VISVESVARAYA TECHNOLOGICAL UNIVERSITY

“JNANA SANGAMA”, BELAGAVI-590018

Project Report
on
“Antimicrobial studies of TiO2 nanoparticles and Bioactive
components from Periplaneta americana”
Project is submitted in partial fulfillment of the requirements for the award of the degree
BACHELOR OF ENGINEERING
IN
BIOTECHNOLOGY

Submitted By:
Bhagysree Hosamani 1AY19BT004
Divyashree R 1AY19BT008
Girish 1AY19BT009
Sagar Vasudev Kesari 1AY19BT019

Place of investigation:
ACHARYA INSTITUTE OF TECHNOLOGY
Soldevanahalli, Bangalore-5600107

Under the Guidance of:

Mrs. Liny P,
Assistant Professor,
Dept. of Biotechnology,
AIT, Bangalore.

DEPARTMENT OF BIOTECHNOLOGY
ACHARYA INSTITUTE OF TECHNOLOGY
(Affiliated to Visvesvaraya Technological University, Belagavi, Recognized by AICTE and Accredited by NBA, New Delhi)
Acharya Dr.Sarvepalli Radhakrishnan Road,
Soldevanahalli, Bangalore-5600107.
(2022-23)
 DEPARTMENT OF BIOTECHNOLOGY
ACHARYA INSTITUTE OF TECHNOLOGY
(Affiliated to Visvesvaraya Technological University, Belagavi, Recognized by AICTE, New Delhi)
Acharya Dr. Sarvepalli Radhakrishnan Road, Soldevanahalli, Bengaluru-560107
2022-2023

Certificate
This is to certify that the project work entitled “Antimicrobial studies of TiO2 nanoparticles
and Bioactive components from Periplaneta americana” carried out by BHAGYSHREE
HOSAMANI(1AY19BT004), DIVYASHREE R (1AY19BT008), GIRISH(1AY19BT009), and
SAGAR VASUDEV KESARI(1AY19BT019), are bonafide students of Acharya Institute of
Technology, Bengaluru in partial fulfillment for the award of the degree of Bachelor of Engineering
in Biotechnology of the Visvesvaraya Technological University, Belagavi during the year 2022-23. It
is certified that all corrections/suggestions indicated for Internal Assessment have been incorporated in
the report deposited in the departmental library. The project work report has been approved as it satisfies
the academic requirements in respect of project work prescribed for the said Degree.

…………………… …………………… ……………………

Guide HOD Principal
Mrs. Liny P, Dr. Suneetha T B, Dr. Rajath Hegde M M,
Assistant Professor, HOD, Principal,
Department of Biotechnology, Department of Biotechnology, Acharya Institute of Technology,
AIT, Bangalore. AIT, Bangalore. Bangalore.

External Viva:
Name of the Examiners Signature with date

1.
2.
 DECLARATION

We BHAGYSHREE HOSAMANI, DIVYASHREE R, GIRISH and SAGAR VASUDEV

KESARI students of fourth year, B.E., Biotechnology, “ACHARYA INSTITUTE TECHNOLOGY”
declare that the project work titled “Antimicrobial studies of TiO2 nanoparticles and Bioactive

components from Periplaneta americana” has been carried out and submitted in partial fulfill of the
course requirements for the award of degree in Bachelor of Engineering of Visvesvaraya Technological
University, Belagavi, during the academic year 2022-23.

Place: Bangalore
Date:

Signatures of students:

Ms. BHAGYSHREE HOSAMANI (1AY19BT004)

Ms. DIVYASHREE R (1AY19BT008)

Mr. GIRISH (1AY19BT009)

Mr. SAGAR VASUDEV KESARI (1AY19BT019)

 AKNOWLEDGEMENT

We express our deep sense of gratitude to our Institution, “ACHARYA INSTITUTE OF

TECHNOLOGY”, Bangalore, which has provided us an opportunity to fulfill our long-cherished dreams.

