BX 43

INSTRUCTIONS
SYSTEM MICROSCOPE
BX43
This instruction manual is for the Olympus System Microscope Model BX43.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating the
microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
AX7851
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In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment,
this symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in your
country.
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user's authority to operate the equipment.

BX43
CONTENTS
Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble
the microscope yourself, please read section 10, “ASSEMBLY” (pages 33 to 37) carefully.
IMPORTANT -- Be sure to read this section for safe use of the equipment. -- 1-3
1 MODULE NOMENCLATURE 4
2 CONTROLS 5-7
3 FLOW OF OBSERVATION 8,9
4 SIMPLIFIED OBSERVATION PROCEDURE 10-12
4-1 Basic Operation (Until Observation of Specimen)...................................................................... 10,11

4-2 Microscope Adjustments (How to improve the Observed image)...................... 11,12
1 Adjusting the Interpupillary 2 Adjusting the Left and Right
Distance.................................................................................11 Focusing................................................................................11
3 Adjusting the Centering..................................12 4 Adjusting the Contrast......................................12
5 USING THE CONTROLS 13-25
5-1 Base...............................................................................................................................................................................................................13-15
1 Adjusting the Brightness...............................13 2 Setting the LIM Brightness..........................13
3 Using the Filters (Halogen Bulb
Operation Only)...........................................................14
5-2 Focusing Block................................................................................................................................................................................ 16,17

1 Focusing Controls...................................................16 2 Adjusting the Focus..............................................16
3 Replacing the Fine Adjustment 4 Adjusting the Coarse Adjustment
Knob............................................................................................. 17 Knob Tension.................................................................. 17
5 Pre-focusing Lever................................................... 17
5-3 Stage............................................................................................................................................................................................................18-24
1 Placing the Specimen.......................................18 2 Using the Auxiliary Slide Holder.........18
3 Adjusting the X- and Y-Axis 4 Rotating the Stage................................................ 20
Knob Tension.................................................................19
5 Adjusting the Stage Height...................... 20
5-4 Observation Tube................................................................................................................................................................... 21-24

1 Adjusting the Diopter........................................ 21 2 Using the Eye Shades..................................... 21
3 Using the Eyepiece Micrometer 4 Selecting the Light Path of the
Disk............................................................................................. 22 Trinocular Tube......................................................... 22
5 Adjusting the Tilt...................................................... 23 6 Using Eyepieces Incorporating a
Micrometer....................................................................... 24
5-5 Condenser.................................................................................................................................................................................................... 24
1 Compatibility of Objectives and
Condensers.................................................................... 24

5-6 Immersion Objectives.................................................................................................................................................................. 25
1 Using Immersion Objectives.................. 25
5-7 Objectives with Correction Collar............................................................................................................................... 25
6 CAMERA RECORDING 26
1 System Chart.......................................................... 26 2 Selecting the Camera Adapter

Magnification......................................................... 26
7 TROUBLESHOOTING GUIDE 27-29
8 SPECIFICATIONS 30,31
9 OPTICAL CHARACTERISTICS (UIS2 Series) 32
10 ASSEMBLY -- See this section for the replacement of the light bulb. -- 33-37
11 HALOGEN LAMP SOCKET INSPECTION SHEET 38
PROPER SELECTION OF THE POWER SUPPLY CORD........................................................... 39,40

BX43
IMPORTANT
SAFETY PRECAUTIONS (Fig. 1)
1. After the equipment has been used in an observation of a specimen that

is accompanied with a potential of infection, clean the parts coming in
contact with the specimen to prevent infection.
· Moving this product is accompanied with the risk of dropping the
specimen. Be sure to remove the specimen before moving this
product.
· In case the specimen is damaged by erroneous operation, promptly
take the infection prevention measures.
· Follow the procedures described in Chapter “Getting Ready” (see on
page 2) prior to using the accessories of this product. Otherwise, the
stability of the device will be lost and the dropped specimen will cause
the possibility of infection.
2. Install the microscope on a sturdy, level table or bench.
3. Be careful not to tilt the microscope too much. Otherwise, the mobile
parts such as the light path selector knob may move in unintended
directions.
4. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual.
If the proper power cord is not used, product safety performance cannot
be warranted.
5. Always ensure that the grounding terminal of the microscope and that of the
wall outlet are properly connected. If the equipment is not grounded, Olympus
can no longer warrant the electrical safety performance of the equipment.
Operation Using the LED Lamp

Safety Note on LED (Light Emitting Diode)
The LED incorporated in the LED lamp housing is a class 1 LED
product as defined in the applicable standard. The light emitted by LED
is basically safe, but do not look at the illumination light directly for an
extended period to prevent your eye from being injured
CLASS 1 LED PRODUCT
Operation Using the Halogen Bulb

1. To avoid potential shock hazards and burns when replacing the halogen
bulb, set the main switch of the TL4 power supply unit to “ ” (OFF) and
allow the lamp socket @ and bulb to cool before touching them.
Designated bulb 6V30WHAL (PHILIPS 5761)
CAUTION Always use the designated lamp bulb. Using other bulb may
1 cause malfunction.
2. If the lamp cable or power cord comes in contact with the lamp socket
Fig. 1
or its surroundings, the cable or cord may be melt by the heat, causing
an electric shock or fire hazard. To prevent this, distribute the lamp cable
and power cord at enough distances from the lamp socket.
3. The standard service life of the lamp housing is eight (8) years of use or
20,000 hours of total power ON period, whichever is the shorter period.
For details, see Inspection Sheet on page 38.
1
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol Explanation
Indicates that the surface becomes hot, and should not be touched with bare hands.
Indicates a non-specific general hazard. Follow the description given after this symbol or in
instruction manual.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Caution engraving/label
Caution engraving and label are placed at parts where special precaution is required when handling and using the
microscope. Always heed the cautions.
Caution engraving Halogen lamp socket

position [Caution against high temperature]
Caution label Rear panel

position [Caution against high temperature]
If a caution engraving or label is dirty or peeled off, contact Olympus for the replacement or other inquiry.
1 Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact. Also note that the microscope
does not have a waterproof construction.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations. (For the operating conditions,
refer to section 8, “SPECIFICATIONS”.)
3. When moving the microscope, remove the observation and specimen
and carefully carry the microscope by holding the grasping part on the
rear of the arm and the front part of the base as shown in Fig. 2.
CAUTION To prevent damage to the microscope, never hold it by the stage
Fig. 2 or coarse/fine adjustment knob.
· Even when moving the microscope along the desktop surface, be sure
to remove the specimen in advance.
· When the microscope needs to be packaged for forwarding to a remote
location, contact Olympus.
4. When the accessories of this product are used, make sure that the system
height does not exceed 1 meter. Otherwise, the stability of the device will
be lost.
2
BX43
2 Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
alcohol.
CAUTION · Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical
equipment that is being switched on or off, which could cause ignition of a fire.
Also remember to always use it only in a well-ventilated room.
· Set the main switch to “ ” (OFF) and wait for the lamp housing to cool down sufficiently before proceeding
to maintenance.
2. Do not use organic solvents to clean the microscope components other than the glass components. To clean them,
use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope except for the parts that are specified to be disassembled in this manual,
as this could result in malfunction or reduced performance.
4. After using the microscope, set the main switch to “ ” (OFF), (wait for the lamp socket to cool down sufficiently if the
halogen bulb has been used,) and keep it covered with a dust cover during storage.
5. Before disposing of this product, be sure to follow the regulations and rules of your local government.
3 Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury or damage to the equipment or other property. It may also be used
to alert against unsafe practices.
} : Indicates commentary (for ease of operation and maintenance).
4 Intended use
This instrument has been designed to be used to observe magnified images of specimens in various routine work and
research applications.
Do not use this instrument for any purpose other than its intended use.
This instrument is considered a medical device in the following countries: US, and China.
This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic
medical devices. CE marking means the conformity to the directive.
3
1 MODULE NOMENCLATURE
} The modules mentioned below show only the typical product names. As there are some products that are not mentioned
but also applicable to this microscope, check the latest catalogues or consult Olympus.
For the products marked “ * ”, also read their instruction manuals.
Observation tube
Intermediate attachment
· U-BI30-2
· U-TBI-3 · U-TRA
· U-TR30-2 · U-CA
· U-TTLBI · U-DO3
Eyepieces
· WHN10X Arm (or vertical illuminator)
· WHN10X-H · BX3-ARM
· BX3-URA*
· BX3-RFAS*
Lamp housing
· U-LHLEDC
· U-LS30-5
Revolving nosepiece
· BX43-5RES
· U-5RE-2
· U-D6RE
Slide holder
· U-HLST-4
· U-HD-4
· U-HLDT-4
Stage
· U-SVRB-4
· U-SVRO
Condenser
· U-LC
· U-SC3
· U-AC2
4
BX43
2 CONTROLS
} If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 33 to 37).
Interpupillary distance adjustment

scale (Page 22)
Condenser height adjustment

knob (Page 12)
Diopter adjustment ring

(Page 22)
Slide holder clamping lever

(Page 19)
Aperture iris diaphragm ring

(Page 12)
Allen screwdriver
holder
Condenser centering screws

(Page 12)
Coarse adjustment
Filter mount (Page 15) knob (Page 18)
Field iris diaphragm ring

