Libra S11 and S12

User Manual

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 Biochrom Ltd
Certificate No. 890333

Declaration of Conformity
This is to certify that the Libra S11 Visible and Libra 12 UV/Visible
Spectrophotometers
Part number 80-2115-15 and 80-2115-10
Serial number 81000 onwards

manufactured by Biochrom Ltd. conform to the requirements of the following

Directives-: 73/23/EEC & 89/336/EEC

Standards to which conformity is declared

EN 61 010-1: 2001
Safety requirements for electrical equipment for measurement, control and
laboratory use.

EN 61326: 1998
Electrical equipment for measurement, control and laboratory use – EMC
requirements

Signed: Dated: 23nd October 2002

David Parr
Managing Director
Biochrom Ltd

Postal address Telephone Telefax

Biochrom Ltd +44 1223 423723 +44 1223 420164

22 Cambridge Science Park
Milton Road e mail: enquiries@biochrom.co.uk website: http://www.biochrom.co.uk
Cambridge CB4 0FJ
England

Registered in England No: 3526954

Registered Office: 22 Cambridge Science Park, Milton Road, Cambridge CB4 0FJ, England.
CONTENTS

Unpacking, Positioning and Installation 1

Essential Safety Notes 2

OPERATION 3
Introduction 3
Using the Instrument Display and Keypad 4
Customisation of the instrument menu 5
Basic Modes of Use 6
Enhanced Modes of Use 9
Method storage, recall and deletion 13

SET-UP 15
Menu customisation, access code and methods 15
Lamp settings 16
Display contrast and instrument output 16

ERROR MESSAGES 17
OUTPUT OF RESULTS 18
Use with parallel printer 18
Use with chart recorder 18
Use with PC 18

ACCESSORIES 19
Lamps, consumables and other items 19

MAINTENANCE 20
After Sales Support 20
Lamp Replacement 20
Deuterium Lamp Warranty (Libra S12) 23
Fuse replacement 23
Cleaning and general care of the instrument 23

APPENDIX 24
Equation entry using the Multi Wavelength mode 24

SPECIFICATION 25
Warranty 26
Unpacking, Positioning and Installation

• Inspect the instrument for any signs of damage caused in transit. If any damage
is discovered, inform your supplier immediately. Check the position of the
metal lamp bracket inside the lamp access area.

• Ensure your proposed installation site conforms to the environmental conditions

for safe operation:

Indoor use only

Temperature 10°C to 40°C
Maximum relative humidity of 80 % up to 31°C decreasing linearly to 50 %
at 40°C

• The instrument must be placed on a hard, flat bench or table that can take its
weight (6 kg) such that air is allowed to circulate freely around the instrument.

• Ensure that the cooling fan inlets and outlets are not obstructed; position at least
2 inches from the wall.

• This equipment must be connected to the power supply with the power cord
supplied and MUST BE EARTHED (GROUNDED). It can be used on 90 -
240V supplies.

• Switch on the instrument. Prior to calibration, the display asks you to check that
the cell compartment is clear. The purpose of this is to indicate the use of the
function soft keys, and how they are associated with the options presented at the
bottom of the display; F2 represents OK in this instance (this display can be
disabled in Set-up if required). The calibration stages are indicated in sequence
(- for checking, 9 for OK, 8 for Fail).

• At switch on the language of the instrument can be changed if required. The

relevant key below should be pressed repeatedly while the status bar is active
(this is only for a couple of seconds immediately after switch on). The
following numbers correspond to the languages available:

0 English 1 German 2 French 3 Spanish 4 Italian

If this equipment is used in a manner not specified or in environmental conditions

not appropriate for safe operation, the protection provided by the equipment may be
impaired and instrument warranty withdrawn.

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Issue 04 - 07/2006 Libra S11/S12, English 1
Essential Safety Notes
There are a number of warning labels and symbols on your instrument. These are
there to inform you where potential danger exists or particular caution is required.
Before commencing installation, please take time to familiarise yourself with these
symbols and their meaning.

Caution (refer to accompanying documents).

Background colour yellow, symbol and outline black.

