------------------------------------- BioChem LAB -------------------------------------

Ionic and Molecular Compounds c. When the difference between

1. The mechanism for ionic bond electronegativities of the atoms is
formation always involves the 1.5
transferring of: d. When the difference between
a. Electrons from nonmetallic the electronegativities of the
atoms to metallic atoms atoms is 1.0
b. Protons from the nucleus of 5. For which of the following pairs are
metallic atoms to the nucleus of the atoms most likely to form an ionic
nonmetallic atoms bond with each other?
c. Sufficient electrons to produce a. Sodium and magnesium
ions of equal but opposite b. Chlorine and oxygen
charge c. Calcium and chlorine
d. More than one correct d. Carbon and oxygen
response 6. Which of the following is true of ionic
e. No correct response compounds?
2. Which of the following occurs when a. They are liquids at room
an ionic bond is formed? temperature
a. Electrons are transferred from b. They are solids in which
the less electronegative element individual molecules are present
to the more electronegative c. They are solids in which both
element. cations and anions are present
b. Electrons are shared equally d. Depending on the compound
c. Electrons are shared unequally any one of the above may apply
d. Electrons are transferred from 7. Which pair of species is most likely to
the more electronegative form an ionic bond?
element to the less a. Two electrically charged
electronegative element species, one positive and one
3. Which of the following element s is negative
most electronegative? b. Two negatively charged
a. N species
b. C c. Two electrically neutral species
c. B d. Two positively charged species
d. O 8. In which of the following cases is a
4. Under which of the following polar covalent bond formed?
conditions is the A—B bond considered a. When an electron is transferred
to be ionic? from one atom to another
a. When the difference between b. When the electrons of a bond
the electronegativities of the are shared unequally by the two
atoms is 2.0 atoms
b. When A and B have the same c. When a metallic element forms
electronegativity a bond with a nonmetallic
element
 d. When the electrons of a bond
are shared equally by the two
atoms
9. Which of the following statements is b. Middle layer
generally true about the c. Cannot be determined
electronegativity? d. Lower layer
a. Electronegativity decreases as 4. What is the typical reaction
we move left to right and undergone by hydrocarbons that make
increases as we move top to them react with good sources of fuel
bottom when ignited in the presence of
b. Electronegativity increases as oxygen?
we move left to right and a. Combustion
increases as we move top to b. Unsaturation Test
bottom c. Elimination
c. Electronegativity increases as d. Radical Substitution
we move left to right and 5. An alkane that are joined in rings are
decreases as we move top to called:
bottom a. Aliphatic
d. Electronegativity decreases as b. Cycloalkanes
we move left to right and decreases as c. Benzene Rings
we move top to bottom d. Aromatic
10. Which of the following bonds is the 6. Potassium Permanganate Test is also
most polar? commonly called as:
a. H---F a. Bromine Test
b. H---Br b. Selective Test
c. H---Cl c. Baeyer Test
d. H---l d. Test for unsaturation
Benzene and Benzene Derivatives 7. Which of the ff. chemical reagents
1. The simplest form of alkyne, which is used in this virtual lab activity that has a
ethyne is commonly called as: high level of toxicity hazard?
a. Bromine a. Potassium Permanganate
b. Ethylene b. Sulfuric Acid
c. Acetylene c. Acetylene
d. Biogas d. Bromine
2. Which of the ff. samples will most likely 8. Hydrocarbons that do not contain the
react with Potassium Permanganate? benzene group are generally called:
a. Water a. Alkenes
b. Hexane b. Alkanes
c. Cyclohexene c. Alkynes
d. Toluene d. Aliphatic
3. When you mixed cyclohexane with
water, a layer is formed. At which layer
will you find the cyclohexane?
a. Upper layer
9. The behavior of hydrocarbons in term 5. Which of the following will give a
of its solubility in water is mainly caused positive test with Tollen’s reagent?
by their: a. Aldehydes
a. Chemical instability b. Ketones
b. Low boiling points c. Both a and b
c. Low intermolecular forces of d. Neither a nor b
attraction 6. What identification test should to
d. Non-polar nature perform to distinguish 2- Pentanone and
10. In the virtual lab activity, acetylene is 3-Pentanone?
produced from reacting water with a. Iodoform Test
