This certificate of analysis summarizes the results of testing performed on a batch of stevioside food grade product. The active ingredient is shown to be steviol glycosides with a total content of 96%. All tested parameters including appearance, solubility, pH, ash, loss on drying, residual solvents, heavy metals, and microbiological analysis were within specified limits and standards.
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Stevia Analysis Report
This certificate of analysis summarizes the results of testing performed on a batch of stevioside food grade product. The active ingredient is shown to be steviol glycosides with a total content of 96%. All tested parameters including appearance, solubility, pH, ash, loss on drying, residual solvents, heavy metals, and microbiological analysis were within specified limits and standards.
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Certificate of Analysis
Product Name (Stevioside Food Grade)
Active Ingredient (4a-13-[(2-0-ß-D-Glucopyranosyl-ß-D glucopyranosyl)oxy] kaur-16-en-18-oic acid ß-D- glucopyranosyl ester Batch No 03-10-05S Date of Manufacturing 15.03.2010 Date of Analysis 16.03.2010
Results
Parameter Test Method EAO-JECEFA Result
Standard Assay HPLC as per FAO JECFA Not less than 95% of Total glycoside content 96% Monographs Vol.4 (2007), the total of the seven named steviol glycosides, on the dried basis. Appearance Organoleptic White to light yellow Passas test powder, odourless or having a slight characteristic odour Solubility FAO JECFA Soluble in water and in Passas Test Monographs Vol.4 ethanol (2007) pH (1 in 100 aqueous By single electrode pH mater 4.5 to 7.0 4.88 solution) Total Ash AOAC 18th Not more than 1% 0.32% EDN.:2006
Loss on drying AOAC 18th Not more than 6% 3.5 %
END.: 2006 Residual Solvents GC-MS Head Not more than 200 mg 105 mg MeOH/Kg Space/SGS PC methol/kg IHM-0005A Arsenic AOAC 18th Not more than 1 Not Detectable (Detectable EDN.:2006 mg/Kg limit 0.10 mg/kg) Lead AOAC 18th Not more than 1 Not Detected (Detectable Limit EDN.: 2006 mg/Kg 0.50 mg/kg) Total Aerobic Plate ISO 4833:2003 Less than 1000 Less than 10 CFU/g count CFU/g Coliform ISO 4832:2006 Absent in 10 g Passes Test Salmonella ISO 6579:2002 Absent in 25 g Passas Test