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Biochim Biophys Acta. Author manuscript; available in PMC 2011 August 1.
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Published in final edited form as:
Biochim Biophys Acta. 2010 August ; 1800(8): 858–870. doi:10.1016/j.bbagen.2010.03.012.
The sedimentation properties of ferritins. New insights and
analysis of methods of nanoparticle preparation
Carrie A. May†, John K. Grady†,§, Thomas M. Laue‡, Maura Poli∥, Paolo Arosio∥, and N.
Dennis Chasteen†,*
†Department of Chemistry, University of New Hampshire, Durham, NH 03824-2544
‡Center
to Advance Molecular Interaction Science, University of New Hampshire, Durham, NH
03824-2544
∥Department
Materno Infantile e Tecnologie Biomediche, University of Brescia, 25125 Italy
Abstract
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Background—Ferritin exhibits complex behavior in the ultracentrifuge due to variability in iron
core size among molecules. A comprehensive study was undertaken to develop procedures for
obtaining more uniform cores and assessing their homogeneity.
Methods—Analytical ultracentrifugation was used to measure the mineral core size distributions
obtained by adding iron under high- and low-flux conditions to horse spleen (apoHoSF) and
human H-chain (apoHuHF) apoferritins.
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Results—More uniform core sizes are obtained with the homopolymer human H-chain ferritin
than with the heteropolymer horse spleen HoSF protein in which subpopulations of HoSF
molecules with varying iron content are observed. A binomial probability distribution of H- and Lsubunits among protein shells qualitatively accounts for the observed subpopulations. The addition
of Fe2+ to apoHuHF produces iron core particle size diameters from 3.8 ± 0.3 to 6.2 ± 0.3 nm.
Diameters from 3.4 ± 0.6 to 6.5 ± 0.6 nm are obtained with natural HoSF after sucrose gradient
fractionation. The change in the sedimentation coefficient as iron accumulates in ferritin suggests
that the protein shell contracts ~10% to a more compact structure, a finding consistent with
published electron micrographs. The physicochemical parameters for apoHoSF (15%/85% H/L
subunits) are M = 484,120 g/mol, ν̄ = 0.735mL/g, s20,w = 17.0 S and D20,W = 3.21 × 10−7 cm2/s;
and for apoHuHF M = 506,266 g/mol, ν̄ = 0.724 mL/g, s20,w = 18.3 S and D20,w = 3.18 × 10−7
cm2/s.
Significance—The methods presented here should prove useful in the synthesis of size
controlled nanoparticles of other minerals.
© 2010 Elsevier B.V. All rights reserved.
*
Corresponding author: Department of Chemistry, University of New Hampshire, Durham, NH 03824., Phone: 603-862-2520, FAX:
603-862-4278, ndc@unh.edu.
§Deceased. This paper is dedicated to the memory of John K. Grady, whose master’s thesis represents part of the work presented here.
He is missed by all who knew him.
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May et al.
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1. Introduction
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Ferritin was first isolated from liver and spleen and crystallized by Laufberger in 1937 and
identified as a protein containing unusually large amounts of ferric iron [1]. Soon thereafter,
Granick and coworkers in a series of papers reported on the iron binding and magnetic
properties of ferritin [2–4]. In the decades following this early work, ferritins have become
one of the most investigated and widespread classes of proteins known. They are found in
cells of nearly all animal tissues and in plants, fungi and bacteria [5–12]. A search in
SciFinder Scholar results in over 30,000 articles related to ferritin. All canonical ferritins
share the same basic structural features, an approximately spherical protein shell of Mr
~500,000 composed of 24 similar or identical subunits arranged symmetrically with 4:3:2
cubic symmetry about a hollow interior which encapsulates large amounts of iron in the
form of a hydrous ferric oxide mineral resembling ferrihydrite [13], the structure of which is
much debated [14–19]. In addition to ferrihydrite, iron oxide phases corresponding to
hematite, magnetite/maghemite and wüstite have also been observed in lesser amounts as
single phases in individual protein molecules [20,21].
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In recent years there has been considerable interest in understanding the
superparamagnetism, ferromagnetism, Mössbauer and quantum tunneling properties of the
nanoparticle iron core of ferritin as well as the mechanism of water proton relaxation
enhancement in magnetic resonance images of the iron laden tissues with high
concentrations of ferritin and hemosiderin [23–29]. Additionally, the protein cage has
proven useful as a reaction vessel, serving as a 3-D template for the synthesis of
nanoparticles of magnetite/maghemite (e.g.[30]), various iron salts [31] and minerals of
other metals such as gadolinium, lead, cadmium, nickel, cobalt, chromium and gold with
interesting and useful properties [32–36]. Ferritin is increasingly finding use in
nanotechnology as discussed in other chapters of this volume. Control or knowledge of the
size of the nanoparticles within ferritin is important in many studies and applications. For
example, uniform iron core sizes with a standard deviation within ± 1 nm are needed for the
fabrication of memory devices [37]. In the case of metallic cobalt cores of ferritin, self
capacitance is directly proportional to the diameter of the core particle [38] whereas the
superparamagnetic relaxation time τs of the ferrihydrite nanoparticles depends exponentially
on the volume of the particle [24,27,28].
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While it is evident that good particle size control is desirable in most applications, this is not
always easily achieved. The capacity of the protein cage in principle limits the maximum
size of the nanoparticle but, in the case of iron, it is usually difficult to fill the cavity to
capacity in vitro. Partially filled ferritin 24mer shells contain cores of varying size about
some average which reflects the overall iron content of the protein. The subunit composition
of the ferritin used strongly influences the size distribution which is obtained. Mammalian
ferritins are composed of two types of subunits, H and L, with Mr ~ 21,000 and 20,000,
respectively, which are structurally interchangeable within in the protein shell. Because
these subunits have different functions, the H-subunit containing a ferroxidase site [39,40]
and the L-subunit mineral nucleation sites [41], the protein shell is inherently polydisperse
in subunit composition, resulting in polydisperse iron mineral cores as well (vide infra).
The mineral core is formed by three iron oxidation reactions [42] which further contribute to
(1)
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(2)
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(3)
its polydispersity in size. Depending upon how iron is added to the protein and the subunit
composition of the ferritin, one or more of these reactions may dominate. When iron is
added in small increments (low-flux ~ 50 Fe per addition), two irons are oxidized per O2 at
the ferroxidase center through the ferroxidase reaction [Eq. (1)]. When a large flux of iron is
presented to the protein (1000 Fe per addition), the reaction largely occurs on the mineral
surface and the stoichiometry of the reaction approaches 4 Fe(II) oxidized per O2 consumed
[Eq. (3)]. At intermediate fluxes, the H2O2 produced at the ferroxidase sites [Eq. (1)]
oxidizes further Fe(II) by the detoxification reaction [Eq. (2)]. Each of these reactions is
important under different experimental conditions of iron loading the protein. Essentially
identical mineral cores are produced with either O2 or H2O2 as the oxidant [(Eqs. (1) and (3)
vs. Eq. (2)] under the same flux of iron into the protein (50 Fe/shell per addition) as
demonstrated by Mössbauer spectroscopy [24].
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Ferritins isolated from tissue or apoferritins reconstituted in vitro show that a significant
fraction (10 – 30 %) of the sample remains relatively iron-free while another fraction is
more fully loaded (approaching 4500 Fe/shell) [43–46]. The basis for these unusual iron
distributions has been uncertain. Mineral growth models have related oxidation through Eq.
(3) to the surface area of the growing mineral [44]. As the core grows in size, additional sites
for oxidation and mineralization are created, allowing for increased capacity for deposition
of additional iron by an autocatalytic mechanism. Under this scenario, those ferritin
molecules that were able to first form a small core would have a greater likelihood of
acquiring more iron than proteins that had not, leaving the latter proteins largely iron free.
However, this model excludes any role for the protein in iron oxidation other than, perhaps,
the formation of the initial iron cluster. An oxidation pathway directed purely by the
ferroxidase site would seemingly allow for more uniform distribution of core sizes because
all iron would be oxidized by the enzyme site and, therefore, in the same manner at the same
rate. Such would be the case if all the protein shells in heteropolymer mammalian ferritins
had the same number of H-subunits, i.e. they were uniformly distributed among the protein
shells, which is not the case [5].
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Analytical ultracentrifugation is well suited for studies of the heterogeneity of ferritin. The
first detailed study was reported by Rothen in 1944 in his seminal work on horse, dog and
human ferritins [46]. While the instrumentation available then was primitive compared to
that today, the main conclusions of Rothen’s work still hold. Apoferritins were observed to
be relatively homogeneous whereas iron containing horse spleen ferritin exhibited marked
heterogeneity and broad sedimentation patterns in which both apoferritin (or low iron
ferritin) and ferritin components were present.
Rothen [46] made the first molecular weight determination of apoferritin using
sedimentation velocity measurements. The molar mass was calculated from the Svedberg
equation [Eq. (4)]
(4)
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where s, R, D, ν̄ and ρ are the sedimentation coefficient, the gas constant, the diffusion
constant, the partial specific volume of the protein and the density of the solution,
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in
respectively. The sedimentation coefficient of apoHoSF was determined to be
corresponds to the sedimentation coefficient in pure water at
Svedberg units (10−13s).