(Page 12)
Y-axis knob (Page 20)
X-axis knob (Page 20)
Coarse adjustment tension

adjustment ring (Page 18)
5
Main switch (Page 10)
I : ON
: OFF
Pre-focusing lever
(Page 16)
LIM setting switch

Fine adjustment knob (Page 13)
(Page 16)
LED brightness adjustment

knob (Page 13)
LIM indicator LIM ON-OFF switch

ON: Lit (Green) (Page 13)
OFF: Extinguished
6
BX43
Low Magnification Condenser Swing-out Condenser

U-LC U-SC3
Auxiliary lens mounting Top lens
tool (for BX53)
Top lens swing-out
knob
Aperture iris diaphragm

ring (Page 12)
Auxiliary lens
Condenser mount
dovetail
* The auxiliary lens is mounted on the lamp adapter when the U-LC is used. (Page 34)
<<Modules for halogen lamp operation>>

LS30 Adapter Halogen Lamp Socket
U-LS30ADP U-LS30-5
Guide pins
Guide pin holes
Lamp cable
Power Supply Unit

TL4
Lamp socket connector
Brightness control
knob
Main switch
I : ON : OFF
7
3 FLOW OF OBSERVATION
} When the LED lamp is used and the LIM is set, the LED brightness adjustment knob is defeated.
} When the halogen bulb is used, set the LBD filter in the filter mount on the base (or in the U-FC filter cassette).
(Controls Used) (Page)
LED lamp Halogen bulb

@Main switch 3Main switch
Set the main switch to “ I ” (ON) and adjust
2LED brightness |Brightness (P. 13)
the brightness.
adjustment knob* adjustment knob
Select the light path (trinocular tube). 5Light path selector knob (P. 22)
Place the specimen on the stage. 6Slide holder (P. 19)

7X-/Y-axis knobs (P. 20)
Engage the 10X objective in the light path. 8Revolving nosepiece
Bring the specimen in focus. 9Coarse/fine adjustment knobs (P. 17)
aBinocular tube (P. 21)

Adjust the interpupillary distance.
bDiopter adjustment ring (P. 21)
Adjust the diopter.
cCondenser height adjustment knob (P. 24)
Adjust the light axis.
dCondenser centering knob (P. 12)
Adjust the aperture iris and field iris diaphragms. eAperture iris diaphragm ring (P. 12)
fField iris diaphragm ring (P. 12)
Engage the desired objective in the light path

8Revolving nosepiece
and bring the specimen in focus.
Insert the required filters. gFilter mount (P. 14)
Adjust the brightness. 2LED brightness |Brightness (P. 13)

adjustment knob* adjustment knob
Start observation.
* The LED brightness adjustment knob is defeated when the LIM is set (see page 34).
8
BX43
a
5 (trinocular tube only)
b
8
7
d
g
2
9 4
} Copy the observation procedure pages on separate sheets and post it near your microscope.
9
4 SIMPLIFIED OBSERVATION PROCEDURE
4-1 Basic Operation (Until Observation of Specimen)
This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed
operating procedure of each control, please read the description page specified below.
1 Press the main switch of the microscope frame to “ I ” (ON).
Fig. 3
2 Rotate the knob to adjust the lamp brightness. (Details: Page 13)
Fig. 4
4 3 Rotate the knob to lower the stage. (Details: Page 18)
4 Open the clamping lever of the slide holder and place the specimen
slide. (Details: Page 18)
Fig. 5
10
BX43
5 Rotate the revolving nosepiece to engage the 10X objective in the

light path.
6 Rotate the coarse and fine adjustment knobs to bring the specimen
5 into focus. (Details: Page 17)
6
7 7 Rotate the stage knob to adjust the observation position.
Now you can observe the magnified image of the specimen. To

Fig. 6 improve the observation further, read section 4-2, “Microscope
Adjustments” below.
4-2 Microscope Adjustments (How to Improve the Observed Image)

Adjust the microscope as described below to improve the observed image.
1 Adjusting the Interpupillary Distance
1 While looking through the eyepieces, adjust for binocular vision until
the left and right fields of view coincide completely.
} Note your interpupillary distance so that it can be quickly duplicated.
Fig. 7
Adjusting the Left and Right Focusing

2 2
(diopter adjustment)
1 Looking through the eyepiece without the diopter adjustment ring,

rotate the coarse and fine adjustment knobs to bring the specimen
into focus.
2 Looking through the eyepiece with the diopter adjustment ring, turn
only the diopter adjustment ring to focus on the specimen.
} If the diopter adjustment rings are provided on both the left and
Fig. 8 right sides, see “Adjusting the Diopter” on page 21.
The diopter adjustment rings may be provided on either the eye-
pieces or the observation tube.
11
3 Adjusting the Centering
7
4 6
5
3
8 9
1 Place the specimen.
2 Rotate the revolving nosepiece to select the 10X objective.
3 Rotate the knobs to bring the specimen into focus.
4 Rotate the knob to raise the condenser to its upper limit.

Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field
5 of view.
6 Rotate the knobs to focus on the field iris diaphragm image (A in Fig. 9).
7 Turn the two condenser centering screws to move the iris diaphragm image to the center of the field of view (B in Fig. 9).
8 Gradually open the field iris diaphragm until its image is inscribed in the field of view (C in Fig. 9).
9 Open the field diaphragm slightly until its image circumscribes the field of view slightly (D in Fig. 9).
} Be careful not to rotate the condenser clamping screw, located behind the centering knobs, by mistake.
} When using a condenser with top lens such as the U-SC3, engage the top lens in the light path before proceeding to
the centering adjustment.
A B C D
Fig. 9 Movement of Field Iris Diaphragm Image
4 Adjusting the Contrast
Aperture iris
diaphragm image 70-80%
30-20%
Objective pupil
2
N.A. scale 1
1 Set the condenser aperture iris diaphragm ring so that the condenser NA scale position indicates 70% of the NA of the
objective in use. After this, observe the image and fine adjust the aperture iris diaphragm to the desired condition.
2 The image of the aperture iris diaphragm can be viewed by removing the eyepieces and looking into the observation
tube. Adjust so that the image looks approximately like that shown in the figure above.
12
BX43
5 USING THE CONTROLS
5-1 Base
1 Adjusting the Brightness (Fig. 10)
1. When the LED lamp is used for observation, turn the LED brightness
adjustment knob @ clockwise to make illumination brighter.
2. When the halogen bulb is used for observation, adjust the brightness
adjustment knob 2 of the TL4 power supply unit.
Fig. 10
Fig. 11
2 Setting the LIM Brightness (Fig. 12)
} LIM stands for Light Intensity Manager. It is effective when the BX43-5RES
2 revolving nosepiece with sensor and the U-LHLEDC LED lamp housing
are used.
1 } The desired brightness can be set per objective. Once the brightness for
the objectives is set, selecting an objective adjusts the LED brightness
3 automatically provided that the LIM ON-OFF switch @ is set to “ON” (which
is indicated by the lighting of the LIM indicator 2).
4 1. Press the LIM ON-OFF switch @ to “OFF” (so that the LIM indicator 2 is
extinguished).
Fig. 12
2. Engage a low-power objective in the light path and bring the specimen
in focus.
3. Rotate the brightness adjustment knob 3 for optimum brightness for
observation.
4. Press the LIM setting switch | to enter the current brightness.
5. Set the optimum brightness for the mounted objectives by repeating the
above for each of them.
To modify the LIM setting:

