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Micromorphological attributes for five species of C. gynandra L., C. viscosa L, C. scaposa DC, C. paradoxa B.BR and C. brachycarpa Vahl ex DC. belonging to genus Cleome L. in south west of Saudi Arabia was studied by light (LM) and... more
Micromorphological attributes for five species of C. gynandra L., C. viscosa L, C. scaposa DC, C. paradoxa B.BR and C. brachycarpa Vahl ex DC. belonging to genus Cleome L. in south west of Saudi Arabia was studied by light (LM) and scanning electron microscope (SEM). Studies are interested by hair characters, stomata behaviour, stem and leaf anatomy, pollen grains and seed exomorphic attributes. Stem and leaf anatomy differentiation indicated good taxonomic tools to between species the species, the result of the stomata frequency was significant where the highest stomatal index (SI) of 36.4% was observed in C. gynandra while the lowest one (21.6%) was noticed in C. Paradoxa. However, pollen grains and seed exomorphic is significantly helpful at the specific level. Prolate, tricolpate, monad pollen grains are found in all studied species pollen grains differed in P/E (polar axis / equatorial axis) ratio and exine measurements. Also, every Cleome species has their own seed ornamentati...
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These studies were conducted on the twoflavonoid compounds of quercetin and kaempferol in addition electrophoretic studies of esterase and peroxidase isoenzymes by SDS-polyacrylamide gel electrophoresisfor five CleomeL. species.... more
These studies were conducted on the twoflavonoid compounds of quercetin and kaempferol in addition electrophoretic studies of esterase and peroxidase isoenzymes by SDS-polyacrylamide gel electrophoresisfor five CleomeL. species. Extraction of these flavonoids was carried out using 90% ethanol and 85% methanol. Identification and quantification of quercetin and kaempferol was done using thin Layer chromatography (TLC) and further augmented using high performance liquid chromatography (HPLC) and then isolated,purified and identified using melting point (M.P.) and infrared spectroscopy (IR).eightquercetinand seven kaempferol compounds are isolated and identified with different percentages. Electrophoretic patterns of two isoenzymes ofesterases (Est) and peroxidases (Prx) considered a good taxonomicmarker techniques. A total 45 esterase and peroxidase bands obtained scanning the gels. The highest combined esterase and peroxidase bands (13 bands) are found in C.scaposa DCwhereasthe l...
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The present studies are interested the chemotaxonomy including the phytochemical and molecular screening of the 11 cultivars of Coffea arabica obtained from Jazan, KSA. Total alkaloids, terpenoids, flavonoids, saponins were assessed and... more
The present studies are interested the chemotaxonomy including the phytochemical and molecular screening of the 11 cultivars of Coffea arabica obtained from Jazan, KSA. Total alkaloids, terpenoids, flavonoids, saponins were assessed and compared. Alkaloids and flavonoids are assayed by the method of thin layer chromatography (TLC). 14 phenolic compounds assayed by high-performance liquid chromatography (HPLC) method. Seed protein diversity as reveled by variation in SDS-PAGE techniques been used as molecular methods. Statistical analysis of the phytochemical and the molecular data are analyzed separately and grouped using Minitab 17.0 statistical software. obtained statistical data resulted in two groups, the first includes cv. jazani, cv. Yemeni, cv. Chinese, cv. Colombian, cv. Habshi, cv. Yafi, cv. Indian, cv. Kenyan and cv. Brutte. The second group included the two cultivars of Ethiobian Harar and Brazilian. In the first group, three subgroups are recognized, cv. Yafi, cv. Yameni...
