Reference Lab
Reference Lab
Reference Lab
POWERCHART
ABO + RH(D) BLOOD TYPING
NAME
MERCY TEST NAME ABO GROUP/RH TYPE MERCY LAB CODE ABRX
Includes: Includes ABO group and Rh type. ABO and Rh are not ordered separately.
Included In: Type & Screen, Crossmatch, Prenatal Profile, Cord Blood Routine, and RHIG Evaluation.
Specimen: o Preferred in house: One 6 ml pink (EDTA) top tube.
o Preferred reference lab; One 6 ml pink (EDTA) top tube.
o Also acceptable: purple top (EDTA) whole blood in original tube.
Stability: 3 days refrigerated.
Performed: Within 8 hours of receipt. Available stat.
Method: Serological
CPT Code: o ABO+ 86900
RH+ 86901
TEST NAME ABG (ARTERIAL BLOOD GASES) Done by Cardio-Vascular & Pulmonary. For capillary
gases see
Specimens may be stored for up to 8 hours at 20-25 °C or stored frozen for up to 45 days at
Stability
-20°C
Method The Atellica CH Acetaminophen (Acet) assay is based on the conversion of acetaminophen by
Description: acyl amidohydrolase to produce p-aminophenol is then converted to a colored complex
produced by reacting with 8-hydroxyquinoline-5-sulfonic acid.
Specimen: 2 ml serum from a no additive serum tube or a Serum Separator Tube (SST).
Stability: 28 days refrigerated: 72 hours ambient, frozen OK
Processing: Send refrigerated to Mayo. Mayo order code (ARBI).
Performed: Report available in 3 to 6 days.
Reference value: Included in report
Method: Radioimmunoassay (RIA)
CPT Code: 86041
Stability: N/A
Comment: AG Ratio is a calculation and not orderable by itself. Included in CMPL, GHP, HFPL, NUTP,
DPNL
Method
Calculation
Description:
Specimens may be stored for up to 3 days at 2-8°C or stored frozen for up to 30 days at -
Stability:
20°C.
Method The Atellica CH Albumin BCP (AlbP) assay is an adaptation of the bromocresol purple (BCP)
Description: dye-binding method
MERCY TEST NAME ALCOHOL ETHYL BLD MERCY LAB CODE ALCO
Specimens must be stored in capped or sealed containers. Specimens may be stored for up
Stability: to 2 days at room temperature. Serum may be stored for up to 2 weeks at 2-8°C or
indefinitely when stored frozen at -20oC or indefinitely when stored frozen at -20oC or below.
Comment: Laboratory personnel will not draw legal alcohols, if a legal issue is involved, refer to Nursing
Supervisor.
POWERCHART ALDOLASE
NAME
Specimen: o 1.2 ml serum from a Serum Separator Tube (SST) or a no additive serum tube. 0.6
ml minimum.
o Collect at 0800. 8 a.m. draw time (after the patient is active for 2 hours) is
recommended; preferably no later than 10 a.m. This is the preferred time because
of normal range but will accept other times.
o Specify specimen source as multiple specimens may be collected during a
procedure
Processing: Send frozen to Mayo. Refrigerated and ambient acceptable. Mayo order code (ALDS).
Performed: Monday - Friday
Reference value: Included with report
Specimens may be stored for up to 8 hours at 25°C or for up to 7 days at 2–8°C or stored
Stability:
frozen for up to 6 months at -20°C or colder.
Method The Atellica CH Alkaline Phosphatase, concentrated (ALP_2c) assay is based on the primary
Description: reference procedure for the measurement of catalytic activity of alkaline phosphatase at
37°C as described by the International Federation of Clinical Chemistry (IFCC).
MERCY TEST NAME BONE ALK PHOS* MERCY LAB CODE BALP
Specimen: 0.6 ml serum from a Serum Separator Tube (SST) or a no additive serum tube. 0.5 ml
minimum
Cause for Hemolyzed and/or Lipemic specimens are unacceptable.
Rejection:
Processing: Send refrigerated to Mayo. Frozen acceptable.
Mayo test order code BAP.
Performed: 1 - 3 day(s). Test performed Monday through Friday 5 a.m.-midnight, Saturday 6 a.m.- 6 p.m.
Reference value: Included with test results.
Method: Immunoenzymatic Assay
CPT Code: 84080 Bone Alkaline Phosphatase
MERCY TEST NAME ALK PHOSPH ISO MERCY LAB CODE ALKI
Comment: The multiple allergen screen is a very sensitive first-order test for allergic disease.
Also includes Immunoglobulin E testing.
Send 1 miscellaneous chemistry order for each multiple allergen screen requested, putting
allergen screen name in comment field.
(Example: you would need 1 order for major molds allergen screen, another order for dairy
allergen screen, etc...)
Please contact the Lab if you need help to determine which allergen screen is appropriate or
need to know what specific allergens are being tested in a particular allergen screen.
Processing: o See Mayo catalog or Special Helps section of Lab Test Index for special instructions
for specific Mayo ordering numbers for each allergen group.
o Mayo requests that each allergen screen is to have its own Mayo number.
o Send a separate vial of serum for each allergen screen ordered.
o Send refrigerated to Mayo.
Comment: This test is useful principally to confirm the Allergen specificity in patients with clinically
documented allergic disease.
Please note:
This test is for a specific allergen requested. (Such as alfalfa grass or corn grass or birch tree,
etc.) If the doctor is looking to see if the patient is allergic to grasses or trees, etc., then an
allergen multiple screens should be ordered. Call Lab for help in ordering.
Send 1 order per specific allergen requested. Designate specific allergen to be tested. Refer
to Special Helps Section of the Lab Test Index for a complete list of allergens available
for testing and their corresponding test order codes.
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: o See Mayo catalog, special instructions for specific Mayo ordering numbers for each
allergen.
o Send refrigerated to Mayo.
o Mercy Lab staff will order on Mayo Access.
Performed: 1 day. Test set up Monday through Friday.
Reference value: Included with report.
Method: Fluorescence Enzyme Immunoassay (FEIA)
CPT Code: 86003
Comment: This profile includes, egg whites, milk, cod fish, wheat, peanut, and soybean.
Specimen: 1.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Minimum volume calculation: (0.05ul x # of allergens) + 0.25 ul
Processing: Send refrigerated to Mayo.
Mayo test order code (PR207).
Performed: 1-5 days. Test set up Monday through Friday.
Reference value: Included with report.
Method: Fluorescence Enzyme Immunoassay (FEIA)
CPT Code: 86003 x6
Specimen: 3.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Minimum volume calculation: (0.05ul x # of allergens) + 0.25 ul
Processing: Send refrigerated to Mayo.
Mayo test order code (PR350).
Performed: 1-5 days. Test set up Monday through Friday.
Reference value: Included with report.
Method: Fluorescence Enzyme Immunoassay (FEIA)
CPT Code: 86003 x12
Comment: This profile includes cat epithelium, dog epithelium, house dust mites DP, house dust mites
DF, Alternaria tenuis, giant ragweed, short ragweed, timothy grass, silver birch, mugwort,
epicoccum purpurascens, corn pollen, box elder/maple, oak and Johnson grass.
Specimen: 4.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Minimum volume calculation: (0.05ul x # of allergens) + 0.25 ul
Processing: Send refrigerated to Mayo.
Mayo test order code (PR894)
Performed: 1-5 days. Test set up Monday through Friday.
Reference value: Included with report.
Method: Fluorescence Enzyme Immunoassay (FEIA)
CPT Code: 86003 x15
TEST NAME ALLERGEN PROFILE 138 MERCY LAB CODE PR138
Comment: This profile includes English Plantain, Epicoccum purpurascens, Firebush (Kochia),
Helminthosporium halodes, Lamb's Quarter, Mugwort
Specimen: 4.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Minimum volume calculation: (0.05ul x # of allergens) + 0.25 ul
Processing: Send refrigerated to Mayo.
Mayo test order code (PR138)
MERCY TEST NAME SINGLE MARKER AFP* MERCY LAB CODE SMAFP
Specimen: 1 ml serum from a Serum Separator Tube (SST) (Preferred) or no additive serum tube also
acceptable. Spin down immediately
Comment: o To be run between the 15th and 22nd gestational week.
o The Mayo information form must be completed and sent with the
patient/specimen.
o Forms available from the Laboratory or from the intranet LTI - Special Helps
Section. AFP Form.
o Do not collect specimen after amniocentesis as this could affect results.
Processing: o 1.0 ml serum refrigerated to Mayo. Frozen and ambient also acceptable.
o Mayo test order code (MAFP1).
o Must send the Mayo AFP form with the specimen.
Performed: 1-3 days Monday through Friday
Reference value: Included with test results
Methods: Two-Site Immunoenzymatic (Sandwich) Assay
MERCY TEST QUAD SCRN 2ND TRI* MERCY LAB CODE QUADM
NAME
Specimen: 1 ml serum from a Serum Separator Tube (SST) (Preferred) or no additive serum tube. Spin
down immediately.
Comment: o Do not collect specimen after amniocentesis as this could affect results.
o To be run between the 15th and 22nd gestational week.
o Assessments for trisomy 21 (Down Syndrome) and Trisomy 18 (Edwards syndrome)
are only available between 14 weeks and 22 weeks.
oThe Mayo information form must be completed and sent with the
patient/specimen. Forms available from the Laboratory or from the intranet LTI -
Special Helps Section. AFP Form.
o Initial or repeat testing is determined in the laboratory at the time of report and will
be reported accordingly. To be considered a repeat test for the patient, the testing
must be within the same pregnancy and trimester, with interpretable results for the
same test, and both tests are performed at Mayo Clinic.
Processing: o 1.0 ml serum refrigerated (Preferred) to Mayo. Frozen and ambient also
acceptable.
o Mayo test order code (QUAD1).
o Must send the Mayo AFP form with the specimen.
Performed: 1-4 days, Monday through Friday
Reference value: Included with test results
Method: Immunoenzymatic Assay
Comment: Keep tubes capped at all times. Do not use specimens that have been stored at room
temperature for longer than 8 hours.
Reference
0.0-9.0 ng/mL
Range:
Method The Atellica IM AFP assay is a 2-site sandwich immunoassay using direct chemiluminescent
Description: technology
Specimen: 1 ml serum from a Serum Separator Tube (SST) or a no additive serum tube
Processing: Send refrigerated to Mayo. Frozen or ambient acceptable.
Mayo test order code AAT
Note: **Included in Alpha-1 Antitrypsin Phenotype testing Mercy Lab Code ALAN, do not order both.
Performed: Test set up Monday through Saturday.
Reference value: Included with test results.
Method: Rate Nephelometry
CPT Code: 82103
MERCY TEST NAME ALPH1 ANTITRYP PHEN* MERCY LAB CODE ALAN
Specimen: 1.25 ml serum a no additive serum tube. Serum from a SST is also acceptable.
Processing: Send refrigerated to Mayo. Frozen or ambient acceptable.
Mayo test order code A1APP
Note: **Do not order ALPHA - Alpha-1 Antitrypsin in addition to this order, it would be a duplicate.
Alpha-1 Antitrypsin is included in this ALAN - Alpha-1 Antitrypsin Phenotype testing.
Performed: 2-6 Days; Monday through Friday.
Reference value: Included with test results.
Method: Isoelectric Focusing: Nephelometry
CPT Code: 82103 - Alpha-1 Antitrypsin
82104 - Alpha-1 Antitrypsin Phenotype
POWERCHART ALT/SGPT
NAME
Separated specimens may be stored for up to 7 days at 2–8°C or stored frozen for up to 30
Stability:
days at -20°C or colder.
Comment: Avoid repeating freezing and thawing. Venipuncture should occur prior to Sulfasalazine
administration due to the potential for falsely depressed results.
Method The Atellica CH Alanine Aminotransferase (ALT) assay is based on a reaction initiated by the
Description: addition of α-Ketoglutarate as a second reagent. The concentration of reduced nicotinamide
adenine dinucleotide (NADH) is measured.
Specimen: o 1.2 ml serum from a navy blue top no additive trace metal tube. 0.3 ml minimum.
o Always draw this tube first if multiple tubes are being drawn.
o Use alcohol, not iodine to cleanse venipuncture site.
o If a syringe is needed, use only Mayo blue-labeled metal-free polypropylene
syringe.
Cause for
The use of other tubes is unacceptable.
rejection:
Processing: o Allow to clot well.
o After centrifugation, pour (DO NOT use transfer pipette or wooden sticks) serum
into blue-labeled 5ml Mayo metal-free, screw-capped polypropylene vial.
o Send refrigerated to Mayo. Ambient and frozen also acceptable.
o Mayo test order code AL
o If specimen will be stored more than 48 hours, send frozen.
Performed: 1-5 days. Wednesday, Friday, 5 p.m.
Reference value: Included with report.
Specimen: o 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
o Specimen should be drawn 30 to 60 minutes after last dose. Spin specimen down
within 2 hours of draw. Send serum specimen in a plastic vial
Mayo Test
Mayo order code (PAMIK).
number/Code:
RL Comment: Send specimen refrigerated to Mercy Lab. Frozen and Ambient specimens are acceptable.
Specimen: o 0.5 ml of serum from a Serum Separator Tube (SST) or no additive serum tube.
o Spin specimen down within 2 hours of draw. Send serum specimen in a plastic vial.
Mayo Test
Mayo order code (RAMIK).
number/Code:
RL Comment: Send specimen refrigerated to Mercy Lab. Frozen and ambient specimens are acceptable.
Specimen: o 0.5 ml of serum from a Serum Separator Tube (SST) or no additive serum tube.
o Draw blood immediately before next scheduled dose. Spin specimen down within
2 hours of draw. Send serum specimen in a plastic vial.
Mayo Test
Mayo order code (TAMIK).
number/Code:
RL Comment: Send specimen refrigerated to Mercy Lab. Frozen and ambient specimens are acceptable.
Specimen: 0.5 ml plasma from a lithium heparin green top tube. The tube should be completely filled
and placed on ice and centrifuged without delay.
Comment: Serum samples will be rejected. Hemolyzed samples will be rejected. Lipemic or icteric
samples my yield invalid results. Venipuncture should occur prior to Sulphapyridine
administration due to the potential for falsely depressed results.
Stability:
Separated specimens may be stored for up to 2 hours at 2–8°C. The tube should be
completely filled, stored tightly capped on ice and centrifuged without delay.
Samples should be analyzed within 30 minutes of centrifugation. Concentrations may more
than double in plasma when stored at room temperature for 6 hours.
Method The Atellica CH Amm assay is an enzymatic assay that uses glutamate dehydrogenase
Description: (GLDH) and a stabilized NADPH analog. Ammonia reacts with α-ketoglutarate and reduced
cofactor to form L-glutamate and the cofactor. The reaction is catalyzed by glutamate
dehydrogenase.
POWERCHART AMYLASE
NAME
Stability:
Separated serum specimens may be stored for up to 8 days at room temperature or up to
31 days at 2–8°C6 or stored frozen for at least 1 year at -20°C.
Stability: Specimens may be stored for up to 8 days at room temperature or for up to 31 days at 2–
8°C6 or stored frozen for at least 1 year at -20°C.
Method The Atellica CH AMY_2 assay uses ethylidene blocked p-nitrophenyl- maltoheptaoside as
Description: substrate. The indicator enzyme α-glucosidase, used to release p-nitrophenol (PNP), is also
employed in the assay. The terminal glucose of the substrate is chemically blocked,
preventing cleavage by the indicator enzymes.
Specimen: 1 mL serum from a Serum Separator Tube (SST)(Preferred) or no additive serum tube.
Serum gel tubes should be centrifuge within 2 hours of collection. Red-top tubes should be
centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.
Processing: Send refrigerated (preferred) to Mayo. Ambient or frozen is also acceptable. Mayo order
code (AMISO).
Performed:
Within 8 hours of receipt.
Reference value: 120-648 U/24 Hours
Method: Enzymatic Rate
CPT Code: 82150
MERCY TEST NAME AMYLASE QUANTITATIVE URINE MERCY LAB CODE XAMY
Specimen: 5 ml urine
Stability: Urine amylase is unstable in acidic urine. Adjust urine to a pH ≥ 7 before storage. Adjusted
urine specimens may be stored for up to 10 days at room temperature6 or for up to 31 days
at 2–8°C.
Method: The Atellica CH AMY_2 assay uses ethylidene blocked p-nitrophenyl- maltoheptaoside as
substrate. The indicator enzyme α-glucosidase, used to release p-nitrophenol (PNP), is also
employed in the assay. The terminal glucose of the substrate is chemically blocked,
preventing cleavage by the indicator enzymes.
Specimen: 5 ml urine
Stability: Adjusted urine specimens may be stored for up to 10 days at room temperature6 or for up
to 31 days at 2–8°C.
Comment: Urine amylase is unstable in acidic urine. Adjust urine to a pH ≥ 7 before storage.
Swab specimens: Embed swab deeply into Port-A-Cul Cary Blair tube and cap tightly. Two
swabs from the same specimen site should be submitted in one transport tube.
Fluid or pus aspirates: Inject specimen into Port-A-Cul vials. DO NOT inject air into vial.
Reference values: No anaerobes isolated (applies to normally sterile body sites). Varies with site of collection.
Patient
Patient must be fasting.
preparation:
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Cause for Hemolyzed specimens are unacceptable.
rejection: The use of ACE-inhibiting antihypertensive drugs will cause decreased values.
Processing: Send refrigerated to Mayo.
Mayo test code (ACE).
Performed: 1-3 days. Test set up Monday through Saturday.
Reference Value: Included with report
Method: Spectrophotometry
CPT Code: 82164
Stability: Specimens may be stored for up to 7 days at 2–8°C or stored frozen for up to 30 days at -
20°C.
Comment: Anion gap is a calculation and is not orderable by itself. Included in METB, CMPL, LYTE, GHP,
RPNL, NUTP, TPNL
Method
Calculation
Description:
Regional Lab Clients: Order Antibody Screen. An antibody identification will be ordered and
charged only if screen is positive. Please see Special Helps Section for further information.
Cause for
Serum
rejection:
Performed: Within 24 hours of receipt.
Method: Serological
CPT Code: 86870
POWERCHART ANTIBODY SCREEN
NAME
Specimen: 1-6 ml pink top tube. All tubes must be labeled with the patient's name, date, and medical
record number. Refrigerate.
Cause for
SST tube is unacceptable.
rejection:
Comment: o Blood Bank will order and charge for an Antibody Screen, and if positive, order and
charge for an Antibody Identification if one has not been done within the previous
72 hours.
o Titer will be performed by LifeServe Blood Center if Titer is indicated.
Mercy Medical Center-North Iowa Blood Bank Staff will order and perform an antibody
screen using both solid phase and LIS method. If solid phase antibody screen is positive,
antibody ID will be performed at MMC-NI. After the initial testing is performed at MMC-NI,
the specimen will be forwarded to LifeServe Blood Center if titer is indicated.
MERCY TEST NAME AB EXTRCT NUCLR AG* MERCY LAB CODE ENAE
Specimen: 0.5 ml serum from a no additive serum tube or a Serum Separator Tube (SST) is acceptable.
Processing: Send refrigerated to Mayo. Frozen is acceptable. Mayo order code (ENAE).
Performed: 1-2 days. Monday through Saturday; 4 P.M.
Reference Value: Included in report
Method: Multiplex Flow Immunoassay
CPT Code: 86235 x6
TEST NAME ANTIMULLERIAN HORMONE
Patient For 12 hours before specimen collection do not take multivitamins or dietary supplements
Preparation: containing biotin (Vitamin B7), which is commonly found in hair, skin, and nail supplements
and multivitamins
Specimen: 1.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: Send refrigerated to Mayo. Ambient and Frozen are also acceptable.
Mayo order code (AMH1).
Performed: Report available in 1-3 days.
Reference value: Included in report.
Method: Electro chemiluminescent Immunoassay (ECLIA)
CPT Code: 82166
Specimen: 1.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: Send refrigerated to Mayo. Ambient and Frozen are also acceptable.
Mayo order code (ANA2).
Performed: 1 day. Testing performed Monday through Saturday.
Reference value: Included in report.
Method: Enzyme-Linked Immunosorbent Assay (ELISA)
CPT Code: 86038
TEST NAME ANTINUCLEAR AB HEP-2 SUBSTRATE
MERCY TEST ANA HEP2 SUBSTRATE* (ANA titer) MERCY LAB CODE ANAH2
NAME
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: Send refrigerated to Mayo. Frozen is also acceptable.
Mayo order code (NAIFA).
Performed: 1 day. Testing performed Monday through Saturday
Reference value: Included with results
Method: Indirect Immunofluorescence
CPT Code: 86039
Specimen: One 6 ml pink top tube or a purple top tube. Red Cells are needed for testing.
Comment: o Order Miscellaneous Immunohematology. Enter specific antigen to be tested in
comment.
o Reference Lab Clients: Mark OTHER on requisition form. Write antigen typing and
the specific antigen(s) to be tested.
Processing: Centrifuge. Refrigerate specimen.
Performed: Within 8 hours of receipt.
Method: Serological
CPT Code: 86905
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo order code (SM)
Performed: 1-2 days. Monday through Saturday; 4 p.m.
Reference value: Included in report.
Method: Multiplex Flow Immunoassay
CPT Code: 86235
MERCY TEST NAME ANTI SMOOTH MUS AB* MERCY LAB CODE SMAB
Specimen: 0.8 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: Send refrigerated to Mayo. Ambient and frozen also acceptable. Mayo order code (SMAS).
Performed: 2 days. Test set up Monday through Saturday.
Reference value: Included in report.
Method: Indirect Immunofluorescence
CPT Code: 86015 Screen
86015 If Appropriate
POWERCHART ANTITHROMBIN III ACTIVITY
NAME
Processing: Spin down, remove plasma, and spin plasma again and place 1.0 mL platelet poor plasma in
plastic aliquot vial. Freeze immediately.
Processing Spin down, remove plasma, and spin plasma again. Remove plasma and place 1.0 mL
Instructions: platelet poor plasma in plastic aliquot vial. Freeze specimens immediately at < or = -40
degrees C, if possible. Label specimens as plasma.
Shipping
Send plasma frozen. Mayo order code (ATTI).
Instructions:
Specimens are stable when separated for up to 2 days at 2–8°C or stored frozen for up to 6
Stability:
months at -20°C or colder.
Method The Atellica CH ASO_2 assay measures ASO antibodies in serum or plasma by a latex-
Description: enhanced immunoturbidimetric method.
Specimen: 1.5 ml serum in am Serum Separator Tube (SST) **DO NOT ALIQUOT**
Processing: DO NOT ALIQUOT Send serum in original tube, send refrigerated to Mayo. Mayo test order
code: ASPAG
Performed: 1-3 days. Monday through Friday 9-4, Sunday 8
Reference Value: Included in Report
Method: Enzyme Immunoassay (EIA)
CPT Code: 87305
POWERCHART AST/SGOT
NAME
Separated specimens may be stored for up to 3 days at 20–25°C or for up to 7 days at 2–8°C
Stability:
or stored frozen for up to 30 days at -20°C or colder.
Method The concentration of reduced nicotinamide adenine dinucleotide (NADH) is measured, and
Description: the rate of absorbance decrease is proportional to the AST activity.
MERCY TEST NAME AUTO IMM LIVER PNL* MERCY LAB CODE ALDP
Specimen: 1.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Comment: Duplicate testing if ordered with MTAB (Mayo AMA) or SMAB (Mayo SMAS)
Method: AMA: Enzyme Immunoassay (EIA) NAIFA, SMAS, SMAT: Indirect Immunofluorescence
Specimen: Submit each organism to be identified on a separate plate. Colonies should be well
isolated.
TEST NAME BARR BODY SMEAR See: Cytology Section Barr Body Smear
Specimen: 0.5 mL serum from a Serum Separator tube (SST) or no additive serum tube
Stability: 30 days refrigerated (preferred), 30 days frozen.
Performed: 1-3 days, Monday through Saturday; 9 a.m.
Reference value: Included with test results.
Method: Immunofluorescence Assay (IFA) technique using antigen substrate slides consisting of 2
separates cell cultures infected with Bartonella henselae or Bartonella Quintana.
CPT Code: 86611 x 4
POWERCHART (BMP) BASIC METABOLIC PANEL
NAME
Stability: Specimen may be stored for up to 7 days at 2-8 °C or frozen for up to 30 days at -20°C.
Comment: Includes Anion Gap, BUN, BUN/Creatinine Ratio, Calcium, Chloride, CO2, Creatinine, eGFR,
Potassium, Sodium, Glucose
MERCY TEST NAME BCRAB MRNA DETECT (BCR ABL P210 MRNA MERCY LAB CODE BCRAB
DETECTION)
Submit only 1 of the following:
Specimen: o
o 10 mL whole blood from lavender top tube (EDTA)
o 3 mL bone marrow from lavender top tube (EDTA)
Send to Mayo lab refrigerated. Specify on requisition specimen type. Order Mayo test (BCRAB).
Process:
Specimen must arrive at Mayo within 72 hours of collection.
Performed: 3-6 days. Test set up Monday through Friday.
Reference value: Included in report.
Method: Quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) using GeneXpert
CPT Code: 81206
MERCY TEST NAME BETA 2 GP1 AB IGA* MERCY LAB CODE AB2GP
Specimen: 0.5 ml serum from a Serum Separator tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo, Frozen is acceptable. Mayo order (AB2GP).
Performed: 3 - 5 days, Monday, Wednesday, Friday
Reference value: Included in report.
Method: Enzyme-Linked immunosorbent Assay (ELISA)
CPT Code: 86146
Specimen: 0.5 ml serum from a Serum Separator tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo, Frozen is acceptable. Mayo order (GB2GP).
Performed: Monday through Saturday; 8 a. m.
Reference value: Included in report.
Method: Enzyme-Linked immunosorbent Assay (ELISA)
CPT Code: 86146
MERCY TEST NAME BETA2 GPI IGG IGM* MERCY LAB CODE B2GMG
Specimen: 0.5 ml serum from a Serum Separator tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo, Frozen is acceptable. Mayo order code (B2GMG).
Performed: 1-6 days. Monday through Saturday; 8 a. m.
Reference value: Included in report.
Method: Enzyme-Linked immunosorbent Assay (ELISA)
CPT Code: 86146 x2
Specimen: 1.0 ml serum from a Serum Separator tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo, ambient or frozen. Mayo order code (B2M).
Performed: Monday - Saturday, 3 PM
Reference value: Included in report.
Method: Nephelometry
CPT Code: 82232
Order General Miscellaneous Lab, specify in comment: Mayo order code (BETA2) Beta-2
Transferrin, and source of specimen.
0.5 ml body fluid such as ear or nasal fluid, or other fluid. Indicate specimen type.
Specimen:
NOTE: Although results may be obtainable on smaller specimens (perhaps as little as 0.05ml,
depending on the protein concentrations and percentage of spinal fluid in the specimen),
Reliable results are best obtained with an adequate specimen volume.
MERCY TEST NAME Beta Hydroxybutyrate Level MERCY LAB CODE BHOB
MERCY TEST NAME BILE ACIDS TOTAL* MERCY LAB CODE BILEA
Specimen: 0.5 mL serum from a Serum Separator tube (SST) or no additive serum tube
*Patient must be fasting 8 hours minimum. Do not order on patients receiving bile
acid therapy.
Processing: Serum gel tubes should be centrifuged within 2 hours of collection. Red top tubes should be
centrifuged and aliquoted within 2 hours of collection. Send to
Mayo refrigerated. Frozen is acceptable. Mayo code (BILEA)
Stability: Specimen should be stored at 4°C and analyzed within 5 days. Specimens may be stored
frozen for up to 3 months at -70°C with no light exposure.
Comment: Bilirubin is extremely photosensitive. Care should be taken to protect sample from both
daylight and fluorescent light to avoid photodegradation.
Method: The Atellica CH Direct Bilirubin_2 (DBIL_2) assay is based on a chemical oxidation method
using vanadate as an oxidizing agent.
Stability: Specimen should be stored at 4°C and analyzed within 5 days. Specimens may be stored
frozen for up to 3 months at -70°C with no light exposure.
Comment: Bilirubin is extremely photosensitive. Care should be taken to protect sample from both
daylight and fluorescent light to avoid photodegradation. Panel includes total bilirubin,
direct bilirubin, and indirect bilirubin.
Performed: Within 8 hours of receipt. Available stat.
Method: The Atellica CH Bilirubin assay is based on a chemical oxidation method using vanadate as
an oxidizing agent.
Stability: Specimen should be stored at 4°C and analyzed within 5 days. Specimens may be stored
frozen for up to 3 months at -70°C with no light exposure.
Comment: Bilirubin is extremely photosensitive. Care should be taken to protect sample from both
daylight and fluorescent light to avoid photodegradation. Panel includes total bilirubin,
direct bilirubin, and indirect bilirubin.
Method: The Atellica CH Total Bilirubin_2 (TBil_2) assay is based on a chemical oxidation method
using vanadate as an oxidizing agent.
MERCY TEST NAME BK VIRUS PCR QUANT MERCY LAB CODE QBK
Specimen: 1.5 mL plasma from EDTA tube. Centrifuge within 2 hours of collection and removed plasma.
Stability: 84 days frozen (preferred), 6 days refrigerated
Specimen: 1 mL serum from a Serum Separator Tube (SST) (Preferred). Serum from a no additive
serum tube is also acceptable. Centrifuge within 2 hours of collection. For red-top tubes,
immediately aliquot serum into a plastic vial. For serum gel tubes, aliquot serum into a
plastic vial within 24 hours of collection.
Processing: Send refrigerated to Mayo. Frozen is also acceptable. Mayo order code (BLAST).
Draw enough citrated whole blood to spin down and aliquot 5.0 mL platelet poor plasma in
5 plastic aliquot vials. Spin down, remove plasma,
and spin plasma again. Remove plasma and place in plastic aliquot vials. Freeze immediately
at < or = -40 degrees C, if possible. Label
Processing:
specimens as plasma.
Shipping
Send plasma frozen. Mayo code (ALBLD).
Instructions:
Coagulation factor VIII assay – 85240, von Willebrand factor antigen – 85246
Factor IX – 85250, Clot solubility factor XIII – 85291
D-dimer – 85379, Fibrinogen – 85384
von Willebrand factor activity – 85397, PT – 85610
Thrombin Time – 85670, APTT - 85730
If indicated, the following reflex tests will be ordered by Mayo at an additional cost:
Factor II - 85210
Factor V - 85220
CPT Code:
Factor VII - 85230
Ristocetin cofactor - 85245
Von Willebrand factor multitimer - 85247
Factor X - 85260
Factor XI - 85270
Factor XII – 85280, Plasminogen Activity - 85420
Bethesda units – 85335, PAI-1 Ag - 85415
Platelet neutralization for lupus inhibitor – 85597, Chromogenic FVIII,P-85130
Reptilase time – 85635, Chromogenic IX,P - 85130
APTT mix 1:1 – 85732, Antithrombin Antigen -85301
Factor V Inhibitor screen – 85335, Antithrombin Activity - 85300
Factor VIII Inhibitor screen – 85335, PT-Fribrinogen - 85385
Factor IX Inhibitor screen – 85335, Soluble Fibrin Monomer - 85366
Alpha-2 Plasmin Inhibitor - 85410, HEX LA,P - 85598
PTT mix 1:1 – 85611, DRVVT - 85613
DRVVT mix – 85613, DRVVT - 85613
Specimen: The specimen submitted should be the positive blood culture bottle.
o This order is to be user ONLY by Reference Lab clients that have a positive blood
culture, at their facility, which requires further workup at Mercy Lab.
o Do not submit any media plates if this order is used. The actual blood culture
bottle should be submitted.
