Genius 3diff Operator Manual

Vec 3 Diff Operation Manual Edition: A/0
Chapter 1 Instrument Introduction ................................................................................... 6 1.1 Name

......................................................................................................................... 6 1.2 Structure
.................................................................................................................... 6 1.2.1 Host
............................................................................................................... 6 1.2.2 Accessory
...................................................................................................... 6 1.3 Purpose
..................................................................................................................... 6 1.4 Specifications
............................................................................................................. 7 1.5 Structure
................................................................................................................... 11 1.5.1 Front Panel
................................................................................................... 11 1.5.2 Rear panel
.................................................................................................... 11 1.6 Operation
.................................................................................................................. 11 1.6.1 Display Screen
............................................................................................. 12 1.6.2 Menu
............................................................................................................ 12 1.7 Detection Principle
................................................................................................... 12 1.7.1 Detection Principles of WBC,
RBC, PLT ..................................................... 13 1.7.2 Principles of HGB Measurement
................................................................. 14 1.7.3 Volume Distribution of Blood Cell
................................................................ 14 1.7.4 Results and Calculation of Parameter Measurement
.................................. 15 Chapter 2 Installation
........................................................................................................ 16 2.1 Package
................................................................................................................... 16 2.2 Unpack
..................................................................................................................... 16 2.3 Installation
Requirements ........................................................................................ 16 2.3.1 Installation Environment
.............................................................................. 16 2.3.2 Space Requirement
..................................................................................... 16 2.3.3 Power Requirement
..................................................................................... 16 2.4 Reagent tubing connection
...................................................................................... 17 2.4.1 Lyse Connection
.......................................................................................... 17 2.4.2 Diluent Connection
...................................................................................... 17 2.4.3 Waste Connection
....................................................................................... 17 2.5 Recorder Paper Installation
..................................................................................... 18 2.6 Keyboard and mouse installation
............................................................................ 18 2.7 Printer Installation (Optional)
................................................................................... 18 2.8 Power Cable Connection
......................................................................................... 18 Chapter 3 Sample Analysis
.............................................................................................. 19 3.1 Preparation Before Startup
...................................................................................... 19 3.2
Startup...................................................................................................................... 19 3.2.1 Function
....................................................................................................... 20 3.3 Background Test
...................................................................................................... 20 3.4 Quality Control
......................................................................................................... 21 3.5 Preparation for sample
collection ............................................................................ 21 3.5.1 Venous Blood Collection
............................................................................. 21 3.5.2 Capillary Blood Collection
............................................................................ 21 3.5.3 Prepare Samples in Prediluted Mode
.......................................................... 22 3.5.4 Prepare Samples in Capillary Mode
............................................................ 22
1
3.5.5 Sample Homogenization ............................................................................. 22 3.6 Sample count and
Analysis ..................................................................................... 22 3.6.1 Input Sample Information
............................................................................ 22 3.6.2 Procedures of Sample Count and Analysis
................................................. 24 3.6.3 Parameter Alarm
.......................................................................................... 24 3.6.4 Histogram Alarm
.......................................................................................... 24 3.7 Analytical Results Modification
................................................................................ 24 3.8 Shutdown
................................................................................................................. 25 Chapter 4 Quality Control
................................................................................................. 27 4.1 Edit Quality Control Files
......................................................................................... 27 4.2 Quality Control Run
................................................................................................. 28 4.3 Quality Control Graph
.............................................................................................. 29 4.4 Quality Control Table
............................................................................................... 30 Chapter 5 Calibration
........................................................................................................ 31 5.1 Operator calibration
................................................................................................. 31 5.1.1 Background Test
.......................................................................................... 31 5.1.2 Assurance of Repeatability
.......................................................................... 31 5.1.3 Calculate Calibration Factors
...................................................................... 32 5.1.4 Modify the Calibration Coefficient
................................................................ 32 5.2 Auto Calibration
....................................................................................................... 33 Chapter 6 Setting
.............................................................................................................. 35 6.1 Date & Time
............................................................................................................. 35 6.2
Printout..................................................................................................................... 35 6.3 Control
..................................................................................................................... 37 6.4
Limits........................................................................................................................ 38 6.5 Department
information setting ............................................................................... 39 6.5.1 Add Record Information
............................................................................... 39 6.5.2 Modify Department Information
................................................................... 40 6.5.3 Delete department information
.................................................................... 41 6.5.4 Print Department Information
...................................................................... 41 6.6 Operator Information
................................................................................................ 41 6.6.1 Add Operator Information
............................................................................ 41 6.6.2 Modify Operator Information
........................................................................ 42 6.6.3 Delete operator Information
......................................................................... 43 6.6.4 Print Operator Information
........................................................................... 43 Chapter 7 Review
.............................................................................................................. 44 7.1 Sample Review
........................................................................................................ 44 7.2 Detail Review
.......................................................................................................... 46 Chapter 8 Service
.............................................................................................................. 48 8.1 Flush
........................................................................................................................ 48 8.2 Enhanced Flush
....................................................................................................... 48 8.3 Cleaning
................................................................................................................... 48 8.4 Drain Chambers
....................................................................................................... 48 8.5
Park.......................................................................................................................... 48
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8.6 Prime chamber ........................................................................................................ 49 8.7 Prime
Dilutent .......................................................................................................... 49 8.8 Prime Lyse
............................................................................................................... 49 8.9 Maintenance
............................................................................................................ 49 8.10 Mechanic
............................................................................................................... 49 8.11 Special
................................................................................................................... 50 Chapter 9 Maintenance
..................................................................................................... 51 9.1 Routine Maintenance
............................................................................................... 51 9.1.1 Clean the Appearance
................................................................................. 51 9.1.2 Replace Fuse
............................................................................................... 51 9.2 Daily Maintenance
................................................................................................... 51 9.3 Weekly Maintenance
............................................................................................... 52 9.4 Monthly Maintenance
............................................................................................... 52 9.5 Yearly Maintenance
................................................................................................. 52 9.6 Maintenance before transport or for
the instrument that will not be used for a long time
.................................................................................................................... 52 Chapter 10 Troubleshooting
............................................................................................ 53 10.1 Abnormal Startup
................................................................................................... 53 10.2 Waste Full
.............................................................................................................. 53 10.3 Diluent Empty
........................................................................................................ 53 10.4 Lyse Empty
............................................................................................................ 53 10.5 WBC Clog or RBC Clog
......................................................................................... 53 10.6 WBC Bubbles or RBC Bubbles
............................................................................. 53 10.7 HGB Error
.............................................................................................................. 53 10.8 HGB Bubbles
......................................................................................................... 54 10.9 Recorder out of paper
............................................................................................ 54 10.10 Recorder too hot
................................................................................................ 54 10.11 The result of background test too
high .............................................................. 54 Chapter 11 Help
................................................................................................................. 55 Appendix 1 Symbol
........................................................................................................... 56 Appendix 2 Specification
................................................................................................. 57 2.1 Reagent
................................................................................................................... 57 2.2 Parameter Information
............................................................................................. 57 2.3 Sampling characteristics
.......................................................................................... 57 2.4 Performance index
................................................................................................... 57 2.5 Background Range
.................................................................................................. 58 2.6 Linear Range
........................................................................................................... 58 2.7 Repeatability index
.................................................................................................. 58 2.8 Pollution rate
............................................................................................................ 59 2.9 Input/Output
............................................................................................................. 59
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How to use the manual

You are welcome to read this manual. This manual includes the in-depth information about
installation, daily operation, calibration, quality control and maintenance. To achieve the optimal
performance, be sure to operate and maintain the instrument according to this manual. If you have
chosen the optional accessories such as printer, Bar Code Scanner, read the related manuals of those.
The data in this manual has been calibrated and is efficient. If the instrument is used in special purpose,
or the operational procedures/methods exceed the regulation of this manual, please contact with the
manufacturer to inquire its validity and applicability. Otherwise the manufacturer will not be able to ensure
the accuracy and validity of the measuring results, and will not perform any obligation for the direct or
indirect results resulting from this action.
Avoidance from potential hazard