20 °C extrapolated to infinite dilution.) Rothen measured the diffusion coefficient
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cm2/s using a Tiselius apparatus and the partial specific volume
cm3/g of the protein using a quartz pycnometer. Given
g/cm3 for pure water, he
calculated a molecular weight of 467,000. In 1973, Crichton and coworkers reported a value
of M = 443,000 based on their measured value of
S for the apoprotein and using
a value of
mL/g from the amino acid composition known at that time [47]. A
more recent value of Mr ~ 450,000 by sedimentation equilibrium measurements has been
reported [48]. Weight average molecular weights of 460,000, 480,000 and 515,000 have
been published for horse spleen, liver and heart ferritins from sedimentation velocity
measurements [49]. Other values reported for the horse spleen protein have ranged from
430,000 to 480,000 and are summarized in [50]. However, from the molecular weights of
19,978 and 21,269 from the cDNA sequences of the H- and L-subunits, respectively, a
heteropolymer of 15% H and 85% L-subunit (a common composition of the horse spleen
protein) would have a molecular weight of 484,120 only slightly larger than the molecular
weight of 479,472 for the L-chain homopolymer and larger than the values predicted by the
above referenced centrifugation measurements.
The Stokes diameter d of spherical particle (of which ferritin is a good example) is related to
the diffusion coefficient D and viscosity of the solvent (η = 0.01002 poise for pure water at
20 °C) through the Stokes-Einstein equation [Eq. (5)] where kB = 1.3807 × 10−16 erg-K−1 is
the
(5)
Boltzmann constant and T the absolute temperature. D has been measured for horse spleen
ferritin and apoferritin by a number of other methods, including ultracentrifugation [49,51],
dynamic light scattering (DLS) [52], flow field flow fractionation (Fl-FFF) [53] and quasielastic neutron scattering (QENS) [54] under various solution conditions (buffer, salt, etc.),
giving values of D ranging from 2.9 × 10−7 to 3.7 × 10−7 cm2/s. From the Rothen’s value of
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cm2/s for apoHoSF in pure water at 20 °C, a Stokes diameter of an
equivalent hard sphere of 11.9 nm is calculated from Eq. (5), which compares favorably
with the nominally 12.5 nm diameter of the shell of horse spleen apoferritin from its x-ray
crystal structure [55].
Historically, analytical ultracentrifugation has been used in studies of the heterogeneity and
assembly properties of canonical ferritins [43–51,53,56–59] and, more recently, of Dps
proteins [60–62]. AUC is well suited for examining the distribution of mineral core size in
ferritin samples as well as detecting protein cross-linked species and measuring their
hydrodynamic properties. During iron deposition, oxidative damage to the protein leads to
minor amounts of dimers, trimers, tetramers and pentamers with sedimentation coefficients
significantly larger than that of the monomeric protein [53,56,57,59]. Ferritin aggregates
composed of two, three and four protein shells in clusters as well as chain-like assemblies of
a dozen or so shells have been observed in transmission electron micrographs [63].
In the present study, we have employed analytical ultracentrifugation to study the
heterogeneity of ferritin with regard to the polydispersity of the mineral core. The goals of
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the work were to develop a quantitative model for describing the observed sedimentation
coefficient as a function of iron content of the protein and to evaluate methods for obtaining
nanoparticles of reasonably uniform size. Sedimentation velocity measurements were used
to monitor the iron distributions of native horse spleen ferritin and horse spleen and human
H-chain ferritins reconstituted from the apoprotein under different conditions. Ferritin is
unusual, and perhaps unique, in that as it acquires iron within its shell, its overall physical
size and spherical shape remain relatively unchanged while its buoyant mass increases
nearly two-fold. Hence, changes in sedimentation velocity patterns correlate directly with
changes in the distribution of iron loading in ferritin, and can provide insights into the
process of core mineralization within the protein as well as a measure of the degree of
homogeneity of core size within a given sample preparation. Equations are developed for
describing the effect of the change in iron content of the protein on the observed
sedimentation coefficient and for assessing the distribution of core sizes from a linewidth
analysis of g(s)* vs. s* curves. The data suggests that the protein shell is not rigid but
contracts to some extent during core mineralization. Methods of addition of iron to the
protein are assessed and procedures for improving core size homogeneity utilizing sucrosegradient preparative ultracentrifugation are presented.
2. Materials and methods
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2.1 Protein purification
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Recombinant human liver ferritin (HuHF) was isolated from E. coli following a protocol
similar to that described elsewhere [64]. Cells were lysed using a French press at pressures
exceeding 10,000 psi. The lysate was centrifuged at 4 °C for 15 minutes at 14,400 × g to
remove the cellular components. A heat precipitation step at 60 °C for 10 minutes was
followed by another centrifugation step for 20 minutes at 4 °C at 14,400 × g. The
supernatant was collected and brought to 80% saturation with 523 mg/mL (NH4)2SO4. The
suspension was allowed to stir for 2–3 hours at 4 °C and the precipitate was collected by
centrifugation for 20 minutes at 4 °C and 3300 × g. Ferritin was resuspended in a minimal
amount of 25 mM Tris, pH 7.4 buffer and dialyzed for 8 hrs. The protein solution was then
treated with 1 mg/mL DNase, 1 mg/mL RNase and 100 mM MgCl2 at 37 °C for 30 minutes
to remove any contaminating nucleic acids. This mixture was centrifuged to remove any
particulates, and the supernatant concentrated by ultrafiltration using a 100,000 MW cut-off
Millipore membrane. The ferritin solution was run on a Superdex 200 or Sepharose CL-6B
size-exclusion column equilibrated with 0.15 M NaCl, 25 mM Tris, pH 7.4, to remove any
low molecular weight contaminating proteins. Fractions containing HuHF were collected,
combined and run over a Q sepharose, fast flow Pharmacia anion exchange column with a
150 to 500 mM NaCl gradient. Peak fractions were collected, pooled, and dialyzed into
buffer suitable for analytical ultracentrifugation.
Horse spleen ferritin (HoSF) was purchased from Calbiochem (San Diego, CA), MP
Biomedicals (Irvine, CA) and Sigma (St. Louis, MO) and also isolated directly from horse
spleen as follows: The tissue was blended in 1.5× its volume/weight (mL double deionized
water/gram of tissue) and the homogenate subjected to heat denaturation at 70 °C for 10
minutes followed by centrifugation at 3300 × g for 30 minutes at 4 °C on an IEC MP4R
centrifuge employing a swinging bucket rotor. After a second centrifugation step at 14,400 ×
g with a fixed angle rotor (IEC #854), β-mercaptoethanol to a concentration of 1 mM was
added to the red-brown supernatant. The ferritin was precipitated with ~50% saturated
(NH4)2SO4 (313 mg/mL) with overnight stirring at 4 °C. The pellet was resolubilized in 20
mM sodium phosphate, pH 6.8 with stirring for 1 hour at 4 °C. Low molecular weight
contaminants were removed by size-exclusion chromatography using a Superdex 200, 50 cm
column. If necessary, the (NH4)2SO4 step was repeated as needed to obtain a homogeneous
ferritin solution as judged by size exclusion chromatography and PAGE. All isolated
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ferritins were run on a 15% SDS-PAGE and a 7% native PAGE gels to assess protein purity
and to verify the absence of significant cross-linking between protein shells. The subunit
composition of the HoSF used in this work was determined to be 15% H and 85% L by
SDS-capillary gel electrophoresis as described [65].
2.2 Iron analysis
The iron content of native and reconstituted ferritins was measured by a modified
colorimetric ferrozine assay [66]. Samples of known protein concentration were incubated at
90 − 95 °C in 10% trichloroacetic acid for 2 hours in 1 mL total volume followed by 10 min
on ice. Denatured protein was removed by centrifugation. An aliquot of the supernatant was
added to 100 µl of saturated ammonium acetate, 62.5 µl of 0.12 M ascorbic acid and 62.5 µl
of 0.25 M ferrozine [3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p, p’-disulfonic acid,
monosodium salt hydrate]. Samples were incubated at room temperature for 30 minutes.
Absorbance at 562 nm was measured (molar absorptivity of the Fe complex at 562 nm =
27,900 M−1 cm−1) and the ratio of Fe to protein calculated.
2.3 Preparation of apoferritins
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Ferritins, as purchased or isolated, contain varying amounts of iron. The HoSF generally
was received or isolated with an average of ~ 2000 − 2600 Fe/shell whereas HuHF usually
contained ≤ 50 Fe/shell. Iron was stripped from the protein by anaerobic dialysis using
dithionite as the reductant [67]. Horse spleen ferritin was subjected to 5 changes with a
~50:1 volume ratio and 6 hours between changes against an anaerobic solution of 0.1 M
sodium acetate, pH 5.2 to which dithionite had been added to a concentration of 3 g/100 mL.
