Genetic diversity is the best way to improve available genetic resources for breeding programs in... more Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymorphic DNA (RAPD) markers. Average polymorphism information content (PIC) for ISSR, DAMD and RAPD markers was 0.40, 0.41 and 0.40, respectively. Cluster analysis for ISSR, DAMD and RAPD divided the cultivars into three distinct clusters. The comparative analysis of the three markers (ISSR, DAMD and RAPD) showed that DAMD had superiority over RAPD and ISSR in characterization of genetic diversity in Gerbera. To our knowledge, this is the first report of a comparison of performance among DAMD, ISSR and RAPD techniques on a set of gerbera genotypes. Overall, our results showed that DAMD markers well represented different genotypes of gerbera diversity.
Studia Universitatis Babeş-Bolyai. Biologia, Jun 30, 2024
The COVID-19 is considered as a type of severe acute respiratory syndrome (SARS-CoV-2). The curre... more The COVID-19 is considered as a type of severe acute respiratory syndrome (SARS-CoV-2). The current pandemic causes a vital destruction in international social and economic systems. Current available vaccines involve entire viruses; however, peptide-based vaccines could be also beneficial. In the present study, a computationally candidate vaccine was designed against SARS-CoV-2. Surface glycoproteins (E, M, and S proteins) and N protein amino acid sequences were analyzed to predict high score of the B and T cell epitopes as antigenic proteins of the virus. High score epitopes, and the B subunit of Vibrio cholerae toxin, as an adjuvant put together by appropriate linkers to construct a multi-epitope candidate vaccine. Bioinformatics tools were used to predict the secondary, tertiary structure and physicochemical properties, such as aliphatic index, theoretical pH, molecular weight, and estimated half-life of the multi-epitope candidate vaccine. The interaction of candidate vaccine with TLR2 and TLR4 was computationally evaluated by molecular docking. Finally, the codon optimization and the secondary structure of mRNA were calculated, and in silico cloning was performed into plant expression vector by SnapGENE. This designed candidate vaccine along with the computational results requires laboratory evaluations to be confirmed as a candidate vaccine against SARS-COV-2 infection.
Malaria parasites can affect vector-related behaviours, increasing transmission success. Using An... more Malaria parasites can affect vector-related behaviours, increasing transmission success. Using Anopheles gambiae and Plasmodium falciparum, we consider the effect of interaction between infection stage and vector age on diel locomotion in response to human odour and the expression of antennal chemosensory genes. We demonstrate age-dependent behavioural diel compartmentalisation by uninfected females. Infection disrupts overall and diel activity patterns compared with age-matched controls. Mosquitoes carrying transmissible sporozoites are more active, shifting activity periods to coincide with human host availability, in response to human odour. Older, uninfected females reduce activity during their peak host-seeking period in response to human odour. Age- and infection stage-specific changes in odour-mediated locomotion coincide with altered transcript abundance of select chemosensory genes providing a possible molecular mechanism regulating the behaviour. Our results support the hy...
Recent data show that parasites manipulate the physiology of mosquitoes and human hosts to increa... more Recent data show that parasites manipulate the physiology of mosquitoes and human hosts to increase the probability of transmission. Here, we investigate phagostimulant activity of Plasmodium-metabolite, (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), in the primary vectors of multiple human diseases, Anopheles coluzzii, An. arabiensis, An. gambiae s.s., Aedes aegypti, and Culex pipiens/Culex torrentium complex species. The addition of 10 µM HMBPP to blood meals significantly increased feeding in all the species investigated. Moreover, HMBPP also exhibited a phagostimulant property in plant-based-artificial-feeding-solution made of beetroot juice adjusted to neutral pH similar to that of blood. The addition of AlbuMAXTM as a lipid/protein source significantly improved the feeding rate of An. gambiae s.l. females providing optimised plant-based-artificial-feeding-solution for delivery toxins to control vector populations. Among natural and synthetic toxins tested, only fipro...
Oat is an important cereal crops and it has forth place among them. Nevertheless, growing oat is ... more Oat is an important cereal crops and it has forth place among them. Nevertheless, growing oat is not very common in Iran. In order to investigate genetic diversity and describe the relationships between seed yield and its components, 16 cultivars of oat were studied using, molecular traits. Investigating genetic and molecular diversity was performed by using 10 primers of SSR markers. Collected data were analyzed. In analysis into main components special values resulted from 1-5 component were bigger than one and in total they justified 76% of all variables variances. Molecular diversity of cultivars was measured using parameters of polymorphic information, the number of effective allele, Shannon index and the average of 48/81 was achieved among them 20/62 bands were polymorphic. The average of bands number varies from 1/81 to 2/31 for each marker. The maximum and minimum of polymorphic band belong to the marker Bmac0040 with 2/31 and marker Gms001 with 1/81 respectively. Also the average of the maximum bands (5/9) related to Possum cultivar and the minimum band (3/0) belonged to CN12497 cultivar. According to molecular data, average comparison of oat genotypes have been shown that maximum bands average related to Possum genotype. Swan genotype created highest percentage of polymorphism.Also HVHVA1 was most effective primer and created most diversity based on the percentage of polymorphism,band number and value of polymorphism.