User should read the Safety Notice and Operation Notice carefully. There are several safety warning
signs in this manual to help operators avoid hurting themselves or making the instrument damaged,
resulting in incorrect measuring results: . Warning: The instrument must be operated as the operating
procedures, or else, there will be great hazards to both operator and environment.
Caution: Emphasize the operating methods that must be obeyed. Avoid potential hazard
or making the instrument damaged, resulting in incorrect measuring results. Notice: To
emphasize important information. All personnel that may operate, maintain, remove, service the
instrument should read this manual carefully.
Safety notice
To operate the Instrument safely and effectively, be sure to read the following notices first. Operating
the instrument, without following the appointed methods by the manufacturer, may break down the
defensive function of the system, and cause bodily injury or damage the instrument.
➢ Avoid electric shock 1) When the power-on, the unauthorized maintenance personnel should not pen
the instrument.
2) If liquid enters into the instrument or the instrument leakage, please shutoff the power
immediately, and contact with our company’s Customer Service Department or local distributor in due
course. Improper use of the liquid may cause electric shock and result in damaging the instrument. ➢
Defense for biohazard and chemical hazards Improper use of the sample may result in being infected.
Avoid hand touch the sample, reagent and waste directly. When operate the instrument, be sure to wear
gloves to avoid being infected.
If the skin touched the sample, manage it according to operator working standard or
inquiring a doctor to take remedial measures immediately. Use the reagent carefully to avoid hand and
clothes touch directly.
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Once hand or clothes touch, flush the touched area with soap and plenty of water immediately.
If the reagent enters into the eyes incautiously, flush with plenty of water at once, and inquire a doctor
for further treatment.
Be sure not to use flammable dangerous materials around the instrument. Reagents, quality control
serum, some materials in the sample are controlled by Pollution Regulations and Standard for Discharge
of Pollutants. Please abide by the local discharge regulation and inquire related reagent manufacturer.
➢ Prevention of fire and explosion
Operation notice
To operate the instrument safely and reliably, be sure to obey the following notices.
• Purpose
Pay attention to the application range of the instrument statement. Make sure your use does not
exceed the application range.
• Limitation of operating environment The instrument should be installed according to the required
installation environment of the manual. Installation and use out of the appointed range may cause
unreliable results, and make the instrument damaged. Contact with our company’s Customer Service
Department or local distributor if you need to change the state of the instrument.
• Limit of Operator Only trained and authorized personnel by the manufacturer can operate the
Instrument.
• Maintenance and Service Be sure to service and maintain the instrument according to this manual
strictly.
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Chapter 1 Instrument Introduction

1.1 Name
The full name of the instrument is Vec 3 Diff auto hematology analyzer. Protection Grade: IP20.
1.2 Structure
Host and accessory. 1.2.1 Host
Control the process of sample collection, dilution and analysis, including the following units:
1. Power Supply unit. 2. Central control circuit unit. 3. Dilute unit. 4. Display unit. 5. Thermal recorder.
1.2.2 Accessory
Including the following accessories: 1. PS/2 mouse. 2. PS/2 keyboard. 3. Printer (Optional) 4. Touch pen
5. Power cord. 6. Ground wire.
1.3 Purpose
It is used for detecting the parameters of RBC, WBC, HGB and differential counter. The analyzer is
used for the determination of the following 20 parameters and 3 histograms blood .
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1.4 Specifications
• Standard Classification of the Instrument According to anti-electrical shock hazard: Grade II, Pollution 2
According to the defense for deleterious liquid: General Device(Closing device of non-defensive liquid)
According to the recommended disinfection/sterilization method of the manufactory: Disinfectant device
recommended by manufactory.
According to safety degree in condition that using gas mixture of flammable anesthetic gas and air or
gas mixture of oxygen and nitrous oxide: Do not use the equipment in condition that using gas mixture of
flammable anesthetic gas and air or gas mixture of oxygen and nitrous oxide.
According to working status: Continuous Running Equipment.
• Principles of Measurement Blood Cells are counted and sized by Electrical Impedance Method.
Hemoglobin is determined by Colorimetric Method.
• Parameters of Measurement Basic parameters:
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Calculated Parameters:
Sampling Features:
Sample Volume: Venous Blood Mode: 10uL Venous Blood
Capillary Blood Mode: 10uL Capillary Blood Prediluted Mode: 20uL Capillary Blood
Reagent Volumes Required for Single Sample:
Diluent: 26mL Lyse: 0.35mL Venous and Capillary Blood Mode:
WBC/HGB 1:285 RBC/PLT 1:21667 WBC/HGB 1:500 RBC/PLT 1:43333 Cell Counting Aperture Size:
WBC: 100μm RBC/PLT: μm Throughput: Up to 60 samples per hour Working mode: Double counting
pathway +independent HGB detection system Display Liquid Crystal Display (LCD), Touch screen,
resolution: 800*600 Language English Chinese Spanish Indicator 20 Parameters and 3 Histograms
Display μ Alarm Indicator Working Status (including Power) Indicator Light Alarm Interface One power
input socket One printer port One RS232/C serial ports One VGA interfaces Two USB interfaces Two
PS/2 interfaces Recorder Rapid Thermal Recorder: Recording width: 35mm
Paper width: 80mm Precision Specifications of the instrument
Parameter Repeatability
WBC
≤ 5 × 109 / L
RBC
≤ 5× 1012/ L
HGB
≤ 4g / L
HCT
≤5%
PLT
≤8× 109/ L
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Linear Range
Parameters Measuring range
Measurement of the linear tolerance
WBC
0~5.9×109/L 6.0~99.9×109/L
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(±0.3×109)/L ±5%
RBC
0~0.99×1012/L 1.0~9.99×1012/L
(±0.05×1012)/L ±5%
HGB
0~99g/L 100~300g/L
±2.0 g/L ±2%
PLT
0~99×109/L 100~999×109/L
(±8.0×109)/L ±10%
Operating Ambient Temperature: 18°C~35°C Humidity: 10%RH ~90%RH Atmospheric Pressure: 70.0kPa
~106.0kPa Ambient Storage Temperature: -10°C~40°C Humidity: 10%RH ~90%RH Atmospheric
Pressure: 50.0kPa ~106.0kPa This instrument should be placed at a good ventilation area. Electric
Specifications Power Supply: 100~240Vac,50/60Hz
Fuse: F3AL250V Dimensions and Weight Dimensions: 410mm×435mm×472mm Weight: 25kg
1.5 Structure
1.5.1 Front Panel
figure 1-1 1.5.2 Rear panel
figure 1-2
1.6 Operation
Designed according to human engineering and transferring information to users by software windows
and mouse, both are convenient for your operating.
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1.6.1 Display Screen

The display screen is classified four sections:
SYSTEM LOGO SECTION SHORTCUT KEY SECTION
AUXILIARY INFORMATION SECTION SYSTEM TIME
WINDOWS DISPLAY SECTION
➢ SYSTEM LOGO SECTION
Display the Logo of the system./System time ➢
SHORTCUT KEY SECTION To display the function interfaces of the system. ➢ WINDOWS DISPLAY
SECTION
Display various results. ➢ AUXILIARY INFORMATION SECTION
Fault information prompt area 1.6.2 Menu
You can operate the instrument by button. In the blood cell analyzer window, press "Menu" button,
and pops up the main menu window.
figure 1-3 The button functions are as follows: Measure: Enter the
blood cell analyzer window. Calibration: Enter the calibration window to calibrate the instrument.
1) Operator Calibration: Enter the operator calibration window to calibrate the
instrument manually. 2) Auto Calibration: Enter the auto calibration window to adjust the instrument
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automatically. Quality Control: Enter the quality control window to carry out quality control operation.
1) QCE: Enter the QC edit window to edit the QC parameters. 2) QCR: Enter the QC run window to carry
out the selected QC files. 3) QCG: Enter the QC graphs window to browse the selected QC graphs. 4)
QCL: Enter the QC table window to browse the selected QC tables. Setting: Enter the setting menu.
1) Language: Enter the Language setting window to set the language of the
instrument. 2) Time: Enter the time setting window to set the time of the instrument. 3)
Print: Enter the printout window to set the functions such as print or report. 4) Control: Enter the config
window to set the functions such as print, sleep,
clean, and display etc. 5) Limits: Enter the limits window to modify the limits. 6) Department
Info: Enter the department window to set the department and
abbreviation. 7) Doctor Info: Enter the operator window to set the department, abbreviation,
department and operator. 8) Window Color: Select the background color. 9) Screen Calibration: Calibrate
the screen. Service: Enter the service menu.
1) Flush: To get rid of the aperture clog. 2) Enhanced Flush: To get rid of hard aperture clog. 3) Cleaning:
Run the normal rinse process to rinse the measurement unit. 4) Prime Chamber: Inject the solutions into
the chambers 5) Drain Chambers: Empty the liquid of the counter pool. 6) Park: Run the Park Program to
rinse and empty the tubing. 7) Prime Diluent: Inject the diluent into the appointed tubing. 8) Prime Lyse:
Inject the lyse to the appointed tubing. 9) Prime: inhale the solutions 10) Maintenance: Run the
maintenance program to maintain the instrument. 11) Mechanic: Enter the Mechanic window to verify the
functions of the
instrument. 12) Special: Enter the Special window to maintain the instrument. Review:
Enter the review menu. Help: Access the system help window. About: Display information of the
instrument such as software and copyright etc. Shutdown: Startup the shutdown program to rinse the
measurement unit
automatically.
1.7 Detection Principle

1.7.1 Detection Principles of WBC, RBC, PLT
The count principle of the instrument is based on the measurement of changes in electrical resistance
produced by a particle passing through an aperture sensor.
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a) The sample blood is diluted in a conductive liquid. As blood cells are non-conductive, the diluent is
a good conductor. There are big differences between them.
b) When the diluent passes through the aperture sensor, electrodes are submerged in the liquid on
each side of the aperture to create continuous current.
c) When cells pass through the aperture, the resistance between the electrodes increases as the cell
volume increasing, as shown in figure 1-4.According to the Ohm Formulary: U=RI (U=Voltage I=Current
R=Resistance). If I is a constant, U increases as the cell volume increasing.
d) Passing through the magnification circuit, the voltage signal will be magnified and the noise will be
filtered, then you will gain the analytical results, as shown in figure 1-4.
e) One count bath and the detection circuit count the WBC. Another count bath and the detection
circuit count the RBC and PLT. The microprocessor of the instrument calculates and analyzes the cells
(WBC, RBC, PLT), and then gives out the histograms.
f) PLT count adopts advanced liquid, electronics and software system. It settles the repetitive count of
the cells on the side of the aperture count area.
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1.7.2 Principles of HGB Measurement