Comment:
o Fax the white Reference Lab Examination sheet that indicates specifics about the
specimen. The Mercy Microbiology fax number is found at the top of the form.
o Please place the Blood Culture ID labels on the neck of the bottle to alert Mercy
staff of the ID only.
Performed: Daily.
Method: Routine culture method.
CPT Code: 87040
Order: Order Miscellaneous microbiology. Specify Mayo code: CTBBL MYCOBACTERIAL CULTURE,
BLOOD in comment
Specimen: Draw 10 ml heparinized (green top tubes) whole blood using aseptic technique. Invert tubes
to mix.
Processing: Specimens need to be processed immediately upon being drawn. SPECIMENS MUST ARRIVE
AT MAYO LAB WITHIN 72 HOURS OF BEING DRAWN
RL Client o Write MYCOBACTERIAL CULTURE, BLOOD (Mayo order code CTBBL) on order form.
Comments: o Send the specimen to Mayo Lab within 72 hours of drawing the specimen.
o Send the heparinized green top vacutainer tubes to Mercy lab at room
temperature or refrigerated.
Performed: Monday through Sunday; Continuously
Specimen: Patients 6 yrs of age and older: 10 ml whole blood drawn into Isolator 10 tube. Short
samples decrease the already low number of organisms.
Patients 5 years of age and under: 1.5 ml whole blood drawn into pediatric Isolator tube.
TEST NAME BLOOD GAS ANALYSIS Arterial Blood Gases are collected and performed by
Cardio-Vascular & Pulmonary. Capillary Blood
Gases see: COLLECTION CAPILLARY CHARGE BLOOD
GASES
MERCY TEST NAME BLOOD PARASITES (MALARIA SMEAR) MERCY LAB CODE MAL
Specimen: Non-anticoagulated venous blood or peripheral blood from finger or earlobe preferred.
Prepare 3 thick and 3 thin smears on separate slides. EDTA blood can be used and should
also be sent with the slides.
THICK SMEARS: Place a drop of blood on a slide. Using the corner of a clean slide spread the
blood in a circle about 20 times until the size of a quarter by literally scratching the blood
onto the carries slide. Prepare at least 2 slides. This technique allows the blood to dry quickly
and adhere well to the slide. If proper thickness is achieved, ordinary print should barely be
visible through the wet center.
Allow both thick and thin films to air dry without heating. The thick smear must dry 8-10
hours before staining.
Comment: Collection available stat. Collection time is determined by the fever pattern. Consult the
Microbiology Department. If the smear is considered positive, it will be referred to Mayo for
identification and will incur extra charges.
Performed: Within 24-72 hours of receipt.
Reference value: No malaria or blood parasites seen.
Method: Microscopy, Giemsa/Wright-stained smears.
CPT Code: 87207
MERCY TEST NAME BLOOD PATCH COLL MERCY LAB CODE PTCH
The floors will call the lab and ask that a phlebotomist come to the patient’s room to assist
the Anesthesiologist in the blood drawing for the epidural blood patch.
Comment:
o The floor will place an order on the Powerchart system the test name is Blood
Patch Collection.
o This is a “sterile procedure”. Anesthesia staff will explain the procedure to the
patient while the Phlebotomist is putting on the gown, gloves, and mask.
o With the patient lying on their side, the Phlebotomist will stand in front of the
patient. The patient’s arm is to be extended toward you.
o As the doctor is preparing the site on the patients back, the Phlebotomist will
prepare the arm site to perform the venipuncture, using chloraprep to cleanse,
allow to air dry. The doctor provides a sterile 20cc syringe. The Phlebotomist will
provide a 20g sterile needle or a 21g sterile butterfly.
o When the doctor is in the epidural cavity of the spine, they will say, “Okay, draw”.
The Phlebotomist will quickly draw 20 cc of blood from the vein, keeping a sterile
field, and hand the syringe to the doctor.
o The doctor will inject the blood into the spinal cavity of the patient.
o Place a gauze and paper tape over the venipuncture site and return to the lab.
o Use function CLVS to verify the collect time in Sunquest. For the phlebotomy
workload code, use PA, comma then enter the length of time the procedure took.
DO NOT add a collect charge.
o In function ME, use worksheet PHL, type in PATC and accept.
Stability: If plasma samples are not tested within 24 hours, store samples in plastic tubes and freeze
at ≤ -20°C. Do not store in a frost-free freezer.
Samples may undergo up to 4 freeze-thaw cycles without degradation.
Samples are stable for up to 9 months when stored at ≤ -20°C.
Mix samples thoroughly after thawing and store at 2-8°C until use.
Samples should be tested within 8 hours after thawing.
Comment: After centrifugation, store separated plasma samples at 2-8°C until testing.
For optimal recovery of BNP values, it is suggested that plasma samples be tested within 24
hours. The average percentage of BNP recovery in EDTA plasma after 24-hour-storage at 2-
8°C was 91%. It is recommended not to store EDTA-plasma at room temperature.
Method The Atellica IM BNP assay is a fully automated 2-site sandwich immunoassay using direct
Description: chemiluminescent technology which uses constant amounts of 2 monoclonal antibodies.
MERCY TEST NAME BODY FLD CLT/GS MERCY LAB CODE FLDC
Specimen: Collect aseptically by needle aspiration or surgical procedure. Submit all specimens in a
sterile syringe with the needle discarded or sterile screw top container or tube.
Specimen stability: All listed specimens are stable <24 hours, room temperature
EXCEPT Pericardial. Pericardial samples are stable <24 hours refrigerated.
o Bone marrow: 1.5 ml placed in a Wampole Isolator tube (available from lab)
o CSF fluid: 1 ml minimum, placed in sterile screw capped tube. Do not refrigerate.
o Joint: 1 ml aspirate
o Pericardial: 1 ml aspirate
o Peritoneal: 1-2 ml aspirate
o Pleural: 5-10 ml aspirate
o Thoracic: 5-10 ml aspirate
Cause for Fluid injected into a CULTURETTTE is unacceptable. A SWAB SPECIMEN IS NOT
rejection: ADEQUATE.
Performed: Gram stain: Within 8 hours of receipt, unless ordered STAT Preliminary reports: Days 1 and 2
Final report: 3 days
Comment: Not available stat. Nursing service must also fill out a yellow Surgical Specimen Slip and
a white bone marrow history form to include patient history and clinical diagnosis.
Available Monday through Friday, 0700-1500. If a bone marrow examination is needed
outside these hours, special arrangements may be made by contacting the Laboratory. Send
Lab a message of all bone marrows scheduled (include date and time). Nursing Service is
to schedule with:
Specimen: Procedure will include collection of the following: 6 smears for Wright's Stain, smear for Iron
Stain, CBC and Cell Morphology, 2 peripheral smears, Bone Marrow Clot and Biopsy. Lab will
order the CBC if one has not been done within the previous 24 hours.
MERCY TEST NAME BREATH ALCOHOL TESTING MERCY LAB CODE BATHW
Performed: Monday - Friday 1630-0800. Performed by Healthworks from 0800-1630. Saturday and
Sunday, available 24 hours.
MERCY TEST NAME BRONCH QAL CLT/GS MERCY LAB CODE BQAL
Order: Specify from which bronchus the specimen is collected when ordering.
Specimen: Minimum of 5 ml of bronchus washings collected through the inner chamber of the
bronchoscope. Submit in a sterile plastic container with a tight-fitting lid.
MERCY TEST NAME BRONCH QNT CLT/GS MERCY LAB CODE BQNT
To be ordered ONLY on Protected Brush Bronch Specimens (PSB). (Other Bronch sources
should be ordered as a QUAL culture).
Specimen: 1 ml protected specimen brushings (PSB) placed in 1 ml normal saline. Submit in a sterile
plastic container with a tight-fitting lid. Quantity of saline added is critical for accurate
quantitation.
Reference value: No growth or Scant Normal flora of the upper respiratory tract.
TEST NAME BUCCAL SMEAR See: Cytology Section Barr Body Smear
Stability: Separated blood urea nitrogen is stable in separated serum or plasma and may be stored
for up to 3-5 days at room temperature or for up to 7 days at 4°C or stored frozen
indefinitely at -20°C.
Comment: Blood samples from some patients with monoclonal gammopathies may produce falsely
elevated results.
Method Urea is hydrolyzed in the presence of water and urease to produce ammonia and carbon
Description: dioxide. The ammonia reacts with 2-oxoglutarate in the presence of glutamate
dehydrogenase and reduced nicotinamide adenine dinucleotide (NADH). The oxidation of
NADH to oxidized nicotinamide adenine dinucleotide (NAD).
Stability: Separated serum may be stored for up to 3-5 days at room temperature or for up to 7 days
at 4°C or stored frozen indefinitely at -20°C.
Comment: BUN/Creatinine Ratio is a calculation and not orderable by itself. Included in the METB,
CMPL, GHP, RPNL, NUTP, OPNL, DPNL, ATPN, TPNL.
Method Calculation
Description:
MERCY TEST NAME BUN POST DIALYSIS MERCY LAB CODE BUNP
Blood samples from some patients with monoclonal gammopathies may produce falsely
elevated results.
Method Urea is hydrolyzed in the presence of water and urease to produce ammonia and carbon
Description: dioxide. The ammonia reacts with 2-oxoglutarate in the presence of glutamate
dehydrogenase and reduced nicotinamide adenine dinucleotide (NADH). The oxidation of
NADH to oxidized nicotinamide adenine dinucleotide (NAD).
POWERCHART BUPRENORPHINE
NAME
MERCY TEST NAME MISCELLANOUS GENERAL LAB Designate: Mayo order MERCY LAB CODE CMIS
code - BUPM
POWERCHART C3 COMPLEMENT
NAME
Stability: Specimens may be stored for up to 3 days at 2–8°C or stored frozen for up to 3 weeks at -
20°C.
Method The Atellica CH Complement C3 (C3) assay measures the concentration of complement C3 in
Description: serum using an immunoturbidimetric assay. The complements are part of a complex
biological system, which works in conjunction with antibody and other factors to protect the
body from invasion of pathogens. When activated by either the classical or alternate
pathway, complements act on biological membranes and may cause cell death. The human
complements consist of several distinct plasma proteins, such as complement C3 and
complement C4.
Stability: Specimens may be stored for up to 3 days at 2–8°C or stored frozen for up to 3 weeks at -
20°C.
Method The Atellica CH Complement C4 (C4) assay measures the concentration of complement C4 in
Description: serum using an immunoturbidimetric assay. The complements are part of a complex
biological system, which works in conjunction with antibody and other factors to protect the
body from invasion of pathogens. When activated by either the classical or alternate
pathway, complements act on biological membranes and may cause cell death. The human
complements consist of several distinct plasma proteins, such as complement C3 and
complement C4.
Stability: Tightly cap and refrigerate specimens at 2–8°C if the assay is not completed within 8
hours. Freeze samples at ≤ -20°C if the sample is not assayed within 24 hours. Thoroughly
mix thawed samples before using.
Comment: Do not interpret levels of CA 125 as absolute evidence of the presence or the absence of
malignant disease. Before treatment, patients with confirmed ovarian carcinoma frequently
have levels of CA 125 within the range observed in healthy individuals. Elevated levels of CA
125 can be observed in patients with nonmalignant disease. Measurements of CA 125
should always be used in conjunction with other diagnostic procedures, including
information from the patient’s clinical evaluation.
The concentration of CA 125 in a given specimen determined with assays from different
manufacturers can vary due to differences in assay methods, calibration, and reagent
specificity. CA 125 determined with different manufacturers’ assays will vary depending on
the method of standardization and antibody specificity. Therefore, it is important to use
assay specific values to evaluate quality control results.
Do not use samples that contain fluorescein. Evidence suggests that patients undergoing
retinal fluorescein angiography can retain amounts of fluorescein in the body for up to 72
hours post-treatment. In the cases of patients with renal insufficiency, including many
diabetics, retention could be longer. Such samples can produce either falsely elevated or
falsely depressed values when tested with this assay and should not be tested.
Patient samples may contain heterophilic antibodies that could react in immunoassays to
give falsely elevated or depressed results. This assay is designed to minimize interference
from heterophilic antibodies.
Method The Atellica IM CA 125II assay is a 2-site sandwich immunoassay using direct
Description: chemiluminometric technology, which uses 2 mouse monoclonal antibodies specific for CA
125. The first antibody is directed toward the M11 antigenic domain and is labeled with
acridinium ester. The second antibody is directed toward the OC 125 antigenic domain and
is labeled with fluorescein. The immunocomplex formed with CA 125 is captured with
mouse monoclonal anti-fluorescein antibody coupled to paramagnetic particles in the Solid
Phase.
Reference
0-31.3 units/mL
Range:
Method The Atellica IM CA 15‑3 assay is a fully automated, 2-step sandwich immunoassay using
Description: direct chemiluminescent technology.
CPT Code: 86300
Method The Atellica IM CA 19-9 assay is a 2-step sandwich immunoassay using direct
Description: chemiluminescent technology that uses a single monoclonal antibody, 1116-NS-19-9, for
both the Solid Phase and Lite Reagent.
CPT Code: 86301
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Cause for
Specimens that have not been aliquoted will be canceled.
Rejection:
POWERCHART CALCITONIN
NAME
MERCY TEST NAME CALCITONIN* MERCY LAB CODE CLCN
Caution: This test is not useful for evaluating calcium metabolic diseases.
Note: Patient preparation: 12 HOURS before this blood test do not take vitamins or dietary
supplements containing
biotin or vitamin B7, which are commonly found in hair, skin, and nail supplements and
vitamins.
Specimen: 1.0 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Place specimen on ice immediately after collection
Performed: 3 days. Test set up Monday through Saturday.
Processing: Refrigerate specimen during centrifugation and immediately transfer serum to plastic
vial. Send frozen to Mayo. Mayo order code (CATN).
Reference: Included in report.
Method: Electrochemiluminescence Immunoassay
CPT code: 82308
Stability: Separated serum/plasma specimens may be stored for up to 8 hours when stored at room
temperature or for at least 2 days at 4°C or stored frozen for at least 6 months at -20°C.
Method Calcium ions form a colored complex with Arsenazo III. The amount of calcium present in
Description: the sample is directly proportional to the intensity of the colored complex formed.
Stability: Urine specimens should be collected in a bottle containing 10 mL of 6 M HCl per 24-hour
specimen to prevent calcium salt precipitation.
Method Calcium ions form a colored complex with Arsenazo III. The amount of calcium present in the
Description: sample is directly proportional to the intensity of the colored complex formed.
Stability: Specimens may be stored for up to 8 hours at 25°C or for up to 2 days at 2-8°C or stored
frozen for up to 30 days at -20°C.
Specimen: Arterial Whole Blood collected in a Heparinized syringe. Minimum volume of 1 ml.
o The tube must be walked to its destination. Do NOT send through the tube
station.
Comment: Respiratory or RN will call lab 87256 to come pick up Arterial specimen that Respiratory is
collecting.
Rejection Criteria: Air in the sample, clotted, hemolyzed, unlabeled specimens
Performed: Within 10 minutes of specimen collection.
Specimen:
Venous Whole Blood collected in a Lithium Heparin tube. Minimum volume 1 ml. Lab may collect.
o The tube must be walked to its destination. Do NOT send through the tube station.
o Testing can be done no matter how old the specimen is. There are no time restrictions for
this test.
o This tube may not be used for other testing.
o Do not open the tube until analysis.
o Serum specimens, SST, and purple top EDTA tubes are unacceptable.
o
Reference Lab Send specimen on ice.
Clients:
Rejection Criteria: Clotted, hemolyzed, unlabeled specimens.
Performed: Within 10 minutes of specimen collection.
Stability: Specimens may be stored for up to 3 days at 20-25°C or for up to 7 days at 2-8°C or stored
frozen for up to 30 days at -20°C or colder
Comment: Panel includes CK, AST, and LD. Hemolyzed specimens are not acceptable.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: Refer to individual tests
Method Refer to individual tests
Description:
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: Send refrigerated to Mayo. Frozen acceptable. Mayo order code (CLPMG).
Performed: 1-2 days. Monday - Saturday; 4 p.m.
Reference Value: Included in report.
Method: Enzyme-Linked Immunosorbent Assay (ELISA)
86147 x 2
CPT Code:
Specimen: 0.5 mL of serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated (preferred) to Mayo. Frozen is also acceptable. Mayo order code (ACLIP).
Performed: Monday, Wednesday, Friday; Report available 3-5 days from receipt.
Reference Value: Included in report.
Method: Enzyme-Linked Immunosorbent Assay (ELISA)
86147
CPT Code:
POWERCHART
CATECHOLAMINE FRACTIONATION URINE
NAME
MERCY TEST NAME CATECH FR 24UR* (alternate name Catecholamine MERCY TEST
CTCH
Fractionation, Free) CODE
Comment:
o A single 24-hour urine collection may be used for CATECHOLAMINE
FRACTIONATION, METANEPHRINES [METN24U] and VMA [VMA24UR].
o The specimen must be kept refrigerated during collection.
Patient o This assay is of most value when the specimen is collected during a hypertensive
preparation: episode.
o Discontinue any epinephrine, norepinephrine, or dopamine injections/infusions at
least 12 hours before specimen collections, UNLESS drug monitoring is the goal.
o Discontinue drugs that release or hinder metabolism of epinephrine,
norepinephrine or dopamine for at least 1 week before obtaining the specimen. If
this is not possible for medical reasons, contact Mayo laboratory to discuss
whether a shorter drug-withdrawal period may be acceptable.
o Do not perform the test on patients withdrawing from legal or illegal drugs known
to cause rebound plasma catecholamine release during withdrawal.
Cautions: o Many alterations in physiologic and pathologic states can profoundly affect
catecholamine concentrations.
o Any environmental factors that may increase endogenous catecholamine
production should be avoided. These include noise, stress, discomfort, body
position, and the consumption of food, caffeinated beverages, and nicotine.
Caffeine and nicotine effects are short term, a few minutes to hours only.
o Other substances and drugs that may affect the results include:
Substances that result in increased release or diminished metabolism of
endogenous catecholamines
Monoamine oxidase inhibitors (MOLs)-a class of anti-depressants with marked
effects on catecholamine levels, particularly if the patient consumes tyrosine rich
foods, such as nuts, bananas , or cheese.
Catecholamine reuptake inhibitors including cocaine and synthetic cocaine
derivatives, such as many local anesthetics, which also can be antiarrhythmic drugs
(e.g., lidocaine)
Some anesthetic gases, particularly halothane.
Withdrawal from sedative drugs, medical or recreational.
Vasodilatin drugs (e.g., calcium antagonists, alpha-blockers)
Tricyclic antidepressants usually exert a negligible effect.
o Substances that reduce or increase plasma volume acutely (e.g., diuretics,
radiographic contrast media, synthetic antidiuretic hormone {e.g., desmopressin 1-
deamino-8-d-arginine vasopressin: DDAVP})
Specimen: o Before start of collection, add 25 ml 50% acetic acid preservative to the container
(15 ml 50% acetic acid for children
o Collect for 24 hours.
o RL Clients, please call Mercy Lab to have a jug prepared with preservative.
o Refrigerate during collection.
Reference Lab: Adjust pH to 2.0-4.0 with 50% acetic acid. Aliquot 20 ml and indicate the 24-hour volume.
Processing: o Separate aliquots must be submitted for Metanephrines and VMA if collected with
this specimen.
o Identify which specimen is for Catecholamine Fractionation.
Mercy lab o 2 ml in a 10 ml urine tube. Mayo order code (CATU).
Processing: o Send refrigerated to Mayo. Frozen acceptable. Ambient with preservative
acceptable.
Performed: 2-4 days. Test set up Monday through Saturday.
Reference Value: Included with test results.
Method: High Performance Liquid Chromatography (HPLC)
CPT Code: 82384
MERCY TEST NAME CATHETER TIP CLT MERCY LAB CODE CTC
Order: Specify site of insertion (subclavian, peripherally inserted central catheter, etc.)
Comments: oQuantitation will be reported for each isolate. >15 colony forming units (CFU) is
considered significant.
o Susceptibility testing will be performed on significant isolates.
Method: Standard culture techniques
POWERCHART CBC
NAME
No differential included.
Comment: Cell morphology will be ordered and charged if established criteria/diagnosis are met.
Specimen: 1 purple top (EDTA) tube.
Stability: 24 hours room temp, or 48 hours refrigerated.
Performed: Within 8 hours of receipt. Available stat.
Reference value: Included with test results. Complete listing in Special Helps section of Lab Test Index.
Method: Automated cell counter.
CPT Code: 85027
MERCY TEST NAME CBC with Diff MERCY LAB CODE CBCAD
MERCY TEST NAME CBC Diff MANUAL MERCY LAB CODE CBCD
Cell morphology will be ordered and charged if established criteria/diagnosis are met.
POWERCHART
CD4
NAME
MERCY TEST NAME CD4 T-CELL COUNT* MERCY LAB CODE CD4A
POWERCHART CEA
NAME
MERCY TEST NAME CEA* MERCY LAB CODE CEA
Method The Atellica IM CEA assay is a 2-site sandwich immunoassay using direct chemiluminometric
Description: technology which uses constant amounts of 2 antibodies.
Patient Fasting is recommended to avoid lipemia which may interfere with the test.
Preparation:
Specimen: 0.5 ml serum for IgA and 1.0 ml serum for TISTA from a Serum Separator Tube (SST) or no
additive serum tube. Send refrigerated
Cause for
Hemolysis or gross lipemia
Rejection:
Comment: TISTA is a new assay that performs the Tissue Transglutaminase and deamidated Gliadin
simultaneously for IgA. There is no need for a separate order for the tTG and deamidated
gliadin. Ordering the TISTA will cover for both assays, simultaneously, but will be reported as
one result for IgA and one result for IgG
Processing: IgA can be refrigerated for up to 21 days. Send TISTA refrigerated to Mayo. (Mayo order
code (TSTGP) Frozen is acceptable for both.
Performed: IgA-Within 8 hours of receipt
TISTA-Monday thru Saturday at Mayo
Reference Value: Included with the report
Method: IgA-Immunoturbidimetric
TISTA-EIA
CPT Code: 82784-IgA
83516x2-TISTA
MERCY TEST NAME CELL COUNT CSF MERCY LAB CODE CCSF
POWERCHART
CELL MORPHOLOGY
NAME
POWERCHART CERULOPLASMIN
NAME
Specimen: 1 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
MERCY TEST NAME CHLAMYDIA PNEUMONIAE BY PCR* MERCY LAB CODE MISM
MERCY TEST MISCELLANEOUS GENERAL LAB Designate: Mayo order MERCY LAB CODE CMIS
NAME code - MCRNA
Specimen: Swab specimen collected using the APTIMA Collection Vaginal Swab (the APTIMA Unisex
Swab can also be used). Collection kits are available from Mercy Lab.
Mayo approved: The following sites are approved for Chlamydia testing at Mayo Med Labs, ONLY (Mercy Lab
is not approved to do testing on these sites):
Sites: o Rectal/anal
o Ocular (corneal/conjunctiva)
o Oral/throat
o Pelvic wash, cul-de-sac fluid (this source requires the APTIMA specimen transfer
tube T652, available from Mercy lab).
NOTE: If provider wants both Chlamydia and GC testing done on rectal, ocular, oral or
pelvic, a separate order will have to
be placed for each test.
Cause for o Transport tubes that are received without collection swabs inside.
rejection: o Transport tubes that have expired.
o Transport tubes received with a swab different from the one provided in the
collection kit.
o Sources other than those listed above.
Comment: In the case of suspected child abuse, culture is the only recommended procedure.
See: Chlamydia Trachomatis Culture listed below
RL Client o If ordering the test at your facility, order a CMIS and put in comment the test is for
Comments: MCRNA and include the source (rectal, ocular, oral, pelvic). If you will order using a
requisition, write CMIS on the order form and indicate the testing is for MCRNA
and include the source (rectal, ocular, oral, pelvic).
o Send the APTIMA transporter refrigerated to Mercy lab.
Processing: Refrigerate sample after collection and sent to Mayo Med Labs refrigerated. Mayo order
MCRNA (C. trach, Misc., Amplified RNA)
Thawed or frozen specimens which are turbid must be clarified by centrifugation prior to
Comment: testing
Performed: Within 8 hours of receipt. Available stat.
Reference
97 - 109 mmol/L
range:
Method The methods for measurement of electrolytes include flame photometry, spectrophotometry
Description: and direct or indirect ion selective electrode potentiometry. The A-LYTE Na, K, and Cl assays
are indirect Integrated Multisensor Technology (IMT). There are four electrodes used to
measure electrolytes. Three of these electrodes are ion-selective for sodium, potassium, and
chloride. A reference electrode is also incorporated in the multisensor.
CPT Code: 82435
Specimen: 5 mL unpreserved urine from a 24-hours urine collection that was refrigerated during
collection.
Stability: Twenty-four-hour collection should be made without addition of preservatives and stored
refrigerated at 2-8°C or frozen for delayed analysis.
Comment: Indicate volume of urine collection
Performed:
Within 8 hours of receipt. Available Stat.
Reference Range: 110-250 mmol/L/24 Hours
Method: The A‑LYTE Na, K, and Cl assays use indirect Integrated Multisensor Technology (IMT). There
are four electrodes used to measure electrolytes. Three of these electrodes are ion-selective
for sodium, potassium, and chloride. A reference electrode is also incorporated in the
multisensor.
CPT Code: 82436
POWERCHART CHROMOGRANIN A
NAME
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Comment: Proton pump inhibitor drugs should be discontinued at least 2 weeks before collection.
MERCY TEST NAME CHRM ANLYS BLD* MERCY LAB CODE CHRB
Specimen: 5-10 ml whole blood collected in yellow top (ACD) (Preferred), Sodium heparin,
o
EDTA tubes
Processing: o Send WHOLE BLOOD. DO NOT CENTRIFUGE.
o Send ambient to Mayo. DO NOT FREEZE. Refrigerated also acceptable. Mayo Code
(CHRHB) for Chromosome Analysis, Hematologic Disorders, Blood.
o Mercy Lab staff will order on Mayo Access
o Reason for referral will need to be entered on Mayo Access when ordering.
Performed: Monday – Friday 9-11 days
Reference value: An interpretive report will be provided.
CPT Code: 88237, 88291- Tissue culture for neoplastic disorders; bone marrow, blood, Interpretation
and report
88264 w/ modifier 52-Chromosome analysis with less than 20 cells (if appropriate)
88264-Chromosome analysis with 20 to 25 cells (if appropriate)
88264,88285- Chromosome analysis with greater than 25 cells (if appropriate)
88283-Additional specialized banding technique (if appropriate)
MERCY TEST NAME CHRM CONGENITAL BLOOD* MERCY LAB CODE CHRC
CPT Code: 88230 - Tissue culture for chromosome analysis (if appropriate)
88262 - With modifier 52 (if appropriate)
88291
88280
88283 - (if appropriate)
MERCY TEST NAME CHRM ANLYS BM* MERCY LAB CODE BMC
Specimen: o 2-3 ml of bone marrow placed in yellow top (ACD), sodium heparin, or EDTA tubes
Comment: o Complete the Hematopathology portion of Mayo Connect Additional Test
Information form.
o Send a copy of CBC and/or bone marrow report.
o Chromosome analysis is not recommended for plasma cell neoplasms due to
limited clinical utility; therefore effective 12/7/2020, Mayo will cancel the BMC
(Mayo CHRBM) if ordered concurrently with a plasma cell FISH tests such as Mayo's
PCPDS, MSMRT or MFCF
Processing: o See Mayo Test Catalog for complete instructions.
o Send ambient to Mayo. Refrigerated acceptable. Mayo CHRBM
Cause for
Specimen sent frozen will be rejected.
Rejection:
Performed: 9-11 days. Samples processed Monday through Sunday
Reference value: Interpretation included with test results.
Method: Cell culture without mitogens followed by chromosome analysis.
CPT Code: 88237, 88291-Tissue culture for neoplastic disorders; bone marrow, blood, interpretation,
and report
88264 w/ modifier 52-Chromosome analysis with less than 20 cells (if appropriate)
88264-Chromosome analysis with 20 to 25 cells (if appropriate)
88264, 88285-Chromosome analysis with greater than 25 cells (if appropriate)
88283-Additional specialized banding technique (if appropriate)
MERCY TEST NAME CITRATE EXCRT 24UR* MERCY LAB CODE CITRAT
Patient Any drug that causes alkalemia or acidemia may be expected to alter citrate excretion and
preparation: should be avoided, if possible. The patient must avoid laxative use for 24-hour collection
period.
Tightly cap and refrigerate specimens at 2-8°C if the assay is not completed within 4 hours.
Stability: Freeze samples at ≤20°C if the sample is not assayed within 48 hours. Freeze samples only
1 time and mix thoroughly after thawing.
Comment: Do not use samples that have been stored at room temperature for longer than 4 hours.
Performed: Within 8 hours of receipt. Available Stat.
MERCY TEST NAME CLOSTRIDIUM DIFFICILE TOXIN GENE MERCY LAB CDIFFM
CODE
Test stool for C. difficile on all patients with clinically significant diarrhea AND history of
recent antibiotic use or exposure to C. difficile.
Specimen: FRESH SPECIMEN ONLY:
POWERCHART
NAME
MERCY TEST NAME CLOSTRIDIUM DIFFICLE TOXIN AND ANTIGEN MERCY LAB CTOXR
CODE
Comment: To be used only be our regional hospital Community Works sites or for regional hospital
correlation purposes. These order / results are not available within Cerner Powerchart. This
is NOT PCR or molecular testing.
For all other testing purposes, see Clostridium difficile molecular / CDIFFM.
Performed: Daily
MERCY TEST NAME ACTIVATED CLOTTING TIME MERCY LAB CODE ACTLT
Specimen: 2 mL Serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: Send refrigerated to Mayo. Mayo code -(COXIS)
Comment: If this initial Coccidioides Antibody testing is positive then additional reflex testing (Mayo
code - RSCOC) Coccidioides Antibody by Complement Fixation, and Immunodiffusion (IgG,
IgM) will be performed at an additional charge.
MERCY TEST NAME COLLECT CHG CBG MERCY LAB CODE CCBG
Specimen: o The patient’s heel or finger must be warmed prior to specimen collection.
o Refer to Phlebotomy Procedure Manual for complete specimen collection
instructions.
Comment: o Available stat.
o Included in the capillary venous blood gas order set.
o Outpatients-order CBGCVP for the blood gas test along with the CCBG for collect
charge.
o Lab collects and testing performed by CV&P.
o This can NOT be used for venous collections.
Method: Heel stick, Fingerstick
CPT Code: 36416
MERCY TEST NAME COLLECT CHG DONOR MERCY LAB CODE MDONOR
Specimen: 1 ml serum from a no additive serum tube or Serum Separator Tube (SST).
Stability: Immediately after drawing the specimen, place the tube on wet ice.
Processing: o Separate from clot and freeze immediately.
o Send frozen to Mayo. Mayo order code (COM).
Performed: 2 days. Test set up Monday through Friday; 3 p.m..
Reference value: Included in report.
Method: Automated Liposome Lysis Assay
CPT Code: 86162
MERCY TEST NAME COMP METABOLIC PNL MERCY LAB CODE CMPL
Specimen: 1 ml of serum
Stability: Specimens may be stored for up to 3 days at 2-8°C or stored frozen for up to 60 days at -
20°C.
