EVOLIS Twin Plus User Manual EN (English)
EVOLIS Twin Plus User Manual EN (English)
EVOLIS Twin Plus User Manual EN (English)
TM
User Manual
REF 93502-m-EN
LOT V1.00 – 0805
IVD
We reserve the right to make changes in the course of technical development without prior
notice.
Copyright - Software
The software for the EVOLISTM Twin Plus analyzer has been licensed
to Bio-Rad for worldwide distribution. The EVOLISTM Twin Plus
software is the intellectual property of STRATEC Biomedical Systems
AG. Intellectual property rights shall remain with STRATEC
Biomedical Systems AG.
You are entitled to use the EVOLISTM Twin Plus software at your
place of work only.
Bio-Rad
3, boulevard Raymond Poincaré - B.P. 3
92430 Marnes-la-Coquette - France
Table of Contents
1 System Overview.............................................................9
1.1 Instrument Description.......................................................................9
1.2 Software Overview ............................................................................11
3 Advanced Information...................................................35
3.1 Assays................................................................................................36
3.2 Barcodes ............................................................................................38
3.3 Samples..............................................................................................40
3.4 Reagents and Controls.....................................................................41
3.5 Non Bio-Rad Kits...............................................................................46
3.6 Dilution Plates and Diluents.............................................................47
3.7 Pipetting Tips ....................................................................................48
3.8 Logs....................................................................................................49
3.9 Schedule ............................................................................................51
3.10 Multi-Assay per Plate........................................................................52
4 Troubleshooting ............................................................75
A. Appendices ....................................................................81
A 1. Technical Specifications ..................................................................82
A 2. Accessories and Consumables .......................................................84
A 3. Data Label ..........................................................................................87
A 4. Symbols and Abbreviations.............................................................88
Important Notice
This manual contains all information needed:
• to operate the EVOLIS Twin Plus system on a daily basis with Bio-Rad
predefined assays,
• and to perform regular maintenance routines.
All first-time users should have read this manual carefully before operating
the EVOLIS Twin Plus system.
Users must not perform any operation not described in this manual.
If in doubt, please contact Bio-Rad Technical Support before performing the
intended operation.
Typographical Conventions
Notes are indicated by this symbol and printed in bold-face type. Non
compliance with these recommendations may result in the test results
being invalidated or unreliable results or having to abort a run.
Menu items Are printed in bold-face type, separated from the menu by a vertical line.
Example: select the File | New menu item.
Software messages Are printed in italics when quoted in the general text.
Example: "Duplicate patient ID [...].
* Remove patient tubes and verify which one should be used."
Safety Instructions
The user has to observe the information and warnings contained in this
manual in order to ensure safe operation of the instrument.
• If you can see that the unit has become unsafe to use, switch it off
and disconnect it from the power supply.
• If liquid gets inside the instrument, disconnect the power cord and
clean the respective parts using appropriate cleaners (in particular
those spots that are crucial to operation).
• Spare fuses must match the values specified by the system
manufacturer.
• The tests and maintenance work recommended by the
manufacturer should be performed to make sure that the operator
remains safe and that the instrument continues to function
correctly.
• Any service and maintenance work not described in this User
Manual must be performed by Bio-Rad Service Engineers.
1 System Overview
➊
➋
➐ ➎
➏
➒
➑ ➍
➓
Touchscreen
System cover
Wash solutions, trap flask and vacuum flask
Sample and reagent loading unit
Pipettor
Microplate loading and pipetting position
Back compartment (washer, incubators and photometer)
Tip racks
Dilution plates / large reagent bottles (diluent)
Tip evacuation slide (& tip waste bag [not visible])
Power switch and connection panel (not visible)
Main toolbar
Worklist toolbar
<File> <Stop>
<New Worklist> <Load Tips>
<Open> <Utilities>
<Save> <Windows>
<Print> <Help>
<Import>
<Worklist
<Scroll> (Page Up)
Parameters>
➊ ➋
1. Make sure the system liquid container is full or almost full and correctly
connected to the system.
2. Make sure the liquid waste container is empty and connected to the
system.
See also Prepare system-liquid, Decontaminate waste container, see Section 3.20.
2.1.2 Start-Up
1. Make sure the instrument cover is closed; switch on the system with
the switch located on the right-hand side of the instrument. On the
touchscreen, WindowsTM starts automatically.
2. On the screen, double-click on the EVOLIS Twin Plus icon to start the
EVOLIS Twin Plus software.
3. The Log-On dialog box is displayed. Click <Log-On>.
Figure 4: Logging in
4. In the Make a Selection dialog box, all registered users are displayed.
Click on your user name.
5. Use the touchscreen keyboard to enter your password, then click
<OK>.
6. The instrument initializes and all modules are checked. At the end of
the initialization process the Selftest window is displayed.
If all instrument modules have a "Passed" status, you can safely start
working with the system.
Check visually the quality of the samples you are intending to process
(clots, foam…). To avoid clots, proper treatment (e.g. centrifugation)
of patient samples is recommended.
4. On the EVOLIS Twin Plus system, one of the yellow LEDs in front of
the sample and reagent unit should be lit. If no LED is lit, reinitialize the
barcode scanner by inserting a sample rack on any track (all the way
down the track) and removing it immediately.
5. Take the first sample rack you want to load and insert it on the track
marked by the yellow LED. Push it in evenly so that all sample
barcodes can be read by the integrated barcode scanner.
If the rack has been correctly read, a brief sound is heard and the
yellow LED on the next track lights up.
If the rack has not been correctly read, the sound is repeated three
times and the first LED keeps flashing. Pull out the rack and insert it
again.
6. If the rack has been correctly read, wait until the following dialog box is
displayed.
In this dialog box, the sample IDs read by the barcode scanner are shown
on the left-hand side and the assay names are listed in the top buttons.
Checkmarks show which tests are selected for each loaded sample. If there
7. Click the green arrow to scroll down the list of loaded samples and
make sure all sample IDs have been correctly read.
1. In the upper toolbar, click the <New Worklist> button. This opens the
Set-up Panel dialog box showing the worklist automatically suggested
by the system.
➊
➋
Plates zone
Plate Layout
Assays zone
The Plates zone shows how many plates are included in the worklist.
The Assays zone shows the name(s) of the assay(s) assigned to this
plate and above, the actual plate layout is presented.
2. Click successively on each plate listed in the Plates zone to see how
the worklist is organized (plate layouts and tests programmed on each
plate).
3. List and prepare the Bio-Rad kits that you will need to process the
assays included in this worklist.
1. Take the kit package you intend to use for the first test. Note the batch
number, printed on the box next to the indication.