___________: b. Bisulfite Test
a. Calcium oxide c. Sodium Nitroprusside Test
b. Carbon dioxide d. Phenylhydrazine Test
c. Oxygen e. All of the above
d. Calcium carbide 7. Which functional group is found in
Aldehydes and Ketones aldehydes?
1. Which test involves the ff. chemicals: a. RCOR’
Silver Nitrate, Sodium Hydroxide and b. RCOOH
Ammonium Hydroxide c. RCHO
a. Fehling’s Test d. RCH2OH
b. Tollen’s Test 8. In which of the following can
c. Schiff’s Test hydrogen bonding occur?
d. Sodium Bisulfite Test a. in all these cases
e. Iodoform Test b. in a solution of formaldehyde
2. Which of the following is obtained by in water
oxidizing hexanal? c. in a solution of acetone and
a. none of these formaldehyde in water
b. hexanoic acid d. in a solution of acetone in
c. 1-hexanol water
d. 2-hexanone 9. Which of the following compounds
3. Solubility in water (1-lowest, 4-highest will undergo oxidation using potassium
solubility) dichromate to form a carboxylic acid?
4 – Butanal
3 – Pentanal
2 – Hexanal
1 – Heptanal
4. Boiling point (1 – Highest, 4 – Lowest)
1 – 2-heptanone a. A and B only
2 - 2 – hexanone b. A and C only
3 – 2-pentanone c. B only
4 - 2-butanone d. C only
10. These are compounds that lose an c. a base
electron in the chemical reaction d. an oxidizing agent
a. Oxidizing Agent 2. Which of the following statements
b. Reducing Agent concerning the boiling points of
c. Tollen’s Reagent carboxylic acids is correct?
d. None of the above a. They are higher than those of
e. All of the above corresponding alcohols
11. Which compound gives a positive b. More than one correct
test to Sodium Nitroprusside Test? response
a. Acetone c. No correct response
b. 2 – Pentanone d. They are lower that those of
c. Butanone corresponding alkanes
d. All of the above e. They are lower than those of
e. None of the above corresponding aldehydes
12. Which structural feature is common 3. What would you expect is the order of
to aldehydes and ketones? water solubility for the following
a. An oxygen atom double compounds:
bonded to a carbon atom
b. Two oxygen atoms bonded to
the same carbon atom
c. An oxygen atom bonded to
a. B > A > C
two carbon atoms
b. A > C > B
d. An oxygen atom bonded to
c. C > A > B
both a carbon atom and a hydrogen
d. A > B > C
atom
4. Carboxylic acids can form hydrogen
13. Which functional group is found in
bonds with which of the following
ketones?
classes of compounds?
a. RCHO
a. All of these
b. RCOOH
b. Aldehydes
c. RCOR’
c. Alcohols
d. RCH2OH
d. Ethers
14. Which of the ff. compounds will have
5. Decarboxylation involves the loss of
a positive test with Iodoform Test?
which of the following small molecules?
a. Acetone
a. none of these
b. 2-Pentanone
b. H2CO2
c. Acetophenone
c. CO2
d. All of the above
d. CO
6. Which of the following compounds
Carboxylic Acids and Esters
will react with sodium hydroxide to form
1. Which of the following classes of
a water-soluble product?
reagents will convert a carboxylic acid
a. hexanal
into its carboxylate salt?
b. hexanoic acid
a. an acid
c. all of these compounds
b. a reducing agent
d. 1-hexene
7. Which of the following compounds d. They are significantly less
will have the lowest boiling point? soluble in water than the other
a. Butanoic acid compounds
b. Pentanoic acid 12. The reaction of carboxylic acid
c. Heptanoic acid which of the following reagents
d. Hexanoic acid produces a gas?
8. What is the name of the compound a. NaHCO3
produced by the reaction of benzoic b. Na2CO3
acid with sodium hydroxide? c. Both a and b
a. Sodium benzoate d. neither a nor b
b. sodium hydroxybenzoate 13. When compared with the boiling
c. benzoic sodate points of other neutral organic
d. sodium benzide compounds of comparable molecular
9. The ester obtained by reacting weight, which of the following is true of
methanol and ethanoic acid is called? the boiling points of carboxylic acids?
a. ethyl methanoate a. They are significantly higher
b. ethane methanoate than the boiling points of the
c. methyl ethane other compounds
d. methyl ethanoate b. They are slightly higher than
10. Given that the elctron-withdrawing the boiling points of the other
ability of a substituent falls off with compounds
distance, rank order the following c. They are significantly lower
carboxylic acids from strongest acid to than the boiling points of the
weakest acid: other compounds
d. They are slightly lower than the
boiling points of the other
compounds
a. (iii) > (ii) > (i)
14. What type of compound is formed
b. (i) > (ii) > (iii)
when a carboxylic acid reacts with an
