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    Inositol PDF

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    Tio Prima S
    1. The study examines the ability of Rhizobium leguminosarum to utilize myo-inositol, which is abundant in legume root exudates and nodules. 2. Transposon mutants deficient in myo-inositol utilization were outcompeted by the wildtype strain in nodulation of peas and vetch. 3. The ability to utilize myo-inositol appears to confer a competitive advantage for Rhizobium in colonizing the legume rhizosphere.

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    Inositol PDF

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    Tio Prima S
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    1. The study examines the ability of Rhizobium leguminosarum to utilize myo-inositol, which is abundant in legume root exudates and nodules. 2. Transposon mutants deficient in myo-inositol utilization were outcompeted by the wildtype strain in nodulation of peas and vetch. 3. The ability to utilize myo-inositol appears to confer a competitive advantage for Rhizobium in colonizing the legume rhizosphere.

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    1. The study examines the ability of Rhizobium leguminosarum to utilize myo-inositol, which is abundant in legume root exudates and nodules. 2. Transposon mutants deficient in myo-inositol utilization were outcompeted by the wildtype strain in nodulation of peas and vetch. 3. The ability to utilize myo-inositol appears to confer a competitive advantage for Rhizobium in colonizing the legume rhizosphere.

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    Inositol PDF

    Uploaded by

    Tio Prima S
    1. The study examines the ability of Rhizobium leguminosarum to utilize myo-inositol, which is abundant in legume root exudates and nodules. 2. Transposon mutants deficient in myo-inositol utilization were outcompeted by the wildtype strain in nodulation of peas and vetch. 3. The ability to utilize myo-inositol appears to confer a competitive advantage for Rhizobium in colonizing the legume rhizosphere.

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    Degradation of myo-Inositol Profoundly Alters the Competitive Ability of Rhizobium in the Rhizosphere

    J. Fry, P.S. Poole, *M. Wood School of AMS, *Department of Soil Science, University of Reading, Whiteknights, Reading, RG6 6AJ.

    Introduction myo-Inositol is the most abundant sugar/polyol in soil. With arabinose, myo-inositol is the principal sugar in root exudates of hydroponically grown legumes (Stanway and Wood, 1998). Rhizobium leguminosarum biovar viciae forms nodules on Pisum sativum and Vicia sativa, which has an inducible pathway for myo-inositol (Poole et al, 1994) (fig.1). Despite being abundant in legume root nodules, myo-inositol does not appear to be catabolised by Hypothesis We hypothesise that the ability to utilise myoinositol is important for growth and competition in the rhizosphere. myo-Inositol degradation pathway myo-inositol myo-inositol dehydrogenase 2-deoxy-5-keto-D-gluconic acid DKH kinase diketohydroxy phosphate DKHP aldolase 3.

    myo-Inositol catabolic mutants of R. leguminosarum bv. viciae Transposon mutants deficient for growth on myo-inositol have been isolated. Three distinct regions containing genes involved in myo-inositol utilisation have been identified.

    Competition studies between myo-inositol utilisation mutants and wildtype in vivo. The three mutants nodulate Pisum sativum (common pea) and Vicia sativa (common vetch) and fix nitrogen at the same rate as the wildtype (Poole et al, 1994). When co-inoculated in equal numbers onto peas and vetch in sterile conditions, over 90% of nodules recovered contained wildtype bacteria and not the mutants (fig.2). This advantage is still evident when seedlings are inoculated with 100-fold higher doses of the mutant strains than the wildtype (data not shown).

    1.

    Strain RU360 is mutated in an acetolactate synthase gene. Immediately downstream are two other genes thought to be involved in myo-inositol utilisation. Strain RU361 is mutated in a methylmalonic semialdehyde dehydrogenase. This is one of the final enzymes in the degradation pathway. Strain RU307 is mutated in a gene thought to be involved in transport of myo-inositol.

    2.

    Bacteria recovered from Vicia sativa nodules

    total no. nodules recovered per replicate

    100 90 80 70 60 50 40 30 20 10 0

    RU361 RU360 3841

    Expression of promoters induced by myo-inositol Screening of a library of Green Fluorescent Protein (GFP) gene fusions to Rhizobium promoters has enabled identification of promoters induced in the presence of myoinositol. These fusions will be tested to determine whether the promoters are induced in the plant rhizosphere.

    2-keto-myo-inositol 2-keto-inositol dehydratase

    uninoculated

    3841

    RU360 Treatment

    RU361

    3841 + RU360

    3841 + RU361

    D-2,3-diketo-4-deoxy-epi-inositol

    Fig.2

    Conclusions References Poole, P.S., Blyth, A., Reid, C.J., Walters, K. (1994). Microbiology. 140: 2787-2795. Stanway, A.P., Wood, M. (1998). Submitted. pyruvate Plant and Soil. There are at least three distinct gene clusters involved in myoinositol utilisation in R. leguminosarum bv. viciae. These regions appear to be under the control of a common regulator. Data obtained indicate that the ability to utilise myo-inositol confers a profound competitive advantage to Rhizobium for growth or nodulation in the rhizosphere. The effects on rhizosphere colonisation are currently being investigated.

    malonic semialdehyde + dihydroxyacetone phosphate MSA oxidative decarboxylase acetyl CoA + CO2 + NADH

    acetolactate synthase acetolactate Fig.1

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