WARNING WARNING

UV RADIATION UV RADIATION IS HARMFUL TO YOUR EYES

HOT If power is restored with this cover removed,
eye protection must be worn

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2 Libra S11/S12, English Issue 04 – 07/2006
OPERATION
Introduction
Your spectrophotometer is a simple-to-use, microprocessor-controlled instrument.
In addition to the basic modes of operation, the instrument has enhanced software
and method storage functionality. A laboratory technician or supervisor can
customise the spectrophotometer for students and operators by disabling menu
options that are not required.

Your spectrophotometer:

• has basic modes of operation -

♦ measurement of absorbance, % transmission and concentration values,

♦ output of simple kinetics assays and wavelength scans to display.

• has enhanced modes of operation -

♦ the facility to enter a multi point standard curve in memory

♦ the application of a factor to an absorbance change over a specified time
interval for an enzymatic determination (reaction rate)
♦ the use of absorbance values in a multi wavelength equation specified by
you, with direct output of results, saving post run calculation

• stores up to 9 user defined methods

• can have any combination of the above, including methods, enabled so as to

customise the instrument for your own specific laboratory needs

• can be connected to a standard Centronics parallel printer for output of results

• can be linked via a serial interface adapter lead to a PC for download of results
to spreadsheet, and subsequent inclusion in a laboratory information
management system (LIMS)

A range of accessories further enhances the capability of the instrument.

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Issue 04 - 07/2006 Libra S11/S12, English 3
Using the Instrument Display and Keypad
The back-lit liquid crystal display has large characters which are easily visible,
useful if a group of students are gathered around for a demonstration, for example.
The keypad is a spill proof membrane that is very hard wearing.

The instrument is easy to use, with function select / entry soft keys on the keypad
(F1, F2 and F3) being situated directly below the corresponding option on the
display; these keys are used in conjunction with on-screen prompts.

On the absorbance home page, for example, concise help text is available at the push
of the F1 key, whereas Menu and Set-up are accessed by F2 and F3, respectively.
On other displays, the purpose of the keys changes, but this is clearly indicated; for
example, F3 acts as the “accept” option on parameter entry displays and next page (if
further options exist) in the Menu and Set-up displays.

Pressing the red stop key acts as an escape mechanism in most situations.

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4 Libra S11/S12, English Issue 04 – 07/2006
Press:

← to clear incorrect user entries from the display.

λ to enter the wavelength at which the instrument is to be used.

to set reference of absorbance to 0.000AU on a reference solution at the

current wavelength in the mode selected. Where it is standard operating
procedure, the user is prompted to insert a cell containing a reference into
the cell holder.

to start making measurements or print results.

to stop making measurements or return to the initial parameter screen

within the current operating mode.

• to print result

Esc to stop an experiment (time intervals and wavescan only)

OK To go to the absorbance page after calibration or from set-up.

Customisation of the instrument menu

Customisation of the menu to suit laboratory needs is an important benefit of the
instrument. This facility is password protected so that only authorised personnel are
able to set up or change the instrument.

• In a teaching laboratory, a laboratory technician might choose to have only the

modes of Absorbance, Factor Concentration, Time Intervals and Scan available.
• In the QC lab, the supervisor may choose to have Absorbance, Standard
Concentration, Multi-point Standard Curve entry and Reaction Rate.
• Similarly, a production line might have Absorbance and two methods as the
instrument start up; in this case, the methods could both be multi wavelength
equations involving factors, and set up in the analytical laboratory for use by
operators.

To customise the instrument, refer to Set-up > Menu for further details.

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Issue 04 - 07/2006 Libra S11/S12, English 5
Basic Modes of Use

Absorbance

Absorbance mode is default after power on and calibration. It makes simple

absorbance measurements on samples, measuring the amount of light that has passed
through a sample relative to a blank (this can be air). The procedure is as follows:

Press λ key and enter appropriate wavelength

Insert reference, press key.
This reference value is used for subsequent samples until changed.
Insert samples as required and record the absorbance.

% Transmission

Transmission mode measures the amount of light that has passed through a sample
relative to a blank (this can be air), but displays the result as a percentage. The
procedure is as follows:

Press λ key and enter appropriate wavelength

Insert reference, press key
This reference value is used for subsequent samples until changed.
Insert samples as required and record the transmittance.

Concentration

Factor concentration mode is used when a conversion factor is known, and is

required to convert the absorbance measurement for a sample at a specific
wavelength into a concentration, by a simple multiplication of absorbance x factor.
The procedure is as follows:

Enter appropriate wavelength

Enter known factor (range 0.01-99999)
Insert reference, press key
This reference value is used for subsequent samples until changed.
If you wish to save as a method, go to set-up (F3)
Insert samples as required and record the concentrations.