The procedure was repeated with the dithionite concentration reduced to 0.3 g/100 mL for
the second cycle. A final anaerobic cycle of 5 changes was performed in the absence of
dithionite with 1 mM of 2,2'-dipyridyl added to scavenge any remaining iron. Exhaustive
dialysis to remove dipyridyl was performed using 10 mM Mops, 0.15 M NaCl, pH 7.1
(several changes). In the case of the more fragile HuHF, 50 mM MES, pH 6.0 was employed
in place of acetate buffer [67]. The apoferritins were assayed for concentration either by UV
(ε280 = 19,500 M−1 cm−1 subunit−1 for HoSF; ε280 = 22,700 M−1 cm−1 subunit−1 for
HuHF) or using the BioRad Protein [68] and Better Bradford (Pierce Biotechnology,
Rockland, IL, USA) assays. The Better Bradford assay was employed in all experiments
reporting Fe/shell ratios because the presence of iron does not interfere with this method of
determining protein concentration whereas the other assays showed some level of
interference.
2.4 Reconstitution of ferritins
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Ferritin was reconstituted by aerobic addition of Fe(II) to apoferritin (0.25 or 1.0 mg/mL)
using either high (500 or 1000 Fe(II)/addition) or low (50 Fe/addition) flux conditions.
Ferritins were reconstituted to final iron loadings of 500, 1000, 1500, 2000 or 2500 Fe/shell
in both low and high iron fluxes or to a level where precipitation was observed. For the low
iron flux samples, a series of additions at 50 Fe(II)/shell were made, allowing at least 10
minutes between additions for the complete oxidation of Fe(II) [69]. The 1 mL ferritin
solutions were open to air and constantly stirred to maintain molecular oxygen in the
sample. The high iron flux samples were made using a maximal single addition of 1000
Fe(II)/shell at a protein concentration of 0.25 mg/mL to keep the sample from going anoxic
during the course of the experiment. Under these conditions about 50% of the dissolved O2
would be consumed based on the known oxidation stoichiometry of 4 Fe(II)/O2 [69,70].
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2.5 Density and viscosity measurements
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The density of the 0.1 M MOPS, 0.15 M NaCl, pH 7.5 buffer was measured at 20 °C with an
Anton Paar DMA 02D densitometer, standardized with pure water and dry air. The dynamic
viscosity was measured with an Anton Paar AMVn automated micro viscometer relative to
air-saturated water at 20 °C.
2.6. Preparation of Sucrose Gradients
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Sucrose solutions were made (w/w) in 0.15 M NaCl. Sucrose gradients were prepared using
a two layer method (http://web-mcb.agr.ehime-u.ac.jp/english/methods/gradient.htm) in
which 8 mL of 50, 60 or 70% sucrose was added to the bottom of a 16 × 22 mm
ultracentrifuge tube (Beckman, 344061) and 8 mL of 0% sucrose solution gently layered on
top. Tubes were covered tightly with parafilm and then slowly turned horizontal for 12 hours
and then back to a vertical position. Gradients were also prepared by layering 4.8 and 23%
(w/w) sucrose solutions (equivalent to 5 and 30% w/v) for 15 min, 30 min, 1 hr or overnight.
Protein samples were gently layered on top of the gradient and spun on a Sorvall Ultra 80
centrifuge fitted with the AH-629 rotor at 25000 rpm (80,000 × g) at 4 °C for 8.75 hr with
no braking. After centrifugation, sequential 1.5 ml fractions were taken from the top of the
sucrose gradient and the percentage sucrose of each fraction measured with a Misco PA202
Palm Abbe digital refractometer to verify the integrity of the gradient. Each fraction was
also analyzed for iron and protein. Fractions were then dialyzed into 0.1 M MOPS, 0.15 M
NaCl, pH 7.5 buffer for the AUC measurements.
2.7. Sedimentation velocity experiments and analysis
Sedimentation velocity studies were carried out in 0.15 M NaCl, 0.1 M MOPS, pH 7.5, at
24,000 rpm and 20 °C on a Beckman Optima XLI analytical ultracentrifuge using Rayleigh
interference optics, 12-mm double sector charcoal-epon centerpieces, and either a 4- or 8hole titanium rotor, with ~8 seconds/scan. The apparent sedimentation coefficient
distribution, g(s*) = dc/ds*, was calculated from the time derivative of the concentration
profile with DCDT+ (uncorrected for the effects of diffusion) [71,72]. Although there are
many other approaches available for analyzing sedimentation data (e.g. [73]), DCDT+ was
determined to be most suited for the heterogeneous samples and broad sedimentation
distributions encountered in this work. Because it is model independent, post analysis of the
linewidths of the g(s*) curves can be carried out to obtain the dispersion in core size for
different samples (vide infra). In all instances, care was taken to choose a sufficiently
narrow time span of the data so as to avoid artificial boundary spreading.
2.8. Effect of iron loading of ferritin on its sedimentation coefficient
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The partial specific volume of apo-HoSF was originally reported as ν̄protein = 0.747 mL/g
[46] but a more recent refined value is 0.738 mL/g [74]. A value of ν̄protein = 0.735 ± 0.007
mL/g was used in this work as calculated using Sednterp [75] based on the amino acid
composition of a protein of 15% H and 85% L subunits. The H and L homopolymers have
Sednterp calculated ν̄protein values of 0.727 and 0.736 mL/g, respectively. The partial
specific volume ν̄complex and, consequently, the sedimentation coefficient of the iron-ferritin
micellar complex will change as iron is accumulated within the protein. The effect of iron
loading on ν̄complex can be estimated from equation (6) where gprotein is the gram mass of the
protein;
(6)
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ν̄protein = 0.735 mL/g; gcore is the gram mass of the accumulated iron core; ν̄core is the
specific volume of the iron core; and gtotal is the gram mass of the protein plus its
accumulated core.
The value of ν̄core was estimated from published density measurements of ferrihydrite
nanoparticles of various sizes ranging from 2.6 to 7.4 nm and containing from ~ 100 to ~
3000 Fe per cluster [14]. A linear regression of the specific volume of the mineral as a
function of cluster size for the larger iron clusters of nFe = 370 to 3000 Fe results in a
reasonable fit (R= −0.991, N=3), giving ν̄core = −7.7 × 10−6nFe + 0.286 mL/g. Thus, ν̄core
ranges in value from ~0.29 mL/g for small iron clusters to ~ 0.25 mL/g for a fully loaded
ferritin of 4500 Fe/shell.
The expected sedimentation coefficient of the complex in 0.1 M MOPS, 0.15 M NaCl, pH
7.5 is given by Eq. (7):
(7)
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where Mprotein = 484,120 g/mol is the molar mass of the HoSF used in the present study; nFe
is the number of iron atoms in the core based on the reduced formula of ferrihydrite,
Fe5O12H9 [14]; Mcore = 481 g/mol is the molar mass of the ferrihydrite unit cell; ν̄complex is
the partial specific volume of the protein/iron complex, calculated as described above; ρ =
1.0121 ± 0.0001 g/mL is the measured density of the 0.1 M MOPS, 0.15 M NaCl, pH 7.5
buffer; NA is Avogadro’s number; and f = 1.34 × 10−7 g/s is the frictional coefficient of the
protein calculated from the rearranged Svedberg equation [Eq. (8)] using the average value
of sprotein = (15.4 ±
(8)
0.6) × 10−13 s for the apoferritin (N = 7) in 0.10 M MOPS, 0.15 M NaCl, pH 7.5 (vide
infra). In the model, the frictional coefficient is assumed unchanged by the presence of the
mineral core.
By combining equation (6), (7) and (8) and setting gprotein = Mprotein and gcore = nFeMcore/5,
a final expression relating
to nFe is obtained [Eq. (9)].
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(9)
2.9. Correction of g(s*) as a function of iron loading
Sedimentation velocity data were corrected for the change in refractive increment with
concentration (dn/dc) as iron is loaded into the protein. The values for (dn/dc)protein and (dn/
dc)core are 0.00173 and 0.00218 mL/g, respectively [46]. Under the assumption that dn/dc
can be expressed as a weight average, a weighting function w(s) was applied to the g(s*)
data to account for changes in refractive increment due to iron [Eqs. (10) and (11)]:
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(10)
and
(11)
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where c is in g/cm3, gprotein = Mprotein and gcore = nFeMcore/5 and F(s) is a function relating
g(s*) to the fractional amount of iron loading that accounts for the increase in dn/dc due to
iron. Therefore, g(s*)corrected = g(s*)raw · F(s) with F(s) ranging from 1.0 for apoferritin to
0.891 for ferritin having 4500 Fe/shell. Computed values of F(s) fit very well to a decaying
exponential: F(s) = 0.137 · e (−nFe/2883) + 0.863, R = 0.9999 where nFe = (86.9±3.0) ·
scomplex −(1339±70) for HoSF and nFe = (86.9±3.0) · scomplex −(1443 ± 70) for HuHF (see
below). Application of the F(s) correction to the g(s*)raw data affects the integrated areas
under the peaks by 5% or less for the samples in this work which typically contain ≤ 2500
Fe/shell.
2.10. Linewidth analysis
Individual peaks in the g(s*) vs. s* plots were fit to a Gaussian function of the form
, the area
under the peak being proportional to
the concentration of species present. The observed linewidth σobs has contributions from
. σshell was determined from
both the protein shell and the mineral core, i.e.
curve fitting of the peak for the apoprotein i.e. σobs = σshell, enabling σcore to be calculated
from σobs measurements of iron containing samples. However, σshell itself has contributions
from both protein heterogeneity and diffusion of the protein, namely
.