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The present study provides an analysis of quantitative vegetation gradient and the edaphic variables in Tihamah hill slopes of Jazan region. A total of 68 species belonging to 31 families of the vascular plants were recorded from 17... more
The present study provides an analysis of quantitative vegetation gradient and the edaphic variables in Tihamah hill slopes of Jazan region. A total of 68 species belonging to 31 families of the vascular plants were recorded from 17 sites. Phanerophyte and chamaephytes were most frequent, denoting a typical desert life form. Classification of the vegetation analyzed using two-way indicator species analysis (TWINSPAN) techniques, resulted in estimation of five major community types: Acacia ehrenbergiana Haynem, Anisotes triculcus (Forssk.) DC, Salvadora persica L and Ziziphus spina-christi (L.) Willd communities; Salvadora persica L. Ziziphus spina-christi (L.) Willd and Acacia asak (Forssk.) Willd communities; Acacia ehrenbergiana Haynem, Aerva javanica (Burm.f.) Juss and Indigofera spinosa Forssk communities; Dactyloctenium scindicum Boiss, Schweinfurthia pterosperma Rich and Acacia tortilis (Forssk.) Hayne communities and Tamarix nilotica (Ehrenb) Bunge and Dobera glabra (Forssk.)...
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Seed protein diversity as revealed by variation in SDS-PAGE has been used to reassess the taxonomic relationships between 35 species related to 24 gemera of the Brassicaceae. A total of number of 82 protein bands with molecular weight... more
Seed protein diversity as revealed by variation in SDS-PAGE has been used to reassess the taxonomic relationships between 35 species related to 24 gemera of the Brassicaceae. A total of number of 82 protein bands with molecular weight ranging between 0.064 and 0.94 were recorded in the electropherograms. These bands were used as binary characters and analyzed by the STATISCA program package using the UPGMA clustering method. The produced dendrogram from SDS-PAGE analysis showed separation of the two species of genus Raphanus at taxonomoc level of 62% wherase the remainder are separated into two groups. Also, the dendrogram showed a close affinity between genus Lepidium and genus Capsella. The phenogram shows that eight species of genus Brassica from different geographical locations are not grouped together in one cluster.
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The morphological and anatomical characters of two wild Tephrosia species grown in Aden were investigated .The leaf; the shape, apex, margin, base, texture, size and venation of lamina were studied . The epidermal characters including the... more
The morphological and anatomical characters of two wild Tephrosia species grown in Aden were investigated .The leaf; the shape, apex, margin, base, texture, size and venation of lamina were studied . The epidermal characters including the properties of epidermal cells (Shape, size & frequency), stomata properties (Type of stomata complex, size, frequency, index and ratio) stomata properties of (Adaxial & Abaxial) and type and size of trichomes and the anatomical characters (Thickness of Mesophyll and number of abaxial collenchyma layers in the midrib) and type of trichomes were determined. The stem and petiole characters and main midrib were investigated. The macro and micromorphological characters show a high significant in taxonomic value for the separation between the two studied Tephrosia species. They were 4 species in 1992 and in this study only 2 were found, that due to the human influence and heavily urban raise at every habitat in Aden.
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Twenty-one species belonging to 13 genera of Acanthaceae obtained from Saudi Arabia were studied using electrophoretic analysis for protein patterns and two isoenzymes polymorphism in addition the phytochemical screening by TLC and HPLC.... more
Twenty-one species belonging to 13 genera of Acanthaceae obtained from Saudi Arabia were studied using electrophoretic analysis for protein patterns and two isoenzymes polymorphism in addition the phytochemical screening by TLC and HPLC. A total number of twenty-three protein bands observed between the species ranged 10 and 250 K Da, nine esterase and six peroxidase polymorphisms were detected. The biochemical data (protein profiles and isoenzymes polymorphisms) considered a significant tools for the relationships between the studied species. Furthermore, the phytochemical screening including the total contents of alkaloids, flavonoids, terpenoids and saponins were demonstrated the presence of alkaloids, flavonoids in all studied taxa with a variable contents and absence of total terpenoides and saponins in some species. As well, fourteen phenolic acids have been screened by high-performance liquid chromatography (HPLC) method. Gallic acid, phenol, protocatechuic acid, p-coumaric ac...