Adding lyse in the blood, the red blood cell will rapidly be broken down and release hemoglobin.
Hemoglobin and lyse form a new mixture, which can absorb the wavelength of 540nm.Measure the
absorbency. Through the comparison of the absorbency between the pure diluent and the sample the
concentration of the sample hemoglobin is calculated.
1.7.3 Volume Distribution of Blood Cell

When different types of cells pass through the aperture sensor, there will be different electrical pulse
height. Because of the evident difference of the cells sizes, the instrument is able to differentiate the white
blood cell, red blood cell and platelet by its pre-set program.
The volume distributions are as follows:
WBC: 20_1000Fl RBC : 82_98fL
PLT: 2_35fL The leucocytes disposed by lyse can be divided into
three types, according to their volumes: lymphocyte (LYM), mid-sized cell (MID), Neutrophil (NEUT).
LYM: 35-90 fL MID: 90-160fL NEUT: 160-450fL 1.7.4 Results and Calculation of
Parameter Measurement
The parameters of the sample blood test can be described in three ways: a) Measured directly, such as
WBC, RBC, PLT, and HGB. b) Derived from histograms, such as LYM%, MID%, NEUT%, MCV, RDW-
SD,
RDW-CV, MPV, PDW, P-LCR. c) Calculated, such as LYM#, MID#, NEUT#, HCT, MCH, MCHC,
PCT. The derivation of the formularies as follows: MCV derives from histograms and the instrument
collecting and classifying the erythrocytes according to its volume. It is determined by measuring the
average volume of individual erythrocytes, and the unit is fL.
RDW represents the volume distribution of the erythrocyte populations, derived from the RBC
histogram. It can be expressed by the coefficient variation of the erythrocyte Volume as RDW-CV and the
unit is %.It can also be expressed in standard deviation of the erythrocyte volume as RDW-SD, the unit is
fL.
MPV is the average volume of individual platelets, derived from the PLT histogram. It represents the
mean volume of the PLT populations and can be expressed in fL.
PDW derives from the PLT histogram. It represents the geometry standard deviation (10 GSD) of the
volume of the PLT populations.
P-LCR derives from the PLT histogram. It represents the ratio of the larger PLT and the unit is %.
• HCT (%) = RBC*MCV/100
• MCH (pg) = 10 × HGB/RBC
• MCHC (g/L) = 100 ×HGB/HCT
• PCT (%) = PLT ×MPV/100
• LYM%=100×AL/(AL+AM+AG)
• MID%=100×AM/(AL+AM+AG)
• NEUT%=100×AG/(AL+AM+AG)
• LYM#=LYM%×WBC/100
• MID=MID%×WBC/100
• NEUT#=NEUT%×WBC/100 AL: Number of cells in LYM area; AM: Number of cells between
lymphocyte and Neutrophil area; AG: Number of cells in NEUT area.
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Chapter 2 Installation
2.1 Package
If packing damage is discovered after receiving the instrument, or the instrument is badly damaged,
contact with the freight agent immediately to file a claim according to the damage level. At the same time
contact with your supplier to make sure the packing is complete. Then unpacking and installing the
instrument as the following steps.
2.2 Unpack
Take out the instrument and accessories from the packing case carefully. Preserve the packing
material for future transportation or storage.
1. Check the accessories according to the packing list. 2. Check whether there are mechanical damages
on the instrument and accessories IF there is any problem, please recover the package and contact with
your supplier immediately.
2.3 Installation Requirements

2.3.1 Installation Environment
The instrument should be placed on a clean steady platform. Keep away from direct sunlight and dust.
Temperature: 18~35°C Humidity :≦70% Atmospheric pressure : 70.0~106.0Kpa. Caution:
The instrument should avoid direct sunlight. Caution:
The working environment of the instrument should avoid powerful equipment such as Centrifuge, CT
machine, NMR equipment, X-ray machine etc Caution:
Do not use equipment that may create strong radiation field such as mobile phone and cordless
telephone. Strong radiation field will disturb some functions of the instrument.
2.3.2 Space Requirement

At least 20cm on each side is the preferred access to ensure good airiness Notice:
Reagents must be placed at the same working height as the instrument. 2.3.3 Power Requirement The
power requirement as follows:
• AC 100~240Vac, 50 Hz/60 Hz
• The maximum power consumption is 200VA Warning:
The instrument requires a single well-grounded power socket. Its grounding voltage is no more than
0.5V Warning:
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A grounded outlet is required to connect with the grounding pole on the rear panel. Make sure of the
reliability of the power grounding at working site. Caution:
Fluctuating electric will badly decrease the performance and reliability of the instrument. Proper action
such as the installation of UPS (self-preparation) should be taken before use.
2.4 Reagent tubing connection

There are three tube connectors with color of white, green, red on the rear panel of the instrument.
2.4.1 Lyse Connection

Take out the lyse tube with red connector from the accessory kit. Connect the red lyse tubing
connector with the tubing connector of the same color on the rear panel of the instrument.
Place the other end of the lyse inlet tubing into lyse container, and turn the container cover until
secure.
2.4.2 Diluent Connection

Take out the diluent tube with green connector from the accessory kit. Connect the green diluent
tubing connector with the tubing connector of the same color on the rear panel of the instrument.
Place the other end of the diluent inlet tubing into diluent container, and turn the container cover until
secure.
2.4.3 Waste Connection

Take out the waste tube with white connector from the accessory kit. Connect the white waste tubing
connector with the tubing connector of the same color. Connect BNC plug with BNC socket marked
"WASTE" on the rear panel of the instrument Turn the waste container cover clockwise until secure.
Caution:
After completing all the tubing installation, keep the tubing natural state, without distortion, folding, and
twist. Caution:
All the tubing connectors must be installed manually. Forbid using any tool. Caution:
The reagent must be applied with the instrument. Otherwise it may easily cause inaccurate
measurement results and incorrect classification of the blood cell, or make the tubing system badly
damaged. Caution:
Keep the reagent from direct sunlight. Caution:
After replacing the reagent container, discard the rest of the reagent in the former container. Forbid
putting the rest of the reagent into the replacing container. Avoid polluting the new replacing reagent.
Caution:
When replacing the reagent, avoid making the plastic tube (inserted into the reagent container) contact
other things. Avoid polluting the new replacing reagent.
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Caution:
Avoid using frozen reagent. Caution:
Avoid using the reagent out of its expiration date. Warning:
Handle and dispose of the waste according to acceptable laboratory, local state and national
standards.
2.5 Recorder Paper Installation

a) Tear down the gummed paper from the door of the recorder. b) Gently press the door of the recorder to
open it. c) Insert the new paper into the paper entry, and make the printing side towards the thermal
head.
d) When the paper juts out from the other side, pull it out and keep it straight. e) Pull the paper out of the
paper exit.
2.6 Keyboard and mouse installation

Take out the keyboard, mouse cushion and mouse from the packing case carefully.
Plug keyboard cable into the interface marked
Plug mouse cable into the interface
marked
on the rear panel of the instrument. on the rear panel of the instrument. The keyboard and
mouse can be placed where it is convenient for your operating. We suggest that the keyboard under the
display screen of the instrument, the mouse cushion at the right side of the keyboard, and the mouse on
the mouse cushion.
2.7 Printer Installation (Optional)

Take out the printer from the packing case carefully. Install the printer according to the printer manual.
NOTICE: The printer cable can only be connected with the interface marked
on
the rear panel of the instrument.
2.8 Power Cable Connection

Make sure the power switch is off (0) on the rear panel of the instrument. Insert one end of the power
cable into the power cable interface on the instrument, the other end into the power socket.
Connect the ground wire to the grounding port on the instrument. Warning:
Ensure the power is suitable for the instrument before connection.
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Chapter 3 Sample Analysis

3.1 Preparation Before Startup
Every time before startup, the operator should do the following checks: a) Whether the diluent and waste
tubing are distortion, folding, twist, and whether the
connection is secure. b) Whether the power cable connection is secure. c) Whether the accessory
connection is secure.
3.2 Startup
If external printer is equipped, turn on the printer power r and ensure they are in ready status.
Press the power button on the rear panel of the instrument, then the power lamp on the front panel of
the instrument lights on. The instrument automatically performs initialization program.
After completing initialization, the instrument will access self-test window. It tests the working
conditions of each part and if there is enough diluent, and lyse. Simultaneously prime and clean the
tubing.
After self-test, the instrument will access blood cell analyzer window, as shown in Figure If the system
detects malfunction, the failure information will be displayed on Information Section.
Figure3-1 The button functions are as follows: Info: Enter the
information window of the next sample, input and modify the information. Flush: To get rid of the aperture
clog. Print: Output the data of the current sample to the appointed device. Menu: Enter the main menu
window.
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3.2.1 Function
In the blood cell analyzer window, press "func" button, enter the “function“ window.
figure 3-2 The button functions are as follows: Sample Type:
Choose the sample mode among Venous Blood Mode, Capillary Blood Mode and Prediluted Mode.
Diluent: Inject the diluent quantificationally by sample needle. This menu uses only in Prediluted Mode.
Histogram: Enter the histogram adjustment window of the current sample. You can manually adjust the
histograms of the current sample.
3.3 Background Test