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6 Libra S11/S12, English Issue 04 – 07/2006
Time Intervals

Simple kinetics studies for teaching laboratory experiments can be readily

performed. The wavelength of interest is entered together with the time interval at
which absorbances are to be read; the option of having a reference reading prior to
the run is available. A count down facility indicates the time remaining until the next
measurement. To end an experiment, the stop key is pressed. The procedure is as
follows:

Enter appropriate wavelength

Enter time unit (seconds or minutes)
Enter end time (≤ 10,000)
Enter the time interval for each reading (range 1-60 seconds). A minimum of 10
data points are required, and the time interval for this is calculated.
If you require a reference reading press F3 (if not, press F2)
Insert reference, press key
This reference value is used for subsequent samples until changed.
If you wish to save as a method, go to set-up (F3)
Insert sample, press key
Press . to print graphic
Press key when the experiment is complete

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Issue 04 - 07/2006 Libra S11/S12, English 7
Wavescan

An absorption spectrum can be obtained from your instrument; this enables simple
identification of peak height and position. A reference scan has to be obtained first
since there is no stored baseline. The procedure is as follows:

Enter start wavelength

Enter end wavelength (nearest 10, 20, 50 or 100 nm to start wavelength)
Select Absorbance or Transmission mode
Insert reference, press key
This reference value is used for subsequent samples until changed.
If you wish to save as a method, go to set-up (F3)
Insert sample, press key (repeat as necessary)
Press . to print graphic

Use the 3 (F1) and 4 (F3) keys to move the cursor in order to identify peak height
and position.
To zoom in on a region, press F2 followed by the start and end wavelengths (the
instrument will zoom to the nearest 10, 20, 50 or 100 nm).

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8 Libra S11/S12, English Issue 04 – 07/2006
Enhanced Modes of Use

Standard Concentration

Standard Concentration mode is used when a sample of known concentration is

available; by measuring the absorbance of this at a specific wavelength, the
conversion factor is calculated (see above), and this can be applied to other samples
of unknown concentration. This is equivalent to a one point calibration, and assumes
that a sample of zero concentration has zero absorbance. The procedure is as
follows:

Enter appropriate wavelength

Enter concentration of known standard
Insert reference, press key
This reference value is used for subsequent samples until changed.
Insert standard, press
The absorbance value is displayed; press F2.
If you wish to save as a method, go to set-up (F3)
Insert samples as required and record the concentrations relative to the standard.

If recalling as a method, set reference before measuring samples.

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Issue 04 - 07/2006 Libra S11/S12, English 9
Standard Curve

The construction of a multi point calibration curve from standards of known

concentration in order to quantify unknown samples is a fundamental use of a
spectrophotometer; this instrument has the advantage of being able to store this curve
as a method. The procedure to construct the standard curve is as follows:

Select whether cubic spline or linear regression fit of the data points is required
Enter appropriate wavelength
Input the number of standards to be used:

For cubic spline fit, a minimum of 4 standards is required; maximum is 10.

For linear regression, a minimum of 3 data points is required (if 1 is
entered, the mode reverts to standard concentration); maximum is 10.

Enter the concentrations of the standards in increasing value *.

Insert reference, press key
This reference value is used for subsequent samples until changed.
Insert standard 1 of known concentration, press
The absorbance is displayed; press F2 to proceed to next standard.
Repeat as necessary for all standards
Press . to print graphic
The display shows - - - -, signifying that the standard curve has been defined, and
that samples can now be measured.
If you wish to save as a method, go to set-up (F3)
Insert samples as required and record the concentrations relative to the standard
curve.
Any sample absorbance / concentration which is outside the limits defined by the
standards used is displayed as - - - - .

If recalling as a method, set reference before measuring samples. The display

continues to show - - - - after the set reference. To recall stored method parameters
only, for example in protein assays where freshly prepared standards are frequently
used with new samples, press STOP after method recall. Press enter to move
through the stored method parameters, and measure the absorbances of the fresh
standards in the usual way; these new values are used in the standard curve.

- To include a zero concentration standard, include this in the number of

standards to be entered and enter 0.00 for concentration; use a blank when
required to enter standard 1. If using duplicates, enter the same concentration
twice; 2 duplicates of 3 different concentration equals 6 standards.

