Konelab
Konelab
Konelab
Code: 895304
Manual version: L
Date: 12.5.2008
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II LAS INTERFACE
Copyright
Copyright 2008 Thermo Fisher Scientific Inc. All rights reserved.
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1. HARDWARE INTERFACE
Any reference to the The Konelab LIMS hardware interface works through serial communication channel.
Konelab systems also
refers to the T Series.
Figure 1: The connectors for serial communication channels are at the back of the
Konelab workstation. The connectors are 9-pin male D-connectors.
Main window
F8/F1
Configuration
Configuration
F7
LIMS /LAS
Configuration
LIMS/LAS Configuration
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The cable should be The signals needed at the Konelab end of cable
made according to the
documentation of LIMS Pin 2 Receive Data
system in use. Pin 3 Transmit Data
Pin 5 Ground
This example describes An example of the cabling between Konelab and LIMS
the minimum cable
connections needed. Konelab and a PC (RS-232)
Some computer systems
may require some Konelab PC
additional signals
connected locally within Pin 2 RxD--------------------------- TxD
connector. Please refer Pin 3 TxD ----------------------------- RxD
to your LIMS
documentation for Pin 5 Gnd ----------------------------- Gnd
further information.
1.1.1 INSTALLATION
The ASTM software can be taken into use after the Konelab software is installed. If
Konelab application is running, close the application by selecting Management and
Exit. Select Start->Programs->Konelab Lims Selection and select ASTM serial or
ASTM TCP/IP from there. The ASTM configurations should be checked after
starting the Konelab application again, because the configuration file is updated in
start of the application.
If ASTM communication will use serial line, selection should be “ASTM serial”. If
TCP/IP communication is used, “ASTM TCP/IP” should be selected. TCP/IP
communication needs configuration described in the chapter 1.1.3 before it can be
used.
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1.1.2 CONFIGURATION
ASTM software has additional configurations as compared to Konelab Online
software. The configuration file is Konelab.ini and it is in folder C:\Konelab\Config.
LimsProtocol = ASTM
- This is a new choice for protocol.
SentryWaitsLimsRequests = YES
- When YES the sample entry function waits for the response to a query when new
sample is introduced.
ASTMControlResultSending = NO
- When YES the new control sample analysis results are automatically send to
Laboratory computer.
ASTMStandardResultSending = NO
- When YES the new calibrator sample analysis results are automatically send to
Laboratory computer.
SampleIdSendingDelay = 0
- If value is not 0, Konelab will use this delay between successive sending of new
sample Id's. This can be used to ease the burden on Laboratory computer when
e.g. a full segment with 14 samples is introduced. The value is expressed in
milliseconds.
ResultSendingDelay = 0
- If value is not 0, Konelab will use this delay between successive sending of new
sample results. This can be used to ease the burden on Laboratory computer. The
value is expressed in milliseconds.
In order to take ASTM protocol in use the configuration file should be edited
manually. Close the Konelab application by selecting MANAGEMENT and EXIT
from there. Wait for the Konelab application to shut down. Start Windows NT
Explorer by selecting Start-> Programs->Windows NT Explorer. Select the folder
C:\Konelab\Config and on the right side of Explorer display should be seen files
Konelab and UserText. Double click with mouse on Konelab file. Now the Notepad
program should open with the Konelab.ini file. Change the configuration manually
and after that save the modified file selecting File->Save. Now the Konelab software
can be started again with ASTM configurations.
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Note! If 7 data bits is - Baud rate Select the baud rate values between 110 and 9600.
selected special - Data bits The number of data bits can be 7 or 8.
characters above
ASCII 127 are not sent - Stop bits The number of stop bits can be 1 or 2.
correctly. - Parity Select the parity checking. It can be even or odd.
If checking is not wanted select NO.
- Ack timeout (sec) The maximum time the response is waited for.
Socket Communication
Konelab’s socket communication is based on Windows Sockets and it uses TCP/IP
connection oriented sockets. This means that a connection is needed between the
two parts willing to communicate before any information transmission can occur.
When there is no more need to communicate the connection can be disconnected,
but is not needed until closing the session, clearing daily files or in an error
condition.
