Pierre Leprince
Université de Liège, GIGA Neurosciences, Faculty Member
- PI and teacher in a Neuroscience and Biomedical contextedit
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In this study, a quantitative comparative proteomics approach has been used to analyze the Dictyostelium discoideum mitochondrial proteome variations during vegetative growth, starvation and the early stages of development. Application of... more
In this study, a quantitative comparative proteomics approach has been used to analyze the Dictyostelium discoideum mitochondrial proteome variations during vegetative growth, starvation and the early stages of development. Application of 2-D DIGE technology allowed the detection of around 2000 protein spots on each 2-D gel with 180 proteins exhibiting significant changes in their expression level. In total, 96 proteins (51 unique and 45 redundant) were unambiguously identified. We show that the D. discoideum mitochondrial proteome adaptations mainly affect energy metabolism enzymes (the Krebs cycle, anaplerotic pathways, the oxidative phosphorylation system and energy dissipation), proteins involved in developmental and signaling processes as well as in protein biosynthesis and fate. The most striking observations were the opposite regulation of expression of citrate synthase and aconitase and the very large variation in the expression of the alternative oxidase that highlighted the importance of citrate and alternative oxidase in the physiology of the development of D. discoideum. Mitochondrial energy states measured in vivo with MitoTracker Orange CM Ros showed an increase in mitochondrial membrane polarization during D. discoideum starvation and starvation-induced development.
Research Interests: Microbiology, Biology, Proteomics, Comparative Proteomics, Cell Biology, and 15 moreOxidative Stress, Mitochondria, Medicine, Signal Transduction, Proteostasis, Biological Sciences, Dictyostelium, Phosphorylation, Oxidative phosphorylation, Gel electrophoresis, Proteome, Alternative Oxidase, Mitochondrion, Medical and Health Sciences, and Dictyostelium discoideum
Research Interests: Biology, Proteomics, Cell Biology, Mitochondria, Medicine, and 15 moreIon Channels, Biological Sciences, Animals, Male, ATP synthase, Body temperature regulation, CHEMICAL SCIENCES, Brown Adipose Tissue, Rats, Acclimatization, Adipocytes, Proteome, Cold Acclimation, Mitochondrial Proteins, and Cold Temperature
<b>Copyright information:</b>Taken from "Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4"BMC Neuroscience 2004;5():33-33.Published... more
<b>Copyright information:</b>Taken from "Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4"BMC Neuroscience 2004;5():33-33.Published online 15 Sep 2004PMCID:PMC520747.Copyright © 2004 Wislet-Gendebien et al; licensee BioMed Central Ltd. We observed that 14.4 ± 7.1%, 7.1± 4.1% and 3.4 ± 1.6% of cells were respectively GFAP-, Tuj1- and O4-positive (these data were obtained by absolute counts on 1576 cells). (B-C) The proliferative capacity of the differentiating neurosphere-derived cells placed in npMSC conditioned medium, nnMSC conditioned medium and in control medium (DEM/F12 + B27) was compared. BrdU incorporation was performed after 48 hours and 4 days in differentiating conditions. Double labelling GFAP-, Tuj1- and O4 with BrdU were performed. The BrdU incorporation by differentiating neurosphere-derived cells placed in the various conditions did not shown significant differences (Statistical test ANOVA, P &gt; 0.05). (D-E) The cell death quantified by propidium iodide incorporation and counting was analysed after 48 hours and 4 days in differentiating conditions and in GFAP-, Tuj1- and O4-positive cells. After 48 hours, a significant decrease of the number of GFAP-positive cells which have incorporated the propidium iodide is observed (***Student T test, p &lt; 0.0001). However, no significant increase of cell death was observed in O4- and Tuj1-positive cell population (Student T test, p &gt; 0.05). After 4 days of differentiation, no difference in cell death is observed whatever the cell type or the culture condition.
