Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Scribd Logo
Upload

Welcome to Scribd!

  • 425 views

    Micrografting

    Uploaded by

    DrRuby Ranjan Sharma
    Micrografting involves grafting meristematic tissues from one plant onto another in vitro to combine their characteristics. It was first used in 1952 on vegetables, with success rates as high as 80-90% for tomatoes. Micrografting involves placing a maintained scion onto an in vitro grown rootstock under aseptic conditions. The results can then be multiplied in tissue culture or acclimatized outdoors. It combines the advantages of rapid in vitro multiplication with increased productivity from superior rootstock-scion combinations.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Micrografting

    Uploaded by

    DrRuby Ranjan Sharma
    425 views1 page
    Micrografting involves grafting meristematic tissues from one plant onto another in vitro to combine their characteristics. It was first used in 1952 on vegetables, with success rates as high as 80-90% for tomatoes. Micrografting involves placing a maintained scion onto an in vitro grown rootstock under aseptic conditions. The results can then be multiplied in tissue culture or acclimatized outdoors. It combines the advantages of rapid in vitro multiplication with increased productivity from superior rootstock-scion combinations.

    Original Description:

    micrografting

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Micrografting involves grafting meristematic tissues from one plant onto another in vitro to combine their characteristics. It was first used in 1952 on vegetables, with success rates as high as 80-90% for tomatoes. Micrografting involves placing a maintained scion onto an in vitro grown rootstock under aseptic conditions. The results can then be multiplied in tissue culture or acclimatized outdoors. It combines the advantages of rapid in vitro multiplication with increased productivity from superior rootstock-scion combinations.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    425 views1 page

    Micrografting

    Uploaded by

    DrRuby Ranjan Sharma
    Micrografting involves grafting meristematic tissues from one plant onto another in vitro to combine their characteristics. It was first used in 1952 on vegetables, with success rates as high as 80-90% for tomatoes. Micrografting involves placing a maintained scion onto an in vitro grown rootstock under aseptic conditions. The results can then be multiplied in tissue culture or acclimatized outdoors. It combines the advantages of rapid in vitro multiplication with increased productivity from superior rootstock-scion combinations.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 1

    Micrografting (shoot tip grafting)

    • The term micro grafting means the grafting of meristamatic tissues in vitro in the laboratory in
    which the shoots of one plant are grafted on to the shoots or roots of another plant so as to
    combine the characteristics of the two plants.
    This method was used for the first time in 1952 by Morel and Martin . Micrografting was
    initially used on vegetables. The success rate of micrografting is relatively high (80– 90%) for
    tomatoes.
    Micrografting consists of the placement in aseptic conditions of a maintained scion onto an in
    vitro grown rootstock.
    • The results of in vitro micrografting and the plant material derived from it can be further
    multiplied in tissue culture conditions or acclimatized to outdoor conditions.
    • Micrografting is a technique that potentially can combine the advantages of rapid in vitro
    multiplication with increased productivity that results from grafting, superior rootstock and scion
    combinations
    . • Among various methods of micrografting, slit or wedge grafting has been found most suitable
    in case of fruit crops.
    • In vitro shoot tips are better as compared to in vivo shoot tips for carrying out micrografting
    resulting in higher graft success, less contamination, lower shoot tip necrosis and good vigour of
    micrografts.
    This comprises the following steps
    • Aseptic condition was maintained throughout all stages of micrografting process.
    • Seedlings were removed carefully from the agar medium with sterilized forceps.
    • For use as rootstock, seedlings were cut back to 1 cm above the cotyledonary nodes and leaves
    were excised using a microscalpel.
    • Terminal shoots to be used as scions were excised from the other seedlings at 2 cm below the
    apex. • Lower leaves removed from the basal 1 cm of scion.
    • Excised scion and rootstocks were stored in sterile de-ionized water until used in the grafting
    process.
    The shoot tip was inserted at the top of decapitated rootstock by making an incision.
    • The cortex was exposed by the horizontal cut of the incision.
    • Grafted plants were cultured in a liquid nutrient medium containing plant cell culture salt
    solution of MS, modified White's vitamins and 75 g/1 sucrose.
    • When at least two expanded leaves were formed micrografted plants were transferred to pots
    containing a steam sterilized soil mixture suitable for plant.
    Importance
    • Overcoming graft incompatibility
    • Rapid mass propagation of elite scions by grafting onto rootstocks that have desirable traits like
    resistance to soil borne pathogens and diseases
    • To allow survival of difficult to root /shoots
    • Development of virus free plant

    You might also like