Research Interests:
Myelin basic protein (MBP) is a multifunctional protein involved in maintaining the stability and integrity of the myelin sheath by a variety of interactions with membranes and with cytoskeletal and other proteins. A central segment of... more
Myelin basic protein (MBP) is a multifunctional protein involved in maintaining the stability and integrity of the myelin sheath by a variety of interactions with membranes and with cytoskeletal and other proteins. A central segment of MBP is highly conserved in mammals and consists of a membrane surface-associated amphipathic R-helix, immediately followed by a proline-rich segment that we hypothesize is
Research Interests: Biochemistry, Molecular Recognition, Computer Simulation, Circular Dichroism, Lipid Membranes, and 11 moreMolecular docking, Proline, Mitogen Activated Protein Kinase, Protein Secondary Structure Prediction, Calmodulin, Molecular Conformation, Amino Acid Sequence, Protein Binding, Ligands, Binding Site, and Biochemistry and cell biology
Research Interests:
We have demonstrated that sonic hedgehog (Shh), vital for oligodendrocyte development, is present in both gray and white matter of normal human brain. Both the 45 kDa precursor protein and the 20 kDa N-terminal sonic hedgehog signaling... more
We have demonstrated that sonic hedgehog (Shh), vital for oligodendrocyte development, is present in both gray and white matter of normal human brain. Both the 45 kDa precursor protein and the 20 kDa N-terminal sonic hedgehog signaling portion (ShhN) were demonstrated by immunoblot and a partial purification has been achieved. In gray matter from brains of multiple sclerosis (MS) victims, the total amount of Shh was less than normals and the signaling 20 kDa protein was greatly reduced. In white matter homogenates, prepared from MS victims, only the 45 kDa precursor protein was found. None of the 20 kDa signaling protein was detected, suggesting that the 45 kDa signaling protein was not cleaved in the autocatalytic reaction carried out by the C-terminal portion. The 45 kDa protein and a small amount of the 20 kDa ShhN was detected in isolated MS myelin by Western blot, demonstrating some cleavage was possible. The cleavage of the 45 kDa protein was demonstrated in normal myelin in vitro, but not in myelin prepared from MS brain.
Research Interests:
Research Interests:
Research Interests:
Research Interests: Biochemistry, Multiple sclerosis, Lipid Membranes, Mice, Animals, and 17 moreActin Cytoskeleton, Calmodulin, Arginine, Electrostatic force, Protein isoforms, Lipid bilayers, Amino Acid Sequence, Citrulline, Demyelinating disease, Recombinant Proteins, Protein Binding, Lipid Bilayer, Actin Filaments, Site-directed Mutagenesis, Static Electricity, Dissociation Constant, and Biochemistry and cell biology
Research Interests:
ABSTRACT: The basic protein of myelin may be involved in association of the cytoplasmic surfaces of myelin by a bridging mechanism whereby the N-terminal half of the protein binds to one surface and the C-terminal half binds to the... more
ABSTRACT: The basic protein of myelin may be involved in association of the cytoplasmic surfaces of myelin by a bridging mechanism whereby the N-terminal half of the protein binds to one surface and the C-terminal half binds to the opposing surface. The feasibility of this mechanism ...