2. Click the <Edit Batch Number…> button. In the Make a Selection
dialog box, select the batch number corresponding to the one printed
on the kit box.
The kit Batch Number and the Expiry Date are automatically updated.
3. Click <OK> to validate and repeat these steps for the other kits/tests
included in the worklist.
If the batch number printed on the kit package is not listed in the
Make a Selection dialog box, this means the kit you are intending to
use is the first one from a new batch that has not yet been entered in
the kit lot tracking database. You can click <Add Kit…> to enter it now
as described in Section 3.4.
It is not possible to use two boxes of the same type of kit with
different batch numbers in the same run.
If batch numbers and expiry dates are already listed in the Lot
Specific Values dialog box when this dialog box opens, these are from
the batch used in the last run!
To update this data it is essential that you always click <Edit Batch
Number…> and select the correct batch number from the Make a
Selection dialog box.
Worklist window
Once you have declared the reagent lots for all kits/tests in the Lot Specific
Values dialog box, the worklist window is displayed.
1. Click the various buttons in the first column of the worklist toolbar (in
the left-hand side of the screen) to review the worklist. If something is
incorrect and you need to go back to the Set-up Panel dialog box, click
<Edit Panel…>.
2. Click the <Reagent List> button to display the list of all required
reagents.
3. If you want to print that list (recommended), click the <Print> button in
the upper toolbar.
4. Click the <Start> button in the worklist toolbar to open the Load dialog
box and complete the loading process.
When you click the <Start> button in the worklist toolbar the Load dialog
box opens showing you where to load all required resources.
➍
➋
➌
In the Load dialog box, clicking on any resource displays the details
(name, volume…) of this resource.
For samples and reagents, use the Zoom tool to make clicking easier.
The second part of the reagent list you previously printed (see Figure 11)
indicates which quantity of each solution is required for the planned worklist.
1. Fill each wash solution container with the appropriate solution. The
containers are color-coded (on the screen and on the tubings
connected to the container caps). Fill and reconnect the containers one
at a time to avoid mix-ups. Click successively on each container in the
Load dialog box to display the name of the wash solution you have to
fill in that container. Under default settings, the red-capped container is
reserved for rinse fluid (deionized water).
2. When reconnecting the containers, make sure the level sensors (inside
the containers) and the outside tubing are correctly positioned.
➊ ➋ ➌
After inserting the tip racks, push them down firmly again to make
sure they are level.
Always observe the position of the tip racks defined in the Load dialog
box! Using a short tip instead of a long one may cause splashing and
contamination. Using a long tip instead of a short one may cause the
pipettor to crash and be damaged. Note that the system can be
configured to perform an automatic tip size check. For more
information, please call Bio-Rad Technical Support.
See also Tip management options, Reloading tips during a run, see Section 3.7.
2.4.4 Reagents
a) Before b) After
The first part of the reagent list you previously printed (see Figure 11)
indicates all the reagents required for the planned worklist.
1. Open the kits you prepared for this worklist. Load all reagents and
controls listed in the first part of the list on the reagent racks.
2. Make sure all barcode labels face right.
3. Remove the bottle caps.
4. Insert the reagent racks one by one on the EVOLIS Twin Plus
instrument on the tracks marked by a flashing yellow LED.
If an error message is displayed when you insert the large reagent rack
(rack $1), see Section 3.4.
When all reagents are loaded, in the Load dialog box, all reagents
should be shown in the rack loading unit. The Unallocated resources
corner (top right) should be empty (see above Figure 16 b).
5. Check visually once again that all resources have been loaded as
depicted in the Load dialog box and click <OK>.
Before going further, make sure all caps on reagent bottles and control
vials have been removed!
If you intend to re-use controls and reagents later, store all caps
appropriately to avoid any mix-up when recapping and storing the
bottles.
See also Non-barcoded reagent bottles, Unreadable barcodes, see Section 3.2.
Size-adaptors for reagent bottles, Control racks, Unstable reagents, see
Section 3.4.
Large reagent bottles (diluents), see Section 3.6.
When you click <OK> in the Load dialog box, the Load Plate dialog box is
displayed. The system assigns a default name to each plate:
"Plate NYYMMDDXX"
1. To enter a customized Plate ID, click the <Plate ID…> button and enter
a new plate ID via the keyboard. The plate ID you enter will replace the
"Plate N" part of the default name. The system will still add the
"YYMMDDXX" tag to your customized name.
For example, if you enter "HIV_" instead of "Plate 1", the final Plate ID
will be: "HIV_YYMMDDXX".
2. Take the microplate out of the respective Bio-Rad kit. Make sure the
microplate includes enough strips (at least as many strips as shown in
the Plate Layout of the Load Plate dialog box.).
3. Lift the instrument cover and load the microplate into the plate support
at the back of the instrument deck.
A1 4. Make sure the A1 corner of the microplate is at the rear right and
corresponds to the A1 mark on the plate support.
Push the microplate down firmly so that its surface is perfectly
horizontal.
5. Click <OK>. The microplate moves in. The Load Plate dialog box is
closed then reopens to let you load the next microplate.
6. Repeat these steps for each microplate included in the worklist.
Always load full strips! Even if you use microplates with breakable
wells and only some wells are required on some strips, do not remove
unrequired wells.
See also Microplate identification (no barcode identification), see Section 3.2.
Multi-assay per plate, see Section 3.10.
2.5 Run
Once you click <OK> after loading the last microplate, the run starts
automatically.
You can nevertheless follow what the system is currently doing by toggling
between these different views.
Log view The Log view records all processing events and actions as they
are performed (see Section 3.8).
You can review the result report on the screen (use the scroll buttons in the
left-hand side toolbar) or click the <Print> button (upper toolbar) to obtain a
printout.
An "auto-print" option is available. If this option is enabled, the result report is
printed automatically when displayed on the screen. For more information on
this option and how to enable it, please call Bio-Rad Technical Support.
When using Bio-Rad predefined assays, the result report always includes
the following sections.
➊ ➐
➋
➌ ➑
➍
➎
➏
All results on the plate are invalid. You must retest all samples on that
plate!
If this section includes any of these warnings, all results on the plate
must be individually reviewed and validated by the laboratory
supervisor.
1. Once you have reviewed and validated the results, click the <Export
Results> button in the left-hand side toolbar to export the results to the
LIS (or to another folder or network location).
If you do not click the <Export Results> button, when you try to close
the displayed result file, the system asks you again if you want to
export the results.
2. Click <Yes> to export the results and close the file, click <No> to close
the file without exporting the results.
You can now either start a new run or shutdown the system as described in
the following Section.
1. Make sure all processing has ended. Perform the end of run
maintenance described above.