Engage the objective you want to set the new LIM setting in the light path,
set the LIM ON-OFF switch to “OFF,” and perform steps 3 and 4 above.
13
3 Using the Filters (Halogen Bulb Operation Only)
} You can place a filter in the light path with either method.
· Place a filter on the filter mount on the base and engage in the light path.
(Page 14)
· Insert a filter in the U-FC filter cassette, attach it on the filter mount and
engage it in the light path by sliding the filter level. (Page 14)
Mounting a Single Filter (Fig. 13)

A filter with a diameter of 45 mm can be placed on the filter mount on
Fig. 13 the base. If you need to place multiple filters, please purchase the U-FC
filter cassette.
CAUTION Even when a filter cassette is used, a filter with thickness of no
more than 3 mm can still be placed on the filter mount.
Using the Filter Cassette (Figs. 14 to 17)

Loading Filters into Filter Cassette
} The filter cassette accommodates filters with a diameter of 45 mm and
thickness of 2.7 mm or less.
} The filter cassette has two filter levels on the right side and one on the
1 left side.
} When the filter cassette is used, the ECO mode cannot be used because
the human sensor is hidden by the filter cassette.
1. Move all filter levers to the OUT position except for the one belonging to
the slot into which the filter is to be inserted.
Fig. 14
2. Slide lever @ to the IN position. Make sure that it clicks securely into
place. (Fig. 14)
3. Holding the lever in the position shown in Fig. 14, put the filter into the
3 cassette by inserting it in the direction indicated by the arrow.
4. Insert the other two filters in the same manner.
2 Mounting the Filter Cassette
4 1. Fully loosen the filter cassette clamping screw 2. (Fig. 15)
2. Align the key 3 on the bottom surface of the filter cassette with the
positioning slot | on the filter mount, then snap the filter cassette into
place from above.
5 3. Rotate the filter cassette to align its sides with the base. (Fig. 16)
4. Align the clamping screw 2 with the positioning hole 5 on the filter
Fig. 15 mount, then tighten the screw to fasten the filter cassette.
CAUTION When the filter cassette is installed, the condenser may hit it
when the stage is lowered. Therefore, exercise caution when
lowering the stage with the filter cassette installed.
14
BX43
Using the Filter Cassette (Fig. 17)
Usable Filters Applications

45LBD-IF* Color balancing filter
45-ND6, 45-ND25 Neutral density filter
45G-530, 43IF550-W45* Green
B&W contrast filters
45O-560 Orange
Up to three of the above filters can be loaded in the filter cassette. Moving
Fig. 16 the levers 6 on the left and right sides of the cassette to the IN position
brings the corresponding filter into the light path.
* When an IF (interference) type filter is used, flare and ghost may be
observed. These phenomena may be reduced by adding ND filter(s) to
the IF filter.
} The LBD filter is not required when the LED lamp is used.
What is LBD filter?

The color of the sun at the noon of a clear day is called the daylight
color, which looks white to human eyes. On the other hand, the
halogen bulb used as the light source of this microscope does not
6 emit the daylight-color light. so the LBD filter is engaged in the light
path to convert the color of halogen bulb light into daylight. In general,
Fig. 17 engaging the LBD filter makes it possible to observe specimens in
natural color tones.
15
5-2 Focusing Block
1 Focusing Controls
The focusing block consists of the controls listed in the following table,
1 which also describes the functions of the controls.
No. Name Function
2
1 Coarse adjustment Moves the focus position widely.
knob
5 2 Fine adjustment dial Fine adjusts the focus position.
3 Fine adjustment Fine adjusts the focus position.
Fig. 18 knob This knob can be attached to the fine
adjustment dial on either the left or right
side. (When the microscope is shipped,
it is attached to the right-side dial.)
4 Pre-focusing lever Sets the upper limit of the stage position
(to prevent contact between the speci-
men and objective).
4 5 Tension adjustment Adjusts the force required to rotate the
ring coarse adjustment knob.
3
1
Fig. 19
2 Adjusting the Focus

With both the coarse adjustment knob @ and fine adjustment knob 2,
rotating the knob in the direction of the arrow raises the stage (lets the
specimen approach the objective).
2
1
Fig. 20
16
BX43
3 Replacing the Fine Adjustment Knob (Fig. 21)

4
CAUTION The fine adjustment knob has been attached on the right side
at the factory.
} The fine adjustment knob is designed detachable to prevent interference
with hand during manipulation of the X-and Y-axis knobs.
Usually attach the knob on the opposite side to the X- and Y-axis
3 2 1 knobs.
1. Using the Allen screwdriver, loosen the clamping screw @ and remove
the fine adjustment knob 2.
Fig. 21 2. Remove the seal from the fine adjustment knob screw hole on the other
side and attach the knob by reversing the removal procedure.
3. Attach a provided seal on the screw hole | of the removed fine adjustment
knob 3.
} The fine adjustment dial 3 can be operated with your fingertip or finger
surface at the same time as manipulating the X- and Y-axis knobs.
1 4 Adjusting the Coarse Adjustment Knob Tension (Fig. 22)
CAUTION Adjust the coarse adjustment knob tension using the tension
adjustment ring @.
The coarse adjustment knob tension is preadjusted for easy use. However,
if desired, you can change the tension using the tension adjustment ring
@. Turning the ring in the direction of the arrow increases tension, and
vice versa.
The tension is too low if the stage drops by itself of focus is quickly lost
after adjustment with the fine adjustment knob. In this case, turn the ring
Fig. 22 Fig. 22 in the direction of the arrow to increase tension.
5 Pre-focusing Lever (Fig. 23)
} The pre-focusing lever ensures that the objective does not come in contact
with the specimen and simplifies focusing.
After focusing on the specimen with the coarse adjustment knob, turn this
1 lever @ in the direction of the arrow and lock; the upper limit on coarse
adjustment movement is set at the locked position.
After changing a specimen, approximate refocusing is easily accomplis-
hed by rotating the coarse adjustment knob to reach the pre-focused
position. After this, all you have to do is make fine adjustments with the
fine adjustment knob.
Fig. 23
} Stage movement with the fine adjustment knob is not locked.
CAUTION When the pre-focusing lever is locked, a coarse movement stro-
ke is reduced due to the microscope’s mechanism, causing the
stage not to be able to be lowered to the lower limit. To lower
the stage to the lower limit, release the pre-focusing lever.
17
5-3 Stage
2 1 Placing the Specimen

1 } The dimensions of the slide glass should be 26 x 76 mm with thickness of
0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm.
} When observing very large specimens, remove the slide holder and place
the specimen directly on the stage.
Microscopy with Double-Slide Holder (Fig. 24)
1. Turn the coarse adjustment knob @ to lower the stage.
2. Open the spring-loaded curved finger 2 on the slide holder and place
one or two specimen slides on the stage from the front.
Fig. 24 3. After placing the sides as far as they will go, gently release the curved
finger.
Microscopy with Single-Slide Holder (Fig. 25)
The specimen side can easily be placed by sliding it into the slide holder
from the front.
Examining a whole specimen
Use one of the thin slide holders listed below that cause less interference
with the objectives*.
· U-HRD-4 · U-HLD-4 · U-HLS-4
* The applicable objectives are ones with 40X or less magnification (except
Apo series).
Fig. 25 Using an Oil Immersion Condenser
Use the optional U-SVRO (right-hand knob) or USVLO (left-hand knob)
slotted stage which prevents close contact between the specimen and
stage.
2 Using the Auxiliary Slide Holder