Avoid hemolyzed samples for potassium. Hemolyzed samples may give incorrect elevated
Comment:
potassium. Panel includes Glucose, BUN, Creatinine, BUN/Creatinine Ratio, eGFR, Total
Bilirubin, Total Protein, Albumin, A/G Ratio, Calcium, Alkaline Phosphatase, AST, ALT, Sodium,
Potassium, Chloride, CO2, Anion Gap
Performed: Within 8 hours of receipt. Available Stat.
Reference Range: See individual test entry.
Method
See individual test entry.
Description:
CPT Code: 80053
MERCY TEST NAME CONN TIS DIS CASC* MERCY LAB CODE CTDC
MERCY TEST NAME COOMBS DIRECT (DAT (DIRECT ANTIGLOBULIN TEST) MERCY LAB CODE CMBS
Comment: For newborns: Order a Cord Blood Routine whenever a Direct Coombs is needed if the cord
blood is available, and this is the initial Direct Coombs order.
Specimen: One 6 ml pink top tube or purple top tube. Do not spin. Refrigerate.
Performed: Within 8 hours of receipt. Available stat.
Reference value: Negative
Method: Serological
CPT Code: 86880
Specimen: o Draw before any other tubes are drawn. 0.8 ml serum from Navy blue monoject-
no additive, trace element blood collection tube.
o Use alcohol, not iodine to cleanse venipuncture site.
Cause for
The use of other tubes is unacceptable.
rejection:
Processing: o Allow to clot well (for at least 30 minutes before spinning). Then centrifuge the
specimen to separate serum from the cellular fraction. Serum must be removed
from the cells within 4 hours of specimen collection. Pour serum into a
Mayo Metal FREE vial. Do NOT use a transfer pipet or wooden sticks. Avoid
hemolysis.
o Send to Mayo refrigerated. Ambient acceptable. Mayo order code (CUS1).
Performed: 1-3 days. Monday through Saturday.
Reference value: Included with report
Method: Dynamic Reaction Cell Inductively Coupled Plasma Mass Spectrometry (DRC-ICP-MS)
CPT Code: 82525
Specimen: o 5-10 ml whole blood collected from the umbilical cord. Blood is to be placed in
a red top tube and purple top tube. Refrigerate.
o NOTE: Tubes must be labeled with baby's identification, mother's FULL name, date
and time of delivery.
Comment: o Enter mother's FULL name in comment field.
o Includes ABO Group/RH Type and Direct Coombs (DAT).
o If the Direct Coombs is positive, Lab will order and charge for a CBC, Cell
Morphology, Bilirubin from the cord blood and Antibody ID from the eluate.
Performed: Within 8 hours of receipt. Available stat.
POWERCHART
CORONAVIRUS (COVID-19/SARS-CoV-2) POCT
NAME
MERCY TEST NAME COVID 19 SARS COV2 MERCY LAB CODE COV19P
Specimen: Two Nasal Swab in Sterile conical tube, each collected from both nares.
Request ID NOW Covid collection kit from Lab.
CPT Code:
87635
POWERCHART
CORONAVIRUS, FLU A/B, RSV Panel
NAME
MERCY TEST NAME COVID, FLU A/B, RSV MERCY LAB CODE
CEPH41
Specimen: Nasopharyngeal swab specimen is collected and placed into a viral transport tube containing
3 mL transport medium. The specimen is briefly mixed by rapidly inverting the collection
tube 5 times (Preferred).
Nasal swab placed in 3 mL of viral transport media.
Mid-Turbinate Swab placed in 3 mL of viral transport media.
Nasal Wash/Aspirate.
Processing: Nasal Wash/Aspirate-using a clean 300 uL transfer pipette (supplied), transfer 600 uL of the
sample (two draws using the same transfer pipette) into the 3 mL transport medium tube
then cap the tube.
CPT Code:
U0241
POWERCHART
NAME Coronavirus and Influenza A/B Panel (COVID AND FLU A/B AG) **For Clinic Use Only***
MERCY TEST
MERCY LAB CODE
NAME COVID AND FLU A/B AG SARFLU
POWERCHART
NAME SARS CoV2 COVID Coronavirus Antibody IgG
MERCY TEST
MERCY LAB CODE
NAME SAR COVID 2 IGG AB COR2G
MERCY TEST
MERCY LAB CODE
NAME COVID19 AG POCT COVAGP
MERCY TEST NAME CORTISOL ACTH RES MERCY LAB CODE CORT 3 orders
3 separate specimens, requiring 3 separate CORT orders, one prior to and two following
injection of 0.25 mg Cortrosyn, given IV bolus, at times specified by Nursing Service.
Stability: Tightly cap and refrigerate specimens at 2–8°C if the assay is not completed within 8 hours.
Freeze samples at ≤ -20°C if the assay is not completed within 48 hours.
Freeze samples only 1 time and mix thoroughly after thawing.
Comment: Mercy lab clients: Testing is done in the Mercy Cancer Center. Ordering clinic will fill out
form OMH-146, following the directions on the form for the information required. Fax order
and accompanying information to Mercy First Call at 641-428-6140, who will fax the
information to Mercy's Cancer Center. Mercy Cancer Center will schedule the appointment
and call the patient with instructions.
Reference Range: Expected values during ACTH stimulation: over twice (usually 2-3 times) reference a.m. level.
Method The Atellica IM Cor assay is a competitive immunoassay using direct chemiluminescent
Description: technology
CPT Code: 82533x3
POWERCHART C-PEPTIDE
NAME
Specimen: 1 mL of serum
Stability: Do not use samples that have been stored at room temperature for longer than 8 hours.
Separate serum from the red blood cells before storage at 2–8°C or -20°C. Tightly cap and
refrigerate specimens at 2–8°C if the assay is not completed within 8 hours. Freeze samples
at ≤ -20°C if the assay is not completed within 24 hours. Freeze samples only 1 time and mix
thoroughly after thawing.
Reference Range: Female: 0.730-4.370 ng/mL
Male: 0.81-3.85 ng/mL
Method The Atellica IM CpS assay is a 2-site sandwich immunoassay using direct chemiluminescent
Description: technology which uses constant amounts of 2 antibodies
CPT Code: 84681
POWERCHART CREATININE
NAME
MERCY TEST NAME CREATININE (CREAT AND GFR) MERCY LAB CODE CREAT
Method Description: The Atellica CH Enzymatic Creatinine_2 (ECre_2) assay is based on the enzymatic reaction.
CPT Code: 82565
Stability: Specimens may be stored for up to 2 days at 2–8°C or stored frozen at or below -20°C.
Performed: Within 8 hours of receipt. Available stat
Reference Range: No reference range established
Method
The Atellica CH Enzymatic Creatinine_2 (ECre_2) assay is based on the enzymatic reaction.
Description:
CPT Code: 82570
Specimen: 5 mL of unpreserved urine from a 24-hour urine specimen that was refrigerated during
collection.
Stability: Urine specimens may be stored for up to 4 days at 2-8°C or stored frozen at or below -20°C.
Comment: Includes volume (ml/24 hours) and calculated creatinine (g/24 hours)
Performed: Within 8 hours of receipt. Available Stat
Reference Range: Creatinine Male Female
0.8-2.8 0.8-2.8 g/24 hrs.
g/24 hrs.
Method The Atellica CH Enzymatic Creatinine_2 (ECre_2) assay is based on the enzymatic reaction.
Description:
CPT Code: 82570
5 mL of unpreserved urine from a 24-hour urine specimen that was refrigerated during
collection.
Stability: Urine specimens may be stored for up to 4 days at 2–8°C or stored frozen at or below -20°C
Comment: o Outpatients and Inpatient, Mercy Laboratory will order the appropriate serum
creatinine (CRTMM) if a serum creatinine has not been completed within 48 hours.
This will be done at no additional charge. The patient needs to have blood drawn
when the container is picked up or delivered. In order to avoid possible duplication,
the serum creatinine is not to be ordered by the physician office, the hospital floor or
admitting.
o Regional Lab Clients, send 0.5 ml serum for the creatinine at the same time that the
urine specimen is sent. This enables analysis of both specimens by the same method
for accuracy.
Mercy Laboratory will order the serum creatinine at no charge. Do not order a single
creatinine on the requisition.
Includes Volume (ml/24 hours) Raw Creatinine (mg/dl) and Calc. Creatinine (g/24 hours)
Creatinine Clearance (ml/min)
MERCY TEST NAME CROSSMATCH (Type and Cross) MERCY LAB CODE XMI
A check mark MUST be put by the Medical Record number on the tubes drawn for a
Crossmatch by the person drawing the specimen indicating the phlebotomist has
matched the medical record number on the Specimen with the medical record
number on the Patient Armband and it is identical along with the name and other
pertinent information. Date, time, and initials of the individual collecting the specimen
must be on the tube.
Processing: Regional Lab Clients: Crossmatch verification by transfusing facility is recommended for all
units crossmatched at Mercy.
Performed: Within 8 hours of receipt. Available stat.
Method: Serological
CPT Code: 86900 ABO+
86901 RH+
86850 Antibody Sc
86920 Unit Compatibility (1 for each unit ordered)
MERCY TEST NAME CRP SENS (CARDIAC) MERCY LAB CODE HSCRP
MERCY TEST NAME CRYOGLOBULIN* (CRYOGLOBULIN AND MERCY LAB CODE CRYG
CRYOFIBRINOGEN PANEL)
Specimen: 5 ml serum from a no additive serum tube plus 1 ml EDTA plasma. Minimum 3 ml serum
and 0.5 ml plasma. Testing requires both specimens.
Processing: o Deliver to Lab immediately!
o Keep specimens at 370C, 98.60F until delivered, by holding tubes in hands, may
wrap tubes in a heel warmer. Place plasma and serum in appropriately labeled
plastic vials and mark each corresponding aliquot as serum or plasma.
o Regional Lab Clients: Keep specimens at 370C, 98.60F until the plasma and serum
are removed from the cells. It is very important that the specimen remain at 37
degrees C until after separation of plasma/serum from red cells. Place plasma and
serum in appropriately labeled plastic vials and mark each corresponding
aliquot as serum or plasma.
o Send refrigerated. Frozen acceptable. Mayo order code (CRGSP).
Cause for
A SST tube is not acceptable.
rejection:
Performed: 2-10 days. Test set up Monday through Friday.
Reference value: Included with report.
Method: Quantitative and Qualitative typing. Precipitation at 1°C. Includes cryofibrinogen.
CPT Code: 82595 Cryoglobulin +*
82585 Cryofibrinogen +*
MERCY TEST NAME CRYO FOR INFUS MERCY LAB CODE CRYO
MERCY TEST NAME CRYO NOT FOR INFUS MERCY LAB CODE CRYX
Comment: o To be ordered by Nursing Service at the same time an order is placed to Pharmacy
for Fibrin Glue.
o One order of Cryoprecipitate is necessary for each unit of Fibrin Glue requested.
o Used in the preparation of Fibrin Glue, a topical hemostatic agent used in surgery.
Specimen: None needed
Processing: Group specific cryoprecipitate is not needed.
Performed: Allow 10-30 minutes thawing time. Available stat.
Method: Thawed.
CPT Code: P9012 Cryoprecipitate (Proc)* (1 for each unit ordered)
Specimen: 1 mL serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: Send Refrigerated to Mayo, Mayo code - (SLFA)
If this initial Cryptococcus Antigen testing is positive, then additional reflex testing (Mayo
Comment: code - SLFAT) Cryptococcus Antigen Titer by Later Flow Assay (LFA) will be performed at an
additional charge.
1-2 days. Monday through Friday: 11 a.m.
Performed:
Saturday, Sunday; 1 p.m.
Included in report
Reference value:
Method: Lateral Flow Assay
87899 Cryptococcus Ag Screen
CPT Code:
87899 Cryptococcus Ag Titer (if applicable)
Comment: o CSF Differential is included in Cell Count CSF if ≥ 6 WBC/mcl are present.
o To be ordered by Regional Hospitals when they are doing the cell counts at their
facility and want to refer the differential to Mercy.
Specimen: o Send 2 cytocentrifuge prepared slides, unstained.
o If a cytocentrifuge is not available, mix 1 drop of 22% albumin with 3-5 drops of
CSF. Place a drop on the slide and allow to air dry, do not spread.
Stability: 1 hour room temp
Cause for o Up to 40% of cells in CSF lyse within 1 hour after collection.
rejection: o It is not acceptable to send CSF fluid.
Performed: Within 8 hours of receipt. Available stat.
Reference values: Age Neutrophil Lymphocyte Monocyte
0 - 1 year 0 - 8% 5 - 35% 50 - 90%
> 1 year 0 - 6% 40 - 80% 15 - 45%
Method: Microscopic exam of Wright-stained smear.
CPT Code: NA
POWERCHART CS (Cardiac Surgery) PANEL
NAME
Comment: Orderable only by Cardiac Surgery Personnel. Used for specimens collected outside of the
open-heart surgery suite (OR10).
Includes: Hemogram Glucose Ionized calcium
Potassium Sodium
Specimen: 2 ml whole blood from purple top tube AND 0.5 ml whole blood from green top (lithium
heparin) tube without gel.
Reference value: See individual test entry
Method: o Sodium, Potassium, Ionized calcium by direct ion selective electrode
potentiometry.
o Glucose by amperometrically.
o Hemogram by automated cell counter.
CPT Code: 85027 Hemogram
82947 Glucose
84132 Potassium
84295 Sodium
82330 Calcium, Ionized
MERCY TEST NAME CUTAN IMMU BIOP* MERCY LAB CODE CUTBX
Specimen: 4 MM punch biopsy of recent lesion and small portion of normal tissue placed into Mayo's
special transport media.
Comment: If a specimen is to be sent to pathology in addition to a specimen sent for Mayo testing, fill
out both the pink Pathology /Dermatology Request Form and the Mayo Additional test
Information form. Include patient's age, sex, diagnosis, biopsy site, sun exposure of
specimen (exposed, unexposed) and involvement of specimen (perilesional, involved,
uninvolved).
Processing: Send Ambient (Frozen and refrigerated specimens are acceptable) to Mayo with request
form. Mayo order code (CIB).
Performed: 1-3 days. Test set up Monday through Friday
Reference value: Included in report
Method: Direct Immunofluorescence staining of cryostat prepared skin biopsy sections for IgG, IgM,
IgA, C3, and Fibrinogen deposition.
CPT Code: 88346
88350 x5
MERCY TEST NAME CUTAN IMMU IGG* MERCY LAB CODE CUT
Specimen: 2 ml serum from a SST or plain red top tube. Minimum 0.5 ml.
Processing; Send refrigerated to Mayo in a screw cap plastic vial. Ambient or frozen acceptable. Mayo
order code (CIFS).
Performed: 2-7 days. Test set up Monday through Friday; 7 a.m.-5 p.m.
Reference value: Included in report.
Method: Detection of IgG anti-intercellular substance (ICS) and anti-basement membrane zone (BMZ)
antibodies by indirect immunofluorescence technique using Rhesus monkey esophagus
substrate and human NaCl split-skin substrate. Serum is tested for presence and titer of
antibodies. Titer is obtained on monkey esophagus substrate, and pattern of BMZ
fluorescence is determined on split-skin substrate.
MERCY TEST NAME CYCLOSPORIN STAIN (stool specimen required) MERCY LAB CODE CYSTN
o Transfer enough stool specimen to bring the liquid level up to the fill line indicated
on the ECOFIX
preservative. DO NOT OVERFILL.
o Mix thoroughly. Pieces should be pea size or less.
o Send ambient.
Unpreserved stool:
Comment: o Occasionally patients will come in with orders to have their Cyclosporin sent to
another reference Lab.
o Follow the instructions the patient presents for specimen collection and
transportation.
o These patients have a “processing charge” ordered.
o Please include time and date of last dose.
Specimen: 3 ml EDTA (purple top) whole blood. Minimum 1 ml. Do not spin down.
Processing: Send refrigerated to Mayo. Send specimen in original collection tube. Mayo order code
(CYSPR).
Performed: 1 day. Test set up at Mayo Monday through Sunday.
Reference value: Included in report
Method: High Performance Liquid Chromatography/Tandem Mass Spectrometry (Hplc-ms/ms)
CPT Code: 80158
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Performed: 1-3 days. Test set up Monday through Sunday.
Processing: Centrifuge and aliquot serum into a plastic vial within 2 hours of collection. Send
refrigerated to Mayo. Mayo order code (CSTCE).
Method: Immunoturbidimetric
MERCY TEST NAME CYSTIC FIB MUT ANL* MERCY LAB CODE CFMA
Patient A previous bone marrow transplant from an allogenic donor will interfere with testing. Call
Preparation: 800-533-1710 for instructions for testing patients who have received a bone marrow
transplant.
Specimen must arrive to Mayo labs within 96 hours of collection. Specimens can only
be sent Monday through Thursday.
Send to Mayo ambient. Frozen and refrigerated also acceptable. Mayo order code (CFMP).
Additional To ensure minimum volume and concentration of DNA is met, the preferred volume of
Information: blood must be submitted. Testing may be cancelled if DNA requirements are inadequate.
MERCY TEST NAME CYTOKINE PANEL 13* MERCY LAB CODE FCYTP
Specimen: 1 mL serum from a serum gel tube. Plain red top tube is acceptable.
Processing: Spin down within 2 hours and send 1 mL of serum Frozen to Mayo. Mayo order code
(FCYTP).
Performed: 1-8 days, performed Monday, Wednesday, Friday
Reference Value: Included in report
Method: Quantitative Multiplex Bead Assay
CPT Code: 83520 x 12
83529
POWERCHART CYTOMEGALOVIRUS (CMB) ANTIBODY IgG & IgM
NAME
MERCY TEST NAME CMV AB, IGG/IGM QN * MERCY LAB CODE CMVGM
Specimen: Must indicate specimen source. Submit only 1 of the following specimens:
Body fluid (Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular) – 0.5 mL of
fluid in a sterile, screwcap, 5-mL aliquot tube (preferred) or sterile container. Do not
centrifuge. Send to Mayo in Sarstedt Aliquot Tube (T914) or sterile container.
Genital swab (Cervix, vaginal urethra, anal/rectal, or other genital sources) – Culturette (BBL
Culture Swab) (T092). Send swab to Mayo in multimicrobe media (M4-RT, M4 or M5).
Swab (Dermal, eye, nasal, saliva, or throat) – Culturette (BBL Culture Swab) (T092). Send
swab to Mayo in multimicrobe media (M4-RT, M4 or M5).
Tissue (Brain, colon, kidney, liver, lung, etc.) - Whole collection in a Multimicrobe media (M4-
RT) (T605) (Preferred) or Sterile container with 1–2 mL of sterile saline. Submit only fresh
tissue.
Bone Marrow – 0.5 mL of bone marrow in an EDTA tube. Send bone marrow in original
tube. Do not aliquot.
Cautions: A negative result does not eliminate the possibility of cytomegalovirus (CMV) infection.
This assay is only to be used for patients with a clinical history and symptoms consistent
with CMV infection and must be interpreted in the context of the clinic picture.
Processing: Send refrigerated (preferred) to Mayo. Frozen is also acceptable. Mayo order code (CMVPV).
MERCY TEST NAME CYTOPLASMIC NEUT AB* MERCY LAB CODE ANCA
Specimen: 0.5 ml serum from a SST tube or plain red top tube. Minimum 0.4 ml.
Processing: Send refrigerated to Mayo. Frozen is acceptable. Mayo order code ANCA.
POWERCHART D-DIMER
NAME
Specimen: Draw a blue top tube (3.2% citrate) filled appropriately with amount of blood listed on label.
Stability: 4 hours room temp, freeze if > 4 hours, good for 4 weeks frozen.
Cause for
Improperly filled tubes will NOT be tested. Avoid gross hemolysis.
rejection:
Processing: Processing:In-house patients: Centrifuge immediately. Test within 4 hours of collection.
If testing will be delayed longer than 4 hours. Double spin coagulation specimens to ensure that
all platelets are removed and freeze.:
o Centrifuge specimen. Aliquot plasma (leaving some above the cells) to a plastic
centrifuge tube.
o Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the
tube) to another plastic aliquot tube.
o Store plasma in freezer. Label aliquot vial "CITRATED PLASMA."
o Centrifuge immediately.
o Aliquot specimen (leaving some above the cells) to a plastic centrifuge tube.
o Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the
tube) to another plastic aliquot tube.
o Send refrigerated if testing can be performed within 4 hours of collection.
o If testing will not be performed within 4 hours freeze specimen and send frozen.
o Label aliquot vial "CITRATED PLASMA."
Elevated levels of DDIMER are found in clinical conditions such as DVT, PE, and DIC. DDIMER
levels also rise during normal pregnancy, but very high levels are associated with
complications.
MERCY TEST NAME DERM PANEL (Accutane Panel) MERCY LAB CODE ATPN
Stability:
Specimens may be stored for up to 2 days at 2–8°C or stored frozen at or below -20°C.
Comment: Includes Alk Phos, ALT, AST, BUN, BUN/Creat ratios, Cholesterol, Creatinine, eGFR, Glucose,
Total Protein, Triglyceride.
Processing: o Specimen to be collected in dermatology office and inoculated directly to DTM agar.
o The specimen should be sent at room temperature to Mercy lab.
Stability: Tightly cap and refrigerate specimens at 2–8°C for no longer than 6 days if the assay is not
completed within 4 hours. If longer storage is necessary, freeze samples at ≤ -20°C for up to
1 month. Do not store in a frost-free freezer. Freeze samples only 1 time and mix thoroughly
after thawing.
Comment: Test samples as soon as possible after collecting. Do not use samples stored at room
temperature for no longer than 4 hours.
Specimen: 1 ml serum
Stability: Specimens may be stored for up to 2 days at 2–8°C or stored frozen at or below -20°C
Comment: For use by Dialysis Unit only. Includes A/G Ratio, Albumin, Alkaline Phosphatase
AST(SGOT), BUN, BUN/Creatinine Ratio, Calcium, CO2, Creatinine, eGFR, LDH, Phosphorus,
Potassium, Sodium, and Total Protein
Performed: Within 8 hours of receipt. Available Stat.
Reference Range: See individual test entry
Method
See individual test entry
Description:
CPT Code: See individual test entry
Specimen: 2 Blue top tubes (3.2% Citrate) filled appropriately with amount of blood listed on label.
Stability: 4 hours room temp, freeze if >4 hours, good for 4 weeks frozen.
Cause for
Gross hemolysis. Improperly filled tubes will not be tested.
rejection:
Processing: o Centrifuge immediately.
o Separate plasma within 2 hours of collection.
o Double spin and freeze plasma if testing delayed longer than 4 hours.
o Label frozen vial “Citrated Plasma.”
Preformed: Within 8 hours of receipt. Available stat.
Method: Photo-optical clot detection
CPT Code: 85380 D-Dimer
85610 PT
85384 Fibrinogen
85730 PTT
POWERCHART DIFFERENTIAL
NAME
MERCY TEST NAME DIRECT LDL CHOL (Low Density Liopro MERCY LAB CODE DLDL
The National Cholesterol Education Program of the National Heart, Lung, and Blood Institute
has announced the following guidelines:
Optimal: <100mg/dl
Near Optimal: 100-129mg/dl
Borderline high: 130-159mg/dl
High: 160-189mg/dl
Very High: ≥190mg/dl
Method The Atellica CH LDLC assay is a homogeneous assay for directly measuring LDL-C levels in
Description: serum
CPT Code: 83721
MERCY TEST NAME COLLECT CHG DONOR MERCY LAB CODE MDONOR
Comment: o When a potential bone marrow, tissue, or organ donor comes to the lab to be
drawn for compatibility, we will do the collection at no charge to the donor.
o DO NOT add a collect charge or a processing charge.
o The test code "MDONOR" is ordered simply to track that the patient did have a
specimen drawn, but there is no charge associated with the test.
o Patient may bring in their own kit, or kit may be located in processing department.
o Process and send out kit as instructed.
POWERCHART DOXEPIN (SINEQUAN) LEVEL
NAME
Comment: o Employers each have specific procedures. Certain industries are mandated by DOT
regulations. Chain-of-custody available.
o Refer Healthworks clients to Healthworks at Mercy, Chelsea Creek, 8:00 AM to 5:00
PM. 1-800-622-6352 or 421-5244.
o After hours, Laboratory support services staff will collect the urine specimens.
Clients are to register in patient registration or through ER.
o An Employer representative must accompany the employee and the employee
must have a photo ID. (Exception: Post accident or out of area).
o The Lab will refrigerate the sealed package in a locked box and secure paperwork.
MERCY TEST NAME URINE OVERDOSE PNL MERCY LAB CODE ODDRUG
(d-Amphetamine)
BAR Barbiturates 200ng/mL OXY Oxycodone 100 ng/mL
(Butalbital) (Oxycodone)
(Nordiazepam) (Phencyclidine)
(Buprenorphine) (Norpropoxyphene)
(Benzoylecgonine) (11-nor-9-carboxy-Δ9-THC
MAMP Methamphetamine 500 ng/mL TCA Tricyclic Antidepressants (Desipramine) 300 ng/mL
(d-Methamphetamine)
(Methadone)
Screening test for medical decisions, not for legal chain of custody. Should not be used
for drug compliance testing. Please refer to DRUG ABUSE WITH CHAIN OF
CUSTODY (Regional Lab Clients)
Comment: If urine alcohol is needed, refer to Alcohol Ethyl Urine.
Specimen: 10 ml urine. No preservative.
2 days refrigerated, freeze if > 2 days.
Stability:
Performed: Available stat. Performed at Mercy Laboratory.
TEST NAME DRUG ABUSE WITH CHAIN OF CUSTODY (Regional Lab Clients)
Comment: Regional Lab clients need to order the collection kit directly from MEDTOX. Regional Lab
clients are responsible for the collection process, chain of custody, mailing kit, billing, and
reporting.
MEDTOX Laboratories
402 West County Road D
St. Paul, MN 55112
Phone number: 800-832-3244.
CLIA ID# 24D0665278
MERCY TEST NAME DRUG SCN BLOOD* MERCY LAB CODE DGS
Comments: Detection and identification of prescription or over the counter drugs frequently found in
drug overdose or used with a suicidal intent.
This test is NOT intended for therapeutic drug monitoring or compliance testing.
This test is NOT useful for drugs of abuse or illicit drug testing, including benzodiazepines,
opioids, barbiturates, cocaine, amphetamine type stimulants.
Specimen: 2.75 mL serum from no additive serum tube. Serum from a Serum Separator Tube (SST)
is NOT acceptable.
Centrifuge and aliquot serum into plastic vial within 2 hours of collection.
Processing: Send refrigerated (Preferred) to Mayo. Ambient or frozen is also acceptable. Mayo order
code (DSS).
MERCY TEST NAME OTC/Rx Drug Urine* MERCY LAB CODE OTCU
Comment: This test is limited to prescription and OTC drugs. Drugs of abuse testing will need to be
ordered separately if desired.
This test looks for a broad spectrum of prescription and over-the-counter drugs. It is
designed to detect drugs that have toxic effects. It is intended to help physicians manage an
apparent overdose of an intoxicated patient, to determine if a specific set of symptoms
might be due to the presence of drugs, or to evaluate a patient who might be abusing these
drugs intermittently. This test does not test for all possible drugs.
Specimen: 5 mL random urine in a Sarstedt Aliquot Tube (preferred) or plastic urine container. No
preservative.
Processing: Send refrigerated (preferred) to Mayo. Frozen is also acceptable. Mayo order code (PDSU).
MERCY TEST NAME DRUG SCRN COC QUICK MERCY LAB CODE QDRUG
Comment Refer clients to Healthworks at Mercy, Chelsea Creek, 8:00 AM to 5:00 PM. 1-800-622-6352 or
428-5244.
After hours, Laboratory support services staff will collect the urine specimens using the
chain of custody and perform the Quick Drug screen testing. Employers each have specific
procedures. When Larson Manufacturing employees present to the lab the Quick Drug kit
11+4 is to be used. When Curries/Graham Manufacturing employees present to the lab the
CRLSTAT kit is used. An Employer representative must accompany the employee. The forms
and kits for this testing are kept on site in the draw station room off of the lab waiting room.
Order the test CCDAHW and QDRUG and result as “TCOM” test completed. See specific
procedure for the handling of the paperwork.
CPT Code: 80307
Collect sample on a routine Culturette. Cleanse the external canal. Collect exudate or
Specimen:
scrapings of ear canal.
87070 Culture
CPT Code:
87205 Gram Stain
Includes Anion Gap, Chloride, CO2, Potassium and Sodium. Hemolyzed specimens not
Comment:
acceptable.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: Please see individual test entries
Specimen: 2 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Comment: Useful for the diagnosis of dermatitis herpetiformis and celiac disease and for monitoring
adherence to gluten-free diet in patients with dermatitis herpetiformis and celiac disease.
Note: If Endomysial Antibodies (IgA), Serum is positive or indeterminate, Mayo (EMAT) /Endomysial
(IgA) Titer, Serum will be performed at an additional charge.
Processing: Send refrigerated to Mayo. Ambient or frozen acceptable. Mayo order code (EMA).
Performed: 2-7 days. Test set up at Mayo Monday through Friday; 7 a.m.-5 p.m.
Reference value: Report includes presence and titer of circulating anti-endomysia antibodies. Negative in
normal individuals, also negative in dermatitis herpetiformis or celiac disease patients
adhering to gluten-free diet.
Method: Indirect Immunofluorescence (EMA)
CPT Code: 86231
POWERCHART
ENTEROVIRUS RNA DETECTOR
NAME
MERCY TEST NAME EPSTEIN BARR AB QL (qualitative) MERCY LAB CODE EBVA
Comment: Includes VCA IgG Ab, VCA IgM Ab, EBNA IgG Ab, and Interpretation. Testing is qualitative. If
quantitative test is required, specimen will need to be sent to Mayo.
Specimen: 1 ml serum from a Serum Separator Tube (SST).
Processing: Specimens can be sent refrigerated. Frozen is acceptable. Mayo order code (SEBV)
Cause for
Grossly hemolyzed, lipemic, or icteric samples.
rejection:
Performed: 2-14 days. Monday through Friday; Continuous 9 a.m. – 6 p.m.
Sunday; 6 a.m.
MERCY TEST NAME EBV DNA DET QNT* MERCY LAB EBVQN
CODE
Processing: Send specimen frozen on dry ice only. If shipment will be delayed for more than 24 hours,
freeze plasma at -20 to -80 degrees C (up to 84 days) until shipment on dry ice. Refrigerated
is also acceptable. Mayo order code (EBVQN).
Method
Description: Two site immunoenzymatic (sandwich) assay.
Method
The Atellica IM eE2 assay uses a competitive assay format
Description:
CPT Code: 82670
Order: Indicate which eye when ordering. See beginning of section for ordering help and codes.
Specimen: o Conjunctivitis:
Touch the involved area with a sterile swab moistened with sterile saline. Ideally,
inoculate directly to the appropriate media (Contact Microbiology). However, the
specimen may be transported on a routine Culturette to the lab.
o Corneal scrapings:
The cornea may be anesthetized with 0.5% proparacaine hydrochloride, but better
results are obtained if the scrapings are collected without the use of a topical
anesthetic. A topical anesthetic may have an antimicrobial effect. Scrape the base
and margin of the ulcer. Inoculate these scrapings directly to the appropriate
media.
