ESCO CO2 Incubator CCL-User Manual-V.u-feb2021

Thank you for purchasing this Esco CO2 Incubator.
Please read this manual thoroughly to familiarize

yourself with the many unique features and
exciting innovations we have built into your new
equipment.
Esco provides many other resources at

www.escoglobal.com, to complement this
manual and help you enjoy many years of
productive and safe use of Esco Products.
For Technical Service, contact

NORTH AMERICA
Esco Technologies, Inc.
903 Sheehy Drive, Suite F, Horsham, PA 19044, USA
Tel 215-441-9661 • Fax 484-698-7757
www.escolifesciences.us • eti.admin@escoglobal.com
REST OF THE WORLD

Esco Micro Pte. Ltd.
21 Changi South Street 1 • Singapore 486 777
Tel +65 6542 0833 • Fax +65 6542 6920
www.escoglobal.com • mail@escoglobal.com
User Manual
Esco CelCulture® CO2 Incubator User Manual
CelCulture®
Version U- Released February 2021 CO2 Incubators
Copyright Information
© Copyright 2021 Esco Micro Pte. Ltd. All rights reserved.
The information contained in this manual and the accompanying product is copyrighted and all rights are
reserved by Esco.
Esco reserves the right to make periodic minor design changes without obligation to notify any
person or entity of such change.
CelCulture® is a registered trademark of Esco.
“Material in this manual is provided for informational purposes only. The contents and the product described
in this manual (including any appendix, addendum, attachment or inclusion), are subject to change without
notice. Esco makes no representations or warranties as to the accuracy of the information contained in this
manual. In no event shall Esco be held liable for any damages, direct or consequential, arising out of or related
to the use of this manual.”
i
Table of Contents
Introductory Page
i Table of Contents
iii Manual Revision History
vi Warranty Terms and Conditions
viii Introduction
viii 1. Products Covered
x 2. Safety Warning
x 3. Limitation of Liability
xi 4. European Union Directives on WEEE and RoHS
xi 5. Symbols
xii Declaration of Conformity
1 Chapter 1 – Product Information

1 1.1 About CelCulture® CO₂ Incubator
1 1.2 Labels
2 1.3 Quick View
2 1.3.1 Front Quick View (CCL-050_-_-_)
3 1.3.2 Front Quick View (CCL-170_-_-_, CCL-240_-_-_)
4 1.3.3 Front Quick View (CCL-170_-_-UV, CCL-170_-_-P-UV, CCL-240_-_-UV,
CCL-240_-_-P-UV)
5 1.3.4 Back Quick View (CCL-050_-_-_, CCL-170_-_-_, CCL-240_-_-_, CCL-170_-_-
UV, CCL-240_-_-UV)
6 1.3.5 Back Quick View (CCL-170_-_-P, CCL-240_-_-P, CCL-170_-_-P-UV, CCL-
240_-_-P-UV)
7 Chapter 2 – Installation
7 2.1 General Requirements
7 2.1.1 Location Requirements
8 2.1.2 Environmental Requirements
8 2.1.3 Power Requirements
9 2.2 Installation
9 2.2.1 Unit Leveling
9 2.2.2 Unit Stacking
9 2.2.3 ULPA Filter Installation
11 2.2.4 Shelves System Installation
11 2.2.5 Connecting the Unit to Electrical Power
11 2.2.6 Connecting the Unit to CO₂ and N2 Gas Supply
12 2.2.7 Connecting Alarm Contacts and RS485 Communication Port
12 2.3 Preliminary Cleaning
13 2.4 Filling the Humidity Pan
13 2.5 Filtered Air Exchange
14 2.6 Use of Additional Devices in the CO₂ Incubator
14 2.7 First Run
15 2.8 UV Lamp Functionality
15 2.9 Replacing Old CO₂ Sensor to the Latest IR Sensor
15 2.10 Disclaimer
16 Chapter 3 – Control System and Operation

16 3.1 CelCulture® Control System
18 3.2 Menu Options
19 3.2.1 Settings
19 3.2.2 Calibration
22 3.2.3 Admin
25 3.2.4 Data Logging
CO2 Incubator
ii
25 3.2.5 Service
26 3.2.6 Help
26 3.3 Operation of CO₂ Incubator with Peltier Cooling System
26 3.3.1 Cooling Mode
26 3.3.2 Heating Mode
27 3.4 Diagnostic Readout
28 3.5 Alarm
29 Chapter 4 – General Maintenance

29 4.1 Scheduled Maintenance
29 4.1.1 Check CO₂/N2 Gas Tank Level
29 4.1.2 Check Water Level in the Humidity Pan
29 4.1.3 Cleaning and Decontamination
29 4.1.4 General Inspection
29 4.1.5 Calibration of Temperature, CO₂, O2 and Humidity
29 4.1.6 Change ULPA Filter
30 4.1.7 Change Inlet Filter
30 4.1.8 Change Outer Door Magnetic Gasket
30 4.1.8 Change UV Lamp
30 4.2 Maintenance/Service Log
31 Chapter 5 – Cleaning and Decontamination

31 5.1 Cleaning Procedure
33 5.2 Decontamination
33 5.2.1 Decontamination Procedure
35 Chapter 6 – Troubleshooting
40 Chapter 7 – Technical Specifications

40 Standard CelCulture® CO₂ Incubators (CCL-050_-_-_, CCL-170_-_-_, CCL-240_-_-_)
42 Appendix
43 Appendix A: Log Record
CelCulture®
iii
MANUAL REVISION HISTORY
SERIAL # OF 1 STUNIT
REV. NO. DATE RELEASED DESCRIPTION OF CHANGE REFERENCE FOR
IMPLEMENTATION
Add in CCL-50L and CCL-240L volume
D 12 June 2014 - -
size
Remove A series and upgrade CLM-A
E 8 January 2016 ECN-01-CO₂-2016 2016-106827
to CLM-B
F 12 July 2016 Update cooling incubator operational ECN-05-CO₂-2016 2016-110932
Disclaimer: CCL-050 unit has no
G 17 March 2017 drying function in its - -
decontamination cycle
H 10 May 2017 Add in CCL-UV model - -
Add in troubleshooting instructions - 2015-97750
for outer door alignment and use of
stainless steel hinge
7 September 2017 Add in instructions for changing to - 2015-97017
latest ULPA filter design
I Add in instructions for changing to - 2016-114541
latest IR CO 2 sensor
04 October 2017 Updated warranty policy - -
Updated preliminary cleaning - -
06 November 2017
instructions
18 November 2017 Updated device label - -
13 March 2018 Changed water recommendation - -
Updated directives for low voltage - -
16 March 2018
and electromagnetic compatibility
J
Add in information on compliance to - -
20 June 2018 RoHS in Electrical and Electronic
Equipment directives
Added use of filter adaptor for -
upgrading to current ULPA filter STB-CO2-25
K 27 July 2018 design configuration
Updated parameter recovery time for - -
standard models
Updated minimum height clearance - -
of unit from floor
Updated ambient temperature and - -
24 August 2018 humidity requirements
Updated condition for removal of - -
L
water from humidity pan in model
with Peltier cooling system
Updated the minimum distance of - -
27 August 2018 incubator from air conditioner or
other moving current
Change O₂ sensor to Zirconium
09 November 2018 ECN-CO2-18-003 2018-131940
dioxide
M Additional instruction for when a - -
heat-generating device is to be added
in the incubator
CO2 Incubator
iv
Updated specifications for - -

suppressed O₂ control
16 January 2019
Updated instructions for proper - -
N removal of IR CO₂ sensor
Updated general maintenance - -
11 March 2019 schedule for cleaning and changing of
magnetic gasket
Updated technical specifications on ECN-CO2-2018-
O 16 April 2019 -
page 42 004
Added disclaimer for temperature
uniformity and accuracy, CO 2 and O2
- -
accuracy in the technical specification
table on page 42
Added initial value of 98% on
Temperature, CO 2, O 2 Recovery Time
- -
in the technical specification table.
2 August 2019
On page 42
Added the ff. part numbers (5170989, STB-CO2-25
P
5170990, 5170992) required to Revision B
upgrade to latest filter design in the
-
Installing ULPA Filter from old to
latest configuration part (2.2.3.1) on
page 9
Updated product warranty list and -
Technical Support, Warranty Service
16 October 2019 -
Contacts on the Warranty terms and
conditions part.
Added Instructions for Proper -
18 January 2020 Maintenance of Infrared (IR) GMP251 -
Q
CO2 Sensor page 35.
19 March 2020 Updated Declaration of Conformity - -
Added Backup Running and Check -
CO2 System on the alarm system part -
on page 29.
R 24 May 2020
Updated troubleshooting part -
(updated problem 3 on page 35 and -
added problem 21 on page 40).
Revised resetting of O 2 sensor for 50L -
-
and 170/240L on page 20 and 21.
Enhanced alarm description for
Backup Running and Check CO 2
System on the alarm system on page
S 19 August 2020
29.
Updated disclaimer on technical
specification table from “temperature
not exceeding 37°C” to “temperature
not exceeding 37.2°C” on page 43.
Updated decontamination procedure
on page 33 and indicated that IR STB-2020-CO2-
sensor should not be removed during 003
T 22 November 2020
decon procedure.
Removed Instructions for Infrared (IR)
GMP251 CO 2 Sensor Removal
CelCulture®
v
Removed Instructions for Proper

Maintenance of Infrared (IR) GMP251
CO2 Sensor
Updated settings on set %CO 2.
Changed %CO 2 range from 0%-20% to
0%-19.5% on page 19.
Updated settings on set %O 2. Changed
%O2 range from 1%-20.7% to 1%-18%
on page 19.
Updated unit calibration for 50L,
170L, and 240L units on O 2% value on
page 20 and 21.
Updated technical specification.
Changed %CO 2 range from 0%-20% to
0%-19.5% on page 40.
Changed %O 2 range from 1%-20.7%
to 1%-18% on page 40.
Changed relative humidity value from
up to 95% (standard unit) and up to
91% (suppressed O 2 model) to 85%-
90% on page 40.
Updated CE Declaration of
Conformity
Updated Low %RH Alarm value from
90% to 75% on page 23
Updated unit’s spacing from the floor
value from ≥212 mm to ≥148 mm on
page 7 and 8.
U 11 February 2021 Updated unit stacking description on
page 9.
Updated alarm table and changed
alarm delay values for High CO 2 & O 2
Levels and Low Temperature, CO 2 &
O2 Levels to 15 minutes on page 28.
Updated Unit Calibration instruction
on page 19.
CO2 Incubator
vi
Warranty Terms and Conditions
Esco products come with a limited warranty. The warranty period will vary depending on the product purchased,
beginning on the date of shipment from any Esco international warehousing location. To determine which
warranty applies to your product, refer to the appendix below.
Esco's limited warranty covers defects in materials and workmanship. Esco's liability under this limited warranty
shall be, at our option, to repair or replace any defective parts of the equipment, provided if proven to the
satisfaction of Esco that these parts were defective at the time of being sold, and that all defective parts shall be
returned, properly identified with a Return Authorization.
This limited warranty covers parts only, and not transportation/insurance charges.
This limited warranty does not cover:

• Freight or installation (inside delivery handling) damage. If your product was damaged in transit, you
must file a claim directly with the freight carrier.
• Products with missing or defaced serial numbers.
• Products for which Esco has not received payment.
• Problems that result from:
o External causes such as accident, abuse, misuse, problems with electrical power, improper
operating environmental conditions.
o Servicing not authorized by Esco.
o Usage that is not in accordance with product instructions.
o Failure to follow the product instructions.
o Failure to perform preventive maintenance.
o Problems caused by using accessories, parts, or components not supplied by Esco.
o Damage by fire, floods, or acts of God.
o Customer modifications to the product
• Consumables such as filters (HEPA, ULPA, carbon, pre-filters) and fluorescent / UV bulbs.
• Esco is not liable for any damage incurred on the objects used on or stored in Esco equipment. If the
objects are highly valuable, the use of a completely independent backup system and a 24-hr
redundant monitoring system with alarm capability are recommended.
Factory installed, customer specified equipment or accessories are warranted only to the extent guaranteed by
the original manufacturer. The customer agrees that in relation to these products purchased through Esco, our
limited warranty shall not apply and the original manufacturer's warranty shall be the sole warranty in respect
of these products. The customer shall utilize that warranty for the support of such products and in any event
not look to Esco for such warranty support.
Esco encourages all users to register their equipment online at www.escoglobal.com/warranty_registrations.