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Communication protocol
Konelab uses ASTM protocol in socket communication. This means that the serial
channel is simply replaced with socket communication and all the interactions and
message structures are the same as when using a serial channel. Please refer to
chapter 3 for details in Konelab implementation of ASTM protocol.
Configuration
The new card should be configured to use the specific TCP/IP address that is
reserved for the communication and this should be done with Windows Control
Panel’s Network tool or ‘Network Connections’-folder. Besides this the setting of
routes is needed. If the address for the new card is 172.16.0.11 and the other end for
communication is 172.16.0.XXX, then the following should be done. In command
line window give the following commands:
These commands make permanent entries to route table, so Windows Sockets can
tell which card to use for communicating with certain IP-address. If the address of
the new card is not 172.16.0.11, the last command line should be changed to match
the address.
Main window
F8/F1
Configuration
Configuration
F7
LIMS/LAS
Configuration
LIMS/LAS
Configuration
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Besides the card configuration, the connection parameters for socket communication
must be also set. The TCP/IP connection is selected by choosing value TCP/IP to
communication type.
Konelab address:
- This is IP-address configured to new network card
Lims address:
- This is IP-address for LIS/LIMS system
Lims port:
- This defines communication port which is opened to ASTM communication
For successful use of communication both ends must use same port number and
matching IP addresses.
Connection management
Konelab tries to get connected whenever possible. In client mode Konelab tries to
create a connection once in every 20 milliseconds. In server mode Konelab is
waiting for a connection. After the connection is established, Konelab does not
disconnect until application is closed down, clear daily files is requested by user or
an unrecoverable error condition occurs in communication.
Clearing daily files also disconnects Konelab because the internal message queues
are cleared. Konelab then waits for a few seconds before accepting any new data
through LIS connection. This is to ensure that Konelab is internally in consistent
state before starting a new session. There is no error message to user when
disconnecting is done due to clear daily files.
If communication error occurs, Konelab disconnects and shows the user an error
message: 4403 Write error (LIMS), 4404 Read error (LIMS), 4406 Communication
timeout (LIMS) or 4407 Transmit error (LIMS). This indicates to the user about
problems in communication. Konelab tries, however, to connect immediately after
the disconnecting has occurred. The blue Online signal on Konelab screen indicates
a communication trial is going on, but does not indicate a successful communication.
Debugging sockets
The debug support for sockets is practically the same as with serial channel, but
setting C:\Konelab\Config\Konelab.ini DebugLevel to value 1 will provide a more
detailed information of data transmission through socket. This should help to detect
problems when creating the first implementation of communication. After the
connection seems to work the DebugLevel should be returned to value 9 in order to
keep the LSDEBUG.TXT file a bit smaller.
When debug is on and some fault has occurred, Konelab shows a yellow or red
Online signal on screen, when no transmission is active. This replaces the normal
gray background and is an indication to user that some fault has occurred. The
reason for the fault can be found from LSDEBUG.TXT with search to words ‘RED’
or ‘YELLOW’. Note that this works only when debugging is on.
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) Function 1 is used when Patient data, Sample data and Test data is sent to
the analyser.
) Function 2 is used to send Sample and Test data only to the analyser.
Control and Calibrator sample data can also be sent to the analyser using this
function.
These functions are activated using the initialisation records. Refer to section 2.1.1.1.
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Initialisation records are activating commands which the host computer sends to the
analyser when the communication begins.
INITIALISATION RECORD 1
This record is used when Patient data, Sample data and Test data is sent to the
analyser.
:I:PR1/C/
INITIALISATION RECORD 2
This record is used to send Sample and Test data only to the analyser. Control and
Calibrator sample data can also be sent to the analyser using this initialisation record.
:I:PR2/C/
INITIALISATION RECORD 3
This record is used when a laboratory computer wishes to receive all newly
completed results which have not already been sent.
:I:PR3/C/
INITIALISATION RECORD 4
This record is used when a laboratory computer wishes to receive all results
completed that day.
:I:PR4/C/
INITIALISATION RECORD 5
This record is used when a laboratory computer wishes to receive all results for a
particular sample. Complete and incomplete tests are sent by the analyser.
:I:PR5/C/
INITIALISATION RECORD 6
This record is used when a laboratory computer wishes to know which patient
samples exist on the database of the analyser. The laboratory computer can then send
new requests.