1
Applicable revolving nosepieces: U-D7RE, U-D7RES, U-P6RE,
U-D6BDRE, U-P5BDRE
} When a biological slide glass specimen is placed on the U-HLDT4/U-
HRDT4 two-slide holder (thick type), the slide holder may interfere with
the objective when the following conditions are combined;
· The objective in use has a short WD (Working Distance);
· The sspecimen being observed is the specimen placed on the rear
side of the side holder, and;
Fig. 26 · The stage is in a rotated position.
If the slide holder contacts the objective, set the provided auxiliary slide
holder @ as shown in the figure and observe only the specimen on the
front side of the slide holder.
18
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3 Adjusting the X- and Y-Axis Knob Tension (Fig. 27)
1. Hold the X-axis knob @ and slide up the Y-axis knob 2 up to expose
the adjustment knobs.
2 2. Turning the X-axis adjustment knob 3 or Y-axis adjustment knob |
clockwise (in the direction of the arrow) increases the tension and
4 counterclockwise decreases it.
3 CAUTION If the tension is adjusted to tight, a creaking sound may be heard
during stage travel, and the stage stopping accuracy may be
1
imperiled.
Fig. 27 } After long hours of use, the stage guide may be deviated and the stage
travel range may be decreased.
However, this is not malfunction and can be corrected easily as described
below.
[Treatment]
Horizontal direction: Hold the specimen holder and move the stage guide
to the left and right so that it hits the stoppers.
Vertical direction: Hold the upper stage and move it to the front and rear
so that it hits the stoppers.
Stage Feed Knob Rubber Caps (Optional)
} When the X- and Y-axis knobs are fitted with the rubber caps, the knobs
can be adjusted without slipping and fine adjustment is possible by
holding the knobs with a very light force. The knob rubber caps also
reduce fatigue after long hours of operation.
The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness
2 mm) knob rubbers are available.
To attach the knob rubbers:
First fit the larger knob rubber to the Y-axis (upper) knob from below it,
then fit the smaller knob rubber to the X-axis (lower) knob from below it.
19
4 Rotating the Stage (Fig. 28)
1
1. Slightly loosen the stage clamping screw @.
2. The stage can be rotated both clockwise and counterclockwise by the
stage clamping screw.
CAUTION A click may be heard and felt during rotation. However, this is
due to the construction of the substage and does not indicate
a malfunction.
} The angle of rotation varies depending on the positions of the X- and
Y-axis knobs.
Fig. 28
Angle of Rotation
Clockwise Counterclockwise
Right hand knobs 230° 20°
Left hand knobs 20° 230°
1 5 Adjusting the Stage Height (Figs. 29 & 30)
} By lowering the position of the substage, the microscope will accommo-

date specimens with maximum height of 35 mm. This is useful when
observing metallurgical specimens and other thick objects.
1. Lower the stage to the lower limit, then remove the stage from the
microscope.
2. Using the Allen screwdriver, loosen the substage bracket clamping screw
@ and remove the substage.
3. Turn the coarse adjustment knob and raise the focusing block 3 to where
the stopper screw 2 on the arm becomes visible.
Fig. 29
4. Using the Allen screwdriver, loosen and remove the upper stopper screw
2.
3 5. Reattach substage bracket and stage.
2 } Store the removed stopper screw 2 in a safe place so that it will not be
lost, if needed again.
Fig. 30
20
BX43
5-4 Observation Tube
1 Adjusting the Diopter

2
When diopter adjustment rings are provided on both the left and right, it
is possible to adjust the optimum vision for both the left and right eyes as
described below. The diopter adjustment rings may be provided either on
the eyepieces or the observation tube, and there is no problem even when
the rings are not provided on both of them.
The diopter adjustment ensures parfocality from low to high magnifications
1 and makes it possible to avoid defocusing even when the objective is
switched.
Fig. 31
1. Set the diopter adjustment rings on both sides to scale “0”.
2. Engage a high-power objective (40X or so) in the light path, look into
the right eyepiece with your right eye, and rotate the coarse and fine
adjustment knobs to bring the specimen into focus.
} Do not use an oil-immersion objective.
3. Engage a low-power objective (10X or so) in the light path, rotate the right
diopter adjustment ring @ to bring the specimen into focus. Do not touch
the coarse and fine adjustment knobs during the focusing.
4. Looking into the left eyepiece with your left eye, rotate the left diopter
adjustment ring 2 to bring the specimen in focus.
} The above procedure adjusts the diopter with reference to the right eye,
but it is also possible to adjust with reference to the left eye. In this case,
read the above procedure by inverting “right” and “left.”
} If an eyepiece equipped with a diopter adjustment ring is inserted into
the side equipped with another diopter adjustment ring of the observation
tube, perform the above procedure without moving the diopter adjustment
ring of the observation tube from the “0” position.
2 Using the Eye Shades (Fig. 32)
When Wearing Eyeglasses

Use with the eye shades in the normal, folded-down position. This will
prevent the eyeglasses from being scratched.
When Not Wearing Eyeglasses
Extend the folded eye shades in the direction of the arrow to prevent
extraneous light from entering between the eyepieces and eyes.
Fig. 32
21
3 Using the Eyepiece Micrometer Disk (Fig. 33)
1 When the WHN10X-H (WHN10X) eyepieces are used, an eyepiece
micrometer disk can be inserted in one of them. When the eyepiece
does not have a diopter adjustment mechanism, however, it is hard to
focus on the micrometer disk if the operator has poor eyesight. Should
that be the case, adjust the focus with eyeglasses on.
Use 24 mm dia. x 1.5 mm thick micrometer disks.
Following Fig. 33, turn the built-in micrometer-mounting frame 2 coun-
2 terclockwise to remove it from the eyepiece and place a micrometer
disk @.
Fig. 33
Screw the micrometer mounting frame back into the eyepiece sleeve.
} Be careful not to touch the lens surface with your finger.
} The micrometer-mounting frame may be tight for certain micrometer disks.
In this case, turn the frame slowly but with a stronger force than usual.
4 Selecting the Light Path of the Trinocular Tube

1
Slide the light path selector knob @ to select the desired light path.
Light path selector knob position

Trinocular tube
Pushed in Middle position Pulled out
U-TR30-2 20% for binocu-
lar eyepieces,
U-SWTR-3 100% for bino- 80% for camera
cular eyepieces 50% for binocu-
Fig. 34 U-TR30NIR* lar eyepieces, 100% for camera
50% for camera
50% for binocu-
100% for binocu-
U-TTR2 lar eyepieces,
lar eyepieces
50% for camera
* With the infrared trinocular tube, infrared observation up to 1000 nm is
possible. For details, consult your Olympus representative.
} The light path selector knob is located on the right side of the observation
tube, but it can be removed and attached on the left side when the U-TTR
is used.
After changing the knob position, be sure to tighten the knob firmly with
a coin.
22
BX43
1
5 Adjusting the Tilt (Figs. 35 to 38)
5° to 35°
With the U-TBI-3 (Fig. 35)
} Adjust the height and tilt of the observation tube to obtain the most
comfortable viewing position.
U-TBI-3 5° to 35°
U-TBI-3-CLT 5° to 35°
U-ETBI 0° to 25°
Fig. 35 U-TTBI 0° to 25°
U-TTR2 5° to 35°
U-TTLBI 0° to 27°
Holding the binocular section @ with both hands, raise or lower it to the
desired position.
CAUTION Never attempt to force the binocular section past the upper or
lower stop position. Applying excessive force could destroy the
limiting mechanism.
} The U-TBI-3/U-TBI-3-CLT can be used in combination with only one
intermediate attachment.
} If you need photography using the U-TBI-3/U-TBI-3-CLT, use the U-TRU
Fig. 36 trinocular tube.
With the U-ETBI/U-TTBI (Fig. 36)

The U-ETBI and U-TTBI are ergonomic observation tubes with normal
field, capable of the adjustments of the positioning angle (0° to 25°) as
well as front-rear positioning (by 45 mm) of the eyepieces. The U-ETBI is
the erect image model and the U-TTBI is the inverted image model, and
both models are of the same size.
CAUTION The intermediate attachments that can be combined with the
U-TTBI are limited. For details, please contact Olympus.
With the U-TTLBI (Fig. 37)

Fig. 37 The U-TTLBI is a Tilting, Telescopic, Lifting binocular tube with the following
adjustment capabilities.
· Eyepiece angle adjustment (0° to 27°)
· Eyepiece up-down position adjustment (45 mm)
2
· Observation tube front-rear position adjustment (55 mm)
} When adjusting the front-rear position of the observation tube, hold the
base of the observation tube as shown in Fig. 38.
} When storing the microscope, increase the angle of the eyepieces so as
to prevent the eyepieces from dropping.
} To adjust the up-down position of the eyepiece, turn the dial 2.
Fig. 38
23
6 Using Eyepieces Incorporating a Micrometer
2
} When the eyepieces in use incorporate a micrometer, the accuracy of
the left-right focusing adjustment (diopter adjustment) can be improved
further.
1. Looking into the right eyepiece with your right eye, turn the top of the
eyepiece @ so that the micrometer in the field of view looks sharpest
(Fig. 39).
1 2. Looking into the right eyepiece, rotate the coarse and fine adjustment
knobs to bring the specimen and micrometer (cross lines, etc.) in focus.
3. Looking into the left eyepiece with your left eye, rotate the diopter
Fig. 39
adjustment ring 2 to bring the specimen into focus.
5-5 Condenser
For how to adjust the centering of the condenser, see page 12.
1 Compatibility of Objectives and Condensers
Condenser
Objective Low Ultralow
Magnification Abbe Achromat/aplanat Swing-out
magnification magnification
U-AC2 U-AAC U-SC3
U-LC U-ULC-2
Applicable
1.25X
(FN 22)
2X Applicable (FN 26.5) Applicable
by moving top lens (FN 26.5)
Applicable out of the light path*
4X Applicable (FN 22) (FN 26.5)
(FN 22)**
10-60X Applicable Applicable Engage top lens in
100X (FN 26.5) (FN 26.5) light path (FN 26.5)
* When using the U-SC3 swing-out condenser together with a 1.25X to 4X objective, fully open the condenser aperture
iris diaphragm and use the field iris diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the
surroundings of the field of view may become dark.
} To obtain better illumination in camera recording using a 1.25X to 4X objective, use of the U-ULC-2 is recommended.
**When the U-LC is used with an objective with an NA of 1.0 or more, the NA of the lighting becomes insufficient.
} When the U-LC is used, it is required to attach the auxiliary lens (page 34).
24
BX43
5-6 Immersion Objectives