c. (ii) > (i) > (iii)
alcohol in the presence of an acid
d. (ii) > (iii) > (i)
catalyst?
11. When compared with the solubility in
a. either a or b, depending on
water of other neutral organic
the conditions
compounds of comparable molecular
b. ester
weight, which of the following is true of
c. neither a nor b
the solubility of carboxylic acid?
d. ether
a. They are slightly less soluble in
15. In the Fischer esterification reaction,
water than the other
what catalyst is required?
compounds.
a. a strong acid
b. They are slightly more soluble in
b. a strong base
water than the other compounds
c. either a or b
c. They are significantly more
d. Neither a nor b, the reaction
soluble in water than the other
does not need a catalyst
compounds
Carboxylic Acids and Esters (Part II) 6. Most carboxylic acids are resistant to
1. What is the most acidic of the ff. oxidation, but under extreme conditions,
compounds: it may be oxidized to form:
a. 3-chloro-butanoic acid a. Esters
b. 2-chloro-butanoic acid b. Alcohols
c. 4-chloro-butanoic acid c. Carbon Dioxide
d. Butanoic acid d. Aldehydes
2. Which of the ff. reactions will convert 7. Addition of Sodium Hydroxide to
a carboxylic acid into its carboxylate carboxylic acids will turn litmus paper:
salt? a. From red to blue
a. Sodium Bicarbonate Test b. From blue to pink
b. Sodium Hydroxide Test c. From blue to red
c. Reaction with Hydrochloric d. No changes in the litmus paper
Acid 8. What is the name of the compound
d. A and B only produced by the reaction of n-pentanol
e. A, B and C and Ethanoic Acid (Acetic Acid) in the
3. This reaction of carboxylic acid will presence of a strong acid?
produce a gas: a. Isoamyl acetate
a. Reaction with Alcohol b. Ethyl Pentanoate
b. Reaction with Hydrochloric c. Pentyl Acetate
Acid d. Octyl Acetate
c. Sodium Bicarbonate Test 9. Which carboxylic acid/s will react with
d. Sodium Hydroxide Test Tollen’s Reagent?
4. In addition to the formation of esters, a. Formic Acid
what else is produced during Fischer b. Oxalic Acid
esterification? c. Lactic Acid
a. Alcohols d. A and B only
b. Oxygen e. A, b and c
c. Water 10. Which carboxylic acid/s will react
d. Carbon Dioxide with Potassium Permanganate?
5. What happened when a sodium a. Formic Acid
benzoate, a carboxylate salt is reacted b. Lactic Acid
with hydrochloric acid? c. Oxalic Acid
a. A white precipitate of benzoic d. All of the Above
acids is formed
b. The blue litmus paper turned LIPIDS
red 1. Triglycerides belong to which class of
c. Bubbles formed compounds?
d. A and B a. alcohols
e. A, B and C b. ethers
c. esters
d. carboxylic acids
2. The ff. is/are fat soluble Vitamins: 7. Identify what type of lipid is the ff.
a. Vitamin A compound:
b. Vitamin D Lecithin: Phospholipid
c. Vitamin E Beeswax: Waxes
d. All of the above Cholesterol: Steroid
3. Lipids perform which of the following Coconut Oil: Triglyceride
functions? Cerebroside: Glycolipid
a. They store energy within fat 8. How many rings are associated with
cells the steroid structure?
b. They serve as chemical a. 5
messengers. b. 6
c. They serve as membrane c. 3
components. d. 4
d. All of the above are functions 9. Which of the following explains the
of lipids. insolubility of lipids in water?
4. Cholesterol is used as which of the a. The polar portion of lipids is
following? much smaller than the nonpolar
a. a component of plasma portion
membranes b. The polar and nonpolar
b. a starting material for the portions of lipids are of
synthesis of other steroids comparable size.
c. a starting material for the c. The polar portion of lipids is
synthesis of bile salts much larger than the nonpolar
d. all of these portion.
5. These are the oxidation products of d. Lipids are polar molecules.
cholesterol and aid in the digestion of 10. Which of the following best describes
fats in our body. the components of phospholipids?
a. Vitamin E a. glycerol, one fatty acid, two
b. Lecithin phosphate groups, and an
c. Cerebrosides alcohol
d. Bile Salts b. glycerol, two fatty acids, a
6. Which of the ff. is true of an phosphate group, and an
Unsaturated Fat? alcohol
a. Most are vegetable fats and c. either of these, depending on
are liquid at room temperature the identity of the phospholipid
b. Has one or more double bonds d. none of the above
between carbons 11. Bile salts are mainly found in:
c. Will react with Hanus/Huble’s a. gallbladder
Reagent and causes b. blood
decolorization c. brain
d. all of these d. all of the above
12. Identify the name of the test being 4. What is the constituent that makes the