2. Close the EVOLIS Twin Plus software. Select File | Exit from the
menu bar or click the X icon in the top right-hand corner of the
EVOLIS Twin Plus software window.
3. Exit Windows.
Always exit the EVOLIS Twin Plus software and the Windows software
before switching off the system!
6. Open the instrument cover and wipe the tip adapter (pipettor head)
using a soft lint-free cloth moistened with 70% Ethanol.
7. Inspect the instrument (inner and outer surfaces) for stains and spills. If
necessary, clean as described in Section 3.20.
3 Advanced Information
3.1 Assays
3.2 Barcodes
3.3 Samples
3.4 Reagents and Controls
3.5 Non Bio-Rad Kits
3.6 Dilution Plates and Diluents
3.7 Pipetting Tips
3.8 Logs
3.9 Schedule
3.10 Multi-Assay per Plate
3.11 Pipetting Errors
3.12 Pausing a Run / Emergency Stop
3.13 Continuous Loading
3.14 Flags
3.15 Recalculate Results
3.16 Import
3.17 Export
3.18 Users Rights and Passwords
3.19 Demo Mode and Connections
3.20 General Maintenance Tasks and Maintenance Schedule
3.1 Assays
The EVOLIS Twin Plus system is supplied with pre-defined assay protocol
files (APF). Under default settings, these assay files (files with an *.asy
extension) are stored in the C:\BioRad\2PS\Resources\APF directory.
2. In the Make a selection dialog box, select the "Assay file (*.asy)" icon
and in the next dialog box, select the assay file you want to review.
3. Use the scroll buttons to scroll down the list or the <Filter…> button to
find the file you need more easily.
For example: enter "hiv" with the keyboard to display all assay files with
"HIV" in the assay file name.
4. A password prompt is displayed if the assay file is password protected.
Click <Don't Know Password>. The assay file is displayed.
Review it on the screen or click the <Print> button in the upper toolbar to
review the printout.
APF upgrades
New APF (Assay Protocol Files) versions are regularly released. These
include new Bio-Rad pre-defined assays but also new versions of existing
assays, validated for the most recent software version.
The best way to avoid problems is to install new software and APF versions
as soon as possible. Using old software versions with newly released APF
files or vice-versa is either impossible or may lead to errors and/or loss of
guarantee.
If you want to upgrade your system or inquire about the latest available
versions, call Bio-Rad Technical Support.
3.2 Barcodes
Sample barcodes
It is recommended to use only barcoded sample tubes. When installing the
system, your Bio-Rad Service Engineer will help you make sure the barcode
labels you generally use on sample tubes are adequately read by the
system's integrated barcode scanner.
In the result report (in the Combined report), the respective sample will
have a "ManID" flag to indicate that it has been manually identified.
Reagent barcodes
It is recommended to use only barcoded reagent bottles. Most Bio-Rad kit
bottles are pre-barcoded. For non-barcoded reagent bottles, Bio-Rad
supplies separate sets of barcode labels for each type of kit. Please order
the appropriate barcode labels and attach them to the bottles before use.
Microplate barcodes
The EVOLIS Twin Plus system is not equipped with a barcode reader for
microplates. Microplates can only be identified manually by the operator.
3.3 Samples
Duplicate samples
If you load two samples tubes with the same barcode, an error message is
displayed.
Remove one of the sample tubes or use another barcode on one of the
tubes.
Standard racks
Large reagent rack (Rack code $1) 8 reagent bottles (Ø 39 mm x 2 et
Ø 35 mm x 6) can be loaded on this rack. For diluent bottles with a diameter
greater than 39 mm, see Section 3.6.
Control rack (Rack code $2) 16 control vials can be loaded on this rack.
This rack is specially designed (with height-adaptors on the bottom of each
loading position) to make it possible to load directly the small control and
With calibrator vials included in most Bio-Rad kits.
height-adaptors
Special cases
Rack for controls transferred into tubes (Rack code 3) This rack
(without height-adaptors on the bottom of individual loading positions) is
intended for controls that have been transferred into tubes (identical to
Without sample tubes). Control transferred into tubes should not be loaded on
height-adaptors
ordinary sample racks (Rack code $T).
Adaptors for small diameter bottle Small diameter reagent bottles
(Ø 25 mm) can be loaded on a large reagent rack ($1) when using special
size-adaptors provided with the system.
"The rack has not been positioned in the sample rack in a manner
compatible with the grid.
* Please replace the rack in another track."
In that case, click <OK>, remove the rack, wait for another yellow LED to
light up indicating on which track to reload the rack.
1. Select the Utilities | Add Kit… menu item. This opens the Kit
Information dialog box.
2. With the handheld scanner, scan the main kit barcode on your kit box.
Assay Name
Batch Number
Expiry Date
Product Code
3. All general kit information (except the kit expiry date) is automatically
entered. Click the <Edit…> button next to the Expiry Date field.
4. Use the touchscreen keyboard to enter the expiry date, then click <OK>.
5. Click <Scan kit Bottles…>. This opens the following dialog box. The Kit
drop-down list shows the batch number of the kit package you have just
entered.
6. Open the kit package and load all reagents and controls on the reagent
racks.
7. Load the racks one by one on the EVOLIS Twin Plus instrument on
the tracks marked by a flashing yellow LED. The individual reagent
barcodes are read and the corresponding data is displayed in the Scan
Kit Bottles dialog box (Column 1 = Reagent ID, Column 2 = Lot
number of individual reagent, Column 3 = Individual reagent expiry date
[Date format = DDMMYY]).
8. Click <Close> to close the Scan Kit Bottles dialog box. In the Kit
Information dialog box, the complete kit lot data is now entered.
9. Click <OK> to save and close the Kit Information dialog box.
If you exchange reagent bottles between kits and the system cannot find the
expected barcodes, a message is displayed:
The message tells you the expected ID and the scanned ID. A new message
is displayed for each "unexpected" reagent (i.e. reagent for which the
scanned barcode ID does not correspond to the expected barcode ID).
Click <OK> to close the message. Pull out the reagent rack and check the
reagent(s) mentioned in the message.
If you can find the correct reagent(s) with the expected barcode ID, reload
the rack with only expected reagents.
If you are unable to load the reagent bottles with the expected barcodes, you
can, if necessary, turn the incorrectly labeled bottles so that the unexpected
barcodes cannot be scanned, reload the rack and assign these reagents
manually in the Load dialog box The system then assumes that the
expected reagent has been loaded but reports the manual assignment in the
Result Report.
In the result report (see Section 2.6.1), the Expected kit components
section allows you to track after the run, which reagent lots were used for
each test. Manually allocated reagents are also shown in the Important
warnings section.
