Journal of Agricultural Science; Vol. 7, No. 4; 2015
ISSN 1916-9752 E-ISSN 1916-9760
Published by Canadian Center of Science and Education
Evaluation of Growth Performance, Haematological and Serum
Biochemical Response of Broiler Chickens to Aqueous Extract of
Ginger and Garlic
Vivian U. Oleforuh-Okoleh1,2, Harriet M. Ndofor-Foleng3, Solomon O. Olorunleke1 & Joesph O. Uguru1
1
Department of Animal Science, Ebonyi State University, Abakaliki, Ebonyi State, Nigeria
2
Department of Animal Science, Rivers State University of Science and Technology, Nkpolu-Oroworukwo, Port
Harcourt, Rivers State, Nigeria
3
Department of Animal Science, University of Nigeria, Nsukka, Enugu State, Nigeria
Correspondence: Vivian U. Oleforuh-Okoleh, Department of Animal Science, Rivers State University of Science
and Technology, Nkpolu-Oroworukwo, PMB 5080, Port Harcourt, Rivers State, Nigeria. Tel: 234-803-607-2087.
E-mail: vivoleh@gmail.com
Received: January 15, 2015
doi:10.5539/jas.v7n4p167
Accepted: February 27, 2015
Online Published: March 15, 2015
URL: http://dx.doi.org/10.5539/jas.v7n4p167
Abstract
An experiment which lasted for 56 days was carried out to investigate the growth performance, haematological
and serum biochemical response of broiler chickens to aqueous extract of ginger and garlic. Eighty day-old
Marshal Strain broiler chickens were used for the experiment. The birds were randomly allotted into four
treatment groups consisting of four replicates with five birds per replicate. The aqueous extract was obtained by
infusing 14 g of each test ingredient in 1 litre of hot boiled water for 12 hours and 50 ml of the filtrate/litre of
drinking water given to birds’ ad-libitum. T1 (control), T2, T3, and T4 contained 0, and 50 mls of ginger, garlic
and a 1:1 ratio mixture of ginger and garlic in drinking water respectively. T2 gave the best performance (p <
0.05) in all growth performance traits – final body weight, weekly weight gain, weekly feed intake and feed
conversion ratio and T1 the least. Significant (p < 0.01) increases were observed in haemoglobin concentration,
packed cell volume, white blood cell, and red blood cell of the ginger and garlic treated birds. The serum
biochemical parameters measured were significantly (p < 0.05) different, with T2 and T3 showing a better
response. Cholesterol decreased significantly (p > 0.05) while there was significant increase in the total protein,
albumin, and globulin of the treated birds (p < 0.01). Administration of ginger and garlic to broiler chickens
increased their performance, boosted their immunity as well as improved their general well-being. It is, thus,
recommended in broiler chicken production.
Keywords: aqueous extract, body weight, chicken, cholesterol, feed intake, haematology, total protein
1. Introduction
In Nigeria, the effect of inadequate animal protein intake is felt more by a large proportion of the population
especially in the rural areas. Poultry meat is a good source of animal protein and can contribute immensely in
boosting the consumption level of animal protein. The prohibitive increase in the cost of input especially that of
feed is among the constraints in commercial broiler production (Madubuike & Ekenyem, 2001). Ensuring more
net return and minimizing high expenditure for feed are the main challenges, for which many research strategies
have been trying to address through the inclusion of feed supplements and feed additives in the diets of broiler
chicken.
A major feed additive that has been extensively used is antibiotics. Antibiotics use in livestock is the use of
antibiotics for any purpose in the husbandry of livestock, which include not only the treatment or prophylaxis of
infection but also the use of sub-therapeutic doses in animal feed to promote growth and improve feed efficiency
in contemporary intensive animal farming (Ogle, 2013). Incidentally, their use in animal feed has shown several
side effects such as resistance towards the drug and evidence of resistant strains that become zoonotic (Wegener
et al., 1999). The emergence of antibiotic resistance by pathogenic bacteria has led to international restriction on
the use of antibiotics in animal feeds. Consequently, the poultry industry is under great pressure to minimize
their use in animal feed and seek alternatives. These alternatives can be found in the use of herbs and spices
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materials as supplements. According to Manesh et al. (2012) natural alternatives to antibiotics, such as herbs and
medicinal plants, have attracted attention due to their wide range of potential beneficial effects.