php or complete the warranty registration form included with each product.
ALL EXPRESS AND IMPLIED WARRANTIES FOR THE PRODUCT, INCLUDING BUT NOT LIMITED TO ANY IMPLIED
WARRANTIES AND CONDITIONS OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE LIMITED
IN TIME TO THE TERM OF THIS LIMITED WARRANTY. NO WARRANTIES, WHETHER EXPRESS OR IMPLIED, WILL
APPLY AFTER THE LIMITED WARRANTY PERIOD HAS EXPIRED. ESCO DOES NOT ACCEPT LIABILITY BEYOND THE
REMEDIES PROVIDED FOR IN THIS LIMITED WARRANTY OR FOR SPECIAL, INDIRECT, CONSEQUENTIAL OR
INCIDENTAL DAMAGES, INCLUDING, WITHOUT LIMITATION, ANY LIABILITY FOR THIRD-PARTY CLAIMS AGAINST
YOU FOR DAMAGES, FOR PRODUCTS NOT BEING AVAILABLE FOR USE, OR FOR LOST WORK. ESCO'S LIABILITY
WILL BE NO MORE THAN THE AMOUNT YOU PAID FOR THE PRODUCT THAT IS THE SUBJECT OF A CLAIM. THIS IS
THE MAXIMUM AMOUNT FOR WHICH ESCO IS RESPONSIBLE.
CelCulture®
vii
These Terms and Conditions shall be governed by and construed in accordance with the laws of Singapore and
shall be subject to the exclusive jurisdiction of the courts of Singapore.
Technical Support, Warranty Service Contacts

USA: 1 215-441-9661
Singapore: +65 6542 0833
For more information, visit http://www.escoglobal.com/.
Distributors are encouraged to visit www.escoglobal.net for additional materials.
Product Appendix, Warranty Listing
CO2 Incubators 2 years limited
Note: The warranty periods may vary by country. Contact your local distributor for specific warranty details.
For international distributors, warranty period starts two months from the date the equipment is shipped from
Esco facility. This allows shipping time so the warranty will go into effect at approximately the same time the
equipment is delivered to the user. The warranty protection extends to any subsequent owner during the
warranty period. Distributors who stock Esco equipment are allowed an additional four months for delivery and
installation, providing the product is registered with Esco. User can register their products online at
www.escoglobal.com/warranty or complete the warranty registration form include with each product.
Policy updated on 1st January 2015 (This limited warranty policy applies to products purchased on and after 1 st
January 2015)
CO2 Incubator
viii
Introduction
1. Products Covered
CelCulture CO₂ Incubator (Stainless Steel Chamber with Curved Door)
Model Size Sensor Control Filter Decontamination Electrical Rating
CCL-050B-8 50 L IR CO₂ N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050B-8-SS 50 L IR CO₂ N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050B-9 50 L IR CO₂ N/A 90oC Moist Heat 110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-050T-8 50 L IR CO₂/O2 N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050T-8-SS 50 L IR CO₂/O2 N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050T-9 50 L IR CO₂/O2 N/A 90oC Moist Heat 110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-170B-8 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170B-8-NF 170 L IR CO₂ N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170B-8-SS 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170B-9-SS 170 L IR CO₂ ULPA 90oC Moist Heat 110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-170T-8 170 L IR CO₂/O2 ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170T-8-NF 170 L IR CO₂/O2 N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CelCulture CO₂ Incubator (Stainless Steel Chamber with Flat Door)

CCL-050B-8-FD 50 L IR CO₂ N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050B-9-FD 50 L IR CO₂ N/A 90oC Moist Heat 110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-050T-8-FD 50 L IR CO₂/O2 N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050T-9-FD 50 L IR CO₂/O2 N/A 90oC Moist Heat 110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-170B-8-FD 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170T-8-FD 170 L IR CO₂/O2 ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CelCulture CO₂ Incubator (for IVF Application)

CCL-050B-8-IVF 50 L IR CO₂ N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050B-9-IVF 50 L IR CO₂ N/A 90oC Moist Heat 110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-050T-8-IVF 50 L IR CO₂/O2 N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CelCulture®
ix
CCL-050T-9-IVF 50 L IR CO₂/O2 N/A 90oC Moist Heat 110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-170B-8-IVF 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170T-8-IVF 170 L IR CO₂/O2 ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CelCulture CO₂ Incubator (100% Copper Chamber)

CCL-050B-8-Cu 50 L IR CO₂ N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050B-9-Cu 50 L IR CO₂ N/A 90oC Moist Heat 110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-170B-8-Cu 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050T-8-Cu 50 L IR CO₂/O2 N/A 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-050T-9-Cu 50 L IR CO₂/O2 N/A 90oC Moist Heat 110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-170T-8-Cu 170 L IR CO₂/O2 ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CelCulture CO₂ Incubator (with Peltier Cooling Mode)

CCL-170B-8-P 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170T-8-P 170 L IR CO₂/O2 ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CelCulture CO₂ Incubator with UV Lamp (Stainless Steel Chamber with Curved Door)
CCL-170B-8-UV 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170T-8-UV 170 L IR CO₂/O2 ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CelCulture CO₂ Incubator with UV Lamp (Stainless Steel Chamber with Flat Door)
CCL-170B-8-FD-UV 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170T-8-FD-UV 170 L IR CO₂/O2 ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CO2 Incubator
x
CelCulture CO₂ Incubator with UV Lamp (100% Copper Chamber)

CCL-170B-8-Cu-UV 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170T-8-Cu-UV 170 L IR CO₂/O2 ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CelCulture CO₂ Incubator with UV Lamp (with Peltier Cooling Mode)

CCL-170B-8-P-UV 170 L IR CO₂ ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170T-8-P-UV 170 L IR CO₂/O2 ULPA 90oC Moist Heat 220 - 240 V, AC, 50/60 Hz, 1Φ
2. Safety Warning
• Anyone working with, on or around this equipment should read this manual. Failure to read,
understand and follow the instructions given in this documentation may result in damage to the unit,
injury to operating personnel and/or poor equipment performance.
• Any adjustment, modification or maintenance to this equipment must be undertaken by qualified

service personnel.
• The use of any hazardous materials in this equipment must be monitored by an industrial hygienist,
safety officer or some other suitably qualified individual.
• Before you proceed, you should thoroughly understand the installation procedures and take note of
the environmental / electrical requirements.
• In this manual, important safety related points will be marked with the symbol .
• If the equipment is used in a manner not specified by this manual, the protection provided by this
equipment may be impaired.
3. Limitation of Liability
• The disposal and / or emission of substances used in connection with this equipment may be governed
by various local regulations. Familiarization and compliance with any such regulations are the sole
responsibility of the users. Esco’s liability is limited with respect to user compliance with such
regulations.
CelCulture®
xi
• Esco Voyager® is a PC-based software package developed for the remote monitoring, data logging, and
programming / device configuration of some models of Esco controlled environment laboratory
equipment.
If customers want to use their own PC-based software to control Esco equipment, Esco may, at its sole
discretion, after a non-disclosure agreement is executed, provide the Esco communication protocol to
the customer. Under no circumstances shall Esco be responsible for integration work, or providing
additional technical support, unless a special agreement is entered into separately.
4. European Union Directive on WEEE and RoHS

The European Union has issued two directives:
• Directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE)
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:
Esco sells products through distributors throughout Europe. Contact your local Esco distributor
for recycling/disposal.
Recommended method of disposal is according to The Federal, State and Local Governmental
regulations.
• Directive 2002/95/EC on Restriction on the use of Hazardous Substances (RoHS)

With respect to the directive on RoHS, please note that this CO₂ incubator falls under category 8 (medical
devices) and category 9 (monitoring and control instruments) and is therefore exempted from requirement to
comply with the provisions of this directive.
5. Symbols
The information and instructions in this manual may be accompanied by symbols shown in the table below. They
draw the reader's attention to important information on the use, safety and maintenance of incubator.
Caution: Electrical Hazard

Lift this panel to service the electrical, filter and blower
systems. Disconnect power before electrical service.
Caution: Hot Surface

Hot surfaces inside. Avoid contact.
CO2 Incubator
xii
Declaration of Conformity
In accordance to EN ISO/IEC 17050-1:2010
We, Esco Micro Pte.Ltd.

of 21 Changi South Street 1
Singapore, 486777
Tel: +65 6542 0833
Fax: +65 6542 6920
declare on our sole responsibility that the product:

Category : CO₂ Incubator
Brand : CelCulture
Model : CCL-050B-_, CCL-050B-_-SS, CCL-050T-_, CCL-050T-_-SS, CCL-170B-_, CCL-170B-_-
NF, CCL-170B-_-SS, CCL-170T-_, CCL-170T-_-NF, CCL-170T-_-SS, CCL-240B-_,CCL-
240B-_-NF, CCL-240B-_-SS, CCL-240T-_, CCL-240T-_-NF, CCL-240T-_-SS, CCL-050B-_-
FD, CCL-050T-_-FD, CCL-170B-_-FD, CCL-170T-_-FD, CCL-240B-_-FD, CCL-240T-_-FD,
CCL-050B-_-IVF, CCL-050T-_-IVF, CCL-170B-_-IVF, CCL-170T-_-IVF, CCL-240B-_-IVF,
CCL-240T-_-IVF, CCL-050B-_-Cu, CCL-170B-_-Cu, CCL-240B-_-Cu, CCL-050T-_-Cu, CCL-
170T-_-Cu, CCL-240T-_-Cu, CCL-170B-_-P, CCL-170T-_-P, CCL-240B-_-P, CCL-240T-_-
P, CCL-170B-_-UV, CCL-170T-_-UV, CCL-240B-_-UV, CCL-240T-_-UV, CCL-170B-_-FD-
UV, CCL-170T-_-FD-UV, CCL-240B-_-FD-UV, CCL-240T-_-FD-UV, CCL-170B-_-Cu-UV,
CCL-240B-_-Cu-UV, CCL-170T-_-Cu-UV, CCL-240T-_-Cu-UV, CCL-170B-_-P-UV, CCL-
170T-_-P-UV, CCL-240B-_-P-UV, CCL-240T-_-P-UV
in accordance with the following directives:

2014/35/EU : The Low Voltage Directive and its amending directives
2014/30/EU : The Electromagnetic Compatibility Directive and its amending directives
2011/65/EU : The RoHS in Electrical and Electronic Equipment Directive and its amending
directives
has been certified independently to comply with the requirement of the following Harmonized Standard:
Low Voltage : EN 61010-1:2010
EMC : EN 61326-1:2013
More information may be obtained from Esco’s authorized distributors located within the European Union. A
list of these parties and their contact information is available on request from Esco.
____________________
Lim Lay Yew
Director, Esco
CelCulture®
1
Chapter 1 - Product Information

1.1 About CelCulture® CO₂ Incubators
A CO₂ incubator is a device designed to copy the cells’ natural environment by controlling physical parameters
such as the temperature, humidity, CO₂ and O₂ levels for the optimum growth and development of cells. It is
essential for experimental work in cell biology, microbiology and molecular biology, tissue engineering, in vitro
fertilization, neuroscience, cancer research and other cell research. It is widely used in mammalian cell research
with a setting of 37°C, 5% CO₂ concentration and 80-95% humidity.
Esco CelCulture® CO₂ Incubator is designed for high-quality incubation performance through the precise control
of heating and gas injections combined with multiple contamination control and ergonomic features appropriate
for safe incubation of many types of cells, tissues, and similar.
The external body of CelCulture® is electro-galvanized steel coated with silver-ion impregnated ISOCIDE™
antimicrobial powder coating which kills 99.9% of surface contaminants within 24 hours of exposure. The
incubator chamber is stainless steel type 304 with rounded corners for easy cleaning. Its perforated shelves,
shelves system, plenums and humidity pan are also made of shiny stainless steel. Interior components are easily
removed without using any tools. The inner door with tight gasket seal is heated to prevent condensation.
Heater foils directly mounted on the outer surfaces of the inner chamber helps maintain a relatively uniform
temperature with fast recovery time after door opening. A high-density insulation completely protects the
heating elements. It is then surrounded by an air jacket which separates the chamber from the varying ambient
parameters. The unit is equipped with a drift-free, non-dispersive infrared sensor which has temperature and
pressure compensating feature for longer-lasting reliability. For models with suppressed O₂ control, the unit is
equipped with a highly accurate zirconia O₂ sensor with non-depleting technology for longer lasting reliability.
Forced convection design accelerates humidification via humidity pan to prevent desiccation of cell samples. An
ULPA filtration system, which is 99.999% efficient at 0.1 µ to 0.2 µ particulates, is connected to the blower to
ensure proper circulation of an ISO Class 5 clean air standard without affecting cell growth negatively. It is
attached to a blower fan to ensure proper circulation of clean air and equal distribution of heat and gas at any
point in the incubator chamber.
Given the precise measurement and control of temperature and CO₂ level, as well as the multiple contamination
control methods of CelCulture® CO₂ incubator, it is especially suitable for growing mammalian cells at 37°C and
in the presence of 5% CO₂.
1.2 Labels
Model – model of the unit

Serial – the unit’s serial number
Power – max power requirement and electrical specification
Interior Volume – the unit’s inner chamber volume
Manufactured in ____ – year of manufacture
CO2 Incubator
2
1.3 Quick View

1.3.1 Front Quick View (CCL-050_-_-_)
10
11
1. Electrical panel cover

2. Door switch
3. Shelves
4. Inner glass door*
5. Inner glass door latch
6. Shelving bracket
7. Shelving rails
8. Outer door
9. Leveling feet
10. Inner glass door gasket
11. Magnetic block
*CCL-050_-_-IVF units have 2 sealed inner glass doors
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3
1.3.2 Front Quick View (CCL-170_-_-_, CCL-240_-_-_)
16