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10 Libra S11/S12, English Issue 04 – 07/2006
Reaction Rate

Reagent test kits are routinely used for the enzymatic determination of compounds in
food, beverage and clinical laboratories by measuring NAD / NADH conversion at
340 nm. The change in absorbance over a specified time period can be used to
provide useful information when an appropriate factor, defined in the reagent kit
protocol, is applied.
Note that reaction rate and enzyme activity can be calculated if the factor used takes
account of the absorbance difference per unit time, as opposed to the absorbance
difference per se.
The correlation (quality of line fit) is calculated from 10 equally spaced absorbance /
time points during the course of the experiment. The procedure is as follows:

Enter appropriate wavelength

Enter time unit (seconds or minutes)
Enter delay time, if applicable (0 ≤ 600)
Enter end time (≤ 10,000)
Enter factor (range 0.01-99999)
If you require a reference reading press F3 (if not, press F2)
Insert reference, press key
This reference value is used for subsequent samples until changed.
If you wish to save as a method, go to set-up (F3)
Insert sample, press key.
The display indicates the change in absorbance for each of the calculated time
intervals as the assay proceeds.
The result (total change in absorbance over the reaction time multiplied by the
factor) is displayed; press F2 to display the correlation (a correlation of > 0.95 is
expected if the assay was carried out over a linear section).

If recalling as a method, set reference before measuring samples.

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Issue 04 - 07/2006 Libra S11/S12, English 11
Multi Wavelength Equation Entry

The measurement of Absorbance values at specific wavelengths and combining these with
appropriate factors is a means of overcoming interference effects in several applications. By
using the equation entry facility, post measurement calculations can be done automatically
and the end result displayed for the operator. This is a very powerful facility indeed for the
busy industrial, QC or environmental testing laboratory. Up to 5 wavelengths and 6 constants
(5 factors relating to the absorbances at the 5 wavelengths, and a dilution factor) can be
entered for one equation. The procedure is as follows (refer to Appendix for a step by step,
worked through example):

Write the equation out in front of you, ensuring there are no syntax errors!
The available equation operators are:
A@1 Absorbance at wavelength 1. Enter required wavelength using keypad.
T@1 Transmission at wavelength 1. Enter required wavelength using keypad.
( ) + - * /
K1 Factor applied to absorbance at wavelength 1. Enter using keypad.
C1 Constant (dilution or other). Enter using keypad. Note that C1 can be applied
several times, using different numerical values each occasion.
! Use if a sequence of Absorbance values only is required Enter !A@1 A@2 etc

Note that Factor and Constant can only have 5 characters, including the decimal point; thus
12.259 is not allowed, whereas 0.302 is.
The maximum length of equation that can be accommodated is 60 characters in length, where
the absorbance at wavelength and factor are 3 and 5 characters, respectively, and the equation
operators are 1 character.

Select Absorbance or Transmission mode

Press Next (F1) to obtain the required parameter, using the keypad for absorbance values and
factors, as appropriate.
Press F2 to select the parameter and move on to the next one.
Repeat this procedure until the equation is entered.
Note that if you make an error, the ← key on the keypad will remove the last entry.
Press F3 to enter the equation once it has been input correctly.
Insert reference, press key. A set reference at each of the required wavelengths is taken.
If you wish to save as a method, go to set-up (F3)

Insert sample, press key.

The result of the calculations involved in the defined equation is displayed.
Press F2 to proceed to next sample
If recalling as a method, set reference before measuring samples.

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12 Libra S11/S12, English Issue 04 – 07/2006
Ratio

This facility enables the determination of Abs λ1 / Abs λ2 and Abs λ*factor

Enter the first wavelength

Enter the second wavelength
Select if background correction (for both wavelengths) is required
If yes, enter the wavelength
Enter the factor to be applied to the first wavelength
Enter dilution factor (range 1.0 – 99999)
Insert reference, press key. A set reference at each of the required wavelengths
is taken. Press F2.
If you wish to save as a method, go to set-up (F3)
Insert sample, press key.
The results for absorbance values, concentration and the ratio are displayed. Press
F2 to proceed to next sample
If recalling as a method, set reference before measuring samples.