Comment: o Deliver to Lab immediately. The organisms involved in eye infections are often
fastidious in nature.
o Susceptibility testing will be performed on significant isolates.
o This order is for INTERNAL eye specimens only. If an external eye culture is
needed, order as a wound culture.
RL Client o Write EYE CULTURE on the order form. Indicate which eye was cultured.
Comments: o Send the specimen room temperature to Mercy lab.
Performed: Gram Stain: Within 8 hours of receipt
Preliminary reports: Days 1 & 2
Final report: 3 days
Patient Preparation: A previous bone marrow transplant from an allogenic donor will interfere with testing. Call 800-
533-1710 for instructions for testing patients who have received a bone marrow transplant.
Specimen: 3 mL whole blood in EDTA (Preferred). Yellow top (ACD solution B) and sodium citrate also
acceptable. Invert several times to mix blood.
Processing: Send ambient in original tube. DO NOT ALIQUOT. Mayo order code F5DNA.
Coagulation Consultation Patient Information Sheet must be sent with specimen.
Performed: 3-5 days, Weekly.
Reference Value: Included in Report
Method: Polymerase Chain Reaction/Fluorescence Monitoring
CPT Value: 81241
POWERCHART FACTOR VIII LEVEL
NAME
MERCY TEST NAME COG FAC VIII ASSAY* MERCY LAB CODE F8A
Specimen: o Draw 2 blue top tubes filled appropriately with the amount of blood listed on the
label.
o Avoid gross hemolysis.
o Patient must NOT be receiving coumadin or heparin therapy.
Processing: Double centrifuge specimen, place 1 mL double spun platelet poor plasma into vial. Freeze
specimen immediately. Send FROZEN to Mayo. Mayo code - F8A
Performed: 1-3 Days, performed Monday through Friday
Reference value: Included in report
Method: Activated Partial Thromboplastin Clot-Based Assay
CPT Code: 85240
POWERCHART FACTOR Xa
NAME
Specimen: Draw a blue top tube filled appropriately with amount of blood listed on the label.
Stability: 4 hours refrigerated, freeze if >4 hours.
Comment: Used to monitor dose of Low Molecular Weight Heparin. To monitor unfractionated Heparin
dose, order HEPARIN UNFRAC.
Cause for
Improperly filled tubes will NOT be tested. Gross Hemolysis unacceptable.
Rejection:
Processing: Double spin and freeze plasma if testing not done within 4 hours of collection.
Double spin coagulation specimens to ensure that all platelets are removed: 1. Centrifuge
specimen. Aliquot plasma (leaving some above the cells) to a plastic centrifuge tube. 2.
Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the tube) to
another plastic aliquot tube. 3. Store plasma as required for the test ordered.
o For prevention of venous thromboembolism (VTE) (DVT & PE) a peak Factor X A
drawn 4 hours post SQ injection range is 0.1 - 0.2 U/ml.
o Treatment levels recommended are 0.4 - 1.1 U/ml for twice daily dosing or 1.0 - 2.0
U/ml for once daily dosing.
NOTE: Levels >0.8 - 1.0 U/ml may be associated with increased risk of bleeding.
Specimen: o Draw a blue top tube filled appropriately with the amount of blood listed on the
label.
o Avoid gross hemolysis.
o Patient must NOT be receiving coumadin or heparin therapy.
Processing: Double centrifuge specimen, place 1 mL double spun platelet poor plasma into vial. Freeze
specimen immediately. Send FROZEN to Mayo. Mayo code- (FXCH), Coagulation Factor X
Chromogenic Activity Assay, Plasma.
Performed: 1-3 Days, performed Monday through Friday
Reference Value: Included in report.
Method: Chromogenic
CPT Code: 85260
POWERCHART FAT QUALITATIVE FECES
NAME
MERCY TEST NAME FAT FECES QUALITATIVE MERCY LAB CODE FFQ
Reference Lab Clients: Refrigerate. Specimen must be delivered to lab within 72 hours of
collection.
Performed: As received.
Reference value: Negative. Descriptive report if positive for fat
Method:
Sudan red stain, microscopic examination.
CPT Code: 82705
MERCY TEST NAME FAT FECES QNT* MERCY LAB CODE FTFC
Patient
Patient should be on a controlled diet, 100-150 grams fat per day during collection.
Preparation:
Specimen: o 48- or 72-hour stool specimen collected in a special container obtained from the
Lab.
o 48- or 72-hour specimen preferred, but a 24 hour or random specimen will be
accepted.
o Refrigerate the specimen during and after collection (portable refrigerator
available from the Lab for inpatients).
o 5 grams of stool specimen is required for testing. Continue collection until 5 grams
collected.
Comment: o Must indicate length of collection period in comment.
o Barium in the stool will interfere with the test.
o It is essential that laxatives (particularly mineral oil and castor oil) are NOT used
during the collection period.
o Synthetic fat substitutes such as Olestra interfere with test procedure.
o Wait a minimum of 48 hours following a barium procedure before beginning
specimen collection.
Processing: o Send entire specimen in container that is no more than three-fourths full.
o Indicate length of collection period.
o Send frozen to Mayo. Mayo order code FATF.
Performed: 3 days. Test set up Monday through Friday.
Reference Value: Included with report.
Method: Nuclear Magnetic Resonance Spectroscopy (NMR).
CPT Code: 82710
Comment: o To be ordered on MISYS when a test result is to be faxed or called to a location in
addition to the normal reporting location.
Include the fax telephone number, mailing address, to whom the report should be directed,
and for which tests.
POWERCHART FEBRILE AGGLUTININ
NAME
Specimen: 1 ml serum from a no additive serum tube (Preferred). Also acceptable Serum Separator
Tube (SST).
Draw blood immediately before next scheduled dose. Centrifuge and aliquot serum into
plastic vial within 2 hours of collection.
Processing: Send refrigerated (Preferred) to Mayo. Ambient or frozen also acceptable. Mayo code
(FELBA).
Serum ferritin values are elevated in the presence of the following conditions and do not
reflect actual body iron stores:
– inflammation
– significant tissue destruction
– liver disease
– malignancies such as acute leukemia and Hodgkin’s disease
– therapy with iron supplements
Method The Atellica IM Fer assay is a 2-site sandwich immunoassay using direct chemiluminescent
Description: technology, which uses constant amounts of 2 anti-ferritin antibodies.
CPT Code: 82728
MERCY TEST NAME FERTILITY TEST SEMEN MERCY LAB CODE SMNFER
Specimen: Specimen Collection Kit may be obtained from the Lab. This kit is the only acceptable
collection system available.
Specimen: o Draw 1 blue top tube filled appropriately with amount of blood listed on label.
o 1 ml plasma needed.
o Avoid hemolysis.
Other: Improperly filled tubes will NOT be tested.
Processing: o Centrifuge and separate plasma within 2 hours.
o Store in refrigerator up to 4 hours.
o Double spin and freeze plasma if storage will be longer than 4 hours.
o Label vial "CITRATED PLASMA".
Double spin coagulation specimens to ensure that all platelets are removed: 1. Centrifuge
specimen. Aliquot plasma (leaving some above the cells) to a plastic centrifuge tube. 2.
Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the tube) to
another plastic aliquot tube. 3. Store plasma as required for the test ordered.
POWERCHART
LIVER FIBROSIS FIBRO TEST ACTITEST PANEL
NAME
Specimen: 3 mL serum from a Serum Separator Tube, no additive serum tube acceptable.
Centrifuge and aliquot serum into an amber vial within 2 hours of collection. It must
be protected from light.
Cause for
Rejection: Specimen not protected from light.
Processing: Centrifuge and aliquot serum into an amber vial or wrap the aliquot tube in foil within 2
hours of collection. Send refrigerated. Mayo (FIBRO).
Performed:
2 days, HAPTF, A2MF: Monday through Saturday
ALTF, GGTF, TBILF: Monday through Sunday
APOAF: Monday through Saturday
Reference Value:
Included in Report
Method:
INTF: Algorithm and Interpretation provided through BioPredictive
APOAF: Automated Turbidimetric Immunoassay
A2MF, HAPTF: Nephelometry
ALTF:Photometric Rate, L-Alanine with Pyridoxal-5-Phosphate
GGTG:Photometric Rate
TBILF:Photometric, Diazonium Salt (DPD)
81596
CPT Code:
Methotrexate and leucovorin interfere with folate measurement because these drugs cross-
react with folate binding proteins.
Performed:
Within 8 hours of receipt. Available stat.
TEST NAME FOLIC ACID RBC / FOLATE RBC See: FOLATE SERUM
True folate deficiency in the current era of FDA-mandated folic acid supplementation is
exceedingly rare. There is no evidence to support routine ordering of RBC or serum folate,
but serum folate concentrations provide equivalent clinical information to RBC folate in the
assessment and diagnosis of folate deficiency. Based on these statistics, and because serum
folate provides equivocal results to RBC folate in almost all clinical scenarios, routine
ordering of RBC folate is no longer warranted.
Specimen: Random urine specimen plus 1 ml serum. Blood specimen must be collected within 1 hour
of urine specimen.
Stability: Sodium in serum and urine may be stored for up to 7 days at 2–8°C or stored frozen for up
to 30 days at -20°C.
Comment: Includes random urine sodium, random urine creatinine, and Fractional Excretion Sodium
Interpretation.
Lab will place an order for a SERUM Sodium and SERUM Creatinine when the urine
specimen is received in the Lab.
Performed: Within 8 hours of receipt. Available Stat.
Reference Range: Interpretation table is included with results. Calculations are based on Urine Sodium, Urine
Creatinine, Serum Sodium and Serum Creatinine.
Method Refer to individual test entry
Description:
CPT Code: 84300 Sodium Ur+
82570 Creat R UR
POWERCHART
CHROMOSOME STUDY FRAGILE X
NAME
FRAG X MOL ANLYS* FXMA
MERCY TEST NAME MERCY LAB CODE
Specimen: 2.5 ml EDTA whole blood from purple top tubes or a yellow ACD tube. Minimum 1 ml. Draw
as much as possible, as Mayo preserves some for more testing, and also for repeat testing.
NOTE: Amniotic fluid and chorionic villus may also be tested. DO NOT collect these
specimens before consultation with Mayo Medical Laboratories. Complete collection
instructions are found in the Mayo catalog. Call the Lab for a copy of these instructions.
Comment: Useful for documentation of carrier status and prenatal diagnosis for fragile X syndrome.
Prior consultation with a medical geneticist is recommended.
MERCY TEST NAME FFP FOR INFUS MERCY LAB CODE TFFP
Specimen: EDTA plasma in a pink or purple top tube. MRN must be check marked.
Use: Usage is indicated in the treatment of clotting factor deficiencies.
Comment: o Use one order for up to 6 units.
o In Powerchart, if plasma is needed "STAT", in the "Transfusion Priority" drop
down select "STAT".
o In Powerchart, if plasma is needed for a FUTURE DATE and TIME, in the
"Transfusion Priority" drop down select "TIMED" and select future date and time to
when you want the infusion.
o If blood type has not been ordered for the episode, order "ABO+Rh(D) Blood
Typing."
o Indicate number of units in the units ordered field.
o Allow 6 minutes thawing time for each unit ordered of FFP.
o If FFP is for routine use, the process to receive a unit is to send the "Blood Product
Request" slip when the unit is ready to be transfused. When the request form is
received the product will be thawed and the blood bank will call the requesting
location to tell them the product is ready to be picked up.
o During Massive Transfusion or Emergency Release, units will automatically be
thawed according to orders.
Processing: Give group specific or compatible disregarding Rh. Refer to procedure if specific group is
unavailable.
Reference Lab Clients: Fresh tissue specimen (no formalin) must be kept on ice and
transported to Mercy Histology Lab immediately. Notify the Histology Lab (641-428-7486)
that the specimen is coming.
Performed: Pathologist report will be called to the physician within 15 minutes of receipt.
Performed: Daily
Method The Atellica IM FSH assay is a 2-site sandwich immunoassay using direct chemiluminescent
Description: technology, which uses constant amounts of 2 antibodies that have specificity for the
intact FSH molecule.
CPT Code: 83001
RL Client
o Write FUNGUS CULTURE on order form. Indicate specimen source.
Comments:
o Send specimens at room temperature to Mercy lab.
Performed: Direct preparation: 1 day
Preliminary report: 2,3 weeks
Final report: 4 weeks
Specimen: Submit each yeast or fungus to be identified on a separate plate. 1 yeast or fungus per
request.
RL Client o Write FUNGAL IDENTFICATION on the order form. Indicate the source of the
Comments: specimen.
o Send the culture plates sealed and at room temperature to Mercy lab.
FUNGAL SURVEY, FUNGAL ANTIBODY PANEL - Discontinued 05/06/14 by Mayo Laboratories. Please refer to
the April 2014 Lab Links for more details. The following tests can be ordered individually.
o Histoplasma Antibody Screen, Mercy code HSTAB
o Blastomyces Antibody, Mayo code BLAST
o Cryptococcus Antigen, Mayo code SLFA
o Coccidioides Antibody, Mayo code COXIS
Specimen: 6.0 ml whole blood drawn in EDTA tube or yellow top (ACD solution A) tube.
Do not spin down.
Processing: Send refrigerated to Mayo. Mayo order code (G6PD1).
Do not transfer blood to other containers/send in original tube.
Do not allow specimen to freeze.
Use bubble wrap to protect specimen.
Performed: Test set up at Mayo Monday through Sunday.
Reference value: Included with the report.
Method: Kinetic Spectrophotometry (KS)
CPT Code: 82955
POWERCHART GALECTIN 3 LEVEL
NAME
Male:<73 units/L
Method The Atellica CH Gamma-Glutamyl Transferase (GGT) assay is based on the reaction with
Description: synthetic substrate (L-γ-glutamyl-3-carboxy-4-nitroanilide), glycylglycine acts as an acceptor
for the γ-glutamyl residue and 5-Amino-2-Nitrobenzoate (ANB) is liberated.
CPT Code: 82977
Patient
Patient must be fasting.
preparation:
Specimen: 1 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send frozen to Mayo. Mayo order code GAST.
Performed: 3 days. Test set up Monday through Friday;5 a.m.-12 a.m., Saturday; 6a.m.-6 p.m.
Reference value: Included in report
Method: Automated Chemiluminescent Immunometric Assay.
CPT Code: 82941
MERCY TEST NAME GASTROCCULT® BODY FLD MERCY LAB CODE GASO
MERCY TEST NAME GASTROINTESTINAL PATHOGEN PANEL PCR, FECES MERCY LAB CODE CMIS
Specimen: Fresh stool placed in an orange-topped Cary Blair stool preservative. Enough stool should be
added to the preservative vial to bring the level of the preservative fluid up to the red line,
on the preservative container.
Specimen: Urine: First void urine specimens must be transferred from the collection cup to the Xpert
CT/NG Urine Transport Reagent Tube (Yellow Cap) immediately (preferred) or within 24
hours of collection when kept at Room Temperature or within 8 days of collection when
stored at 2–8°C.
Urine specimens in Xpert CT/NG Urine Transport Reagent Tubes should be kept between 2°C
and 30°C during transport and can be stored for up to 3 days at this temperature.
Cause for
Rejections o Improperly collected samples.
Comment: In the case of suspected child abuse, culture is the only recommended procedure. See: GC
Culture.
Specimen: Swab specimen collected using the APTIMA Collection Vaginal Swab (the APTIMA Unisex
Swab can also be used). Collection kits are available from Mercy lab.
Mayo Approved The following sites are approved for GC testing at Mayo Med Labs, ONLY (Mercy Lab is not
Sites: approved to do testing on these sites
o Rectal/anal
o Ocular (corneal/conjunctiva)
o Oral/throat
o Pelvic wash, cul-de-sac fluid (this source requires the APTIMA specimen transfer
tube T652, available from Mercy lab).
NOTE: If provider wants both Chlamydia and GC testing done on a rectal, ocular, oral
or pelvic, a separate order will have to be placed for each test.
Comment: o In the case of suspected child abuse, culture is the only recommended procedure.
See GC culture.
RL Client o If ordering the test at your facility, order a CMIS and put in comment the test is for
Comments: MGRNA and include the source (rectal, ocular, oral). If you will order using a
requisition, write CMIS on the order form and indicate the testing is for MGRNA
and include the source (rectal, ocular, oral).
o Send the APTIMA transporter refrigerated to Mercy lab.
Cause for o Transport tubes that are received without collection swabs inside.
rejection: o Transport tubes that have expired.
o Transport tubes received with a swab different from the one provided in the
collection kit.
o Sources other than those listed above.
Processing: Refrigerate sample after collection and send to Mayo Med Labs refrigerated. Mayo order
code MGRNA (N. gonorr, Misc., Amplified RNA)
MERCY TEST NAME GENERAL HEALTH PANEL MERCY LAB CODE GHP
MERCY TEST NAME GENITAL LOW CLT MERCY LAB CODE GENL
Comment: o This culture screens for the presence of Group B Beta Streptococcus,
Staphylococcus aureus, Gardnerella vaginalis, and a predominance of yeast.
o Susceptibility testing will routinely be performed on significant isolates of
Staphylococcus aureus.
RL Comments: Write Genital Tract Lower Culture on RL order form. Indicate Collection site. Send specimen
at Room temp.
MERCY TEST NAME eGFR estimated Glomerular Filtration Rate MERCY LAB
CODE
Stability: Separated serum and plasma specimens may be stored for up to 2 days at 2–8°C or stored
frozen at or below -20°C.
Comment: eGFR is a calculation and not orderable by itself. eGFR is reported with every creatinine test
ordered.
Performed: Within 8 hours of receipt. Available Stat
Reference Range: ≥ 60 mL/min/1.73m2
eGFR will not calculate if the patient is under age 18 or if the patient sex is not specified as male or female.
Method: The Laboratory is using the Chronic Kidney Disease Epidemiology Collaboration (2021 CKD-EPI)
eGFRcr = 142 x min (Scr/κ, 1) α x max (Scr/κ, 1)-1.200 x 0.9938Age x 1.012 [if female]
Age (years)
CPT Code: NA
Specimen: 2 grams fresh feces. Collect sample in a container with a tight-fitting lid. Deliver to Mercy lab
as soon after collection as possible. Testing needs to occur within 72 hours of collection.
Transport refrigerated.
Fresh stool specimens can also be frozen, after collection, if testing cannot be performed
within 72 hours of collection. Frozen samples are stable for 90 days. Transport frozen.
This method can also be tested with the ParaPak C&S (orange lid) preservative. Testing
needs to occur within 72 hours of collection. Transport at room temperature if a stool
culture is also ordered on this same C&S vial.
Cause for
o Specimens collected within 7 days of barium or bismuth enema are not acceptable.
rejection:
o Specimens should not be contaminated with toilet water or urine.
Comment: Detects Giardia and Cryptosporidium antigens. Tests are not available separately.
RL Comments:
o Mark GIARDIA/CRYP RAPID STOOL on the order form.
Performed: Daily 1500 cutoff.
*Not more than one specimen in 24 hr. period.
MERCY TEST NAME G BASE MEMBRAN IGG* MERCY LAB CODE GBM
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Cause for
Hemolysis, Lipemia
Rejection:
Processing: Send refrigerated to Mayo. Frozen acceptable. Mayo order code GBM.
Performed: Monday – Friday, Sunday;11a.m.
Method: Multiplex flow immunoassay
CPT Code: 83516
POWERCHART GLUCOSE LEVEL
NAME
Method
The Atellica CH Glucose Hexokinase_3 (GluH_3) assay uses a two-component reagent
Description:
CPT Code: 82947
MERCY TEST NAME GLUCOSE (GLUCOSE DONE BY LAB ON WHOLE BLOOD) MERCY LAB CODE GLUCR
Method
The Atellica CH Glucose Hexokinase_3 (GluH_3) assay uses a two-component reagent.
Description:
CPT Code: 82945
Comment: Perform between 24- and 28-weeks’ gestation on all pregnant women not identified as
having glucose intolerance. Screening is performed without regard to the time of day or last
meal.
Test available:
Performed: Outpatient Drawing/Core Lab – Monday-Friday 0800-1700
Core Lab – Saturday and Sunday 0800 - -1200
MERCY TEST GLUC TOL 2HR (ORDER FASTING, 1 HOUR AND 2 MERCY LAB CODE GLUC0T
NAME HOUR TESTS) (FASTING)
GLUC1T (1
HOUR)
GLUC2T (2
HOUR)
Patient preparation:
o Patient should have a regular diet with adequate carbohydrates for three days
before test.
Excessive amounts of sugars should be avoided. Reducing diets are not satisfactory.
o Patient should maintain normal activities with no excessive vigorous exercise.
o Patient should not be acutely ill. Test should not be performed during acute medical
or surgical stress and not for several months after an acute myocardial infarction.
o Patient should be fasting for at least 8 hours. Moderate amounts of water are
permissible.
o Discontinue medications as directed by physician.
o Patient should remain seated and should not smoke throughout the test.
o No other tests or procedures should be scheduled during a Glucose Tolerance Test.
Outpatients having tolerance testing in the Laboratory should be prepared to stay
in the Laboratory waiting area for the duration of the test.
Pediatric patients: The amount of glucose given to pediatric patients is by weight for
patients 25-95 lbs. See Policy in PolicyStat for details.
Method
The Atellica CH Glucose Hexokinase_3 (GluH_3) assay uses a two-component reagent
Description
CPT Code: 82951
GLUC1T
(1 HOUR)
GLUC2T
(2 HOUR)
GLUC3T
(3 HOUR)
Patient preparation:
o Patient should have a regular diet with adequate carbohydrates for three days before test.
Excessive amounts of sugars should be avoided. Reducing diets are not satisfactory.
o Patient should maintain normal activities with no excessive vigorous exercise.
o Patient should not be acutely ill. Test should not be performed during acute medical or
surgical stress and not for several months after an acute myocardial infarction.
o Patient should be fasting for at least 8 hours. Moderate amounts of water are permissible.
o Discontinue medications as directed by physician.
o Patient should remain seated and should not smoke throughout the test.
o No other tests or procedures should be scheduled during a Glucose Tolerance Test.
Outpatients having tolerance testing in the Laboratory should be prepared to stay in the
Laboratory waiting area for the duration of the test.
Pediatric patients: The amount of glucose given to pediatric patients is by weight for
patients 25-95 lbs. See Policy in PolicyStat for details.
Stability Urine should be stored at 4°C during collection. Urine samples may lose as much as 40% of
their glucose after 24 hours at room temperature.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: No established reference range available
Method
Description: The Atellica CH Glucose Hexokinase_3 (GluH_3) assay uses a two-component reagent.
CPT Code: 82945
MERCY TEST NAME Misc. General Lab (CMIS) Designate: test name GLUCOSE MERCY LAB CODE CMIS
RANDOM URINE in comment
POWERCHART GLUTAMIC ACID DECARBOXYLASE AUTOANTIBODIES
NAME
Test included: This included testing for Glutamic Acid Decarboxylase Autoantibodies, Mayo order (GD65S)
NOTE* If physician ordered both Glutamic Acid Decarboxylase Autoantibodies (Mayo GD65S)
and Islet Antigen 2 Antibody (Mayo IA2) See: I2GAD
Specimen: 1.0 ml serum from a no additive serum tube or a Serum Separator Tube (SST).
Processing: Send refrigerated to Mayo. Mayo order code (GD65S) Frozen acceptable. Ambient
Performed: 2-6 days, Sunday thru Thursday; 10 PM
Reference value: Included with results.
Method: Radioimmunoassay (RIA)
CPT Code: 86341
MERCY TEST NAME GRAM STAIN DIRECT MERCY LAB CODE GRAM
RL: Next day, 1st shift unless ordered STAT with a specific phone number indicated
MERCY TEST NAME GRP B STREP CLT MERCY LAB CODE GBOB
Order: Place only 1 order for Culturette (sites). Specify the source(s) when ordering (i.e.: vag-rect).
MERCY TEST NAME GRP B STREP CLT/SENS MERCY LAB CODE GBOBS
Order: Place only 1 order for Culturette (sites). Specify the source(s) when ordering (i.e.: vag-rect).
Patient
Patient must be fasting. (Overnight – 8 hours)
preparation:
Specimen: 0.6 ml serum from a SST or plain red top tube. Minimum 0.5 ml.
Processing: Send refrigerated to Mayo. Frozen acceptable. Mayo order code HGH.
Performed: 1- 3 days. Monday through Friday 5 a.m. - 12 a.m., Saturday 6 a.m.- 6 p.m.
Reference value: Included in report.
Method: Immunoenzymatic immunoassay
CPT Code: 83003
POWERCHART HAPTOGLOBIN
NAME
Method The Atellica CH Haptoglobin (Hapt) assay is based upon the reaction between antibody and
Description: haptoglobin in a serum sample.
MERCY TEST NAME HCG QUANT SERUM MERCY LAB CODE HCGQ
This test may be used for detecting pregnancy by the first day of the missed menstrual
period.
All in vitro assays can generate erroneous results, both clinically false positive results (test
results suggesting a condition that is absent) and clinically false negative results (test results
failing to identify a condition that is present).
Performed: Within 8 hours of receipt. Available Stat.
Gestational age:
1 week: 5 - 50 IU/L
1-2 weeks: 50 - 500 IU/L
2-3 weeks: 100 - 5,000 IU/L
3-4 weeks: 500 - 10,000 IU/L
4-5 weeks: 1,000 - 50,000 IU/L
5-6 weeks: 10,000 - 100,000 IU/L
6-8 weeks: 15,000 - 200,000 IU/L
8-12 weeks: 10,000 - 100,000 IU/L
3rd trimester: 5,000 - 50,000 IU/L
During the first six weeks of pregnancy, serum HCG concentrations have a doubling time of
approximately 2 days. A maximum is reached by the second to third month and followed by
a decrease to as low as 5000 by the third trimester (6-9 months). Following delivery, HCG
concentrations rapidly decrease and usually return to normal within several days post-
partum.
Method The Atellica IM ThCG assay is a 2-site sandwich immunoassay using direct chemiluminescent
Description: technology, which uses constant amounts of 2 antibodies.
Specimen: 1 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Note: Patient Preparation: For 12 hours before specimen collection, do not take multivitamins or
dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin,
and nail supplements and multivitamins.
Processing: Send to Mayo refrigerated. Frozen acceptable. Mayo order code (BHCG).
Performed: 1-3 days. Test set up Monday through Saturday Varies
Method: Electrochemiluminescence Immunoassay
CPT Code: 84702
MERCY TEST NAME HDL CHOL (High Density Lioprotein) MERCY LAB CODE HDL
Low: <40mg/dl
Acceptable: 40-59 mg/dl
Optimal: ≥60 mg/dl
Method The Atellica® CH HDL Cholesterol (HDLC) assay is a two-reagent format and depends on the
Description: Accelerator Selective Detergent methodology.
CPT Code: 83718
POWERCHART HELICOBACTER PYLORI FECES / H. PYLORI FECES
NAME
Performed: 1 week
POWERCHART HEMATOCRIT
NAME
MERCY TEST NAME HFE GENE ANALYSIS* MERCY LAB CODE HHEMO
Specimen: 2.5 mL whole blood in lavender top (EDTA) or yellow top (ACD).
Processing: Send ambient to Mayo. Mayo order code (HFET)
Performed: 6 - 7 days. Monday through Friday
Reference value: Included with test results
Method: Droplet Digital Polymerase Chain Reaction (ddPCR)
CPT Code: 81256
POWERCHART HEMOGLOBIN
NAME
MERCY TEST NAME HGB POST DIALYSIS MERCY LAB CODE HGBXPD
Specimen: 0. 5 ml EDTA whole blood from purple top tube or 1 full purple capillary tube.
Stability: Specimens may be stored for up to 48 hours at room temperature,8 for up to 7 days at 2–
8°C, or stored frozen for up to 21 months (with one freeze-thaw) at -70°C.
Also included is a calculated mean blood glucose.
Comment:
Within 8 hours of receipt. Available stat.
Performed:
Reference Range: 4.0-5.6%
Method The Atellica CH A1c_E assay consists of two separate measurements: glycated hemoglobin
Description: (A1c_E) and total hemoglobin (tHb_E). The two measurements are used to determine the
%HbA1c (NGSP units) or the hemoglobin A1c_E/tHb_E ratio in mmol/mol (IFCC units). The
individual concentration values of A1c_E and tHb_E generated by this assay are used only for
calculating the %HbA1c or A1c_E/tHb_E ratio.
CPT Code: 83036
Specimen: 10 ml EDTA whole blood from a EDTA tube. ACD (solution B) or sodium heparin is also
acceptable.
Comment: Include recent transfusion information.
The laboratory has extensive experience in hemoglobin variant identification and many
cases can be confidently classified without molecular testing. However, molecular
confirmation is always available, subject to sufficient sample quantity (e.g., multiplex ligation-
dependent probe amplification testing requires at least 2 mLs of sample in addition to
protein testing requirements). If no molecular testing or specific molecular tests are desired,
utilize the appropriate check boxes on the form. If the form or other communication is not
received, the reviewing hematopathologist will select appropriate tests to sufficiently explain
the protein findings which may or may not include molecular testing.
MERCY TEST NAME HEMOGLBN S SCN* (Sickle Cell) MERCY LAB CODE HGBS
MERCY TEST NAME HEMOGRAM PLATELET CT MERCY LAB CODE See: CBC
Patient o Patient should be instructed to refrain from red meat and aspirin for 3 days prior
preparation: to specimen collection.
o IMPORTANT: Note on order whether patient has complied with instructions.
Specimen: o 1 gram of feces from a single defecation is to be collected using a spoon-like
sampler from the kit supplied by the Laboratory.
o Place sample in screw-capped tube.
Processing: o Send refrigerated to Mayo.
o Mayo code order code (HQ).
Performed: Test set up Monday through Saturday
Reference value: Included in report
Method: Fluorescence Quantitation.
CPT Code: 84126
Specimen: Draw a blue top tube filled appropriately with amount of blood listed on label.
Stability: 8 hours refrigerated, double spin and freeze if >8 hours.
Comment: o Used to monitor dose of unfractionated Heparin.
o To monitor low molecular weight heparin, order Factor X A.
Cause for o Improperly filled tubes will NOT be tested.
rejection: o Gross hemolysis unacceptable.
Processing: o Centrifuge within 30 minutes.
o Separate plasma within 2 hours and analyze within 8 hours.
o Double spin and freeze plasma if testing not done within 8 hours of collection.
o Label vial "Citrated Plasma".
Double spin coagulation specimens to ensure that all platelets are removed: 1. Centrifuge
specimen. Aliquot plasma (leaving some above the cells) to a plastic centrifuge tube. 2.
Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the tube) to
another plastic aliquot tube. 3. Store plasma as required for the test ordered.
Performed: Available stat. Performed within 8 hours of receipt except for special studies to establish
therapeutic PTT ranges.
Reference value: 0.3-0.7 u/ml
Method: Chromogenic Substrate
CPT Code: 85520
Specimen: o 0.5 mls plasma from a properly filled blue top tube.
o Remove plasma by a double spin method and freeze if testing will not be
performed within 2 hours of draw time.
o
Performed: Same shift, Sunday through Saturday. Available stat.
Reference value: Negative
Method: Particle ImmunoFiltration Assay (PIFA)
CPT Code: 86022
MERCY TEST NAME HEPATIC (Liver) FUNCTION PNL MERCY LAB HFPL
CODE
Stability: Specimens may be stored for up to 8 hours at 25°C or for up to 7 days at 2–8°C or stored
frozen for up to 6 months at -20°C or colder.
