V. Rutten
Utrecht University, Infectious Diseases and Immunology, Faculty Member
Research Interests:
Research Interests: Immune response and Plant
Research Interests:
Research Interests:
In all species studied so far, gammadelta T cells are abundantly present in epithelia. The functions of these cells are largely unknown. Using a lymph duct cannulation method, which is only possible in large animals such as cattle, we... more
In all species studied so far, gammadelta T cells are abundantly present in epithelia. The functions of these cells are largely unknown. Using a lymph duct cannulation method, which is only possible in large animals such as cattle, we show that large numbers of gammadelta T cells, but not alphabeta T cells, are constitutively present in pseudoafferent lymph draining bovine skin. The gammadelta T cells, which are present in pseudoafferent lymph, use Vgamma segments that are characteristic for bovine dermal gammadelta T cells, suggesting that these cells migrated from the skin. Further supporting the origin of these cells is the fact that fluorescent latex beads injected in the skin could be recovered in cells in the pseudoafferent lymph. The cannulation method is minimally invasive, and the lymph flow, which was sustained and remained essentially unaltered during 14 days, closely represents the in vivo situation. The gammadelta T cells could not be induced to produce IFN-gamma, TNF-a...
Research Interests: Immunology, Cell Migration, Cytokines, Female, Animals, and 6 moreMale, Skin, MHC class II, Cattle, T lymphocytes, and Minimally Invasive
Allergen specific IgGd antibodies were detected by the enzyme linked immunosorbent assay (ELISA) in 89% of the 62 atopic dogs studied. Antibodies were found most frequently against house dust (47%), human dander (50%), grass pollens (58%)... more
Allergen specific IgGd antibodies were detected by the enzyme linked immunosorbent assay (ELISA) in 89% of the 62 atopic dogs studied. Antibodies were found most frequently against house dust (47%), human dander (50%), grass pollens (58%) and spring tree pollens (43%). These antibodies were also found in 11 of 20 dogs with atopic symptoms but without immediate skin test reactivity to inhalant allergens. Agreement between the presence of skin reactivity and allergen specific IgGd titres ranged from one of 14 for cat dander to 22 of 34 for house dust. Among dogs with atopic symptoms but without skin test reactivity and specific IgGd titres to the respective allergens, the agreement varied between 28 of 54 for human dander and 67 of 68 for cat dander. In view of the value of the dog as an experimental model of atopic disease in man, further studies of the pathophysiological significance of IgGd antibodies are warranted. In addition, reconsideration of the diagnostic criteria for canine...
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Dogs immunized with o-DNCP-BSA or eggs of Toxocara canis developed anaphylactic antibodies other than IgE. The antibodies were heat-resistant at 56 degrees C for 2 hours, not reduced by 2-mercaptoethanol and PCA and P-K reactivity was... more
Dogs immunized with o-DNCP-BSA or eggs of Toxocara canis developed anaphylactic antibodies other than IgE. The antibodies were heat-resistant at 56 degrees C for 2 hours, not reduced by 2-mercaptoethanol and PCA and P-K reactivity was only observed at 3-4 h sensitized sites of recipient skin. High titres of antigen-specific IgGd were determined by means of an ELISA technique.
Research Interests:
Research Interests:
Strong anti glycolipid IgG responses can occur in humans and animals, but contrary to anti protein responses and anti glycoprotein responses, the exact mechanism of induction is unknown. We have previously shown that experimental... more
Strong anti glycolipid IgG responses can occur in humans and animals, but contrary to anti protein responses and anti glycoprotein responses, the exact mechanism of induction is unknown. We have previously shown that experimental immunization with the glycolipid glucose monomycolate (GMM) causes the development of specific T cell responses, but not of anti GMM antibodies. However, cattle naturally infected with Mycobacterium avium ssp. paratuberculosis produce high levels of anti GMM IgG. In the present study, we tested whether vaccination with GMM conjugated to a protein mimics natural infection in its capacity to induce the production of antibodies against GMM. Cattle were immunized (n=5 per group) with GMM conjugated to a protein, or GMM and protein non-conjugated and administered at contralateral locations, or carrier only. Although immunization with the GMM-protein conjugate vaccine and the non-conjugated vaccine induced protein specific antibody responses, GMM specific antibod...
Research Interests: Zoology, Immune response, Mycobacterium tuberculosis, Animals, P-glycoprotein, and 14 moreImmunization, Cattle, T lymphocytes, Glycolipids, Veterinary Sciences, Cell Proliferation, Mycobacterium bovis, Immunoglobulin, Paratuberculosis, Hemocyanin, Antibody Response, mycobacterium avium ssp. paratuberculosis, cattle diseases, and Mycobacterium avium subsp. paratuberculosis (MAP)
Glycolipids are presented to T cells by human group 1 CD1 proteins, but are not used as subunit vaccines yet. Experimental immunizations with pure mycobacterial glucose monomycolate (GMM) and keyhole limpet haemocyanin (KLH) in cattle, a... more
Glycolipids are presented to T cells by human group 1 CD1 proteins, but are not used as subunit vaccines yet. Experimental immunizations with pure mycobacterial glucose monomycolate (GMM) and keyhole limpet haemocyanin (KLH) in cattle, a species which, unlike mice, expresses group 1 CD1, showed that GMM was equally efficient as KLH in generating T cell responses in blood, but not in the draining lymph node. Also, KLH induced strong antibody responses whereas GMM did not. These data suggest that non-overlapping T cell populations are targeted and demonstrate the potential of glycolipids as a special class of subunit vaccine candidates.