Department of Plant Breeding and Biotechnology

The aim of this study was to investigate the correlation between biochemical and molecular markers in Eucalyptus microtheca F. Muell. under in vitro culture. For this mean, twig-derived explants obtained from Eucalyptus microtheca 1-year-old seedling were cultured on modified MS medium, supplemented with different concentrations of NAA, Kin and TDZ. POD (peroxidase) alleles patterns were studied among regenerated plantlets to investigate the effect of TDZ concentration on POD activity. A dimer locus, a tetramer locus and two epigenetic bands were observed. Genome variation among somaclonal plantlets were investigated using microsatellite markers. SSR (Simple Sequence Repeat) markers revealed polymorphism among the studied population. Nonparametric statistical analysis showed significant effect of simple sequence repeats loci on peroxidase alleles. Correlation of two similarity matrix POD and SSRs loci was 0.18 using Mental test. Results showed less stability of dimer locus in different concentrations of TDZ compared to tetramer locus. Tetramer alleles showed more correlation to SSRs than that of dimmer ones.

Background: Hazelnut (Corylus avellana) is a species belonging to the genus Corylus. Its various segments contain different metabolites with very valuable medicinal, anti-microbial and anti-cancer properties. Objective: The present study was done to investigation the optimal conditions for hazelnut plants regenerated from cotyledon as explants and invitrotaxol production. Methods: Cotyledon segments were cultured on MS medium containing 2,4-D concentrations of 0, 0.5 and 1 mgl-1 alone or in combination with concentrations of 0, 0.5 and mgl-1 BA for callus induction. For regeneration, Calli were sub-cultured to the same medium and or transferred to the MS medium containing BA at concentrations of 0, 0.25, 0.5, 1, 2 and 3 mgl-1 Callus was formed in all hormonal treatments. The ability of taxol production in calli was evaluated by adding methyl jasmonat in 0, 10, 40, 70, 100, 130 and 160 μM concentrations. Results: In hormonal treatment including 0.5 mgl-1 2.4-D regeneration was observ...

Common hazel (Corylus avallena) is extensively cultivated in North America, Northwest Asia and Europe; consumed both in raw and processed forms, as well as in pharmaceutical industries. The latter makes it a valuable species for in vitro propagation. Several studies have reported embryogenesis from different plant organs. This study is focused on producing embryos from petiole and leaf-derived callus cultures.Leaf (1×1cm 2 segments) and petiole (1cm) cuttings were surface sterilized and subcultured in MS medium containing gradient combinations of 2,4-D+BAP, cultures refreshed every four weeks. Each growth regulator combination was replicated three times, with five cutting in each container. The calli were then moved to MS media (both solid and liquid forms) supplemented with gradient levels of 2,4-D+BAP, IBA+BAP, and IBA+Kin for embryogenesis stage; maintained under standard growth chamber conditions. The highest frequency of pre-embryo structure was obtained under 0.1 mg.l -1 2,4-D...

Background & Objectives: Most of the infections caused by Staphylococcus aureus resistantstrains are originated from hospitals, and its prevalence is increasing worldwide. Therefore, many efforts have been made to find new compounds as a substitute for antibiotics. This study investigates the antibacterial effect of alcoholic extract and essential oils of Myrtus communis L against resistant Staphylococcus aureus strain.

In order to investigation of genetic variation among lentil lines and genetic relationship these to Cistan local cultivar, DNA of 20 lentil lines and Cistan local cultivar were analysed using random amplified polymorphic DNA (RAPD) and intron-exon splice junctions (ISJ) markers. PIC, MI, percentage of polymorphic bands (P), Nei , s gene diversity (H), Shannon , s information index (I) were calculated for both random, semi random and combined of random and semi random primers. For both matrices, genetic similarity (GS RAPD and GS ISJ ) were calculated according to the Jaccard similarity coefficient. Correlation between two similarity matrices obtained with two markers types was estimated. The similarity matrix per marker was subjected to cluster analysis by the unweighted pair-group method (UPGMA). The C PI of RAPD was as C PI as ISJ, but MI index of ISJ (average 6.095) was much higher than RAPD (average 4.49). Mean of genetic similarity of RAPD marker was higher than ISJ marker, and...

In order to correlation and board sense inheritance was performed experimentally under randomized completely blocked design (RCBD) with two replication in the institute of agriculture and natural resources. Results exhibited that height of flag leaf had positive and significant correlation to height of peduncle, height of stem, height of spike, day to flowering and day to seed beginning. Height of peduncle also had positive and significant correlation to height of stem, height of spike, day to flowering and day to seed beginning under normal conditions. Height of flag leaf to height of out stem from flag leaf, width of flag leaf to width of spike, height of peduncle to height of spike and day to seed beginning had positive and significant correlation under drought stress conditions. Traits such as: width of flag leaf, length of flag leaf, length of stem, day to flowering, number seed per spike and weight of thousand seed had the most heritability and height of peduncle, height of ou...