Comment: Includes: Albumin, Alkaline Phosphatase, ALT, AST, Total Bilirubin, Direct Bilirubin, Indirect
Bilirubin, Total Protein, and A/G Ratio
Performed: Within 8 hours of receipt. Available stat.
Reference value: See individual test entry
Method: See individual test entry
CPT Code: 80076
POWERCHART HEPATITIS A IgM ANTIBODY
NAME
Specimen: 1 ml serum from a Serum Separator Tube (SST). Centrifuge within 2 hours of collection.
Stability: 8 hours room temp, or 48 hours refrigerated. Freeze if >48 hours.
Included in: Hepatitis Acute Panel or may be ordered separately.
Cause for The following samples are unacceptable and will not be tested; heat treated, hemolyzed,
rejection: cadaveric specimens, body fluid other than serum or plasma.
Reference value: Non-Reactive. Reactive specimens will have Hepatitis A IgM Antibody (HAVMAB)performed at
an additional charge.
MERCY TEST NAME HEP A IGG AB* MERCY LAB CODE HAIGG
Specimen: 1.0 mL serum from a Serum Separator Tube (SST). Centrifuge within 2 hours of collection
and aliquot into plastic vial.
Includes: Hepatitis B Surface Antigen, Hepatitis B Core IgM Antibody, Hepatitis C Antibody, Hepatitis A
Antibody IgM
86706Includes: Hepatitis B Surface Antigen, Hepatitis B Surface Antibody, Hepatitis C Antibody, Hepatitis B
Core Total Antibody
MERCY TEST NAME HEPATITIS CHRONIC ACUTE MERCY LAB CODE HPCHAC
Includes: Hepatitis B Surface Antigen, Hepatitis B Core IgM Antibody, Hepatitis C Antibody, Hepatitis A
Antibody IgM, Hepatitis B Surface Antibody, Hepatitis B Core Total Antibody
MERCY TEST NAME Hepatitis B Core Total Antibody MERCY LAB CODE HBCTAB
Comment: If Hepatitis B Core Total Antibody is detected, Hepatitis B Core, IgM Antibody will be
performed and charged.
Method: Chemiluminescent Microparticle Immunoassay
CPT Code: 86704- HBCTAB
86705-HBCMAB (if appropriate)
Specimen: 1.5 mL serum from a Serum Separator Tube (SST). Centrifuge within 2 hours of collection
and aliquot into plastic vial.
86706 Includes: Hepatitis B Surface Antigen, Hepatitis B Surface Antibody, Hepatitis C Antibody, Hepatitis B
Core Total Antibody
Cause for The following samples are unacceptable and will not be tested; heat treated,
rejection: hemolyzed, heparinized, cadaveric samples, body fluids other than serum or plasma.
Comments: o If Hepatitis B Surface Antigen is detected, specimen will be forwarded to Mayo
Medical Laboratories for additional testing. Mayo code - HBAG, HBGNT if
appropriate.
o Performance has not been established for newborns, cord blood, body fluids.
MERCY TEST NAME HEP B CORE IgM AB MERCY LAB CODE HBCMAB
MERCY TEST HEPATITIS C AB HIGH RISK (used only for Medicare MERCY LAB CODE HCVABR
NAME screening)
MERCY TEST NAME HCV RNA DETECT/QN* MERCY LAB CODE HCVRNA
Processing: o Spin down. **Remove serum from cells within 2 hours of collection. Freeze
aliquoted serum immediately for transport.
o Send frozen to Mayo. Mayo code - HCVQN.
Performed: 1-3 days. Monday through Saturday; 7 a.m. - 4 p.m.
Reference Value: Included with results.
Method: Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
CPT Code: 87522
Specimen: 5.0 mL serum from a Serum Separator Tube (SST) or no additive serum tube. Centrifuge
within 2 hours of collection and aliquot into plastic vial.
Note: 1. Specimens should contain a recommended minimum hepatitis C virus (HCV) viral load of
500 IU/mL.
Specimen: Must indicate specimen source. Submit only 1 of the following specimens:
Body fluid (Pleural, peritoneal, ascites, pericardial, amniotic, or ocular) – 0.5 mL of fluid in a
sterile container. Do not Centrifuge. Send to Mayo in sterile container.
Swab (Genital, dermal, ocular, nasal, throat, or oral) - Culturette (BBL Culture Swab)
(T092). Send swab to Mayo in multimicrobe media (M4-RT, M4 or M5).
Respiratory (Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing,
sputum, or tracheal aspirate) – 1.5 mL of specimen in a sterile container. Send to Mayo in sterile
container.
Tissue (Brain, colon, kidney, liver, lung, etc.) - Whole collection in a Multimicrobe media (M4-RT)
(T605) (Preferred) or Sterile container with 1–2 mL of sterile saline. Submit only fresh tissue.
Urine (<1-month old infant) – 0.5 mL of urine in a sterile container.
Cautions: A negative result does not eliminate the possibility of herpes simplex virus (HSV) infection.
Although the reference range is typically “negative” for this assay, this assay may detect viral
nucleic acid shedding in asymptomatic individuals. This may be especially relevant when dermal
or genital sites are tested since intermittent shedding without noticeable lesions has been
described.
This assay is only to be used for patients with a clinical history and symptoms consistent with
HSV infection and must be interpreted in the context of the clinical picture.
Processing: Send refrigerated (preferred) to Mayo. Frozen is also acceptable. Mayo order code (HSVPV).
Performed: 1-4 days. Monday through Saturday.
Reference value: Reference ranges included with report.
Method: Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
CPT Code: 87529 x 2
MERCY TEST NAME HERPES SIMPLEX PCR CSF* MERCY LAB CODE HSVC
Specimen: 0.2 mL Spinal Fluid in a sterile container (12 x 75 mm screw cap vial)
Processing: Specimen should be aliquoted and sent refrigerated. Mayo order code HSVC
MERCY TEST NAME HSV TYPES 1 & 2 AB* MERCY LAB CODE HSVT12
Specimen:
1.0 ml serum from a no additive serum tube or a Serum Separator Tube (SST).
Processing: Specimen should be aliquoted and sent refrigerated. Mayo order code (HSVG).
TEST NAME HERPES SKIN SCRAPING FOR See: Cytology Section Tzanck Smear
CYTOLOGY
POWERCHART HIAA-5 HYDROXYINDOLE ACETIC ACID URINE
NAME
Patient For 48 hours before starting the 24-hour collection and during the collection do not eat any
preparation: of the following: avocados, bananas, butternut, cantaloupe, dates, eggplant, hickory nut,
grapefruit, honeydew melon, kiwi fruit, nuts, pecans, pineapples, plantain, plums, tomatoes,
tomato products and walnuts.
Specimen: 0.7 ml serum from a Serum Separator Tube (SST) or a no additive serum tube
Processing: Send to Mayo refrigerated. Frozen is acceptable. Mayo order code (SHSTO).
Performed: 1 day. Monday through Friday 930 AM.
Reference value: Included in report.
Method: Complement Fixation (CF)/ Immunodiffusion
CPT Code: 86698x3
Specimen: 4 mL urine from random urine collection in plastic 5 mL aliquot tube. No preservative.
Specimen: 1.5 ml serum from a Serum Separator Tube (SST) for initial testing done at Mercy.
Stability: Serum: 24 hours room temp, 14 days refrigerated.
Cause for Cord blood is not an acceptable specimen for HIV testing. The mother's serum should be
rejection: tested.
Comment: If this initial HIV testing is reactive, then laboratory will place an additional order for
(Sunquest HIVDI / Mayo HIVDI) - HIV 1 HIV 2 Ab Differentiation confirmation testing, which is
performed at Mayo Medical Laboratories at an additional charge. Additional testing may be
performed and charged based on the HIVDI result.
MERCY TEST NAME HIV 1 DETECT QUANT* (HIV Viral Load) MERCY LAB CODE HIVDQ
Specimen: Requires 2 purple top EDTA tubes. This test requires 1.5 mL of plasma from EDTA whole
blood collection. 1 EDTA tube may not provide enough plasma.
Spin down and remove plasma within 6 hours of collection. Send FROZEN plasma specimen
Processing:
to Mayo (refrigerated is acceptable) Mayo code - (HIVQN)
Performed: Monday - Thursday 1 day, Friday and Saturday, 3 days. Monday - Saturday testing performed
7 a.m. - 4 p.m.
Reference value: Included in report.
Method: Real Time Reverse Transcription - Polymerase Chain Reaction (RT-PCR)
CPT Code: 87536
Processing: Aliquot 1.5 mL serum to plastic vial, send FROZEN to Mayo Medical Laboratories. Mayo Code
– HIVDI.
Comment: This test is reflexed from our in-house HIV testing. If the initial HIV testing is reactive lab will
send to Mayo for this HIVDI confirmation/differentiation testing at an additional charge.
Mayo may perform additional testing beyond the HIVDI at an additional charge.
This test is also available to order if patient is previously identified as HIV positive and
additional testing is desired to follow patient's condition.
This test is designed to be done on the SOURCE patient only, following an exposure
(blood and/or body fluids). The source patient is not required to sign an informed
consent when an exposure has occurred but should be informed that testing will be
taking place. Orders need to be sent on a manual form.
Cause for Cord blood is not an acceptable specimen for HIV testing. The mother's serum should be
rejection: tested.
Comments: o If employee exposure testing is needed, order a routine HIV. The routine
HIV test will be done and tests for HIV1 and HIV2 antibodies and HIV1 p24
Antigen.
o To be ordered by Mercy Employee Health, Nursing Supervisor or any outside
location requiring source patient testing.
o Reports are hand delivered to in-house Nursing Supervisors, or ED
providers. Results are faxed to Employee Health and ASC. Results do not go
to Powerchart.
o If this initial HIV testing is reactive, then laboratory will place an additional
order for HIVDI- HIV 1 HIV 2 Ab Differentiation confirmation testing, which is
performed at Mayo Medical Laboratories at an additional charge.
o If the primary instrument in Lab is down, the rapid Alere Determine HIV-1/2
Ag/Ab Combo kit will be performed on the source patient only.
Reference value: Non-Reactive
Method: Chemiluminescent Microparticle Immunoassay
CPT Code: 87389-HIV-1 and HIV-2 Antibody, HIV 1 p24 Antigen single assay.
Processing: o Submit in original lavender top tubes, do not transfer blood to other containers.
o Send Ambient Do NOT refrigerate and clearly label "DO NOT REFRIGERATE."
Mayo order code (LY27B).
Performed: 2-6 days. Monday through Friday 730 AM and 500 PM.
Reference value: Included with test results
Method: Flow cytometry. All positive test results will be confirmed by complement dependent
cytotoxicity (CDC).
CPT Code: 86812
Stability: Centrifuge samples and remove serum or plasma from red blood cells as soon as possible.
to ensure accurate measurement. Samples that cannot be separated soon after collection
should be stored on ice until centrifugation. Do not store samples at room temperature.
Tightly cap and refrigerate specimens at 2–8°C for up to 48 hours. Freeze samples at ≤ -20°C
if the sample is not assayed within 48 hours. Samples may be stored at ≤ -20°C for up to 13
weeks. Freeze samples only 1 time and mix thoroughly after thawing.
Performed: Within 8 hours of receipt, Available stat.
Reference Range: 3.7-13.9 µmol/L
Method The Atellica IM HCY assay is a competitive immunoassay using direct chemiluminescent.
Description: Technology.
CPT Code: 83090
MERCY TEST NAME HPV DETECTION-HIGH RISK TYPES* MERCY LAB CODE HPVHR
Specimen: Cervical or Vaginal specimen in a ThinPrep solution vial. Indicate source of specimen on
container.
Note: This is NOT a reflex test. It requires a specific request from the provider.
Processing: Send refrigerated to Mayo. Frozen or ambient acceptable. Mayo order code (F17HY).
MERCY TEST NAME IGG INDEX CSF* MERCY LAB CODE CIGG
Specimen: 1 ml spinal fluid plus 2 mL serum from Serum Separator Tube SST or plain red top tube. 2
individual serum samples are required. 2 mL in 2 plastic vials, each containing 1 mL.
Comment: Nursing Service must notify the Lab when the CSF is collected so that the CSF and serum
specimens can be collected within 1 week of each other.
Processing: o Include both CSF and serum specimens, label specimens appropriately.
o Send refrigerated to Mayo. Ambient and Frozen specimens are acceptable. Mayo
order code (SFIG).
Performed: Test set up Monday through Friday.
Reference value: Included with report
Method: SFINC, SFIGS: Nephelometry
ALBSI: Photometric
CPT Code: 82040 Albumin, serum
82042 Albumin, spinal fluid
82784 x2 IgG, serum and spinal fluid
POWERCHART IGG SUBCLASSES
NAME
Comment: This test includes Total IGG with subclasses IGG1, IGG2, IGG3 and IGG4. This test
should not be ordered with IMMG. Order IGM and IGA separately if needed along with IGS.
Specimen: 1 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: Send aliquoted serum refrigerated to Mayo. Ambient, or frozen are acceptable. Mayo
code (IGGS).
Method: Turbidimetry
MERCY TEST NAME IMIPRA DESIPRA* (Norpramin) MERCY LAB CODE IMDS
Method: Turbidimetry
CPT Code: 83521x2
MERCY TEST NAME INSULIN LIKE GF 1* (SOMATOMEDIN-C) MERCY LAB CODE INGF
Specimen: o 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
o Spin down, promptly remove serum from cells.
Processing: Send frozen to Mayo order code (IGFMS).
Performed: Sunday through Friday at 1200 PM
Reference value: Reference ranges included with results.
Method: Liquid Chromatography - Mass Spectrometry (LC/MS)
CPT Code: 84305
Stability: Specimens may be stored for up to 14 days at 2–8°C or stored frozen at -20°C.
Comment: This test should not be performed on patients who have received a vitamin B12 injection or
radiolabeled vitamin B12 injection within the previous 2 weeks.
Performed: 1-3 days. Monday through Friday. Mayo order code (IFBA).
Reference Value: Included in report
MERCY TEST NAME IRON (FE) IBC MERCY LAB CODE IIBC
Comment: Use the following formula to obtain serum UIBC from serum TIBC and iron: TIBC - Iron =
UIBC (μg/dL or μmol/L).
Performed: Within 8 hours of receipt. Available Stat.
Includes: IAB2 (Mayo 89588/IA2) and GAD (Mayo order code GD65S).
Specimen:
3.0 ml serum from no additive serum tube or a Serum Separator Tube (SST).
Processing: Send 1.5 ml serum refrigerated
Specimen:
4 ml EDTA whole blood from purple top EDTA tube.
Processing: o Included a completed Hematopathology Patient Information sheet
o Send ambient to Mayo. Mayo order code (JAK2B).
Performed: 2-5 days. Test set up Monday through Friday 12 p.m.
Reference value: Included with report
Method: Point Mutation Detection in DNA Using Quantitative Polymerase Chain Reaction
Specimen:
0.5 mL serum from a Serum Separator Tube (SST).
Processing: Send refrigerated to Mayo. Mayo order code (JO1)
Performed: 1-2 days. Monday through Saturday; 4 p.m.
Reference value: Included with report
Method: Multiplex Flow Immunoassay
CPT Code: 86235
Comment: For Mercy patients: Order on Powerchart. Indicate specimen source in comment field.
Specimen: Submit entire dried calculi specimen.
Collection Instructions:
1. Have patient collect specimen using the Patient Collection Instructions for Kidney Stones.
2. Prepare stone by cleaning any blood or foreign material from the stone with deionized water.
3. Place stone on a clean filter or paper towel and let dry at ambient temperature for a minimum
of 24 hours. A dry stone will not stick to the walls of the container.
4. Do not place stone directly in a bag. If specimen is received in a bag, either transfer stone
into a screw-capped, plastic container or place bag containing stone in a screw-capped, plastic
container.
5. Indicate source of specimen on the outside of the container (e.g., left kidney, bladder, right
ureter).
6. Repeat steps for each stone received.
-Do not send stone in formalin, surgical gel, or any other liquid as it interferes with the analytic
procedure.
-Do not tape specimen to anything. Tape interferes with the analytical procedure.
-Do not send filter.
If multiple stones are collected and individual testing is desired for each stone, place each stone
into its own container. Testing must be ordered separately on each stone. Each order will be
charged separately.
Processing: Send Ambient (Preferred) to Mayo. Refrigerated or frozen is also acceptable. Mayo order code
(KIDST).
Performed: 4-6 days. Monday through Saturday.
Reference value: Reference ranges included with report.
Method: Infrared Spectrum Analysis
CPT Code: 82365
POWERCHART KOH PREP OTHER
NAME
Order: This test looks for yeast and hyphal elements (fungus) in the sample submitted.
Order the specific KOH test code if the sample is scrapings, hair, skin, nails, tissue.
Order GRAM STAIN DIRECT if the specimen is from the genital tract.
Specimen: Scrapings, hair, nails, and tissue: Submit in a sterile plastic container with a tight-fitting lid.
Performed: Daily.
Specimen: o 1 ml serum from a no additive serum tube or from a Serum Separator Tube (SST).
o Draw specimen immediately before next scheduled dose or at least a minimum of
12 hours after last dose.
Processing: Send refrigerated to Mayo. Ambient or frozen also acceptable. Mayo order code (LACO).
Performed: 1-4 days. Test set up Monday through Saturday.
Reference value: Reference ranges included with report.
Method: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT Code: 80235
Specimen: 0.5 ml body fluid. Place tube immediately on ice and deliver to the Lab within 15 minutes of
collection.
Stability: For body fluid, centrifuge before analysis. Separated CSF samples, may be stored for up to 24
hours at 2–8°C or stored frozen for up to 1 month at -20°C.
Specimen: 5 ml body fluid. Place tube immediately on ice and deliver to the Lab within 15 minutes of
collection.
Stability; For CSF, centrifuge before analysis. Separated CSF samples, may be stored for up to 24 hours
at 2–8°C or stored frozen for up to 1 month at -20°C.
Method
The Atellica CH Lac_2 assay measures lactate in plasma by an enzymatic assay.
Description:
CPT Code: 83605
POWERCHART LACTATE LEVEL
NAME
Specimen: 0.5 ml Sodium Fluoride plasma from gray top tube. Place tube in ice bath immediately after
collection.
Stability: specimens may be stored for up to 1 day at 2–8°C or stored frozen for up to 30 days at -
20°C.
Method
The Atellica CH Lac_2 assay measures lactate in plasma by an enzymatic assay.
Description:
Specimen: Mercy Medical Center - North Iowa Microbiology department performs a stool
LACTOFERRIN, to determine the presence of fecal white cells in a stool sample. A fecal smear
is no longer performed.
Specimen: 1 mL serum from no additive serum tube (Preferred). Serum from a Serum Separator Tube
(SST) is also acceptable.
Draw specimen immediately before next scheduled dose. For sustained-release formulations
only, draw blood a minimum of 12 hours after last dose. Centrifuge within 2 hours of
collection. For red-top tubes, immediately aliquot serum into a plastic vial. For serum gel
tubes, aliquot serum into a plastic vial within 24 hours of collection.
Processing: Send refrigerated (Preferred) to Mayo. Ambient or Frozen is also acceptable. Mayo order
code (LAMO).
Method The Atellica CH RF Reagent 2 is a suspension of uniform polystyrene latex particles coated
Description: with human IgG.
CPT Code: 86431
Method: Calculation
MERCY TEST NAME LEAD WHOLE BLD* MERCY LAB CODE PB1
Specimen: o 500 mcl whole blood from purple top (EDTA tube). Minimum: 200 mcl is acceptable
for capillary collection specimens.
o Alternatively, use blue top (sodium citrate) or green top (sodium heparin) tubes.
o Venous samples (3.0 ml) are required for follow-up of elevated lead levels.
Stability: EDTA specimens are stable 14 days refrigerated.
Cause for
Clotted specimens.
rejection:
Processing: Complete Blood Lead form from University Hygienic Lab (UHL).
Apply bar code label from UHL to the above form. Attach corresponding tube label from UHL
to specimen. Send by U.S. Mail to address below.
Regional Lab Clients: Please order the collection kit directly from University Hygienic Lab.
Regional lab clients are responsible for collection process, mailing kit, billing, and reporting.
University Hygienic Laboratory
Iowa Laboratories Facility
PO Box 249
Ankeny, IA 50021-9959
515-725-1600
Performed: 2 days
Reference value: < 16 years: 0 - 10 mcg/dl
16 and older: 0 - 20 mcg/dl
This test no longer includes a Legionella smear. The Legionella PCR test has replaced the
smear and will need to be ordered separately, if needed. (See Legionella PCR)
Specimen: Bronchial washings, broncho-alveolar lavage, bronchus fluid, chest fluid, chest tube
drainage, empyema, endotracheal specimens, fresh lung tissue, induced sputum, lingual
(lung), lung biopsy, pericardial fluid or tissue, heart valves, pleura, pleural fluid, protected
catheter brush, sputum, thoracentesis fluid, tracheal secretion, transbronchial biopsy, or
trans-tracheal aspirate.
Send in a screw-capped, sterile container.
Refrigerate. Maintain sterility and forward promptly.
RL Client o Write LEGIONELLA CULTURE on order form. Indicate source on the form.
Comments: o Send refrigerated.
Processing: Send specimen in a screw-capped, sterile container. Maintain sterility. Send refrigerated to
Mayo.
Mayo order code (LEGI)
Specimen: 1 mL Bronchial washings, bronchoalveolar lavage, lung tissue, pleural fluid, sputum,
transtracheal aspirate, or tracheal secretions.
Send in a screw-capped, sterile container.
Send Refrigerated. Maintain sterility and forward promptly.
Specimen source is required Mayo order code (LEGRP)
MERCY TEST NAME LEUK LYMPH PHNO TYP* MERCY LAB CODE LKLYPH
Specimen: Blood, Bone marrow, tissue (lymph nodes) other than blood or bone marrow, fluids from
serous effusions.
Peripheral blood: 6 ml peripheral blood in ACD (preferred) or EDTA and sodium heparin are
acceptable. Send whole blood. Include 5-10 unstained peripheral blood smears if possible.
Bone marrow: 1-5 ml bone marrow in EDTA or sodium heparin. Bone marrow specimen is
stable 4 days. On request, we may hold specimen pending pathologists report and request
that test be sent out.
Specimen: o 1.0 ml serum from a no additive serum tube or a Serum Separator Tube (SST).
o Draw blood immediately before next scheduled dose.
o For sustained-release formulations ONLY, draw blood a minimum of 12 hours after
last dose.
o Centrifuge and aliquot serum into plastic vial within 2 hours of collection.
Processing: Send refrigerated to Mayo. Ambient or frozen acceptable. Mayo order code (LEVE).
Stability: Tightly cap and refrigerate specimens at 2–8°C if the assay is not completed within
8 hours. Freeze samples at ≤ -20°C if the sample is not assayed within 48 hours. Freeze
samples only 1 time and mix thoroughly after thawing. The handling and storage
information provided here is based on data.
Comment: Do not use samples that have been stored at room temperature for longer than 8 hours.
Performed: Within 8 hours of receipt. Available stat.
Method
The Atellica IM LH assay is a 2-site sandwich immunoassay using direct chemiluminescent
Description:
technology, which uses constant amounts of 2 antibodies that have specificity for the beta
subunit of the intact LH molecule.
CPT Code:
83002
Specimen: 0.5 ml serum from a no additive serum tube. A Serum Separator Tube (SST) is NOT acceptable.
POWERCHART LIPASE
NAME
Method The Atellica CH Lipase (Lip) assay measures the activity of the enzyme lipase in serum and
Description: plasma by the lipase enzymatic reaction producing methylresorufin, which is determined
spectrophotometrically.
CPT Code: 83690
Patient must be fasting 9-12 hours with no alcohol 24 hours prior to specimen collection.
Reference Range: Cholesterol: Low risk Level <200 mg/dL, moderate risk level 200-239 mg/dL, and high-risk
level ≥ 240 mg/dL.
LDL Cholesterol: Optimal <100 mg/dL, near optimal 100-129 mg/dL, Borderline high 130-159
mg/dL, high 160-189 mg/dL, and very high ≥ 190 mg/dL.
HDL Cholesterol: Low <40 mg/dL and high ≥ 60 mg/dL.
Triglycerides: Normal <150, borderline high 150-199, high 200-499, and very high ≥ 500.
The National Cholesterol Education Program recommends that individuals be seated for at
least 5 minutes prior to phlebotomy to avoid hemo-concentration.
Specimen: 1 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Draw specimen 8-12 hours after last dose (trough specimen). Serum gel tubes should be
centrifuged within 2 hours of collection. Red-top tubes should be centrifuged and aliquoted
within 2 hours of collection.
Processing: Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.
Separated specimens in gel tubes are stable at room temperature 24 hours. Refrigerated up
to 7 days.
Method: Colorimetric
MERCY TEST NAME LIV/KID MICROS T1* MERCY LAB CODE LKM1
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) and no additive serum tube.
Comment: Useful for evaluation of patients with chronic hepatitis (autoimmune).
Processing: Send refrigerated to Mayo. Refrigerated <= 7 days, or frozen acceptable. Mayo order code
(LKM).
Performed: 1-4 days. Test set up Monday, Wednesday, Friday at Mayo.
Reference value: Included with test results.
MERCY TEST NAME LUPUS ANTI PROF* MERCY LAB CODE LUPUS
Specimen: 5.0 mL platelet poor plasma from light-blue top (citrate) tube.
Shipping
1-7 days, Send specimen frozen. Mayo order code (ALUPP).
instructions:
Method: PTC, PTMX, APTTB, DRVT, TT, RPTL, DRVTM, DRVTC, APTTM, STLA: Clot-Based Assay
DIRM: Automated Latex Immunoassay (LIA)
PNP: Activated Partial Thromboplastin Time (APTT) Mixing Test
F_2, FACTV, F_7, F_10, IBETH, F8IS: Prothrombin Clot-Based Assay
F8A, F_9, F_11, F_12: Activated Partial Thromboplastin Clot-Based Assay
FIBC: Clauss Methodology
SFM: Immunoturbidimetric
MERCY TEST NAME LYME DIS SERO EVAL MERCY LAB CODE LYME
Specimen: 1.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Specimen is stable 14 days refrigerated. (A frozen specimen is also acceptable, but not
required)
Comment: This test detects Lyme Disease antibodies IgG and IgM. Each antibody will be reported
separately, along with which proteins the antibodies are detected against.
Note due to reagent supply issues, testing is NOT being performed in house and will be sent
to Mayo.
RL Client
Send 1.0 ml of serum refrigerated to Mercy lab. (Frozen is acceptable, but not necessary)
Comments:
Performed: Available 2-3 days from collection
Method: Immunoblot Microarray
Reference Range: Negative IgG and IgM
CPT Code: 86617 x 2
MERCY TEST NAME Misc. General Lab Designate: MAGNESIUM 24 HOUR MERCY LAB CODE CMIS
URINE in comment
Comment: Collect urine samples in a metal-free container. Urine samples should be acidified to pH 1
Method
Description: The Atellica CH Magnesium (Mg) assay is based on the modified xylidyl blue reaction.
CPT Code:
83735
MERCY TEST Misc. General Lab Designate: MAGNESIUM RANDOM URINE in MERCY LAB CMIS
NAME comment CODE
Special Patients with high concentrations of Gadolinium, Iodine and Barium are known to interfere
Precautions: with most metal tests. If either Gadolinium-, Iodine, or Barium-containing contrast media
has been administered, a specimen cannot be collected for 96 hours.
Specimen: o Draw tubes for metal BEFORE any other tubes are drawn.
o 2 ml Plain, royal blue-top Vacutainer plastic trace element blood collection tube.
o Use alcohol, not iodine to cleanse venipuncture site.
Processing: o Allow the specimen to clot for 30 minutes, and then centrifuge to separate serum
from the cellular fraction. Serum must be removed from cellular fraction within 4
hours of draw. Avoid Hemolysis.
o Remove the stopper. Carefully pour specimen into a Mayo metal-free,
polypropylene vial, while avoiding transfer of the cellular components of
blood. DO NOT insert a pipet into the serum to accomplish transfer, and DO
NOT ream the specimen with a wooden stick to assist with serum transfer.
o Send refrigerated to Mayo. Ambient and frozen also acceptable. Mayo order
code MNS. See Mayo’s LTI for special instructions.
Performed: 1-6 days. Test set up Tuesdays
Reference value: Included with Report.
Method: Dynamic Reaction Cell-Inductively Coupled Plasma-Mass Spectrometry (DRC-ICP-MS)
CPT Code: 83785
Patient Patient should not douche, use any medications or creams in the vagina, or have intercourse
preparation: for 24 - 48 hours prior to specimen collection. Specimen collection is not recommended
during a patient’s menstrual cycle.
Specimen: A vaginal smear from the mid lateral vaginal wall is the area of choice, therefore ensuring an
accurate index evaluation. Obtaining the specimen from any other area will not always
reflect an accurate or true maturation index.
Comment: Please include all appropriate information on the cytology requisition form.
Processing: After slide preparation, cytofixative spray must be applied immediately to ensure
preservation.
Slides must be labeled with patient first and last name in pencil.
Specimen: o Full tube of whole blood from navy blue top EDTA trace metal tube. Minimum 0.3
ml.
o Always draw this tube first if multiple tubes are being drawn.
o Use alcohol, not iodine to cleanse venipuncture site.
o If a syringe is needed, use only Mayo EDTA yellow labeled, metal-free syringe.
Processing: o Leave specimen in tube for shipping.
o Send refrigerated to Mayo. Ambient also acceptable. Mayo order code (HG).
Performed: 1-3 days. Test set up Monday through Saturday.
Reference value: Included with report.
MERCY TEST NAME METAL HVY BLD* MERCY LAB CODE MTHV
Comments: o Mayo Medical Laboratories (MML) is requiring the completion of the T491,
Lead/Heavy Metal Reporting form. Due to state requirements and CDC
recommendations, MML is required to report patient demographic information to
each state on all leads and heavy metals testing. Please click on this link, Mayo
Lead/Heavy Metals Form to complete the form, print, and send with the specimen.
o To be used primarily for Hazardous Materials Teams, such as EMT's or firefighters.
Screens only for Arsenic, Cadmium, Lead and Mercury.
Special Patients with high concentrations of Gadolinium and Iodine are known to interfere with
Precautions: most metal tests. If either Gadolinium or Iodine containing contrast media has been
administered, a specimen cannot be collected for 96 hours.
Specimen: o Draw tubes for metal BEFORE any other tubes are drawn.
o At least 2.5 ml needs to be in the tube. 1 royal blue top EDTA (Monoject trace
element blood collection tube) tube.
o Use alcohol, not iodine to cleanse venipuncture site.
Processing: o EDTA metal free tube: Send as is. Do not centrifuge or aliquot.
o Send refrigerated to Mayo. Ambient and frozen also acceptable. Mayo order code
(HMDB).
Performed: 3 days. Test set up Monday through Saturday
Reference value: Included with report.
Method: Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)
CPT Code: 82175 Arsenic
82300 Cadmium
83655 Lead
83825 Mercury
MERCY TEST NAME HEAVY METALS, URINE* MERCY LAB CODE VMET
Note* If arsenic concentration is greater than or equal to 35 mcg/L, then arsenic speciation
will be performed at an additional charge
Patient o Do not eat seafood for 48 hours before starting or during the collection of the 24
Instructions: Hr. urine.
o High concentrations of gadolinium and iodine are known to interfere with most
metals’ tests. If either gadolinium- or iodine-containing contrast media has been
administered, a specimen cannot be collected for 96 hours.
o 24-Hour volume is required on request form for processing.