Because σdiff can be calculated, the homogeneity of the protein can then be assessed from its
σhetero value. The diffusion contribution to the linewidth was computed from the
NIH-PA Author Manuscript
relationship
where the diffusion coefficient D corresponds to that of the
protein in buffer (D = 2.99 × 10−7 cm2/s for apoHuHF and D = 3.01 × 10−7 cm2/s for
apoHoSF), t is the sedimentation time, rm is the radius of the meniscus and ω is the angular
velocity of the rotor. Because t and ω2 t are different for each scan in the series used to
compute g(s*) f or a given sample, their average values were used for the calculation of σdiff
[72]. Different sets of scans of narrow time range were used for calculation of g(s*) for
different samples and therefore the computed value of σdiff depend on the particular
sedimentation run as well as on the identity of the sample.
3. Results
3.1. Sample calculation
The calculated scomplex as a function of iron loading is shown in Fig. 1 (curve a). The
predicted values of scomplex from equation (9) are remarkably linear with iron loading within
the thickness of the line (linear regression: scomplex = 0.00882 · nFe +15.4, R = 0.999; or nFe
=113.4 · scomplex −1746 ). Curve b is an empirical plot of the weighted average sW of the
sedimentation coefficient calculated by Eq. (12) as a function of nFe for a series of HoSF
samples
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NIH-PA Author Manuscript
(12)
obtained by preparative centrifugation on a sucrose gradient (linear regression: scomplex =
(0.0115± 0.0004) · nFe+15.4 ± 0.6; or nFe =(86.9 ± 3.0) · scomplex −(1339 ± 70). The iron
contents of the samples were measured by the ferrozine method and the protein
concentration by the Better Bradford assay (Materials and Methods). The slopes of predicted
curve a and experimental curve b differ by ~23%. This difference cannot be reconciled by
any reasonable choice for the values of Mcore and ν̄core of ferrihydrite used in Eq. (9) [14–
19], nor by using the corresponding values for the other minerals present in lesser amounts
in ferritin cores [20,21]. These findings imply that the assumption inherent in Eq. (9) that the
frictional coefficient of the protein is constant is not valid. Rather f appears to decrease to
some extent as iron is accumulated within the protein shell, resulting in a further increase in
s than that predicted on the basis of the buoyant mass of the core alone (more later). In
subsequent analyses, we employed the empirical relationship between scomplex and nFe
represented by the least-squares line of curve b in Fig. 1.
NIH-PA Author Manuscript
The y-intercept of curves a and b in Fig. 1 corresponds to the experimentally determined,
uncorrected s = 15.4 S for apo-HoSF. Correction of s according to Eq. (13) for measured
(13)
NIH-PA Author Manuscript
buffer density and viscosity at 20 °C (ρ*= 1.0121 ± 0.0001 g/mL, η *= 0.01067 ± 0.00003
poise) to that of pure water at 20 °C ( ρ20,w = 0.99823 g/mL, η20,w = 0.01002 poise) with ν̄ *
= ν̄20,w = 0.735 ± 0007 mL/g gives s20,w = 17.0 ± 0.6 S (N = 7, 95% confidence interval).
This value compares with previously published values of s20,w = 17.9, 17.6, 17.1, 16.6 and
16.5 S [46–49,74]. The relatively large error of ± 0.6 S associated with our value of s20,w
reflects the heterogeneity in the apoprotein and likely accounts for the range of values
reported in the literature. In our work, s values ranged from 14.3 to 16.7 S for various
apoprotein samples prepared from commercially supplied horse spleen ferritin and from
protein isolated in our laboratory. This large range reflects oxidative damage to the protein,
the tendency of the protein to cross-link to form dimers and higher order oligomers and the
inherent heterogeneity associated with the variable subunit composition of the protein itself
[49,53,56,57,59,65,76–78]. From s20,w= 17.0 S, M = 484,120 g/mol and R = 8.3145 × 107
erg-K−1mol−1, we calculate through
(14)
Eq. (14) a translational diffusion coefficient of D20,w = (3.21 ± 0.15) × 10−7 cm2/s which
compares well with the “best” published value of D20,w = 3.24 × 10−7 cm2/s for apoHoSF
[51,74]. From D20,w = (3.21 ± 0.15) × 10−7 cm2/s, we calculate a Stokes diameter of 13.3
nm for the apoprotein (Table 1).
3.2. Examination of horse spleen ferritins from different sources
The sedimentation patterns of horse spleen ferritin from four sources are compared in Fig. 2.
While they differ markedly from one another, they all show a largely iron free fraction and a
heterogeneous iron containing fraction as originally observed by Rothen [46] and later by
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others [43,44]. The average Fe/shell ratio of each of the samples can be estimated from
equation (15)
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(15)
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where nFe(s*) is given by the linear relationship nFe = 86.9 · scomplex −1339 from the fit of
curve b in Fig. 1. Substitution of sW into the equation nFe,ave = 86.9 · sW −1339 is
mathematically equivalent to Eq. (15) and more convenient for calculations. Acceptable
agreement is obtained between the iron contents nFe from AUC, which has an estimated
relative standard deviation of ± 10% and those from ferrozine and Better Bradford analyses
with measured relative standard deviation of ± 3.8% (Table 2), validating the use of Eq. (15)
for determining the iron content of samples. We note that the presence of some
intermolecular cross-linking would bias sW to a higher value and would thus produce an
artificially high result for nFe,ave. However, native PAGE showed no visual evidence of
oligomeric species in any of the four HoSF samples in Table 2, indicating that cross-linked
protein must represent less than ~ 5% of the total present and thus would not unduly affect
the value of nFe from Eq. (15). However, some cross-linking is present as discussed below
and, if desired, dimers, trimers and higher oligomers can be removed using size exclusion
chromatography [79].
3.3. Comparison of HoSF reconstituted at high and low iron flux
Solutions of apoHoSF and reconstituted HoSF containing an average of 500 to 2000 added
Fe/shell were subjected to sedimentation velocity analysis to determine the distribution of
core sizes. The apoHoSF sample yields a main peak centered at 14.4 S, with a small amount
(~ 3%) of dimer at 24.9 S, the precise position being determined from a Gaussian fit (Fig. 3,
black solid line). The dimer is predicted to occur at s2 ~ 24.4 S based on the relationship s2/
s1= (M2 / M1)2/3 for same shaped molecules and using the average s1 = 15.4 S for the
monomer (vide supra). This type of sedimentation pattern has been reported previously for
solutions of apoHoSF in which the higher molecular weight species at ~ 24 S have been
determined to be apoHoSF dimers [56,57,59]. The Gaussian linewidth σobs = σshell = 6.5 G
for the monomeric species in Fig. 3 is significantly larger than the calculated linewidth due
to diffusion, σdiff = 2.8 S. Consequently the observed linewidth is primarily due to structural
heterogeneity in the chemically prepared apoprotein itself, namely
S (Materials and methods).
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HoSF was reconstituted from apoHoSF by addition of 2000 iron under either low- or highflux conditions (Fig. 3). The protein was exposed to 2000 Fe/shell by either a single addition
of 40 µL of 27 mM FeSO4 in 0.01 M HCl to 1 mL of 0.25 mg/mL protein sample (red
dotted line) or a series of forty 1 µL additions (green dashed line). The sedimentation
coefficient distributions for low- and high-flux HoSF samples containing an average of 2000
Fe per protein are very different, indicating that the iron core size distributions are different.
High-flux conditions result in a distribution that consists of a single peak at ~ 18 S which
trails off slowly to higher s. In our experience the overall shape of the distribution is
reproducible but minor features are not reproducible for different sample preparations. The
irreproducibility of the distribution details suggests that the exact size distribution of the iron
cores depends on the particulars of the sample and of its handling, even though every
attempt was made to prepare the samples identically. Given this variability in minor features
in the sedimentation pattern from sample to sample, there is little advantage in using a
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potentially higher resolution analysis, especially because oscillations in the pattern can result
which could be erroneously interpreted as peaks [73].
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The bimodal character of the sedimentation distribution for HoSF loaded under low-flux
conditions is strikingly different from the high-flux distribution. For the low-flux sample,
there appears to remain a significant amount of iron poor or apo-HoSF (~15 S) in addition to
a broad distribution of HoSF centered ~ 45 S (~2550 Fe/shell). The sW values for the highand low-flux samples are 30.4 and 37.0 S, corresponding to an average iron content from
Eq. (15) of 1290 Fe/shell and 1860 Fe/shell, respectively, compared to the 2000 Fe/shell
added. Thus more effective loading of the apoprotein with iron occurs under the low-flux
conditions.