CAUTION Be sure to use the provided Olympus Immersion oil.
1 Using Immersion Objectives (Fig. 40)
1. Focus on the specimen with objectives in the order of lower-power to

higher-power ones.
2. Before engaging the immersion objective, place a drop of provided
immersion oil onto the specimen at the area to be observed.
3. Turn the revolving nosepiece to engage the immersion objective, then
focus using the fine adjustment knob.
CAUTION Since air bubbles in the oil will affect the image quality, make
sure that the oil is free of bubbles.
Fig. 40 a. To check for bubbles, remove the eyepiece and fully open the field and
aperture iris diaphragms, then look at the exit pupil of the objective inside
the observation tube. (The pupil should appear round and bright.)
b. To remove bubbles, turn the revolving nosepiece to repeatedly defocus
and refocus the oil immersion objective.
} If the condenser engraving shows a numerical aperture (NA) of 1.0 or
higher, the number applies only when oil is applied between the slide
glass and the top surface of the condenser. When oil is not present, the
NA is about 0.9.
4. After use, remove immersion oil from the objective front lens by wiping
with gauze slightly moistened with absolute alcohol.
CAUTION Caution in use of immersion oil

If immersion oil enters your eyes or contacts with your skin, imme-
diately take the following treatment.
Eyes: Rinse with fresh water (for 15 minutes or more).
Skin: Rinse with water and soap.
If the appearance of the eyes or skin is altered or pain persists,
immediately see your doctor.
5-7 Objectives with Correction Collar

} If the thickness scale setting of the objective with collection collar does
not match the cover glass thickness, the objective cannot manifest the full
performance. To prevent this, be sure to perform the following adjustment
when using an objective with collection collar.
Adjustment Procedure
1 · If the cover glass thickness is known, set the correction collar @ to that
scale value. (Fig. 41)
· If the cover glass thickness is unknown, adjust the correction collar @
and fine adjustment knob alternately until the positioning with the highest
contrast is obtained.
Fig. 41
} Be careful not to touch the correction collar @ when turning the revolving
nosepiece.
25
6 CAMERA RECORDING
} Use a trinocular observation tube such as the U-TR30-2 or U-SWTR-2 when shooting the video or digital camera picture
of the microscope images.
The trinocular tube accepts a camera adapter (certain TV adapters necessitate a camera mount adapter).
} Be sure to adjust the parfocality before using a camera adapter. Otherwise, the focusing of the camera image will not
match that of the image observed through eyepieces. For the parfocality adjustment method, refer to the instruction
manual for the camera adapter in use.
For details, refer to the instruction manuals for these accessories.
1 System Chart
TV camera/digital camera
Camera mount adapter
C-mount camera adapter
Camera adapter
Trinocular observation tube

U-TR30-2
U-SWTR-3
U-TTR2, etc.
2 Selecting the Camera Adapter Magnification

The image pickup area is determined by the size of the image sensor used in the TV camera or digital camera and the
magnification of the camera adapter.
The following illustrations show the image pickup areas of camera adapters when the SWH10X-H eyepieces with a FN
of 26.5 are used (the FN of the WHN10X eyepieces is 22).
1-inch image sensor
2/3-inch image sensor
1/2-inch image sensor
WHN10X: FN22
SWH10X-H: FN 26.5
When a 0.5X camera adapter is used When a 1X camera adapter is used
26
BX43
7 TROUBLESHOOTING GUIDE
Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,
please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire
list, please contact your local Olympus representative for assistance.
Problem Lamp Cause Remedy Page

Bulb does not light. LED Power is not ON. Set the main switch to “ I ” (ON). 10
Common Power cord is unplugged. Plug power cord into the power
37
outlet.
Common Connectors are connected Connect connectors securely.
37
improperly.
Halogen Bulb is not attached. Attach a halogen bulb. 34
Halogen Bulb is burned out. Replace bulb. 34
Halogen The TL4 is not ON. Set the main switch of the TL4 to
37
“ I ” (ON).
Bulb is blown immediately. Halogen The bulb is not a designated Replace bulb with a designated
1
one. one.
Field of view is dark. LED The LIM setting is inappropriate. Set LIM again. 13
Common Aperture and field iris diaphragms Adjust them to proper sizes.
12
are not opened wide enough.
Common Light path selector knob is set to Set the knob to the or
22
the position. position.
Common Condenser is not properly Center the condenser.
12
centered.
Common Condenser height is too low. Adjust correctly. 24
Halogen ND filter is engaged. Disengage the ND filters. 15
Brightness cannot be varied. LED LIM is not ON. Switch the LIM function OFF. 13
Halogen LED brightness adjustment knob Use the brightness adjustment
37
is rotated. knob of the TL4.
Condenser is not properly Center the condenser.
Common 12
centered.
Common Condenser height is too low. Adjust correctly. 24
Field of view is obscured or not Common Light path selector knob is set in Set to the desired click position.
22
evenly illuminated. an intermediate position.
Common O b j e c t i v e i s n o t c o r r e c t l y Make sure that the revolving
engaged/ nosepiece clicks properly into 12
place.
Common Revolving nosepiece is not Push the slide dovetail all the way
35
attached properly. until it is stopped.
Condenser is not attached Re-attach it .
Common 36
properly.
Common An objective that falls outside Use a condenser to match the
of the condenser’s illumination purpose. 24
range is used.
Condenser is not properly Center the condenser.
Common 12
centered.
Common Field iris diaphragm is stopped Open the field iris diaphragm until
12
down too far. it circumscribes the field.
Halogen Bulb is not mounted correctly. Push the pins of halogen bulb all
34
the way until the stop position.
27
Dirt or dust is visible in the field Common Dirt/dust on the eyepieces. Clean thoroughly.
of view.
Common Dirt or dust on condenser top
3
lens.
Common Dirt/dust on the specimen.
Visibility is poor Common A non-UIS/UIS2 objective is Use only UIS2/UIS series objecti-
32
· Image is not poor. used. ves with this microscope.
· Contrast is poor. Common Condenser height is too low. Adjust correctly. 24
· Details are indistinct.
Common Aperture iris diaphragm is not Open aperture iris diaphragm.
· Image glares. 12
opened enough.
Common Revolving nosepiece is not at- Push the slide dovetail all the way
35
tached properly. until it is stopped.
Common Correction collar on correction While focusing, turn the correction
collar-equipped objective is not collar to find the best position. 25
properly adjusted.
Common Front lens of objective is dirty. Clean objective. 3
Common Immersion oil is not being used Use immersion oil.
25
with an oil immersion objective.
Common Immersion oil contains bubbles. Remove the bubbles. 25
Common Recommended immersion oil is Use an Olympus-designated
25
not used. immersion oil.
Common Dirt/dust on specimen. Clean it.
3
Common Dirt/dust on condenser top lens.
One side of image is blurred. Common Objective is not correctly engaged Make sure that revolving nosepiece
12
in light path. clicks into place correctly.
Common Stage is not correctly mounted. Re-attach it. 18
Common Revolving nosepiece is not at- Push the slide dovetail all the way
35
tached properly. until it is stopped.
Common Specimen is mounted upside Mount specimen correctly.
18
down.
Image appears to waver. Common Revolving nosepiece is not Push the slide dovetail all the way
35
attached properly. until it is stopped.
Common Objective is not correctly engaged Make sure that revolving nosepie-
12
in light path. ce clicks into place correctly.
Common Condenser is not properly Center the condenser.
12
centered.
Coarse adjustment knob is hard Common Tension adjustment ring is Loose ring.
16
to turn. tightened excessively.
Common You are trying to raise stage Unlock pre-focusing lever.
without coarse adjustment knob
17
while pre-focusing lever is kept
locked.
Image cannot be focused: Common Tension adjustment ring is too Tighten ring.
· Objective drifts down by itself. loose. 16
· Fine adjustment knob slips.
28
BX43