Specimen: o 24-hour urine specimen.
o Collect in clean, plastic urine container with no metal cap or glued inserts.
o Refrigerate during collection. No preservative
Processing: o Aliquot 10 ml and indicate total 24-hour volume. Send specimen in clean, plastic
aliquot container with no metal cap or glued insert or into a 6.0 mL urine tube. Mix
well before aliquot is taken.
o Refrigerate specimen within 4 hours of completion of 24-hour collection and send
refrigerated to Mayo. Mayo order code (HMU24).
o The addition of preservative or application of temperature controls must occur
within 4 hours of completion of the collection. See Mayo Test Catalog for special
instructions on collections with preservatives
Performed: 1-4 days. Test set up Monday - Saturday
Reference value: Included with report
Method: Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).
CPT Code: 82175 Arsenic
82300 Cadmium
83655 Lead
83825 Mercury
82175 Arsenic Speciation (if indicated)
MERCY TEST NAME METANEPH, FRAC 24UR* MERCY LAB CODE MTPH
Comment: o Tricyclic antidepressants, labetalol and sotalol medications may elevate levels of
metanephrines producing results which cannot be interpreted. If clinically feasible,
it is optimal to discontinue these medications at least 1 week before collection. For
advice assessing the risk of removing patients from these medications and
alternatives, you may consider consultation with a specialist in endocrinology or
hypertension.
o A single 24- hour urine collection may be used for CATECHOLAMINE
FRACTIONATION [CTCH], METANEPHRINES and VMA [VVMA].
o The specimen must be kept refrigerated during collection.
Specimen: o At start of collection, add 25 ml 50% acetic acid preservative. Use 15 ml 50% acetic
acid for children.
o Refrigerate during collection. Click on 24-hour urine preservative chart for
other acceptable temperatures and additives.
Reference: o Adjust pH to 2.0-4.0 with 50% acetic acid.
o Aliquot 10 ml and indicate total 24-hour volume.
Lab Processing: Separate aliquots must be submitted for Catecholamine Fractionation and VMA if collected
with this specimen. Identify which specimen is for Metanephrine.
Mercy lab Send 10 ml in a 10 ml urine tube refrigerated to Mayo. Ambient and frozen also acceptable.
processing: Mayo order code (METAF).
Performed: 2 days. Test set up Monday through Saturday; 12 p.m. Not reported on Sundays.
Reference value: Included with test results.
Method: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Stable Isotope Dilution
Analysis
CPT Code: 83835
Specimen: Arterial Whole Blood collected in a Heparinized syringe. Minimum volume of 1 ml.
o The tube must be walked to its destination. Do NOT send through the tube
station.
Comment: Respiratory or RN will call lab 87256 to come pick up Arterial specimen that Respiratory is
collecting.
Rejection Criteria: Air in sample, clotted, hemolyzed, unlabeled specimens.
Performed: Within 10 minutes of receiving sample.
Reference Value: 0-4.9%
Method: ABL80 CO-OX Flex
CPT Code: 83050
Specimen: 1.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Frozen and ambient acceptable. Mayo order code (MMAS).
Performed: Monday through Friday; Continuous until noon.
Reference Value: Included in report.
Method: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT Code: 83921
Method: Turbidimetric
CPT Code: 82043
MERCY TEST NAME MISC GENERAL LAB MERCY LAB CODE CMIS
POWERCHART General Lab Miscellaneous (MC) Non-Blood or Miscellaneous Lab Procedure (MC) Non-
NAME Blood
Comment: Indicate reference lab test code with name of test desired.
If ordering in Cerner Powerchart a task will be created for nursing to collect specimen.
MERCY TEST NAME MITOCHOND AB, M2* MERCY LAB CODE MTAB
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube
Comment: Duplicate testing if ordered with ALDP
Processing: Send refrigerated to Mayo. Mayo test code (AMA).
Performed: 1 day. Test set up Monday through Saturday; 11 a.m.
Reference value: Included with test results.
Method: Enzyme Immunoassay (EIA)
CPT Code: 86381
POWERCHART General Lab Miscellaneous Non-Blood
NAME
(MONKEY POX) ORTHOPOX, conclusion, Qualitative Real- Time PCR*
MERCY TEST NAME MISC GENERAL NONBLOD* MERCY LAB CODE CMISN
Requirements: Clinicians must report suspected monkeypox cases to the IDPH Center for Acute
Disease Epidemiology (CADE) as soon as monkeypox is suspected and PRIOR TO
COLLECTING SPECIMENS.
Specimens approved by IDPH for testing at SHL should be submitted using a current IDPH
Epidemiological Investigation Test Request Form (TRF) obtained by contacting IDPH
CADE.
IDPH will consult with CDC and SHL to determine the need and plan for laboratory testing.
Contact Information:
Clinics should not send specimen to MercyOne North Iowa Medical Center. Do not
order enter an order for MercyOne North Iowa. Refer to SHL and CDS website for
ordering and transporting. Contact CDS courier to schedule pick-up of specimens:
http://cdsofiowa.com / or 515-289-9990
The provider must contact the IDPH to be provided access to the IDPH Epidemiological
Specimen: Investigation Test Request Form (TRF).
Each lesion will require an order. The recommendation is to select two lesions to
swab.
The kit will have 2 swabs. Swab one selected lesion vigorously with both swabs from a
kit. Each swab must be placed into its own container and labeled. The swab may be placed
in viral transport media (VTM) or a dry swab tube. Lesion crust is also acceptable. Place
both swabs for the single lesion back into the kit’s bag. Each kit (two swabs of the same
lesion) must be accompanied by the completed IDPH Epidemiological Investigation Test
Request Form (TRF).
2 lesions require 2 orders and 2 kits. Per kit: two swabs and one TRF.
Transport Inpatient or ED should walk to lab properly packaged specimen. Lab will order on SHL
website and schedule for transport.
Refrigerated specimens- ship on ice packs; acceptable 7 days. Frozen specimens- ship on
dry ice; acceptable 30 days
Reject if cotton or rayon swabs; wooden swabs; M4 media, UTM; FlexTrans or Room Temp
Days Performed: Specimen referred to State Hygienic Laboratory at University of Iowa; Set up daily; Report
available: 2-3 days
MERCY TEST NAME MONOCLONAL PRT STY, 24UR* MERCY LAB CODE MCPSU
Cautions: Monoclonal gammopathies are rarely seen in patients younger than 30 years of age.
Hemolysis may cause a discrete band on protein electrophoresis, which will be negative on
M-protein isotyping.
Processing: Aliquot between 30 mL and 50 mL urine into plastic, 60-mL urine bottle.
Methods: PTU: Turbidimetry, PEU: Agarose Gel Electrophoresis, MPTU: Matrix-Assisted Laser
Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
84166
0077U
NOTE: Clients should order both Mayo QMPSS and Mayo FLCS for diagnostice cases. QMPSS
should be ordered on its own for monitoring cases.
Processing: 2 mL total serume in 2 separate plastic vials, each containing 1 mL of serum. Send refrigerated.
Mayo order code (QMPSS)
MERCY TEST NAME MONCL PRT QNT 24UR* MERCY LAB CODE VELC
Comment: 05/16/2023 Mayo Update: Electrophoresis, Protein, 24 Hour, Urine (Mayo: EPU) was made
obsolete and replaced with Monoclonal Protein Quantitation, 24 Hour, Urine (Mayo:
MPQU). This is due to an improved methodology to detect monoclonal proteins.
Cautions: Patients suspected of having a monoclonal gammopathy may have a normal urine protein
electrophoretic pattern, and these patients should have M-protein isotyping performed.
Monoclonal gammopathies are rarely seen in patients younger than 30 years of age.
Hemolysis may cause a discrete band on protein electrophoresis, which will be negative on
M-protein isotyping.
Processing: Aliquot between 30 mL and 50 mL urine into plastic, 60-mL urine bottle.
Methods: PTU: Turbidimetry, PEU: Agarose Gel Electrophoresis, MPTU: Matrix-Assisted Laser
Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
84166
MERCY TEST NAME MONO PRT QNT R UR* MERCY LAB REPU
CODE
Processing: Mercy Lab Processing only: Aliquot into 60-mL urine bottle. Send refrigerated. Mayo order
code (RMPQU).
MERCY TEST NAME MONOSCREEN (Heterophile Titer) MERCY LAB CODE MOSC
Specimen: o 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
o Heparin or EDTA plasma.
o Remove serum in no additive serum tube or plasma from cells.
Stability: 48 hours refrigerated. Freeze if >48 hours.
Comments: Make sure collection site (Nasal) is indicated on Culturette label also.
If the provider is checking for a nasal infection and wants to treat the patient, contact the
Microbiology lab department for correct ordering of this type of request.
Intended Use: Testing is used to monitor Vancomycin de-escalation in the treatment of pneumonia.
Regional Laboratory clients: Use order MRSANX for any of your submissions.
Stability: Send Culturette at room temperature within 24 hours. Stable for 5 days refrigerated.
Comments: Make sure collection site (Nasal) is indicated on Culturette label also.
If the provider is checking for a nasal infection and wants to treat the patient, contact the
Microbiology lab department for correct ordering of this type of request.
Intended Use: The intended use of this surveillance assay is to screen patients for MRSA
colonization/decolonization. There is NO susceptibility testing performed with this assay.
For non-Mercy Locations: Order code will be MRSANX. This testing will be billed back to
the ordering facility.
Stability: Send Culturette at room temperature within 24 hours. Stable for 5 days refrigerated.
Performed: Within 8 hours of receipt.
Method: PCR
Enter site of collection in specimen source area. Make sure collection site is indicated on
Culturette.
If the provider is checking for infection and wants to treat the patient, see Culture wound
other (WND/ABS CLT/GS).
Intended Use: The intended use of this assay is to screen Mercy Hospital patients for MRSA colonization.
CLINICS: The wound surveillance assay is not intended for clinic use. Nasal surveillance assay
is the only appropriate assay, in this instance. Wounds should continue to be ordered as a
culture, to look for MRSA.
HOSPITAL REFERENCE LABS: The wound surveillance assay is not intended for hospital
reference lab use, unless a wound surveillance protocol has been established by the
reference lab's infection prevention department for this type of specimen.
Comment: This test requires both CSF and serum. Please notify Lab when this test is ordered so that
a blood specimen can be collected at the same time.
Includes: Kappa Free Light Chain, CSF, possible additional test result if appropriate for serum bands,
CSF bands, CSF Olig Bands Interpretation
Specimen: 1.0 ml CSF and 1.0 ml serum from no additive serum tube or Serum Separator Tube
(SST). Minimum 0.5 ml CSF and 0.5 ml serum. Nursing Service must notify the Lab when CSF
is collected so that the CSF and serum specimens can be collected. Spinal Fluid must be
obtained within 1 week of serum draw.
Processing: o DO NOT perform any CSF testing at Mercy Laboratory until AFTER CSF
specimen has been processed for Mayo testing.
o 1 ml CSF, send in original tube when possible. Label tube as CSF.
o 1 ml serum in vial labeled as such.
SEND ALL SPECIMENS FROZEN TO MAYO. LABEL 1 ALIQUOT CSF (1.0 ML) AND 1
ALIQOUT SERUM (1.0 ML)
Specimen: 1 ml Serum from a Serum Separator Tube (SST) or no additive serum tube
Processing: Send refrigerated to Mayo (Frozen is acceptable). Mayo order code (MMPGM).
Comment: This surveillance testing is for symptomatic patients only. The State Hygienic Lab Mumps
surveillance is a buccal (mouth) PCR. A SHL Viral and Bacterial PCR Test Request Form will be
required for testing.
Specimen: 1 buccal swab (mouth).
Collection: Collect sample using a SHL Virus Isolation and Detection Kit (contains M4-RT viral transport
medium and swab) or equivalent virus transport media. Kits may be request through
MercyOne North Iowa Lab.
Buccal (Oral) Swab: The buccal cavity is the space between the cheek and teeth. The
parotid duct drains in the space near the upper molars. Massage the parotid gland area just
in front of the ear and near the angle of the jaw for 30 seconds prior to collecting secretions
on the swab. Swab the buccal cavity by sweeping the swab near the upper molar to the
lower molar. Place swab in M4-RT viral transport medium and do not remove swab.
Reason for rejection include Frozen samples, sample obtained on cotton-tipped, wooden-
shafted, or calcium alginate swabs.
Performed: Monday through Friday, at State Hygienic Lab, Iowa City, Ia.
Method: PCR
CPT Code: 87798
Comment: o Notify Pathology Department 24 hours in advance. Test done Monday through
Wednesday only.
o Complete a manual Pathology Specimen form and a Muscle Histochemistry
Information sheet.
Specimen: Excise 2 samples using sterilized muscle clamps. Sterilized biopsy forceps are available from
the Histology Department. Send immediately to the Histology Laboratory for processing.
Processing: Send specimen frozen on dry ice to Mayo. Mayo test code (MPCT).
Preformed: 7 days. Test set up 1-2 times a week at Mayo.
Reference value: Interpretive report provided.
CPT Code: 88314 X 7 acetic non-specific esterase, acid phosphatase, alpha-naphthyl, cytochrome
oxidase, NADH dehydrogenase, phosphorytase, and succinic dehydrogenase stains.
88314 X 3 ATPase acid-alkaline stain
88313 X 4 Hematoxylin-and-eosin, oil red O, periodic-acid Schiff, and trichrome stains.
88305 surgical pathology exam.
Specimen: 1.0 ml of serum from a red top tube. Serum gel/SST is NOT acceptable.
Processing: Send refrigerated. Frozen and ambient acceptable. Mayo order code (MPA).
Performed: 1-3 days. Monday through Sunday; Varies
Reference value: Included in report.
Method: Tandem Mass Spectrometry (MS/MS)
CPT Code: 80180
MERCY TEST NAME MYCPLSMA PNEUN PCR* MERCY LAB CODE MYCPCR
**Update: 10/10/23: Respiratory fluid and body fluid specimens submitted for this test will
no longer be accepted if sent in viral transport media (VTM). These fluids should be
transported in a sterile container not containing media.
RL Client Write Mycoplasma Pneumoniae by PCR, Mayo code - MPRP on the requisition, Specimen
Comments: Source information is required.
Send specimen refrigerated to Mercy Lab
Processing: Send specimen refrigerated to Mayo. Mayo order code (MPRP).
Method: Rapid Polymerase Chain Reaction (PCR) using Light Cycler and Fluorescent Resonance
Energy Transfer (FRET)
MERCY TEST NAME MYCO.PNEUM IGG, IGM* MERCY LAB CODE MYCOGM
Specimen: 0.5 ml of serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send specimen refrigerate. Frozen acceptable. Mayo order code (MYCO).
Performed: 1 day, Monday through Friday; 9 A.M.
Comment: Cautions: The use of hemolyzed, lipemic, bacterially contaminated, or heat-inactivated
specimens should be avoided. The continued presence or absence of antibodies cannot be
used to determine the success or failure of therapy.
Reference value: Included with results.
Method: Enzyme Immunoassay (EIA)
CPT Code: 86738 x 2
86738-Mycoplasm pneumoniae by indirect IFA (if appropriate)
MERCY TEST NAME MDS BONE MARROW* MERCY LAB CODE MYEFL
Specimen: 2-5 ml bone marrow specimen in ACD (preferred), EDTA and Sodium heparin bone marrow
samples are also acceptable.
Processing: Label specimen as Bone Marrow. Include 5 to 10 unstained bone marrow aspirate smears.
Send AMBIENT to Mayo Medical Laboratories for testing.
Mayo Code - (MYEFL).
Performed: Specimens are processed and reported Monday-Saturday. Maximum Laboratory time 4 days
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Comment: Useful for evaluation of patients with vasculitis and renal disease. If Cytoplasmic Neutrophil
ABS is ordered, and p-ANCA is positive, Myeloperoxidase Antibodies, serum will be done and
charged per Mercy Medical Center – North Iowa Lab policy. Test is also included in
Cytoplasmic Neutrophil Antibodies Vasculitis Panel (VAPNL).
Processing: Send refrigerated to Mayo. Frozen acceptable. Mayo order code (MPO).
Performed: 4 days. Test set up Monday through Saturday; 4 p.m..
Reference value: Reference ranges included with results.
Method: Multiplex flow immunoassay.
CPT Code: 83516
Specimen: Peripheral Blood: 3 mL whole blood in EDTA or yellow top ACD. Send specimen in original
tube. Do NOT aliquot. Label specimen as blood.
Bone marrow: 2 mL bone marrow in EDTA or yellow top ACD. Send specimen in original
tube. Do NOT aliquot. Label specimen as bone marrow.
Processing: Send at room temperature. Refrigerated is acceptable. Mayo order code (MPNR).
POWERCHART MYOGLOBIN
NAME
Specimen: 5 ml preservative free random urine in10 mL plastic myoglobin transport tube.
Processing: o If sample is ambient, aliquot the urine to a urine myoglobin transport tube within
one hour of collection (Supply T691). Refrigerate specimen.
o If sample is refrigerated, aliquot the urine to a urine myoglobin transport tube
within two hours of collection.
Caution: An elevated level of myoglobin in urine does not identify the clinical disorder. Urine collected
with acid as preservative will NOT be valid because acid interferes with analyte integrity.
Performed: 1-2 days. Test set up Monday through Sunday, continuously.
Reference value: Included with test results
Method: Latex Particle-Enhanced Immunoturbidimetric Assay.
CPT Code: 83874
MERCY TEST NAME NEONT MET SCR* MERCY LAB CODE NNT
Comment: If the State lab requests the patient to be retested due to the results from the initial testing,
Laboratory staff can order the NEONT MET SCR RPT*, Sunquest order code
NNTR. Neonate Met Scr Rprt* is not orderable from Powerchart. Lab must order. Connected
to the Sunquest order code NNTR is a processing fee only. The NNTR is not to be used when
a second collection is required due to an error in the collection process.
Recollection due to a lab error should be the Sunquest order code NNT with the first test
being credited. Nursery is to notify ER as to which baby will be returning. A repeat INMSP
form will be provided to the Lab by the State Lab. A repeat specimen must be collected
within 14 days of age if the first specimen was collected prior to 24 hours after birth.
MERCY TEST NAME NEONT MET SCR RPT* MERCY LAB CODE NNTR
MERCY TEST NAME NICOT METABOLIT UR* MERCY LAB CODE NICOU
Processing: Send refrigerated to Mayo. Ambient and frozen also acceptable. Mayo order code (NICOU).
Performed: 2-5 days, Monday through Sunday.
Reference value: Included with results.
Method: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT Code: G0480 / 80323
MERCY TEST NAME OCCULT BLOOD FECAL ICT MERCY LAB CODE OBFS
SCREEN
Specimen: o 0.5 ml CSF and 0.5 ml serum from plain red top tube or serum gel tube.
o Minimum 0.4 ml CSF and 0.4 ml serum.
o Nursing Service must notify the Lab when CSF is collected so that the serum
specimen can be collected.
o Spinal Fluid must be obtained within 1 week of serum draw.
Processing: o DO NOT perform any CSF testing at Mercy Laboratory until AFTER CSF
specimen has been processed for Mayo testing.
o 0.5 ml CSF, send in original tube when possible. Label tube as CSF.
o 0.5 ml serum in vial labeled as such.
o Record on Mayo batch list: # of ml of CSF sent.
o SEND ALL TESTS REFRIGERATED TO MAYO. LABEL 1 ALIQUOT CSF (0.5 ml) AND 1
ALIQUOT SERUM (0.5 ml). Mayo - (OLIG). AMBIENT AND FROZEN ACCEPTABLE.
Performed: Monday through Saturday
MERCY TEST NAME OPIATES UR* CONFIRMATION MERCY LAB CODE UOPIAT
Performed: Monday-Friday
Reference
Included in report.
Value:
Method: Liquid Chromatography - Tandem Mass Spectrometry (LC - MS/MS)
CPT Code: G0480/ 80361 / 80365
Patient
Avoid taking large doses (greater than 2.0 g orally/ 24 hours) of Vitamin C during collection.
preparation:
Specimen: o 24-hour urine collection.
o Add 5 mL of diazolidinyl urea (Germall) as a preservative at start of collection OR
refrigerate specimen during and after collection.
o Collect in metal free container with no metal cap or glued insert.
o Refrigerate during collection.
o Specimen pH should be between 4.5 and 8 and will stay in this range if kept
refrigerated during and after collection. Specimens with pH > 8 indicate bacterial
contamination, and testing will be cancelled.
o DO NOT attempt to adjust pH as it will adversely affect results.
Cause for
Samples collected in or sent in containers with metal caps will not be tested.
rejection:
Processing: o Transfer 4 ml urine to 5 mL metal-free container. Mix well before the aliquot is
taken.
o Indicate total 24-hour volume.
o Send refrigerated to Mayo. Mayo order code (OXU). Frozen and Ambient are
acceptable.
o Click on 24-hour urine preservative chart for other acceptable temperatures and
additives.
o Diazolidinyl Urea (Germall) is listed as preferred, but Mercy Lab does not
have this in our inventory.
Performed: Results 3-5 days. Monday through Saturday.
Method: Enzymatic using Oxalate Oxidase.
CPT Code: 83945
Specimen: 1 mL serum from a no additive serum tube. a Serum Separator Tube (SST) are NOT
acceptable.
Specimen: Arterial, mixed venous, venous collected in a Heparinized syringe. Minimum volume of 1 ml.
non-Heparinized syringes are also acceptable.
o The tube must be walked to its destination. Do NOT send through the tube
station.
Comment: RN will page lab on pager #420 to pick up specimen after collect.
Rejection Criteria: Air in sample, clotted, hemolyzed, unlabeled specimens, or received greater than 10 minutes
after collection.
MERCY TEST NAME PANCREATIC ELASTASE STOOL* MERCY LAB CODE PANCS
Specimen: Collect 5 gm random stool submitted in a container with a tight-fitting lid. No preservative
Processing: Specimen may be stored refrigerated up to 72 hours following collection. Send frozen to
Mayo. Mayo order code (ELASF).
Separate specimens must be submitted when multiple tests are ordered. If only a
single specimen is collected, it must be split prior to transport.
Processing: Send refrigerated (Preferred) to Mayo. Ambient or frozen is also acceptable. Mayo order
code (PAVAL).
Performed: 10-17 days. Profile Tests: Monday through Sunday; Reflex tests: Varies.
CPT Code: 86255 x 9, 83519, 86596 If Indicated: 84182-AGNBS, 84182-AMIBS, 84182-AN1BS, 84182-
AN2BS, 86255-CS2CS, 84182-CRMWS, 86255-LG1CS, 84182-PC1BS, 84182-PCTBS, 86256-
AGNTS, 86256-APHTS, 86256-AN1TS, 86256-AN3TS, 86256-CRMTS, 86256-PC1TS, 86256-
PC2TS, and 86256-PCTTS.
POWERCHART PARASITE EXAM
NAME
MERCY TEST NAME PARASITE EXAM* (Ova & Parasites or O & P) MERCY LAB CODE PARSIT
Note: This test should be ordered when suspicion of parasitic infection is based on travel history in
endemic areas or when a patient is immunocompromised.
Comments: It is strongly recommended that multiple stool specimens be submitted for ova and parasite
analysis. At least 3 specimens should be collected, 1 each day or on alternate days (over a
maximum 10-day period).
Parasites are shed irregularly in stool and examination of a single specimen does not
guarantee detection.
Test will NOT detect Cryptosporidium. See “Cryptosporidium” if this test is desired.
This test is useful for patients who have traveled to foreign countries, or an area of the USA
where helminth (worm) infections have been reported with some frequency.
For patients who have not traveled, order Giardia and Cryptosporidium Antigen testing
(GLCP) instead of Parasitic Exam, performed at MercyOne Lab.
Patient Specimen collection should be delayed for 7 to 10 days after administration of barium,
Preparation: bismuth, kaolin, magnesia, castor oil or mineral oil, and 2 to 3 weeks after antibiotics have
been given since these may interfere with identification of protozoa.
Specimen: Stool delivered within 30 minutes of collection: 5-10 gm of feces submitted in a clean
container with tight fitting lid. Mercy lab will transfer the stool into the Ecofix transporter
(within 30 minutes of collection).
If stool will not be delivered within 30 minutes of collection: The patient will need to transfer
the stool into an Ecofix transporter before the delivery of specimen to the lab. Transfer
enough stool to bring the liquid level up to the fill line, indicated on the Ecofix preservative
vial. Mix the contents of the tube with the spoon, twist the cap tightly closed and shake
vigorously until the contents are well mixed.
Mercy Inpatient Specimens collected from inpatients after the fourth hospital day will NOT be tested
Comments: without prior approval from the Microbiology Department.
Processing: Send ambient (preferred) to Mayo. Refrigerated is also acceptable. Mayo order code (OPE).
Method: Microscopic
The Intraoperative Mode testing is NOT recommended for use in routine PTH testing
Reference value: 12-88 pg/ml
Method: Immunoenzymatic("sandwich") assay.
CPT Code: 83970 Parathyroid Hormone
POWERCHART PARATHYROID HORMONE INTACT
NAME
Serum should be physically separated from cells as soon as possible with a maximum limit of
2 hours from the time of collection.
Performed: Within 8 hours of receipt. Available stat. May be used for Intraoperative testing as well.
Reference Range: 12-88 pg/ml
Method The Atellica IM PTH assay is a 2-site sandwich immunoassay using direct chemiluminescent
Description: technology, which uses constant amounts of 2 anti-human PTH antibodies.
CPT Code: 83970 Parathyroid Hormone
Specimen: o 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
o Maintain sterility of specimen.
o Send to Mayo refrigerated. Frozen and ambient also acceptable.
o Mayo order code (PARVs).
Cause for
Hemolyzed specimens not acceptable.
rejection:
Performed: 1 day. Test set up Monday through Friday; 11 a.m.
Reference Values: Included in report
POWERCHART
PERTUSSIS PCR
NAME
Specimen: Nurse to collect. Use the kit provided by Mercy Microbiology Lab.
o Collect one nasopharyngeal swab (provided in kit) by passing the swab through the
nares of the patient until resistance signifies the swab has reached the posterior
wall of the pharynx. Rotate axially and hold for 30-60 seconds or until coughing
occurs or the patient resists. Perform this same technique for both nares, using the
one swab provided.
o Place the swab in the empty tube provided and cut the swab off, ensuring it is cut
short enough to allow the lid to be screwed on the tube. Screw the lid on the tube
securely. Write the patient's name, date, and time of collection on the tube that
contains the swab.
o Complete the patient information form and return with the specimens to Mercy
Lab. Submit specimen at room temperature.
Alias:
o BORDETELLA PERTUSSIS PCR
RL Client Collection kits can be requested directly from the State Hygienic Lab if RL clients
o
Comments: would like to send the kits directly from their site, otherwise kits can be requested
from Mercy Microbiology.
Processing: Specimens are sent to State Hygienic Lab, Iowa City (SHL)
Method: PCR
MERCY TEST NAME PHENCYCLIDINE UR* PCP CONFIRMATION MERCY LAB CODE UPCP
Specimen: 0.5 mL
Stability: Specimens may be stored for up to 8 hours at 25°C or for up to 2 days at 2–8°C or stored
frozen for up to 30 days at -20°C.
Comment: Indicate time of last does in comment field.
Performed: Completed at Mayo laboratories with 1 day’s turnaround time: Mayo code - (PNTFT)
Comment: Test available ONLY Monday-Friday 0800-1530 by appointment only. Appointments can be
set up by calling the cancer center scheduling desk at 641 428 6321. Not available stat
except with special arrangements between laboratory and staff physician. A written order by
the physician is necessary. Lab will order a hemoglobin on any patient, not followed with
ferritin values, who has not had a hemoglobin performed at MMC-NI within the past 30 days
if the patient present without any pre-phlebotomy orders. If the following criteria are not
met, pathologist authorization must be given to proceed with the phlebotomy.
Specimen: Collect the specimen on a pinworm paddle. Paddles are available from the Microbiology
Department.
MERCY TEST NAME PLASMA CELL DNA MERCY LAB CODE PCPRO
Performed: 2 days. Specimens are processed Monday-Sunday. They are reported Monday-Friday.
Reference value: Included with test results
Method: Flow Cytometry, DNS Content, Cell Cycle analysis
CPT Code: 88182 Flow Cytometry Cell cycle or DNA Analysis
88184 Flow Cytometry First Marker
88185 (x5) Flow Cytometry, Each Additional Marker
88187 Flow interpretation: 2 to 8 markers
Specimen: Draw one waste tube (blue top tube or plain red top tube.) followed by 2 blue top Greiner
tubes. Blue top Greiner tubes fill only half full. Use 21 gauge or larger needle to draw. If
drawing with a syringe, use first syringe to draw waste or other tests, change syringe and
use 2nd syringe for this test. GENTLY invert tube 5 times to mix. DO NOT shake or send in
pneumatic tube system. DO NOT refrigerate or centrifuge. Always draw blue top tubes
before purple top tubes. Flag top of tubes for indication not to spin.
Cause for
Specimen older than 4 hours, refrigerated, or centrifuged.
rejection:
Performed: Within 2 hours of receipt. Available stat.
Reference range: Results are reported in ARU (Aspirin Reaction Units).
>550 ARU - Platelet dysfunction consistent with aspirin has not been detected.
Verify Now Aspirin Assay is a qualitative assay to aid in the detection of platelet dysfunction
due to aspirin ingestion. Other uses of the ARU value are not endorsed by Accumetrics and
is not FDA cleared.
Interference Studies:
The following medications may cause a change in platelet function.
Patients who have been treated with eptifibatide (Integrillin) and tirofiban (Aggrastat) should
not be tested for 48 hours, or abciximab (ReoPro) for 14 days.
Anti-Platelet agents can inhibit platelet function and may result in a decreased ARU value
independent of the effects of aspirin. Average duration times are Plavix and Ticlid - 5 days,
Aggrenox - 10 days, Persantine and Platell/Cilostazol - 12 hours.
NSAID's inhibit platelet function, but not irreversibly like aspirin. Average times for inhibitory
effects for each drug are Ibuprofen - 8 hours: Naproxen, Diclofenac, Indocin - 24 hours,
Feldene 50 hours.
MERCY TEST NAME PLT INHIB P2Y12 MERCY LAB CODE PLTIHB
Cause for
Specimen older than 4 hours, refrigerated, or centrifuged.
rejection:
Performed: Within 2 hours of receipt. Available stat.
Therapeutic range: Therapeutic range is
Normal range: PLT Inhibition P2Y12: 194-418 PRU (P2Y12 Reaction Units) for person not
taking thienopyridines drugs.
Interfering Substances:
MERCY TEST NAME PLATLTS FOR INFUS MERCY LAB CODE TPLT
Comment: A Platelet Count must also be ordered if one has not been performed at Mercy Medical
Center-North Iowa within one week prior to platelet infusion. Pheresis platelets and
Acrodose platelets are stocked depending upon our blood supplier's availability. Both are
prestorage leukoreduced and equivalent in dosage to 6-8 random platelets.
An order for pheresis platelets may be filled with either product. If irradiation is need,
indicate so in the comment field for EACH order placed. It is not sufficient to send a message
to cover all orders. Call the Lab when irradiated platelets are ordered. Orders for irradiated
platelets must be entered into the computer and called to the Lab no later than 1515,
Monday through Friday. Special arrangements must be made if irradiated products are
requested after 1515 or on weekends or holidays.