described in the given statement saliva slippery?
Test for the presence of Vitamin A a. Amylase
in the solution: Carr Price b. Glycoproteins
Reaction c. Mineral Salts
Test for the presence of d. Mucin
cholesterol: Lieberman-Burchard 5. Which of the ff. statement is always
Test true?
Test for the presence of a. Lowering the temperature will
phosphate in the solution: decrease the reaction rate of
Ammonium Molybdenum Test enzymes
Test for the presence of Vitamin E b. Increasing the substrate
in the solution: Modified Furter- concentration will always cause
Meyer Test an increase in the rate of
13. Which of the following is used to reaction
define lipids? c. All enzymes work well at near
a. the presence of a specific neutral pH
functional group d. An increase in temperature
b. their chemical structure can increase the reaction rate of
c. their insolubility in water enzymes
d. none of these 6. The enzyme salivary amylase is most
active at pH______.
SALIVARY DIGESTION a. 5.4
1. The digestion of food starts at the b. 10
_______. c. 6.8
a. Alimentary Canal d. 2.5
b. Mouth 7. The optimum temperature for the
c. Intestine enzymatic activity of salivary amylase
d. Stomach ranges from____ to _____.
2. These are the products of the a. -10°C to 10°C
enzymatic action of salivary amylase: b. 5°C to 10°C
a. Maltose c. 32°C to 37°C
b. Dextrins d. 30°C to 32°C
c. Glucose 8. Which of the following can cause
d. A and B only enzyme activity to either increase or
e. A,B and C decrease?
3. The glycoprotein material mainly a. changing the temperature
present in saliva, which can be b. increasing the substrate
precipitated by the addition of dilute concentration
acetic acid or by alcohol. c. both a and b
a. Dextrin d. neither a nor b
b. Maltose
c. Amylase
d. Mucin
9. Which of the following is true of c. At the lower temperature, the
enzymes? enzyme is more activated.
a. They shift the position of a d. At the lower temperature,
chemical equilibrium starch loss its functionality.
b. They speed up the rate of a 13. These are catalysts for various
chemical reaction biochemical reactions in the human
c. both a and b body.
d. neither a or b a. enzymes
10. What is an achromic point? b. nucleic acids
a. Time during the action of c. hormones
amylase on starch at which the d. proteins
reaction mixture no longer gives 14. The action of amylase in starch
a colour with Iodine. digestion is activated by the presence
b. The time required to digest of:
starch partially by the enzyme- a. chloride ions
salivary amylase. b. iodine
c. The point at which the enzyme c. phosphate ions
shows the maximum activity. d. metals
d. The point at which the enzyme 15. The rate of enzyme-catalyzed
denatured. reaction is influenced by:
11. What is the meant by optimum a. Temperature
temperature for enzyme activity? b. pH
a. The temperature at which the c. Substrate Concentration
enzyme shows the maximum d. Enzyme Concentration
activity. e. All of the above
b. The temperature at which the 16. In the stomach the salivary amylase
enzyme deactivated is not functioning. Why?
c. The temperature at which the a. Digestion of starch is not
enzyme shows the minimum happening in stomach.
activity. b. Stomach is highly acidic.
d. The temperature at which the c. Stomach is highly basic
enzyme denatured. d. pH of stomach is more than 6
12. The experiment set up to study the 17. The salivary amylase hydrolyses
effect of the temperature on the activity starch into _______.
on salivary amylase on starch is carried a. Lipids
out at 10°C. The solution mixture that b. Carbohydrate
contains amylase and starch keeps on c. Fats
giving blue colour for Iodine test about d. Maltose
half an hour. What is the reason for this? 18. The salivary amylase digest starch
a. At the lower temperature, the from food into:
enzyme is deactivated. a. Amylodextrin
b. At lower temperature, reaction b. Erythrodextrin
rate of starch reduces. c. Maltose
d. All of the above
19. Which of the following digestive tryptophan in the intestines, which is a
enzyme is found in the saliva? normal constituent of urine:
a. pepsin a. Uric Acid
b. secretin b. Urea
c. Salivary amylase c. Indican
d. Mucin d. Creatinine
20. This qualitative test on saliva will turn 6. What is the laboratory observation or
it to purple color which indicates the reaction that occurs when urea is
presence of two dipeptide bonds. detected in a urine sample?
a. Achromatic Test a. Formation of a cloudy solution
b. Molisch Test b. Formation of gas
c. Biuret Test c. Formation of a white
d. Millon Test precipitate
d. Foul smell
URINALYSIS