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The results of additional iron loading experiments employing 500, 1000, 1500, 2000 and
2500 Fe/shell further reveal that very different iron core distributions develop depending on
how apoHoSF is exposed to iron (Fig. 4). Under high-flux loading conditions (Fig. 4A),
there is a progressive broadening of the distribution, until the distribution is nearly uniform
over the range from ~ 20 to ~ 60 S (magenta dashed curve), and there is no distinct ironpoor fraction remaining, indicating essentially a continuum of iron containing species are
present. sW values range from 20.0 S for the 500 Fe sample to 40.6 S for the 2500 Fe
sample, corresponding to iron contents nFe from AUC of 395 to 2175 Fe/shell, respectively.
However, when HoSF is exposed to iron under low-flux conditions (Fig. 4B), there is a
persistent peak in the distribution corresponding to apo-HoSF (~ 15 S), much like the
holoprotein samples of Fig. 2, with a second peak that develops at iron loading levels
exceeding 1500 Fe/shell. The iron-rich peak shifts to higher s with increasing levels of iron
with peaks at speak ~ 35, 42 and 60 S for 1500, 2000 and 2500 Fe/shell, respectively. sW
values range from 21.6 S to 39.0 S, corresponding to nFe values from AUC ranging from
540 to 2030 Fe/shell for the 500 to 2500 Fe/shell samples. Additionally, both the low-flux
and high-flux samples show the presence of a number of subpopulations within the ironcontaining fractions.
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Under low-flux conditions, samples loaded to 2500 Fe/shell exhibited a small amount of
precipitate. Under the conditions of these experiments, precipitated proteins will pellet prior
to the onset of data acquisition. In order to determine the amount of protein lost to
precipitation, integrals of the g(s*) curves were taken (Table 3). While no precipitation was
observed by eye in samples with an iron loading of 2000 Fe/shell or less, it is clear from the
integrals that loss of protein becomes significant at levels of 1500 Fe/shell and higher; i.e.,
in most instances there is a significant reduction in the relative values of the integrals
compared to those of samples containing less iron. Precipitation of both protein and iron
likely contributes to the observed differences in the expected Fe/shell ratios and those
determined by AUC on spun samples.
3.4. Human H-chain (H24L0) ferritin
The inherent heterogeneity of HoSF shells with respect to their H- and L-subunit content
likely contribute to the differences observed in iron deposition under low- and high-flux
conditions and to subpopulations within the iron-containing fractions (Figs 3 and 4).
Therefore, iron-distribution analyses were conducted using recombinant human H-chain
ferritin homopolymer (HuHF, H24L0). The sedimentation profile of apoHuHF shows some
dimerization of the protein (Fig. 5) as evident from the shoulder appearing at ~ 24 S.
Treatment of the protein with dithiothreitol (DTT) reduces the amount of aggregated protein
from 54% to 13% (curve a vs. curve c) as determined from fitting the respective g(s*) curves
to a sum of two Gaussians and determining their relative areas. Dimerization of the HuHF
through disulfide cross-links from oxidized Cys90 and to a lesser extent from dityrosine
formation has been well documented [77,78]. The treated apoprotein sample can be curve fit
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to a sum of two Gaussians functions with fitting parameters for the monomer of speak =
16.59 ±0.02 S (σobs = σshell = 2.7 S) and for the dimer 23.9 ±0.1 S (σobs = σshell = 2.8 S)
(Fig. 5, inset). The linewidth σshell = 2.7 S for the 5 mM DTT treated apoHuHF monomer is
the same within fitting error as the calculated diffusion limited linewidth σdiff = 2.8 S,
indicating that the DTT treated apoprotein is homogeneous, i.e. σhetero ~ 0. The linewidth of
the untreated monomer is 2.9 S, only marginally larger than that of the treated protein. For
the dimer, σdiff = 2.4 S, which in combination with σobs = σshell = 2.8 S, gives σhetero = 1.4
S, indicating the presence of a small amount of heterogeneity in this oligomer.
S for the monomer and ν̄* = ν̄20/w 0.724 ± 0.007mL/g based
From the value of
on the H-chain amino acid sequence, a value of s20,w= 18.3 ± 0.1 S for apoHuHF is
calculated [Eq. (13)]. A value of D20,w(apoHuHF) = (3.18 ± 0.09) × 10−7 cm2/s is calculated
from Eq. (14) using s20,w(apoHuHF) = 18.3 S, MHuHF = 506,266, ν̄HuHF = 0.724 ± 0.007
mL/g and ρ20,w =0.99823 g/cm3 from which a Stokes diameter of 13.5 nm is obtained [Eq.
(5)]. Similarly, for the dimer, a value of s20,w = 26.4. ± 0.1 S, D20,w = (2.3 ± 0.1) × 10−7
cm2/s and Stokes diameter of d = 18.7 nm are calculated (Table 1).
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Fig. 6 shows the sedimentation velocity profiles of a series of apoHuHF samples to which
100, 500, 1000, 1500 and 2000 Fe/shell were added in 50 Fe/shell increments. Subsequent
ferrozine and protein analyses gave 16, 667, 1908, 2621 and 3042 Fe/shell, respectively,
indicating that, with exception of the first sample, precipitation of protein relative to iron
occurs, resulting in protein enriched in iron and exhibiting higher Fe/shell ratios. The traces
all show considerably improved homogeneity over that seen with HoSF samples (c.f. Figs.
4B and 6); however, both monomer and dimer peaks are evident.
The main peaks of the sedimentation profiles of the five samples were fit to a sum of two
for the monomer and dimer components from curve fitting are
Gaussians. The values of
plotted in Fig. 1 as a function of the iron content of the protein (curves c and d). The ratio of
the slopes of curves c and d is 1.57 in accord with the expected value of 1.59 based on the
22/3 relationship between s values of monomers and dimers. The cores developed in both the
monomer and dimer must have approximately the same amount of iron per protein shell
otherwise the 22/3 relationship would not hold. Furthermore, the parallel lines observed for
HoSF and HuHF (curves b and c) indicate that the core of each contributes the same to the
buoyant mass regardless of whether the core is present naturally as in the HoSF samples or
reconstituted as in the HuHF samples.
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The diameter of a spherical particle is related to its iron content nFe and specific volume
ν̄core through the Eq. (16). Equation (16) and a linewidth analysis of the Gaussian peaks
were
(16)
used to estimate the size and polydispersity of the core. Given that σobs = σshell = σdiff as
noted above for apoHuHF, the value of σcore was calculated, i.e.
, and
related to the standard deviation in the iron content of the core σFe through the relationship
σFe = 86.9 · σcore. The standard deviation in the diameter σd is given by σd/d = σFe/3nFe. An
analysis of the 667, 1908, 2621 and 3042 Fe/shell samples of HuHF gave diameters of 3.84
± 0.32, 5.40 ± 0.18, 5.96 ± 0.22 and 6.21 ± 0.30 nm, indicating that all of the samples have
low dispersion in their core sizes with relative standard deviations ranging from ± 3 to ± 8%
with the larger cores having relatively less dispersion in size.
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Fig. 7 illustrates the difference between high-flux (Fig. 7A) and low-flux (Fig. 7B) samples
for another HuHF protein preparation. Significant precipitates are observed at levels over
~1000 Fe/shell, especially with the low-flux samples which is probably a consequence of
hydrogen peroxide production through equation (1) and subsequent damage to the protein
through Fenton chemistry [80]. These observations are in accord with previous reports that
H-rich ferritins are less efficient at storing large iron cores than L-rich ferritins, resulting in
aggregation and precipitation at higher iron levels [41]. For the high-flux samples, AUC
measurements of iron content of the 500, 1000, 1500 and 2000 Fe/shell samples gave 575,
925, 1195 and 1495 Fe/shell, indicating that additions above 1000 Fe/shell result in less iron
being acquired by the protein. For the low-flux 500, 1000 and 1500/shell samples (Fig. 7B),
iron contents of 585, 990 and 1380 were obtained from AUC analysis. No population of
iron-poor species is observed in Fig. 7 which contrasts with the data for HoSF (Fig. 4).
Instead, the distributions shift to progressively higher s as more iron is loaded. Likewise,
there is little evidence for subpopulations within the peak although some asymmetry is
observed where dimers are expected to be present.
3.5. Sucrose gradients
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Sucrose gradients were prepared to improve the homogeneity in the cores obtained with
HoSF. Sucrose gradients using the two-layer method are illustrated in Fig. 8. Gradients
made with the starting solutions of 0 & 50, 0 & 60 and 0 & 70% w/v sucrose had ranges 12–
33%, 11–43% and 13–52%, respectively (Fig. 8A), as determined by refractive index
measurements employing the standard curve (Fig. 8A, inset). Gradients were also prepared
from solutions of 4.8 & 24% sucrose incubated for 15, 30 and 60 min and overnight,
producing more sigmoidal shaped gradients with ranges of ~ 6.4–23% (Fig. 8B).