PHERESIS PLATELETS:
Order PLATELETS FOR INFUSION (TPLT)
Units ordered: The default is 1 unit. Any additional instructions, such as IRRAD (irradiation
needed), can be entered also at this time. All pheresis platelets are leuko depleted and
therefore a leukocyte (WBC) removal filter is not needed.
Specimen: No specimen is needed provided the patient's blood type is on file in the lab.
Processing: If RH negative units are required, they may have to be specially ordered from TBCCI.
Performed: Available stat.
CPT Code: P9019
POWERCHART PNEUMONIA PANEL PCR
NAME
MERCY TEST NAME PNEUMONIA PANEL PCR MERCY LAB CODE BFPNEU
MERCY TEST NAME PORPHY QNT 24UR* MERCY LAB CODE PRPQ
Patient Patient should abstain from alcohol for 24 hours prior to, as well as during, collection.
preparation: Include a list of medications the patient is currently taking.
MERCY TEST NAME PORPHY QNT RNDM UR* MERCY LAB CODE PORPHR
Method: The methods for measurement of electrolytes include flame photometry, spectrophotometry
and direct or indirect ion selective electrode potentiometry.
The A‑LYTE Na, K, and Cl assays use indirect Integrated Multisensor Technology (IMT). There
are four electrodes used to measure electrolytes. Three of these electrodes are ion-selective
for sodium, potassium, and chloride. A reference electrode is also incorporated in the
multisensor.
CPT Code: 84132
Method The methods for measurement of electrolytes include flame photometry, spectrophotometry
Description: and direct or indirect ion selective electrode potentiometry.
The A‑LYTE Na, K, and Cl assays use indirect Integrated Multisensor Technology (IMT). There
are four electrodes used to measure electrolytes. Three of these electrodes are ion-selective
for sodium, potassium, and chloride. A reference electrode is also incorporated in the
multisensor.
CPT Code: 84133
POWERCHART PREALBUMIN
NAME
MERCY TEST NAME PREG TEST SERUM MERCY LAB CODE HCGS
MERCY TEST NAME PREG TEST UR QAL MERCY LAB CODE HCGU
MERCY TEST NAME PRENATAL PROFILE (WITH HIV) MERCY LAB CODE PTYS & PNP
Comment: Blood Bank Antibody titer is not included. If desired, it must be ordered separately by the
physician.
All reflex testing will be completed at an additional charge. This includes the following:
-Antibody screen is positive; the antibody ID will be done.
-HIV test is positive; a HIV evaluation will be done.
-Hep B Surface Ag is positive, neutralization testing will be done.
-Syphilis test is positive, RPR testing will be done.
Specimen: Two 8.5 mL Serum Separator Tubes (SST), One 6 mL pink top (EDTA) tube, and one 3 mL
purple top (EDTA) tube.
Specimen Minimums:
Pink tube: 2 mL for ABO/RH & Antibody Screen.
Purple tube: 1 mL for CBC. May also use a capillary tube minimum of 300 mcl.
SST tube: 3-4 mL serum.
MERCY TEST NAME PRENATAL PROF (NO HIV) MERCY LAB CODE PNPO & PTYS
Comment: Blood Bank Antibody titer is not included. If desired, it must be ordered separately by the
physician.
All reflex testing will be completed at an additional charge. This includes the following:
-Antibody screen is positive; the antibody ID will be done.
-Hep B Surface Ag is positive, neutralization testing will be done.
-Syphilis test is positive, RPR testing will be done.
Reference Lab Clients: Please specify on order form PNP/NO HIV. If nothing is specified, a
Prenatal Profile with HIV will be done.
Specimen: Two 8.5 mL Serum Separator Tubes (SST), One 6 mL pink top (EDTA) tube, and one 3 mL
purple top (EDTA) tube.
Specimen Minimums:
Pink tube: 2 mL for ABO/RH & Antibody Screen.
Purple tube: 1 mL for CBC. May also use a capillary tube minimum of 300 mcl.
SST tube: 3-4 mL serum.
Comments: DO NOT order an additional Phenobarbital. Indicate time last dose in the comment field.
Specimen: 0.5 ml serum from a Serum Separator Tube (SST). Send specimen Refrigerated. Mayo order
code (PRMB).
Performed: 1 day. Monday through Sunday.
Reference value: Included with test results.
Method: Immunoassay
CPT Code: 80188 Primidone
80184 Phenobarbital
Tightly cap and refrigerate specimens at 2–8°C if the assay is not completed within 8 hours.
Freeze samples at ≤ -20°C if the sample is not assayed within 48 hours. Freeze samples up to
5 times and mix thoroughly after thawing.
Method The Atellica IM PRGE assay is a competitive immunoassay using direct chemiluminescent
Description: Technology.
CPT Code: 84144
MERCY TEST NAME PROSTATIC ACID PHOS* MERCY LAB CODE ACPH
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo order code (PR3).
Performed: 1 day. Monday through Saturday.
Reference Value: Reference ranges included with results.
o Centrifuge specimen.
o Aliquot plasma (leaving some above the cells) to a plastic centrifuge tube.
o Centrifuge the aliquot tube.
o Pipette plasma (leaving some above the bottom of the tube) to another plastic
aliquot tube.
o Send frozen to Mayo. Mayo order code (CFX).
Performed: 1 - 3 days. Monday through Friday.
Reference value: Included with test results.
Method: Amylolysis of Chromogenic Substrate
CPT Code: 85303
Note: Due to manufacturer supply backorder at Mayo, PCAG is temporarily unavailable. Recommended
alternative test is Mayo: FPCTA. Order CMIS: FPCTA (Protein C, Total Antigen). See Mayo Lab Test Index for
specimen requirements.
Stability: Urine specimens may be stored for up to 4 days at 2–8°C or stored frozen at or below -20°C.
o Centrifuge specimen.
o Aliquot plasma (leaving some above the cells) to a plastic centrifuge tube.
o Centrifuge the aliquot tube.
o Pipette plasma (leaving some above the bottom of the tube) to another plastic
aliquot tube.
o Send frozen to Mayo. Mayo test code (SFX).
Performed: 1 - 4 days. Monday through Friday.
Reference value: Included with test results.
Method: Optical Clot-Base
CPT Code: 85306
Specimen: 1.0 mL platelet-poor plasma from blue top (citrate) tube. Must send 0.5 mL in 2 separate
aliquot tubes.
Processing Double spin specimen to ensure that all platelets are removed:
o Centrifuge specimen
o Aliquot plasma (leaving some above the cells) to a plastic centrifuge tube.
o Centrifuge the aliquot tube.
o Pipette plasma (leaving some above the bottom of the tube) to another plastic
aliquot tube.
o Send frozen to Mayo. Mayo order code - (PSTF)
Comment If this initial Protein S Antigen free testing is abnormal, the Protein S Antigen Total will be
performed at an additional charge.
Performed: 1 - 3 days. Monday through Friday
Reference value: Included in report
Method: Automated Latex Immunoassay (LIA)
CPT Code: 85306 Free Protein S Antigen
85305 Protein S Antigen Total (If Appropriate)
POWERCHART PROTEIN
NAME
MERCY TEST NAME PROT TTL CSF MERCY LAB CODE CPRT
Comment: Centrifuge every CSF specimen and analyze the supernatant. Specimens should not contain
blood and should avoid hemolysis.
Performed: Within 8 hours of receipt. Available stat
Reference Range: 0-3 months: 20-100 mg/dl
>3 months: 15-45 mg/dl
Method
The Atellica CH UCFP assay is an adaptation of pyrogallol red-molybdate method.
Description:
CPT Code: 84157
MERCY TEST NAME PROT TTL 24UR MERCY LAB CODE VPRT
5 mL of urine from an unpreserved 24-hour urine specimen that was refrigerate during
Specimen:
collection.
After 24 hours, store urine aliquots at 2–4°C for < 72 hours or frozen at -20°C for up to
Stability:
1 year.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: 0 - 150 mg/24 hours
Method
The Atellica CH UCFP assay is an adaptation of pyrogallol red-molybdate method.
Description:
CPT Code: 84156
POWERCHART PROTEIN RANDOM URINE
NAME
Patient A previous bone marrow transplant from an allogenic donor will interfere with testing. Call
Preparation: 800-533-1710 for instructions for testing patients who have received a bone marrow
transplant.
Specimen: 3 ml whole blood in an EDTA tube.
Processing: Send ambient in original tube. DO NOT ALIQUOT. Mayo order code (PTNT).
Coagulation Consultation Patient Information Sheet must be sent with specimen.
Performed: 3-5 days, weekly.
Reference Value: Included with test results
Method: Direct Mutation Analysis
CPT Code: 81240
POWERCHART PROTIME
NAME
Specimen: Draw a blue top tube (3.2% Citrate) filled appropriately with amount of blood listed on label.
Cause for
Improperly filled tubes will NOT be tested. Gross hemolysis unacceptable.
rejection:
Processing: Store refrigerated. Unopened, unspun tubes are stable 24 hours from time of collection. If
the order is for only a Protime, freeze if testing will not be done within 24 hours. Freeze
plasma if testing not done within 4 hours of collection if a PTT is also ordered. Label
frozen vial "CITRATED PLASMA". NOTE: Specimens for PTT MUST be removed from cells and
tested within 4 hours of collection or frozen.
Double spin coagulation specimens to ensure that all platelets are removed: 1. Centrifuge
specimen. Aliquot plasma (leaving some above the cells) to a plastic centrifuge tube. 2.
Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the tube) to
another plastic aliquot tube. 3. Store plasma as required for the test ordered.
Performed: Within 8 hours of receipt. Available stat.
Reference value: Protime INR Normal range (for patient not receiving anticoagulant): 0.8 - 1.2
Therapeutic range: Protime INR range: Indications:
2.0 - 3.0 Prophylaxis and treatment of venous
thrombosis
Treatment of pulmonary embolism
Prevention of systemic embolism
Tissue heart valves
Acute myocardial infarction
Valvular heart disease
Atrial fibrillation (valvular and nonvalvular)
INR range: 3.0 - 4.5 Indications:
Recurrent systemic embolism
Mechanical prosthetic valves
(recommendation currently under review)
POWERCHART PROTIME
NAME
MERCY TEST NAME PROTIME- POINT OF CARE MERCY LAB CODE PTR
Specimen: Fingerstick specimen obtained off first drop of blood or venous specimen collected in a non-
heparinized syringe.
Cause for Results greater than or equal to 6.0 will require a lab draw to be ran on the analyzer.
rejection: Clinic performed INR testing: Refer to the specific clinic procedure for the INR threshold that
will require a venipuncture specimen for analysis.
Reference value: Protime INR Normal range (for patient not receiving anticoagulant): 0.8 - 1.2
Method: Point of Care
CPT Code: 85610
POWERCHART PSA DIAGNOSTIC
NAME
Method The Atellica IM PSA assay is a 2‑site sandwich immunoassay using direct chemiluminescent
Description: technology, which uses constant amounts of 2 antibodies.
CPT Code: 84153
At least 12 months have passed following the month in which the last PSAS was performed.
Do not use specimens that have been stored at room temperature for longer than 8 hours.
Method The Atellica IM PSA assay is a 2‑site sandwich immunoassay using direct chemiluminescent
Description: technology, which uses constant amounts of 2 antibodies.
CPT Code: 84153
G0103
Specimen: 1.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send frozen to Mayo, (PSAFT).
Performed: 3 days. Monday through Saturday
Reference value: Included in report.
Method: Electrochemiluminescent Immunoassay (ECLIA)
POWERCHART PSEUDOCHOLINESTERASE
NAME
Specimen: 1.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Sent refrigerated to Mayo. Mayo order code (PCHE1).
Performed: 1-2 days, Monday through Sunday; continuously.
Included with test results.
Reference value:
MERCY TEST NAME PTT (Partial Thromboplastin Time) MERCY LAB CODE PTT
Specimen: Draw a blue top tube (3.2% citrate) filled appropriately with amount of blood listed on label.
Cause for
Improperly filled tubes will NOT be tested. Avoid gross hemolysis.
rejection:
Processing: In-house patients: Centrifuge immediately. Refrigerate. Test within 4 hours of collection.
if testing will be delayed longer than 4 hours. Double spin coagulation specimens to ensure
that all platelets are removed:
o Centrifuge specimen. Aliquot plasma (leaving some above the cells) to a plastic
centrifuge tube.
o Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the
tube) to another plastic aliquot tube.
o Store plasma in freezer. Label aliquot vial "CITRATED PLASMA."
o Centrifuge immediately.
o Aliquot specimen (leaving some above the cells) to a plastic centrifuge tube.
o Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the
tube) to another plastic aliquot tube.
o Send refrigerated if testing can be performed within 4 hours of collection.
o If testing will not be performed within 4 hours freeze specimen and send frozen.
o Label aliquot vial "CITRATED PLASMA."
Therapeutic 60-102 seconds. Applies only to PTT performed at MMC-NI using IL reagent SynthASil lot #
range: N0670635.
Method: Photo-optical clot detection.
CPT Code: 85730
MERCY TEST NAME RENAL (Kidney) FUNCTION PANEL MERCY LAB CODE RPNL
Stability: Serum may be stored for up to 7 days at 2–8°C or stored frozen for up to 30 days at
-20°C.
Comment: Includes: Albumin, Anion Gap, BUN, Bun/Creatinine Ratio, Calcium, CO2, Chloride, Creatinine,
eGFR, Glucose, Phosphorus, Potassium, Sodium
Thawed or frozen specimens which are turbid must be clarified by centrifugation prior to
testing.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: See individual test entry.
Method See individual test entry.
Description:
CPT Code: 80069
Comment: There can be 2 types of specimens drawn. Please check orders carefully as there are
different requirements for each specimen type.
non-venous specimens: Schedule with Radiology and indicate in the comment field if
specimen is to be other than venous collection. Consult Lab for patient preparation.
Venous specimens: Enter in comment field: venous specimen. Consult lab for patient
preparation.
Specimen: Non-venous: Lab is to draw in conjunction with radiology procedure. Need 2 ml plasma
from pre-chilled purple top tubes. Draw blood with chilled syringe, from a patient in a
seated position, place in chilled tube, mix immediately and place in an ice water bath until
thoroughly chilled.
Venous: Need 2 ml plasma from pre-chilled purple top tubes. Draw with a vacutainer from
patient in seated position.
Mix immediately and place in an ice water bath until thoroughly chilled.
Processing: Centrifuge in refrigerated centrifuge. Separate immediately and freeze. Indicate specimen
source on specimen tube and on order form. Send frozen to Mayo. Mayo order code PRA.
Mercy Lab Processing Note: Due to volumes showing that we are now only using a single
peripheral collection, the source has been hidden on the report and no longer needs to be
resulted. However, if a patient should require collections during a procedure that are non-
venous, the result of HIDE will need to be changed to the specific source of collection.
Comments: o Gram stain is done to assess sputum quality using the following criteria:
o >25 epithelial cells/low power field: The specimen is UNACCEPTABLE for
culture due to the large number of squamous epithelial cells present.
This is indicative of saliva. The specimen must be recollected for culture.
Nursing personnel will be notified by the laboratory.
o 11-25 epithelial cells/low power field: The specimen is probably a mixture
of lower respiratory secretions and saliva. The culture will be done, but
results may be unreliable.
o 0-10 epithelial cells/low power field: This is indicative of a good specimen.
Culture will be processed.
o Sinus Cultures are held for 7 days, and all bacterial growth is identified.
Susceptibility testing is done when possible.
o The gram stain report will also indicate the amount of epithelial cells seen, the
amount of WBCs, and any bacteria that may be present.
o Susceptibility testing will be routinely performed on significant isolates.
RL Client o Mark RESPIRATORY UPPER CULTURE/GRAM STAIN on order form.
Comments: Write collection site on SOURCE line.
If ordering in the computer, order test code RESP
Method: Standard culture techniques
Comment: This profile includes Immunoglobulin E, house dust mites DP, house dust mites DF, cat
epithelium, dog epithelium, Bermuda grass, timothy grass, cockroach, penicillium,
Cladosporium, Aspergillus fumigatus, Alternaria alternata, maple box elder, mountain cedar,
white oak, elm, walnut tree, eastern sycamore, cottonwood, white ash, pecan hickory,
mulberry, short ragweed, Russian thistle, rough pigweed, rough marsh elder
Specimen: 4.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Minimum volume calculation: (0.05ul x # of allergens) + 0.25 ul
Processing Send refrigerated to Mayo.
Mayo test order code (RPR8)
Performed: 1-5 days. Test set up Monday through Friday.
CPT Code:
86003 x26
Comment: Ordered by Blood Bank personnel only. Will be ordered by Blood Bank personnel when RH
IMMUNE GLOBULIN WORKUP indicates eligibility for RH Immune Globulin.
MERCY TEST NAME RHIG LOT # (RH IMMUNE GLOBULIN LOT#) MERCY LAB RHG
CODE
NOTE: If the mother's type is unknown, an ABO Group/Rh Type should be ordered prior to
ordering RHIG. Order in the following conditions on Rh negative mothers: Per physician's
order when the RHIG injection only is ordered prenatally or following miscarriage,
amniocentesis, or after any event which may allow fetal cells to enter the mother's
circulation. If the physician also orders an antibody screen, order Antibody Screen.
Specimen: No specimen needed.
Performed: Within 8 hours of receipt.
CPT Code: NA
Comment: Order on the mother after delivery. A Cord Blood Routine must be ordered on the neonate.
Includes: ABO/RH and Direct Coomb's (DAT) results for Cord Blood Routine on the neonate.
If mother is eligible for Rh immune globulin injection, Lab will order RHIG Eligibility Studies.
Specimen: None.
Performed: Within 8 hours of receipt.
Method: Decisional to establish eligibility of mother to receive RHIG.
CPT Code: NA
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo test order code (RNP)
Performed: 1-2 days. Performed Monday through Saturday; 4 p.m.
Reference Value: Included with report
Method: Multiplex Flow Immunoassay
CPT Code: 86235
Specimen: Minimum of 1 gm of a random stool specimen submitted in a clean container with a tight-
fitting lid. Deliver to the laboratory immediately after collection. Send to Mercy Lab
refrigerated. Specimen is stable 72 hours refrigerated.
Method: EIA
Note: Do not order this test on patients greater than 18 years old.
Comment: o Test is very specimen dependent. False negatives may be reported if the
specimen is inadequate or poorly collected.
o If Pertussis by PCR is also ordered, collect the Pertussis PCR swabs first.
o Although testing is always available whenever a diagnosis of RSV is suspected,
testing for RSV is not recommended outside of the respiratory virus season or in
the absence of an outbreak due to low specificity of the test.
RL Client o Order test code RSVS or mark RSV ANTIGEN on order form.
Comments:
Method: EIA
The performance of the assay has not been established for populations of
immunocompromised or immunosuppressed patients.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: Immune
Within 8 hours of receipt
Method The Atellica IM Rub G assay is a sandwich immunoassay using direct chemiluminescent
Description: Technology.
CPT Code: 86762
o AmniSure should not be used earlier than 6 hours after the removal of any
disinfectant solution or medicines from the vagina.
Cellular debris may potentially interfere with sample preparation.
o Specimens should not be obtained from patients with suspected or known placenta
previa.
o Intended for use in patients with gross bleeding.
Regional Lab: Refrigerate if not tested within 30 minutes. Must be tested within 6 hours.
Performed: Within 6 hours of receipt. Available STAT
Reference value: Included with report.
Method: Rapid Non-instrumented qualitative immunochromatography
CPT Code: 84112
POWERCHART
ANTI SCLERODERMA (SCL-70)
NAME
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo order code (SCL70)
Performed: 1-2 days. Monday through Saturday; 4 p.m.
Reference value: Included in report
Method: Multiplex Flow Immunoassay
CPT Code: 86235
POWERCHART
SED RATE - ERYTHROCYTE
NAME
Specimen: On Campus; preferred specimen: 1.5 ml whole blood collected in purple top EDTA tube or
500 mcl collected in MAP capillary tube. Draw 2 tubes if CBC and ESR are ordered. Do not
draw in pink top EDTA tubes.
Processing: Refrigerated specimen best if run within 24 hours but will be accepted up to 36 hours.
Performed: Within 8 hours of receipt. Available stat
Reference value: AGE MALE FEMALE
1-30 days 0-2 0-2 MM/HR
30 days - 11 yr. 3-13 3-13 MM/HR
12 - 49 years 0 - 15 0 - 20 MM/HR
> 49 years 0 - 20 0 - 30 MM/HR
Method: Automated, ISED analyzer NOTE: Results from the ISED are not affected by low patient HCT.
Therefore, it may be necessary to establish a new patient baseline.
CPT Code: 85652
Specimen: o Draw before any other tubes are drawn. 0.8 ml serum from Navy blue monoject-
no additive, trace element blood collection tube.
o Use alcohol, not iodine to cleanse venipuncture site.
Processing: o Allow to clot well (for at least 30 minutes before spinning). Then centrifuge the
specimen to separate serum from the cellular fraction. Serum must be removed
from the cells within 4 hours of specimen collection. Pour serum into a
Mayo Metal FREE vial. Do NOT use a transfer pipet or wooden sticks.
o Send to Mayo refrigerated. Ambient acceptable. Mayo order code (SES)
Performed: 1-3 days. Monday through Saturday.
Reference value: Included in report
Method: Dynamic Reaction Cell-Inductively Coupled Plasma-Mass Spectrometry (DRC-ICP-MS)
CPT Code: 84255
Specimen: o Draw before any other tubes are drawn. 0.8 ml whole blood from metal free Royal
blue top EDTA additive, blood collection tube.
o Use alcohol, not iodine to cleanse venipuncture site.
Processing: o Send specimen in original tube.
o Send to Mayo refrigerated. Ambient and frozen are acceptable. Mayo order code
(SEWB)
Performed: 1-7 days. Monday
Reference value: Included in report
Method: Inductively Coupled Plasma Mass Spectrometry
CPT Code: 84255
TEST NAME SEMEN ANALYSIS FERTILITY See: Fertility Test Semen (RL Clients ONLY)
Note: For Semen Analysis from Reference Lab Clients please refer to Fertility Test Semen.
Comment: Specimen accepted Monday-Thursday only, not the day before a holiday, until 8 PM
nightly.
Mayo courier picks up specimens at Mercy after 8PM. Specimen should be collected as close
to shipping time as possible. If ONLY a sperm count is ordered, see SPERM COUNT.
Includes: Semen analysis includes description of Appearance, Ph, Volume, Sperm Count, Motility
Evaluation and Sperm Morphology.
Specimen: Semen specimen collected in Semen Collection Kit provided by Mayo. Patient is to deliver
the specimen, packed in the collection kit, to Mercy Lab within 1 hour of collection. For
accurate results, the patient should have 2-7 days of sexual abstinence prior to specimen
collection. It is critical to keep specimen at room temperature.
Method: Includes color, volume, viscosity, pH, % motility, concentration, grade of motility, viability,
morphology, and presence of cellular elements.
TEST NAME SEX CHROMATIN See: Cytology Section Barr Body Smear
POWERCHART
NAME SEX HORMONE BINDING GLOBULIN
MERCY TEST NAME SEX HORM BIND GLOB* MERCY LAB CODE SHBG
Specimen: 1 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo order code (SHBG1)
Performed: 1-3 days. Monday through Friday; 5 a.m. - 3 p.m., Saturday; 6 a.m. - 3 p.m.
Reference value: Included in report
Method: Immunoenzymatic Assay
CPT Code: 84270
POWERCHART SIROLIMUS (RAPAMYCIN) LEVEL
NAME
When a Sirolimus and Tacrolimus are ordered on the same patient 2 tubes must be
collected, one for each test.
Processing: Send specimen in original collection tube. Send Refrigerated to Mayo. Mayo order
code SIIRO
Performed: Daily
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo order code (SM)
Performed: 1-2 days. Monday through Saturday; 4 p.m.
Reference value: Included in report
Method: Multiplex Flow Immunoassay
CPT Code: 86235
Method The methods for measurement of electrolytes include flame photometry, spectrophotometry
Description: and direct or indirect ion selective electrode potentiometry.
The A‑LYTE Na, K, and Cl assays use indirect Integrated Multisensor Technology (IMT). There
are four electrodes used to measure electrolytes. Three of these electrodes are ion-selective
for sodium, potassium, and chloride. A reference electrode is also incorporated in the
multisensor.
CPT Code: 84295
POWERCHART SODIUM 24 HOUR URINE
NAME
MERCY TEST NAME SODIUM 24 HOUR URINE MERCY LAB CODE VNA
Method The methods for measurement of electrolytes include flame photometry, spectrophotometry
Description: and direct or indirect ion selective electrode potentiometry.
The A‑LYTE Na, K, and Cl assays use indirect Integrated Multisensor Technology (IMT). There
are four electrodes used to measure electrolytes. Three of these electrodes are ion-selective
for sodium, potassium, and chloride. A reference electrode is also incorporated in the
multisensor.
CPT Code: 84300
MERCY TEST NAME SOD POT 24UR MERCY LAB CODE VLYT
Method The methods for measurement of electrolytes include flame photometry, spectrophotometry
Description: and direct or indirect ion selective electrode potentiometry.
The A‑LYTE Na, K, and Cl assays use indirect Integrated Multisensor Technology (IMT). There
are four electrodes used to measure electrolytes. Three of these electrodes are ion-selective
for sodium, potassium, and chloride. A reference electrode is also incorporated in the
multisensor.
CPT Code: 84300 Sod Urine +
84133 Pot Urine +
Method The methods for measurement of electrolytes include flame photometry, spectrophotometry
Description: and direct or indirect ion selective electrode potentiometry.
The A‑LYTE Na, K, and Cl assays use indirect Integrated Multisensor Technology (IMT). There
are four electrodes used to measure electrolytes. Three of these electrodes are ion-selective
for sodium, potassium, and chloride. A reference electrode is also incorporated in the
multisensor.
CPT Code: 84295 Sodium
84132 Potassium
Comment: This is also the test to be ordered when checking for sperm after a vasovasostomy
procedure- slides will come from surgery. Under comment: enter Vasovasostomy and
source. Obtain specimen on a slide and place in the surgery pass through. Notify the Lab
that a specimen is there.
Specimen: Semen specimen collected in a clean plastic container. Deliver to the Lab within 12 hours of
collection. The specimen should not be collected or delivered in a condom. For accurate
results, the male should not ejaculate semen for a minimum of two days prior to specimen
collection.
MERCY TEST NAME SSA ARO AB IGG* MERCY LAB CODE SSAB
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo order code (SSA)
Performed: 1-2 days. Monday through Saturday; 4 p.m.
Reference value: Included in report
Method: Multiplex Flow Immunoassay
CPT Code: 86235
POWERCHART
SJOGREN'S ANTIBODIES (SSA/SSB)
NAME
MERCY TEST NAME SSA AND SSB AB IGG MERCY LAB CODE SSABB
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Frozen is also acceptable.
Mayo order code (SSAB).
Performed: 1-3 days. Test set up Monday through Saturday; 4 p.m.
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo order code (SSB)
Performed: 1-3 days. Monday through Saturday; 4 p.m.
Reference value: Included in report
Method: Multiplex Flow Immunoassay
CPT Code: 86235
MERCY TEST NAME STOOL PATHOGENS PCR MERCY LAB CODE STLPCR
Also acceptable: Unpreserved liquid or soft stool specimen in a dry, clean container.
MercyOne lab staff will transfer unpreserved stool sample immediately into a Cary-Blair preservative
container for possible serotyping of any positive result on receipt.
Performed: Daily, 0900 cut off time for morning run and 1900 cut off time for evening run
MERCY TEST NAME STREP PNEUM AG, UR* MERCY LAB CODE SPNAU
MERCY TEST NAME STREP PNEUMO AB* MERCY LAB CODE PN23
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or a plain red top tube.
MERCY TEST NAME STREP SCRN THRT MERCY LAB CODE GAS
Specimen: Collect the specimen from the tonsils and pharynx using 2 swabs. Submit in a double
Culturette.
Comments: o A throat culture will be ordered and charged by lab personnel on all negative Rapid
Strep Screens in order to detect very low numbers of beta strep Group A and other
significant beta streptococci.
o This test is very specimen dependent. False negative results may be reported if the
specimen is inadequate or poorly collected.
RL Client
o Write GROUP A STREP SCREEN on the order form.
Comments:
o Send Culturette at room temperature.
Performed: Within 8 hours of receipt.
Method: EIA
Comment: oThis order screens for colonization of Vancomycin Resistant Enterococcus only.
o Contact the microbiology lab if other sites are being submitted to look for VRE.
Performed: Preliminary report: 2 days.
MERCY TEST NAME VRE SRV CLT / NON-MERCY MERCY LAB CODE VREX
Comment: o This order screens for colonization of Vancomycin Resistant Enterococcus only.
o Contact the microbiology lab if other sites are being submitted to look for VRE.
o Send specimen at room temperature.
o Write VRE Screen or VRE Surveillance on the order form.
o This testing will be billed back to the ordering facility.
Performed: Preliminary report: 2 days.
Final report: 3 days.
MERCY TEST NAME SYPHILIS TOTAL ANTIBODY MERCY LAB CODE SYPHT
WITH REFLEX
Performance of this assay has not been established with neonatal specimens.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: 2.5 - 3.9 pg/ml
Method The Atellica IM FT3 assay is a competitive immunoassay using direct chemiluminescent
Description: technology.
CPT Code: 84481
POWERCHART T3 TOTAL
NAME
Comment: Do not use samples that have been stored at room temperature for longer than 8 hours.
Performance of this assay has not been established with neonatal specimens.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: 0.89-1.76 ng/mL
Method The Atellica IM FT4 assay is a competitive immunoassay using direct chemiluminescent
Description: technology.
CPT Code: 84439
POWERCHART T4 TOTAL
NAME
MERCY TEST NAME T&B CELL QN* MERCY LAB CODE TBCL
Patient For 12 hours before specimen collection do not take multivitamins or dietary supplements
Preparation: containing biotin (vitamin B7), which is commonly found in hair, skin and nail supplements
and multivitamins.
Specimen: 3 mL whole blood from a (purple top) EDTA. Minimum 1.0 mL.
When a Tacrolimus and Sirolimus are ordered on the same patient 2 tubes must be
collected, one for each test.
Processing: Send specimen in original collection tube. Sent refrigerated to Mayo. Mayo order code
(TAKRO).
Performed: Daily
Reference value: Included in report.
Method: High-Pressure Liquid Chromatography/Tandem Mass Spectrometry (HPLC-MS/MS)
CPT Code: 80197 - Tacrolimus
POWERCHART QUANTIFERON TB GOLD IN-TUBE
NAME
Specimen: Special collection kit, QuantiFERON - TB Gold In-Tube collection kit. Kit Includes: Collection
directions, 4 tubes, QTB Transport bag. Kit is stored in MercyOne Laboratory. Lab Aide's rack
shelf A5.
o Collect 1 mL of blood in each of the 4 tubes. When the tube is upright, blood must
meet the small black mark on label.
o Tubes fill slowly.
o If butterfly needle is used, first collect other required tubes or use a "purge" tube
to remove the air and then proceed with collecting the QTB tubes.
o Shake tubes firmly for 10 times (entire inner surface of tube must be coated with
blood)
o Overly energetic shaking may cause gel disruption and could lead to aberrant
results.
o Label tubes appropriately
o Maintain tubes at room temperature until incubation portion of test preparation is
started.