External controls
External controls (i.e. controls other than those provided in the Bio-Rad kits)
may be added to any plate.
For more information on this function, please call Bio-Rad Technical
Support.
This manual describes only how to use the EVOLIS Twin Plus system to
process Bio-Rad kits. If you want to process non-Bio-Rad kits, please ask
Bio-Rad Technical Support for assistance. Special conditions may apply.
2. Before loading the dilution plate, make sure that a metal plate is
inserted in the compartment in which you intend to load the dilution
plate.
3. Load the dilution plate. Push the microplate down firmly so that its
surface is perfectly horizontal.
Use only the pipetting tips specially supplied by Bio-Rad for the
EVOLIS Twin Plus system!
When installing the system, your Bio-Rad Service Engineer will configure
the system according to your preferred option. In the Load dialog box (see
Section 2.4), full tip racks are displayed in brown (for 300 µl tips) or gray (for
1100 µl tips), incomplete tip racks are displayed in red (for both tip sizes),
see Section 2.4.3.
3.8 Logs
Log files record in real time the steps of a run as they are performed.
Colors
Different colors are used in the log.
The logs of all runs performed on the same day are aggregated in the same
log file. By default, log files are saved in the
C:\BioRad\2PS\Resources\Event_log directory. They have a (*.log)
extension and the file name corresponds to the date on which the run(s) was
(were) performed:
e.g. "20070228.log" (YYYYMMDD)
To view the log file for the current day (during of after a run):
1. In the worklist toolbar (left-hand side of the Worklist window), click the
<Log> button.
2. In the Make a selection dialog box, select the log icon and in the next
dialog box, select the log file for the day of the run you want to
investigate.
3. Once the log file is open, a <Filter…> button (in the left-hand side
toolbar) allows you narrow down your search to a specific worklist, a
specific plate and a specific sample.
When the log file is created (i.e. during a run), the scripting is done so that
the current step is always at the top while earlier steps are further down. But
when you open a formerly saved log file, the daily chronological order is
rearranged from start-up to shutdown.
Use
The log is an important document. It can:
• Be followed while the run is being processed so that you can react
quickly to correct any problem.
• Be used, after the run is over, to check whether all steps have been
properly performed. If, for example, some results are flagged, the log
enables you to understand why.
• Be reviewed at a later date to check how the run was processed.
The wording used in the log file is generally simple and descriptive, making it
easy to follow for any operator after minimal use of the system. If you require
assistance in understanding a particular log file, you can copy the file (from
the C:\BioRad\2PS\Resources\Event_log directory) and mail it to your Bio-
Rad Service Engineer.
3.9 Schedule
Schedule view
Once a worklist is defined, the Schedule view allows you to see precisely
how the run will actually be processed and when reloading will be allowed or
required. During the run, the vertical time bar shows which step is currently
being performed.
➌ ➊
➍ ➋
➎
The EVOLIS Twin Plus system allows you, under certain conditions, to
combine several assays on the same test plate, e.g. Toxo IgG and Toxo
IgM.
If you import worklists / test orders from the LIS and you use barcoded
samples, the combination of several assays per plate is managed
automatically by the system. When suggesting a suitable worklist for the
samples you loaded, if the assays are compatible and the number of
samples is not too great, the system always tries to combine as many
assays as possible on each plate.
For the operator, performing a run with several assays per plate is very
similar to an ordinary run.
2. When you reach the Load Plate dialog box, pay special attention to the
plate layout and particularly, the number of strips used for each test.
3. You will need to take a microplate from each of the two (or more) kits
and rearrange the correct number of strips for each test in one
microplate.
Be careful not to mix-up strips! When microplates supplied in Bio-
Rad kits have removable strips, a 2-digit code is engraved on each
strip for easy identification.
Always load full strips! Even if you use microplates with breakable
wells and only some wells are required on some strips, do not remove
unrequired wells.
Always start the second (third) assay on a new strip (default setting). If
you want to avoid "loosing" some wells, please ask Bio-Rad Technical
Support for assistance as to how to solve this problem.
4. When the result report is displayed (once the processing has ended),
the results corresponding to each assay are displayed one after the
other, with the same order that they had on the plate (i.e. full results for
Assay 1, then full results for Assay 2...).
5. Similarly, when the results are exported, the results for all assays
processed on the same plate are included in the same result file.
Samples
When using Bio-Rad assays, if a pipetting error occurs on a sample, the
processing will continue normally but the error is traced in the log file and the
sample will be flagged in the Combined report according to the type of
problem detected: clot, insufficient liquid, no liquid… (see Section 3.14).
Reagents
When using Bio-Rad assays, if a pipetting error is detected on a reagent, a
message is displayed:
Pre-run checks
Reagent volume check (optional but recommended)
The pipettor controls that each bottle contains enough reagent for the
planned worklist.
Sample volume check (optional)
The pipettor controls that each sample tube contains enough liquid for the
planned worklist.
Tip size check (systematic)
The pipettor controls one tip of each tip rack to make sure that long tips and
short tips have been loaded as requested in the Load dialog box.
During the run
Verify Dispense
Photometric control of adequate reagent or sample dispense.
Aspirate check / Liquid level detection
When pipetting out of a container, the pipettor controls that the liquid level
drop corresponds to the aspirated volume.
Pressure monitoring
Barometric control on aspirate steps.
Click the <Stop> button in the upper toolbar to pause the run. The following
dialog box is displayed.
You can then choose to restart the processing (<Resume>), to select one or
more plates and click <Abort Plate> or abort the complete worklist. If you
click <Abort Plate> or <Abort Worklist>, a confirmation message will be
displayed.
Emergency stop
In extreme emergencies it is recommended to simply unplug the system.
In all other cases, it is preferable to pause the system as described above,
abort the worklist and shutdown normally as described in Section 2.7.2.
Continuous Loading is the process by which new patient samples and new
test plates are reloaded while the system is already running. This is possible
on the EVOLIS Twin Plus system under certain conditions.
2. Check the LEDs at the front of the sample loading unit. You can
remove all the racks for which the LEDs are flashing.
10. Case 2) If you are not or no longer within a "brown section", the
system displays a message stating:
You will be able to add plates in […] minutes. Please try again then.
11. Wait until the specified time has elapsed (or until the time bar enters a
brown section in the Schedule view), then click the <Edit panel…>
button again and follow the steps described above.
When loading the resources for the new samples, it is important that
you load them as quickly as possible so that the processing is not
paused for too long.
If some of the plates of the initial worklist are already fully processed
when you are ready to reload additional plates, the system
automatically brings them forward to the loading/unloading zone so
that you can remove them before loading the additional plates.