3.6. Horse spleen ferritin separated on a sucrose gradient
NIH-PA Author Manuscript
Fig. 9 shows the normalized g(s*) versus s* curves for eleven fractions of commercial HoSF
from MP Biomedicals separated on a 6.4–23% w/w gradient (equivalent to 6.8 to 30% w/v),
a gradient typical of those commonly used to fractionate ferritins. For comparison, g(s*) of
the unfractionated protein is shown as a bold line in Fig. 9 at 90% of the amplitude of the
others. The sedimentation profile observed differs markedly with each fraction and from the
unfractionated protein. The 1.5 mL fractions have the following Fe/shell values determined
by the ferrozine assay with the percentage of the total protein in the fraction given in
parentheses: 1. 29 (10.6); 2. 68 (14.9); 3. 444 (11.0); 4. 1180 (10.3); 5. 1795 (10.6); 6. 2747
(10.8); 7. 3281 (9.5); 8. 2900 (9.1); 9. 3623 (6.0); 10. 3459 (4.0) and 11. 3465 Fe/shell
(3.2%). The heaviest material (nFe > 3600) sedimented to the bottom of the tube. Use of a
steeper gradient of 2.2 to 66.7% w/w resulted in significantly poorer resolution. However,
the fastest sedimenting Gaussian component in fraction 11 of the steeper gradient appeared
at s = 65.1 S, corresponding to nFe = 4320, a value close to the 4500 Fe/shell capacity of the
protein. As expected, the relative order of the maximal s of the peaks in Fig. 9 approximates
the iron contents of the samples. Most significant is the observation that no fraction contains
protein in abundance (> 15%), illustrating the extreme polydispersity of the cores of natural
horse spleen ferritin. Because of the wide range of iron contents among the subpopulations
of HoSF samples, it is difficult to obtain any one component in large yield relative to the
others.
Fraction 1 is largely apoferritin (nFe = 29) and consists of 71% monomer (σobs = 2.3 S), 18%
dimer (σobs = 2.3 S) and 11% trimer (σobs = 4.8 S), the relative amounts of protein being
determined from the areas under the three Gaussians. The hydrodynamic parameters of the
monomer and dimer are summarized in Table 1. The linewidth of the monomer (σobs = σobs
= 2.3 S) is close to that predicted for diffusion alone (σdiff = 2.5 S), indicating a
homogeneous protein which contrasts with the heterogeneity noted above for chemically
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prepared apoprotein. Presumably this difference is due to the fact that the apoprotein
isolated on a sucrose gradient has been exposed to very little iron and associated reactive
oxygen species.
The contribution of σcore to the observed linewidths of the iron containing fractions of Fig. 9
range from σcore = 2.2 to 11.9 S for fractions 3 to 11, corresponding to dispersions in iron
content of the particles ranging from σFe ± 216 to + 1030 Fe/particle. Corresponding particle
sizes range from 3.37 ± 0.55 to 6.50 ± 0.64 nm. Fraction 6 contains 2747 ± 670 Fe/shell (d =
6.04 ± 0.48 nm), which is the fraction closest in iron content to 2745 ± 1040 Fe/shell (d =
6.04 ± 0.76 nm) for the iron containing component at s = 47 of the unfractionated protein
(Fig. 9). Thus, sucrose gradient fractionation resulted in a ~ 40% decrease of in
polydispersity of the core. While a reasonably good separation of HoSF species according to
core size with decreased dispersion was obtained using a sucrose gradient, the dispersion
was still about twice that seen for the HuHF samples with cores of similar iron content.
4. Discussion
4.1. Contraction of the protein shell?
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One of the most interesting observations of the present study is the apparent decrease in the
frictional coefficient f of the protein as the mineral core develops within the cavity. Because
f = 6πηr for a spherical molecule, this finding suggests that the protein shell contracts in size
as it acquires iron. The average iron content of HoSF isolated from spleen is ~ 2300 Fe/shell
(Table 2). According to curve b of Fig. 1, a HoSF molecule containing this amount of iron
sediments at s = 41.8 S (M = 705,380 g/mol, ν̄ = 0.588 mL/g) and has D = 3.58 × 10−7 cm2/s
[Eq. (14)] which corresponds to a Stokes diameter of 12.0 nm or a ~10% reduction over the
value of 13.3 nm for the apoprotein. These findings are consistent with early published TEM
images of shells of apoferritin and ferritin in the same micrograph [63,81] (Fig. 10). From
Fig. 10, the diameter of the apoprotein shell is estimated to be ~ 12.6 nm, close to the Stokes
value of 13.3 nm from this work. More importantly, the size of the protein shell in the
micrograph is seen to decrease by ~ 12% in the presence the iron core. While it is
conceivable that the observed reduction in the protein shell seen in the TEM image may be
simply an artifact of drying of the sample, the fact that a similar reduction is suggested by
the AUC data, and a reduction, albeit smaller, has been observed by DLS [73] warrants
further study. Some contraction of the protein shell could occur through the binding of Fe3+
at glutamate nucleation sites on the inner surface of the protein cavity and at sites within the
hydrophilic channels, reducing charge repulsions and giving rise to a more compact
structure. In this connection, association between the core and protein shell has been seen in
TEM images [63] and x-ray absorption and Mössbauer measurements have shown that iron
in the early stages of development of the mineral core binds to carboxylate groups within the
cavity [82]. In reconstituted HuHF, Fe3+ has been demonstrated to migrate from the twentyfour ferroxidase sites to the eight hydrophilic channels where it binds [83].
4.2. The distribution of iron core sizes is dependent on iron presentation
The data demonstrate that the manner in which iron is presented to apo-HoSF results in
distinct iron distributions in HoSF (Figs. 3 and 4). Under low-flux conditions, in which the
iron is oxidized primarily at the ferroxidase site, a progressive fractionation of the HoSF
sample into iron-poor and iron-rich fractions is observed (Fig. 4B), similar to that seen with
native horse spleen ferritins (Fig. 2). The iron-poor fraction appears to reach a constant level
when the average iron content is about 1500 Fe/shell and accounts for ~15% of the total
protein in the sample. As more iron is added to the sample, the iron-rich fraction appears to
sequester the additional iron preferentially. The iron-rich fraction is a broad distribution
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(from 35 to ~70 S in the 2500 Fe/shell sample) and consists of a number of subpopulations,
as indicated by the unresolved shoulders and humps in the g(s*) distribution (Fig. 4B).
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The high iron flux g(s*) distributions for HoSF (Fig. 4A) are quite different from low flux
distributions (Fig. 4B). Under high-flux conditions the distributions become extremely broad
as iron is loaded to 2500 Fe/shell, extending over the whole range of sedimentation
coefficients accessible to HoSF shells (from 15.6 s to ~70 s). As increasing amounts of iron
are presented to the protein, the center of the distribution shifts to higher s and broadens,
indicating that iron is being loaded to produce a broad distribution of core sizes among the
protein molecules present. This is in sharp contrast to the nearly bimodal distributions
observed with low-flux additions. There is some remaining iron-poor ferritin, but its level is
hardly distinct from the rest of the sample.
4.3. Subpopulations of iron-loaded species in HoSF
The subpopulations seen in the g(s*) vs. s* plots are likely due to different numbers of Hsubunits in each shell of HoSF. The average composition of horse spleen ferritin is generally
stated to be ~ 15% H-subunit and 85% L (~H4L20) as found in this work. However
compositions ranging from 10% (~H2L22) to 20% (~H5L19) have been reported [5,10]. If it
is assumed that ferritin assembly is a stochastic process, the distribution of H and L subunits
within a sample of given fractional population of each subunit is determined by the binomial
NIH-PA Author Manuscript
, where B(x,N,p) is the
probability distribution:
probability of finding a given species having x L-type subunits out of the total, N, of 24
subunits, and p is the fractional population of L subunits in the sample. Separate calculations
in which p was fixed at 0.8, 0.85 and 0.9 (i.e. 80%, 85% and 90% L-subunit) are shown in
Fig. 11A. At least 10 different species are statistically possible for HoSF, assuming an
average H composition of 15%. A dispersion (standard deviation) in molecular weight of ~
2200 is predicted by the distribution in Fig. 11A which corresponds to a contribution of only
0.07 S to the linewidth of the apoprotein. Therefore, heterogeneity associated with subunit
composition of individual ferritin shells is smaller than the precision of ± 0.1 S in the
linewidth measurements.
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The species in Fig. 11A will have vastly different ferroxidase activities. If one assumes that
there is no cooperativity between H or L subunits with respect to the rates of iron oxidation
(which is not strictly true [82,83]), then the relative activity of each species is proportional to
its H-subunit composition. Hence, a shell with a composition of H4L20 would have the
capacity to oxidize iron at a rate 4-fold that of an apoferritin with only a single H-subunit. If
the fractional population of each species given by the binomial distribution is multiplied by
its relative ferroxidase activity based on H-subunit composition, the resulting distribution
then becomes normalized for differences in the population and H-subunit composition (Fig.
11B). This distribution predicts the likelihood of iron being oxidized by a particular
subpopulation of a shell within the sample, assuming no enzymatic cooperativity between H
and L subunits. Analysis of this figure shows that relatively small concentrations of an Hrich species can match or exceed the activity of another species present in somewhat higher
concentration but of lower H-subunit composition. These differences are relevant to
understanding the sedimentation patterns for HoSF shown in Fig. 4 which is composed of
15% H and 85% L subunits.
The distribution of the H-subunit within a sample (Fig. 11A) also provides an explanation
for the presence of the iron-poor fraction seen under the low flux conditions. For samples
having 15% H-chain, the fraction of material that contains one or no H-subunits accounts for
over 10% of the protein in the sample, but only ~2% of the ferroxidase activity.
