6, 1992; Revised and Accepted October 26, 1992 ...
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Two additional conserved motifs (CM), CM Is and CM III, have been found in addition to well-known CM I and CM II within the primary amino acid sequences of almost all m6A- and m4C-methyltransferases (MTases). The boundaries of all four CM... more
Two additional conserved motifs (CM), CM Is and CM III, have been found in addition to well-known CM I and CM II within the primary amino acid sequences of almost all m6A- and m4C-methyltransferases (MTases). The boundaries of all four CM were defined and their consensus sequences characteristic both for different classes, as well as for all N-MTases, were derived. Some regular deviations at fixed positions of the consensus sequences CM Is, CM I and CM II, typical for separate classes of N-MTases, were presumed to correlate. A possible structural basis for the supposed interregional correlations is discussed and experiments for verification of the assumed interactions between CM are suggested. A classification scheme for all N-MTases is provided.
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The intrinsic insensitivity of EcoRII recognition sites in RF DNAs of phage M13 and vector M13mp18 towards this restriction endonuclease can be overcome by adding site-specific oligodeoxyribonucleotide duplexes to the restriction sample.... more
The intrinsic insensitivity of EcoRII recognition sites in RF DNAs of phage M13 and vector M13mp18 towards this restriction endonuclease can be overcome by adding site-specific oligodeoxyribonucleotide duplexes to the restriction sample. Since Dcm- DNA but not Dcm(+)-methylated DNA becomes susceptible under these conditions, this procedure constitutes an improvement of the Dcm methylation assay.