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    Preparation of Solutions and Buffers

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    toby the dog
    This experiment aimed to teach students how to prepare solutions and buffers. It described making various solutions like 10% NaCl, 4% NaCl, 80% ethanol, and 1.2M NaCl. It also described preparing buffers like 0.1M Tris-HCl at pH 7 and pH 4, 0.3M Tris-HCl at pH 7, and 0.4M Tris-HCl at pH 7. Tris, or tris(hydroxymethyl)aminomethane, is a common buffering agent used in the pH range of 7-9. The document emphasized accurately labeling solutions and explained how to use concentration formulas to calculate amounts of solutes and solvents needed. The overall goal was

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    Preparation of Solutions and Buffers

    Uploaded by

    toby the dog
    80 views9 pages
    This experiment aimed to teach students how to prepare solutions and buffers. It described making various solutions like 10% NaCl, 4% NaCl, 80% ethanol, and 1.2M NaCl. It also described preparing buffers like 0.1M Tris-HCl at pH 7 and pH 4, 0.3M Tris-HCl at pH 7, and 0.4M Tris-HCl at pH 7. Tris, or tris(hydroxymethyl)aminomethane, is a common buffering agent used in the pH range of 7-9. The document emphasized accurately labeling solutions and explained how to use concentration formulas to calculate amounts of solutes and solvents needed. The overall goal was

    Original Description:

    Short report the preparation of solutions and buffers

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    This experiment aimed to teach students how to prepare solutions and buffers. It described making various solutions like 10% NaCl, 4% NaCl, 80% ethanol, and 1.2M NaCl. It also described preparing buffers like 0.1M Tris-HCl at pH 7 and pH 4, 0.3M Tris-HCl at pH 7, and 0.4M Tris-HCl at pH 7. Tris, or tris(hydroxymethyl)aminomethane, is a common buffering agent used in the pH range of 7-9. The document emphasized accurately labeling solutions and explained how to use concentration formulas to calculate amounts of solutes and solvents needed. The overall goal was

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    80 views9 pages

    Preparation of Solutions and Buffers

    Uploaded by

    toby the dog
    This experiment aimed to teach students how to prepare solutions and buffers. It described making various solutions like 10% NaCl, 4% NaCl, 80% ethanol, and 1.2M NaCl. It also described preparing buffers like 0.1M Tris-HCl at pH 7 and pH 4, 0.3M Tris-HCl at pH 7, and 0.4M Tris-HCl at pH 7. Tris, or tris(hydroxymethyl)aminomethane, is a common buffering agent used in the pH range of 7-9. The document emphasized accurately labeling solutions and explained how to use concentration formulas to calculate amounts of solutes and solvents needed. The overall goal was

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 9

    Experiment 3: Preparation of Solutions and Buffers

    Objectives:

    ● To learn to prepare solutions and buffers

    ● To understand the basic concepts of preparing solutions and buffers

    Materials

    • Volumetric flasks/conical flasks/beakers

    • Blue capped Schott bottles

    • Glass pipettes, bulbs or pumps

    • Pipettes and tips

    • Distilled water

    • Sodium hydroxide (NaOH)

    • Sodium chloride (NaCl)

    • Absolute ethanol (95%)

    • 12 M Hydrochloride acid (HCl)

    • Tris

    Methods
    (A) Preparation of Solutions

    1. Weigh 10 g NaCl is weighed and dissolved in 100 g distilled water to prepare for 10%

    (w/w) of NaCl in distilled water. [Prepare for 5 mL]

    2. 4 g of NaCl is dissolved in 100 mL distilled water to prepare for 4% (w/v) NaCl solution.

    [Prepare for 5 mL]

    3. An 80% solution is prepared from 100 mL of 95% EtOH. 11.75 mL of distilled water is

    added to 100 mL of 95% EtOH.

    4. 400 mL of 1.20 M solution of sodium chloride is prepared. [Prepare for 10 mL]

    (B) Preparation of Buffers

    Using the calculation method, the following buffers were prepared:

    i.) 1 L of 0.1 M Tris-HCl pH 7 [Prepare for 100 mL]

    ii.) 100 mL of 0.1 M Tris-HCl pH 4 [Prepare for 50 mL]

    iii.) 100mLof0.3MTris-HClpH7 [Preparefor50mL]

    iv.) 100 mL of 0.4 M Tris-HCl pH 7 [Prepare for 50 mL]

    v.) 500 mL of 0.1 M NaOH [Prepare for 100 mL]

    Results
    (A) Preparation of Solutions

    (B) Preparation of Buffers


    Discussion

    Tris, short for tris(hydroxmethyl)aminomethane is a primary amino compound

    that is tert-butylamine in which one hydrogen attached to each methyl group is replaced

    by a hydroxy group. It has a role as a buffer. It is a triol and a primary amino compound.

    It is a conjugate base of a member of Htris. A compound widely used as a biological

    buffer substance in the pH range 7--9; pKa = 8.3 at 20 ℃; pKa = 7.82 at 37 ℃. (Source:

    Pubchem)

    2D diagram of Tris

    Among the precautions to be taken in this experiment is the naming of the

    solutions. The name of the solution is to be labeled clearly on a sticker. The sticker needs

    to be pasted on the body of the bottle containing the solution, not the cap of the bottle.

    This is to ensure that the different solutions will not be mixed up when the caps are

    removed. Your name and the date by which the solution was prepared should also be

    written on the sticker.


    As seen in the preparation of 80% EtOH solution, we are able to calculate the

    final volume of 95% EtOH needed. By using the formula, M 1V1=M2V2 𑁋 M1 stands for

    the concentration of stock reagent, V1 stands for the volume of the stock reagent, both M2

    and V2 respectively stand for the final concentration and volume needed. When given the

    concentration of the EtOH which is 95% and its volume which is 100mL as well as the

    final concentration wanted, we are able to calculate the volume of EtOH needed.

    Minusing the volume of EtOH needed from the initial volume gives us the volume of

    distilled water needed for dilution.

    Conclusion

    The preparation of solutions and buffers is a common and vital task done in a laboratory

    setting. Different solutions are needed to carry out various different experiments. A solution is

    formed when a solute is evenly and uniformly distributed in a solvent. The ratio of solute to

    solvent affects the concentration of the final solution. The preparation of buffers is also a

    common task done in labs, especially in fields of biological sciences. A buffer is a solution that

    is able to resist change in pH, attributed to it containing species within the solution that is able to

    react with any added acid or base. Through this experiment, the understanding of the basic

    principles of preparing solutions and buffers were achieved. The appropriate formula were used

    to calculate the amount of solutes or solvents needed as well as it concentrations.

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