3.14 Flags
Flagged results are not necessarily wrong results. A flag indicates that
something happened during the run that may have affected the result on this
patient sample.
The software uses 12 general flags and 6 pressure monitoring flags to give
an indication of the type of problem encountered.
SplRem Sample rack removed. This flag is used if a sample rack has been removed
before it had been completely pipetted. No results are calculated for
samples that had not yet been pipetted at that stage.
RgtRem Reagent rack removed. This flag is used if a reagent rack has been
removed during processing. This does not affect result calculation (the
results are calculated).
ManID Manual ID. This flag is used if a sample ID has been manually assigned. .
This does not affect result calculation (the results are calculated).
If a manually assigned sample is used for several assays (through direct
pipetting or through pipetting of the same predilution made from this
sample), the ManID flag is included in the Result Report for each assay.
NoLiq No liquid detected. Results for flagged samples are not calculated.
InsLiq Insufficient liquid detected. Results for flagged samples are not calculated.
Clot Clot detected. Results for flagged samples are not calculated.
PipErr Pipettor hardware error. Results for flagged samples are not calculated.
ManPip Manually pipetted resource. This flag is used when controls or samples have
been manually pipetted into the test plate. This does not affect result
calculation (the results are calculated).
VCFail Validation criteria failure. Results for flagged samples are not calculated.
VDFail Verify dispense failure. This flag is used when a reagent / sample / control
has not been correctly dispensed into a well. Results for flagged samples
are not calculated.
IncKo Incubation overrun. This flag is used when there is a discrepancy between
the incubation temperature / time actually observed during a run and the
incubation temperature / time defined in the assay. Results for all samples
on an incorrectly incubated plate are not calculated.
REAG EXP Reagent Expired. This flag is used when a reagent was used after its expiry
date. When an expired reagent is loaded and identified, the user is warned
that the expiry date has been reached/exceeded but can choose to override
the warning and still use the reagent for the run.
This does not affect result calculation (the results are calculated).
P_max_high, Pressure monitoring flags. These flags can be reported only if the pressure
P_min_low, monitoring function is enabled. In case of recurring pressure monitoring
P_stop_high, flags, call Bio-Rad Technical Support.
P_static_high, P_max_high Aspiration pressure too high Clot
P_static_low, P_min_low Aspiration pressure too low Foam or air
P_mean_low P_stop_high Pressure at pump stop too high Clot
P_static_high Static pressure too high Clot
P_static_low Static pressure too low Foam or air
P_mean_low Mean pressure too low Foam or air
flagged (displayed in red) This flag replaces multiple flags when results in the
Combined Report are displayed in Matrix format.
When a Result report is displayed, the <Outliers…> button (in the left-hand
side toolbar) allows you to manually remove from the results some OD
values which you think are not consistent with the test or conversely to
restore a value which has been automatically removed from the calculation
by the software (flagged sample).
You cannot edit the values but only remove or restore them. A removed
value is displayed crossed out.
1. To remove (or restore) a value, select it with the mouse and then click
the <Remove> (or <Restore>) button.
2. Click <OK>. The program returns to the result report. Removed (or
restored) wells are listed at the bottom of the General information and
Important warnings section.
To then recalculate the results without the removed values or with the
restored values, click the <Recalculate> button (in the left-hand side
toolbar).
The new result report includes the following comment: "WARNING! Results
have not been processed using the original assay."
If several assays were processed on one plate, you can view the DO values
for each assay by clicking the <Assay Protocol> button (in the top left
corner of the dialog box) and selecting the assay you want. The <Reading>
button shows the wavelength(s) used for the reading.
This possibility can only be used for samples with the following flags:
SplRem (Sample rack removed), VDFail (Verify dispense failure) or
IncKo (Incubation overrun).
Results that have been eliminated because of NoLiq (No liquid
detected), InsLiq (Insufficient liquid), Clot (Clot detected), PipErr
(Pipettor hardware error), VCFail (Validation criteria failure) cannot be
recalculated!
To do so:
1. In the original Result Report, display the Combined Report part.
2. Check the flagged samples.
3. If you want to recalculate some of these flagged samples, note their
locations on the plate (layout labels).
4. Open the Outliers dialog box and restore the corresponding wells
(layout labels) as described above.
5. Recalculate the Result report as described above.
6. In the recalculated Result report, the selected flagged results are now
calculated but the original flags remain.
3.16 Import
When installing the system, your Bio-Rad Service Engineer will configure
the communication links and import process (file format, file polling
interval…) so that the EVOLIS Twin Plus can import and interpret test
orders sent by the LIS (Laboratory Information System).
The import process is then performed automatically and does not require
any user intervention.
When test orders are correctly imported, when you load barcoded samples
on the instrument, the Patient Editor is displayed with checkmarks
indicating which tests are required for the loaded samples as described in
Section 2.2.
If there are no checkmarks in the Patient Editor when it opens, this can
mean several things.
Case 1 – Your test order files include only sample IDs but no information on
the tests to be performed (e.g. in situations where all samples are
systematically tested with the same test panel). In that case:
1. Click successively the <None> buttons located above the table and
select an assay for each column.
2. Add checkmarks manually by clicking in the appropriate table cells. Do
not forget to click the green arrow button to scroll down to the bottom of
the table.
3. When done, click <Close>.
Case 2 – No test order for the respective sample IDs was sent by the LIS or
no test order for the respective sample IDs was received by the EVOLIS
Twin Plus. In that case:
1. Click <Close> to close the Patient Editor dialog box.
2. Click the <Import> button in the upper toolbar to manually force an
import process.
3. Reload the sample rack on the track marked by a yellow LED and wait
until the Patient Editor is displayed again.
If the Patient Editor is still blank (no checkmarks), please contact your LIS
administrator. If your LIS administrator cannot solve the problem, please call
Bio-Rad Technical Support.
3.17 Export
When installing the system, your Bio-Rad Service Engineer will configure
the communication links so that the EVOLIS Twin Plus result files can be
exported to the LIS (Laboratory Information System) or to another folder or
network location.
Note however that the export process is not automatic. Each result file has
to be exported individually by the operator (see Section 2.6.2).