2. Door switch
3. Top plenum with circulation fan
4. ULPA filter
5. Shelf
6. Sampling port
8. Humidity pan
9. Leveling feet
10. Control panel
11. Outer door
12. Inner glass door*
13. Pilaster shelf support
15. Magnetic gasket
16. Magnetic block
*CCL-170_-_-IVF units have 4 sealed inner glass doors

CCL-240_-_-IVF units have 4 or 6 sealed inner glass doors
CO2 Incubator
4
1.3.3 Front Quick View (CCL-170_-_-UV, CCL-170_-_-P-UV, CCL-240_-_-UV, CCL-240_-_-P-UV)
11
12
13
14
15
8
16
9
10
17

2. Door switch
3. Top plenum with circulation fan
4. ULPA filter
5. Shelf
7. Sampling port
8. UV Lamp (for models with UV)
9. Humidity pan
10. Leveling feet
11. Control panel
12. Outer door
13. Inner glass door
14. Pilaster shelf support
16. Magnetic gasket
17. Magnetic block
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5
1.3.4 Back Quick View (CCL-050_-_-_, CCL-170_-_-_, CCL-240_-_-_, CCL-170_-_-UV, CCL-240_-

_-UV)
1
7
2
8
9
3
10
4
11
5
1. Power switch
2. Cooling fan*
3. Power inlet
4. RS485 communication port
5. Analog output (optional)
6. Alarm contacts
7. CO₂ gas inlet #1
8. CO₂ gas inlet #2 (for optional CO₂ backup system)
9. N2 gas inlet #1 (for suppressed O2 control units)
10. N2 gas inlet #2 (for optional N2 backup system in units
with suppressed O2 control)
11. Access ports**
*not present in 50L models

**only 1 access port for 50L models
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6
1.3.5 Back Quick View (CCL-170_-_-P, CCL-240_-_-P, CCL-170_-_-P-UV, CCL-240_-_-P-UV)
1
7
2
8
9
3
10
4
11
5
12
1. Power switch
2. Cooling fan
3. Power inlet
4. RS485 communication port
5. Analog output (optional)
6. Alarm contacts
7. CO₂ gas inlet #1
8. CO₂ gas inlet #2 (for optional CO₂ backup system)
9. N2 gas inlet #1 (for suppressed O2 control units)
10. N2 gas inlet #2 (for optional N2 backup system in units
with suppressed O2 control)
11. Access port
12. Peltier cooling module
CelCulture®
7
Chapter 2 – Installation
2.1 General Requirements
2.1.1 Location Requirements

• The CelCulture® CO₂ Incubator should be placed on a dry, stable and sturdy working surface or on the
various optionally available floor stands. It should NOT be placed directly on the floor in order to
prevent condensation and the risk of contamination.
• Never place the unit close to heating or cooling ducts, flammable materials or devices that produce
excess heat such as autoclaves, radiators, ovens, and similar. Do NOT install equipment outdoors, near
windows, doors and other areas with rapidly moving air currents and/or direct sunlight. Installation in
improper locations will greatly affect the equipment’s performance.
• Before moving or relocating the unit, make sure to:
o remove the water in the humidity pan to avoid spillage;
o transfer the sample cells to another incubator; and
o decontaminate the equipment.
• It is essential to ensure adequate air ventilation around the equipment to allow proper heat and gas
dissipation, but do not place the unit directly in the path of moving air currents. It is required to allow
at least 2000 mm distance between the incubator and the air conditioner or any moving current.
≥148
• The spacing between the unit and the side wall should be at least 100 mm (3.9”).
• The spacing at the back of the equipment should be at least 110 mm (4.3”) to allow connections to the
rear panel of the equipment.
CO2 Incubator
8
• The spacing from the floor must be ≥148 mm (5.8”). Never place equipment directly on the floor. It is
recommended to use the optionally available floor stand to elevate the unit from the floor.
2.1.1.1 Relocating the Unit
Normally, units are rarely moved once they are in their ideal positions, but should there be a
need to relocate or repackage the unit, here are some considerations:
• It is recommended that risk assessment is carried out before the unit is moved.
• Before moving the unit, remember to decontaminate the incubator.
• Before moving the unit, remember to secure all moving parts (e.g. glass door) and remove
the water in the humidity pan to avoid spillage.
• Make sure to transfer all sample cells to another incubator.
• Unit is heavy so please carry out adequate workplace safety assessment before moving
the incubator. Do not lift using the door or door handle.
• Should only manpower is available (i.e. no suitable equipment), it will usually take 6 or
more people to move a unit manually.
• In relocating the unit through an elevated surface, ensure that the unit is held securely,
and should face the ramp side up, as shown below:
For repackaging:
• Place the unit on the pallet.
• Strap the unit body down to the pallet.
• Repackage as necessary. If possible, use original packaging.
• When moving the unit, use material handling equipment and lift the pallet.
2.1.2 Environmental Requirements
Location Indoor use only

Altitude limit 2000 m (6600 ft.)
Ambient humidity 30% - 75% (non-condensing)
Note: For optimum performance, room humidity must be 60%.
Ambient temperature 18°C - 30°C (64.4°F – 86.0°F)
Note: For optimum performance, room temperature must be constantly at 20°C - 24°C
(68°F – 75°F). If ambient temperature is not in the range of 20°C - 24°C, the temperature
of the unit must be calibrated.
Ventilation ZH 1/119 (Guidelines for laboratories)
Overvoltage category II in accordance to IEC 61010-1
Pollution degree 2.0
Note: In pollution degree 2.0, it is assumed that only non-conductive pollutants such as
dust are present, except when occasional conductivity caused by condensation.
2.1.3 Power Requirements

• The equipment is designed to work with an electrical supply of 220 VAC – 240 VAC, 50/60 Hz or 110
VAC – 130 VAC, 50/60 Hz. If your available electrical supply is not within these parameters, a suitable
CelCulture®
9
power supply must be used, otherwise damage may be caused to the unit or a hazardous situation may
occur.
• It is recommended that the equipment is connected to a dedicated power source with protective
grounding installed.
• There should be unobstructed access to the main power plug. The power plug is the main disconnecting
device on the unit.
• This equipment can sustain a maximum of ±10% nominal voltage fluctuation; otherwise a power
stabilizer is needed.
• The cable length must be shorter than 3 meters.
2.2 Installation
2.2.1 Unit Leveling

It is important that the equipment is properly leveled by positioning a bubble level on the center top shelf of the
incubator. The incubator has a built-in leveling feet that can be adjusted to level the unit. Adjust the feet until
the unit sits level left to right and front to back.
2.2.2 Unit Stacking

If the equipment is to be stacked on top
of each other, it is advisable to use a
floor stand with leveling feet or a two
units floor stand, and the unit with the
lower working temperature must always
be placed at the bottom.
1. Remove the screws from the
back at top left and top right of
the lower incubator. These
screws will be used to secure
the stacking brackets later.
2. Position a bubble level on the
center top shelf of the
incubator and adjust the
leveling feet of the lower
incubator until the incubator is
level.
3. Lift the upper incubator onto
the top the lower incubator and
align them.
Note: Adequate care must be done
when lifting the incubator. It
usually takes 6 people to lift the
incubator if only manpower is available. The leveling feet are NOT to be removed. They can be adjusted to allow 28
mm to 45 mm (1.1” to 1.8”) clearance between the incubators.
4. There are 4 stacking brackets provided in the accessory kit box, 2 for the front and 2 for the back. Secure
the stacking brackets to the unit using the M4 screw provided (see illustration).
5. Tighten the nut on the foot to secure the stacking brackets.
2.2.3 ULPA Filter Installation (For 170L & 240L)

To install the ULPA filter, push it into the ULPA gasket and fit it to related mounting holes and mount the ULPA
filter to the top duct using thumb screws. Finally, connect the filter air exchange tubing to the ULPA filter (see
illustration below).
CO2 Incubator
10
2.2.3.1 Installing ULPA Filter from Old to Latest Configuration
Old ULPA filter configurations such as the collared and bracketed mounting types are now phased-out
due to issues encountered. Operators must check their units and change the ULPA filter mounting type
to the latest type (direct mounting) if their unit is still using any of the old types. Units without ULPA
filter (CCL-NF, 170 L and 240 L models only) can also be added with an ULPA filter with the latest
mounting type. Refer to the table below for the conversion kit needed when upgrading to the latest
filter design.
CO₂ Part number of

Design Type Incubator conversion kit needed Components of conversion kit
Volume (L) for the upgrade
1. Collared mounting ULPA filter (1250081), gasket
Type and No Filter 5071089 (1051440), stainless steel top
Type design (-NF) plenum
ULPA filter (1250081), 1 x SS
5072101
CCL-170_-_ adaptor bracket, 2 x gasket
(new simplified kit)
(1051440)
2018 NEW SIMPLIFIED KIT
5170989 (5072101) + 2x Inline filter
(1050771)
ULPA filter (1250081), gasket
5071144 (1051440), stainless steel top
plenum
ULPA filter (1250081), 1 x SS
5072101
CCL-240_-_ adaptor bracket, 2 x gasket
(new simplified kit)
(1051440)
2018 NEW SIMPLIFIED KIT
5170989 (5072101) + 2x Inline filter
(1050771)
2. Bracketed mounting Gasket: 1051440
No need for any kit
Filter: 1250081
CCL-170_-_ 1x Filter (1250081) + 1x Gasket
5170990 (1051440) + 2x Inline filter
(1050771)
CelCulture®
11
Gasket: 1051440
No need for any kit
Filter: 1250081
CCL-240_-_ 1x Filter (1250081) + 1x Gasket
5170990 (1051440) + 2x Inline filter
(1050771)
3. Direct Mounting Filter: 1250081
Note: The Gasket (1051440)
Type No need for any kit
is just required to order if it
CCL-170_-_ is damage.
1x Filter (1250081) + 2x Inline
5170992
filter (1050771)
Filter: 1250081
Note: The Gasket (1051440)
No need for any kit
is just required to order if it
CCL-240_-_ is damage.
1x Filter (1250081) + 2x Inline
5170992
filter (1050771)
To install the new simplified conversion kit
1. Prepare the simplified conversion kit.

2. Put gasket on the SS adaptor bracket matching the holes of both components.
3. Install the SS adaptor bracket with gasket to the old top plenum with the gasket facing the top
plenum. Ensure that the two small holes at the center area of the adaptor bracket matched
the holes on the top plenum.
4. Secure the SS adaptor bracket to the top plenum with 2 pcs. of screws on the 2 holes at the
center area.
5. Install the other gasket to the SS adaptor bracket, still matching the 4 holes at the edges of the
bracket and gasket.
6. Install the ULPA filter to the top plenum following the holes of the gasket (with the hollow side
facing the gasket).
7. Tighten the ULPA filter with 4pcs. of lock nut at the holes on the rim of the filter.
2.2.4 Shelves System Installation

Pilasters are held against the chamber walls using thumb screws.
1 There are 2 pilaster models – the shelf railing holders in the front
pilasters (2) is open upward, while the one in the back pilasters (1)
3 are open toward the back of the unit.
2
4 Shelf railings (3) are installed by sliding them in the shelf railing
holders in the back pilaster, then the front.
Slide in the shelves (4) in the openings of the shelf railings.
2.2.5 Connecting the Unit to Electrical Power

Plug the provided power cord to the power inlet at the rear of the unit. Connect the power cord to the building
outlet and turn ON the power switch of the outlet if necessary.
Note: Refer to the serial number tag located on the right side of the unit for electrical specification. Ensure the
unit is connected to the correct power source.
CO2 Incubator
12
2.2.6 Connecting the Unit to CO₂ and N2 Gas Supply

CO₂ gas supply cylinder and N₂ gas supply cylinder (for suppressed O2 model) should be industrial grade 99.5%
pure for CO₂ and 99.995% for N₂ and without siphon tube. Install a 2-stage pressure regulator to the tank outlet.
The inlet pressure must be regulated to 15 psig (103.4 kPa).
• Use the Connection Hose Kit provided in the

Accessories Kit Box. A 0.2 micron inlet filter (1) is
provided to remove any contaminant in the CO2
gas supply. Check all fittings for leaks.
• Connect one end of the tubing to the barb fitting
at the rear of the unit labeled CO₂ Inlet #1 and
install the clamp (2) to properly secure the tubing.
For Suppressed O2 model, connect another tube to
the N2 Inlet #1.
• Connect the other end of the tubing to the outlet
of the 2-stage pressure regulator and install the
clamp (2).
Note: Make sure that the pressure in the two stage gas
is regulated to 15 psig or 103.4 kPa.
If unit is equipped with the optional built-in gas backup system, there will be 2 gas inlets. Each of the inlet should
be connected to individual gas tanks as described above.
Note: Consult your facility safety officer to ensure that the equipment is installed in accordance to your local
regulations and code.
Caution: Avoid contact with CO₂ gas to eyes and skin. CO₂ and N₂ gases can have a suffocating effect in high
concentrations. During normal operations, small amounts of CO₂ and O₂ are released by the incubator to its
surroundings. Ensure that the room is well-ventilated to prevent suffocation of operators.
2.2.7 Connecting Alarm Contacts and RS485 Communication Port
A set of relay contacts located on the rear of the unit is provided to monitor for
temperature, humidity, O₂ or CO₂ alarms. The terminals provided on the alarm contact
allow for a NO (normally open) output, a NC (normally closed) and COM (common)
connection. In the event of an alarm condition, the NO contact will close, and the NC
contact will open. Once the alarm is cleared, the contacts return to their normal condition.
To activate this function, see section 3.3.12. Relay Activation.
The RS485 provides serial communication port for PC. It can be daisy chained from
product to product and connected to PC with Esco Voyager® Software.
2.3 Preliminary Cleaning