Background test is recommended after the instrument startup normally every time. The operator can
also perform background test in need. The operating procedures as follows:
a) In the blood cell analyzer window, press "Info" button, and pops up the information edit window.
Then click the item textbox, change the ID as 999.then press "OK", return to blood cell analyzer window.
b) In venous or capillary mode, press the "START" key below the sample needle directly for
background count. In prediluted mode, the sample needle aspirates some non-contaminated diluent from
the sample cup for background count. The diluent pick-up method as follows(Analysing the sample in
prediluted mode, it is also necessary to dilute sample with the diluent which is prior picked-up in this
method):
1) Press the “function” button , select "Sample Mode"; Set up Prediluted as the sample
mode. 2) In the blood cell analyzer window , Press “function” button ,.In menu operation area,
select "Diluent"; 3) Place a clean empty sample cup or tube under the sample needle, press
"START" key to dispense the diluent into the sample cup. Press the "START" key once, the diluent is
dispensed into the sample cup or tube once. (The quantity can be used for background count
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in the second prediluted mode). Press "Exit" button, the instrument return to main operation area c) The
permissive range of the background test results are shown as table 3-1.
Parameters Data
WBC
≤ 0.3 × 109 / L
RBC
≤ 0.03× 1012/ L
HGB
≤1g/L
HCT
≤ 0.5 %
PLT
≤ 5× 109/ L
If the value of the background test exceeds the permissive range, repeat the upper testing procedures
until the testing result is acceptable. If the test has been carried on above five times, the testing result still
cannot reach the standard required. Please refer to Chapter 10 “Troubleshooting" in this manual. Notice:
Only five parameters WBC, RBC, HGB, HCT, PLT can be tested and displayed in background test.
Notice:
The serial number 999 is a special number for background test.
3.4 Quality Control

It is necessary to perform quality control in installation for the first time or before sample analysis every
day. Further information and procedures are specified in Chapter 4 "Quality Control".
3.5 Preparation for sample collection

The sample can be collected either from capillary blood or from venous blood.
3.5.1 Venous Blood Collection

It is possible to collect venous blood by using negative pressure tube or in common collecting method.
Add some anticoagulant in advance to the container for venous blood collection. EDTA.K
2
.2H
2
O is commonly used as the anticoagulant, whose specified content is 1.5—2.2mg/ml blood.
3.5.2 Capillary Blood Collection

Parts for blood collection: Adult: Middle finger tip inner of the left hand, or ring finger Child: (above six-
month-old): tip inner of the left hand Infant: (under six-month-old): Middle finger Outer side of the thumb
or foot 1. Gently massage the blood collection part to make it congest naturally, wipe the
partial skin with tampon containing 75% alcohol, then air. 2. Pinch the puncturing part, and
puncture it with aseptic sample needle quickly. The
puncturing depth is about 2-3mm. 3. Wipe off the first drop of blood, and then start collection.
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4. Press the wound with tampon for a moment after finishing the collection. Notice: The capillary blood
collection should follow the professional capillary blood collection standard. The general method is partial
centesis. The typical collection is puncturing from the tip of the finger. Caution:
If the blood flows not freely, press far away from the wound, not around the wound. Avoid the make
the histiocyte mixing into the blood, resulting in incorrect analytical result.
3.5.3 Prepare Samples in Prediluted Mode

a) In the blood cell analyzer window, Press the “funcion” button , select "Sample
Mode";Set up Prediluted as the sample mode. Then Press “funcion” button ,.In menu operation area,
select "Diluent"; b) When the "Diluent" pops up, place a clean sample cup obliquely under the probe.
Press "START" key. The instrument starts to add diluent quantificationally.. Scrape the drops at the top of
the sample needle into the sample cup ,Collect 20uL capillary sample in pipette. Wipe the blood attached
on the outer layer of the pipette with clean tissues. Immediately mix up the sample in the diluent sample
cup. Caution:
When adding the diluent, the sample cup must be placed obliquely under the sample needle, which
allows the diluent to run down the cup wall without forming air bubbles.
Notice:
If there is a need to prepare samples in quantities in prediluted mode, user can use the "Diluent"
function to continuously prepare the diluent.
3.5.4 Prepare Samples in Capillary Mode

Collect 40-L capillary sample in pipettes. Immediately mix up the sample in anticoagulated warhead
sample cup. You can also add the capillary blood to the anticoagulated warhead sample cup directly.
3.5.5 Sample Homogenization

The blood samples must be fully homogenized before use. The recommended method is: Shake up
the tube up and down 3-5 minutes, blood mixer is recommended to make the sample be fully
homogenized and ensure the accuracy of measurement. Caution:
If the sample has been placed for a long time or mixed inadequately, it will easily cause measurement
error and incorrect testing results. Caution:
Avoid shaking up the pipette violently Caution:
The sample to be tested can only be stored at room temperature, and the test must be finished within
4 hours.
3.6 Sample count and Analysis

After finishing sample collection, perform count and analysis procedure as follows.
3.6.1 Input Sample Information In the blood cell analyzer window, click "Info" button, pops up
information edit window. Move
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the cursor to the required inputting item textbox. Input or select the data, and press "OK". The instrument
will save the input information and returns to blood cell analyzer window. Pressing "Cancel" key, the
instrument cancels the input information and returns to blood cell analyzer window. Name: Maximum 19
letters. Sex: Select male or female, and the default is blank. Age: Choose from year, month and day.
Maximum 3 numbers while choosing year and day; Maximum 2 numbers while choosing month. Blood:
Choose from . A, B, O, AB, A RH+, B RH+, O RH+, AB RH+, A RH-, B RH-, O RH-, AB RH-, The default
is blank. Sample: The current sample mode. Case Hist: Maximum 15 letters. Bed No.: Maximum 12
letters. ID: The input range is: 001 --998. The instrument owns an intelligent number manager. The final
number derives from the comprehensive information that the instrument adds the information of year,
month and day in front of the input number according to the measured time. If user does not input the
serial number, the instrument will accumulate the number from 001 everyday according to the measured
time. Department:.
You can also choose the recorded department information from the right optional textbox, or choose
automatically by the instrument after inputting the code of the department in the left input textbox. Send
By: You can also choose the recorded doctor information from the right optional textbox, or choose
automatically by the instrument after inputting the code of the doctor in the left input textbox. Operator:
You can also choose the recorded doctor information from the right optional textbox, or choose
automatically by the instrument after inputting the code of the doctor in the left input textbox. Checker:
Maximum 19 letters. You can also choose the recorded doctor information from the right optional
textbox, or choose automatically by the instrument after inputting the code of the doctor in the left input
textbox. Limits:
Choose from general, men, woman, child, baby, define 1, define 2, and define 3 automatically. While
choosing "Auto", the instrument gives the reference value automatically as shown in table 3-2. Notice:
The number 999 is a special number for background test. Please don't input this number in blood
sample test. Notice:
If there is a need to input the sample test information in quantities, user can concentrate on inputting
the sample test information in the review window after the current sample test finished.
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3.6.2 Procedures of Sample Count and Analysis

a) Place a sample cup under the sample needle, press "START" key, the indicator lamp on the front
panel of the instrument begins to glimmer. The instrument starts to aspirate the liquid. Move the sample
cup until the indicator lamp stops glimmering.
b) The instrument starts to analysis the sample automatically. Please wait for the analytical result.
c) After the analysis finished, the result will be displayed in the rear of the corresponding parameters
on blood cell analyzer window of the instrument, with histograms WBC, RBC, and PLT. As shown in
figure 3-1. If "Auto" item is "ON" , the recorder or printer will output the test results automatically. If there
are clogs or bubbles during the count and analysis process, the information section would display "Clog"
or "Bubble". 3.6.3 Parameter Alarm
"B": To indicate that bubble appears in the test. "C": To indicate that clog appears in the test.
"↓": To indicate the data has exceeded the setup lower alarm limit of the parameter. "↑": To indicate the data has
exceeded the setup higher alarm limit of the parameter. "***": To indicate that the data is invalid. Notice:
When PM alarm occurs on PLT histogram, the result of parameter PDW is "***". Notice:
If the result of WBC is less than 0.5×10 /L, the system will not perform leukocyte differential. The
display for all parameters related to leukocyte differential is "***".
3.6.4 Histogram Alarm
"R1": To indicate the abnormality of the lymphocyte hump on the left. It may present platelets coagulate,
large platelet nucleated red cell, insolvable red cell, abnormal lymphocyte, protein etc.
"R2": To indicate the abnormality between the lymphocyte and the mid-sized cell area. It may present
atypical lymphocyte, abnormal lymphocyte, plasma cell, original cell or the increase of the number of the
eosinophil or basophil.
"R3": To indicate the abnormality between the mid-sized cell and Neutrophil. It may present immature
Neutrophils, abnormal sub-population in the sample, or the increase of the number of the eosinophil.
"R4": To indicate the abnormality on the right of Neutrophils area. It shows the increase of the number
of the Neutrophil.
"RM": To indicate that more than two areas occurs abnormality. The upper reasons exist
simultaneously.
"PM": To indicate the abnormality the blur demarcation between platelet and red blood cell area. Large
platelet, platelet coagulation, small red blood cell, cell debris or fibrin may exit.
3.7 Analytical Results Modification