:I:PR6/C/
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INITIALISATION RECORD 7
This record is used when a laboratory computer wishes to know which tests exist on
the database of the analyser.
:I:PR7/C/
The slash (/) must be entered into the string, since it is the field delimiter. This
means that the number of slashes in a record is always constant. Note that the slash is
a forbidden character inside a field.
For example, if no sender information and no age information is sent, the Data String
looks like:
:P:nn..n/ii..i/M/A///n or o/C/
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:S:ii..i/pn/pp/st/nn/dd..d/tt..t/rrr/n or o/C/
The STAT identifier indicates that the following request must be performed urgently.
This is valid only for one request following the STAT identifier. If there is a STAT
profile to be sent, each test identification must have its separate STAT identifier. If
the '!' is omitted then the request is performed routinely,
i.e. :A:xx..x/yy..y/.../C/
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:R:ii..i/aa..a/rr..r/ddd/ee..e/aa..a/rr..r///.../C/
If the result was obtained without a dilution, the dilution field is skipped (/ remains).
If the result has no error, the error field is skipped, (/ remains).
Finishing record is used in data sending as the last record to indicate to receiver that
communication is over. The receiver must always acknowledge finishing record by
<ACK>.
:F:PR/C/
where C is the check sum. Refer to section 2.1.3.1 for the calculation of the check
sum.
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(ACK)
(ACK)
(ACK)
(ACK)
(ACK)
(ACK)
(ACK)
(ACK)
(ACK)
(Finishing record)(CR)(LF)
(ACK)
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(ACK)
(ACK)
(ACK)
(ACK)
(ACK)
(Finishing record)(CR)(LF)
(ACK)
(ACK)
(Result record)*(CR)(LF)
(ACK)
(Finishing record)(CR)(LF)
(ACK)
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(ACK)
(Result record)*(CR)(LF)
(ACK)
(Finishing record)(CR)(LF)
(ACK)
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(ACK)
(ACK)
(Result record)*(CR)(LF)
(ACK)
(Finishing record)(CR)(LF)
(ACK)
***
(ACK)
(Result record)*(CR)(LF)
(ACK)
(Finishing record)(CR)(LF)
(ACK)
***
(Finishing record)(CR)(LF)
(ACK)
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**
(Test analysis record)(CR)(LF)
(ACK)
(Finishing record)(CR)(LF)
(ACK)
==2==
(Finishing record)(CR)(LF)
(ACK)
(ACK)
(Finishing record)(CR)(LF)
(ACK)
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Each record begins with a colon ':' and ends with a check character 'C' followed by a
slash '/', carriage return and line feed.
The check sum is calculated from the colon to the last slash before the check sum
character.
: I : P R 2 / @ /
The time the analyser needs for validation of a record depends on the situation and in
the worst case it may take few minutes. Recommended time out value is at least 3
minutes when waiting the response from the analyser.
When the host computer asks results from the analyser with certain intervals, the
recommended minimum time between consecutive asking is at least 5 minutes.
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:I:PR1/?/
ACK
:P:SMITH JOHN/4///30/2/N/8/
ACK
:S:6///PL/HA/97-12-18/12:13/0/N/7/
ACK
:A:ISE/ALB/ALK.PH/ALT/DBIL/GGT/PROT/T/
ACK
:P:JONES SUSAN/23423///97-12-08/3/O/_/
ACK
:S:12/2/1/SE//97-12-07/09:45/0/O/)/
ACK
:A:!/ALB/!/ALK.PH/!/ALT/!/DBIL/!/GGT/@/
ACK
:F:PR/+/
ACK
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:A:ISE/ALB/ALK.PH/ALT/DBIL/GGT/PROT/T/
ACK
:S:12/2/1/SE//97-12-07/09:45/0/O/)/
ACK
:A:!/ALB/!/ALK.PH/!/ALT/!/DBIL/!/GGT/@/
ACK
:F:PR/+/
ACK
ACK
ACK
ACK
:F:PR/+/
ACK
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ACK
ACK
ACK
:F:PR/+/
ACK
:S:6/// PL/HA/97-12-18/12:13/0/N/7/
ACK
ACK
:F:PR/+/
ACK
:F:PR/+/
ACK
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:I:PR6/D/
ACK
ACK
:A:GLUC/CREA/CA/ALB/CHOL/_/
ACK
:F:PR/+/
ACK
:A:CA/ALB/CHOL/;/
ACK
:F:PR/+/
ACK
:F:PR/+/
ACK
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:I:PR7/E/
ACK
ACK
ACK
:F:PR/+/
ACK
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For example:
(ACK)
(ACK)
(ACK)
(ACK)
(Finishing record)(CR)(LF)
(ACK)
When the laboratory computer receives an error record, the analyser expects a
finishing record to be sent back to acknowledge the error situation. The analyser
acknowledges this by sending back an ACK. After this, the protocol begins again
with the laboratory computer starting the bidding with the initialisation record. All
data up to the ACK sent by the analyser prior to the error is saved in the analyser,
including also the requests at the beginning of an analysis record up to the wrong test
identification.