<b>Copyright information:</b>Taken from "Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4"BMC Neuroscience 2004;5():33-33.Published... more
<b>Copyright information:</b>Taken from "Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4"BMC Neuroscience 2004;5():33-33.Published online 15 Sep 2004PMCID:PMC520747.Copyright © 2004 Wislet-Gendebien et al; licensee BioMed Central Ltd. After 5 days, the cells were fixed and immunostained with antibodies raised against GFAP (A, D, red), Tuj1 (B, E, red) and O4 (C, F, red). Nuclei were counterstained with EtD1 (blue). Co-cultures with npMSCs are illustrated in A (n = 12, ***Student T-test, P &lt; 0.001), B (n = 12, ***Student T test, P &lt; 0.0001) and C (n = 8, ***Student T test, P &lt; 0.0001). Co-cultures with nnMSCs are illustrated in D (n = 8, Student T test, P &gt; 0.05), E (n = 8, ***Student T test, P &lt; 0.001) and F (n = 8, Student T test, P &gt; 0.05). Stained cells counting results are shown in G and include the control condition of neurosphere-derived cells without co-culture. Scale bars = 20 μm in A-F.
<b>Copyright information:</b>Taken from "Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4"BMC Neuroscience 2004;5():33-33.Published... more
<b>Copyright information:</b>Taken from "Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4"BMC Neuroscience 2004;5():33-33.Published online 15 Sep 2004PMCID:PMC520747.Copyright © 2004 Wislet-Gendebien et al; licensee BioMed Central Ltd. The results of quantitative RT-PCR are expressed as percent of gene expression in npMSCs compared to nnMSCs (arbitrarily as 100%) after normalisation with the GAPDH house-keeping gene expression. (B) Western blotting analysis of conditioned media by npMSCs, nnMSCs and NSC. The mature and biologically-active form of BMP4 (27 kDa) is only observed in medium conditioned by npMSCs and the 57 kDa immature and non biologically-active form can be seen in nnMSC and in neural stem cells conditioned medium. This high MW form is not present in npMSC conditioned medium, indicating a complete cleavage and activation of BMP4. (C) Effect of anti-BMP4 antibodies in differentiating NSCs cultivated in DEM/F12 or npMSCs conditioned medium. Anti-BMP4 antibodies inhibit the npMSCs effect on astroglial and oligodendroglial cell numbers but not the effect on neurons number (47.13 ± 1.53% of GFAP-positive cells, 9.98 ± 0.92% of Tuj1-positive cells and 5.67 ± 0.45% of O4-positive cells with anti-BMP4 compared to respectively 77.5 ± 2.5%, 4.30 ± 0.95% and 1.47± 0.60% in npMSC conditioned-medium). Tuj1-positive cells remains significantly lower (***student T test, p &lt; 0.001, n = 5).
band volume ratio from western blot analysis was -1.28 (n = 3). Average ratio from 2D-DIGE analysis of the spot containing alpha-SNAP was -1.37 (n = 9); average band volume ratio from western blot analysis was -1.15 (n = 3). For all... more
band volume ratio from western blot analysis was -1.28 (n = 3). Average ratio from 2D-DIGE analysis of the spot containing alpha-SNAP was -1.37 (n = 9); average band volume ratio from western blot analysis was -1.15 (n = 3). For all western blots, the variations in sample loading revealed by the actin signal is taken into account for the calculation of the average band volume ratio.<b>Copyright information:</b>Taken from "Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation"http://www.proteomesci.com/content/6/1/14Proteome Science 2008;6():14-14.Published online 22 May 2008PMCID:PMC2453108.
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Radial glial cells play a major guidance role for migrating neurons during central nervous system (CNS) histogenesis but also play many other crucial roles in early brain development. Being among the earliest cells to differentiate in the... more
Radial glial cells play a major guidance role for migrating neurons during central nervous system (CNS) histogenesis but also play many other crucial roles in early brain development. Being among the earliest cells to differentiate in the early CNS, they provide support for neuronal migration during embryonic brain development; provide instructive and neurotrophic signals required for the survival, proliferation, and differentiation of neurons; and may be multipotential progenitor cells that give rise to various cell types, including neurons. Radial glial cells constitute a major cell type of the developing brain in numerous nonmammalian and mammalian vertebrates, increasing in complexity in parallel with the organization of the nervous tissue they help to build. In mammalian species, these cells transdifferentiate into astrocytes when neuronal migration is completed, whereas, in nonmammalian species, they persist into adulthood as a radial component of astroglia. Thus, our percepti...
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ABSTRACT Normal brain functions are to the highest degree dependent on the cytoarchitecture and the intercellular relationships that govern both the nervous system metabolism (for instance, the integrity of the blood-brain barrier) and... more
ABSTRACT Normal brain functions are to the highest degree dependent on the cytoarchitecture and the intercellular relationships that govern both the nervous system metabolism (for instance, the integrity of the blood-brain barrier) and specific functions (synaptic transmission, glioneuronal relationships). Thus, the study of the biology of the nerve cells should be approached using experimental designs that preserve the histological structure in which these cells take part in vivo.