[Results]
Patient ID|Assay|Well|Flag|Value|S/CO|Result
""|"HIV Ultra Ag-Ab 1P BR V13"|"A1"|""|"2,0650"|"6,1950"|"PC Ag1"
""|"HIV Ultra Ag-Ab 1P BR V13"|"B1"|""|"2,1860"|"6,5580"|"PC Ab1"
""|"HIV Ultra Ag-Ab 1P BR V13"|"C1"|""|"0,1380"|"0,4140"|"NC1"
""|"HIV Ultra Ag-Ab 1P BR V13"|"D1"|""|"0,1260"|"0,3780"|"NC2"
""|"HIV Ultra Ag-Ab 1P BR V13"|"E1"|""|"0,1360"|"0,4080"|"NC3"
"000001"|"HIV Ultra Ag-Ab 1P BR V13"|"F1"|"ManID"|"0,1160"|"0,3480"|"NEG"
"000002"|"HIV Ultra Ag-Ab 1P BR V13"|"G1"|"ManID"|"0,1080"|"0,3240"|"NEG"
"000003"|"HIV Ultra Ag-Ab 1P BR V13"|"H1"|"ManID"|"0,1770"|"0,5310"|"NEG"
"000004"|"HIV Ultra Ag-Ab 1P BR V13"|"A2"|"ManID"|"0,9000"|"2,7000"|"REACTIVE"
"000005"|"HIV Ultra Ag-Ab 1P BR V13"|"B2"|"ManID"|"0,8150"|"2,4450"|"REACTIVE"
"000006"|"HIV Ultra Ag-Ab 1P BR V13"|"C2"|"ManID"|"0,6620"|"1,9860"|"REACTIVE"
"000007"|"HIV Ultra Ag-Ab 1P BR V13"|"D2"|"ManID"|"0,4760"|"1,4280"|"REACTIVE"
"000008"|"HIV Ultra Ag-Ab 1P BR V13"|"E2"|"ManID"|"0,3710"|"1,1130"|"REACTIVE"
"000009"|"HIV Ultra Ag-Ab 1P BR V13"|"F2"|"ManID"|"0,2240"|"0,6720"|"NEG"
[…]
Re-export results
If you try to re-export results that have already been exported, the following
message is displayed.
"These results have already been exported to the LIMS.
Are you sure you want to export them again?"
Click <Yes> to re-export, click <No> to stop the second export.
Supervisors Full access group. Members of this group are allowed to use all the
functions available on the system. At least one supervisor is required per
system.
Users Restricted access group.
When installing the system, your Bio-Rad Service Engineer will create new
users and preset the user rights of each group of operators as requested to
reflect your laboratory organization.
If you need to reset your password because you have forgotten it, you first
have to seek the assistance of a supervisor or of another user who is
allowed to Administer Users and who can delete your former password.
5. Click <OK> to close the message, then click <OK> again to close the
Users tab.
The next time you log in under your own user name, you have to reset a new
password as described above.
Demo mode
If you want to use the EVOLIS Twin Plus software without the instrument,
start-up as usual (see Section 2.1.2) but when you reach the Log On dialog
box, select the <Demo mode> button (the green dot "lights up") before
clicking the <Log On> button.
Select Demo mode also if you install and use the EVOLIS Twin Plus
software on a separate computer.
Connections
A connection panel is located on the right-hand side of the system, above
the power switch.
System-liquid preparation
1. To prepare a full container of system-liquid, add 2 ml of Tween 20 to 10
liters of deionized water.
The screw cap and sensor part may be wiped with a cloth moistened with
RIVASCOP® (diluted in water at 0.4% - 4 ml per liter).
Surface decontamination
The following areas:
• cover and handle,
• outer panels (avoiding connection panel – see Section 3.19),
• inner instrument deck (worktable),
• rack loading unit and upper grid,
…can be cleaned with any bactericide, virucide and fungicide hospital-grade
disinfectant. To prepare and apply the disinfectant (dilution, spray, wait,
wipe...) refer to the instructions prescribed by the product manufacturer.
DO NOT try to lift or remove the inner instrument deck (worktable) and
do not try to move the instrument itself! If this happens accidentally,
call your Bio-Rad Service Engineer and do not use the system before it
has been realigned.
Do not use bleach or disinfectant which may damage metal parts. Do
not use disinfectants containing alcohol or acetone for Plexiglas
surfaces (cover).
Do not use the system until it has been checked by your Bio-Rad
Service Engineer.
4. Click <OK> to validate the various dialog boxes until you reach the
Worklist window.
5. Click <Start> and load the required resources (tips, deionized water,
decontamination solution for the monthly procedure, standard flat-bottom
microplate).
6. Start the run and follow the instructions on the screen.
This creates backup copies of all current files that are not part of the
standard installation and stores them, under default settings, in an individual
directory created in the C:\BioRad\2PS\Backup directory. The name of the
individual system backup directory is formed as follows: "SYSyyyymmddnn"
(y = year, m = month, d = day, n = number of backups done on that day). A
new individual directory is created each time you launch a new backup
process (the previous backup is not overwritten).
6. In the Set-up Panel dialog box, click <Add Plate…> again to create the
second plate.
7. In the Set-up Panel: Plate dialog box, click <Add assay…> and select
the CTRL Washer Aspirate ETP BR.asy file.
8. Do not add any samples. Just click <OK> to validate the various dialog
boxes until you reach the Worklist window.
9. Click <Start> and load the required resources: tips, deionized water,
reagents (color solutions from the PE Kit) and tap water bottles,
standard flat-bottom microplates.
10. Start the run and follow the instructions on the screen.
11. When the result report for each plate is displayed, review the Validation
criteria section (see Section 2.6.1) for each assay (2 assays on the first
plate!). If any of the Validation criteria sections includes a FAILED
mention (in bold red type), call Bio-Rad Technical Support.
You can run these assays at any other time also if you suspect a malfunction
of the pipettor or of the washer.
A complete performance evaluation check of all instrument modules (plate
transport, photometer, pipettor, washer, and incubators) will be performed by
your Bio-Rad Service Engineer as part of the preventive maintenance visits.
Maintenance Schedule
Daily Maintenance
Weekly Maintenance
Run the weekly washer maintenance assay Create a worklist for one plate using the weekly washer
maintenance assay and run it (see above).
Clean the instrument surfaces and work areas See above General Maintenance Tasks.
Decontaminate the sample and reagent racks and the Prepare a decontamination solution consisting of 0.4 % (4 ml per
®
tip ejection slide. liter) RIVASCOP and water in a container/sink large enough so
that you can immerse the racks and the slide completely.
Before immersing the racks and slide, set aside 5 ml of the
decontamination solution for the next step (see below).
Let them soak for a minimum of 15 minutes, longer in case of spills
or stains. Overnight if possible.
Rinse them thoroughly under tap water and allow to dry.
Decontaminate the pipettor wash station Pour the 5 ml of the decontamination solution you set aside in the
previous step into the pipettor wash station.
Let it soak for a minimum of 15 minutes (overnight if possible). Do
not empty. The liquid will drain automatically when the system is
next reinitialized.
