Size Exclusion Chromatography

The purpose of the study was to optimise analytical methods for determination of the chemical speciation of mercury in studies of protective mechanisms of selenium. Optimisation of the methods was performed using CRM DOLT-2 (Dogfish liver), both in its original form and after separation of various fractions. The sample was homogenised with 10 mM Tris-HCl buffer (pH 7.6) and ultracentrifuged. The soluble phase obtained was applied to a size exclusion chromatography column (Sephadex G-75 column) for separation of various protein fractions. Total mercury (total Hg), monomethyl mercury (MeHg) and selenium (Se) were determined in whole dogfish liver tissue and its soluble and insoluble phases (pellet). Different approaches for determination of total Hg and MeHg were compared. Simultaneous determination of MeHg and inorganic mercury (Hg2+) was based on alkaline dissolution and/or acid leaching, followed by ethylation, room temperature precollection, isothermal gas chromatography (GC), pyr...

A combination of the complex (1-Me-indenyl)(PPh3)NiCCPh and methylaluminoxane (MAO) catalyzes the polymerization of 1-ethynylpyrene to fairly soluble cis-poly(1-ethynylpyrene). The polymers have been characterized by IR, solid state 13C and 1H NMR, and UV-Vis (λmax at 346nm) spectroscopy, and studied by thermogravimetric analysis (T10∼285–392°C), differential scanning calorimetry, and size exclusion chromatography (Mw∼103–104Da; Mw/Mn∼2). The thermal and optical properties of these cis-polymers

The purpose of the study was to optimise analytical methods for determination of the chemical speciation of mercury in studies of protective mechanisms of selenium. Optimisation of the methods was performed using CRM DOLT-2 (Dogfish liver), both in its original form and after separation of various fractions. The sample was homogenised with 10 mM Tris-HCl buffer (pH 7.6) and ultracentrifuged. The soluble phase obtained was applied to a size exclusion chromatography column (Sephadex G-75 column) for separation of various protein fractions. Total mercury (total Hg), monomethyl mercury (MeHg) and selenium (Se) were determined in whole dogfish liver tissue and its soluble and insoluble phases (pellet). Different approaches for determination of total Hg and MeHg were compared. Simultaneous determination of MeHg and inorganic mercury (Hg2+) was based on alkaline dissolution and/or acid leaching, followed by ethylation, room temperature precollection, isothermal gas chromatography (GC), pyr...

Bioerodible polymers used in controlled drug delivery systems, such as poly(lactic-co-glycolic acid) (PLGA) undergo radiolytic degradation during γ-irradiation. In spite of the considerable practical importance, yet only little knowledge is available on the consequences of this sterilization method on the resulting drug release patterns in a quantitative way. The major objectives of the present study were: (i) to monitor the

To investigate the composition of dissolved organic matter (DOM) as a function of apparent molecular weight (MW) by rapid analytical methods, high performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) was conducted with sequential on-line detectors ...

Gel filtration chromatography using a TSKgel G2000 SW column coupled with differential refractive index detection was used to analyse five grades of polyvinyl alcohol. Limits of detection and quantification for the assay were 0.14 mg mL -1, 0.47 mg mL -1 respectively. The inter and intra-day co-efficient of variance were both pn=5) between the calibration curves across the five grades

Bread represents a suitable food product for the addition of functional ingredients, such as the cholesterol-lowering dietary fibre oat β-glucan and the prebiotic inulin. Therefore, these soluble fibres were incorporated into wheat as well as gluten-free bread, and their effects on rheological properties of the dough, on bread quality and on crumb microstructure were compared. The level of remaining β-glucan as well as its molecular weight was determined using an enzyme kit and size-exclusion chromatography. The addition of oat β-glucan resulted in a higher water addition level, whereas incorporation of inulin had the opposite effect. Rheological testing showed that the incorporation of oat β-glucan results in a more elastic dough. The baking characteristics mainly affected by fibre addition were volume and crust colour, with inulin increasing and oat β-glucan decreasing loaf-specific volume in the gluten-free breads. Inulin led to a darkening of the crust of both bread types, whereas addition of oat β-glucan resulted in a lighter crust of gluten-free bread. Oat β-glucan softened the crumb of gluten-free bread, but had the opposite effect on wheat bread. Inulin resulted in an increased crumb hardness as well as the rate of staling. Beta-glucan breakdown was more pronounced in wheat bread than in gluten-free bread. The results show that the use of β-glucan to increase the nutritional value of wheat bread is limited due to negative influences on technological properties. However, this soluble fibre is highly suitable for incorporation into gluten-free bread.

Corn starches of different amylose contents (waxy, normal, and high amylose) were dissolved in a strong alkaline solution (1 M NaOH) by vigorous vortexing at room temperature, and analyzed by a medium-pressure size exclusion chromatography (SEC), using 50 mM NaOH as eluent, connected to refractive index and multi-angle laser light scattering detectors. The vortexing increased the solubility of the corn