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Sedimentation velocity analysis reveals the presence of an iron-poor fraction accounting for
~15% of the initial apoferritin peak under the low flux conditions (Fig. 4B). Therefore, the
iron-poor fraction observed under low-flux conditions is consistent with the anticipated
fraction of shells composed almost entirely of L-subunits. The known cooperativity between
H- and L-subunits in ferroxidase activity [84,85] would skew the distribution in Fig. 11B to
the right, resulting in a prediction of even more iron-free protein.
Evidence linking subunit heterogeneity of HoSF with iron content has been reported
previously, although prior to a full understanding of the subunit composition of HoSF [76].
This earlier study reported that the iron content of bands observed in isoelectric focusing
correlated with the surface charge of the protein in the bands. In particular, the protein in the
more acidic bands (i.e. having higher L-subunit content) had lower iron content than the
more basic bands (having higher H-content). These results are in agreement with
sedimentation results described here. It is interesting to note that the number of bands (~11)
observed using isoelectric focusing of various samples [76] corresponds favorably with the
number of species calculated from the binomial function (Fig. 11A). The relative
populations of each band correlate less well to the binomial function. However, this may be
due to the method of fractionation used in the earlier studies.
4.4. Homogeneity of iron loaded species in HuHF
NIH-PA Author Manuscript
The hypothesis that the presence of subpopulation in the core-size distributions may reflect
heterogeneity in HoSF subunit composition is further supported by the results of the
recombinant human H-chain experiments (Figs. 6 and 7). This homopolymer (H24L0)
produced rather uniform distributions under both high-flux and low-flux conditions. In both
cases, no significant shoulders or bumps in the distribution are seen. The lack of
subpopulations like those seen in HoSF is likely due to the fact that the protein is truly
homogeneous in structure (as indicated by the linewidth analysis) and activity. All the shells
are identical and will therefore respond similarly to the iron-flux condition. The lack of
fractionation of iron-poor and iron-rich species under the low-flux condition, as seen in
HoSF, add further support to this hypothesis.
NIH-PA Author Manuscript
According to Eq. (16) the mineral cores of the reconstituted HuHF ferritins in Fig. 6 have
predicted diameters ranging from 3.84 to 6.24 nm with relative standard deviations
decreasing from 8% to 3%, respectively. For a 4500 Fe core, a maximal diameter of 7.0 nm
is predicted by Eq. (16) in accord with the largest core particle size seen in TEM [63] and a
value within the ~ 8 nm internal diameter of the protein cavity. A mean core, of 6.14 nm
(range 4.11 to 8.27 nm) from TEM measurements has been reported for reconstituted HuHF
containing 1074 ±99 Fe/shell [27] which is 35% larger than the calculated diameter of 4.50
nm using Eq. (16). Measurements of the diameters of the cores of reconstituted HoSF are
also significantly larger than those predicted by Eq. (16) [28]. The differences between the
TEM measured diameters and those predicted by Eq. (16) are likely a reflection of deviation
from spherical geometry, especially for smaller nanoparticles, as evidenced by the irregular
shapes of core particles in many micrographs [63,86,87]. TEM measurements of particle
diameters also tend to be biased toward the largest dimensions of the particle. An additional
complicating factor in some TEM studies [27,28] is that only the concentration of iron and
not protein appears to have been measured following reconstitution of the protein. Because
some protein precipitation, while not visually apparent (Table 3)), always occurs during
reconstitution of ferritin, the reported Fe/shell ratios (nFe) may be significantly lower than
the true values. A low value of nFe would account for the anomalously low density of iron
predicted in the core by one study [28] and contribute to the difference between particle
diameters measured by TEM and those calculated from Eq. (16).
Biochim Biophys Acta. Author manuscript; available in PMC 2011 August 1.
May et al.
Page 18
The size and shape of nanoparticles greatly influence their properties. The fraction of the
total Fe in a spherical nanoparticle that resides on the surface is quite large due to the high
NIH-PA Author Manuscript
[88]), ranging from 0.50 to 0.24 for particles
surface to volume ratio (
containing 500 to 4500 Fe. A non-spherical particle has an even larger fraction of metal ions
on the surface. Consequently, the magnetic behavior of ferritin nanoparticles of such high
surface area is expected to be quite different from that of the bulk phase ferrihydrite,
accounting for the superparamagnetism of the mineral core and contributing to its other
interesting magnetic properties [15,22–30,88,89].
4.5. Strategies for preparing ferritin cores of uniform size
NIH-PA Author Manuscript
The results of the present study show that both native (as isolated from spleen) or
reconstituted horse spleen ferritin are fairly heterogeneous in core size and may be
unsuitable for certain studies in which the phenomena being investigated critically depend
on core particle size and the number of surface Fe3+ ions. While the incremental addition of
Fe to apoferritin results in a somewhat narrower core size distribution of the iron containing
component than when Fe is added in a single bolus (Figs. 3 and 4), substantial heterogeneity
still exists. The presence of subpopulations of core sizes appears to be an intrinsic property
of the HoSF protein, as elaborated above. HoSF is best fractionated by preparative sucrose
gradient ultracentrifugation and the fractions evaluated by analytical ultracentrifugation as
detailed here. A reasonable strategy would be to load HoSF with various amount of iron
under high-flux conditions where the mineral surface reaction [Eq. (3)] dominates and
Fenton chemistry is minimized (reducing the amount of protein precipitation) followed by
fractionation of the protein on a sucrose gradient. Dimers and trimers can be removed by
size exclusion chromatography on a Sepharose 6B column prior to sucrose gradient
fractionation [79]. Because iron is known to exchange between ferritin molecules with the
iron rich ferritins gaining iron at the expense of iron poor ferritins (reviewed in [5]), it is
prudent to stabilize samples immediately after ultracentrifugation analysis by either freezing
with a glycerol/buffer mix or lyophilization.
NIH-PA Author Manuscript
In contrast to HoSF, the human H-chain protein, being a homopolymer, produces cores of
reasonably uniform size (Figs. 6 and 7) that are suitable for direct study. However, it is
difficult to load the protein much above 2000 Fe/shell because of precipitation and,
moreover, the cores tend to be smaller and more irregular in shape compared to the L-chain
homopolymer [87]. Precipitates, while not always visible, must be removed (e.g. by
centrifugation) prior to using the sample. Cores produced with non-redox active metals that
do not utilize the H-chain ferroxidase sites are not expected to be influenced by the same
factors affecting the polydispersity of iron cores reported here where oxidation reactions
[Eqs. (1) – (3)] play major roles.
4.6. Conclusion
In conclusion, the present work has provided a detailed analysis of the sedimentation
properties of native and reconstituted ferritins. Heteropolymer ferritins exhibit an array of
core sizes in vivo or when the apoprotein is reconstituted in vitro using Fe2+ and O2. This
property is attributed to the uneven distribution of the catalytically active H-chain subunits
among the population of ferritin molecules, an idea supported by measurements with the Hchain homopolymer ferritin itself. Methods for obtaining cores of more uniform size and
assessing their homogeneity are presented.
Abbreviations and symbols
AUC
analytical ultracentrifugation
Biochim Biophys Acta. Author manuscript; available in PMC 2011 August 1.
May et al.
Page 19
NIH-PA Author Manuscript
DTT
dithiothreitol
HoSF
horse spleen ferritin
HuHF
recombinant human H-chain ferritin
MOPS
3-(N-morpholino)propanesulfonic acid
TEM
transmission electron microscopy
D
diffusion coefficient (cm2/s) under the specific conditions of protein
concentration, buffer and temperature
c
concentration (g/cm3)
diffusion coefficient of infinite dilute protein in pure water at 20 °C (cm2/s)
NIH-PA Author Manuscript
D20,w
diffusion coefficient of the protein at the stated concentration in pure water at 20
°C (cm2/s)
d
Stokes diameter (nm)
f
frictional coefficient (g/s)
g(s*)
concentration distribution function (see Fig. 2 legend)
η
viscosity (poise)
ρ
density (g/mL)
nFe
number of iron atoms per protein shell as determined by AUC or ferrozine assays
M
molar mass (g/mol)
Mr
relative molar mass (unitless)
r
Stokes radius (nm)
rm
radius of meniscus (cm)
s
Svedberg constant for the protein at stated concentration and temperature in
buffer (10−13s)
sW
weighted average of s over the g(s*) curve
Svedberg constant for infinitely dilute protein in pure water at 20°C (10−13s)
NIH-PA Author Manuscript
s20,w
Svedberg constant of protein at stated concentration in pure water at 20°C
(10−13s)
ω
angular velocity (rad/s)
σ
linewidth of Gaussian function as defined in the text (svedbergs)
ν̄
partial specific volume (mL/g)
Acknowledgments
This work was supported by NIH Grant R01 GM20194 to N.D.C and Telethon Italy Grant n. GGP05141 to P.A..
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Fig. 1.
