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    Pex 08 04

    Uploaded by

    Linette Guillermo
    This document summarizes the results of a student's lab report on assessing lipase digestion of fat. The student scored 75% on the pre-lab quiz by correctly answering questions about bile, the substrate used, and the products of lipid digestion. In the experiment, the student predicted the tube with the lowest pH would have highest lipase activity but the results showed the highest activity in the tube with bile salts and an initial pH of 7. The student's review answers explained why fully testing lipase in the lowest pH tube was difficult and that bile emulsifies lipids to improve lipase activity.

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    © All Rights Reserved
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    Pex 08 04

    Uploaded by

    Linette Guillermo
    16K views4 pages
    This document summarizes the results of a student's lab report on assessing lipase digestion of fat. The student scored 75% on the pre-lab quiz by correctly answering questions about bile, the substrate used, and the products of lipid digestion. In the experiment, the student predicted the tube with the lowest pH would have highest lipase activity but the results showed the highest activity in the tube with bile salts and an initial pH of 7. The student's review answers explained why fully testing lipase in the lowest pH tube was difficult and that bile emulsifies lipids to improve lipase activity.

    Original Title

    PEX-08-04

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    This document summarizes the results of a student's lab report on assessing lipase digestion of fat. The student scored 75% on the pre-lab quiz by correctly answering questions about bile, the substrate used, and the products of lipid digestion. In the experiment, the student predicted the tube with the lowest pH would have highest lipase activity but the results showed the highest activity in the tube with bile salts and an initial pH of 7. The student's review answers explained why fully testing lipase in the lowest pH tube was difficult and that bile emulsifies lipids to improve lipase activity.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    16K views4 pages

    Pex 08 04

    Uploaded by

    Linette Guillermo
    This document summarizes the results of a student's lab report on assessing lipase digestion of fat. The student scored 75% on the pre-lab quiz by correctly answering questions about bile, the substrate used, and the products of lipid digestion. In the experiment, the student predicted the tube with the lowest pH would have highest lipase activity but the results showed the highest activity in the tube with bile salts and an initial pH of 7. The student's review answers explained why fully testing lipase in the lowest pH tube was difficult and that bile emulsifies lipids to improve lipase activity.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report

    Pre-lab Quiz Results


    You scored 75% by answering 3 out of 4 questions correctly.
    1. Which of the following is/are true of bile?
    Your answer : d. It works by a chemical and physical process.
    Correct answer: b. It works by a physical process.
    2. The substrate used in this simulation is
    You correctly answered: c. vegetable oil.
    3. When fatty acids are liberated by lipase, the pH
    You correctly answered: b. decreases.
    4. One of the products of the chemical digestion of lipids is
    You correctly answered: d. fatty acids.

    04/13/16

    page 1

    Experiment Results
    Predict Question:
    Predict Question: Which tube do you think will have the highest lipase activity?
    Your answer : d. tube 5
    Stop & Think Questions:
    What does the pH measure?
    You correctly answered: d. both lipase activity and fatty acid release
    Why is it difficult to detect whether lipase is active in tube 5?
    You correctly answered: d. The pH is already very low, so a decrease in pH might be difficult to detect.
    Experiment Data:
    Tube No.
    1
    2
    3
    4
    5
    6

    Reagent 1
    Lipase
    Lipase
    Lipase
    Water
    Lipase
    Lipase

    Reagent 2
    Vegetable oil
    Vegetable oil
    Water
    Vegetable oil
    Vegetable oil
    Vegetable oil

    Reagent 3
    Bile salts
    Water
    Bile salts
    Bile salts
    Bile salts
    Bile salts

    04/13/16

    Reagent 4
    pH 7.0
    pH 7.0
    pH 9.0
    pH 7.0
    pH 2.0
    pH 9.0

    page 2

    Time
    60
    60
    60
    60
    60
    60

    Temp.
    37
    37
    37
    37
    37
    37

    pH
    6.21
    6.72
    9.00
    7.00
    2.00
    8.97

    Post-lab Quiz Results


    You have not completed the Post-lab Quiz.

    04/13/16

    page 3

    Review Sheet Results


    1. Explain why you can't fully test the lipase activity in tube 5.
    Your answer:
    Lipase activity in tube 5 is difficult to test because the reagent put into the tube is already very acidic (pH 2.0). That is why
    after treatment and incubation, the resulting pH is still very acidic. It is difficult to determine if this is due to lipase activity or if
    it is simply because of the acidic reagent.
    2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible
    reasons why it may or may not have matched.
    Your answer:
    My prediction and the results did not match. Highest lipase activity actually occured in the first tube wherein pH lowered the
    most.
    3. Explain why pancreatic lipase would be active in both the mouth and the pancreas.
    Your answer:
    Digestion is already happening in the mouth, which explains why lipase is active in the mouth. The same is true for the
    pancreas.
    4. Describe the process of bile emulsification of lipids and how it improves lipase activity.
    Your answer:
    Emulsification separates clumps of bile. This aids lipase activity.

    04/13/16

    page 4

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