Genetic diversity is the best way to improve available genetic resources for breeding programs in... more Genetic diversity is the best way to improve available genetic resources for breeding programs in gerbera. In present study, genetic diversity of 22 gerbera cultivars was investigated using inter-simple sequence repeat (ISSR), directly amplified minisatellite DNA (DAMD), and Random Amplified Polymorphic DNA (RAPD) markers. Average polymorphism information content (PIC) for ISSR, DAMD and RAPD markers was 0.40, 0.41 and 0.40, respectively. Cluster analysis for ISSR, DAMD and RAPD divided the cultivars into three distinct clusters. The comparative analysis of the three markers (ISSR, DAMD and RAPD) showed that DAMD had superiority over RAPD and ISSR in characterization of genetic diversity in Gerbera. To our knowledge, this is the first report of a comparison of performance among DAMD, ISSR and RAPD techniques on a set of gerbera genotypes. Overall, our results showed that DAMD markers well represented different genotypes of gerbera diversity.
Studia Universitatis Babeş-Bolyai. Biologia, Jun 30, 2024
The COVID-19 is considered as a type of severe acute respiratory syndrome (SARS-CoV-2). The curre... more The COVID-19 is considered as a type of severe acute respiratory syndrome (SARS-CoV-2). The current pandemic causes a vital destruction in international social and economic systems. Current available vaccines involve entire viruses; however, peptide-based vaccines could be also beneficial. In the present study, a computationally candidate vaccine was designed against SARS-CoV-2. Surface glycoproteins (E, M, and S proteins) and N protein amino acid sequences were analyzed to predict high score of the B and T cell epitopes as antigenic proteins of the virus. High score epitopes, and the B subunit of Vibrio cholerae toxin, as an adjuvant put together by appropriate linkers to construct a multi-epitope candidate vaccine. Bioinformatics tools were used to predict the secondary, tertiary structure and physicochemical properties, such as aliphatic index, theoretical pH, molecular weight, and estimated half-life of the multi-epitope candidate vaccine. The interaction of candidate vaccine with TLR2 and TLR4 was computationally evaluated by molecular docking. Finally, the codon optimization and the secondary structure of mRNA were calculated, and in silico cloning was performed into plant expression vector by SnapGENE. This designed candidate vaccine along with the computational results requires laboratory evaluations to be confirmed as a candidate vaccine against SARS-COV-2 infection.
Malaria parasites can affect vector-related behaviours, increasing transmission success. Using An... more Malaria parasites can affect vector-related behaviours, increasing transmission success. Using Anopheles gambiae and Plasmodium falciparum, we consider the effect of interaction between infection stage and vector age on diel locomotion in response to human odour and the expression of antennal chemosensory genes. We demonstrate age-dependent behavioural diel compartmentalisation by uninfected females. Infection disrupts overall and diel activity patterns compared with age-matched controls. Mosquitoes carrying transmissible sporozoites are more active, shifting activity periods to coincide with human host availability, in response to human odour. Older, uninfected females reduce activity during their peak host-seeking period in response to human odour. Age- and infection stage-specific changes in odour-mediated locomotion coincide with altered transcript abundance of select chemosensory genes providing a possible molecular mechanism regulating the behaviour. Our results support the hy...
Recent data show that parasites manipulate the physiology of mosquitoes and human hosts to increa... more Recent data show that parasites manipulate the physiology of mosquitoes and human hosts to increase the probability of transmission. Here, we investigate phagostimulant activity of Plasmodium-metabolite, (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), in the primary vectors of multiple human diseases, Anopheles coluzzii, An. arabiensis, An. gambiae s.s., Aedes aegypti, and Culex pipiens/Culex torrentium complex species. The addition of 10 µM HMBPP to blood meals significantly increased feeding in all the species investigated. Moreover, HMBPP also exhibited a phagostimulant property in plant-based-artificial-feeding-solution made of beetroot juice adjusted to neutral pH similar to that of blood. The addition of AlbuMAXTM as a lipid/protein source significantly improved the feeding rate of An. gambiae s.l. females providing optimised plant-based-artificial-feeding-solution for delivery toxins to control vector populations. Among natural and synthetic toxins tested, only fipro...
Oat is an important cereal crops and it has forth place among them. Nevertheless, growing oat is ... more Oat is an important cereal crops and it has forth place among them. Nevertheless, growing oat is not very common in Iran. In order to investigate genetic diversity and describe the relationships between seed yield and its components, 16 cultivars of oat were studied using, molecular traits. Investigating genetic and molecular diversity was performed by using 10 primers of SSR markers. Collected data were analyzed. In analysis into main components special values resulted from 1-5 component were bigger than one and in total they justified 76% of all variables variances. Molecular diversity of cultivars was measured using parameters of polymorphic information, the number of effective allele, Shannon index and the average of 48/81 was achieved among them 20/62 bands were polymorphic. The average of bands number varies from 1/81 to 2/31 for each marker. The maximum and minimum of polymorphic band belong to the marker Bmac0040 with 2/31 and marker Gms001 with 1/81 respectively. Also the average of the maximum bands (5/9) related to Possum cultivar and the minimum band (3/0) belonged to CN12497 cultivar. According to molecular data, average comparison of oat genotypes have been shown that maximum bands average related to Possum genotype. Swan genotype created highest percentage of polymorphism.Also HVHVA1 was most effective primer and created most diversity based on the percentage of polymorphism,band number and value of polymorphism.
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Papers by Melika Hajkazemian