• Remove the tartan tape and the plastic protective coating on the shelf supports and air duct, if present.
• Use a suitable laboratory disinfectant to disinfect all interior surfaces prior to using the product. It is
recommended to use soft cloth with alcohol to remove adhesive residues.
Note: Please remove the adhesives (tape, covering, etc.) before performing the automated decontamination
cycle. Failure to do so will result to burnt marks or stains in the shelves and chamber.
Note: Metal surfaces can be cleaned using stainless steel cleaning agents. The inner glass door surfaces can be
cleaned using glass cleaners. Never use chlorine-based disinfectants. Do NOT spray disinfectant directly on the
control panel and on the holes for the electrical panel on the top-side of the unit.
CelCulture®
13
• See section 5.1 Cleaning Procedure for more information on cleaning the unit.
2.4 Filling the Humidity Pan

During normal operations, the humidity pan must be filled with the respective volume of water:
Size Normal (ml)

CCL-050L 400
CCL-170L 800
CCL-240L 800
For best operation and longevity of the incubator, sterile distilled water should only be used to fill the humidity
pan. Never fill the pan with more than the indicated maximum level.
Note: Chlorinated tap water should not be used as chlorine can deteriorate the stainless steel. Tap
water may also have a high mineral content, which would produce a build-up of scale in the humidity
pan. Water should always be sterilized or treated with a decontaminant, safe for use with stainless
steel as well as safe for the product, prior to being introduced into the humidity pan. If sensitive cells
are being cultured, do not add decontaminant in the water.
Place the pan directly on the incubator floor to ensure optimum humidity and temperature response. The pan
needs to be inserted completely into the back of the incubator so that condensed water can drip back into
the pan and avoid flooding the bottom of the chamber. Ensure that no water is spilled on the bottom of the
chamber as this will potentially lead to condensation.
If water pan is filled with more than the indicated maximum level and / or not pushed to the back surface of the
chamber, condensation will occur.
It is recommended to check the water level and refill the humidity pan once a week.
For 170/240L Suppressed O2 model, ensure that the sparger is fully immersed in water.
When the incubator is turned off and will not be used for a period of time, empty the water pan and
dry it before putting it back inside the unit. Leaving the humidity pan with water when unit is
powered off will cause condensation to form in the chamber which will damage the sensors.
2.5 Filtered Air Exchange (For 170L & 240L)

Filtered air exchange is an intentional ‘leak’ in the chamber to reduce the relative humidity to an acceptable
level (85-90%) and to ensure no condensation occurs within the chamber. A small amount of ambient air is being
drawn from outside of the incubator by the re-circulating fan, through a tubing and 1 μm filter to prevent
contamination in the chamber. Air is being ‘pushed out’ through another tubing and 1 μm filter installed on the
access port plug located at the back of incubator. Tubing installed inside the chamber for filtered air exchange
is silicon tubing which can withstand high decontamination temperature.
If high humidity level is required, the filtered air exchange can be removed and “Elevated Humidity” can be
activated in the control panel (call Esco or your distributor to activate this function).
When set to elevated humidity mode, the incubator may be able to achieve up to 85%-90% relative humidity
depending on the humidity level that the unit can achieve in default humidity mode (usually +2-3% of value from
default humidity).
CO2 Incubator
14
2.6 Use of Additional Devices in the CO₂ Incubator

Placing heat-generating electrical devices in the chamber might affect the incubator’s performance. The unit
must be recalibrated or revalidated by a trained service personnel based on this application.
Use only the sampling port or the rear access port(s) for tubing or cable access. Never route any cable or tubing
through the door opening as it will disrupt the environment of the chamber.
2.7 First Run

After the incubator has been properly installed, connected to the power supply, humidity pan filled, and the unit
connected to the gas supply, set the pressure inlet to the incubator at the range of 15-17 psi. Recommended
pressure is 15.5 -16 psi depending on the length of the tube from the gas tank to the incubator.
Follow the procedures for the unit’s initial start-up.
1. Switch ON the unit. A welcome message will appear on the display. Press SET to continue.
2. Keypad operation will appear on the display. Press SET to continue.
3. Set the date, time, temperature set point, %CO₂ set point and %O 2 set point:
a. Set Date. The digit will blink. Use UP/DOWN buttons to select the current date and press SET
to confirm each digit.
b. Set Time. The digit will blink. Use UP/DOWN buttons to select the current time and press SET
to confirm each digit.
Note: To save power, the LCD backlight will automatically turn off from 11:00 pm to 6:00 am daily.
c. Set Temperature Set Point. Use UP/DOWN buttons to select the temperature set point. Press
SET to confirm.
d. Set %CO₂ Set Point. Use UP/DOWN buttons to select the %CO₂ set point. Press SET to confirm.
e. Set %O2 Set Point. Use UP/DOWN buttons to select the %O₂ set point. Press SET to confirm.
(For suppressed O2 model only)
f. Setting the tank reminder alarm (single tank for tank replacement prediction). Set NEW CO₂
TANK. Set the CO₂ gas net weight (refer to the gas catalog from the supplier). The unit is set to
0031Kg by factory default for a single gas tank for single incubator consumption. If 1 gas tank
supplies several incubators, divide gas net weight with the number of the supplied incubators.
Tank reminder alarm will activate to alert the user before gas supply is depleted completely.
4. Incubator set-up is done.
5. The unit will run a memory check.
6. The incubator will do an initial start-up and will continue to the main menu.
The outer door has a magnetic closure, which can be opened by pulling on the rim of the door. The inner glass
door has a tight gasket seal, which prevents the chamber from being contaminated and the chamber
environment from being disturbed.
When the outer door is opened the blower fan, heating, and gas injection will automatically turn off.
Note: Make sure that the door switch will not be overridden or controlled manually or by any means when the
outer door is opened. During normal operations, never leave the door open as it will cause the incubator to
overheat and may pose a fire hazard.
Allow 2 hours for the incubator to stabilize before loading the unit with samples or before proceeding to
calibration.
Note: When loading the unit, ensure proper spacing between the samples to allow uniform air circulation inside
the chamber. Never place any item touching the side walls of the incubator as it may take a longer time to reach
the set temperature. For your safety, never load samples with unknown or potentially harmful composition.
CelCulture®
15
2.8 UV Lamp Functionality (For CCL-UV units)

The UV lamp is located at the bottom rear part of the chamber. The main function of the UV lamp is to sterilize
the water in the humidity pan and the air circulating in the chamber.
Always use the humidity pan and humidity pan cover to prevent UV light from escaping.
To check whether the UV lamp is lit, activate the test mode by pressing up, up and set button simultaneously.
Activate it by switching the UV to ON mode. Open the outer door and then press the door switch with the inner
glass door closed. The appearance of visible blue light from the front of the humidity pan cover confirms that
the UV lamp is lit.
Caution: Radiation Hazard
UV functionality check must be conducted within less than 10 seconds to ensure zero
risk of UV radiation. UV light is harmful to the eyes, so do not light the UV lamp when
the inner door or humidifying pan cover is open.
2.9 Replacing Old CO₂ Sensor to the Latest IR Sensor

The TC sensor (which is less reliable) and the previous IR CO₂ sensor (which uses board, cable, and probe) of Esco
CelCulture® CO₂ incubators are now phased out. Upgrading to the new IR CO₂ sensor (which is a stand-alone
sensor) is recommended to improve the reliability and ease of handling of the sensor. Contact your local
distributor for the upgrade kit to the new IR sensor (item number 1250105).
Note: Replacement of the old sensor with the new one must only be undertaken by qualified service personnel
to prevent damage to the equipment.
2.10 Disclaimer
Each of the Esco units is subjected to an in-depth inspection prior to delivery. Signs of use caused by tests are
possible and have no influence on the performance and function of the units. The performance and safety of
the incubator, while rigorously evaluated at the factory, cannot be guaranteed after transit and installation.
Therefore, on-site testing is always recommended.
CO2 Incubator
16
Chapter 3 - Control System and Operation

3.1 CelCulture® Control System
DECON LED HEAT LED oC/%RH Display MENU Button Main LCD UP Button ALARM LED
DECON Button INJECT LED %CO2/%O2 Display SET Button DOWN Button MUTE Button
DECON Button & DECON LED
• Activates 90oC moist heat decontamination procedure

• During decontamination procedure, the yellow DECON LED will light up.
MENU Button
• When the main LCD shows normal display, pressing the MENU button will display the menu options.
• Within the menu display, pressing the MENU button will bring back the previous menu level.
SET Button
• Within the menu display, pressing the SET button will confirm a selection or value.
UP/DOWN Buttons
• Within the menu display, pressing the UP and DOWN buttons will scroll the display up and down.
• When an input is required, pressing the UP and DOWN buttons will increase and decrease a value.
MUTE Button & ALARM LED
• MUTE button will mute the audible alarm for a period of time.
• The red ALARM LED will light up whenever an alarm condition is triggered.
HEAT LED
• HEAT LED will light up whenever a heating process is activated.
INJECT LED
• INJECT LED will light up whenever a gas injection process is activated.
o
C/%RH Display
• When oC is displayed, the display indicates the temperature in the chamber.
CelCulture®
17
• When %RH is displayed, the display indicates the relative humidity in the chamber (optional).
%CO₂/%O2 Display
• When %CO₂ is displayed, the display indicates CO₂ concentration level in the chamber
• When %O2 is displayed, the display indicates O2 concentration level in the chamber (for suppressed O 2
model only)
CO2 Incubator
18
3.2 Menu Options

SET TEMP
SETTINGS SET %CO2

TEMPERATURE SENSOR
SET %O2
%CO2 SENSOR
CALIBRATION
OVER TEMP ALARM %O2 SENSOR
LOW TEMP ALARM %RH SENSOR
HIGH %CO2 ALARM
LOW %CO2 ALARM
NEW CO2 TANK
HIGH %O2 ALARM
LOW %O2 ALARM
LOW %RH ALARM
NEW PIN
AUDIBLE ALARM
ALARM RING BACK
DOOR ALARM DELAY
ADMIN TEMP ALARM RELAY
%CO2 ALARM RELAY
%RH ALARM RELAY
%O2 ALARM RELAY
SET ULPA REMINDER
REPLACE ULPA FILTER

MENU
SET LANGUAGE
SET DATE
SET TIME
SET RS-485 ADDRESS SHOW TEMP DATA LOG
RESET DEFAULTS SHOW %CO2 DATA LOG
DATALOGGING SHOW %O2 DATA LOG

DIAGNOSTIC READ OUT
SHOW %RH DATA LOG
SHOW WARNING LOG
DATA LOG PERIOD
SHOW TIME AND DATE
SERVICE CALIBRATE A/O TEMP
CALIBRATE A/O %CO2 USE THE KEYS
CALIBRATE A/O %O2 USE DECON CYCLE
CALIBRATE A/O %RH TERMINATE DECON
HELP CONFIGURE ALARM
SET PIN
SET TEMP/%CO2
Note: Menu in the darker boxes are shown in special circumstances.
MORE HELP
CelCulture®
19
3.2.1 Settings
3.2.1.1 Set Temperature

Your incubator has an operating temperature range of ambient +7°C to 60°C. The default temperature set point
is 37oC. Range control is 20.0 < T (°C) < 60.0. Temperature control will be disabled when set at 20.0°C and at
60.0°C. Set point for temperature can be adjusted in steps of 0.1 °C. Set the temperature to 59.9°C if 60°C is
needed for incubation, and set to 20.1°C if 20°C is needed.
MENU SETTINGS SET TEMP 20 < X < 60
3.2.1.2 Set %CO₂

Your Incubator has a range of 0% -19.5% CO₂. The default CO₂ set point is 5%. Range control is 0 < %CO₂ < 19.5.
Control for %CO₂ will be disabled when set at 0.0% and at 19.5%. Set point for %CO₂ can be adjusted in steps of
0.1%. Set the %CO₂ to 19.4% if 19.5% is needed for incubation.
MENU SETTINGS SET %CO2 0 0<<XX<< 19.5

20
3.2.1.3 Set %O2 (for suppressed O2 model)

Your Incubator has a range of 1% -18% O₂. The default O₂ set point is 5%. Range control is 1.0 ≤ %O₂ < 18. Control
for %O₂ will be disabled when set at 18%. Set point %O₂ can be adjusted in steps of 0.1%.
MENU SETTINGS SET %O2 1.01 ≤< X%O₂