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Issue 04 - 07/2006 Libra S11/S12, English 13
Method storage, recall and deletion
After defining parameters in any of the above modes, and prior to measuring a
sample, entry to Set-up using the F3 function key provides the opportunity to store
the parameters currently loaded as a method. This option is password protected, and
up to 9 methods can be saved; refer to Set-up > Methods for further details.

A stored method is enabled as an option directly on the instrument menu, so that it is

possible for an operator to switch the instrument on and have a specified method
available straight away; refer to Set-up > Menu for further details.

When recalling a stored method from the menu, the option to print the method
parameters is presented by pressing 1; press 2 to continue with the selected method.
Print the method to confirm that it is the method you require, if necessary. Once a
method has been recalled, you can set reference before running samples.

If method parameters are incorrect, they cannot be changed – the method has to be
erased (deleted), re-entered and then re-stored (re-saved). To remove a method,
refer to Set-up > Methods.

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14 Libra S11/S12, English Issue 04 – 07/2006
SET-UP

After selecting the set-up option (F3) there is an initial information screen, as shown
below. Press F2 (OK) to return to the absorbance home page. Press F1 to
recalibrate the instrument.

Serial # Serial Number of the instrument

6020 or 6040 V1.0 Product line number, version of EPROM
UV lamp hours Total UV lamp life used (Libra S12)
Vis lamp hours Total visible lamp life used
Instrument hours Total instrument hours

To access the set-up page press F3 again. A password is required; the default is
6020 or 6040, but this can be changed.

Three displays are available: Menu customisation, access code and methods
Lamp settings
Display contrast and instrument output

To go to the next display press F3.

To change or select an option press the relevant number on the keypad. Another
display screen may appear depending on the option.
Use the function soft keys in conjunction with the options indicated by the display,
as appropriate.

Menu customisation, access code and methods

1 : Menu Press 1 to obtain a list of all the modes. These can be enabled
or disabled as required by pressing the relevant number on the
keypad. Disabled options are not shown on the main menu
display.
2 : All Menu Press 2 to show disabled options from above as greyed out text
on the main menu, even though they cannot be selected.
3 . Access Code Press 3 for the possibility to change the password from the
default to another 4 digit number. Enter the current password
(Access Code), then the new one (Access Code #1) and
confirm the new one (Access Code #2). If you forget the
password, contact your supplier.

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Issue 04 - 07/2006 Libra S11/S12, English 15
4 : Methods Press 4 to have the choice of either storing a new method
after defining parameters or erasing an existing method.
Press 1 to store the method in the next available method
storage space (maximum is 9). Methods are stored in the
instrument EEPROM, the process may take a few seconds.
Press 2 to erase a method; the method number has to be
entered.
Method parameters can be printed out when the stored
method is selected from the menu.
Lamp settings
1 : UV lamp Switch deuterium lamp on/off (Libra S12)
2 : Vis lamp only Deuterium lamp is switched permanently off; and the
instrument will act as a visible only product. Infrequent
users of the UV range can benefit from considerable
increase in deuterium lamp life by use of this utility.
3 : UV lamp save Instrument powers up and calibrates as usual, but then
switches off the deuterium lamp automatically. The lamp
will come on if a UV wavelength is selected (Libra S12)
4 : Lamp hours ⇒ 0 Resets lamp lives to zero when a new lamp is fitted.

Display contrast and instrument output

1 : ⇑ Contrast Increase display contrast one step at a time by pressing 1

2 : ⇓ Contrast Decrease display contrast one step at a time by pressing 2
(a total of seven contrast levels are available)
3 : Calibration Menu Disables the “Ensure cell compartment is clear” message
which appears prior to calibration.
4 : Output to serial Enables output of ASCII datastream to PC via the serial
interface adapter lead. The information is in tab separated
format.
5 : Output to printer Enables output to parallel printer via a standard Centronics
cable; text only, Seiko DPU-414, HP DeskJet (A4), HP
DeskJet (letter).

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16 Libra S11/S12, English Issue 04 – 07/2006
ERROR MESSAGES

The following are a selection of error messages that are available:

Reset to defaults Memory was corrupted in some way and re-set to defaults.
Display options and methods need to be re-entered.
UV lamp fail UV lamp failed to strike. May need replacing – check lamp
hours (Libra S12)
Vis lamp fail Visible lamp failed to strike. May need replacing – check
lamp hours
Beam blocked Something is in the way of the beam – check sample
compartment area
Wavelength error A calibration failure or corruption has caused the instrument
to go an invalid wavelength. May need service engineer.
Lamps overheating The thermal sensor on the lamp cover has detected a
temperature in excess of its limits – call service engineer.
PSU overheating PSU thermistor is indicating a temperature in excess of
70°C – call service engineer.