If user thinks that the results of WBC, RBC, PLT differential can not meet the needs of the
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classification of special samples in clinical or laboratory, he can manually adjust the histograms.
Operate as follows: a) In the blood cell analyzer window, select "function" in menu. Then press
“Histogram” The instrument accesses histogram adjustment window, as shown in figure 3-2. Press "Para"
button to adjust the histogram.
b) After selecting the histogram, press "Line" button, and select the sorting line you want to adjust.
c) Press "Left" or "Right" button, you can remove the sorting line to the left or right, the data of the line
will be displayed at the top right corner of the screen.
d) Press "back" button when the adjustment finished. If this operation did not change any data, the
system would return to the blood cell analyzer window directly. Or else pops up the "OK/Cancel" window.
Press "OK" to save the adjustment result, while select "Cancel" to cancel the adjustment result. Caution:
Unnecessary and incorrect manual adjustment will cause unreliable analytical result. Make sure the
necessity of the operation. Notice:
If the result of WBC is less than 0.5D10 /L, the system will not perform leukocyte differential
automatically.
figure 3-3
3.8 Shutdown
Shutdown routine must be performed before turning off the power everyday. During shutdown procedure,
the instrument will perform daily maintenance and clean the tubing automatically. The procedures are:
a) In the blood cell analyzer window, press ”MENU” button. in the window,
Select "Shutdown" button, pops up shutdown window
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b) If you do not want to shutdown the instrument for the moment, click "Cancel" to
return to MENU window. c) Press "OK", the instrument will perform daily maintenance and clean the
measuring tubing. When the shutdown procedure finished, the screen displays "Turn off the power now",
turn off the power on the rear panel of the instrument. d) Turn off the printer power (if equipped), clean the
workbench and dispose the
waste.
Notice:
Do not turn off the power of instrument directly while performing the shutdown procedure.
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Chapter 4 Quality Control

Quality Control means the precision, accuracy and repeatability of the system. Quality control provides
reliable and effective methods for the possible system errors in detection and prevention. The system
errors may cause unreliable analytical result of the sample. To maintain the reliability of the analytical
results, periodic quality control of the instrument is required.
The instrument provides 9QCfiles for user. It allows the operator run quality control to the 12
parameters at the same time.
At first select a QC file, enter the assay and limit of control data. The system allows the operator run
quality control to the 12 parameters simultaneously or to some of the 12 parameters. Caution:
Controls must be stored in appropriate conditions. Caution:
Don not use controls deteriorated or out of its expiration date. Caution:
Be sure to finish the quality control in a certain time every day.
4.1 Edit Quality Control Files

The operating procedures as follows: a) In the “MENU” window, press” QUALITY CONTROL” button, in
the window
Select "QCE" in the menu. The instrument enters quality control edit window, as shown in figure 4-1.
figure 4-1.
b) In quality control edit window, select the QC file you want. c) Input Lot No and Exp. Date of the control,
the assay and limit of the quality control parameters.
- 27 -
d) Press "Save" button to save the data of the current QC file. e) Press "Del" button to delete the data of
the current QC file. f) Press "Back" button to save the data of the current QC file and return to the main
menu window. Notice:
If the parameter is an invalid data, the system will take the assay and limit as no assignment.
It needs to re-input. Or else the quality control will only act on other parameters. Notice:
If the lot number or expiration date is invalid, the system will take them as no assignment. It needs to
re-input. Or else the QC file can not run quality control program.
4.2 Quality Control Run

Input QC parameters and perform QC run of the specified file. The operating procedures as follows: a) In
the “menu” window, press” QUALITY CONTROL” button, in the window
Select "QCR" in the menu and select the QC file you want. The instrument enters quality control run
window, as shown in figure 4-2. b) Prepare the controls and shake up the container until it well-mixed.
figure 4-2. c) Place the controls under the sample needle. Press
"START" key, the indicator lamp start to glitter on the front panel of the instrument, the instrument imbibes
the controls, move the controls away until the indicator lamp stops glittering.
d) When the measurement finished, the result will be displayed on the column of the current window. If
alarm occurs in measurement, the current result may be incorrect. Press "Del" button to delete the result
and test again after the problem is solved.
e) Press "Back" button, the instrument will return to blood cell analyzer window. Notice:
Each QC file can store up to 31 QC data.
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4.3 Quality Control Graph

The graph provides a visual observation of the control data, allowing the operator to observe the
possible deviations, trends or shifts of the instrument performance. In the ”menu” window, press”
QUALITY CONTROL” button, in the window, Select "QCG" and QC file you want. The instrument enters
quality control graph window, as shown in figure 4-3.
figure 4-3. The screen can display 1QC figures at the same time.
The figure shows the lot number, time, number and distribution of the QC data. Click the scroll bar on the
right of the screen. continuously select the QC graphs of different parameters. The seven parameters at
the left side of the QC graphs from top to bottom:
assay +3limit assay + 2limit assay + limit assay assay - limit assay - 2limit assay -3 limit The three
parameters at the right side of the QC graphs from top to bottom:
Mean: average value Diff: standard deviation CV: coefficient of variation The vertical line in the middle of
the QC graphs indicates the current chosen number. Click the scroll bar on the left of the screen. You can
continuously select the different number. The chosen number is displayed in the data box below the
corresponding parameter. The test time for the chosen number is displayed in the time box on the screen.
In the QC graphs window, the following marks represent:
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The mark "*" between the broken line represents that the point is within control range. Otherwise it is
out of control range. The mark "." represents that the parameter value is outside the operating range, or
error has occurred during the run. Blank represents that there is no quality control on running. Press
"Print" button, it will print the data of the parameters which you select. Press "Back" button to return to the
blood cell analyzer window.
4.4 Quality Control Table

The instrument supplies tabulated format for QC review. In the “menu” window, press” QUALITY
CONTROL” button, in the window, Select "QCL" and QC file you want. The instrument enters the quality
control table window, as shown in figure 4-4.
figure 4-4. The screen can display the 12 QC parameters of 7
groups at the same time. Operate the scroll bar in to select different serial number.
Press "Print" button, it will print the data of the parameters on the screen. Press "Back" button to return to
the main menu window.
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Chapter 5 Calibration
The instrument has been calibrated strictly at the factory. You may need to perform calibration
procedures when you replace any component that involves the primary measurement characteristics, or
when the shift occurs during controls. Calibration of the instrument is to provide the assurance that the
instrument is providing results with accuracy as design. The purpose of calibration is to ensure the
accuracy of the measurement result meet the requirement of the design all the time. To ensure the
accuracy of the instrument and obtain reliable measurement results, it is necessary to calibrate the
instrument in the following situations:
a) Installation for the first time or re-setup in a new place. b) The instrument is maintained. c) The result of
quality control is abnormal. d) Replace reagent. To ensure the instrument's precision and obtain reliable
measured results, the instrument should be calibrated in these situations: Warning:
Calibrators should be stored and used properly according to the calibrator manual. Warning:
Make sure the instrument is in a normal state before calibration. Warning:
Avoid using the result of the measurement on medical test or clinical before the calibration is finished
accurately. Check the instrument and reagents carefully before calibration. Make sure the instrument in
normal status, and make sure the required sample mode of the measurement.
5.1 Operator calibration

5.1.1 Background Test
Refer to Chapter 3 "Sample Analysis" / Section 3.3, Ensure that the background test meets the
requirement and no malfunction occurs. 5.1.2 Assurance of Repeatability
To ensure the calibration accurate, it is necessary to evaluate the repeatability measurement of the
instrument. Make sure the instrument is in normal status, then perform calibration program. The
procedures are as follows:
a) In the blood cell analyzer window, Measure repeatedly with the calibration (no less
than 3 times). b) Record the data of WBC, RBC, HGB, MCV, PLT. Calculate the CV value according
to the following formula. Only the result is in the limit of table 5-1 can perform calibration.
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- 32 -
5.1.4 Modify the Calibration Coefficient

a) In the blood cell analyzer window, press ”menu” button, in the window, Select " Calibration" in the
menu, then select “operator calibration” button, enters the calibration window, as shown in figure 5-1.
b) Select the calibration data you need from the calibration data menu box. c) Press "Print" button. The
instrument will print the current calibration result. d) Press "OK" button to save the current calibration
result and return to the main menu window.
e) Press "Cancel" button. The instrument will cancel the current calibration result and
return to blood cell analyzer window.
5.1.3 Calculate Calibration Factors

Calculate the new calibration factors according to the following formula:
figure 5-1
5.2 Auto Calibration