< 20.7
< 18
3.2.2 Calibration
3.2.2.1 Unit Calibration

After the unit has stabilized (recommended to run overnight), the temperature and CO₂ sensors can be
calibrated. In the calibration mode, the temperature, CO₂, O2 (for suppressed O2 model), and RH (for units with
optional humidity display) reading can all be calibrated to reference instruments. To determine the exact
measured value of the sensors, a comparison measurement has to be performed every year. If a major deviation
is found during this check, calibration of the sensor is required. This is to ensure continuous and optimal
performance of the CO₂ incubator.
A calibrated measuring instrument with higher accuracy as a reference instrument is required.
Place the reference instrument or its measuring probe on the center of work zone. Route the connecting cable
through the access port located at the rear of work zone, and highly to seal the access port tightly once cables
are placed there. Close doors and wait until the value displayed in the reference instrument is stabilized. Record
the measured value.
Enter MENU|CALIBRATION and select the responding options. When asked, enter the measured value. Press
SET to confirm.
When calibrating the zirconia O₂ sensor: If the difference value between the incubator’s display reading and
the reading from the reference instrument is ≥1.0%, it is recommended to do resetting of the O₂ sensor following
the instructions below.
CO2 Incubator
20
Resetting the O₂ sensor: When resetting the O₂ sensor, prepare an electronic gas analyzer and the magnetic
rear access port plug then follow below procedure:
By default magnetic rear access port plug for By default magnetic rear access port plug for 50L
170/240L is installed directly to the unit from the is NOT installed directly to the unit from factory.
factory. This plug can be found in retrofit kit box.
For 50 L models:
1. Disconnect CO2 and N2 gas injection from the unit. No gas allowed during doing reset calibration.
2. Open the outer door and the inner glass door to allow the temperature in the chamber to be
similar to the ambient temperature (e.g. ambient temperature is 25°C, then recommended
incubator temperature on display should be around 24°C to 26°C).
3. Once temperature has stabilized, remove the original plug and install the rear access port plug
with magnet to the left rear access port hole inside the chamber for 10 seconds then check the %O₂
actual display on the incubator control panel which should have changed to 17.7% to 18%.
CelCulture®
21
4. Remove the rear access port plug with magnet and install back the original plug without magnet to
the left rear access port hole and close the chamber doors. Set the %CO₂ and %O₂ to 5.0% and
allow gas to be injected.
5. Allow the unit to stabilize for 1 hour, then measure the %CO₂/%O₂ concentration by inserting
around 10cm of electronic gas analyzer tubing through the sampling port on the glass door of the
chamber. Turn on the pump of the gas analyzer and wait for 5 minutes when the O₂ reading has
stabilized.
6. Check if there is a difference between the readings from the gas analyzer and from the incubator
actual display. Set the O₂ offset (MENU > CALIBRATION > %O₂ SENSOR > CALIBRATE) when needed.
For 170/240 L models:
1. Disconnect CO2 and N2 gas injection from the unit. No gas allowed during doing reset calibration.
2. Open the outer door and the inner glass door to allow the temperature in the chamber to be similar to
the ambient temperature (e.g. ambient temperature is 25°C, then recommended incubator
temperature on display should be around 24°C to 26°C).
3. Once temperature has stabilized, install the rear access port plug with magnet from left side to the
right rear access port hole inside the chamber for 10 seconds. Check the %O₂ actual display on the
incubator control panel which should have changed to around 17.7 % - 18%.
Transfer the plug from the left

access port hole to the right hole.
Plug inside the chamber.
Rear access port plug with magnet
4. Remove the rear access port plug with magnet and install back to the left rear access port hole and
close the chamber doors. Set the %CO₂ and %O₂ to 5.0% and allow gas to be injected.
CO2 Incubator
22
5. Allow the unit to stabilize for 1 hour, then measure the %CO₂/%O₂ concentration by inserting around
10cm of electronic gas analyzer tubing through the sampling port on the glass door of the chamber.
Turn on the pump of the gas analyzer and wait for 5 minutes when the O₂ reading has stabilized.
6. Check if there is a difference between the readings from the gas analyzer and from the incubator actual
display. Set the O₂ offset (MENU > CALIBRATION > %O₂ SENSOR > CALIBRATE) when needed.
To reset the temperature, CO2, and RH sensors to its default calibration, select DEFAULT CALIBRATION. Press
SET button to confirm.
3.2.2.2 Analog Output Calibration (for units with optional analog output)
A set of relay contacts are provided at the rear of the incubator to allow the unit to output analog signals
representing the temperature, %CO₂, %O2 (for suppressed O2 model), and %RH (for units with optional humidity
display) depending on the options available in your incubator. This allows the chamber to be connected to an
in-house data acquisition or alarm system.
The analog data output can be set to operate in either DC (0-5V) or current (4-20mA) mode. The factory default
setting is voltage. The voltage of the analog output can be calibrated using a calibrated digital multimeter.
To calibrate Rear Panel Terminal MENU | SERVICE

Temperature Analog Output Temp (+ and -) Calibrate A/O Temp
CO₂ Analog Output CO₂ (+ and -) Calibrate A/O CO₂
% Relative Humidity Analog Output RH (+ and -) Calibrate A/O RH
O2 Analog Output O2 (+ and -) Calibrate A/O O₂
Place the multimeter’s measuring probe on the responding + and - terminals of the analog output located on
the rear panel and record the measured value.
Enter MENU|SERVICE and select the responding options. When asked, enter the measured value. Press SET to
confirm.
3.2.3 Admin
3.2.3.1 Over Temperature Alarm

The unit will display/sound the alarm when the temperature goes above the over temperature alarm value. The
default over temperature alarm is temperature set point + 1oC.
MENU ADMIN OVER TEMP ALARM SP + 1 ≤ X ≤ 65
3.2.3.2 Low Temperature Alarm

The unit will display/sound the alarm when the temperature goes below the low-temperature alarm value. The
minimum low temperature alarm value that can be set in all CCL models except CCL with Peltier Cooling System
is 19.0°C. The minimum low temperature alarm value in CCL with Peltier Cooling System is 0.0°C. The default
low-temperature alarm is temperature set point - 1oC.
Note: The low-temperature alarm will only be activated once the unit reaches the temperature set point.
MENU ADMIN LOW TEMP ALARM 0 ≤ X ≤ SP - 1
3.2.3.3 High %CO₂ Alarm

The unit will display/sound the alarm when the CO₂ level goes above the high %CO₂ alarm value. The default
high %CO₂ alarm is %CO₂ set point + 1%.
MENU ADMIN HIGH %CO2 ALARM SP + 1 ≤ X ≤ 21
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23
3.2.3.4 Low %CO₂ Alarm

The unit will display/sound the alarm when the CO₂ level goes below the low %CO₂ alarm value. The default low
%CO₂ alarm is %CO₂ set point - 1%.
MENU ADMIN LOW %CO2 ALARM 0 ≤ X ≤ SP - 1
3.2.3.5 New CO₂ Tank (for unit without the optional gas backup switch system)
The CO₂ tank depletion reminder automatically calculates how much CO₂ gas is left in the tank. It alerts the user
one week before the gas is depleted which gives the user some buffer time to order new tanks.
MENU ADMIN NEW CO2 TANK XXXX
3.2.3.6 High %O2 Alarm (for suppressed O2 model)

The unit will display/sound the alarm when the O 2 level goes above the high %O2 alarm value. The default high
%O2 alarm is %O2 set point +1%.
MENU ADMIN HIGH %O2 ALARM SP + 1 ≤ X ≤ 21
3.2.3.7 Low %O2 Alarm (for suppressed O2 model)

The unit will display/sound the alarm when the O2 level goes below the low %O2 alarm value. The default low
%O2 alarm is %O2 set point -1%.
MENU ADMIN LOW %O2 ALARM 0 ≤ X ≤ SP - 1
3.2.3.8 Low %RH Alarm (for unit with optional RH display)

The unit will display/sound the alarm when the RH level goes below the low %RH alarm value. The default low
%RH alarm is 75%.
MENU ADMIN LOW %RH ALARM 00 <
≤ XX≤<9075
3.2.3.9 New PIN

PIN restricts access to Menu functions. User must enter 4-digit PIN before accessing MENU. Default PIN is 0000
(Disabled).
MENU ADMIN NEW PIN XXXX
3.2.3.10 Audible Alarm

Audible alarms can be enabled or disabled, however the visual alarm will still be active even after the audible
alarm is disabled.
MENU ADMIN AUDIBLE ALARM ENABLE | DISABLE
3.2.3.11 Alarm Ring Back Time

When the system encounters an error, it will sound the audible alarm. The user can temporarily press MUTE
button to disable the sound. If the error is not corrected within the time set within this option, the audible alarm
will be re-enabled.
MENU ADMIN ALARM RING BACK 0 ≤ X ≤ 30
3.2.3.12 Door Alarm Delay Time

To set the delay time for door alarm. Delay time can be set between 1 to 15 minutes. Default is 15 minutes.
MENU ADMIN DOOR ALARM DELAY 1 ≤ X ≤ 15
3.2.3.13 Relay Activation

SPDT switch can be wired as normally open (NO) or normally close (NC) and common (COM). Rating of the switch
is maximum 30VDC, 2A. The purpose of alarm relay is to facilitate monitoring. When there is a fault in the system
pertaining to the relevant parameter (temperature, %CO₂, %O₂ (for suppressed O 2 model) or %RH (for units with
CO2 Incubator
24
optional %RH display), the relay will make or break to signal to a remote device that the incubator is in a faulty
condition.
TEMP ALARM RELAY ENABLE | DISABLE
%CO2 ALARM RELAY ENABLE | DISABLE

MENU ADMIN
%O2 ALARM RELAY ENABLE | DISABLE
%RH ALARM RELAY ENABLE | DISABLE
3.2.3.14 Set ULPA Reminder (For 170L & 240L models)

The ULPA Reminder will alert the user to replace ULPA filter at a set period. Default is set to 12 months.
MENU ADMIN SET ULPA REMINDER 0 ≤ X ≤ 12
3.2.3.15 Replace ULPA Filter (For 170L & 240L models)

The replace ULPA filter settings must be set when replacing ULPA filter in order for the set ULPA Reminder to
reset based on the set period.
MENU ADMIN REPLACE ULPA FILTER
3.2.3.16 Set UV Schedule (For units with UV lamp only)

The UV lamp will turn on based on the set UV schedule. Default is 02:00 AM.
MENU ADMIN UV SCHEDULE HHMM
3.2.3.17 Set UV Timer (For units with UV lamp only)

The UV Timer determines how long the UV lamp will light up based on the preset period of time. Default is 30
minutes.
Note: If the outer door is opened while the UV lamp is lit, the lamp will turn off. When the door is closed, the lamp will
light for the preset period of time.
MENU ADMIN UV TIMER 00 ≤ MM ≤ 60
3.2.3.18 Set UV Reminder (For units with UV lamp only)

The UV Reminder will alert the user to replace the UV lamp. The recommended replacement time for the UV
lamp is when the accumulated active time reaches 1000 hours (default).
MENU ADMIN SET UV REMINDER 0000 ≤ HHHH ≤ 9999
3.2.3.19 Replace UV Lamp (For units with UV lamp only)

The Replace UV Lamp setting must be set when replacing UV lamp in order for the Set UV Reminder to reset
based on the set period.
MENU ADMIN REPLACE UV LAMP
3.2.3.20 Set Language

List of language available includes English, Spanish, German, French and Italian.
ENGLISH
SPANISH
MENU ADMIN SET LANGUAGE GERMAN
FRENCH
ITALIAN
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25
3.2.3.21 Set Date

User can set the date and it will be maintained even after the unit is turned off. The format is YYYY MM DD.
MENU ADMIN SET DATE YYYY MM DD
3.2.3.22 Set Time

User can set the time and it will be maintained even after the unit is turned off. The format is HHMMSS.
MENU ADMIN SET TIME HHMMSS
3.2.3.23 Set RS-485 Address (for unit with optional Voyager® monitoring software)
User can set the RS-485 address for PC connection.
MENU ADMIN SET RS-485 ADDRESS 1 ≤ X ≤ 32
3.2.3.24 Reset Default

User can reset the unit to the factory default settings by choosing this option. The features being reset are all
set points and alarms.
MENU ADMIN RESET DEFAULT
3.2.4 Data Logging
3.2.4.1 Show Data Log

User can set to show temperature, %CO₂, %O2 (for suppressed O2 model) and %RH (for units with optional RH
display) data log. The data format is MMDDYY HHMM INFO. Use the UP/DOWN buttons to read through the log.
Press MENU to go back to previous level.
SHOW TEMP DATA LOG
SHOW %CO2 DATA LOG

MENU DATALOGGING
SHOW %O2 DATA LOG
SHOW %RH DATA LOG
3.2.4.2 Data Log Period

The Data Log Period allows the user to specify the period in between the system log the data. The period can be
set from 0 minute up to 24 hours.
MENU DATALOGGING DATA LOG PERIOD 10MINUTES
MINS ≤ ≤X X≤ ≤24
24HOURS
HOURS
3.2.5 Service
3.2.5.1 Diagnostic Readout

This menu will show all parameters and set points that are currently being set or operating in the incubator. See
section 3.4 Diagnostic Readout. Use the UP/DOWN buttons to read through the readout. Press MENU to go
back to the previous level.
MENU SERVICE DIAGNOSTIC READOUT
3.2.5.2 Show Warning Message