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Issue 04 - 07/2006 Libra S11/S12, English 17
OUTPUT OF RESULTS
Use with parallel printer
Any Centronics parallel printer can be used together with the appropriate cable. If
using a thermal printer, ensure it is set up to print out for a page width of 80
characters. Ensure output to printer is on in the Set-up.
Output is automatic when the key is pressed, and a printer is connected and
switched on. Umlauts and accents are not printed out with letters if the instrument is
set up to be in German, French, Italian or Spanish.
Appropriate headers and relevant information are printed out for enhanced modes,
for example the absorbance – concentration values of the standards in standard curve
mode, and the equation (with values) entered in Multi Wavelength mode.

Use with chart recorder

The chart recorder interface lead, 80-2109-03, is required; time intervals and scan
modes only will output to a chart recorder in a meaningful way. The output is via
pin 24 (+) and pin 25 (-) of the 25 pin D connector if you wish to make your own.
Output is non-synchronised, that is the chart recorder must be switched to run
independently. The output is 100 mV for 1.000 abs unit, and a suggested chart
speed is 10 mm / second. Note that an offset is required; - 0.5 Abs = 0 mV, 0.0 Abs
= 50 mV, 3.0 Abs = 350 mV (use Absorbance mode to set these pen positions on the
chart recorder).

Use with PC
NOTE: A standard serial interface will not work.
1) Download to Spreadsheet
The serial interface adapter lead (80-2109-02) is required; it is also supplied with
Spreadsheet Interface Software for direct download to Excel. This macro is supplied
on a floppy disc together with instructions for installation and use.
2) Use with Hyperterminal
The serial interface adapter lead (80-2109-02) is required; ensure output to serial is
on in Set-up. The ASCII stream is output at 19,200 Baud via the 25 way D
connector on the rear panel, and can be picked up by a PC with Windows installed.
Use the Hyperterminal emulator in Accessories to pick this up (settings are
Handshake None, 19,200 Baud, 1 stop bit, 8 data bits, 0 parity, Comm port depends
on which port the lead is connected to). Output is automatic if the interface lead is
connected to the instrument.

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18 Libra S11/S12, English Issue 04 – 07/2006
ACCESSORIES

Each accessory is supplied integrated into its own sample compartment for ease of
fitting and cleaning.
Easy to fit - when changing accessory / sample compartment, snap the old one
out and the new one in.
Easy to clean - take the whole assembly out and run it under the tap.

Acquire Lite applications software 80-2112-24

Manual 2 position 10mm cell changer 80-2109-04
10 – 50mm cell holder 80-2109-05
Water heated cell holder 80-2109-06
(requires circulating bath)
Electrically heated cell holder 80-2109-07
(requires Temperature Controller)
Temperature Controller (25, 30, 37°C) 80-2112-54
Fitting kit for external sample delivery 80-2109-08
(requires peristaltic pump and 10mm pathlength flowcell)
Test tube holder and cover 80-2109-33
(accommodates diameters of 8-26 mm and heights of up to 180 mm)
Spare 10mm single cell holder 80-2109-09
Cylindrical cell holder 80-2112-26
(50mm pathlength cylindrical cells)
Lamps, consumables and other items
Tungsten halogen lamp (Libra S12) 80-2106-16
Tungsten halogen lamp (Libra S11) 80-2022-94
Deuterium lamp (Libra S12) 80-2109-11
Serial Interface Adapter Lead 80-2109-02
(includes Spreadsheet Interface Software)
Chart Recorder Lead 80-2109-03
Centronics parallel printer lead 80-2071-87
Dust cover 80-2109-13

Contact your supplier for details on our range of disposable, UV silica and glass
cells.

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Issue 04 - 07/2006 Libra S11/S12, English 19
MAINTENANCE
After Sales Support
We supply support agreements that help you to fulfil the demands of regulatory
guidelines concerning GLP/GMP.