Monthly Maintenance
Run the monthly washer maintenance assay Create a worklist for one plate using the monthly washer
maintenance assay and run it (see above).
Run the monthly performance evaluation assays. Create a worklist for two plates using the monthly performance
evaluation assays and run it (see above).
Decontaminate the system liquid container Empty the container, then fill it with a decontamination solution
®
consisting of 0.4% (40 ml for 10 liters) RIVASCOP .
Without putting the cap back on, let it stand for a minimum of 15
minutes (overnight if possible).
Empty the container and rinse it thoroughly, twice with tap water
and once with deionized water.
Before refilling and reconnecting, inspect the filter (attached to the
cap). If damaged or particularly dirty, replacements can be ordered
from Bio-Rad.
Clean the wash buffer / clean fluid bottles The wash buffer/clean fluid bottles can be either soaked and
washed by hand according to the procedure used for laboratory
glassware or cleaned in a laboratory dishwasher. Use the same
cleaning agents as for laboratory glassware.
Do not autoclave.
Clean the bottles only, NOT the caps and sensors.
4 Troubleshooting
[...] fatal error: System fluid low. During the initialization procedure or Refill the system liquid container (see
* Refill the system fluid container. during the run. Section 3.20). When done, click <OK>.
A kit already exists with product When referencing a new kit lot. If you are scanning the barcode on a kit
code [...] and batch number [...]. box, this means that this kit lot has
* Please enter a unique product code already been referenced in the kit
and batch number. database. Exit the kit database. When
you start a run with this kit, click <Edit
Batch Number…> in the Lot Specific
Values dialog box and select the correct
batch number.
If you are entering the batch number
manually, check that you entered the
correct batch number. If the number you
entered is correct, see above.
Otherwise, try to enter the number
again.
Are the metal plates inserted below The programmed run requires a dilution Make sure you have inserted a metal
the dilution/archive plates? plate to be loaded in a slot where a large plate under each dilution plate (see
diluent bottle was loaded in the previous Section 3.6).
run.
Are you sure you want to abort all of The run is paused and you have clicked Click <Yes> or <No> as required (see
the plates? the <Abort worklist> button Section 3.12).
(confirmation message).
Are you sure you want to ABORT The run is paused and you have clicked Click <Yes> or <No> as required (see
plate [...]? the <Abort plate(s)> button (confirmation Section 3.12).
message).
Are you sure you want to export You clicked the <Export results> button. See Section 3.17.
these results to file and/or LIMS?
Aspirate check failed in reagent [...]. During the run. Liquid level detection See Section 3.11.
error on reagent aspirate step.
If the error persists, please call Bio-Rad
Technical Support.
Aspiration pressure to high APM error (see Section 3.14). The result will be flagged: P_max_high
Aspiration pressure to low APM error (see Section 3.14). The result will be flagged: P_min_low
Barcode error for reagent [...]! During the run. When loading an unstable Check the position of the reagent bottle
* Ensure that the bottle with barcode reagent. on the rack (see Section 3.4).
[...] is positioned correctly.
Clot detected/Aspirate check failed During the run. See Section 3.11.
in [...].
Error on reagent aspirate step.
If the error persists, please call Bio-Rad
Technical Support.
Crash recovery file detected. After power failure. <No>: System is reinitialized. Old
Do you want to try and recover the worklist will be deleted. <Yes>: The
worklist? following message appears:
"Is the system still running?"
<No>: The system initializes the
modules and continues the next worklist
step. <Yes>: The system continues with
the next worklist step.
Error! Reagent [...] has not been During the run. The system prompted you If this message is displayed, you can still
loaded! to load a reagent (e.g. unstable reagent load the required reagent but you will
or reagent to be refilled) and the required need to check the results and the log file
reagent was not loaded within the preset carefully to see if the delay had an
time span. impact. If in doubt, discard the results
and retest the samples!
Expected barcode(s): '(list of all Lot tracking error. Load the correct reagent bottle (from the
reagent barcodes expected for that kit lot you have referenced in the kit
assay) [...]' database - see Section 3.4).
Scanned barcode: '(barcode of first
unexpected reagent)[...]'
Please check the barcode and retry.
Incorrect password. You entered a wrong password. Enter the correct password. Passwords
Try entering the password again. are case-sensitive! If you have forgotten
your password, see Section 3.18.
Insufficient volume of [...] detected. During the run. Volume of reagent is Please make sure that enough reagent
insufficient. liquid is available and click <Refill
bottle>.
Otherwise, click <Abort Plate>.
Insufficient volume of [...] for the During pre-run reagent volume check. Refill the specified reagent bottle.
worklist. Not enough reagent available in the
specified container.
Insufficient volume of [...], position During pre-run wash solution volume Refill the specified container with the
[...], for the worklist. check. Not enough wash solution required wash solution.
available in the specified container.
Lot number [...] does not exist! Lot tracking error. The scanned lot Scan the correct lot number or update
number does not exist. the kit database.
Mean pressure too low APM error (see Section 3.14). The result will be flagged: P_mean_low
No disposable tips left During the run. There are no more tips Load more tips (see Section 3.7).
available.
No information could be found for Lot tracking error. The scanned kit Enter the correct product code.
product code [...]. barcode or the product code you entered
does not exist.
No kit information can be found for Lot tracking error. The batch number Enter the correct batch number or
this batch number. does not exist in the kit database. update the kit database.
No liquid detected for [...] During the run. No liquid for reagent ... is Fill or reload a bottle of the required
detected (this message is displayed only reagent and click <Refill bottle>.
if pre-run reagent volume check is
disabled). Otherwise, click <Abort Plate>.
Note: The current worklist has been Continuous loading. The system allows See Section 3.13.
paused. you to reload now.
Press the 'Start' button when you are
ready to proceed with the new
worklist.
Static pressure to low APM error (see Section 3.14). The result will be flagged: P_static_low
Strip/well verification error! When loading microplates. Add the missing strips or wells (see
* Please ensure that a well has been Section 2.4.5).
inserted into the plate for each assay The microplate you loaded includes an
as shown in the plate load dialog incomplete strip or not enough strips.
box.
System waste FULL. The main liquid waste container is almost Replace the full waste container with an
full. empty one, reconnect and click <OK>.
Decontaminate all liquid waste as
described in Section 3.20.
The disposable tips have been During the tip type detection. System After you have clicked <OK> the
incorrectly loaded. detected large tips instead of small tips or software displays the Load dialog box
vice-versa. again so that you can check and correct
the loading position of the tip racks
(300 µl and 1100 µl tips).
The password for that user has been After deleting a forgotten password. See Section 3.18.
cleared.
They should set a password when
they next log on.