This will display the description of the error with date and time. The user can scroll down or up if the error
message is too long. It will only display up to a maximum of 32 error messages after which the new errors will
override the old error messages. Use the UP/DOWN buttons to read through the log. Press MENU to go back to
the previous level.
MENU SERVICE SHOW WARNING LOG
CO2 Incubator
26
3.2.5.3 Show Date & Time

Shows the actual time and date – based on the value input in set time and set date or during the first run.
MENU SERVICE SHOW TIME & DATE
3.2.5.4 Calibrate Analog Output

See section 3.3.2 Analog Output Calibration for the instruction.
3.2.6 Help
This menu section will show brief explanation on the basic control of the unit.
3.3 Operation of CO₂ Incubator with Peltier Cooling System (applicable for CCL-P
series)
3.3.1 Cooling Mode

To activate the cooling mode, set the temperature to any point lower than the currently displayed value by
accessing settings. Press SET button once, press MENU to go back to normal display and wait for 4-5 seconds
until the operation mode display appears as shown below:
Cooling mode control range: 8oC below ambient
Note: The incubator is working according to the room temperature. Stable room/ambient temperature is
therefore required. When the temperature set point is equal to or below the ambient temperature, put empty
humidity pan in the chamber or if it has water, drain first and put back the dried pan inside the chamber.
3.3.2 Heating Mode

To activate the heating mode, set the temperature to any point higher than the currently displayed value by
accessing settings. Press SET button once, press MENU to go back to the normal display and wait for 4-5 seconds
until the operation mode display appears as shown below:
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3.4 Diagnostic Readout

Messages Description
MAIN_BOARD Main board firmware version.
DISP_BOARD Display board firmware version.
TEMP READ Snap shot of actual temperature reading.
ADC TEMP Snap shot of actual Analog to Digital Conversion value of temperature read.
TEMP OFFSET Offset value applied for temperature reading on 7 segment display.
TEMP SP Temperature set point.
OV TEMP SP Over temperature alarm set point.
LO TEMP Low temperature alarm set point.
%CO₂ READ Snap shot of actual CO₂ level read.
CO₂ OFFSET Offset value applied for CO₂ level reading on 7 segment display.
%CO₂ SP CO₂ level set point.
HI %CO₂ SP High CO₂ level alarm set point.
LO %CO₂ SP Low CO₂ level alarm set point.
%O2 READ Snap shot of actual O2 level read.*
ADC %O2 Snap shot of actual Analog to Digital Conversion value of O2level read.*
%O2 SLOPE Offset value applied for O2level reading on 7 segment display.*
%O2 SP O2level set point.*
HI %O2 SP High O2 level alarm set point.*
LO %O2 SP Low O2 level alarm set point.*
%RH READ Snap shot of actual RH level read.**
ADC %RH Snap shot of actual Analog to Digital Conversion value of RH level read.**
%RH OFFSET Offset value applied for RH level reading on 7 segment display.**
%RH SETTING Humidity level setting. High humidity will show LVTD. Default humidity will show DFLT.
LO %RH SP Low RH level alarm set point.**
DOOR SWITCH Door switch status of OPEN or CLOSE.
DOOR DELAY Door open alarm delay set point.
THERMOSTAT Thermostat status of OPEN or CLOSE.
MAIN HTRDCY Percentage of average duty cycle of the main heater work over an hour.
BASE HTRDCY Percentage of average duty cycle of the base heater work over an hour.
DOOR HTRDCY Percentage of average duty cycle of the door heater work over an hour.
%CO₂ INJDCY Percentage of average duty cycle of the CO₂ solenoid valve work over an hour.
%O2 INJDCY Percentage of average duty cycle of the O2solenoid valve work over an hour.*
If CO₂ BACKUP is not installed then it will show the percentage of remaining capacity of CO₂ tank.
CO₂ TANK 1
If CO₂ BACKUP is installed then it will show OK or NOK of the CO₂ tank capacity.***
CO₂ TANK 2 Will show OK or NOK of the CO₂ tank 2 capacity.***
N2 TANK 1 Will show OK or NOK of the N2 tank 1 capacity.****
N2 TANK 2 Will show OK or NOK of the N2 tank 2 capacity.****
AUDIBL ALRM Audible alarm status of “EN”able or “DIS”able.
RING BACK T Current ring back time value
DA DELAY Door Alarm Delay period: 5 mins.
TEMP RELAY Temperature alarm condition is “EN”able or “DIS”able for activating the remote alarm relay.
CO₂ RELAY CO₂l evel alarm condition is “EN”able or “DIS”able for activating the remote alarm relay.
O2 RELAY O2 level alarm condition is “EN”able or “DIS”able for activating the remote alarm relay.*
RH RELAY RH level alarm condition is “EN”able or “DIS”able for activating the remote alarm relay.**
ULPA REMAIN ULPA remaining life time in months.
ADDRESS RS-485 address for this CO₂ Incubator.
LOG PERIOD Log period applied in minutes or hours.
FLASH MEM Total of FLASH memory used to keep the record of data log or warning log.
* Only available for Suppressed O2 model
** Only available for units with RH display options installed
*** Only available for units with CO₂ backup options installed
**** Only available for units with N2 backup options installed
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28
3.5 Alarm
The CelCulture® CO₂ Incubator alarm system is shown in the table below. When an alarm is active, the error message appears
in the display of the controller. Pressing MUTE button will disable the audible alarm for the ring back period. However, the
visual alarm continues until the incubator returns to a normal condition.
Alarm name Condition indicated Alarm Delay Additional notes

ADC reading is in overflow or reads less than 10
Temp sensor error No delay 7 seg display will show "---"; heating will be disabled
counts
CO₂ sensor error No delay 7 seg display will show "---"; CO₂ injection will be disabled
counts
O2 sensor error* No delay 7 seg display will show "---"; N2 injection will be disabled
counts
RH sensor error** No delay 7 seg display will show "---"
counts
Temp reading plus calibration offset is greater
Over temperature No delay Heating is disabled, display shows temp reading
than overtemp setting
CO₂ reading plus calibration offset is greater
High CO₂ level 15 minutes CO₂ injection is disabled, display shows CO₂ reading
than high CO₂ setting
O2 reading plus calibration offset is greater than
High O2 level* 15 minutes N2 injection will not stop. Display shows O2 reading
high O2 setting
Door has been open for over ‘DOOR ALARM All gas injections and chamber fan stop without delay.
Door open 1 - 15 minutes
DELAY’ time Heating disabled.
%RH reading plus calibration offset is lower than
Low %RH** 2 hours Display continues to report %RH as read
low %RH setting
Temp reading plus calibration offset is lower
Low temperature 15 minutes Display continues to report temp as read
than low temp setting
CO₂ reading plus calibration offset is lower than
Low CO₂ level 15 minutes Display continues to show CO₂ reading
low CO₂ setting
O2 reading plus calibration offset is lower than Display continues to show O2 reading, N2 injection should
Low O2 level* 15 minutes
low O2 setting be disabled
Pressure switch on both CO₂ tanks indicate low
All CO₂ tanks low No delay Display continues to show CO₂ reading
pressure
All N2 tanks Pressure switch on both N2 tanks indicate low
No delay Display continues to show O2 reading
low**** pressure
Pressure switch on CO₂ tank 1 indicate low Alert the user to change the CO₂ tank (approx. 1 week
CO₂ tank 1 low 15 minutes
pressure and no backup before tank empty). Display shows CO₂ reading.
Pressure switch on CO₂ tank 1 indicate low Solenoid control automatically switches to CO₂ tank 2.
CO₂ tank 1 low*** 15 minutes
pressure and backup is installed Display shows CO₂ reading
Pressure switch on CO₂ tank 2 indicate low Solenoid control automatically switches to CO₂ tank 1.
CO₂ tank 2 low*** 15 minutes
pressure and backup is installed Display shows CO₂ reading
Pressure switch on N2 tank 1 indicate low Solenoid control automatically switches to N2 tank 2.
N2 tank 1 low**** 15 minutes
pressure and backup is installed Display shows O2 reading
Pressure switch on N2 tank 2 indicate low Solenoid control automatically switches to N2 tank 1.
N2 tank 2 low**** 15 minutes
pressure and backup is installed Display shows O2 reading
Timer reminder for ULPA replacement has been
ULPA reminder No delay
reached
During normal operations, rear access port must be
CLOSE REAR Reminder to close or plug back rear access port
No delay closed. Opening the rear access port plug can reduce the
ACCESS***** plug after decontamination complete
humidity and will cause the gas to leak
OPEN REAR Reminder to open or unplug rear access port During Decon cycle, rear access port must be open as
No delay
ACCESS***** plug before decontamination started moist heat exhaust port
Overshooting of temperature display to
BACKUP + 0.4°C of the setpoint for more than 30
RUNNING
No delay The alarm can be cleared after a door opening.
times indicates failure in the mainboard
setting system.
The system will automatically stop the solenoid
%CO2 display stuck at <0.5% over the
valve from injecting CO2 to prevent depletion of
CHECK CO2 setpoint indicates failure in CO2 sensor
SYSTEM
No delay CO2 tank supply. Users will be able to clear the
probe, CO2 solenoid valve, and/or the CO2
message and return the CO2 injection system by
tank supply.
opening and closing the door.
* Only available for Suppressed O2 model
** Only available for units with RH display options installed
*** Only available for units with CO₂ backup options installed
**** Only available for units with N2 backup options installed
***** Only available for 170L and 240L units
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29
Chapter 4 - General Maintenance

4.1 Scheduled Maintenance
Proper and timely maintenance is crucial for trouble-free functioning of any device, and your CO₂ incubator is
no exception to this rule. We strongly recommend that you follow the maintenance schedule suggested
hereunder in order to obtain optimal performance from your incubator.
Maintenance to be carried out
No Description of Task to Perform
Daily Weekly Yearly As Needed
1 Check CO₂/N₂ gas tank level √
2 Check water level in the humidity pan √
3 Cleaning the interior and exterior of incubator √
4 Calibration of O2 sensor √ √
5 General Inspection √
6 Calibration of temperature sensor √
7 Calibration of CO₂ sensor √
8 Calibration of humidity sensor √
9 Change ULPA filter (170L/240L) √
10 Change inlet filters √
11 Change outer door magnetic gasket √ √
12 Decontamination √
13 Change UV lamp (for units with UV lamp) √
Cleaning instruction for Cu model: Copper (Cu) chamber interior, after a period of time, will have discoloration.
Use vinegar, baking soda or lemon soap to clean the chamber surface. About 1 tablespoon of salt per 1 cup of
vinegar can be used to remove tarnishes. If there is a patina (greenish stain), use smooth/fine sandpaper to
remove the stain.
4.1.1 Check CO₂/N2 Gas Tank Level

Check the pressure gauge on the two-stage gas regulator daily to ensure the pressure is not below 15 psig.
Replace with a new tank if necessary.
4.1.2 Check Water Level in the Humidity Pan

Check weekly to ensure there is sufficient water in the pan. It is recommended to check the water level and refill
the humidity pan with sterile distilled water once a week.
4.1.3 Cleaning and Decontamination

See Chapter 5–Cleaning & Decontamination
4.1.4 General Inspection

Check the following regularly – at least annually:
• Tightness of the glass door seal
• Functional check of the operating panel and device control
• Electrical safety check in accordance with the relevant regulations
• Check tightness of hinge screws on door’s moving parts
• Check the latch of glass door if working properly and able to close tight
4.1.5 Calibration of Temperature, CO₂, O2 and Humidity

See section 3.2.2 - Calibration
4.1.6 Change ULPA Filter (CCL-170L & CCL-240L only)

See section 2.2.3 - ULPA Filter Installation
CO2 Incubator
30
4.1.7 Change Inlet Filter

Inlet filters are installed to remove any contaminant in the CO₂ or N2 gas supply. It prevents accumulation of dirt
in the gas inlet solenoid valves and tubes leading into the work area of the incubator. Change the inlet filters at
least once a year or when the filter is dirty by visual inspection. Use the hose clamp to secure the tubing to the
filter to ensure a gas-tight connection.
4.1.8 Change Outer Door Magnetic Gasket

The magnetic door gasket installed in the outer door of the incubator helps to keep a tight seal around the
chamber opening. It is recommended to check the magnetic door gasket for signs of brittleness, corrosion,
wearing, or any form of damage once a year. Replace the outer door magnetic gasket if it has been damaged or
when necessary.
4.1.9 Change UV Lamp (for units with UV lamp)

The UV lamp ensures that the circulating air and the water reservoir remains free of microorganisms and
contaminants. It is isolated from the inner chamber by the lower and back plenum cover to ensure that the
cultures are not affected by UV decontamination. To change the UV lamp, remove the back and lower plenum
covers by unscrewing them from the chamber. Replace the UV lamp and reinstall the plenum covers. Make sure
that the plenum covers are reinstalled properly and that the UV lamp is fully covered to avoid UV leak into the
culture chamber. It is advisable to change the UV lamp every 1000 hours of running time (approximately around
4 years).
4.2 Maintenance/Service Log

It is good practice (and in some cases regulatory requirement) to maintain a log of all maintenance work carried
out on your incubator.
NO. DATE/TIME DESCRIPTION OF MAINTENANCE/SERVICE TAKEN DONE BY REMARKS

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
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Chapter 5 - Cleaning & Decontamination