♦ Calibration, certification using filters traceable to international standards

♦ Certificated engineers and calibrated test equipment
♦ Approved to ISO 9001 standard

Choice of agreement apart from break down coverage can include

♦ Preventative maintenance
♦ Certification

When using calibration standard filters, insert such that the flat surface is facing
away from the spring end of the cell holder

Observe all necessary precautions if dealing with hazardous samples or solvents.

Lamp Replacement
Replacement lamps are available from your supplier using the following part
numbers:

Deuterium Lamp 80-2109-11 (Libra S12)

Tungsten Lamp 80-2106-16 (Libra S12), 80-2022-94 (Libra S11)
(use only this tungsten lamp; others will not operate correctly in a
spectrophotometer)

The design of the lamp area is such that users are able to change their own lamps.
No lamp alignment is necessary as the lamps are pre-aligned at manufacture.

The lamps become very hot in use. Ensure they cool before changing them.

Do not touch the optical surfaces of either lamp with your fingers (use tissue); if
touched, the area should be cleaned with iso-propanol.

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20 Libra S11/S12, English Issue 04 – 07/2006
To replace a lamp proceed as follows:
1) Switch off the instrument, remove the sample from the cell holder and
disconnect the power supply cord.
2) Locate the lamp access cover at the back of the instrument, unscrew the cover
and remove.
3) Move the metal bracket sideways, slide the lamp plate assembly out and
unplug the connector.
- if the tungsten lamp has failed, the replacement should be inserted onto the
plate, pushing it all the way down into its holder.
- if the deuterium lamp has failed, insert the old tungsten lamp onto the plate as
above and then replace the whole assembly with the new one.
4) Reconnect the cable connector and slide the lamp plate in until it locates,
checking that the cable or connector does not interfere with the relocation.
5) If you have difficulty in sliding the lamp assembly back into position hold the
connector down and push the lamp plate until it locates correctly.
6) Replace the lamp access cover.
7) Reconnect the power supply cord and switch the instrument on.
8) Reset the lamp life to zero by:
F3 Set-up → F3 Set-up → enter password → F3, F3 → select 4 → F3
(9), → select which lamp life is to be changed to zero.
Exit this screen by pressing the key
Exit set-up by pressing the key.

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Issue 04 - 07/2006 Libra S11/S12, English 21
Lamp Replacement Libra S11 Only – proceed as follows
Tungsten Lamp 80-2022-94 (Libra S11)

(use only this tungsten lamp; others will not operate correctly in a this
spectrophotometer)

To replace a lamp proceed as follows:

1) Switch off the instrument, remove the sample from the cell holder and
disconnect the power supply cord.
2) Locate the lamp access cover at the back of the instrument, unscrew the cover
and remove.
3) Using a lens cloth, grip the lamp and pull it out. This is achieved more easily
by gently pulling on the two brown wires at the same time, to release the
springs holding the lamp in place.

4) The replacement tungsten lamp should be inserted onto the plate, pushing it
all the way down into its holder. Use a lens cloth to hold the new lamp. Again
the brown wires can be gripped and pulled towards you to ease this process.
Ensure the lamp is located as far inside the holder as possible. It should also
appear horizontally positioned.
5) Replace the lamp access cover.
6) Reconnect the power supply cord and switch the instrument on.
7) Reset the lamp life to zero by:
F3 Set-up → F3 Set-up → enter password → F3, F3 → select 4 → F3 (9),
→ select which
lamp life is to be changed to zero.
Exit this screen by pressing the key
Exit set-up by pressing the key.

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22 Libra S11/S12, English Issue 04 – 07/2006
Deuterium Lamp Warranty (Libra S12)
Criteria for lamp replacement are that it must:

- be less than 15 months old AND

- have had less than 750 hours use

Fuse replacement
Switch off the instrument and disconnect the power supply cord. The fuse holder
can only be opened if the power supply plug has been removed, and is located
between the power input socket and the on/off switch on the back panel of the
instrument.

Slide open the fuse holder by pulling at the notch.

Place fuses (2A, 5mm x 20mm, FST) into the fuse holder and slide back into
position.

Reconnect the power supply cord and switch on the instrument.

Fuses are not normally consumed in an instrument’s lifetime. If they blow

repeatedly, contact your supplier.

Cleaning and general care of the instrument

External cleaning
Switch off the instrument and disconnect the power cord.
Use a soft damp cloth.
Clean all external surfaces.
A mild liquid detergent may be used to remove stubborn marks.