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Research Interests: Endocrinology, Neuroscience, Psychology, Kinetics, Biology, and 15 moreImmunohistochemistry, Medicine, In Situ Hybridization, Internal Medicine, Cerebral Cortex, Animals, Male, Neurons, Astrocytes, Fibroblast Growth Factor, Phenotype, Neurotransmitter, Neurosciences, Ciliary Neurotrophic Factor, and Calcitonin Gene Related Peptide
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Regressive events are common in developmental biology and the nervous system does not make exception. Indeed, neuronal death is a characteristic feature of nervous system development in vertebrates, although its quantitative importance... more
Regressive events are common in developmental biology and the nervous system does not make exception. Indeed, neuronal death is a characteristic feature of nervous system development in vertebrates, although its quantitative importance may vary according to species and anatomical location. It is thought that the adaptative significance of the so-called developmental neuronal death is to match the size of the pool of innervating neurons to the size of target territories. It is from studies aimed at understanding the mechanism(s) of neuronal number regulation during development that the concept of neuronotrophic influences and factors stems. A question that has been addressed more recently relates to the role of trophic influences in regular neuronal maintenance and most importantly in regeneration. Implicit in the question concerning the role of trophic factors in regular neuronal maintenance is that relating to a possible deficit of trophic factor(s) leading to the neuronal death observed in neurodegenerative diseases. Alternatively, neuronal death that occurs in those chronic disorders might result from the existence of endogenous or exogenous toxic substances and effects which are not counterbalanced by adequate (both quantitatively and qualitatively) trophic environment. Indeed, during the last decade, exogenous and endogenous toxic substances have been found which are able to induce acute or chronic neuronal death and which might be implicated in the pathophysiology of neuronal loss in various neurological diseases.
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Regressive events are common in developmental biology and the nervous system does not make exception. Indeed, neuronal death is a characteristic feature of nervous system development in vertebrates, although its quantitative importance... more
Regressive events are common in developmental biology and the nervous system does not make exception. Indeed, neuronal death is a characteristic feature of nervous system development in vertebrates, although its quantitative importance may vary according to species and anatomical location. It is thought that the adaptative significance of the so-called developmental neuronal death is to match the size of the pool of innervating neurons to the size of target territories. It is from studies aimed at understanding the mechanism(s) of neuronal number regulation during development that the concept of neuronotrophic influences and factors stems. A question that has been addressed more recently relates to the role of trophic influences in regular neuronal maintenance and most importantly in regeneration. Implicit in the question concerning the role of trophic factors in regular neuronal maintenance is that relating to a possible deficit of trophic factor(s) leading to the neuronal death observed in neurodegenerative diseases. Alternatively, neuronal death that occurs in those chronic disorders might result from the existence of endogenous or exogenous toxic substances and effects which are not counterbalanced by adequate (both quantitatively and qualitatively) trophic environment. Indeed, during the last decade, exogenous and endogenous toxic substances have been found which are able to induce acute or chronic neuronal death and which might be implicated in the pathophysiology of neuronal loss in various neurological diseases.
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audience: researcher, professional, student, popularizationTrop fréquemment, l’expérimentation animale est présentée comme une pratique archaïque. Elle a bien changé. Et 100 % des patients traités le sont grâce aux concepts et techniques... more
audience: researcher, professional, student, popularizationTrop fréquemment, l’expérimentation animale est présentée comme une pratique archaïque. Elle a bien changé. Et 100 % des patients traités le sont grâce aux concepts et techniques développés grâce à elle
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Contact : zlacrosse@ulg.ac.be Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness”... more
Contact : zlacrosse@ulg.ac.be Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness” (DOMS). DOMS include muscle pain that appears 24 to 72 hours after exercise, but also stiffness, edema and muscle proteins release in plasma. The only systematic intervention that brings a muscle protection against DOMS is to realize submaximal eccentric contractions with a progressively increased intensity. The mechanism of this protection, called the “Repeated Bout Effect” (RBE), is not understood. However, it is likely explained by cellular, mechanical and neural theories [Scand.J.Med.&Sci.Sports, 13, 88, 2003].