BIOPROCESS TECHNOLOGY

Course Code: BTB 701 Credit Units: 03

Module I
Advantage of bioprocess over chemical process. Basic principle in bioprocess technology. Media formulation, Cell
culture techniques; Inoculum development and aseptic transfers. Different types of pumps, valves, and line
materials, piping conventions etc. used in Biochemical Process
Module II
Process technology for the production of primary metabolites, eg. biomass, ethanol, acetone-butanol, citric acid,
amino acids, polysaccharides and plastics.
Ethanol: production by batch, continuous and cell recycle adopted by various technologies practiced in Indian
distilleries using molasses and grains. Computation of fermentation efficiency, distillation efficiency and overall
efficiency of ethanol production, recovery, uses, glucose effect etc. Power alcohol – definition, uses, merits and
demerits of various technologies for its production.
Amino Acid: Genetic Control of metabolic pathway.
Lysine: Indirect and direct fermentation – mechanism of ph of metabolic block in accumulation of L- lysine by
inhibition and repression mechanism.
Biomass: Bakers and distillers yeast production using various raw materials, “bios” factors for growth, Crabtree
effect, harvesting, different forms and uses.
What are mushroom, different forms of common mushroom production from agro based raw materials and uses.
Biofertilizers, biocompost and biopesticides
Module III
Production of secondary metabolites – penicillin, cephalosporins, streptomycin, tetracycline etc. Metabolites from
plant and animal cell culture
Penicillin: Classification, various penicillin as precursor and ‘R’ – side chain, penicillinase, 6-APA, penicillin
production, harvest and recovery, uses of various forms etc.
Streptomycin: chemical structure, production, harvest and recovery, use by-product of streptomycin fermentation
etc.
Tetracycline: chemical structure, production, harvest and recovery, use by-product of tetracycline fermentation etc.
Module IV
Microbial production of industrial enzymes – glucose isomerase, penicillin acylase, cellulase, amylase, lipase,
protease etc.

[ almost ol notes been given,those left of 1st mod r not availabl 2 me nlyy…will try nd get Bando
sir’s notes on 18th]

DOWNSTREAM PROCESSING
Course Code: BTB 702 Credit Units: 03
Module I
Characteristics of Bioproducts; Coagulation, Flocculation and conditioning of broth.
Module II
Mechanical separation; Cell disruption techniques
Module III
Protein precipitation and separation
Module IV
Aqueous- two- phase extraction, Adsorption-desorption processes
Module V
Chromatographic methods of separation based on size, charge, hydrophobic interactions and biological affinity
Module VI
Membrane based separation; Dialysis, Electrodialysis; Micro filtration, Ultra filtration; Electrophoresis
Module VII
Crystallization; Drying

[ol remaining topics jinke notes nahii diyee wo saare sivasankar se padhne hai]
 PRINCIPLES OF MANAGEMENT AND ENTREPRENEURSHIP
DEVELOPMENT
Course Code: BTB 703 Credit Units: 03
Module I
Principles and function of management, Planning and decision making, Line and staff relationship, management by
objective
Module II
Formal and informal organization, Performance appraisal, Training and development
Module III
Entrepreneurship and entrepreneurial process, Business plan, Form of ownership suitable for business
Module IV
Entrepreneurial motivation and leadership, entrepreneurial competencies, entrepreneurial development programme
[ ol soft copies are on googlegroup and a handout of 12 page is ther…that u’l get on mon]