1. A turbid urine could be a cause of: 7. The presence of ketone bodies in the
a. Epithelial cells urine, a condition called ketosis can be
b. High number of bacteria cells detected through this test:
c. Magnesium and Calcium a. Gmelin’s Test
Phosphates b. Rothera’s Test
d. Any of the above c. Benedict’s Test
2. The occurrence of glucose in the d. Modified Benzidine Test
urine is tested through Benedict’s Test. 8. The ff. is/are normal constituents of
When a sample is positive, this condition urine:
is called: a. Urea
a. Nephritis b. Creatinine
b. Glucosuria c. Hippuric Acid
c. Ketosis d. All of the above
d. Diabetes mellitus 9. A urine sample is a little yellowish
3. This is the test to check the presence green to brown in color and when
of protein in the urine sample. shaken foams readily. The urine sample
a. Rothera’s Test could have presence of:
b. Benedict’s Test a. Blood
c. Gmelin’s Test b. Ketone bodies
d. Heller’s ring test c. Uric Acid
4. This compound is the chief end d. Bile
product of purine metabolism in man. 10. The normal odor of a urine is:
a. Uric Acid a. Foul smell
b. Creatinine b. Faint aromatic odor
c. Hippuric Acid c. Sweet smell
d. Indican d. Odorless
5. This compound comes from the 11. The presence of blood in the urine, a
putrefactive decomposition of condition called hemoglobinuria is
tested through:
 a. Modified Benzidine Test c. Starch
b. Rothera’s Test d. Glucose
c. Gmelin’s Test 3. The rate of enzyme catalyze reaction
d. Heller’s Ring Test is influenced by?
12. The presence of these compounds in a. temperature
the urine is due to incomplete oxidation b. pH
of fats: c. substrate concentration
a. Acetone d. all of the above
b. Acetoacetic Acid 4. The point at which a reaction mixture
c. b-hydroxybutyric acid no longer gives a color with iodine
d. All of the above solution. This enables the researcher or
13. What type of urine sample should be student to follow the digestive action of
submitted if a detailed composition of the amylase and enzyme by observing
urine is desired? the time taken to reach this point.
a. Random sample a. Molisch point
b. Early morning urine sample b. Indicator point
c. Midstream urine sample c. End point
d. 24-hour sample d. Achromatic point
14. The following are the factors 5. Which of the following amino acids
affecting the color of urine in the have an indole component in it?
sample: a. Tyrosine
a. Drug intakes b. Arginine
b. Food intakes c. Tryptophan
c. Medical condition d. Phenyl aniline
d. All of the above 6. What type of compound is formed
15. The yellow color of the urine is due when a carboxylic acid reacts with an
to: alcohol in the presence of an acid
a. Melanin catalyst?
b. Urochrome a. Ether
c. Bilirubin b. Ester
d. Urea c. Either a or b, depending on
the condition
REVIEW d. Neither a or b
1. Which of the following would have a 7. What visible evidence can be
positive reaction with iodine test? observed on compounds which have
a. Fructose positive test with Tollen’s Reagent?
b. Starch a. Formation of silver mirror
c. Sucrose b. Formation of brick red
d. Glucose precipitate
2. Which of the following will have a c. Formation of brown precipitate
positive reaction with the Seliwanoff’s d. Formation of gas bubbles
test?
a. Sucrose
b. Lactose
8. Which of the ff. tests is the 14. In the bromine test, if the solution is
characteristic test for the presence of decolorized by the hydrocarbon, then it
phenols in a given sample or can be concluded that the
compound? hydrocarbon is:
a. Iodoform test a. Saturated
b. Lucas’ test b. Unsaturated
c. Ferric Chloride test c. Reactive to light
d. Baeyer test d. None of the above
9. Which of the ff. lipids are considered 15. Which of the ff. compounds will
good emulsifiers? react with sodium hydroxide to give a
a. Lecithin water – soluble salt?
b. Bile Salts a. Alcohols
c. Cholesterol b. Phenols
d. Both a and b c. Carboxylic Acids
10. The following are the factors d. Both b and c
affecting the color of urine in the 16. Which tests are used to distinguish
sample: between aldehydes and ketones?
a. Drug intake a. Tollen’s test
b. Food intake b. Fehling’s test
c. Medical condition c. Benedict’s test
d. All of the above d. All of the above
11. Ninhydrin with evolution of CO2 17. What is the product formed when 10
forms a blue complex with: drops of ethanol is mixed with 4 drops of
a. Peptide bond dilute ethanoic acid and 1 drop of
b. Free Amino acids concentrated sulfuric acid? The mixture
c. Tyrosine is then heated with a low flame in a
d. Tryptophan water bath.