Coarse adjustment will not go all Common Pre-focusing lever is locked at a Unlock pre-focusing lever.
17
the way up. low position.
Coarse adjustment will not go all Common Condenser holder is too low. Raise condenser holder.
36
the way down.
Objective makes contact with Common Specimen is mounted upside Mount specimen correctly.
specimen before focus is down. 18
obtained.
Field of view of one eye does not Common Interpupillary distance is incorrect. Adjust interpupillary distance. 11
match that of the other.
Common Diopter adjustment is incorrect. Adjust diopter. 21
Common Different eyepieces are used on Change one eyepiece to match
left and right. the other so that both sides are 32
the same type.
Common Your view is not accustomed to Upon looking into eyepieces,
microscope observation. try looking at overall field before
concentrating on specimen ran-
ge. You may also find it helpful 21
to look up and into distance for
a moment before looking back
into microscope.
Image shifts when you touch Common Stage is not properly mounted. Clamp stage.
18
stage.
X- and Y-axis knobs are too tight Common Tension of X- and Y-axis knobs is Adjust tension.
19
or too loose. too high or too low.
Stroke has reduced. Common Stage guide is deviated. Correct deviation as described. 19
29
8 SPECIFICATIONS
Item Specification
1. Optical system UIS2 (UIS) optical system (featuring infinity correction)
2. Illumination Built-in transmitted Koehler illumination
FN (Field Number): 26.5 (super-widefield compatible)
LED illuminator (continuously variable): U-LHLEDC
Power consumption: 0.5 A
Optional 6V 30W halogen bulb (continuously variable)
Power supply unit TL4: 100-120/220-240 V $ , 0.85/0.45 A, 50/60 Hz.
Power consumption: 45 W
3. Focusing mechanism Stage up-down movement by roller guide (rack & pinion)
Stroke per rotation: 0.1 mm (fine), 17.8 mm (coarse)
Full stroke range: 25 mm
Upper coarse adjustment limit stopper
Tension adjustment on coarse focus adjustment knob.
4. Revolving nosepiece Type BX43-5RES U-D6RE
5-position revolving nosepiece with
Universal 6-position revolving nosepiece
readout function
Attachable Transmitted light DIC slider
module Transmitted light analyzer
5. Observation tube Type U-BI30-2 U-TBI-3/U-TBI3-CLI U-TR30-2 U-SWTR-3
Widefield, tilting Widefield, Super- widefield,
Widefield binocular
binocular trinocular trinocular
Field No. 22 26.5
Tube 5-35°
30° 30°
inclination (continuously variable)
Interpupillary
distance 50-76 mm
adjustment
Light path 3 steps: @ Binocular 100%
selection Not available 2 Bi 100%, Cam 80%
3 Camera 100%
6. BX stage Type U-SVRB-4 U-SVLB-4
Coaxial knobs on bottom right. Coaxial knobs on bottom left.
Rectangular ceramic-coated, wire-driven stage
Size 156 mm (D) x 191 mm (W)
Movement X- and Y-axis knob with adjustable tension. Movement range: 52 mm in vertical (Y)
mechanism direction, 76 mm in horizontal (X) direction.
Slide holder
U-HLST-4 (Thick type)
(single-slide
U-HLS-4 (Thin type)
holder)
Slide holder
U-HLDT-4 (Left-opening, thick type) U-HRDT-4 (Right-opening, thick type)
(double-slide
U-HLD-4 (Left-opening, thin type) U-HRD-4(Right-opening, thin type)
holder)
30
BX43
Item Specification
7. Condenser Type U-LC U-AC2 U-SC3 U-AAC
Low magnification Abbe Swing-out Achromat/ Aplanat
Max. N.A . 0.75 1.10 0.9 1.40
Applicable 2X - 60X 4X 1.25X 10X - 100X
objective (Widefield: FN 22) (Widefield: FN 22) (Widefield: FN 22) (Super widefield:
powers 10X - 100X 2X - 100X FN 26.5)
(Super widefield: (Super widefield:
FN 26.5) FN 26.5)
8. Operating · Indoor use.
environment · Altitude: Max. 2000 meters
· Ambient temperature: 5° to 40°C (41° to 104°F)
· Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly through
70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F).
· Supply voltage fluctuations: ±10%
· Pollution degree: 2 (in accordance with IEC60664-1)
· Installation/Overvoltage category: II (in accordance with IEC60664-1)
9. Rating Input rating: 5 V, 2.5 A
AC adapter SA115B-05V: 100V-240Vac 50/60 Hz, 0.4 A
31
9 OPTICAL CHARACTERISTICS (UIS2 Series)
UIS series objectives not listed here can also be combined with this microscope.
The following table shows the optical characteristics of com-

Magnification Objective type
binations of eyepieces and objectives. The figure on the right
shows the performance data engraved on the objectives. Number of aperture
Mechanical tube
length Oil: Oil immersion
NOTE Iris: Iris diaphragm
Refer to the latest catalogue or consult your local Olympus Field number (FN)
representative for the updated information on the eyepieces Color band Cover glass thickness
and objectives that can be combined with this microscope.
Optical character Eyepiece

Cover
glass Reso- WHN10X (FN22)
Magnifi- W.D.
cation N .A. (mm) thick- lution Remark
ness (µm) Depth of
Objective Total mag. focus Field of
Series Marking (mm) view
(µm)
PLN (PH) PlanN (Ph) 2X 0.06 5.8 – 5.59 20X 560.1 11.0
Plan 4X 0.1 18.5 – 3.36 40X 175.0 5.5
Achromat 10X (Ph) 0.25 10.6 – 1.34 100X 28.0 2.2
(FN 22) 20X (Ph) 0.4 1.2 0.17 0.84 200X 9.27 1.1
40X (Ph) 0.65 0.6 0.17 0.52 400X 3.04 0.55
PH: Phase 50XOI 0.5-0.9 0.2 0.17 0.37 500X 1.7 0.44 Oil immersion/Iris
contrast 100XO (Ph) 1.25 0.15 – 0.27 1000X 0.69 0.22 Oil immersion
UPLFLN (PH) UPlanFLN 4X (Ph) 0.13 17.0 – 2.58 40X 127.2 5.5
Plan Semi (Ph) 10X2 (Ph) 0.3 10.0 – 1.12 100X 22.4 2.2
Apochromat 20X (Ph) 0.5 2.1 0.17 0.67 200X 7.0 1.1
(FN 26.5) 40X (Ph) 0.75 0.51 0.17 0.45 400X 2.52 0.55
40XO 1.3 0.2 0.17 0.26 400X 1.27 0.55 Oil immersion
60X 0.9 0.2 0.17 0.37 600X 1.5 0.37 Correction collar
60XOI (Ph) 0.65-1.25 0.12 0.17 0.27 600X 0.98 0.37 Oil immersion/Iris
PH: Phase 100XO2 (Ph) 1.30 0.2 0.17 0.26 1000X 0.66 0.22 Oil immersion
contrast 100XOI2 0.6-1.30 0.2 0.17 0.26 1000X 0.66 0.22 Oil immersion/Iris
UPLSAPO UPlanSApo 4X 0.16 13.0 – 2.10 40X 99.6 5.5
Plan 10X2 0.4 3.1 0.17 0.84 100X 15.9 2.2
Apochromat 20X 0.75 0.6 0.17 0.45 200X 4.29 1.1
(FN 26.5) 20XO 0.85 0.17 – 0.39 200X 3.5 1.1 Oil immersion
40X2 0.95 0.18 0.17 0.35 400X 1.9 0.55 Correction collar
60XW 1.2 0.28 0.17 0.28 600X 1.03 0.37 Water immersion
PLAPON PlanApoN
1.25X 0.04 5.0 – 8.39 12.5X 1326.8 17.6
Plan
2X 0.08 6.2 – 4.19 20X 398.3 11.0
Apochromat
(FN 26.5)
UApoN UApoN 20XW340 0.7 0.35 0.17 0.48 200X 4.08 1.1 Water immersion
Apochromat Water immersion/
40XW340 1.15 0.25 0.13-0.25 0.29 400X 1.29 0.55
(FN 22) Correction collar
40XO340 1.35 0.1 0.17 0.25 400X 1.2 0.55 Oil immersion
32
BX43
10 ASSEMBLY
10-1 Assembly Diagram

