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    03 Enzymes

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    yezzromaul

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    © All Rights Reserved
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    03 Enzymes

    Uploaded by

    yezzromaul
    5 views3 pages

    Original Title

    03 Enzymes (1)

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    Copyright:

    © All Rights Reserved
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    Download as docx, pdf, or txt
    5 views3 pages

    03 Enzymes

    Uploaded by

    yezzromaul

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    DATE:

    TITLE: Enzymes
    AIM: TO OBSERVE THE ACTION OF THE ENZYME CATALASE
    MATERIALS/APPARATUS: one potato, one scalpel, one ruler, four test tubes, one 250 ml
    beaker, one tripod, one Bunsen burner, one wooden splint, one mortar and pestle, Hydrogen
    Peroxide, Hydrochloric acid, one 10 ml syringe and one pair of forceps.
    METHOD/PROCEDURE:
    (1) Using the scalpel and ruler four pieces of potato were cut into 1 cm³ cubes.
    (2) Using the 10 ml syringe, 2 ml of Hydrogen peroxide was placed into a test tube.
    (3) Forceps were then used to place one potato cube into the test tube and observations were
    made and recorded.
    (4) Using the 250 ml beaker, tripod and Bunsen burner, a water bath was set up.
    (5) Forceps were used to place another potato cube into the water bath and this was left for
    two minutes.
    (6) After the time had elapsed the potato cube was removed from the water bath and placed
    into another test tube filled with 2 ml Hydrogen peroxide. Observations were made and
    recorded.
    (7) The third potato cube was crushed using the mortar and pestle and placed into another
    test tube containing 2 ml Hydrogen peroxide. A glowing splint was held over the mouth
    of this test tube and observations were made and recorded.
    (8) Using the 10 ml syringe, 2 ml of Hydrogen peroxide was placed into a test tube followed
    by 1 ml of Hydrochloric acid. The last potato cube was then added to this test tube and
    observations were made and recorded.

    RESULTS:
    TABLE 1 SHOWING THE ACTION OF THE ENZYME CATALASE ON HYDROGEN
    PEROXIDE.
    Effect of whole Effect of boiled Effect of Effect of whole
    tissue tissue crushed tissue tissue with acid

    Observations

    DISCUSSION:

    ° What is an Enzyme?
    - reactions occurring in living organisms without being changed themselves.

    ° Why are Enzymes important to living organisms?

    ° Discuss the factors that affect the rate of enzyme activity

    ° What is Catalase and where is it found?

    ° Describe and compare the amount of bubbles formed when the enzyme was exposed to
    different conditions.

    ° Discuss why the activity occurred the way it did under each condition.

    ° Compare your results with what was expected to occur in your theory (factors that affect
    enzyme activity)

    ° Why did the glowing splint relight?

    ° How could the experiment be done better?

    PRECAUTIONS:
    ° Gloves were used when handling the Hydrogen Peroxide and Hydrochloric acid as both can
    cause irritations of the skin.

    SOURCES OF ERROR:
    ° Hydrogen Peroxide decomposes when exposed to light (it is usually kept in a dark bottle).
    Some decomposition may have occurred when the H202 was placed into the test tubes.

    ° The different potato cubes may have contained varying concentrations of the enzyme Catalase.

    CONCLUSION:
    From the experiment it can be concluded that the action of the enzyme Catalase is affected by
    Temperature, Surface Area and pH.

    REFLECTION:
    ° Discuss the importance of maintaining a constant temperature and pH in the body.
    ° Discuss the importance of chewing in digestion.

    Skill: AI
    General Criteria Specific Criteria Marks
    Allotted
    Background ° Description of what is an Enzyme 1
    °How different factors affect the rate
    of enzyme action 1
    Trends ° Description of amount of bubbles 1
    °Comparisons made with different
    factors that affect rate of enzyme 1
    action

    Results & Hypothesis ° Explanation of the effects of 2


    temperature, surface area and pH on
    rate of reaction
    ° Compare actual results with 1
    background theory
    Conclusion ° Well defined concluding statement 1
    Limitations, Precautions and Sources of ° Identification of at least 2 2
    Error
    Total 10

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