5.1 Cleaning Procedure
1. During the cleaning process, the operator should use Personal Protective Equipment (PPE) according to
the provisions of the laboratory.
2. Prepare the materials needed for the cleaning process such as mild soap solution, distilled water in a
wash bottle, sponge, clean cloth or tissue, disinfectant, and wash tray or bucket (if a sink is not
available).
Note: Metal surfaces can be cleaned using stainless steel cleaning agents. The inner glass door surfaces
can be cleaned using glass cleaners. Never use chlorine-based disinfectants!
3. Transfer all samples to another CO₂ incubator or store them in a safe place.
4. Turn off and unplug the device. If needed, mark that the unit is deactivated or being serviced.
5. Strip the unit for cleaning:
a. Remove the top plenum.
Note: For models with O₂ sensor, the sensor generates heat in order to prevent condensation around
it. Be careful not to touch the sensor probe.
Remove the front thumb screws.
Remove the thumb screws located at the back of the chamber.
Gently unhook the back of the plenum and pull it out from the chamber.
CO2 Incubator
32
Unplug the rear access port plug and remove the inlet filter.
Loosen the hose

clamp to remove
the filter
Plug the rubber cover at the left and right access port.
b. Remove the shelf system. See section 2.2.4 Shelves System Installation.
Items:
1. Back Pilaster
2. Front Pilaster
3. Railing
4. Tray or Shelf
Procedure:
1. Remove the tray or shelf.
2. Remove the railing by removing the front end
upward from the front pilaster and moving the
back end backward from the back pilaster.
3. The pilaster can be removed by unscrewing
the thumb screws holding the pilasters.
c. Remove the sparger and tubing (for suppressed O₂ model) and the humidity pan.
6. Start the cleaning process:
Note: When cleaning and disinfecting, always observe the safety instructions and hygiene regulations.
a. Thoroughly remove dirt and residues from the surfaces of the work space using the mild soap
solution and a sponge, or spray an appropriate disinfectant onto work space surfaces and all
dismantled parts. Allow disinfectant to react as specified by manufacturer.
Note: Do not spray disinfectant directly onto sensors, on the control panel, and on surfaces near the
electrical panel to prevent damage to electrical parts. Use a cloth soaked with disinfectant to wipe the
control panel and the exterior body.
CelCulture®
33
b. Rinse the soap solution or disinfectant twice using the distilled water in a wash bottle. If the
part is hard to rinse with a wash bottle, use a wet sponge to remove the soap.
c. Dry all rinsed part with a clean lint-free cloth or paper towel.
d. Wipe the interior components and exterior surfaces with 70% ethanol then allow to dry.
7. Re-assemble the unit by reversing step 5. Ensure that the equipment has completely dried before
commencing normal operation.
Note: Surface of a CO₂ incubator with copper chamber (CCL-Cu) is prone to dirt & fingerprint marks (once touched with
hands) and has a tendency to give a greenish appearance when oxidation occurs (upon contact with carbon dioxide).
Clean regularly with lemon soap, vinegar or baking soda. About 1 tablespoon of salt per 1 cup of vinegar can be also
used to remove tarnishes. If the surface or the humidity pan turned green, use sand paper. Contact your Local Distributor
for the available passivation solution to use.
5.2 Decontamination
Decontamination in CelCulture® CO₂ incubator uses 90°C moist heat to kill organisms that commonly
contaminate workspace including all installed components. The entire program run of the decontamination
routine takes approximately 15 hours (overnight process). The chamber is cool and dry at the end of the cycle
(applicable to CCL-170L/240L units only). No further wipe down is necessary.
Note: CCL-50L unit does not have a decon pump to dry the chamber after the decontamination cycle. Further wipe down
of the chamber is required for CCL-50L models since condensation will normally occur after the cycle.
Note: The incubator must not be used to decontaminate objects other than the incubator and its
components.
5.2.1 Decontamination Procedure

• Press the DECON button. And follow the instruction in the
main display.
Back
Rear access plug
• All sensors are located in the top duct (refer to the

drawing at the left).
1. Blower – no need to remove
2. RH Sensor (optional) – no need to remove
3. O2 Sensor (if installed) – no need to remove
4. IR CO2 Sensor – no need to remove
5. Temperature Sensor – no need to remove
• Remove the left rear access port plug (for CCL-
170L/240L units only); no need to remove the rear
access port plug in CCL-050L unit).
• Keep the ULPA filter inside the incubator during decontamination (for CCL-170L/CCL-240L units only).
• CCL-50L unit has no top duct, no ULPA filter installed, and has a small blower fan for forced convection.
No need to remove blower during decontamination.
CO2 Incubator
34
• Replace water from the humidity pan with distilled water according to the following volumes:
Size Water volume for decon (ml)

CCL-050L 200
CCL-170L 400
CCL-240L 400
• Once the decontamination is done, fill in the humidity pan (400 ml water for CCL-050L and 800 ml for
CCL-170/240L,) and reinstall the rear access plug (CCL-170/240L).
CelCulture®
35
Chapter 6 - Troubleshooting
Under no circumstances should the unit be disassembled, repaired, or converted by unqualified people. Failure
to follow these instructions may cause personal injury and/or loss of property due to malfunctions, electric
shocks or fire.
Should the unit malfunction, turn off the incubator and disconnect the power supply. Continuing to operate the
unit when it shows signs of malfunctioning may cause electric shocks or fire.
The following troubleshooting guidelines address some of the basic problems that can be solved by the user or
operator. However, if the problem persists, please contact your local distributor.
Problem 1: Unit will not turn on

Possible Cause Corrective Actions
Power failure • Is the cord set connected to the power inlet at the rear of unit as defined in the installation
section of the manual?
• Is there power at the electrical outlet which the unit is plugged into?
• Use voltmeter or test pen to check power on the electrical socket.
• Is the unit’s power switch turned on?
• Check whether power cord has been connected properly to the wall/building socket and also
into the incubator.
• Check whether the power cord is giving power, measure the AC voltage between the live and
the neutral terminal of the cord by using a voltmeter. If voltage is not within +/-2% of the
socket voltage, replace the power cord.
• Check the fuse of the power cord.
Problem 2: CO₂ level is below or above CO₂ set-point

Unit is not connected to the • Check gas hose connection at the rear of unit to the CO₂ source as defined in the installation
pressure regulated CO₂ source. section of the manual.
CO₂ inlet pressure not regulated • The CO₂ inlet gas pressure should be regulated to approximately 15 psig.
correctly.
CO₂ gas tank is empty. • Replace CO₂ gas tank.
In-line filter is dirty / saturated. • Replace in-line filter.
Gas hose is leaking. • If hose is leaking, check the pressure regulator to ensure CO₂ inlet pressure is not more than
15 psig.
• Check for leakage at hose connection.
Gas hose is dirty or obstructed. • Check physically for dirt accumulation or obstruction, clean or replace it.
CO₂ set-point was recently • Check CO₂ set-point. Allow 2 hours for the unit to stabilize after adjustment of new set-point.
lowered or raised. Unit is still not • If the CO₂ set point is lowered, the outer and inner doors must be opened.
stabilized after the set-point was
changed.
Inner door opened for an • Close the door and let the unit stabilize.
extended period of time.
Access port stopper at the rear of • Ensure access port stopper and filter are installed.
the unit was removed or not
install.
Air leakage through inner door • Check installation of the gasket to ensure that it is properly secured to the unit and that there
gasket. is no gap.
• Check the joining ends of the gasket to make sure there is no gap. Use silicon sealant to seal
the joins if there is a gap.
• Check gasket profile is not deformed or torn. Otherwise, replace new door gasket.
Problem 3: Temperature is above or below temperature set-point

Temperature set-point was • Check temperature set-point. Allow 2 hours for the unit to stabilize after adjustment of new
recently lowered or raised. Unit is set-point.
still not stabilized after set-point • If the temperature was lowered, open the outer and inner doors.
was changed. • Lowering the temperature without opening the doors could lead to condensation.
• Check if the glass door latch is properly closed.
Inner door opened for an • Close the door and let the unit to stabilize.
extended period of time.
CO2 Incubator
36
Air leakage through Inner door • Check installation of the gasket to ensure that it is properly secured to the unit and that there
gasket. is no gap.
• Check the joining ends of the gasket to make there is no gap. Use silicon sealant to seal the
joins if there is a gap.
Defective mainboard • Check if the triac heater is not defective. Measure the voltage (ON/OFF testing under TEST
MODE). Must be <5 V when OFF.
Otherwise, replace the mainboard.
Small fan position too far to O2 • Move closer the small fan to O2 sensor housing
sensor
Problem 4: Humidity level is below standard humidity level (For units with RH display options installed)
There is no water or not enough • Ensure there is enough water in the humidity pan. For normal operating temperature,
water in the humidity pan. humidity pan should be half filled.
Problem 5: Unusually high gas consumption

Air leakage through inner door • Check installation of the gasket to ensure that it is properly secured to the unit and that there
gasket. is no gap.
• Check the joining ends of the gasket to make there is no gap. Use silicon sealant to seal the
joins if there is gap.
• Check gasket.
• Check if the glass door latch is properly closed.
• Ensure access port stopper and filter are installed.
CO₂ sensor not calibrated • Calibrate CO₂ sensor.
Defective CO₂ sensor • Contact local distributor for sensor replacement.
In-line filter is dirty / saturated. • Replace in-line filter.
Gas hose is leaking. • If the hose is leaking, check the pressure regulator to ensure CO₂ inlet pressure is not more
than 15 psig.
• Check for leakage at hose connection.
• Contact local distributor for new hose.
Inner door not properly closed. • Close the door and let the unit stabilize.
Air leakage through inner door • Check installation of the gasket ensure it is properly secure to the unit and that there is no
gasket. gap.
• Check the joining ends of the gasket to make sure there is no gap. Use silicon sealant to seal
the joins if there is a gap.
Problem 6: Circulation fan not running

Main door switch is faulty. • Ensure outer door is closed and pressing the door switch button.
• If the fan is still not working, contact local distributor.
Faulty fan motor. • Ensure outer door is closed and pressing the door switch button.
• If the fan is still not working, contact local distributor.
Problem 7: Condensation occurs in the chamber.

Outer door opened for an • Close the door and allow the unit to stabilize. Try to reduce the frequency of door opening.
extended period of time or
frequency of opening door is very
high.
Ambient conditions • Ensure sufficient wall clearance around the chamber.
• Ensure that the unit is not directly heated by sunlight.
• Check the ambient temperature level. Ideal ambient temperature is at least 20°C - 24°C (68°F
– 75°F). If ambient temperature is not in the range of 20°C - 24°C, the temperature of the unit
must be calibrated.
• Check the ambient humidity level. Ideal ambient humidity level is 60%. Ensure that ambient
humidity level is non-condensing.
• Door and perimeter heater must be adjusted according to ambient conditions. Contact local
distributor for heater adjustment.
Power interruption occurred • Take out the humidity pan and wipe the incubator surface
overnight • Set the temperature to 55°C and let the unit heat up for one hour
• Turn off the unit and open the incubator doors to allow the unit to cool down
• Turn on the unit and set the temperature back to the intended set point. Fill the humidity pan
with sterile distilled water according to the water level required.
CelCulture®
37
Problem 8: Water accumulated at the base of the chamber.

Condensed water from the cold • Ensure the humidity pan is pushed all the way to the back so that condensed water from the
spot did not drip back to the cold spot drips into humidity pan.
humidity pan.
Problem 9: Small fan is not working (for CCL-050_-_-_)

Defective fan • Ensure that the voltage supply from J-10 is 12.0 VDC.
Otherwise, replace the mainboard.
Problem 10: CO₂ Sensor error

Defective supply from J-10 • Relay optional supply from SMPS.
I-series mainboard • Contact local distributor.
Broken cable or cable connection • Contact local distributor
Defective CO₂ probe • Contact local distributor.