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002; result rejected, result will never come, e.g. one ISE electrode
was disabled
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)
E.g. electronic malfunction, software error,
Possible causes initialisation error or power failure.
Check the cable and cable connection. If the problem
persists, print the reports and call service.
)
E.g. electronic malfunction, software error,
Possible causes initialisation error or power failure.
Check the cable and cable connection. If the problem
persists, print the reports and call service.
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)
E.g. faulty cable, electronic malfunction, software
Possible causes error.
Check the cable and cable connection. If the problem
persists, print the reports and call service.
1) A data record is a string of any characters beginning with ':' and ending with
(0D hex) or a string of any characters whose length exceeds the size of input buffer
(currently 132).
)
E.g. faulty cable, electronic malfunction or wrong
Possible causes initialisation data.
Check the cable and cable connection. If the problem
persists, print the reports and call service.
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:I:PR2/@/
ACK
:E:105;XXX /A/
ACK
:F:PR/+/
ACK
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2) Samples
First introduce samples with the right sample types, after that give the test request for
the calculated test.
The right sample type here means the sample type belonging to the calculated test
request.
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:F:PR/+/
:I:PR2/@/
:S:Sample3///S//////6/
:A:LDL/@/
:F:PR/+/
Reporting
Reporting gives result of calculated (patient) test according to a patient. In addition
the external test is reported according to a patient. Results of tests including into the
calculated test are reported separately according to samples.
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3. ASTM PROTOCOL
The ASTM Laboratory Information Management System interface is based on the
Any reference to the following standards: CLSI LIS2-A "Standard Specification for Transferring
Konelab systems also Information Between Clinical Instruments and Computer Systems" and CLSI LIS1-A
refers to the T Series. "Standard Specification for Low-Level Protocol to Transfer Messages Between
Clinical Laboratory Instruments and Computer Systems”.
CLSI LIS1-A was formerly defined by ASTM in standard 1381-95 and CLSI LIS2-A
was formerly defined by ASTM in standard 1394-91.
3.1 FEATURES
The physical transmission layer is implemented according to CLSI LIS1. The cable
connector is a 9-pin male connector instead of the standard 25-pin male connector.
The physical transmission layer using TCP/IP connection is described in the section
1.1.3.
The logical layer contains selected portions of CLSI LIS2. The detailed record
structure is described in section 3.2.1 ASTM in Konelab: CLSI LIS2-A Structure of
messages.
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Patient weight 18 - -
Patient’s known 19 - -
or suspected
diagnosis
Patient active 20 - -
medications
Patient’s diet 21 - -
Practice field 1 22 - -
Practice field 2 23 - -
Admission or 24 - -
discharge dates
Admission 25 - -
status
Sender 26 (X) (X)
Native of 27 - -
alternative
diagnostic code
and classifiers
Alternative 28 - -
diagnostic code
and
classification
Patient religion 29 - -
Marital status 30 - -
Isolation status 31 - -
Language 32 - -
Hospital 33 - -
service
Hospital 34 - -
institution
Dosage 35 - -
category
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Date of change 10 - -
in instrument
normative
values or units
Operator 11 - (X) User login name if User levels have been
identification set on
Date/time test 12 - -
started
Date/time test 13 (X) (X) Form YYYYMMDDHHMMSS. No
completed value if test is not completed.
Instrument 14 - X Instrument ID is defined in konelab.ini in
identification parameter AnalyzerName.
