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    Calibration of A Pipette

    Uploaded by

    Lisa Sawh
    1. The document describes a procedure to calibrate a pipette by measuring the mass of water delivered in repeated trials to determine its accuracy and precision. 2. The procedure involves weighing water delivered from the pipette into a beaker over four trials, calculating the mean volume delivered, and using that to find the standard deviation as a measure of precision. 3. The results would show the mean volume delivered by the pipette along with the standard deviation to express the accuracy and precision based on how close the measured volumes are to the nominal volume.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Calibration of A Pipette

    Uploaded by

    Lisa Sawh
    33 views1 page
    1. The document describes a procedure to calibrate a pipette by measuring the mass of water delivered in repeated trials to determine its accuracy and precision. 2. The procedure involves weighing water delivered from the pipette into a beaker over four trials, calculating the mean volume delivered, and using that to find the standard deviation as a measure of precision. 3. The results would show the mean volume delivered by the pipette along with the standard deviation to express the accuracy and precision based on how close the measured volumes are to the nominal volume.

    Original Title

    CALIBRATION OF A PIPETTE

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    1. The document describes a procedure to calibrate a pipette by measuring the mass of water delivered in repeated trials to determine its accuracy and precision. 2. The procedure involves weighing water delivered from the pipette into a beaker over four trials, calculating the mean volume delivered, and using that to find the standard deviation as a measure of precision. 3. The results would show the mean volume delivered by the pipette along with the standard deviation to express the accuracy and precision based on how close the measured volumes are to the nominal volume.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    33 views1 page

    Calibration of A Pipette

    Uploaded by

    Lisa Sawh
    1. The document describes a procedure to calibrate a pipette by measuring the mass of water delivered in repeated trials to determine its accuracy and precision. 2. The procedure involves weighing water delivered from the pipette into a beaker over four trials, calculating the mean volume delivered, and using that to find the standard deviation as a measure of precision. 3. The results would show the mean volume delivered by the pipette along with the standard deviation to express the accuracy and precision based on how close the measured volumes are to the nominal volume.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    CALIBRATION OF A PIPETTE [Procedure is the same for burette]

    Introduction
    An ordinary laboratory pipette may be expected to deliver its nominal volume with good precision and
    good accuracy if it is used in the way recommended. In this experiment we investigate the precision and
    accuracy of such a pipette by making accurate determinations of the mass of water it delivers in repeated
    operations.

    Materials
    25 cm3 pipette, 50-cm3 beaker, thermometer, pipette filler, graph paper.

    Procedure (Read carefully)

    1. Clean the beaker and the pipette and dry the beaker.
    2. Obtain distilled water in a conical flask and let it stand on the bench for about 15 min before
    determining its temperature.
    3. Weigh the beaker on a balance which allows you to determine the mass to the nearest tenth of a
    milligram (i.e. ± 0.001 g).
    4. Fill (use the pipette filler) and discharge the pipette as recommended, into the beaker, and determine
    the mass of water discharged by taking the difference between mass of beaker and mass of beaker
    and water.
    5. Repeat step 4 until you have the results of four (4) such trials.

    Calculations
    1. Find the mean of the four trials. The mean volume Vo can now be determined from the mean mass and
    the density of water at the temperature you determined in step 2 above.

    2. Find the mean volume of water discharged by the pipette using the formula:

    Mean Volume: Vo = mean mass / density of water

    3. Comment on the accuracy of the pipette.

    4. We now want to determine the precision of the pipette.

    Table of Mass and Volume of Water obtained in 4 Pipette Trials

    Trial No. Mass of water/g Vol. of water, Vi/cm3 (Vi – Vo) = di (Vi - V)2 = di2

    The standard deviation, σ, is given by the formula:

    s = √ {∑(Vi – Vo)2 / (N-1)}


    Write your result for the mean volume you determined in step 2 with the error(standard deviation you have
    now estimated from the data;
    Volume of pipette = Vo (± s)

    Comment now, on the accuracy and precision of the pipette.

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