Natural medicinal products originating from herbs and spices have been used as feed additives for farm animals
(Guo, 2003). The efficacy and importance of a particular feedstuff/feed ingredient in poultry production is
evaluated from its effect on the production performance/traits of the birds. Furthermore, valuable information
can be obtained from the study of the haematological parameters. This stems from the fact that the blood serves
as an important index of physiological, pathological and nutritional status of an animal. Information obtained
from haematological assay, apart from being useful for diagnostic and management purposes could equally be
incorporated into breeding programmes (Elagib & Ahmed, 2011). Two herbal plants which are nutritionally
adequate and locally available in Nigeria that can be harnessed as feed additives are ginger (Ademola et al., 2009)
and garlic (Gbenga et al., 2009). Ginger is a rhizomatous herbaceous plant, whose rhizome is used medicinally.
Ginger contains several compounds and enzymes including gingerdiol, gingerol, gingerdione and shogaols
(Rivlin, 2001; Zhao et al., 2011). These compounds have been reported to have antimicrobial, antioxidative and
pharmacological effects (Al-Amin et al., 2006; Tapsell et al., 2006; Ali et al, 2008). Garlic is best known as a
spice and herbal medicine for treatment and prevention of an array of diseases (Adibmoradi et al., 2006). The
key active ingredient in garlic is a powerful plant chemical called allicin which rapidly decomposes to several
organosulphur compounds with bioactivities (Chang & Cheong, 2008). Several reports are available advocating
the roles of these herbs in improving growth performance, meat quality, anti-cholesteremic effects and as well as
immuno-modulating effects on broiler chickens (Gardzielewska et al., 2003; Aji et al., 2011; Ashayerizadeh et al.,
2009; Hanieh et al., 2010; Ayasan, 2011).
The present study was carried out to evaluate the growth performance, haematological and serum biochemical
indices of broiler chicks fed ginger and garlic aqueous extract.
2. Materials and Methods
2.1 Study Location
The present study was carried out at the Poultry Unit of the Teaching, and Research Farm of the Department of
Animal Science, Ebonyi State University Abakaliki. Abakaliki is located within the south eastern guinea
savannah ecological zone between latitude 8o30′ and 9o40′ North and longitude 5o40′ and 6o45′ East (Nwakpu,
2005). The experiment lasted for 49 days.
2.2 Test Ingredients
The fresh ginger and garlic used were purchased from Abakaliki market. The garlic and ginger were peeled, cut
into chips and sundried for a period of six weeks. The dried garlic and ginger chips were ground into smooth
powder and stored separately in an air tight container. Each aqueous extract was prepared by adding one liter of
boiled hot water to 14 g of either ground ginger or garlic or a mixture of the two (at 7 g each) in separate
non-metallic containers. The mixtures were allowed to infuse and cool at room temperature overnight for twelve
hours. The next morning, the extract was obtained by filtering the infusion using a filter paper, and then
administered to the chicks in their drinking water at 50 ml/liter of water. The aqueous extract was made available
to the birds for ad libitum. Fresh infusion was prepared daily. The procedure used for preparing the aqueous
extract is in line with Leila (1977).
2.3 Experimental Animals and Treatments
A total of eighty day-old Marshal broiler chicks used for the study were purchased from Obasanjo Farms Nigeria
Limited, Ota in Ogun State. The chicks were kept for seven days to acclimatize; within this period, they were fed
commercial broiler starter diet only and given plain drinking water. On the 8th day, the 80 chicks, having an
average body weight of 121.66 ± 5.00 g were randomly allotted to four experimental treatments in a completely
randomly design (CRD). Each treatment was replicated four times with 5 chicks per replicate. The birds were
housed in a wooden three-tier battery cage, each replicate cell measuring 30 × 44 × 21 inches.
Four experimental treatments identified as T1, T2, T3, and T4 were studied. Birds on T1 (control treatment)
received basal diet (commercial starter diet -22% CP, 2900 kcal/kg ME- fed for the first 28 days and finisher diet
fed from the 28 to 49 day (18% CP, 2900 kca/kg ME), and water without ginger or garlic. Those on T2 received
the basal diet and were given ginger extract at 50 ml/litre of water; birds on T3 received the basal diet and were
given garlic extract at 50 ml/litre of water, and those T4 were fed the basal diet and received extract of both
ginger and garlic at 25 ml each/litre of water. Proper management, necessary vaccinations and good
environmental condition were maintained throughout the period of study.
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2.4 Data Collection
2.4.1 Growth Performance
Data were collected on growth performance traits (such as daily feed intake, weekly body weight, final body
weight and feed conversion ratio).