The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of
assembly.
Assembly steps enclosed in will be detailed on the subsequent pages.
The module numbers shown in the following diagram are merely the typical examples. For the modules with which the
module numbers are not given, please consult your Olympus representative or the catalogues.
CAUTION When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.
} Most assembly operations are possible by using the Allen screwdriver ( ) provided with the microscope.
However, the assembly of the reflected light illuminator requires the use of the provided Allen wrench ( ) for clamping
the internal screws (to ensure the performance, we recommend that you have your Olympus representative assemble
or remove this module).
Eyepieces 7 Observation tube

WHN10X (FN 22) U-BI30-2
WHN10X-H (FN 22) U-TTLBI Intermediate attachment
SWH10X-H (FN 26.5) U-TR30-2, etc. U-EPA2
MICRO SWH10X (FN 26.5), etc. U-DO3
U-TRU, etc.
Observation tube
clamping screw Reflected light
6 mercury/xenon
Revolving nosepiece lamp housing
BX43-5RES
U-5RE-2
9
U-D6RE Vertical illuminator
U-D7RES, etc. Arm BX3-URA
BX3-ARM BX3-RFAS, BX3-RFAA, etc.
8 Revolving nosepiece
UIS2 series clamping screw Halogen bulb
objective 6W30WHAL
Slide holder 4
Slide holder clamping knobs 1
U-HLDT-4
U-HRDT-4, etc. Lamp housing adapter
U-LS30ADP
5
Stage clamping 4
knob 4
Stage Lamp socket
U-SVRB-4 U-LS30-5
U-SVLB-4, etc.
Lamp cable
3 LED lamp housing
Microscope frame U-LHLEDC
BX43F
2
Condenser Auxiliary lens for
4
clamping knob U-LC
10
Stage feed knob
rubber caps
U-SHG 11
U-SHGT
AC adapter for Power cord

Condenser microscope Power supply unit
U-LC TL4
U-AC2
U-SC3
U-UCD8 (T2), etc.
12 12
Power cord
33
10-2 Detailed Assembly Procedures
2 Attaching
Condenser
the Auxiliary Lens for the U-LC (Fig. 42)
} It is not necessary to attach the auxiliary lens if the condenser in use is

other than the U-LC.
1 1. Attach the auxiliary lens @ provided with the condenser into the tip of the
LED lamp housing C (U-LHLEDC) 2 or lamp housing adapter (U-LS30ADP)
by screwing the lens in the direction of the arrow.
2 CAUTION Be careful not to touch the lens with your finger.
Fig. 42
5 3 3 Attaching the LED Lamp Housing C (Fig. 43)
4 1. Fit the LED lamp housing @ into the mount hole on the rear of the
microscope by aligning the clamping screw 2 and screw hole 3.
2. Using the Allen screwdriver, tighten the clamping screw 2.
2 3. Insert the connector | of the lamp housing into the connector 5 on the
microscope.
1
Fig. 43
4 Attaching the Halogen Lamp Socket (Fig. 44)
Installing the U-LS30ADP LS30 Adapter (Fig. 44)

1. Peel off the backing of double-side adhesive tape on the cable holder
1 @ and attach the holder on the specified position.
The area (enclosed with broken lines) on the outer side is for use in
attaching the cable holder for another option.
2. Attach the adapter in the same way as steps 1 and 2 in the LED lamp
housing C attaching procedure described above.
Fig. 44
34
BX43
Installing the Halogen Bulb (Fig. 45)
Use only the designated bulb 6V30WHAL (PHILIPS 5761).
2 1. Holding the bulb 2 a piece of gauze, insert the bulb pins 3 straight and
fully into the pin holes | on the lamp socket.
4 CAUTION To prevent reduced bulb life or cracking, do not touch the bulb
3 with bare hands. If fingerprints are accidentally left on the bulb,
wipe the bulb with a soft cloth.
Fig. 45
2. Insert the lamp cable 5 in the direction shown in the figure 46.
3. Aligning the guide pins 6 with the guide pin holes † at the rear of the
microscope frame, gently push the lamp socket all the way into place.
CAUTION Caution for Bulb Replacement During Use or Right After Use
7
The bulb and the lamp socket are areas near these will be
5
extremely hot during and right after use.
Fig. 46 Set the main switch to “ ” (OFF), disconnect the power cord
from the wall outlet, then allow the old bulb and lamp socket
to cool before replacing the bulb with a new of the designated
type.
4 Pass the lamp cable through the cable holder @ (Fig. 44).
8 Connecting the Lamp Cable (Fig. 47)
9 Connect the cable connector 8 to the connector 9 of the TL4 power

supply unit.
Fig. 47
8 Attaching the Revolving Nosepiece (Fig. 48)

1. Rotate the coarse adjustment knob to lower the stage.
2. Loosen the revolving nosepiece clamping screw @ slightly. Do not loosen
2 3
it too much because doing so makes it impossible to attach the revolving
nosepiece.
3. Hold the revolving nosepiece so that the mount dovetail 2 is horizontal
1
and insert it all the way into the dovetail groove of the microscope arm
3.
4. Tighten the revolving nosepiece clamping screw @.
} When attaching or detaching a revolving nosepiece with a cable, be sure
Fig. 48 to disconnect the cable from the revolving nosepiece in advance.
35
Use only coded 5 position nosepiece for BX43 (BX43-5RES)
} Distribute its cable | toward the rear panel by passing it below the arm,
4 and connect the cable to the connector no the rear panel.
} As shown in Fig. 49, connect to the connector by letting the cable into
the cable holder (attached with BX43-5RES) attached to the rear panel.
Fig. 49
2 9 Attaching the Condenser (Fig. 50)

1. Turn the coarse adjustment knob @ to raise the stage to its upper limit.
2. Turn the condenser height adjustment knob 2 to lower the condenser
holder to its lowest position.
3. Fully loosen the condenser clamping knob 3.
4. Position the condenser so that the aperture scale is located in front.
Gently slide the condenser along the dovetail all the way until it is
stopped.
1
3 } When attaching the U-SC3 swing-out condenser, align the positioning pin
at the back of the condenser with the groove in the condenser sleeve.
Fig. 50
CAUTION · When attaching a swing-out condenser with a positioning pin at
the back such as the U-SC3, attach by aligning the positioning
pin with the groove in the condenser sleeve.
· When attaching a condenser with swing-out top lens, swing out
the top lens out of the way before inserting the condenser.
5. Tighten the condenser clamping knob and gently raise the condenser
holder to its highest position.
10 Attaching the Eyepieces (Fig. 51)
Gently insert the eyepieces all the way into the eyepiece sleeves.
CAUTION · When using the U-BI30-2 binocular tube, eyepieces with a po-
sitioning pin cannot be attached because the U-BI30-2 does
1 not have the positioning notch.
· When using an eyepiece with micrometer, attach it to the right-
2 hand eyepiece sleeve.
When doing so, make sure that the eyepiece positioning pin @
fits into the notch 2 on the eyepiece sleeve.
Fig. 51 · The super-widefield trinocular observation tube is equipped with
a positioning notch on both the left and right eyepiece sleeves.
Make sure that the positioning pins on both eyepieces fits into
the respective notches.
36
BX43
11 Attaching the AC Adapter

CAUTION · Cables and cords are vulnerable when bent or twisted. Never
subject them to excessive force.
2 · Make sure that the main switch is set to “ ” (OFF) before
connecting the power cord (Fig. 52).
1 1. Insert the connector @ of the AC adapter firmly into the connector 2 on
the microscope.
CAUTION Always use the designated AC adapter. Using a non-designated
AC adapter may cause malfunction.
Fig. 52
12 Attaching the Power Cord