12. Hopkins – Cole test is for a. Methyl ethanoate
identification of: b. Ethyl propanoate
a. Tyrosine c. Ethyl ethanoate
b. Tryptophan d. Propyl ethanoate
c. Arginine 18. This test serves to distinguish between
d. Cysteine monosaccharides and disaccharides
13. The most important natural a. Barfoed’s test
antioxidant is b. Benedict’s test
a. Vitamin D c. Seliwanoff test
b. Vitamin A d. Aniline Acetate test
c. Vitamin E 19. The following are tests for proteins
d. Vitamin K except:
a. Biuret test
b. Millon – Nasse test
c. Molisch test
d. None of the above
20. This is a test to detect the presence 5. Which of the ff. tests is the
of ketone bodies in urine sample characteristic test for the presence of
a. Rothera’s test phenols in a given saturated
b. Heller’s ring test compound?
c. Gmelin’s test a. Lucas Test
d. Benedict’s test b. Baeyer Test
21. The optimum condition for the c. Ferric Chloride Test
salivary amylase is: d. Iodoform Test
a. pH 5.0 6. This is found in the urine sample as a
b. Temperature 5 degC waste product when benzoic acid
c. Temperature 50 degC enters the body and reacts with glycine
d. pH 6.8 (amino acid)
22. The yellow color of the urine is due a. Hippuric Acid
to: b. Uric Acid
a. Urochrome c. Indican
b. Bilirubin d. Creatinine
c. Melanin 7. Which of the ff. factors can cause
d. Urea protein denaturation?
Part II. a. Heating temperatures above
1. Which of the ff. compound/s can turn 50 degC
blue litmus paper to red? b. Addition of acids and bases
a. Ammonia c. Addition of alcohol
b. Trichloroacetic Acid d. All of the above
c. Ethanol 8. The protein found in egg is called?
d. All of the above a. Yolk
2. Which of the ff. compounds has the b. Globulin
most solubility in water? c. Casein
a. Phenol d. Albumin
b. Butanone 9. Which of the ff. tests would allow you
c. 2-Methyl-2-Propanol to differentiate between fructose and
d. Butane sucrose?
3. Oxidation of a secondary alcohol a. Fermentation Test
produces a? b. Seliwanoff Test
a. Aldehyde c. Benedict’s Test
b. Ketone d. Aniline Acetate Test
c. Carboxylic Acid 10. What is the constituent of saliva that
d. Ether makes it slippery?
4. These are compounds that lose an a. Amylase
electron in the chemical reaction b. Mucin
a. Oxidizing agent c. Glycoproteins
b. Reducing agent d. Mineral Salts
c. All of the above
d. None of the above
11. Which of the ff. compounds will give d. Cholesterol
a positive test for Aniline Acetate Test 15. What observations can be seen on
(formation of bright red color in the proteins or amino acid samples that
aniline acetate paper) gives a positive reaction to
a. Glucose Xanthoproteic Test?
b. Fructose a. Formation of intense blue color
c. Xylose compound
d. Sucrose b. Formation of brick red
12. Which of the ff. best describes the precipitate
experimental conditions under which an c. Formation of yellow precipitate
alkane be chlorinated or brominated? d. Formation of violet color
a. Either at room temperatures complex
upon exposure to light or in the 16. Which of the ff. will NOT give a
dark at temperatures exceeding positive reaction with potassium
about 10 degC permanganate test?
b. Only in the dark at a. Acetylene (Ethyne)
temperatures exceeding about b. Hexane
10 degC c. Ethanol
c. Only at room temperature d. Formic Acid (Methanoic Acid)
upon exposure to light 17. What is the enzyme found in saliva
d. In the dark at room that is responsible for the breakdown of
temperature starch in food?
13. Which of the ff. is a pathological a. Erythrodextrin
component of urine? b. Mucin
a. Uric Acid c. Amylase
b. Urea d. Amylodextrin
c. Glucose 18. Which of the ff. is used to define
d. Creatinine lipids?
14. Which of the ff. lipid sample will give a. The presence of specific
a positive reaction with Lieberman- functional group
Burchard test? b. Their chemical structure
a. Lecithin c. Their insolubility in water
b. Tocopherol d. None of these
c. Glycerol
------------------------------------- BioChem LAB -------------------------------------
------------------------------------- BioChem LEC -------------------------------------
Chirality 5. Illustrated here are the four stereoiso
1. If a carbon atom is a stereocenter, mers of an amino acid which has two st
which of the following statements must ereocenters. Which structure(s)
be true about that carbon? is/are diastereomer(s) of structure A?
a. It has four single bonds.
b. It has a double bond and two
single bonds.
c. It has two double bonds. a. B only
d. Any of these bonding arrange b. C only
ments can be associated with a s c. D only
tereocenter. d. B and D only