2.4.2 Haematological and Serum Biochemical Assay
On the 56th day of study, blood samples were randomly collected from four birds/treatment. The blood samples
were collected via the wing veins using sterile needles and syringes. The blood samples for haematological
parameters were collected into well-labeled and sterilized bottles containing ethylene diamine tetra acetic acid
(EDTA), as anti-coagulant. The samples were investigated for the following haematological parameters – packed
cell volume (PCV), red blood cell count (RBC), white blood cell (WBC), haemoglobin and platelets (Lamb,
1991). Blood samples for biochemical indices were collected into another sample bottles without the
anticoagulant. Plasma samples were analyzed for cholesterol, total protein, albumin, globulin and urea. The
serum biochemical indices were done using the clinical routine procedures outlined by Olorede et al. (1996)
2.5 Statistics and Data analysis
The data collected were statistically analyzed by analysis of variance using Repeated Measures in General Linear
Model in the statistical package SPSS (2009) using the following statistical model:
Xij = µ + Hi + Εij
(1)
Where,
Xij = any observation made in the experiment; µ = the population mean; Hi = effect of type of herb (I = ginger,
garlic); Εij = residual error.
3. Results and Discussion
3.1 Growth Performance
The summary of the effect of the different herbs on growth performance traits of broiler chickens is presented in
Table 1. There were significant differences (P < 0.05) between treatments in performance traits. Birds on ginger
infusion had a better performance for all traits studied, there was a 29.07, 16.60 and 14.98% significant (P < 0.05)
increase in final body weight relative to T1, T3 and T4 respectively. The present findings affirm the work of
Herawati (2010) on Hubbard broiler strains. The authors observed a significant increase in final body weight,
higher feed intake and better feed conversion ratio of birds fed 2% supplemented red ginger in their diet). Similar
result was obtained by Al-Moramadhi (2010) when broiler chicks were given ginger orally at 100 mg/kg body
weight for six weeks. Minh et al. (2010) and Onu (2010) also reported that supplementation of dried ginger to
broiler diets resulted in improved performance. Ademola et al. (2009) also observed that ginger increased body
weight when included in the diet up to 2% level in the diet. The better performance observed in T2 could be
attributed to some medicinal properties contained in ginger. For instance, a protein digesting enzyme (zingibain)
found in ginger is believed to improve digestion as well as kill parasites and their eggs. Furthermore, properties
in ginger tends to enhance antibacterial and anti inflammatory factors (Mohammed & Yusuf, 2011). Tekeli (2007)
stated that due to the active ingredients in these herbs, there is the formation of more stable intestinal flora and
improved feed conversion efficiency in consequence of a better digestion.
Table 1. Growth Performance of broiler chicks given aqueous infusion of ginger and garlic
Parameters
T1
0 ml
T2
50 ml ginger
T3
50 ml galic
T4
50 ml ginger & garlic
SEM
P-Value
Final body weight (g)
1930.80c
2684.40a
2238.20b
2282.20b
Weekly weight gain(g)
Weekly feed intake(g)
Feed Conversion Ratio
c
274.99
b
538.73
a
1.96
383.49
a
567.08
a
1.48
c
319.74
b
535.30
b
1.67
b
24.07
0.00
326.03
b
3.41
0.00
534.48
b
4.19
0.00
0.125
0.00
1.64
b
abc
Note. Means on the same row followed by different superscripts are significantly different (p < 0.05); SEM –
Standard Error of Mean.
Though in the present study, birds on T2 performed better than those on T3 and T4, these two treatments were
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significantly (p < 0.05) better than the control. Mahmood et al. (2009) reported that garlic had positive effect on
the growth rate of broiler chicks. Meraj (1998) noted that the presence of antibiotic substances in garlic is
responsible for the improvement of weight gain. In consonance, Rehman et al. (2012) reported that mean feed
conversion ratio was significantly influenced by water based infusion of garlic and Withania somnifera. The
reports of Ashayerizadeh et al. (2009) and Mohebbifar and Torki (2011) showed that inclusion of garlic powder
in broiler feed did not change body weight, feed intake and efficiency/feed conversion ratio. Lawson et al. (1992)
observed that allicin is unstable and poorly absorbed from the digestive tract. Present findings also support the
findings of Javed et al. (2009) which showed a positive effect of aqueous extract of plant mixture (Zingiber
officinale, Carum apticum, Withania somnifera, Trigonella Foenum-Graecum, Silybum marianum, Allium
sativum and Berberis lyceum) on the performance of broiler chicks in term of weight gain and Feed Conversion
Ratio.
3.2 Haematological and Serum Biochemical Assay
The results of the haematological and serum biochemical response of broiler chickens administered aqueous
extract of test ingredients are presented in Table 2.