Defective CO₂ board • Contact local distributor.
Problem 11: O2 Sensor Error

Sensor needs calibration. • Contact local distributor.
Defective O2 sensor. • Contact local distributor.
Problem 12: Condensation occurs on the surface of inner door after decontamination process (For CCL-170
and CCL-240 units)
Sampling port on inner door is • Ensure the sampling port is capped.
not installed or capped.
Leakage through magnetic gasket • Check to make sure outer door can close fully and no gap.
on outer door. • If there is gap and cannot be closed properly, check that magnetic strength is not diminished
and PVC gasket profile is not deformed. Otherwise, replace with new magnetic gasket.
Decontamination pump failed. • Contact local distributor.
Filtered air exchange not working • Check whether filtered air exchange is installed.
or not installed. • There is a short tubing with a 1 μm filter attached to the access port located at the back of
the chamber.
• Check that filter is not saturated and tubing is not blocked.
• Remove the ULPA filter and make sure the tube is connected to the hole in the blower intake,
above where the ULPA filter is mounted.
• Check also if 2nd filter is not saturated and tubing is not blocked.
• Loosen the 2 thumb screws at the front of the top duct and lower the top duct carefully.
Check if the tubing is secured at the top of the chamber and tubing is not blocked. Re-secure
any loose tubing connection and close the top duct.
CO2 Incubator
38
Problem 13: Garbled or missing characters on LCD module

Display intermittently not stable • Press the MENU key twice and see if the display returns to normal. If it returns to normal and
is not occurring again then it is caused by intermittent electrical noise. Check the line cord
grounding.
LCD Module faulty. • If the garbled characters occur again on the same position and missing characters are exactly
same as before, then the LCD module is faulty. Contact local distributor.
Problem 14: Buzzer has no sound

Buzzer is disabled. • Go to ADMIN menu and choose AUDIBLE ALARM to enable the alarm.
Buzzer spoiled. • Contact local distributor.
Problem 15: “Memory 90% full” alarm/reminder displayed on LCD

Memory used already reached • Access factory menu:
90% of available storage data 1. Press DOWN button twice and then press SET button. Key in 0397 when needed.
capacity 2. Select the SHIPPING Mode and select install.
3. Press the MENU button to go back to normal mode.
• Unit re-activation after SHIPPING MODE selected:
1. Re-start the unit by turning it OFF and ON again.
2. When the welcome message appears, press SET button to adjust or set the temperature
and CO₂ set point.
3. When incubator setup is done, the unit will run a memory check and will display useable
memory (~ 2 MB).
Problem 16: Incubator have a severe condensation on glass door and inside chamber during cooling mode
Humidity pan inside chamber • Take out or dry out the humidity pan.
Problem 17: Incubator cannot reach temperature 8°C below ambient during cooling mode (CCL-P model)
Humidity pan filled with water • Remove humidity pan from the chamber, remove top duct during cooling mode.
Temperature ambient is too high. • Turn on air conditioning.
Room air conditioning is OFF • Set temperature ambient minus 6-8 above set point.
(If incubator set point is 15°C, so the ambient temperature should be 23°C-24°C)
• Go to TEST MODE, choose O2 VALVE2. Press ON and OFF to check the function.
Cooling control malfunction See the respond of temperature display decrease gradually,
• Contact local distributor if the temperature is not responding and decreasing
• Hear the sound of the cooling module running, otherwise contact local distributor.
Defective Peltier cooling module • Contact local distributor.
Problem 18: Incubator have severe condensation during cooling mode (CCL-P model)
Humidity pan filled with water • Remove and take out humidity pan when unit in cooling operation mode.
• Open glass door regularly to balance and clip the humidity.
• Contact local distributor if problem persists.
Problem 19: CO₂ is not injected, display shows 0.0% or % CO₂ display not responding
Defective CO₂ inject valve
• Change CO₂ valve, otherwise contact local distributor.
Defective valve channel from I-
• Change mainboard, otherwise contact local distributor.
series mainboard
Problem 20: CO₂ TANK LOW alarm displayed on display

System predicts gas depletion time • Change the CO₂ tank, key in to the system the new tank weight by accessing
and needs to change the tank ADMIN menu.
• To clear the alarm, key in to the system the new tank weight (31 kg) by accessing ADMIN
menu
CelCulture®
39
Problem 21: O₂ is not injected, display shows 0.0% or % O₂ display not responding
Defective N₂ inject valve
• Change N₂ valve, otherwise contact local distributor.
Defective valve channel from I-
• Change mainboard, otherwise contact local distributor.
series mainboard
The tubing connected to N₂ is
• Do correction for tubing
bent
Problem 22: ULPA Filter reminder

• Change into new filter and key in to the system the new filter.
System predicts filter run hour time
• To clear the alarm, key in to the system new ULPA filter by access ADMIN menu.
and needs to change the filter
SET ULPA REMINDER—Key in 12 months
Problem 23: UV Lamp does not turn on

Door is open • Make sure that the door is fully closed and that the door is firmly pressed onto the door
switch.
Busted UV Lamp • Contact local distributor.
Problem 24: UV Lamp is always on

Defective UV relay • Replace mainboard
Problem 25: Outer door is not aligned to the main body

Gap in the upper hinge • Place a foam between the lower part of main door and its main body, then push the outer
door at the upper portion.
Gap in the lower hinge • Place a foam between the upper part of main door and its main body, then push the outer
door at the lower portion.
Defective door hinge • Replace old ceramic hinges (now obsolete).
• Contact local distributor for the new stainless-steel hinges.
• When replacing with new hinges, put foam at the bottom of the door assuring that outer door
is aligned to main body.
CO2 Incubator
40
Chapter 7 – Technical Specifications

MODEL CCL-050_-_ CCL-170_-_ CCL-240_-_
Temperature
Temp. Control Method Direct Heat and Air Jacket Using Microcontroller PI
Ambient Temp. Range 18°C - 30°C (64.4°F – 86°F)
Temp. Control Range, °C Ambient +7 to 60
Temp. Uniformity, °C* <± 0.5
Temp. Accuracy, °C* <± 0.1
Temp. Recovery Time**
(after 30 seconds door opening, 98% from ≤5 minutes ≤5 minutes ≤6 minutes
initial value)
CO₂
CO₂ Control System Microcontroller PI
CO₂ Control Range, % CO₂ 0-19.5
CO₂ Accuracy, % CO₂ *** ± 0.1
CO₂ Sensor Infrared (IR) CO2 Sensor
Standard unit: ≤6
CO₂ Recovery Time****
minutes Standard unit: ≤5 minutes Standard unit: ≤6 minutes
(after 30 seconds door opening, 98% from
Suppressed O2 model: Suppressed O2 model: ≤6 mins. Suppressed O2 model: ≤10 mins.
initial value) ≤8 mins.
O2 (For Models with Suppressed O2 Control)
O2 Control System Microcontroller PI
O2 Control Range, % O2 1-18
O2 Accuracy % O2***** ± 0.1
O2 Sensor Zirconium dioxide sensor type
O₂ Recovery Time******
At 5.0% O₂ by volume: At 5.0% O₂ by volume: ≤10
(after 30 seconds door opening, 98% from At 5.0% O₂ by volume: ≤12 mins.
≤10 mins. mins.
initial value)
Humidity
Humidification Method Humidity Pan
Humidity Range*******, % RH 85-90
Physical Construction
Interior Volume 50 L (1.8 ft3) 170 L (6 ft3) 240 L (8.5 ft3)
External Dimensions 500 x 500 x 655 mm 660 x 660 x 900 mm 750 x 770 x 900 mm
(W x D x H) (19.7” x 19.7” x 25.8”) (26.0” x 26.0” x 35.4”) (29.5” x 30.3” x 35.4”)
Internal Dimensions 345 x 375 x 388 mm 505 x 535 x 633 mm 595 x 640 x 633 mm
(W x D x H) (13.6” x 14.8” x 15.3”) (19.9” x 21.1” x 24.9”) (23.4” x 25.2” x 24.9”)
Main Body Electrogalvanized steel with ISOCIDE™ antimicrobial coating
Interior Material Stainless steel, type 304
Number of Shelves 3 4 4
Chamber Maximum Number of
4 7 7
Construction Shelves
300 x 335 mm 465 x 470 mm 550 x 560 mm
Shelves Area (W x D)
(11.8” x 13.2”) (18.3” x 18.5”) (21.7” x 22.0”)
Maximum Load per 4 kg/shelf 11 kg/shelf 15 kg/shelf
Shelf (8.8 lbs/shelf) (24.3 lbs/shelf) (33.1 lbs/shelf)
Nominal Power at
Electrical 40.9 W 42.2 W 42.2 W
370C
Configuration Max. Power
(110-130 VAC, 675.5 W 1184.3 W 1727.9 W
Consumption
50/60 Hz)
Full Load Amps 5.3 A 9.2 A 13.4 A
Nominal Power at
Electrical 40.9 W 42.2 W 42.2 W
370C
Configuration Max. Power
(220-240 VAC, 598.8 W 1008.9 W 1270 W
Consumption
50/60 Hz)
Full Load Amps 2.5 A 4.2 A 6.5 A
Net Weight 52 kg (114.6 lbs) 101 kg (222.7 lbs) 121 kg (266.8 lbs)
Shipping Weight (W x D x H) 70 kg (154.3 lbs) 120 kg (264.6 lbs) 155 kg (341.7 lbs)
660 x 650 x 900 mm 850 x 720 x 1120 mm 850 x 850 x 1120 mm
Shipping Dimensions (W x D x H)
(26.0” x 25.6” x 35.4”) (33.5” x 28.3” x 44.1”) (33.5” x 33.5” x 44.1”)
Shipping Volume 0.39 m3 (13.7 ft3) 0.70 m3 (24.85 ft3) 0.79 m3 (28.03 ft3)
CelCulture®
41
Contamination Control
1) Main body is electro-galvanized steel with ISOCIDE™ antimicrobial coating;
2) 90˚C moist heat OVERNIGHT decontamination cycle (HPA validated);
3) ULPA filter********
Contamination Control Methods
4) 0.2-micron inlet filter for gas inputs;
5) 1-micron air circulation filter
6) Optional copper chamber and shelves
All data recorded is specified for standard models with unloaded chambers and tested under optimum factory setting conditions of
23°C and 60% ambient humidity.
*Results are achieved when tested at 37 0C as set point. Results may vary if set point changes and calibration is needed.
**For temperature not exceeding 37.2°C.
***Results are achieved when tested at 5% CO2 as set point. Results may vary if set point changes and calibration is needed.
****For CO₂ level not exceeding 5.2%.
*****Results are achieved when tested at 5% O2 as set point. Results may vary if set point changes and calibration is needed.
****** For O₂ level not lower than 4.8%.
******* Esco does not guarantee condensation-free chamber at humidity level higher than 90%.
******** Not available for 50 L models.
CO2 Incubator
APPENDIX
APPENDIX A: LOG RECORD
Thermostatic Unit : __________________________________________________________
Serial Number : __________________________________________________________
Person in-charge : __________________________________________________________
1. This log record should be used by the operator to record any new agent/virus/bacteria/protist that has
been introduced to the unit during its operation, problems encountered, etc.
2. Any decontamination procedure performed by either the user or the technician should be recorded
down as well.
3. Please also record any major maintenance procedure performed by the service technician, for example:
parts replacement, recertification, etc.
Date Event User Signature Supervisor Signature
In case of emergencies, please call:

Name : _______________________________________________________________
Cell Phone No. : _______________________________________________________________
E-mail : _______________________________________________________________

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Thank you for purchasing this Esco CO2 Incubator.

Please read this manual thoroughly to familiarize

Esco provides many other resources at

For Technical Service, contact

REST OF THE WORLD

1 Chapter 1 – Product Information

16 Chapter 3 – Control System and Operation

29 Chapter 4 – General Maintenance

31 Chapter 5 – Cleaning and Decontamination

40 Chapter 7 – Technical Specifications

MANUAL REVISION HISTORY

Updated specifications for - -

Removed Instructions for Proper

Warranty Terms and Conditions

This limited warranty does not cover:

Esco encourages all users to register their equipment online at www.escoglobal.com/warranty_registrations.

Technical Support, Warranty Service Contacts

Product Appendix, Warranty Listing

CO2 Incubators 2 years limited

CelCulture CO₂ Incubator (Stainless Steel Chamber with Flat Door)

CelCulture CO₂ Incubator (for IVF Application)

CelCulture CO₂ Incubator (100% Copper Chamber)

CelCulture CO₂ Incubator (with Peltier Cooling Mode)

CelCulture CO₂ Incubator with UV Lamp (100% Copper Chamber)

CelCulture CO₂ Incubator with UV Lamp (with Peltier Cooling Mode)

• Any adjustment, modification or maintenance to this equipment must be undertaken by qualified

4. European Union Directive on WEEE and RoHS

• Directive 2002/95/EC on Restriction on the use of Hazardous Substances (RoHS)

Caution: Electrical Hazard

Caution: Hot Surface

We, Esco Micro Pte.Ltd.

declare on our sole responsibility that the product:

in accordance with the following directives:

Chapter 1 - Product Information

Model – model of the unit

1.3 Quick View

1. Electrical panel cover

*CCL-050_-_-IVF units have 2 sealed inner glass doors

1.3.2 Front Quick View (CCL-170_-_-_, CCL-240_-_-_)

1. Electrical panel cover

*CCL-170_-_-IVF units have 4 sealed inner glass doors

1.3.3 Front Quick View (CCL-170_-_-UV, CCL-170_-_-P-UV, CCL-240_-_-UV, CCL-240_-_-P-UV)

1. Electrical panel cover

1.3.4 Back Quick View (CCL-050_-_-_, CCL-170_-_-_, CCL-240_-_-_, CCL-170_-_-UV, CCL-240_-

*not present in 50L models

1.3.5 Back Quick View (CCL-170_-_-P, CCL-240_-_-P, CCL-170_-_-P-UV, CCL-240_-_-P-UV)

2.1.1 Location Requirements

2.1.1.1 Relocating the Unit

2.1.2 Environmental Requirements

Location Indoor use only

2.1.3 Power Requirements

2.2.1 Unit Leveling

2.2.2 Unit Stacking

2.2.3 ULPA Filter Installation (For 170L & 240L)

2.2.3.1 Installing ULPA Filter from Old to Latest Configuration

CO₂ Part number of

To install the new simplified conversion kit

1. Prepare the simplified conversion kit.

2.2.4 Shelves System Installation