2. Given the compounds 2,3- Carbohydrates

dimethylpentane and 3- 1. Which of the following is the Fischer pr
methylhexane, which of the following is ojection of D-glyceraldehyde?
true?
a. Only 2,3- a.
dimethylpentane is chiral.
b. Only 3-methylhexane is chiral.
c. Both are chiral.
d. Neither is chiral.
3.In the R,S system, which of the
following groups has highest priority?
a. alkyl 2. How many chiral carbons are present
b. amino in the open-
c. hydroxyl chain form of an aldohexose?
d. thiol a. 3
4. What is the maximum number of stere b. 4
oisomers possible for a molecule which c. 5
has three stereocenters (assuming d. 6
no other structural features that lead to 3. Glycosides are examples of which cla
steroisomerism)? ss of compounds?
a. 2 a. acetals
b. 4 b. hemiacetals
c. 8 c. ethers
d. 16 d. esters
4. What reaction occurs when glucose is
converted to glucitol?
a. reduction at C-1
b. oxidation at C-1
c. reduction at C-6
d. oxidation at C-6
5. Which of the following is true about th 5. Which of the following structural arran
e reducing properties of sugars? gements is the steroid structure?
a. All monosaccharides and all di
a. b.
saccharides are nonreducing sug
ars.
b. All monosaccharides and all di
saccharides are reducing sugars. c. d.
c. All disaccharides and some m
onosaccharides are nonreducing
sugars. 6. Triglycerides belong to which class of
d. All monosaccharides and som compounds?
e disaccharides are reducing sug a. alcohols
ars. b. ether
c. esters
Lipids d. carboxylic acids
1. Which of the following is used to defin 7. Which of the following is true of most fatty
e lipids? acids found in triglycerides?
a. the presence of a specific func a. they are unbranched
tional group b. They contain more than 20 carbon
b. their chemical structure atoms
c. their insolubility in water c. They contain an odd number of
d. none of these carbon atoms
2. Which alcohol is a component of all d. None of the above
triglycerides? 8. Which of the following best describes the
a. 1,2,3-butanetriol components of phospholipids?
b. ethylene glycol a. Glycerol, one fatty acid, two
c. glycerol phosphate groups, and an alcohol
d. none of these b. Glycerol, two fatty acids, a phosphate
3. Which of the following type of reactio group, and an alcohol
n is involved in saponification? c. Either of these, depending on the
a. condensation identity of the phospholipid
b. dehydration d. None of the above
c. hydrogenation 9. Which of the following describes the
d. hydrolysis distribution of the polar and nonpolar portions
4. Which of the following best describes of lipids in the lipid bilayer?
the components of phospholipids? a. Both the inside and outside of the
a. glycerol, one fatty acid, two p bilayer consist of alternating polar
hosphate groups, and an alcohol heads and nonpolar tails
b. glycerol, two fatty acids, a pho b. Both polar heads are on the outside
sphate group, and an alcohol of the bilayer and both hydrophobic
c. either of these, depending on t tails are on the inside of the bilayer
he identity of the phospholipid c. Both polar heads are on the inside of
d. none of the above the bilayer and both hydrophobic tails
are on the outside of the bilayer
 d. Some polar heads are on the inside of Enzymes
the bilayer and some are on the 1. Which of the following is true of
outside; the hydrophobic tails are enzymes?
similarly distributed a. They shift the position of a che
mical equilibrium.
Proteins b. They speed up the rate of a
1. Which functional group is involved in chemical reaction
linking together the amino acids of prot c. both a and b
eins? d. neither a nor b
a. amine 2. The names of enzymes are often
b. anhydride derived from which of the following?
c. carbonyl a. the reaction they catalyze
d. carboxyl b. the compound on which they
2. What is the most important property o act
f the R groups of the amino acids? c. both a and b
a. their polarity d. neither a nor b
b. their shape 3. What name is given to any (organic
c. their size or inorganic) nonprotein portion of an e
d. none of these nzyme?
3. In which of the following do both ami a. apoenzyme
no acids contain a sulfur atom? b. coenzyme
a. cysteine and glycine c. cofactor
b. cysteine and methionine d. substrate
c. histidine and methionine 4. Noncompetitive inhibition can be ofte
d. phenylalanine and tryptophan n explained by which of the following m
4. Which of the following is true about odels?
zwitterions? a. the induced fit model
a. They are positively charged. b. the lock and key model
b. They are negatively charged. c. both a and b
c. They are electrically neutral. d. neither a nor b
d. The charge depends on the zw 5. Which of the following will cause the r