The procedures are as follows:
a) In the blood cell analyzer window, press “menu” button, i n the window, Select “Calibration" in the
menu, then select “Auto calibration” button, enters the calibration window, as shown in figure 5-2.
figure 5-2 b) Input the reference value of each calibration
parameter. c) Prepare the calibrators and shake up the container until it well-mixed. d) Place the
calibrators under the sample needle. Press "START" key, the indicator lamp start to glitter on the front
panel of the instrument. The instrument imbibes the calibrators. Move the calibrators away until the
indicator lamp stops glittering.
- 33 -
e) When the measurement finished, the result will be displayed on the column of the current window. If
alarm occurs in measurement, the current result may be incorrect.
f) Press "Del" button to delete the result and test again after the problem is solved. g) Press "Back"
button, the instrument will return to the blood cell analyzer window. Notice:
Calibration can be performed 5 times at the most. Notice:
The calibration result will be displayed after testing 3 times. Notice:
If the parameter is an invalid data, the system will not perform calibration. It needs to re-input valid
data.
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Chapter 6 Setting
The instrument has accomplished all the settings at the factory. For customer's convenience, most of
the system parameters can be setup by the operator so as to meet various requirements. Notice:
The operations in this chapter will change the running state of the instrument. Please make sure the
necessity of the changing before operating.
6.1 Date & Time

In the blood cell analyzer window, press “menu” button, in the window, Select " setting" in the menu,
Select " Time". The instrument enters the time setting window. As shown in figure 6-1. Click the menu bar
to adjust setting item. Press "OK". The instrument will save the current parameters and return to the blood
cell window. Press "Cancel" button, the system will not save the current parameters and return to the
blood cell window.
figure 6-1.
6.2 Printout
In the blood cell analyzer window, press “menu” button, in the window ,Select " setting" in the menu,
Select Print". The instrument will enter the print window. As shown in figure6-2;
- 35 -
:
figure 6-2. Click the menu box you want to adjust, pops up the
optional window .Click the item you want to set.
Press "OK", the instrument will save the current parameters and return to the blood cell window. Press
"Cancel" button, the system will not save the current parameters and return to the main menu window.
➢ Print select Select Recorder, the data will only be transported to the recorder. Select Serial port, the
data will only be transported to net. Select Recorder and Serial port, the data will be transported to the
recorder and net at the same time.
Select Laser Jet, the data will be transported to the HP Laser Jet,1018 printer or HP Laser Jet1008
printer
Select Desk Jet, the data will be transported to the HP desk Jet,1668printer or HP Laser Jet
2668printer
Select Network print, the data will be transported to the computer which connect with HP Laser
Jet1008 printer Notice:
There are different formats when selecting different printer settings.
➢ Print caption Can easily printout the headline on the report.
➢ Print type User can choose the type of the printer. Notice:
The selected printer type must be accord with as the used one, otherwise it will not be able to print
normally.
- 36 -
6.3 Control
In the blood cell analyzer window, press “menu” button, in the window, Select "setting" in the menu,
Select " Control", enter the control setting window. As shown in figure 6-3.
figure 6-3. Click the menu box you want to adjust, pops up the
optional window .Click the item you want to set.
Press "OK", the instrument will save the current parameters and return to the blood cell window.
Press "Cancel" button, the system will not save the current parameters and return to the main menu
window. ➢ Auto Set "Print" as "ON", the system will print the test report automatically after the sample test
finished.
Set "Dorm" as "OFF", the instrument will keep working status all along. Set "Dorm" as data. When the
time, which the user does not perform any operation, exceeds the setting time, the instrument will access
sleep state. During sleep time, the instrument will turn off part of the circuit power, and take the sample
needle back. Sleep function can greatly save electrical energy and extend the life of the instrument. Press
the left key of the mouse or any key on the keyboard, sleep will be canceled.
Set "Clean" as "OFF", the instrument will not perform auto-cleaning. Set "Clean" as data. When the
running time of the instrument exceeds the setting time, the instrument will perform "Clean" procedure
once. It can ensure the measured tubing of the instrument clean and prevent the possible measurement
malfunctions and maintain the instrument.
➢ Sound Set "Alarm" as "OFF". When the instrument appears alarm, it will not make alarm sound, but
display the alarm information in the Information Section.
Set "Alarm" as "ON". When the instrument appears alarm, it will make alarm sound and display the
alarm information in the Information Section.
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Set "Prompt" as "OFF". When the sample measurement finished, the instrument makes no sound.
Set "Prompt" as "ON". When the sample measurement finished, the instrument will make a prompt
sound like "di".
➢ Display Set "Para" as "English". In parameter area of the blood cell analyzer, the parameters will be
displayed in abbreviations.
Set "Limits" as "No Display". In parameter area of the blood cell analyzer, the parameters will not
display reference value of the current sample.
Set "Limits" as "Low To High". In parameter area of the blood cell analyzer, the reference values will
be displayed from low to high.
Set "Limits" as "High To Low". In parameter area of the blood cell analyzer, the reference values will
be displayed from high to low.
6.4 Limits
Select " Limits", enter the limits setting window. As shown in figure 6-4.The 8 Group reference values are
displayed at the left side of the screen. The dot in front of the reference value represents that this is the
current selected reference group.
The limits of the reference value are displayed on in the limits boxes on the screen. Click the dot in
front of the group to select the group required for setting.
figure 6-4. Click "Default" button, the instrument will select the
default reference values to replace the current reference values. Click the adjustable bar of the limits box
to adjust the upper and lower limit of the reference value.
Function of menu and shortcut keys: Save: Save the reference values of the current group.
- 38 -
Print: Print the reference values of the current group. Back: Quit from current window, and return to main
menu window. Notice:
The default reference value may not suitable for the local conditions. User can modify it according to
the local specific circumstances. Caution:
The changing of the reference value will cause variation of abnormal prompt on hematology target.
Please make sure the necessity of the variation.
6.5 Department information setting

The department information can help user setup perfect sorting information in advance, and quicken the
input speed and management of the sample information. Especially the abbreviation greatly improves the
communication and standardization construction of laboratory digital information
Select " Department Info", the instrument enters department setting window. As shown in figure 6-5.
figure 6-5 Click the scroll bar at the right side of the window, the
instrument can record the serial number continuously.
Click "Back" button, the system exit from the current window and return to blood cell analyzer window.
6.5.1 Add Record Information

Click "Add " button, enters add record window, as shown in figure 6-6. Input the information of
department and abbreviation. Notice:
The coding method of the abbreviation should be easy to memory. In most circumstances, the
abbreviation of the department name is recommended.
Click "Cancel" button, the current input information will be canceled, and the system
- 39 -
returns to department setting window.
Click "OK" button to save the current input information. The system returns to department setting
window.
figure 6-6. 6.5.2 Modify Department Information

Click the number you want to modify. Then press "Modify" button, the system enters modify window,
as shown in figure 6-7.
figure 6-7 Modify the information you want to change. Click
"Cancel" button, the current input information will be canceled, and the system returns to department
setting window.
Click "OK" button to save the current input information, and the system returns to the department set
up windows.
- 40 -
6.5.3 Delete department information

Click the serial number you want to modify, then click "Delete" button, the selected department
information will be deleted.
6.5.4 Print Department Information

Click "Print" button. The instrument will print all the department information saved.
6.6 Operator Information

The operator information can help user setup perfect sorting information in advance, and quicken the
input speed and management of the sample information. Especially the name greatly improves the
communication and standardization construction of laboratory digital information. in the blood cell analyzer
window, press ”menu” button, in the window, Select " setting" in the menu, Select" Doctor Info", the
instrument enters the operator setting window.
figure 6-8 Click the scroll bar at the right side of the window to
display continuously the records of different numbers.
Click "Back" button, the system will exit the current window and return to the main menu window.
6.6.1 Add Operator Information

Click "Add " button. The instrument enters add record window, as shown in figure 6-9.
- 41 -
figure 6-9 Input the information of name and abbreviation. Input
abbreviation information at the left input box of department, the system will select department
automatically. You can also select the department directly from the right input box of the department.
"Status" represents the status of the doctor, including: consigner, operator and inspector. Click the box in
the left directly, blank means no choice, black fork means selected. Selecting more than one item is
allowed. Notice:
The coding method of the abbreviation should be easy to memory. In most circumstances, the
abbreviation of the department name is recommended.
6.6.2 Modify Operator Information

Click the serial number you want to change, then click "Modify" button, the system would enter to
record modify windows, as figure 6-10 shows.
- 42 -
figure 6-10 Modify the information of department and abbreviation
as you want. Click "Cancel" button, the current input information will be canceled, and the system returns
to department setting window. Click "OK" button to save the current input information, and returns the
system to department setting window
6.6.3 Delete operator Information

Click the serial number you want to modify, then click "Delete" button, the selected department
information will be deleted.
6.6.4 Print Operator Information

Click "Print" button. The instrument will print all the operators information saved.
- 43 -
Chapter 7 Review
The instrument can automatically store the results after each sample analysis. The operator can
review, search, modify, print, and delete the data.
7.1 Sample Review