We thank our beloved Managing Director Mr. KRISHNA BASANI REDDY who provided us the
much- needed support and encouragement all through in making this work a success.

We are highly obliged to record our gratitude to our Principal, Dr. RAJATH HEGDE M M, Acharya
Institute of Technology, Bangalore, for his encouragement and support for the completion of our project work.

We consider this as a great privilege to express our sincere thanks to our beloved HOD Dr.
SUNEETHA T B, Dept. of Biotechnology, Acharya Institute of Technology, Bangalore for her belief in our
abilities to carry out this project work. She has been a constant source of encouragement and constructive
suggestions helped us immensely throughout our project work.

With a deep sense of gratitude, we express our heartiest thanks to our beloved guide, Mrs. LINY P,
Assistant professor, Department of Biotechnology, AIT, Bangalore for suggesting this valuable topic, her
effective guidance and support to carry out this project work along with her keen observation, gentle guidance,
constant encouragement and highly supportive nature.

We take this opportunity to express our sincere thanks to all the Biotechnology teaching and
nonteaching staff for their direct and indirect help all the way.

We thank our beloved parents, family and our friends who provided us the much needed psychological
impetus and encouragement all through in making this work a success.

BY:
Bhagyshree Hosamani
Divyashree R
Girish
Sagar Vasudev Kesari

i
 ABSTRACT
Multidrug resistant bacterial strains have emerged as a result of the widespread use
of antibiotics, food safety and human health are currently at risk. Metal oxide nanoparticles
have drawn the most attention as potential novel antibacterial agents. Due to its
photocatalytic properties, chemical stability, non-toxicity, low cost, and widespread
acceptance as a safe substance, titanium dioxide (TiO2) has been specifically considered as
an appealing antimicrobial compound. It also has excellent antifungal and antimicrobial
properties against a wide range of both gramme positive and gram-negative bacteria.
Cockroach extracts have in vitro antibacterial action against methicillin-resistant
Staphylococcus aureus (MRSA). Extracts with solvent like chloroform and ethanol were
made, and they were tested against Staphylococcus aureus and E coli. Ethanol extracts from
cockroaches guts and brains shown better activity against methicillin-resistant
Staphylococcus aureus and Escherichia coli with a mean diameter of zone of inhibition.

Key words: Periplaneta americana; nanocomposites; antibacterial ; anti-biofilm.

ii
 TABLE OF CONTENTS
Acknowledgement ............................................................................................ (i)
Abstract............................................................................................................(ii)

Sl no Content Page no
1 Introduction 1-2
2 Review of Literature 3-4
3 Objectives 5
4 Materials and Methodology 6-8
5 Results and Discussions 9-13
6 Conclusions 14
7 Future Aspects 15
8 Outcomes 16
9 References 17-19
 LIST OF FIGURES

Figure name Page

no
Common causative agent of wound infection 1
Preparation of titanium dioxide nanoparticles by biosynthesis method. 7
Synthesized TiO2 nanoparticles, Nanoparticle image in SEM 9
Antimicrobial test of TiO2 nanoparticles against E.coli & S.aureus 11
Antimicrobial assay of bioactive components from P. americana 12
against E.coli & S.aureus
Minimal inhibitory concentration of S.aureus 12
Applied for MOE’s INNOVATION CELL 16

Presented in 5th International Conference on "RecentInnovations in 16

Science & Technology (RIST 2023)"

LIST OF TABLES

Table name Page

no
Components of P. americana identified from LC-MS analysis 10
Minimal inhibitory concentration of TiO2 NPs against E.coli & S.aureus 11
Inhibitory effect of cockroach extract against E.coli & S.aureus 12

ABBREVIATIONS

SEM Scanning Electron Microscopy

LC-MS Liquid Chromatography- Mass Spectrometry

E.coli Escherichia coli

S.aureus Staphylococcus aureus