Abstract Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening... more
Abstract Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the~ 21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes.
Abstract Arginine (R)-based ER localization signals are sorting motifs that confer transient ER localization to unassembled subunits of multimeric membrane proteins. The COPI vesicle coat binds R-based signals but the molecular details... more
Abstract Arginine (R)-based ER localization signals are sorting motifs that confer transient ER localization to unassembled subunits of multimeric membrane proteins. The COPI vesicle coat binds R-based signals but the molecular details remain unknown. Here, we use reporter membrane proteins based on the proteolipid Pmp2 fused to GFP and allele swapping of COPI subunits to map the recognition site for R-based signals.
Abstract Zebrafish were established as model organisms for biological studies of embryonic development and toxicology testing. Automated manipulation and microscopy of living organisms is needed in all process steps and several new robots... more
Abstract Zebrafish were established as model organisms for biological studies of embryonic development and toxicology testing. Automated manipulation and microscopy of living organisms is needed in all process steps and several new robots have been developed in the last years. The impact of abiotic stress factors like low and high ambient temperatures on zebrafish embryo development have been quantified thoroughly in the past.
Eine rein schlagwortbasierte Suche stößt hier auf die bekannten Probleme: 1. Die Qualität der Suchergebnisse hängt stark von einer geschickten Komposition der Suchbegriffe ab. Oft werden viele irrelevante Dokumente gefunden, die die... more
Eine rein schlagwortbasierte Suche stößt hier auf die bekannten Probleme: 1. Die Qualität der Suchergebnisse hängt stark von einer geschickten Komposition der Suchbegriffe ab. Oft werden viele irrelevante Dokumente gefunden, die die jeweiligen Schlagwörter enthalten. Hingegen werden relevante Dokumente selbst bei geringfügig abweichenden Begriffen (zB Synonymen) nicht gefunden.
In den letzten Jahren etablierten sich Larven des Zebrabärblings danio rerio als Modellorganismus für das Verständnis der Embryonalentwicklung und von Zellbiologischen Prozessen. Außerdem werden Zebrafischlarven zunehmend als Alternative... more
In den letzten Jahren etablierten sich Larven des Zebrabärblings danio rerio als Modellorganismus für das Verständnis der Embryonalentwicklung und von Zellbiologischen Prozessen. Außerdem werden Zebrafischlarven zunehmend als Alternative zu Tierversuchen an Säugetieren für Toxizitäts-Bestimmungen eingesetzt [1, 2].
Figure 1. Implementation of algorithms as a graphical user interface.(A) Screenshot of the graphical user interface for the execution of automatic and manual ROI detection, coordinate transformation, and XML batch job file generation.
Table S2). However, gfp RNAi did not show any dilution effect, consistent with the experiment described above. These data suggest that RNAi of some genes, but not all, showed the dilution effect and that using a single vector expressing... more
Table S2). However, gfp RNAi did not show any dilution effect, consistent with the experiment described above. These data suggest that RNAi of some genes, but not all, showed the dilution effect and that using a single vector expressing two dsRNA species is a way to effectively avoid dilution and achieve RNAi effects. To examine whether the new method is also applicable to endogenous genes, we tested the RNAi effects of the dpy-5 and/or mes-3 genes. The dpy-5 gene en codes cuticle collagen.
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Publikationsansicht. 31352522. Entwicklung und Anwendungen einer modularen High-content-screening-Plattform zur funktionellen und bioinformatischen Genomanalyse / (2004).Liebel, Urban. Abstract. Enth. ausserdem 1 Sonderabdr. aus: FEBS... more
Publikationsansicht. 31352522. Entwicklung und Anwendungen einer modularen High-content-screening-Plattform zur funktionellen und bioinformatischen Genomanalyse / (2004).Liebel, Urban. Abstract. Enth. ausserdem 1 Sonderabdr. aus: FEBS letters ; Vol. 554. ...
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Research Interests: Toxicology, Survival Analysis, Hydrobiology, Danio rerio, High throughput screening, and 13 moreAnimals, Genetically modified organisms, Laboratory, Cost Efficiency, Survival Rate, Reproducibility of Results, Zebrafish, Toxicity Tests, Laboratory Automation, Cell Sorting, Toxicity Testing, Egg Size, and ovum
In this paper, an automated process to extract experiment-specific parameters out of microscope images of zebrafish embryos is presented and applied to experiments consisting of toxicological treated zebrafish embryos. The treatments... more
In this paper, an automated process to extract experiment-specific parameters out of microscope images of zebrafish embryos is presented and applied to experiments consisting of toxicological treated zebrafish embryos. The treatments consist of a dilution series of several compounds. A custom built graphical user interface allows an easy labeling and browsing of the image data. Subsequently image-specific features are extracted for each image based on image processing algorithms. By means of feature selection, the ...
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Research Interests: Inflammation, Innate immunity, High Content Screening, Biological Sciences, Reactive Oxygen Species, and 15 moreHigh throughput screening, Animals, Animal Model, Genetic Screening, Anti-inflammatory agents, Real Time, Fish Larvae, Model System, Very high throughput, Fluorescent Protein, Zebrafish, Leukocytes, Copper sulfate, Lateral Line, and Inflammatory response
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Abstract Harvester is a meta search engine for gene and protein information. It searches 16 major databases and prediction servers and combines the results on pregenerated HTML pages. In this way Harvester can provide comprehensive... more
Abstract Harvester is a meta search engine for gene and protein information. It searches 16 major databases and prediction servers and combines the results on pregenerated HTML pages. In this way Harvester can provide comprehensive geneprotein information from ...