CAUTION Always use the power cord provided by Olympus. If no power
2 cord is provided with the microscope, please select the proper
power cord by referring to section “PROPER SELECTION OF THE
POWER SUPPLY CORD” at the end of this instruction manual.
Operation Using the AC Adapter (Fig. 53)
1 1. Insert the connector @ of the power cord firmly into the input connector
2 of the AC adapter (Fig. 53).
Fig. 53
CAUTION The power cord should be connected to a grounded/earthed,
3-conductor power outlet. If the power outlet is not grounded/
earthed properly, Olympus can no longer warrant the electrical
safety performance of the equipment.
2. Plug the power cord plug 3 into the wall outlet | (Fig. 55).
CAUTION Should the power cord come in contact with the hot lamp socket,
the power cord could melt and cause electric shock. Be sure
to route the power cord away from the lamp socket.
Operation Using the TL4 Power Supply Unit (Fig. 54)

2 1 } The following operation is not required when the LED lamp is used.
Fig. 54 1. Insert the connector @ of the power cord firmly into the input connector
2 of the TL4 (Fig. 54).
2. Plug the power cord plug 3 into the wall outlet | (Fig. 55).
3 4
Fig. 55
37
11 HALOGEN LAMP SOCKET INSPECTION SHEET
{ For safe use of the lamp socket, we recommend performing the following inspection periodically (every time you replace
the lamp bulb and at least every 6 months).
{ The table below identified the check items to be observed. Put (X) if not applicable or ( ) if applicable.
{ If there is any ( ) mark noted, immediately stop use of the product, and consult Olympus for detailed inspections or replace
the lamp socket.
{ If you detect an abnormality other than that listed below or with other Olympus product, also stop the use of the product and
contact Olympus for detailed inspections.
{Note that the service, replacement and detailed inspections are charged after expiration of the warranty period.
If you have any questions, please contact Olympus.
Check results (Date)

Check items / / / /
1. More than 8 years have passed since original purchase or the total power
ON time exceeds 20,000 hours.
2. Lamp does not light sometimes even though the main switch is set to on.
3. Illumination flickers when you move the lamp cable or lamp socket.
4. Scorching or burning odor is produced during use.
5. Illumination still flickers after replacement with a new lamp bulb.
6. Deformation, backlash, or looseness, etc. when you assemble the lamp socket.
7. Extreme discoloration of the lamp socket connection terminal or lamp socket
lamp bulb mount.
8. Discoloration, deformation or cracking of the lamp socket.
9. Melting, crack, deformation or solidification of the lamp cable or a wiring part.
10. Increased frequency of servicing compared to similar devices put into use at
the same time as the lamp socket.
* When the Check Result columns become insufficient, copy this sheet.
38
BX43
PROPER SELECTION OF THE POWER SUPPLY CORD

If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and
“ Certified Cord ” below:
CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the
electrical safety of the equipment.
Specifications
Voltage Rating 125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
Current Rating 6A minimum
Temperature Rating 60˚C minimum
Length 3.05 m maximum
Fittings Configuration Grounding type attachment plug cap. Opposite terminates in molded-on IEC
configuration appliance coupling.
Table 1 Certified Cord

A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an
agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in
Table 1. In case you are unable to buy locally in your country the power supply cord which is approved by one of the
agencies mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in
your country.
Certification Certification
Country Agency Mark Country Agency Mark
Argentina IRAM Italy IMQ
JET, JQA, TÜV,

Australia SAA Japan
UL-APEX/ MITI
Austria ÖVE Netherlands KEMA
Belgium CEBEC Norway NEMKO
Canada CSA Spain AEE
Denmark DEMKO Sweden SEMKO
Finland FEI Switzerland SEV
United ASTA
France UTE
Kingdom BSI
Germany VDE U.S.A. UL
Ireland NSAI
39
Table 2 HAR Flexible Cord
APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS
Alternative Marking Utilizing

Printed or Embossed Harmoni- Black-Red-Yellow Thread (Length
zation Marking (May be located of color section in mm)
Approval Organization
on jacket or insulation of internal
wiring) Black Red Yellow
Comite Electrotechnique Belge

CEBEC <HAR> 10 30 10
(CEBEC)
Verband Deutscher Elektrotechniker

<VDE> <HAR> 30 10 10
(VDE) e.V. Prüstelle
Union Technique de I´Electricite´

USE <HAR> 30 10 30
(UTE)
Instituto Italiano del Marchio di

IEMMEQU <HAR> 10 30 50
Qualita´ (IMQ)
British Approvals Service for Electric

BASEC <HAR> 10 10 30
Cables (BASEC)
N.V. KEMA KEMA-KEUR <HAR> 10 30 30
SEMKO AB Svenska Elektriska

SEMKO <HAR> 10 10 50
Materielkontrollanstalter
Österreichischer Verband für

<ÖVE> <HAR> 30 10 50
Elektrotechnik (ÖVE)
Danmarks Elektriske Materialkontroll

<DEMKO> <HAR> 30 10 30
(DEMKO)
National Standards Authority of Ireland

<NSAI> <HAR> 30 30 50
(NSAI)
Norges Elektriske Materiellkontroll

NEMKO <HAR> 10 10 70
(NEMKO)
Asociacion Electrotecnica Y
<UNED> <HAR> 30 10 70
Electronica Espanola (AEE)
Hellenic Organization for

ELOT <HAR> 30 30 70
Standardization (ELOT)
Instituto Portages da Qualidade

np <HAR> 10 10 90
(IPQ)
Schweizerischer Elektro
SEV <HAR> 10 30 90
Technischer Verein (SEV)
Elektriska Inspektoratet SETI <HAR> 10 30 90
Underwriters Laboratories Inc. (UL) SV, SVT, SJ or SJT, 3 X 18AWG

Canadian Standards Association (CSA) SV, SVT, SJ or SJT, 3 X 18AWG
40
MEMO

MEMO

Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan
Wendenstrasse 14-18, 20097 Hamburg, Germany
3500 Corporate Parkway, Center Valley, Pennsylvania 18034-0610, U.S.A.
491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373
31 Gilby Road, Mount Waverley, VIC., 3149, Melbourne, Australia
5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.
Printed in Japan on April 00, 2010 M 010-01


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INSTRUCTIONS

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3 FLOW OF OBSERVATION 8,9

4 SIMPLIFIED OBSERVATION PROCEDURE 10-12

4-1 Basic Operation (Until Observation of Specimen)...................................................................... 10,11

5 USING THE CONTROLS 13-25

5-2 Focusing Block................................................................................................................................................................................ 16,17

5-4 Observation Tube................................................................................................................................................................... 21-24

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5-7 Objectives with Correction Collar............................................................................................................................... 25

1 System Chart.......................................................... 26 2 Selecting the Camera Adapter

7 TROUBLESHOOTING GUIDE 27-29

9 OPTICAL CHARACTERISTICS (UIS2 Series) 32

11 HALOGEN LAMP SOCKET INSPECTION SHEET 38

PROPER SELECTION OF THE POWER SUPPLY CORD........................................................... 39,40

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SAFETY PRECAUTIONS (Fig. 1)

1. After the equipment has been used in an observation of a specimen that

Operation Using the LED Lamp

Operation Using the Halogen Bulb

Designated bulb 6V30WHAL (PHILIPS 5761)

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Caution engraving Halogen lamp socket

Caution label Rear panel

2 Maintenance and Storage

Interpupillary distance adjustment

Condenser height adjustment

Diopter adjustment ring

Slide holder clamping lever

Aperture iris diaphragm ring

Condenser centering screws

Field iris diaphragm ring

Y-axis knob (Page 20)

X-axis knob (Page 20)

Coarse adjustment tension

LIM setting switch

LED brightness adjustment

LIM indicator LIM ON-OFF switch

Low Magnification Condenser Swing-out Condenser

Aperture iris diaphragm

<<Modules for halogen lamp operation>>

Guide pin holes

Power Supply Unit

Lamp socket connector

(Controls Used) (Page)

LED lamp Halogen bulb

Place the specimen on the stage. 6Slide holder (P. 19)

Engage the 10X objective in the light path. 8Revolving nosepiece

Bring the specimen in focus. 9Coarse/fine adjustment knobs (P. 17)

aBinocular tube (P. 21)

Engage the desired objective in the light path

Insert the required filters. gFilter mount (P. 14)

Adjust the brightness. 2LED brightness |Brightness (P. 13)

1 Press the main switch of the microscope frame to “ I ” (ON).

4 3 Rotate the knob to lower the stage. (Details: Page 18)

5 Rotate the revolving nosepiece to engage the 10X objective in the

Now you can observe the magnified image of the specimen. To

4-2 Microscope Adjustments (How to Improve the Observed Image)