- Lincoln, New Zealand
Victoria Metcalf
Lincoln University NZ, Wine, Food & Molecular Bioscience, Faculty Member
- Molecular Biology, Biochemistry, Evolutionary Biology, Genetics, Aquaculture, Antarctica, and 19 moreEcology, Polar Studies, Climate Change, Climate Change Adaptation, Ocean acidification, Marine Biology, Ocean Acidification and Its Impact on Marine Calcifying Organisms, Science Communication, Fish Biology, Shellfish Ecology and Fisheries, Evolution, Genomics, Fisheries, Chondrichthyes, Fatty Acid, Albumin, Lipid, Elasmobranch, and Protein Sequence Analysisedit
Research Interests:
Homeoviscous adaptation (HVA) is a key cellular response by which fish protect their membranes against thermal stress. We investigated evolutionary HVA (long time scale) in Antarctic and non-Antarctic fish. Membrane lipid composition was... more
Homeoviscous adaptation (HVA) is a key cellular response by which fish protect their membranes against thermal stress. We investigated evolutionary HVA (long time scale) in Antarctic and non-Antarctic fish. Membrane lipid composition was determined for four Perciformes fish: two closely related Antarctic notothenioid species ( and ); a diversified related notothenioid Antarctic icefish (); and a New Zealand species (). The membrane lipid compositions were consistent across the three Antarctic species and these were significantly different from that of the New Zealand species. Furthermore, acclimatory HVA (short time periods with seasonal changes) was investigated to determine whether stenothermal Antarctic fish, which evolved in the cold, stable environment of the Southern Ocean, have lost the acclimatory capacity to modulate their membrane saturation states, making them vulnerable to anthropogenic global warming. We compared liver membrane lipid composition in two closely related A...
Research Interests:
The phylogenetic relationships of the two extant lobe-finned fish, coelacanth and lungfish, to tetrapods remain unresolved. In our study of serum albumin in lungfish we determined 101 residues of protein sequence from five peptides. The... more
The phylogenetic relationships of the two extant lobe-finned fish, coelacanth and lungfish, to tetrapods remain unresolved. In our study of serum albumin in lungfish we determined 101 residues of protein sequence from five peptides. The lungfish sequences were aligned with the albumin superfamily using the UPGMA-based pileup or the progressive ALIGN programs. The tree construction methods employed were maximum parsimony, neighbour joining and TREE. The lungfish albumin sequence was compared with that of other species, and the resulting trees indicated a close relationship of lungfish to tetrapods, in line with the bulk of current phylogenetic evidence.
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Research Interests:
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Research Interests: Engineering, Chemistry, Marine Ecology, Biology, Biological Control, and 15 moreMedicine, Gene expression, Multidisciplinary, Bivalvia, Animals, Laboratory experiment, Enzyme, Natural Environment, Ecosystem, Bivalve, Calcium Carbonate, Heat Shock Protein, Marine ecosystem, Latitude, and Field Measurement
ABSTRACT In New Zealand chinook salmon Oncorhynchus tshawytscha, sexual genotype did not correspond to phenotype in 1·6% of male fish, contrasting starkly with North American chinook populations, where inconsistencies between female... more
ABSTRACT In New Zealand chinook salmon Oncorhynchus tshawytscha, sexual genotype did not correspond to phenotype in 1·6% of male fish, contrasting starkly with North American chinook populations, where inconsistencies between female genotype and phenotype have been observed at frequencies up to 84%.
Research Interests: Zoology, Biology, Ecology, Fish Biology, Chinook, and 4 moreNew Zealand, Fisheries Sciences, Genotype, and Macho
Research Interests:
Research Interests: Biology, Medicine, Biological Sciences, Albumin, Animals, and 15 moreNew Zealand, Physical sciences, Long Chain Fatty Acid, High Density Lipoprotein, Gel electrophoresis, Bovine Serum Albumin, Sodium Dodecyl Sulphate, Polyacrylamide Gel Electrophoresis, Protein Binding, Two dimensional Gel Electrophoresis, Anguilla, Cellulose Acetate, Palmitic Acid, Lipoprotein a, and Serum albumin
Research Interests: Biology, Membrane Proteins, Medicine, Brown trout, Albumin, and 15 moreAnimals, Atlantic Salmon, Fishery, Nickel, Ion Exchange Chromatography, Amino Acid Profile, Molecular Mass, Molecular weight, Gel electrophoresis, Amino Acid Sequence, Chinook Salmon, Propeptide, Blood Proteins, Biochemistry and cell biology, and Molecular Sequence Data
Research Interests:
Research Interests:
Research Interests: Physiology, Australia, Phylogeny, Albumin, Comparative Biochemistry, and 16 moreAnimals, Fishes, Nickel, Ion Exchange Chromatography, Glycosylation, Chickens, Amino Acid Profile, Tetrapod, Gel electrophoresis, Amino Acid Sequence, Bovine Serum Albumin, Palmitic Acid, Biochemistry and cell biology, Serum albumin, Carbohydrate Sequence, and Molecular Sequence Data
Research Interests: Physiology, Mammals, Humans, Sequence alignment, Comparative Biochemistry, and 20 moreAnimals, Fishes, Nickel, Lipoprotein(a), apolipoprotein A-I, Protein Sequence Analysis, Fatty Acid, High Density Lipoprotein, Gel electrophoresis, Two-Dimensional Gel Electrophoresis, Amino Acid Sequence, Bovine Serum Albumin, Protein Binding, Two dimensional Gel Electrophoresis, Cellulose Acetate, Palmitic Acid, Lipoprotein a, Biochemistry and cell biology, Serum albumin, and Molecular Sequence Data
There are conflicting reports over whether albumin is present in the Chondrichthyes. In addition, it is unknown whether given their low levels of extrahepatic β-oxidation, there is actually a need for a plasma FFA transport system. We... more
There are conflicting reports over whether albumin is present in the Chondrichthyes. In addition, it is unknown whether given their low levels of extrahepatic β-oxidation, there is actually a need for a plasma FFA transport system. We examined the plasma of several Chondrichthyan species, including representatives of both the Holocephali and the Neoselachii using a combination of agarose gel electrophoresis, [14C]palmitic acid binding, SDS-PAGE, and Oil Red O staining following cellulose acetate electrophoresis. None of the eight species examined had a major acidic palmitate-binding protein with the characteristics of albumin and they all lacked proteins with the mass of albumin, 66 kDa. Palmitate was bound to specific plasma proteins in all elasmobranch species examined and these palmitate-binding proteins resembled lipoproteins based on their diffuse and variable mobilities and Oil Red O staining. These lipoproteins are potentially LDL and VLDL based on their migration positions. Albumin appears to be absent from Chondrichthyan plasma and lipoproteins bind LCFA in its absence.