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X X results)
D –requesting demographics only
- - Note: not repeated.
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Note the reference 6) Analysing the sample is completed. Konelab sends the
class 'HH'-flag in R- results:
record.
Konelab:<enq>
Host: <ack>
Konelab:<stx>1H|\^&|||60^1^5.0|||||||P||20010502124833<cr><etx>9D<cr><lf>
Host: <ack>
Konelab:<stx>2P|1|123456-789A|||LastName
FirstName|||Adult|||||||||||||||||||||||||<cr><etx>C2<cr><lf>
Host: <ack>
Konelab:<stx>3O|1|S1234^3.0^1^2||^^^ALL^|R||||||X||||1|||||||||1|F<cr><etx>AD<cr><lf
>
Host: <ack>
Konelab:<stx>4R|1|^^^Ca^3.0|9.2|mmol/l|2.2^2.7^Adult|HH||F||||20010502124835|1
<cr><etx>50<cr><lf>
Host: <ack>
Konelab:<stx>5L|1|N<cr><etx>08<cr><lf>
Host: <ack>
Konelab:<eot>
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Signal Pin
RxD (received data) 2
TxD (transmitted data) 3
GND (ground) 5
Communication parameters are 9600 baud, 8 bit, 1 start bit, 1 stop bit, no parity. No
hardware or software flow control is used.
STX 0x02
ETX 0x03
ACK 0x06
NACK 0x15
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Each communication record contains also field for message length. Message length
doesn’t include STX and ETX characters.
ucChecksum = 0;
for (iIndex = 0; iIndex < iNumOfChars; iIndex++)
{
ucTemp = pString[iIndex];
ucChecksum = ucChecksum + ucTemp;
}
return ucChecksum;
}
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After that LAS sends Analyser status query record to the analyser. Analyser waits
for this record up to 10 seconds. If the record is received in time, analyser responses
with ACK and sends Analyser status record to LAS. LAS sends ACK to the analyser
to finish the initialisation sequence.
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When ‘busy’ response is received from Konelab either as response to status query or
to aspirate sample –command, Konelab is either initializing or recovering from an
error situation. This is a temporary state and LAS should start polling with status
query (or aspirate sample –command) until status value other than ‘busy’ is received.
Typically this will take 10-20 seconds and shouldn’t take more than a minute. Until
then sample tubes must not be moved as KUSTI dispenser may be extended at the
LAS position. If status value ‘ready’ is received, the recovery was successful and
another aspirate sample –command may be sent. If the recovery was unsuccessful,
status ‘out of order’ or ‘not in use’ is returned.
Konelab does not automatically try to recover from states ‘out of order’ or ‘not in
use’.
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The diagram below explains the possible states and transitions of the Konelab LAS
interface and when LAS is allowed to move sample tubes.
2. Analyser is busy
LAS status : moving not allowed
analyzer status: ok
ANALYSER STATUS
3. Fatal error when aspiration RECORD,
LAS status : moving not allowed status=1(busy)
analyzer status: unknown received
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LAS ANALYSER
Initialisation record
5 s timeout
Initialisation record
ACK
Max 10 s
Status query record
ACK
Status Record
ACK
LAS ANALYSER
ACK
ACK
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ACK
wait 10 s
Status query record
ACK
status record
status = BUSY
ACK
wait 10 s
Status query record
ACK
status record
status = BUSY
ACK
wait 10 s
Status query record
ACK
status record
status = READY
ACK
ACK
09/05/2008
Konelab
LIMS and LAS Interfaces 895304 55
LIMS AND LAS INTERFACES
ACK
wait 10 s
Aspirate sample record
ACK
ACK
wait 10 s
Aspirate sample record
ACK
ACK
wait 10 s
Aspirate sample record
ACK
ACK
09/05/2008
Konelab
LIMS and LAS Interfaces 895304 56
LIMS AND LAS INTERFACES
ACK
wait 10 s
Status query record
ACK
Status record
Status = BUSY
ACK
wait 10 s
Status query record
ACK
Status record
Status = BUSY
ACK
wait 10 s
Status query record
ACK
Status record
Status = NOT IN USE or
Status = OUT OF ORDER
ACK
09/05/2008
Konelab
LIMS and LAS Interfaces 895304 57