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    Alp LQ A15 A25 Draft

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    Đỗ Quốc Mạnh
    The document provides instructions for using an assay to measure alkaline phosphatase levels. It details the reagents, calibration, operating conditions, and parameters to input into a computer for two different assays (A_15 and A_25) to measure this enzyme.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Alp LQ A15 A25 Draft

    Uploaded by

    Đỗ Quốc Mạnh
    11 views2 pages
    The document provides instructions for using an assay to measure alkaline phosphatase levels. It details the reagents, calibration, operating conditions, and parameters to input into a computer for two different assays (A_15 and A_25) to measure this enzyme.

    Original Title

    ALP_LQ_A15_A25_DRAFT

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    © © All Rights Reserved

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    The document provides instructions for using an assay to measure alkaline phosphatase levels. It details the reagents, calibration, operating conditions, and parameters to input into a computer for two different assays (A_15 and A_25) to measure this enzyme.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    11 views2 pages

    Alp LQ A15 A25 Draft

    Uploaded by

    Đỗ Quốc Mạnh
    The document provides instructions for using an assay to measure alkaline phosphatase levels. It details the reagents, calibration, operating conditions, and parameters to input into a computer for two different assays (A_15 and A_25) to measure this enzyme.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    ALP

    Fosfatasa alcalina
    p-Nitrofenilfosfato. Cinético. Líquido. DGKC

    PREPARACIÓN Y ESTABILIDAD
    Ref. 41240 o 41242. Ambos reactivos se encuentran listos para el uso.
    A_15 / A_25
    Son estables hasta la fecha de caducidad indicada en el envase.

    CALIBRACIÓN
    Utilizar un suero de calibración adecuado (CFAS Ref. 1002011)

    CONDICIONES OPERATORIAS
    Introducir en la memoria del ordenador los siguientes parámetros:

    ALP A_15 A_25

    1.GENERAL:
    REACTTION TYPE INCREASE INCREASE
    SAMPLE TYPE: SER (SUERUM) SER (SUERUM)
    UNITS: U/L U/L
    # DE REPLICTES: 1 1
    # DECIMALS: 1 1
    ASSAY METHOD: KINETIC KINETIC

    2.PROCEDURE:
    BOCHROMATIC READING
    1 ST WAVELENGTH: 405 405
    2 ND WAVELENGTH:

    3.VOLUMES:
    SAMPLE VOL: 5 ul 5 ul
    REAG1 VOL: 240 ul 240 ul
    REAG2 VOL: 60 ul 60 ul
    CLEAN: 1.2 1.2

    3.TIME:
    READING 1: 252 SEC / C12 255 SEC / C18
    READING 2: 432 SEC / C21 435 SEC / C31
    REAGENT 2: 192 SEC / C9 195 SEC / C14

    4.CALIBRATION.
    CALIBRATOR. SPECIFIC SPECIFIC
    CAL NUMB: 1 1
    CAL REPLICATES: 3 3
    BLK REPLICATES: 3 3
    MULTIPOINT CAL:

    5.CONTROLS:
    # CONTRLS: 1 1
    REJECTION CRITERIA 3 DS 3 DS
    CONTROL TYPE: MULTIPLE. MULTIPLE
    CALCUL METHOD: MANUAL MANUAL
    #BATCH:
    BATCH:
    NAME:
    LIMITS:

    6.OPTIONS
    BLK ABS. LIMIT YES YES
    VALUE:

    Ed. 2018

    SPINREACT, S.A.U. Ctra. Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
    Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com
    ALP

    Alkaline phosphatase
    p-Nitrophenylphosphate. Kinetic. Liquid. DGKC

    PREPARATION AND STABILITY A_15 / A_25


    Ref. 41240 or 41242. Both reagents are ready to use.
    Stable up to the expiration date stated on the label.

    CALIBRATION
    Use a human serum calibrator (CFAS Ref. 1002011).

    OPERATORY CONDITIONS
    Introduce the following data in the computer:

    ALP A_15 A_25

    1.GENERAL:
    REACTTION TYPE INCREASE INCREASE
    SAMPLE TYPE: SER (SUERUM) SER (SUERUM)
    UNITS: U/L U/L
    # DE REPLICTES: 1 1
    # DECIMALS: 1 1
    ASSAY METHOD: KINETIC KINETIC

    2.PROCEDURE:
    BOCHROMATIC READING
    1 ST WAVELENGTH: 405 405
    2 ND WAVELENGTH:

    3.VOLUMES:
    SAMPLE VOL: 5 ul 5 ul
    REAG1 VOL: 240 ul 240 ul
    REAG2 VOL: 60 ul 60 ul
    CLEAN: 1.2 1.2

    3.TIME:
    READING 1: 252 SEC / C12 255 SEC / C18
    READING 2: 432 SEC / C21 435 SEC / C31
    REAGENT 2: 192 SEC / C9 195 SEC / C14

    4.CALIBRATION.
    CALIBRATOR. SPECIFIC SPECIFIC
    CAL NUMB: 1 1
    CAL REPLICATES: 3 3
    BLK REPLICATES: 3 3
    MULTIPOINT CAL:

    5.CONTROLS:
    # CONTRLS: 1 1
    REJECTION CRITERIA 3 DS 3 DS
    CONTROL TYPE: MULTIPLE. MULTIPLE
    CALCUL METHOD: MANUAL MANUAL
    #BATCH:
    BATCH:
    NAME:
    LIMITS:

    6.OPTIONS
    BLK ABS. LIMIT YES YES
    VALUE:

    Ed. 2018

    SPINREACT,S.A.U. Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN


    Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com

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