In the blood cell analyzer window, press ”menu” button, in the window, Select " Review" in the menu.
As shown in figure 7-1.
figure 7-1 The data can be reviewed and printed in table. 8 sample
parameters are displayed in each review screen in the order of time sequence. The button of scroll bars
at the bottom of the screen shows the current position. The meaning of the characters as follows: "↑" is
displayed following the parameter indicating that the results exceed the high limit of the system. "↓" is
displayed following the parameter indicating that the results exceed the low limit of the system. "C" is
displayed following the parameter indicating that clogs occur during the test. "B" is displayed following the
parameter indicating that bubbles occur during the test.
➢ Review the sample data Click the left button of scroll bar at the bottom of the screen to review the
rear data. Click the right button of scroll bar at the bottom of the screen to review the front data. Click the
left side of the button at the bottom of the screen to review the data of the next page.
Click the right side of the button at the bottom of the screen to review the data of the front page.
Hold the button at the bottom of the screen, and draw the mouse to review the sample data quickly.
➢ Delete the selected data
- 44 -
The procedures as follows: a) Click "Sample ID" button, the corresponding sample results will be
selected. Click again, the selection will be canceled.
b) Select "Delete" in the menu, pops up a dialog box, let user choose to delete the data or not. Press
"OK" button, the data will be deleted. Press "Cancel" button, the operation will be cancelled.
➢ Delete all data The procedures as follows: a) click "All" button, the data will be selected. Click again,
the selection will be canceled. b) click "Delete" button, pops up a dialog box, and choose to delete the
data or not. Press "OK" button, the data will be deleted. Press "Cancel" button, the operation will be
cancelled.
➢ Print the current sample data in table The procedures as follows: a) Click "All" button at the top of the
screen, the data will be selected. Click again, the selection will be canceled.
b) Click "Print" shortcut key, the selected data would be printed in table..
➢ Search sample data Print the selected data as the following procedures: a) Click "Query" in the menu,
pops up the search window, as shown in figure 7-2. b) Click the button on the left of the item in need to
search. input the key word you want to search.
c) Repeat the b) procedure until the current inputting item finished. Click "Cancel" button to cancel the
searching. Click "OK" button, start to search the data. When the searching finished, the proper sample
data will be displayed on the screen.
figure 7-2 The functions of the menu and shortcut keys as follows:
All: select or recover all the sample data. Detail: Enter the detail review window. Query: Search the
required sample data in any condition.
- 45 -
Delete: Delete the selected data. Print: Print the current data in table. Menu: Exit from the current window.
7.2 Detail Review

In detail review window, select "Detail" by shortcut key. The instrument will access the detail review
window, as shown in figure 7-3. This window review, modify, and print the sample data in graphs.
• Review Click "Next" button to review the next data. Click "Pre" button to review the previous data.
• Modify Sample Information Click "Info" shortcut key, pops up "Information" window. The operation
of this window refers to Chapter 3"Sample Analysis"/ Section3.6.1.
• Modify Sample Data Click "Histogram" button in the menu. The operation procedures as follows:
a) Click "Para" shortcut key, you can select WBC, RBC, PLT in turn. b) Click "Line" shortcut key, you can
select the sorting lines in the histogram. c) Click "Right" or "Left" shortcut key, you can move the sorting
line to right or left. The data calculated according to the new sorting line would be displayed in the data
area at the left side of the screen.
d) Click "Back" shortcut key. If user has modified the sample data, there will a dialog box pops up.
User can select save the modification result or not. Click "OK" button, the result will be saved, Click
"Cancel" button, the result would not be saved and the system exit from histogram modification status.
figure 7-3 The functions of the menu and shortcut keys as
follows: Previous: Review the previous data.
- 46 -
Next: Review the next data. Information: Input and modify the sample data. Print: Print the current sample
data. Back: Exit from the current window and return to the main menu window.
- 47 -
Chapter 8 Service
This chapter gives the description of various service functions provided by the instrument. These
functions will bring user convenient and pleasure. In the blood cell analyzer window, press ”menu” button,
in the window, Select " Service" in the menu.
figure 8-1
8.1 Flush
This function is used for solving general probe clogs. The instrument makes a fixed pressure and
voltage act on the probe to get rid of the clogs.
8.2 Enhanced Flush

This function is use to solve indolent probe clogs. Probe cleaner would be injected into WBC and RBC
count pool. Get rid of the indolent probe clog by soaking it in the probe cleaner. Before performing the
function, user should prepare the probe cleanser well.
8.3 Cleaning
This function is used for the routine cleaning of the probe, sample needle, metering tube
measurement tubing.
8.4 Drain Chambers

This function is used for user to observe the count pool. The instrument will empty the liquid in the
count pool.
8.5 Park
Manufacturer suggests you do not use this function .If the instrument is not going to be used in 2 week or
longer, use this function to clean and empty the instrument. It is convenient
- 48 -
for user to store the instrument. Prepare distilled water before performing the function. .
8.6 Prime Chamber

This function use to Fill diluent to the chamber
8.7 Prime Dilutent

Prime dilutent to relative tubing.
8.8 Prime Lyse

Prime lyse to relative tubing.
8.9 Maintenance
User can finish the weekly maintenance according to the prompt information on the screen. Prepare
probe cleaner before performing the function.
8.10 Mechanic
Enter mechanic window. User can complete the detection of the valve, motor, circuit and interface.
It is helpful to the insurance of the malfunction.
figure 8-2 Valve:
"ON" represents the valve is on. "OFF" represents the valve is off. Motor:
"OK" represents that the motor is in normal status. "FAIL" represents the motor is failure. Recorder:
Click this button, the recorder will print a test paper Printer:
- 49 -
Click this button, the external print will print a test paper Back
It will return to the service menu and the instrument initialize automatically.
figure 8-3
8.11 Special
The instrument enters special maintenance window. User can adjust the running control data.
This function should be performed in guidance of the engineers of authorized by our company
- 50 -
Chapter 9 Maintenance
As other precision instrument, only careful daily service and periodic maintenance can the instrument
have a good working status, and can we get the reliable measurement results and have few malfunctions.
This chapter introduces some preventive methods for service and maintenance. If you want to know
more relative information, please contact the customer service department of our company.
According to the requirements for maintenance of the instrument during the using procedures, we
divide the preventive service and maintenance into the following types: daily, weekly, monthly, yearly and
maintenance according to actual need. Warning:
It is important for the hospital or organization that employs this instrument to carry out a reasonable
maintenance schedule. Neglect of this may result in machine breakdown.
9.1 Routine Maintenance

9.1.1 Clean the Appearance
Wipe the appearance of the instrument with neutral detergent or distilled water. Caution:
Avoid using corrosive acids, alkali, and volatile organic solvent such as: acetone, aether, chloroforms
to wipe the appearance of instrument. Only neutral detergent can be used. Caution: Avoid wiping the
inner of the instrument.
9.1.2 Replace Fuse

The fuse is installed in the fuse-box on the side of power switch. Open the box to replace the fuse
expediently. Appointed specification fuse: F3AL250 Warning:
Only appointed specification fuse can be used.
9.2 Daily Maintenance

It can be divided into two types: run and shutdown.
• Run The instrument has installed daily maintenance procedure, on running ,it can execute auto
cleaning procedure according to the quantity of the sample to keep the instrument in good working status.
Set the auto cleaning procedure according to the section 6.3. The general setting principles are:
Working time > 8 hours, auto-cleaning time=8 hours; 4 hours<Working time<8 hours, auto-cleaning
time=4 hours; Working time< 4 hours, auto-cleaning time=2 hours; The auto-cleaning time will decrease 1
hour year by year.
• Shutdown When the instrument shutdown, it will run daily shutdown auto cleaning procedure. You only
need to clean the workbench and wipe the appearance of instrument when the power-off.
- 51 -
9.3 Weekly Maintenance

This instrument has installed weekly maintenance procedure. Act as follows: a) Prepare probe cleanser.
b) In the main operation window, press "Service” → “Maintenance". Finish all the operations according to
the prompts on the screen.
c) Turn off the power supply. d) Wipe the appearance of instrument as section 9.1.1.
9.4 Monthly Maintenance

It is necessary to clean the dust of the instrument monthly.
Operate as follows: a) Turn off the power supply, and pull out the power line. b) Open the right door and
gently brush the dust of the instrument internal c )After the cleaning, Don't use immediately, run the
cleaning program to rinse the measurement unit automatically.
9.5 Yearly Maintenance

It's necessary to perform preventive maintenance once every year. Because of the highly-requirements
for yearly maintenance, the maintenance should be performed by the authorized engineer of our company.
Please contact the customer service department of our company before yearly maintenance.
9.6 Maintenance before transport or for the instrument that

will not be used for a long time
If the instrument is not going to be used in 2 weeks or longer, or need to pack and transport,
performing the following procedures:
a) In the main operation window, press “Menu” button, select “service
/
Maintenance
“button. b) Operate as screen shows. c) Rotate the bottle covers of rest reagents and store them as the
reagent operation introduction. User should perform efficient action to prevent the material from
deteriorating, mis-eating and misusing. d) Plug the stopples which were pulled out at the first installation
into the corresponding tube connector. e) Pull out diluent connection tube ,, lyse connection tube and
waste connection tube and clean them with distilled water. Dry them in shady place, then packing them
into plastics. f) Pull out the power wire, packing it in plastics after cleaning with neutral detergent. g) Put
the instrument and parts packed in plastics into packing cases.
- 52 -
Chapter 10 Troubleshooting
This chapter contains information that is helpful in identifying and resolving instrument problems that
may occur in the operation of the analyzer. If the problems can not be corrected with the aid of this chapter,
the user should contact the Customer Service Department of our company.
10.1 Abnormal Startup