[In GnP ol those topics left must be read from net or any book….]
PHARMACEUTICAL CHEMISTRY AND DRUG DESIGN
Course Code: BTB 705 Credit Units: 03
Module I
Introduction of pharmaceutical Chemistry, Overview of drug discovery process.
Physicochemical Properties in Relation to Biological Action – Effects of route of administration, sites of loss,
Solubilities and partition coefficients (Ferguson, Hansch), Drug-receptor interactions, Steric features of drugs, The
drug receptor, structure-Activity Relationships, Representatives physicochemical properties as relation to biological
action
Module II: Drug Targets and their validation
Drug targets classification-DNA, RNA, post-translational, processing enzymes, metabolic enzymes involved in
nucleic acid synthesis, G-protein coupled receptors (monomeric transmembrane proteins), small molecule receptors,
neuropeptide receptors, ion channels (monomeric multi-transmembrane) proteins, ligand-gated ion channels
(oligomeric transmembrane proteins), transporters (multi-transmembrane proteins
Validation Strategies
Module III: Drug Design Strategies
A. Structure-based design-Docking and denovo methods
B.Design and development of combinatorial libraries for new lead generation
The molecular diversity problem, drug characterization – principles of equilibria, diffusion and kinetics,
preformulation: pKa, partition coefficient, solubility, dissolution, chemical stability, and permeability, optimization
of ADME characteristics, physico-chemical properties calculation, chemiometrics in drug design.
C. QSAR
Statistical techniques behind QSAR, classical QSAR, molecular descriptors 3D QSAR and COMFA, drug design to
discovery and development, drug metabolism, toxicity and pharmacokinetics, toxicology considerations, problems
and drawbacks on drug discovery and development.
Module IV
Drug toxicity, tolerance, dependence, addiction, Dose Response curves
Module V
Survey of various Drug Classes – Anaesthetics (general, local), Analgescis, Neurotransmitters (adrenergic,
cholinergic effects; psychopharmacology), CNS depressants (sedative/hypnotic, major/minor tranquilizers), CNS,
Stimulants, Antibiotics (especially b-lactam), Steroids- Mechanism of action and applications
 ENVIRONMENTAL BIOTECHNOLOGY
Course Code: BTB 707 Credit Units: 03
Module I: Introduction
Ecology and ecosystem.
Module II: Environmental pollution
Water, soil and air, noise and thermal pollution, their sources and effects.
Module III: Waste water (sewage and industrial effluents) treatments
Anaerobic and aerobic treatment, conventional and advanced treatment technology, methanogenesis,
methanogenic, acetogenic, and fermentative bacteria- technical process and conditions, emerging biotechnological
processes in waste - water treatment.
Module IV: Solid waste management
Landfills, composting, earthworm treatment, recycling and processing of organic residues.
Module V: Biodegradation
Biodegradation of xenobiotic compounds, organisms involved in degradation of chlorinated hydrocarbons,
substituted simple aromatic compounds, polyaromatic hydrocarbons, pesticides, surfactants and microbial treatment
of oil pollution
Module VI: Microbial leaching and mining
Microbial leaching and mining: Extraction of metals from ores; Recovery of metals from solutions; Microbes in
petroleum extraction; Microbial desulfurization of coal.
Module VII: Wasteland
Wasteland: Uses and management, bioremediation and biorestoration of contaminated lands.
Module VIII: Environmental genetics
Environmental genetics: degradative plasmids, release of genetically engineered microbes in environment.
Module IX: Hazardous wastes
Hazardous wastes: source management and safety.

BIOPROCESS TECHNOLOGY LAB

Course Code: BTB 720 Credit Units: 01
Module I
Isolation of industrially important micro organisms for microbial processes.
Module II
Determination of Thermal Death Point and Thermal death time of micro organisms for design of a sterilizer
Module III
Determination of growth curve of a supplied micro organism and also determine substrate degradation profile and to
compute specific growth rate and growth yield from the data obtained.
Module IV
Comparative studies of ethanol production using different substrates.
Module V
Production of single cell protein
Module VI
Production and estimation of alkaline protease
Module VII
Sauer Krant fermentation
Module VIII
Use of alginate for cell immobilization
 DOWNSTREAM PROCESSING LAB
Course Code: BTB 721 Credit Units: 01
Module I
Conventional filtration and membrane based filtration
Module II
Protein precipitation and recovery
Module III
Aqueous two-phase separation
Module IV
Ion exchange chromatography
Module V
Gel Permeation chromatography
Module VI
Electrophoresis

GENOMICS AND PROTEOMICS LAB

Course Code: BTB 722 Credit Units: 01
Module I
Three dimensional Structures – In silico study – large molecular complexes RNA polymerase II, ribosome,
unstructured proteins
Module II
DNA sequencing methods
Module III
Gene finding tools and Genome annotation
Module IV
Comparison of two given genomes
Module V
Analysis of 2D – IEF data
Module VI
Microarray and Microarray data analysis
Module VII
Inference of protein function from structure
Module VIII
Inference of protein function and structure
Module IX
Two-hybrid methods

COMMUNICATION SKILLS - V
Course Code: BTB 741 Credit Units: 01
Module I
Introduction to Public Speaking
Business Conversation
Effective Public Speaking
Art of Persuasion
Module II: Speaking for Employment
Types of Interview
Styles of Interview
Facing Interviews-Fundamentals and Practice Session
Conducting Interviews- Fundamentals and Practice Session
Question Answer on Various Dimensions
Module III
Resume Writing
Covering Letters
Interview Follow Up Letters
Module IV: Basic Telephony Skills
Guidelines for Making a Call
Guidelines for Answering a Call
Module V: Work Place Speaking
Negotiations
Participation in Meetings
Keynote Speeches

FRENCH - VII
Course Code: BTB 744 Credit Units: 02
Module A: Unités 1 – 3: pp. 06 - 46
Contenu lexical: Unité 1: Rédiger et présenter son curriculum vitae
Exprimer une opinion
Caractériser, mettre en valeur
Parler des rencontres, des lieux, des gens

Unité 2: Imaginer - Faire des projets

Proposer - conseiller
Parler des qualités et des défauts
Faire une demande écrite
Raconter une anecdote
Améliorer son image

Unité 3: Exprimer la volonté et l’obligation

Formuler des souhaits
Exprimer un manque/un besoin
Parler de l’environnement, des animaux, des catastrophes
naturelles
Contenu grammatical:
1. Le passé : passé composé/imparfait
2. Pronoms compléments directs/indirects, y/en (idées/choses)
3. Propositons relatives introduites par qui, que, où
4. Comparatif et superlatif
5. Le conditionnel présent
6. Situer dans le temps
7. Féminin des adjectifs
8. La prise de paroles : expressions
9. Le subjonctif : volonté, obligation