The pipettor is currently busy. The When trying to reload tips during a run. Click <OK> to close the message and
pipettor shall need to be paused try again when the pipettor is inactive
whilst additional tips are loaded. (check the Schedule view of the
Are you sure you want to load worklist).
additional tips now?
The rack has not been positioned in When loading the large reagent rack. See Section 3.4.
the sample rack in a manner Because of the grid above the sample
compatible with the grid. and reagent unit, the large reagent rack
* Please replace the rack in another can only be loaded on certain tracks.
track.
The rack in track [...] is not the same Rack changed during refill bottle or Load the correct rack.
as was originally loaded! unstable reagent loading process.
* Please load rack [...] in track [...].
The reagent ID [...] is already in use This reagent ID is already in the kit Enter a unique reagent ID.
for this kit. Please enter a unique database.
reagent ID.
The system is already running You clicked the <New Worklist> button If you want to add more samples/ plates
another worklist. while the current worklist was not yet to a running worklist, please follow the
Please wait until that worklist has finished. continuous loading procedure described
finished. in Section 3.13.
The worklist cannot be started Kit lot tracking error. The worklist cannot Check that you have correctly
because some reagents have an be started until the error(s) have been referenced the kits in the kit database
incorrect barcode. corrected. (see Section 3.4) and that you have not
* Check and reload the reagents.
exchanged reagent bottles between
different kit packages.
The worklist is currently active. You tried to close the worklist window or Click <No> and wait until the end of the
Are you sure you want to close this to close the software while the current run. If you want to abort the worklist, see
window? worklist is not finished. Section 3.12.
These results have already been You tried to export results that have See Section 3.17.
exported to the LIMS. already been exported.
Are you sure you want to export
them again?
This will delete all patient In the Patient Editor, you clicked the Unless you specifically want to delete all
information and test requests! This <Delete All> button. patient information and test requests, it
action cannot be undone! is recommended to use the <Delete
Are you sure you want to delete all (date)> aor the <Delete (Test)> buttons
patient information? instead of <Delete All>.
You will be able to add plates in [...] During continuous loading, you have to See Section 3.13.
minutes. Please try again then. wait until all current plates are incubating
to add new plates.
A. Appendices
A 1. Technical Specifications
Electrical
Voltage 100V - 240V
Frequency 50 - 60 Hz
Power consumption 320 VA (mean)
Fuse rating 4 AT
Operating conditions
General Indoor use only.
No direct sunlight (may mislead
optical sensors and affect
performance)
Temperature 15 - 30 °C
(storage 5 - 50 °C)
Humidity 30 - 80 % (non-condensing)
(storage 10 - 85 % non-condensing)
Altitude < 2000 m above sea level
Noise level 70 dB (A) at 1 m
Pollution degree 2
Installation class 2
Sample processing
Primary sample capacity 144
Dilution capacity (via tubes/plate) 72/192
Container (diameter & height) 10-16 mm/50-100 mm
e
Sample dilution up to 1/10,000
Reagent processing
Calibrators & controls capacity 16
Reagents capacity 16
Reagents containers Bio-Rad reagent bottles
Reagents identification internal BCR
Lot & expiration management yes
Disposable tips
Carbon tips 300-1,100 μl
Volume (useable) 10-1,000 μl
Liquid level & clot detection capacitive + air pressure
On-board capacity (walk-away) 288 tips (3 x 96)
Processing features
Patient samples (100 μl/well) ~ 16 min/full plate
Reagent (100 μl/well) ~ 4 min/full plate
Dilution (1:10) ~ 23 min/full plate
Single dispense Multi-dispense
Precision (at 20 μl) < 6% CV < 10% CV
Accuracy (at 20 μl) < 10% < 10%
Precision (at 100 μl) < 3% CV < 5% CV
Accuracy (at 100 μl) < 5% < 5%
Incubation specifications
Number of incubators 3 x ambient T
2 x from ambient T +5°C, up to 50°C
Temperature Precision 1.4°C
Temperature Accuracy ± 1.0°C
Washer specifications
Manifold 8 channels
Plate type (bottom shape) flat, U- & V-shaped
Wash buffers 2 x 2 l, 1 x 1 l
Photometer specifications
Reading head 8 channels
Reading range 0-3.5 OD
Filter wheel 8 (equipped with 450, 492, 620)
Linearity (0-2.0 OD) 1%
Precision (0-2.0 OD) 2.5%
Communication
Communication Port RS-232 Serial, USB 2.0, RJ-45
On-line (bi-directional) ASTM or ASCII format
P/N DESCRIPTION
RACKS
93517 Standard sample racks (Rack code $T)
93518 Control rack (Rack code $2)
93519 Large reagent rack (Rack code $1)
93520 Size-adaptor for 15 ml reagent bottles (X 6)
90328 Tray for sample racks
90353 Barcode labels for alternative racks (U, V, W, Y, -3)
REAGENT BOTTLES
89776 Reagent bottle 15 ml (X 72)
89777 Reagent bottle 30 ml (X 72)
89778 Reagent bottle 60 ml (X 72)
89779 Reagent bottle 125 ml (X 72)
93516 Adaptor for 125 ml diluent bottles
402-5900 V-Vial Polypropylene reagent container 23 ml (X 500)
402-5820 Push caps for 23 ml V-Vial reagent containers (X 500)
CONTAINERS
89935 2-liter wash solution container, no cap, no tubing
91381 Ordinary cap for 2-liter wash solution container
89934 1-liter wash solution container, with cap, no tubing
89657 1-liter wash solution container, with cap and tubing (Red)
89661 2-liter wash solution container, with cap and tubing (Blue)
89662 2-liter wash solution container, with cap and tubing (Yellow)
93549 Tray for wash solution bottles
89984 System –liquid container, 10-liter
Spare liquid waste container with ordinary cap, no tubing, 10-
89985
liter
89650 Liquid waste container with cap and tubing, 10-liter
TIPS
89611 Small tips 300 µl (X 17280)
89612 Long tips 1100 µl (X 9600)
89799 Tip waste bag (X 10)
MAINTENANCE
1706531 TWEEN 20 for System-liquid preparation (100 ml)
89645 Rivascop Decontamination Solution (X 1 liter)
PE KIT (Pipettor - Washer Reagent kit for performance
89894
evaluation)
A 3. Data Label
REF XXXXX
SN XXXXXXXXXX IVD
Bio-Rad
92430 Marnes-la-Coquette 2008-01
France
Hz Frequency
A Ampere
Fuse rating
SN Serial number
Manufacturer
Date of manufacture
On the instrument
General hazard
Biohazard
Electric Hazard
In this manual
Biohazard