Sample compartment spillage

Switch off the instrument and disconnect the power cord.
Remove the cell holder by snapping it out.
Clean it separately with a soft damp cloth, or hold it under running water from a tap.

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Issue 04 - 07/2006 Libra S11/S12, English 23
APPENDIX
Equation entry using the Multi Wavelength mode
Always write out the equation in front of you before using this mode.

Step by step entry of the following equation is shown in the example below:

Cobalt (g/l) = ( (A511 * 12.26) – (A720 * 0.302) ) * 100

Note that if you make an error, the ← key on the keypad will remove the last entry.

Press Next (F1) until ( appears. Press select (F2).

Press Next (F1) until ( appears. Press select (F2).
Press Next (F1) until A@1 appears. Press select (F2).
Press 511 on the keypad. Press enter (F3).
Press Next (F1) until * appears. Press select (F2).
Press Next (F1) until K1 appears. Press select (F2).
Press 12.26 on the keypad. Press enter (F3).
Press Next (F1) until ) appears. Press select (F2).
Press Next (F1) until – appears. Press select (F2).
Press Next (F1) until ( appears. Press select (F2).
Press Next (F1) until A@2 appears. Press select (F2).
Press 720 on the keypad. Press enter (F3).
Press Next (F1) until * appears. Press select (F2).
Press Next (F1) until K2 appears. Press select (F2).
Press 0.302 on the keypad. Press enter (F3).
Press Next (F1) until ) appears. Press select (F2).
Press Next (F1) until ) appears. Press select (F2).
Press Next (F1) until * appears. Press select (F2).
Press Next (F1) until C1 appears. Press select (F2).
Press 100 on the keypad. Press enter (F3).

Check the equation on the display.

Press F3 to accept the equation

To save the equation as a method, refer to Set-up.

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24 Libra S11/S12, English Issue 04 – 07/2006
SPECIFICATION

Wavelength range 325- 999 nm (Libra S11) or

200 – 999 nm (Libra S12)
Monochromator Plane grating with 1200 lines/mm
Wavelength calibration automatic upon switch on
Spectral bandwidth 5 nm
Wavelength accuracy ± 2nm
Wavelength reproducibility ± 0.5nm
Light sources tungsten halogen and
deuterium arc (Libra S12)
Detector single solid state silicon photodiode
Photometric range - 0.300 to 3.000A, 0.01 to 99999 concentration units,
0.1 to 200%T
Photometric linearity ±0.5% or ± 0.005A to 2.000A at 546nm, whichever is
the greater
Photometric reproducibility 0.5% of absorbance value to 2.000A at 546nm
Stray Light typically <0.2%T at 220nm using NaI, <0.2%T at
340nm using NaNO2 according to ANSI/ASTM E387-
72
Stability ± 0.002A/h at 0A and 546nm after warm-up, typically
30 minutes
Noise ± 0.001A near 0A and ± 0.002A near 2A at 600nm
Scan speed 250 nm/minute
Analogue output 100mV per 1.000A via interface lead
Digital output Centronics parallel as standard
9 pin serial via interface adapter lead
Dimensions 310 x 400 x 180 mm
Weight 6 kg
Power requirements 90-265 V, 50/60 Hz, 100 VA

Safety standard EN61010-1

EMC emissions EN 61326-2.3 Generic emissions
EMC immunity EN 61000-4-6 Generic immunity part 1
Mains harmonics EN 61000-3-2
Susceptibility standard IEC 801
Quality System Designed and manufactured in accordance with an
ISO 9001 approved quality system

Specifications are measured after the instrument has warmed up at a constant

ambient temperature and are typical of a production unit. As part of our policy of
continuous development, we reserve the right to alter specifications without notice.

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Issue 04 - 07/2006 Libra S11/S12, English 25
Warranty
Your supplier guarantees that the product supplied has been thoroughly tested to
ensure that it meets its published specification. The warranty included in the
conditions of supply is valid for 12 months only if the product has been used
according to the instructions supplied. They can accept no liability for loss or
damage, however caused, arising from the faulty or incorrect use of this product.

This product has been designed and manufactured by Biochrom Ltd, 22 Cambridge
Science Park, Milton Road, Cambridge CB4 0FJ, UK.

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26 Libra S11/S12, English Issue 04 – 07/2006