Methods and procedures:
a) Check if the power is on. b) Check if the power socket is loose. c) Check if the fuse is broken. If
broken, replace it as section 9.1.2.
10.2 Waste Full

Methods and procedures: Dispose of the waste in waste container.
10.3 Diluent Empty

a) Replace diluent. b) In the blood cell analyzer window, Select "Menu/Service/Prime Diluent" in the
menu.
10.4 Lyse Empty

a) Replace lyse. b) In the blood cell analyzer window, Select "Menu/Service/Prime Lyse" in the menu.
10.5 WBC Clog or RBC Clog

If the count time exceeds high limit of the setting during measurement, there will "Clog" alarm occurs.
a) In the blood cell analyzer window, press "Flush" shortcut key. b) If the upper method could not solve
the problem, perform as the following procedures. c) In the blood cell analyzer window, Select "Menu/
Service/ Enhanced Flush" in the menu. Follow the screen prompt information to finish it.
10.6 WBC Bubbles or RBC Bubbles

If the count time exceeds low limit of the setting during measurement, there will be "Bubble" alarm
occurs.
Methods and procedures: In the blood cell analyzer window, Select "MENU/ Service/ Enhanced Flush"
in the menu.
10.7 HGB Error

- 53 -
a) In the blood cell analyzer windows, Select "Menu/Service/ Cleaning". b) If the upper method could not
solve the problem, perform as the following procedures. c) In the blood cell analyzer window, Select
"Menu/ Service/ Enhanced flush" in the menu.
10.8 HGB Bubbles

a) In the blood cell analyzer windows, Select "Menu/Service/ Cleaning". b) If the upper method could not
solve the problem, perform as the following procedures. c) In the blood cell analyzer window, Select
"Menu/ Service/ Enhanced flush" in the menu.
10.9 Recorder out of paper

Methods and procedures: a) Gently press the recorder door to open it. b) Insert the new paper into the
paper entry, and make the printing side towards the thermal head.
c) When the paper juts out from the other side, pull it out and keep it straight. d) Pull the paper out of the
paper exit. e) Close the door of the recorder.
10.10 Recorder too hot

Possible reasons:
The thermal head of the recorder is too hot. Methods and procedures: Suspend using the recorder for 5
minutes.
10.11 The result of background test too high

a) In the blood cell analyzer window, Select "MENU/Service/ Cleaning". b) If the upper method could not
solve the problem, repeat the procedure 3 times. If the problem still can not be solved, perform as the
following procedures.
c) In the blood cell analyzer window, Select "Menu/Service/ Enhanced flush". d) If the upper method could
not solve the problem, repeat the procedure 3 times. If the problem still can not be solved, perform the
upper procedure after replacing all the reagents.
- 54 -
Chapter 11 Help
The instrument provides on-line help during operation. It is helpful for user to solve common problems
and indicate the next operation. Besides, the instrument provides help for user to read and search the
information in window. In the blood cell analyzer window, Select "Menu/Help" in the menu, the help
windows will pop up. As shown in figure 11-1.
figure 11-1 This window shows in chapter mode. User can search
help information conveniently. Click the scroll bar up and down, read the information front and back.
Function of the menu and shortcut keys: Content: Select the required contents.
- 55 -
Appendix 1 Symbol
- 56 -
Biohazard
High Temperature
Caution
Usb Interface
Appendix 2 Specification
2.1 Reagent
Diluent, Lyse,
2.2 Parameter Information

Item Unit
WBC 109 / L
Neu# 109 / L
Lym# 109 / L
Mid# 109 / L
Neu% %
Lym% %
Mid% %
RBC 1012/ L
HGB g/L
HCT %
MCV fL
MCH pg
MCHC g/L
RDW-SD fL
RDW-CV %
PLT 109 / L
MPV fL
PDW None
PCT %
p-LCR %
RBC Histogram None
PLT Histogram None
WBC Histogram None
2- 1 Parameter Information
2.3 Sampling characteristics

Test Mode Sample Volume Venous mode 10 μL Peripheral mode 10 μL Prediluent Mode 20 μL
2- 2 Sampling characteristics
2.4 Performance index

Item Range
- 57 -
WBC 0~200.0×109/L
RBC 0~20.00×1012/L
HGB 0~300g/L
PLT 0~2000×109/L
HCT 0%~80%
2- 3 Performance index
2.5 Background Range

Item Range
WBC
≤ 0.2 × 109 / L
RBC
≤ 0.02× 1012/ L
HGB ≤ 1 g / L
HCT ≤ 0.5 %
PLT
≤ 10 × 109/ L
2- 4Background Range
2.6 Linear Range

Item Range SD
WBC
0~5.9×109/L 6.0~99.9×109/L
- 58 -
(±0.3×109)/L ±5%
RBC
0~0.99×1012/L 1.0~9.99×1012/L
(±0.05×1012)/L ±5%
HGB
0~99g/L 100~300g/L
±2.0 g/L ±2%
PLT
0~99×109/L 100~999×109/L
(±8.0×109)/L ±10%
2- 5 Linear Range
2.7 Repeatability index

Test the sample for 11 times ,then use the results from the second to eleventh test to get repeatability
statistics.
Item CV Range
WBC ≤2.0% (4.0~15.0×109)/L
RBC ≤1.5% (3.5~6.0×1012)/L
HGB ≤1.5% (110.0~180.0g)/L
MCV ≤0.4% (80.0~110.0)fL
PLT ≤4.0% (100.0~500.0×109)/L
LYM% ≤8.0%
LYM%≥15.0%, WBC≥4.0×109/L
Item CV Range
MID% ≤10.0%
MID%≥5.0%, WBC≥4.0×109/L
NEUT% ≤8.0%
NEUT%≥30.0%, WBC≥4.0×109/L
2- 6 Repeatability index
2.8 Pollution rate

Item Result
WBC ≤1%
RBC ≤1%
HGB ≤1%
PLT ≤1.5%
2- 7 Pollution rate
2.9 Input/Output
Touch Pen Keyboard(Option) Mouse(Option) Printer(Option) R232 Port VGA Port Internet Port Power
supply:
Voltage Frequency Main Unit 100~240Vac 50/60 Hz Work environment
Temperature: 18°C~35°C Humidity: 10%RH ~90%RH Atmospheric Pressure: 70.0kPa ~106.0kPa
Storage environment Temperature: -10°C~40°C Humidity: 10%RH ~90%RH Atmospheric Pressure:
50.0kPa ~106.0kPa This instrument should be placed at a good ventilation area. Dimensions and Weight
Dimensions: 410mm×435mm×472mm Weight: 25kg Contraindication : None
- 59 -

Genius 3diff Operator Manual

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Vec 3 Diff Operation Manual Edition: A/0

Chapter 1 Instrument Introduction ................................................................................... 6 1.1 Name

How to use the manual

Avoidance from potential hazard

Chapter 1 Instrument Introduction

1.6.1 Display Screen

1.7 Detection Principle

1.7.2 Principles of HGB Measurement

1.7.3 Volume Distribution of Blood Cell

2.3 Installation Requirements

2.3.2 Space Requirement

2.4 Reagent tubing connection

2.4.1 Lyse Connection

2.4.2 Diluent Connection

2.4.3 Waste Connection

2.5 Recorder Paper Installation

2.6 Keyboard and mouse installation

2.7 Printer Installation (Optional)

2.8 Power Cable Connection

Chapter 3 Sample Analysis

3.3 Background Test

3.4 Quality Control

3.5 Preparation for sample collection

3.5.1 Venous Blood Collection

3.5.2 Capillary Blood Collection

3.5.3 Prepare Samples in Prediluted Mode

3.5.4 Prepare Samples in Capillary Mode

3.5.5 Sample Homogenization

3.6 Sample count and Analysis

3.6.2 Procedures of Sample Count and Analysis

3.7 Analytical Results Modification

Chapter 4 Quality Control

4.1 Edit Quality Control Files

4.2 Quality Control Run

4.3 Quality Control Graph

4.4 Quality Control Table

5.1 Operator calibration

5.1.4 Modify the Calibration Coefficient

5.1.3 Calculate Calibration Factors

5.2 Auto Calibration

6.1 Date & Time

6.5 Department information setting

6.5.1 Add Record Information

figure 6-6. 6.5.2 Modify Department Information

6.5.3 Delete department information

6.5.4 Print Department Information

6.6 Operator Information

6.6.1 Add Operator Information

6.6.2 Modify Operator Information

6.6.3 Delete operator Information

6.6.4 Print Operator Information

7.1 Sample Review

7.2 Detail Review

8.2 Enhanced Flush

8.4 Drain Chambers

8.6 Prime Chamber

8.7 Prime Dilutent

8.8 Prime Lyse

9.1 Routine Maintenance