Microbial genetics and cell biology
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Recent papers in Microbial genetics and cell biology
In the present study, biosorption of Chromium by microorganisms from Coal mine effluent was investigated. The initial concentration of heavy metal Chromium (K2Cr2O7) in the effluent was 120mg/lit. The bacteria isolated from industrial... more
Adsorption of colicin B to a sensitive strain of Escherichia coli results in rapid cessation of deoxyribonucleic acid, ribonucleic acid, and protein synthesis. Some classes of mutants insensitive to colicin B hyperexcrete a colicin... more
This chapter reviews my 18 years of research in Anne's Unit including studies on temporal and spatial aspects of X-chromosome inactivation and imprinting and the role of methylation in X-inactivation in these processes during female... more
Type II CRISPR-Cas systems defend prokaryotes from bacteriophage infection through the acquisition of short viral DNA sequences known as spacers, which are transcribed into short RNA guides to specify the targets of the Cas9 nuclease. To... more
A general system has been devised for the in vivo construction of Candida albicans integrative vectors in Saccharomyces cerevisiae. The system is especially useful for the integration of genes in C. albicans that cannot be propagated in... more
Many bacteria and archaea have the unique ability to heritably alter their genomes by incorporating small fragments of foreign DNA, called spacers, into CRISPR loci. Once transcribed and processed into individual CRISPR RNAs, spacer... more
Some R factors, like some colicin factors, confer partial protection against the bactericidal effect of ultraviolet (UV) irradiation. Of 31 plasmids (17 R, 3 col, and 11 R-col factors) tested in Escherichia coli K-12, 15 protected, 11 had... more
Purified preparations of Rous sarcoma virus (RSV) contain ribonuclease which is either a constituent of the virion surface or an adsorbed contaminant. Treatment of the virus with nonionic detergent to activate ribonucleic acid... more
ABSTRACTHere, we suggest that natural streptomycin resistance of many sphingomonads resides withinrpsL. We constructed a dominant, streptomycin-sensitiverpsLallele and demonstrated its use as a counterselection marker in several... more
Yeast is a powerful model for systems genetics. We present a versatile, time- and labor-efficient method to functionally explore the Saccharomyces cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput... more
Strains from various staphylococcal species produce bacteriocin peptides, which are thought to play important roles in bacterial competition and offer interesting biotechnological avenues. Many bacteriocins are secreted as inactive... more
Burkholderia cenocepacia is an emerging opportunistic pathogen causing life-threatening infections in immunocompromised individuals and in patients with cystic fibrosis, which are often difficult, if not impossible, to treat.... more
The infection of Ltk minus cells with Herpes Simplex virus (HSV) induces a TK activity with an average R f of 0.43. HSV transformed cells, ie, clones of Ltk minus cells which have acquired the ability to take up dThd from tissue culture... more
It is now widely accepted that the application of standard microbiological methods for the recovery of microorganisms from the environment has had limited success in providing access to the true extent of microbial biodiversity. It... more
Bardet-Biedl Syndrome (BBS) is a genetically and clinically heterogeneous disorder clinically characterized by obesity, mental retardation, dysphormic extremities (syndactyly, brachydactyly or polydactyly), retinal dystrophy or pigmentary... more
Maintenance of long-term cultures of yeast cells is central to a broad range of investigations, from metabolic studies to laboratory evolution assays. However, repeated dilutions of batch cultures lead to variations in medium composition,... more
Acute renal failure (ARF) is a syndrome associated with high mortality rate in humans. ARF now known as acute kidney injury (AKI) represents a significant and devastating problem in clinical medicine. This work was performed on adult male... more
We developed a counter-selectable deletion system for Thermus thermophilus HB27 based on cytosine deaminase (codA) from Thermaerobacter marianensis DSM 12885 and the sensitivity of T. thermophilus HB27 against the antimetabolite... more
Metagenomics (also referred to as environmental and community genomics) is the genomic analysis of microorganisms by direct extraction and cloning of DNA from an assemblage of microorganisms. The development of metagenomics stemmed from... more
It is now widely accepted that the application of standard microbiological methods for the recovery of microorganisms from the environment has had limited success in providing access to the true extent of microbial biodiversity. It... more
The general biological properties of a temperature-sensitive morphological mutant of Bacillus subtilis (168ts-200B) are described. At the restrictive temperature (45 C), cells grow as spheres which divide irregularly to form grapelike... more
Exposure of Escherichia coli to critical acridine orange (AO) concentrations did not result in loss of viability. However, the deoxyribonucleic acid (DNA) of cells exposed to such agents was rapidly degraded and repolymerized. On the... more
Polyhydroxyalkanoates (PHA) synthesis genes phbC and orfZ cloned from Ralstonia eutropha H16 were transformed into beta-oxidation weakened Pseudomonas putida KTOY08ΔGC, a mutant of P. putida KT2442. The recombinant P. putida strain termed... more