Md.Akhtar -E-Ekram

<p>Several studies have shown that ancient plant-derived DNA can be extracted and sequenced from lake sediments and complement the analysis of fossil pollen in reconstructing past vegetation responses to climate variability and anthropogenic perturbations. The majority of such studies have been performed on Holocene lakes located in cooler higher latitude regions whereas similar studies from tropical lakes are limited. Here, we report a ~1 Ma record of vegetation changes in tropical Lake Towuti (Sulawesi, Indonesia) through parallel pollen and sedimentary ancient DNA (sed aDNA) analysis. Lake Towuti is located in a vegetation biodiversity hotspot and in the centre of the Indo Pacific Warm Pool (IPWP), which comprises the world’s warmest oceanic waters and influences globally important climate systems. In the context of global change, the surface area of the IPWP is rapidly expanding. Lake Towuti is of particular interest since it provides a unique opportunity to obtain a long-term record of IPWP-controlled climate-ecosystem interactions and ecosystem resilience. Stratigraphic analysis of fossil pollen vs. sequencing of preserved chloroplast DNA (cpDNA) signatures (i.e., trnL-P6) both revealed that Lake Towuti experienced significant vegetation changes during the transition from a landscape initially characterized by active river channels, shallow lakes and swamps into a permanent lake ~1 Ma ago. Both proxies marked a predominance of trees or shrubs during most of Lake Towuti’s history, but the trnL-P6 barcoding approach revealed a much higher relative abundance of remote montane conifers, which likely have produced large amounts of chloroplast-rich airborne pollen that were subsequently buried in the sedimentary record. The pollen record showed a higher relative abundance of evergreen tropical forest vegetation, whereas the trnL-P6 record revealed a higher relative abundance of predominantly wetland herbs that must have entered the lake from the local catchment in the form of chloroplast-rich litter. Furthermore, the sedimentary record was rich in presumably wind-derived chloroplast-lacking fern spores, while fern trnL-P6 was only sporadically detected. Only through trnL-P6 barcoding, fern-derived biomass in the sedimentary record could be identified as Schizaeaceae, which is a primitive tropical grass-like fern family often associated with swampy or moist soils. Unlike pollen, trnL-P6 could identify grasses at clade and subfamily levels and confirmed that the majority of grasses in the area represented wet climate C3 grasses or those that can switch between C3 and C4 carbon fixation pathways, whereas grasses that can only perform C4 carbon fixation, indicative of dry climate conditions, were not detected. At least for sediments deposited prior to the Last Glacial Maximum, neither pollen nor trnL-P6 revealed significant vegetation changes between alternating layers of lacustrine green and red sideritic clays thought to have been deposited during orbitally controlled wetter vs. drier periods. These preliminary results suggest that vegetation in this tropical biodiversity hotspot may be relatively resilient to long-term variations in IPWP hydrology.</p>

Excessive use of pesticides in agricultural fields is a matter of great concern for living beings as well as the environment across the world, in particular, the third world countries. Therefore, there is an urgent need to find out an effective way to degrade these hazardous chemicals from the soil in an environment‐friendly way. In the current project, a bacterial species were isolated through enrichment culture from carbofuran‐supplemented rice‐field soil and identified as a carbofuran degrader. The rate of carbofuran degradation by this bacterial species was evaluated using reverse‐phase high‐performance liquid chromatography (RP‐HPLC), which confirmed the ability to utilize as a carbon source up to 4 µg/ml of 99% technical grade carbofuran. The morphological, physiological, biochemical characteristics and phylogenetic analysis of the 16S rRNA sequence showed that this strain belongs to the genus of Enterobacter sp. (sequence accession number LC368285 in DDBJ), and the optimum growth condition for the isolated strain was 37°C at pH 7.0. Moreover, an antibiotic sensitivity test showed that it was susceptible to azithromycin, penicillin, ceftazidime, ciprofloxacin, and gentamycin, and the minimal inhibitory concentration value of gentamycin was 400 μg/ml against the bacteria. It shows beyond doubt from the RP‐HPLC quantification that the isolated bacterium has the ability to detoxify carbofuran (99% pure). Finally, the obtained results imply that the isolated strain of Enterobacter can be used as a potential and effective carbofuran degrader for bioremediation of contaminated sites through bioaugmentation.

Context: Isolated plasmid DNA from salt tolerant bacteria can play an important role in special environmental conditions. Objectives: The main objective of this study was to make a standard protocol for the isolation of large plasmid DNA from a highly salt tolerant ...

A Single bacterial colony was isolated by plating the aseptically separated crushed-cuticle sample of Penaeus monodon on an agar solidified LB medium, supplemented with 10gm/100ml salt concentration. The bacterium was found to be gram positive, non-motile, aerobic and lactose non-fermenting through morphological and biochemical tests. The optimum culture condition of the isolate was at pH 7.5 with temperature of 30 C. The bacteria showed the ability of utilizing arabinose, sucrose, maltose, fructose as carbon sources and viable cell count was 489×107CFU/mL. Isolated bacterium showed citrate metabolism ability, the ability of breaking down the amino acid tryptophan into indole and no catalase activity. The bacterium was resistant to amoxicillin, penicillin and vancomycin and the MIC value against penicillin was 100mg/mL. The bacterial colony was also specially characterized for CMCase production activity. The molecular weight of isolated bacterial genomic DNA was above 10 kb with not...