Table 2. Haematological and serum biochemical response of broiler chickens administered aqueous extract of
test ingredients
Parameters
T1
0 ml
T2
50 ml ginger
T3
50 ml galic
T4
50 ml ginger & garlic
SEM
P-Value
PCV (%)
24.89b
28.22ab
29.14a
25.5b
1.96
0.010
8.38
b
0.38
0.001
2.46
b
0.16
0.001
6.80
b
0.22
0.001
15.42
0.173
8.00
b
2.15
c
WBC(10 /mm )
6.30
c
Platelets
166.00
HB(g/dl)
12
3
RBC (10 /mm )
9
3
Cholesterol(mg/dl)
Protein(g/l)
Albumin (g/l)
245.43
4.50
c
2.56
c
Globulin(mg/dl)
1.78
Urea(mg/dl)
42.00
2.62
ab
7.10
ab
140.00
a
c
9.30
a
225.77
5.85
b
3.44
b
2.40
a
40.00
9.73
a
2.80
a
7.49
a
150.00
a
200.00
6.54
a
4.02
a
2.53
a
40.36
b
170.00
230.37
a
14.59
0.048
5.38
b
0.29
0.000
3.32
b
0.27
0.000
2.04
b
0.18
0.004
41.10
1.67
0.503
abc
Note. Means on the same row followed by different superscripts are significantly different (p < 0.05); SEM –
Standard Error of Mean.
There was significant increase (P < 0.05) in the PCV, Hb, RBC, and WBC of birds on the ginger and garlic
infusion than those on control treatment. Mitruka et al. (1977) stated that the number of erythrocytes (RBC) in
chicken is influenced by the conditions of the animal. The increase in PCV, Hb, and RBC contents of the blood
of birds fed the test ingredients is an indication of improved oxygen carrying capacity of the cells which
translated to a better availability of nutrients to the birds consequently affecting their well-being. Sole
administration of ginger and garlic numerical reduced the platelets in the blood. Muhammed and Lakshmi (2007)
opined that inhibiting the transformation of arachidonic acid to thromboxane and decreasing the sensitivity of
platelets to aggregating agents may be possible with the administration of ginger in fatty diets. This implies that
ginger could be potentially useful in improving blood circulation on account of its inhibitory effects on platelet
aggregation (Muhammed & Lakshmi, 2007). Similar inhibitory effect was observed in garlic by Lawson et al.
(1992).
The result on serum biochemical indices indicates that inclusion of ginger and garlic in the water of broiler
chickens successfully reduced the cholesterol in the serum. The present findings also reveal that the ginger
extract caused reduction in the levels of serum cholesterol, though the mixture of the two (ginger and garlic) did
not show any variation from the control where the cholesterol statistically increased. Saeid et al. (2010) observed
that aqeous extract of ginger significantly reduced the level of cholesterol in the blood of broilers. Bhandari et al.
(1998) and Akhani et al. (2004) also reported that ginger treatment significantly decreased serum cholesterol.
This affirms the findings of Mansoub (2011) who reported reductions in total cholesterol when broilers were
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supplemented with 1 g/kg garlic. The results of present study is, also, in agreement with Stanacev et al. (2011)
who reported that garlic manifested hypocholesterolemic effects on chickens through inhibition of the most
important enzymes that participate in the synthesis of cholesterol and lipids. Konjufca et al. (1997) reported that
garlic reduced plasma cholesterol by decreasing the activity of 3-hydroxy-3-methlyglutaryl reductase. Allicin has
been proposed as the active compound in garlic responsible for health promotion and hypocholesterolaemic
benefits (Lawson, 1998). The result of the present study affirms the findings of Al-Moramadhi (2010) on the
effect of ginger root infusion on haematological parameters in broiler chickens. The reduction in cholesterol
level can be traced to the presence of gingerols and shagols components in ginger which inhibits lipid
peroxidation (Verma et al., 2004; Ashani & Verma, 2009).
4. Conclusion
A mixture of the two herbs was not as beneficial as sole treatment (treatment either with ginger or garlic) in all
parameters studied. Although, birds on aqueous extract of ginger, had better performance in terms of final body
weight and feed conversion ratio, administration of aqueous extract of garlic in the broilers drinking water
improved the haematological and serum parameters studied. The result of the present study, therefore, suggests
that the use of ginger and garlic as aqueous extract in the diets of broiler chickens improved their performance as
well as their health status. Generally, administration of aqueous extract of ginger and garlic is recommended in
broiler production for improved nutritional and physiological traits in broiler chickens.
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