itterion. ate of enzyme activity to increase
5. Which of the following defines the linearly?
primary structure of proteins? a. increasing the enzyme
a. the geometric arrangement of concentration
the backbone atoms b. increasing the substrate
b. the geometric arrangement of concentration
the side chains c. increasing the temperature
c. the sequence of the amino d. increasing the pH
acids
d. all of the above
Nucleotides, Nucleic Acids & Heredity Gene Expression and Protein Synthesis
1. A single gene will always do which of 1. A sequence of how many bases is ne
the following? eded to code for an amino acid?
a. determine a physical characte a. 1
ristic of an individual b. 2
b. code for a specific protein or t c. 3
ype of RNA d. 4
c. both a and b 2. Which of the following is involved in u
d. neither a nor b nwinding the DNA double helix?
2. Which of the following is not a compo a. acetylase
nent of a nucleotide? b. helicase
a. a base c. binding proteins
b. a disaccharide d. polymerases
c. a monosaccharide 3. Which of the following is true during
d. a phosphate transcription?
3. Which base found in RNA is not found a. Both strands of DNA serve as a
in DNA? template for RNA.
a. adenine b. Only the antisense strand of D
b. cytosine NA
c. guanine serves as a template for RNA.
d. uracil c. Only the sense strand of DNA s
4. Which of the following is not part of th erves
e backbone of DNA? as a template for RNA.
a. a bases d. Transcription involves merging
b. the phosphates information from both strands of
c. the sugars DNA.
d. None, they are all part of the b 4. Which base will be added to the gro
ackbone. wing mRNA if the base on the DNA tem
5. Which pair of bases is linked together plate is adenine?
by three hydrogen bonds? a. cytosine
a. adenine and cytosine b. guanine
b. adenine and thymine c. thymine
c. guanine and cytosine d. uracil
d. guanine and thymine 5. The genetic code is associated with w
6. Why is replication called semiconserv hich of the following processes?
ative? a. DNA replication
a. Only some of the leading stran b. translation
d is conserved. c. transcription
b. Only some of the lagging stan d. all of these
d is conserved.
c. Each new molecule of DNA co
ntains one template stand and o
ne daughter strand.
d. All of the above are correct.
Bioenergetics REVIEW
1. Which part of metabolism involves the
If the bond angles at a carbon atom are
synthesis of molecules? 109.5°, how many groups are distributed
a. anabolism around the carbon atom?
b. catabolism
c. both a and b a) 1
d. neither a and b b) 2
2. Mitochondria have how many
membranes? c) 3
a. 0 d) 4
b. 1
c. 2 Which of the following functional groups
does not contain a carbonyl group?
d. 4
3. The NAD+/NAD and FAD/FADH2 a) Ester
systems
transport which of the following? b) Alcohol
a. electrons c) Aldehyde
b. hydrogen ions
c. both a and b d) Ketone
d. neither a nor b What is the correct IUPAC name for the
4. How many molecules of CO2 are following compound?
produced by one pass through the citric
acid a) 2,2-dimethyl-4-pentene
cycle? b) 4,4-dimethyl-2-pentene
a. 1
b. 2 c) 4,4-dimethyl-1-pentene
c. 3 d) isoheptene
d. 4
5. Where in the mitochondrion does the Markovnikov's rule is not useful for predicting
the outcome of the reaction of hydrogen
actual conversion of ADP to ATP occur?
chloride with which of the following
a. in the intermembrane space alkenes?
b. in the matrix
c. on the inner membrane a) 1-butene
d. on the outer membrane b) 2-butene
6. How many molecules of O2
are consumed for each C2 c) 2-methylpropene
fragment which enters the
d) 1-pentene
common catabolic pathway?
a. 1
b. 2
c. 3
d. 4
What is the IUPAC name of the following Which of the following compounds contain
compound? a carboxylic acid functional group?

a) methyl propyl methanol

b) 2-pentanol a. A and B only

c) 1-methyl-1-butanol b. A and C only

d) 4-pentanol c. C only

In which class of compounds is the carbon d. B only

at the functional group most highly
oxidized? What is the product obtained when a
carboxylic acid is reduced?
a) Alcohols
a) an alkene
b) Aldehydes
b) a primary alcohol
c) Carboxylic acids
c) a secondary alcohol
d) Ketones
d) a ketone
Which functional group is found in
aldehydes? What is the IUPAC name for the following
compound?
a) −CHO

b) −CH2OH

c) −COOH
a) propyl ethanoate
d) RCOR'
b) butyl ethanoate
Which of the following will give a positive
test with Tollens' reagent? c) ethyl propanoate

a) Aldehydes d) ethyl butanoate

b) Ketones Which of the following is commonly used as

the catalyst in a Fischer esterification?
c) Both a and b
a) acetic acid
d) Neither a nor b
b) dilute hydrochloric acid

c) dilute sulfuric acid

d) concentrated sulfuric acid