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with iron loading of HoSF and HuHF. Curve a: Calculated line for
The variation in
HoSF using equation (9) a described in Materials and Methods. The linear-regression is
within the thickness of the line giving scomplex = 0.00882 · nFe +15.4 (R = 0.9999). Curve b:
Data for sucrose gradient fractionated HoSF ferritin from MP Biomedicals. The linear
regression through the 11 data points gives scomplex = (0.0115 ±0.0003) · nFe + 15.4
(R=0.982). Curve c: Data for monomeric HuHF reconstituted from apoHuHF with iron in 50
Fe2+ additions. The linear regression through the 5 data points gives scomplex (monomer) =
(0.0115 ± 0.0003) · nFe + 16.6 (R = 0.995). Curve d: Data for dimeric HuHF reconstituted
from apoHuHF with 50 Fe2+ additions as for curve c. The linear regression through the 5
data points gives scomplex (dimer) = (0.0181 ± 0.0005) · nFe + 23.9 (R = 0.996).
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Fig. 2.
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Sedimentation velocity profiles (g(s*) vs. s* curves) of commercially supplied holoHoSF
from Calbiochem (black —), MP Biomedicals (green – –), Sigma-Aldrich (red ˙ ˙ ˙ )
compared to laboratory prepared holo-HoSF purified from frozen horse spleen as described
in Materials and Methods (blue - ˙ - ). Sample conditions: 1.0 mg/mL ferritin in 0.1M
MOPS, 0.15 M NaCl, pH 7.5. s* is defined as
and is the transform of
the radial axis of the centrifuge tube to the sedimentation coefficient axis where ω is the
angular velocity, rj is the radius in the solution and rm is the radius of the meniscus and ti is
time. g(s*) is the concentration distribution function and is the concentration gradient on the
s* axis, i.e. g(s*) = (∂c / ∂s*)t. A detailed explanation of these quantities and their
calculation can be found in reference [90].
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Fig. 3.
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Sedimentation velocity profiles of Apo-HoSF (black —) and ferritins containing an average
of 2000 Fe/shell added either in one bolus (red ˙˙˙) or in 40 additions of 50 Fe each (green –
–). Sample conditions: 0.25 mg/mL apoferritin; 0.1 M MOPS, 0.15 M NaCl, pH 7.5. Iron
addition to the samples and correction of g(s*) for the effects of iron was performed as
described in Materials and Methods.
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Fig. 4.
Sedimentation velocity profiles of HoSF loaded either in one bolus (panel A) or in
increments of 50 Fe/shell per bolus (panel B). In each panel data are shown for Apo-HoSF
(black –) and for HoSF exposed to a total of 500 (green – –), 1000 (red ˙˙˙), 1500 (blue -˙-),
2000 (purple -˙˙-) or 2500 (magenta ---) Fe/shell. Sample conditions are as described in Fig.
3.
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Fig. 5.
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Sedimentation velocity profile of apoHuHF. Curves a, b and c correspond to the addition of
0, 1 and 5 mM DTT to the protein and demonstrate the presence of disulfide linkages. Inset:
Fitting of 5 mM DTT sample (curve a of main figure) to the sum of two Gaussian functions
with the parameters: A1 = 0.9272 ± 0.0007, speak,1 = 16.59 ± 0.002 S, σobs,1 = 2.66 ±0.01 S,
A2 = 0.1312 ± 0.0019, speak,2 = 23.9 ± 0.1 S, σobs,2 = 2.8 ± 0.1 S and y0 (offset) = 0.0519 ±
0.0008 with reduced χ2 = 0.00002 and R2 = 0.99588.
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Fig. 6.
Sedimentation velocity profiles of apoHuHF loaded with 100, 5000, 1000, 1500 and 2000
Fe/shell under low flux, the data for which form the bases for curves c and d of Fig. 1. Final
iron and protein analyses for the five samples gave 16, 667, 1908, 2621 and 3042 Fe/shell,
respectively. Sample conditions: 1 mg/mL protein in 0.1 M MOPS, 0.15 M NaCl, pH 7.5.
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Fig. 7.
Sedimentation velocity profiles of HuHF loaded either in one or two boluses (panel A) or
increments of 50 Fe/shell per bolus (panel B). In panel A data are shown for HuHF exposed
to a total of 500 in one bolus (black —), for 1000 Fe in one bolus (green – –), 1500 Fe added
in two boluses of 750 Fe/shell (red ˙˙˙), or 2000 Fe added in two boluses of 1000 Fe/shell
(blue - ˙˙ -). In panel B data are shown for HuHF exposed to a total of 500 Fe/shell in 10
additions (black —), a total of 1000 Fe/shell in 20 additions (green – –) and a total of 1500
Fe/shell in 30 additions (red ˙˙˙) of 50 Fe/shell.
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Fig. 8.
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Sucrose gradients obtained by the two layer method. Panel A: Curve a: Layering of 0 and
50% sucrose solutions resulting in a gradient from 12 to 33% sucrose; Curve b: Layering of
0 and 60% sucrose solutions resulting in a gradient from 11 to 43% sucrose; Curve c:
Layering of 0 and 70% sucrose solutions resulting in a gradient from 13 to 52% sucrose.
Inset: Standard curve of percent sucrose versus measured refractive index (n) of sucrose
solutions of known composition and containing 0.15 M NaCl. A least-squares fit of the data
yielded the following cubic equation from which the weight percent sucrose of the different
gradients was calculated: wt% = 4.3398370×l04 n3 − 1.7268340×105 n2 + 2.2975845×105 n
− 1.0222298×105. Panel B: Layering of 4.8 and 23% sucrose samples for various times. (a)
15 min; (b) 30 min; (c) 60 min and (d) overnight. Gradients obtained for curves a, b, c and d
were 6.4-22.6, 6.4-23.2, 7.5-22.7 and 12.7-17.3%, respectively. The weight percentage
sucrose (w/w) of eleven 1.5 mL fractions from top to the bottom of the centrifuge tube was
determined by refractometry.
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Fig. 9.
Sedimentation velocity profiles of individual fractions of commercial HoSF obtained on a
sucrose gradient. HoSF from M.P. Biomedicals was separated into eleven 1.5 mL fractions
on a 6.4–23% gradient. The bold line shows the sedimentation profile of the unfractionated
protein.
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Fig. 10.
Transmission electron micrographs of horse spleen ferritin samples negatively stained with
sodium silicotungstate. Upper: Micrograph of a natural ferritin sample showing both
apoferritin (A) and ferritin (F) molecules. Lower: A chemically prepared apoferritin sample
showing an apoferritin molecule (A) on the left and some ferritin molecules (F) on the right
with some of the iron core removed. The diameter of the shell of the apoferritin (A)
molecule is seen to be about 12% larger than that of the ferritin (F) molecule. Bar = 10 nm
in the upper right hand corner. Adapted and modified from reference [81] with permission.
Additional TEM images showing contraction of the protein shell can be found in reference
[63].
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Fig. 11.
The calculated binomial distributions of H-subunits for three different compositions of
HoSF, assuming no cooperativity between subunits during assembly. A. Percent of
composition. B. Relative ferroxidase activity.
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Table 1
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Sedimentation parameters for horse spleen and human H-chain ferritins.a
Protein
M (g/mol)
s20,w (10−13s)
D20,w (10−7cm2/s)
d (nm)
apoHoSF (monomer)b
484,120
17.0 (0.6)
3.21 (0.15)
13.3
apoHoSF (dimer)c
968,240
25.1 (0.1)
2.37 (0.10)
18.0
apoHuHF (monomer)d
506,266
18.3 (0.1)
3.18 (0.09)
13.5
apoHuHF (dimer)d
1,012,532
26.4 (0.2)
2.29 (0.20)
18.7
a
Estimated errors in parentheses.
Chemically prepared apoHoSF. Fractionated native HoSF (fraction # 1 of Fig. 9) gave s20,w = 16.9 S and D20,w = 3.19 × 10−7 cm2/s.
b
c
Fractionated apoHoSF (fraction # 1 of Fig. 9)
d
apoHuHF as isolated and DTT treated (curve c, Fig. 5)
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Table 2
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Comparison of the iron contents (Fe/shell ratios) of horse spleen ferritin samples determined by AUC and
ferrozine/Better Bradford analyses.
HoSF
Preparation
Catalog
Number
Fe/shella
(ferrozine/Better
Bradford)
Fe/shellb
(AUC)
Percent
Difference
CalBioChem
341476
2060
2250
9.2
MP Biomedicals
151119
2539
2480
2.3
Sigma
F4503
1990
1925
−3.2
2625
2690
2.5
Lab Prepared
a
Relative standard deviation in values is ±3.8%.
b
Estimated relative standard deviation in values is ±10%
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Table 3
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Areas under the g(s*)a curves as a function of amount of iron and conditions of exposure.
Total ironb
Low flux
%Apo-HoSFc
High flux
%Apo-HoSFc
500
0.776
91
0.926
109
1000
0.804
95
0.705
83
1500
0.675
80
0.510
60
2000
0.635
75
0.662
78
2500
0.481
57
0.734
86
a
Areas reflect the correction for the effect of added iron on the refractive increment (Materials and methods).
b
Average amount of iron added to the sample
c
Relative area of the iron-loaded samples to that seen with an identical concentration (0.25 mg/mL) of apo-HoSF, which resulted in an area of
0.851.
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