INCUBATION OF TUBES NEEDS TO BE PERFORMED WITHIN 16 HOURS OF INITIAL
COLLECTION OF TUBES
Cause for Improper collection, incubation, centrifugation, or storage of specimens is cause for
rejection: rejection
Processing: Collect kit specifically as directed, incubate tubes 16-24 Hr., centrifuge and store as directed
in instructions. Send Refrigerated to Mayo Medical Laboratories Mayo code (QFT4).
Specimen: Spin no additive serum tube and remove from red cells 1.0 mls of serum. No other tube
type acceptable.
Processing: Send refrigerated to Mayo. Mayo order code (TTST).
Patients’ age and sex are required on requisition for processing.
MERCY TEST NAME TESTOST TTL FRE* MERCY LAB CODE TSTF
Specimen: Spin no additive serum tube and remove from red cells 2.5 mls of serum. No other tube
type acceptable.
Processing: Send refrigerated to Mayo. Mayo order code (TGRP).
Performed: Monday through Sunday. Free Testosterone determination may take 10 days.
Reference value: Included with report.
Method: Liquid Chromatography – Tandem Mass spectrometry (LC-MS/MS)
Equilibrium Dialysis
CPT Code: 84403 Testosterone, Total
84402 Testosterone, Free
MERCY TEST NAME THRT CLT STREP MERCY LAB CODE THSC
Specimen: Collect the specimen with a double swab Culturette. Rub the sterile swabs firmly over the
back of the throat (posterior pharynx), both tonsils, and any areas of inflammation. Submit
the specimen as soon after collection as possible. If there is a delay in transport, the
specimen should be forwarded within 48 hours of collection. The specimen should be stored
and sent at room temperature.
Specimen: Draw blue top tube filled with amount of blood listed on label.
Cause for
Gross hemolysis. Improperly filled tubes will not be tested.
Rejection:
Processing: Centrifuge immediately. Stable 4 hours at room temperature. Test heparin containing
specimens within 2 hours. DO NOT FREEZE SPECIMEN.
Performed: Within 8 hours of receipt, available stat.
Reference value: 13.0-17.0 seconds
Method: Photo-optical clot detection.
CPT Code: 85670
Specimen: 3.0 mL whole blood EDTA tube. Yellow top ACD tube and sodium citrate are also acceptable.
AND
6.0 mL platelet poor plasma from light-blue top (citrate) tube. (Requires at least 6 blue-
top tubes)
Both whole blood and plasma are required.
Draw enough citrated whole blood to spin down and aliquot 6.0 mL platelet poor
plasma in 6 plastic vials, each containing 1 mL
Draw enough citrated whole blood to spin down and aliquot 6.0 mL platelet poor plasma in
6 plastic vials, each containing 1 mL.
Spin down, remove plasma, and spin plasma again. Remove plasma and place in plastic
aliquot vials. Freeze specimens immediately at
< or = -40 degrees C, if possible. Label specimens as plasma.
Shipping
Send plasma frozen and whole blood ambient. Mayo order code (AATHR).
Instructions:
Specimen: 1 ml serum from a no additive serum tube. A Serum Separator Tube (SST) is NOT acceptable.
Processing: Send refrigerated. Mayo order code TGAB. If Thyroglobulin Tumor Marker testing is desired
and ordered, DO NOT order this test. Order a Thyroglobulin Tumor Marker which includes
both Thyroglobulin and Thyroglobulin antibody.
Performed: Test set up Monday through Friday 6 AM - 12 AM, Saturday 6 AM - 6 PM.
Reference value: Included with results.
Method: Immunoenzymatic Assay
CPT Code: 86800
Specimen: 1 ml serum from a no additive serum tube. A Serum Separator Tube (SST)
is NOT acceptable.
Processing: Send refrigerated to Mayo. Mayo order code (HTG2). DO NOT order a Thyroid Antibody
Screen with Thyroglobulin Tumor Marker. The screen is included in Tumor Marker testing
and a separate order would be considered duplicate testing.
Performed: Test set up Monday through Saturday
Reference value: Included with results.
Method: Thyroglobulin Mass Spectrometry
CPT Code: 86800 Thyroglobulin Antibody Screen
84432 Thyroglobulin Tumor Marker
MERCY TEST NAME THYRD HYPER PNL MERCY LAB CODE THPE
Do not use samples that have been stored at room temperature for longer than 8 hours.
Performance of this assay has not been established with neonatal specimens.
Performed:
Within 8 hours of receipt. Available stat.
Reference Range: See individual test entry.
Method
See individual test entry.
Description:
CPT Code: 84439 T4 Free
84481 T3 Free
POWERCHART THYROID HYPO PANEL (TSH SENSITIVE AND FREE T4)
NAME
MERCY TEST NAME THYRD HYPO PNL MERCY LAB CODE THPO
Do not use samples that have been stored at room temperature for longer than 8 hours.
Performance of this assay has not been established with neonatal specimens.
Method
Description: See individual test entry.
CPT Code: 84439 T4 Free
84443 TSH
MERCY TEST NAME THYROID STIM IMGLB* MERCY LAB CODE THYIMG
Specimen: 0.5 ml serum from a no additive serum tube or Serum Separator Tube (SST).
Processing: Send frozen to Mayo. Mayo order code (TSI).
Performed: 2-6 days. Monday - Friday 10 AM
Reference Value: Included with results
Method: Recombinant Bioassay
CPT Code: 84445
Specimen: 1 mL serum from a Serum Separator Tube (SST) (Preferred). Serum from a no additive
serum tube is also acceptable.
Patient For 12 hours before specimen collection do not take multivitamins or dietary supplements
Preparation: containing biotin (vitamin B7), which is commonly found in hair, skin, nail supplements and
multivitamins.
Processing: Send refrigerated (Preferred) to Mayo. Frozen is also acceptable. Mayo order code (THYRO).
Specimen: Aseptically place the specimen in a sterile plastic container with a tight-fitting lid. The
specimen should be surgically obtained. If unable to transport to the laboratory promptly,
add 1 - 2 ml of sterile saline to the specimen container.
RL Client
o Write TISSUE CULTURE on the order form. Indicate the specimen source.
Comments:
o Send specimen at room temperature.
Performed: Preliminary reports: Days 1-4
Final report: 5 days
Specimen: Tissue specimen covered with 10% Formalin. Transport containers and 10% formalin are
available from the Laboratory.
Performed: 2 days.
Reference value: Interpretation will be provided.
Method: Pathologist evaluation.
CPT Code: Varies.
MERCY TEST NAME TISSUE TRANSGLUT AB IGA MERCY LAB CODE TTA
Specimen: 1.0 ml of serum from a no additive serum tube or Serum Separator Tube (SST). Refrigerated
Cause for
Hemolysis.
rejection:
Comment: This new assay performs the Tissue Transglutaminase and deamidated Gliadin
simultaneously for IgA. There is no need for a separate order for the tTG and deamidated
gliadin. Ordering the TTA will cover for both assays, simultaneously, but will be reported as
one result for IgA.
Processing: Specimen can be refrigerated for up to 21 days. Send refrigerated to Mayo. Frozen is
acceptable. Mayo order code TTGG.
Performed: Monday thru Saturday at Mayo
Reference Value: Included with the report
Method: EIA
CPT Code: 86364
MERCY TEST NAME TISSUE TRANSGLUT AB* MERCY LAB CODE TISTA
Specimen: 1.0 ml of serum from a no additive serum tube or Serum Separator Tube (SST). Refrigerated
Cause for
Hemolysis.
rejection:
Comment: This new assay performs the Tissue Transglutaminase and deamidated Gliadin
simultaneously for IgA and simultaneously for IgG. There is no need for a separate order for
the tTG and deamidated gliadin. Ordering the TISTA will cover for both assays,
simultaneously, but will be reported as one result for IgA and one result for IgG
Processing: Specimen can be refrigerated for up to 21 days. Send refrigerated to Mayo. Frozen is
acceptable. Mayo order code TSTGP.
Performed: Monday thru Saturday at Mayo
Reference Value: Included with the report
Method: EIA
CPT Code: 86364 x 2.
Specimen: 1 ml serum from a plain, no additive serum tube. Serum gel/SST are not acceptable.
Processing: Send refrigerated (Preferred) to Mayo. Ambient or Frozen also acceptable. Mayo order code
(TOPI).
Includes: This testing includes Mayo Medical Lab's Torch Profile IgG, Toxoplasma gondii Antibody, IgM
and Cytomegalovirus (CMV) Antibodies, IgM (Mayo TRCHG, TXM and CMVM).
TRCHG: HSV Type 1 and Type 2 Ab IgG, Toxoplasma Ab IgG, Rubella Ab IgG, Cytomegalovirus
Ab IgG
TXM: Toxoplasma gondii Antibody, IgM
CMVM: Cytomegalovirus (CMV) Antibodies, IgM
This testing should be ordered as a Miscellaneous General Chemistry. Indicate that testing is
for TORCH.
This test is to be ordered only on neonates, pregnant women or women who have had
miscarriages.
If the patient does not meet any of these qualifications, then each test must be ordered
separately.
MERCY TEST NAME TOXIC VOLATILE SCRN MERCY LAB CODE TVS
Comment: Complete and send to Lab a Toxic Volatile Screen Patient Information Sheet.
Specimen: 1 Spun Serum Separator Tube (SST). Send refrigerated.
1 no additive serum tube, spun, aliquoted and labeled as no additive serum tube. Send
refrigerated
1 gray top Sodium Fluoride. Send refrigerated
1 small dark green top lithium heparin tube completely filled and on ice.
Use aqueous betadine for cleaning venipuncture site, not alcohol swab.
Processing: 1.0 ml serum from orange or gold tube for metabolic panel and osmolality. Perform alcohol
testing upon first opening. Keep small green top tube closed and on ice for venous pH. Plain
red top tube and gray top tube are used only if confirmatory tests are indicated.
Reference value: Refer to individual test entry and Toxic Volatile Screen Laboratory Results for Frequent
Situations table which follows on next page. Mercy technical staff, refer to Osmolality
procedure for analysis, calculations, & interpretation.
MERCY TEST NAME TOXOPLASMA IGG, IGM* MERCY LAB CODE TOXOGM
POWERCHART TRANSFERRIN
NAME
Comment: MMC-NI Nursing Services should notify the physician and Lab immediately and follow
Nursing Policy #602 Blood Transfusion Reaction Investigation which can be found on the
Mercy Intranet Home page, Policies, Procedures and By-Laws, Nursing. In this policy under
the procedure portion, A.5. follow the helpful link to Documentation Guidelines: Blood
Transfusion Reaction. Nursing will fill out "Post Transfusion" documentation form in
Powerchart selecting "YES" in the Transfusion Reaction box and any other required fields.
Once "YES" is selected the "Transfusion Reaction Workup" will be automatically ordered and
generated to the Laboratory.
Nursing should continue with the Transfusion Reaction by delivering the Lab copy of the
Blood/Blood Component Transfusion Form and the blood/component bag with all attached
tubing and IV solutions to the Lab immediately. Continue to monitor patient. There is no
charge to the patient.
Comment: For use by Emergency Center ONLY and only in a trauma situation.
Includes: Alcohol, blood Amylase CBC with Diff
Metabolic Panel Protime PTT
Specimen: Draw a Serum Separator Tube (SST), a blue top sodium citrate tube filled appropriately with
amount of blood listed on label, 1 purple top (EDTA) tube, 1 pink top tube, and 1 gray top
Sodium Fluoride tube on ice for a possible lactic acid.
Performed: Within 8 hours of receipt. Available stat.
Reference value: See individual test entry.
Method: See individual test entry.
CPT Code: 85025 CBC
85610 Protime
85730 PTT
80048 Basic Metabolic Pnl
82150 Amylase
G0480 / 82077 Alcohol Ethyl Bld
Comment: To be ordered by Lab on any specimen collected by Lab personnel outside the Laboratory
facility.
CPT Code: P9604
Specimen: Urine: First void urine specimens must be transferred from the collection cup to the Xpert
Urine Transport Reagent Tube (Yellow Cap) immediately (preferred) or within 4 hours of
collection when kept at Room Temperature or within 4 days of collection when stored at 2–
8°C.
Urine specimens in Xpert Urine Transport Reagent Tubes should be kept between 2°C and
30°C during transport and can be stored for up to 14 days at this temperature.
o The patient should collect the first 20–60 mL of voided urine (the first part of the
stream - not midstream) into a urine collection cup.
o Cap and label the urine collection cup with patient identification and date/time
collected.
Vaginal/Endocervical/: Collect using only the Xpert Swab Specimen Collection Kit (Pink
Cap). Swab samples in Xpert Swab Transport Reagent are stable up to 60 days at 2–30°C.
POWERCHART TRIGLYCERIDES
NAME
Reference Range:
No established reference range available.
Method The Atellica CH Trig_2 assay is based on an enzymatic procedure in which a combination of
Description: enzymes is employed for the measurement of serum or plasma triglycerides.
CPT Code: 84478
POWERCHART T3 REVERSE
NAME
Specimen: 0.8 ml serum from a no additive serum tube or a Serum Separator Tube (SST).
Performed: 2-6 days. Test set up Monday, Wednesday, Friday: 8:30 a.m.
Processing: Send refrigerated to Mayo. Mayo order code (RT3).
Reference value: Included in report.
Method: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT Code: 84482
POWERCHART TROPONIN I
NAME
Comment: The use of a single sample type (either lithium-heparin plasma or serum) is recommended
for troponin analysis when collecting serial samples from the same patient.
For serum specimens, complete clot formation should take place before centrifugation.
Serum should be physically separated from cells as soon as possible from the time of
collection.
Method The Atellica IM TnIH is a 3-site sandwich immunoassay using direct chemiluminescent
Description: Technology.
CPT Code: 84484
POWERCHART TRYPTASE
NAME
Specimen: 0.5 mL serum from a Serum Separator Tube (SST-preferred), or a no additive serum tube-
acceptable
Processing: Aliquot specimen, send FROZEN to Mayo. Mayo order code (TRYPT).
Method The Atellica IM TSH3‑UL assay is a third-generation assay that employs anti-FITC monoclonal
Description: antibody covalently bound to paramagnetic particles, an FITC-labeled anti-TSH capture
mouse monoclonal antibody, and a tracer consisting of a proprietary acridinium ester and
an anti‑TSH mouse monoclonal antibody conjugated to bovine serum albumin (BSA) for
chemiluminescent detection.
CPT Code: 84443
All patients drawn for possible blood product transfusion MUST be correctly identified
and MUST BE WEARING an armband with their FULL NAME and MEDICAL RECORD
NUMBER before the patient is drawn.
A (check mark) must be put by the Medical Record number on the tubes drawn for
a Type and Screen by the person drawing the specimen indicating the phlebotomist
has matched the medical record number on the Specimen with the medical record
number on the Patient Armband and it is identical along with the name and other
pertinent information.
Date, time, and initials of the individual collecting the specimen must be on the tube.
POWERCHART
UREA BREATH TEST
NAME
MERCY TEST NAME UREA BREATH TEST MERCY LAB CODE HPUBT
Patient Patient cannot eat or drink (including chewing gum) 1 hour prior to testing. Must be able to
Preparation: swallow a solution and blow-up balloon. For Further directions please see the Patient
Preparation and Specimen Collection Procedure Located in the Special Helps Section.
Processing: Bag of breath must be full. Send specimen ambient. Mayo order code UBT.
It is not available to inpatients due to the extensive preparation of discontinuing medication.
Comment:
Testing for Helicobacter Pylori
Performed: Monday through Friday; 6:30 a.m. - 4:30 p.m.
Reference value: Included with report.
Method: Infrared Spectrophotometry (SP)
CPT Code: 83013
POWERCHART
UREA NITROGEN 24 HOUR URINE
NAME
MERCY TEST NAME UREA NITROGEN 24UR MERCY LAB CODE VUN
Specimen: 5 mL of urine from an unpreserved 24-hour urine specimen kept refrigerate during
collection.
Urine urea nitrogen may be stored for up to 4 days at 4–8°C when preserved with thymol to
Stability:
avoid bacterial action.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: 12 - 20 g/24 hours
Method The Atellica CH Urea Nitrogen (UN_c) assay is based on the Roch-Ramel enzymatic reaction
Description: using urease and glutamate dehydrogenase.
CPT Code: 84540
POWERCHART
Urea Nitrogen Random Urine
NAME
MERCY TEST NAME Urea Random Urine MERCY LAB CODE RUREA
POWERCHART
UREA CLEARANCE 24 HOUR URINE
NAME
0.5 mL serum and 5 mL urine from an unpreserved 24-hour urine specimen kept
Specimen:
refrigerate during collection.
Separated blood urea nitrogen is stable in separated serum or plasma and may be stored
Stability: for up to 3–5 days at room temperature or for up to 7 days at 4°C or stored frozen
indefinitely at -20°C.
Urine urea nitrogen may be stored for up to 4 days at 4–8°C when preserved with thymol
to avoid bacterial action.
Includes: Volume (ml/24 hours) Raw Urea Nitrogen (mg/dl)
Calc. Urea Nitrogen (g/24 hours) Urea Nitrogen Clearance (ml/min)
Comment: Outpatients and Inpatients: MercyOne Laboratory will order and draw the appropriate
no charge serum BUN (BUNNC) if a serum BUN has not been completed within 48 hours.
Regional Lab Clients: MercyOne Laboratory will order the serum BUN at no charge. Do
not order a single BUN on the requisition.
POWERCHART
URIC ACID
NAME
Method The Atellica CH Uric Acid (UA) assay is based on the Fossati enzymatic reaction using uricase
Description: with a Trinder-like endpoint.
CPT Code: 84550
POWERCHART
URIC ACID 24 HOUR URINE
NAME
MERCY TEST NAME URIC ACID 24UR MERCY LAB CODE VURI
Specimen: 5 mL urine from an unpreserved 24-hour urine specimen kept refrigerate during collection.
Stability: Specimens may be stored for 3–4 days at ambient temperature for alkaline urine.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: 250 - 750 mg/24 hours
Method The Atellica CH Uric Acid (UA) assay is based on the Fossati enzymatic reaction using uricase
Description: with a Trinder-like endpoint.
CPT Code: 84560
POWERCHART
URIC ACID BODY FLUID
NAME
POWERCHART
UA URINALYSIS COMPLETE
NAME
Includes: Appearance, Glucose, Occult Blood, Urobilinogen, Bilirubin, Ketones, Ph, Leukocytes,
Protein, Nitrites, Specific Gravity.
A microscopic description of the specimen will be included.
**This order is for lab generated order only. The order is NOT available in Cerner
Powerchart.
Specimen: Recommend testing volume: 15 mL of freshly voided urine.
4 mL minimum (adults)
2 mL minimum (infant/pediatric)
Deliver specimen to lab as soon as possible after collection.
First morning specimen is preferred for testing, but random collections are
acceptable.
Midstream:
Instruct patient of the proper collection technique. Collect in a sterile plastic container with
a tight-fitting lid. Provide the patient with 3 antiseptic towelettes.
Use the following collection procedure:
-The patient should thoroughly wash their hands.
-Remove the lid from the container. Do not touch the inside surfaces.
-Remove all clothing from waist down.
-Assume the appropriate position.
-Female: Sit on the toilet seat with legs spread apart.
-Male: Stand facing the toilet or sit on the toilet with legs spread apart.
-Open the towelettes and cleanse perineal area.
-Female: Separate the labia with the thumb and forefinger. Using downward strokes,
cleanse one labia with a towelette and discard. Cleanse the other labium and meatus in the
same fashion, using a separate towelette for each stroke, and discard. Keep the labia
separated.
-Male: If uncircumcised, retract the foreskin before proceeding. Cleanse the head of the
penis with a towelette, using a circular motion from the urethral opening to the outer
diameter of the penis. Discard towelette. Repeat using all the towelettes.
-Hold container by the outside surface. Begin urinating into the toilet.
-Place container under the stream of urine after a good flow has started.
-Fill container half full and void remainder of the urine into the toilet.
-Screw on the sterile cover. Do not touch the inner surface.
Cause for Unpreserved specimens >2 hours at room temperature or >8 hours refrigerated.
rejection:
Comment: Indicate time of collection in comment. Indicate method of collection using the following
codes:
MURN Midstream
CURN Cath
SURN Suprapubic
Performed: Within 2 hours of receipt. Available Stat.
Includes: Appearance, Glucose, Occult Blood, Urobilinogen, Bilirubin, Ketones, Ph, Leukocytes,
Protein, Nitrites, Specific Gravity.
A microscopic description of the specimen will be included on hazy and cloudy specimens
and those specimens having one or more positive results on the dipstick except Glucose and
Ketone. Clear urines with negative dipsticks will not have the microscopic analysis
performed.
Comment: Indicate time of collection in comment. Indicate method of collection using the following
codes:
MURN Midstream
CURN Cath
SURN Suprapubic
A microscopic exam is performed and charged if any of the following exist:
Clarity is hazy, cloudy, or turbid.
All positive Chemstrip results except for positive Glucose or positive Ketones.
Providers may specifically request a microscopic be performed by writing “Urinalysis with
Micro” on the requisition.
Performed: Within 2 hours of receipt. Available Stat.
POWERCHART
URINE CULTURE
NAME
Midstream:
Instruct patient of the proper collection technique. Collect in a sterile plastic container with a
tight-fitting lid. Provide the patient with 3 antiseptic towelettes. Use the following collection
procedure:
In-dwelling catheter:
Obtain the specimen with a needle and syringe. Select a puncture site 1-2 inches distal to the
meatus. Clean the area to be punctured with 70% alcohol. Aspirate 10 ml of urine with a
sterile needle and syringe.
NOTE: Specimens obtained from the collection bag are NOT clinically useful. FOLEY
TIPS WILL NOT BE ACCEPTED.
ALL OUTSIDE CLIENTS (INCLUDING NURSING HOMES)
1. Fill the urine transport tube with the urine specimen (about 4 ml).
2. If there is <4 ml of urine, remove the rubber stopper from the tube and
fill it to the minimum mark with urine. Replace the rubber stopper and
mix well.
3. If the specimen was collected from an in-dwelling catheter using a
syringe, inject the needle through the rubber stopper and allow the
vacuum inside of the tube to draw the correct volume into the tube.
4. Transport at room temperature. Specimen must be received by
Microbiology Lab within 48 hours of collection.
o Results will be quantitated in colony forming units/ml.
o Specimens containing more than 3 organisms will NOT routinely have organism
identifications or susceptibility testing reported. This is generally indicative of an
Comments:
improperly collected specimen.
o Foley catheter tips will NOT be cultured.
o Susceptibility testing will be routinely performed on all significant isolates.
o Urine culture transport tubes are not acceptable for urinalysis.
Performed: Final report: 1 - 2 days
POWERCHART
URINE DIPSTICK
NAME
Includes: Appearance, Glucose, Occult Blood, Urobilinogen, Bilirubin, Ketones, Ph, Leukocytes,
Protein, Nitrites, Specific Gravity.
Cause for Specimens >2 hours at room temperature or >8 hours refrigerated.
rejection:
Comment: Indicate time of collection in comment. Indicate method of collection using the following
codes:
MURN Midstream
CURN Cath
SURN Suprapubic
Performed: Within 2 hours of receipt. Available Stat.
Reference Value: Spec. Gravity: 1.001-1.035
Ph: 4.6-8.0
Protein: Negative
Glucose: Negative
Ketones: Negative
Bilirubin: Negative
Occult Blood: Negative
Urobilinogen: Negative
Leukocytes: Negative
Nitrites: Negative
Method: Reagent strip.
POWERCHART
URINE MEASUREMENT
NAME
Comment: To be ordered by the Lab on any urine specimen measured by Mercy Lab personnel.
Method: Manually using a graduated cylinder or container.
CPT Code: 81050
POWERCHART
URINE MICROSCOPIC ONLY
NAME
MERCY TEST NAME URINE MICRO ONLY MERCY LAB CODE UCM
First morning specimen is preferred for testing, but random collections are
acceptable.
Midstream:
Instruct patient of the proper collection technique. Collect in a sterile plastic container with
a tight-fitting lid. Provide the patient with 3 antiseptic towelettes.
POWERCHART
VALPROIC ACID (DEPAKENE) LEVEL
NAME
Specimen: 1.0 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
This test is for immune status.
For diagnosis of recent infection, testing of IgM and IgG antibodies are recommended
Comment:
(VZGM)
Alias: Chicken Pox
Processing: Specimen stability is 14 days when refrigerated. Send refrigerated to Mayo. Mayo order
code VZPG.
MERCY TEST NAME VARIC ZOST IgG IgM* MERCY LAB VZGM
CODE
Specimen: 1 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Comment: Includes IgG and IgM results
Processing: Send in screw-capped, round bottom, plastic vial, refrigerated to Mayo. Mayo order code
(VZGM).
Alias: Chicken Pox
Performed: Test set up Monday through Saturday
Reference value: Included with Results
Method: IgG: Multiplex Flow Immunoassay (MFI)
IgM: Immunofluorescence Assay (IFA)
MERCY TEST NAME VARIC ZST, NOT BLD* MERCY LAB LVZV
CODE
Specimen: Must indicate specimen source. Submit only 1 of the following specimens:
Body fluid (Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular) – 0.5 mL of
fluid in a sterile container. Do not centrifuge. Send to Mayo in Sarstedt Aliquot Tube, 5
mL (T914).
Swab (Miscellaneous; dermal, eye, nasal, or throat) – Culturette (BBL Culture Swab)
(T092). Send swab to Mayo in multimicrobe media (M4-RT, M4 or M5).
Genital Swab (Cervix, vagina, urethra, anal/rectal, or other genital sources) – Culturette
(BBL Culture Swab) (T092). Send swab to Mayo in multimicrobe media (M4-RT, M4 or M5).
Respiratory (Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or
washing, sputum, or tracheal aspirate) – 1.5 mL of specimen in sterile container.
Tissue (Brain, colon, kidney, liver, lung, etc.) – Entire collection in a Multimicrobe media
(M4-RT) (T605) (Preferred) or Sterile container with 1–2 mL of sterile saline.
Cautions: A negative result does not exclude the possibility of varicella-zoster virus (VZV)
infection. The reference range is typically “negative” for this assay. This assay is only to be
used for patients with a clinical history and symptoms consistent with VZV infection and
must be interpreted in the context of the clinical picture. This test should not be used to
screen asymptomatic patients.
Processing: Send refrigerated to Mayo. Frozen is also acceptable. Mayo order code (VZVPV).
MERCY TEST NAME VASC ENDO GRW FCTR* MERCY LAB VEGF
CODE
o Immediately after specimen collection, place the tube on wet ice.
Collection
o Centrifuge at 1500 x g for 10 minutes and aliquot plasma into plastic vial.
Instructions:
o Freeze specimen within 30 minutes.
0.5 mL plasma from a Lavender-top (EDTA) tube.
Specimen:
Aliquoted plasma MUST be frozen within 30 minutes of collection
Processing: Send frozen to Mayo. Mayo order code (VEGF).
Specimen: 1.0 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo order code (VASC).
Performed: Monday through Saturday
Reference value: Reference ranges included with results.
Method: Multiplex flow immunoassay.
POWERCHART VISCOSITY
NAME
3.0 ml serum from a no additive serum tube, serum gel/SST are NOT acceptable. Keep
Specimen:
specimen at 37°C until after centrifugation and separation of cells.
Processing: Send to Mayo. Mayo order code (SVISC).
Performed: 1-3 days. Test set up Monday through Friday; continuously until 2 p.m.
Reference value: Included in report.
Method: Benson BV200 capillary method
CPT Code: 85810
POWERCHART VITAMIN A (Retinol) LEVEL
NAME
Specimen: 0.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Collection: o Specimen must be collected following an overnight (12-14 hr.) fast.
o Infants - draw prior to next feeding.
Processing: Send serum refrigerated to Mayo Medical Laboratories. Mayo order code (VITA).
MERCY TEST NAME THIAMIN VIT B1* MERCY LAB CODE VB1
Cause for
Specimens other than whole blood.
Rejection:
Performed: Within 6-11 days from receipt at Mayo Labs.
Reference ranges included with result
Reference value:
Method: High Performance Liquid Chromatography
CPT Code: 84425 Vitamin B1
1 mL plasma heparin from a sodium or lithium heparin or plasma gel separator tube (PST).
Specimen:
**Protect from light**
Patient Prep: Patient must be fasting overnight (12-14 hours) (infants-collect prior to next feeding)
Patient must not ingest vitamin supplements for 24 hours before specimen collection
Centrifuge at 4 degrees C within 2 hours of collection, then aliquot all plasma
Processing:
into amber vial. Send to Mayo Frozen. Mayo Code - (B6PRO)
Performed: 1 - 7 days. Monday through Thursday, Sunday
Reference value: Reference ranges included with result
Method: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
82542
CPT Code:
84207
Do not use samples that have been stored at room temperature for longer than 8 hours.
Comment:
Intrinsic Factor (Mayo test IFBA) will be reflex ordered and charged on all specimens with a
B12 less than 180 pg/mL.
Preservatives, such as fluoride and ascorbic acid, interfere with the Atellica IM VB12 assay.
180-914 pg/mL
Reference Range: Indeterminate Range: 146 - 179 pg/mL
Deficient Range: 0 - 145 pg/mL
The Atellica IM VB12 assay is a competitive immunoassay using direct chemiluminescent
Method
Technology.
Description:
CPT Code: 82607 Vit B12
MERCY TEST NAME VIT B12 FOLATE MERCY LAB CODE B12F
Specimen: 0.5 ml serum
Comment: Collect before blood transfusion. Folate should not be ordered for patients who have
recently received a radioisotope, methotrexate, or other folic acid antagonist. If adequate
amount of specimen and the result is below the reference range.
Folate reference range based on populations with folic acid fortification of foods. Deficient
folate concentrations are considered to be less than 4ng/mL.
Intrinsic Factor (Mayo test IFBA) will be reflex ordered and charged on all specimens with a
B12 less than 180 pg/mL.
Do not use samples that have been stored at room temperature for longer than 24 hours.
Performed: Within 8 hours of receipt. Available stat.
Reference Range: 10-24 ng/mL (mild to moderate deficiency)
25-80 ng/mL (optimum levels)
>80 ng/mL (toxicity possible)
Method The Atellica IM VitD assay is a competitive immunoassay that uses an anti-fluorescein mouse
Description: monoclonal antibody covalently bound to paramagnetic particles
CPT Code: 82306
MERCY TEST NAME 25 HYDROXY VD2 D3* MERCY LAB CODE 25HDN
Comment: The test will report out three results, 25-Hydroxy D2, 25-Hydroxy D3, and 25-Hydroxy Total
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Serum stable 14 days refrigerated.
Processing: Specimen is to be sent refrigerated. Mayo order code (25HDN).
Performed: 2-5 days.
Reference Value: Included in report.
MERCY TEST NAME 1,25 DIHYDR VTMN D* MERCY LAB CODE DHVD
Specimen: 1.5 mL serum from a Serum Separator Tube (SST) or no additive serum tube.
Collection
Fasting (4-hour preferred but not required)
Instructions:
Processing: Send serum refrigerated to Mayo. Mayo order code (DHVD)
Performed: 2-4 days. Monday through Friday; 3 p.m.
Reference
Included with test results.
Value:
Method: Extraction/Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
CPT Code: 82652
Specimen: Add 25 mL 50% acetic acid preservative at the start of the collection. If specimen is
refrigerated during collection, preservative may be added up to 4 hours after collection. This
preservative is intended to achieve a pH of between approximately 1 and 5. If necessary,
adjust urine pH to 1 to 5 with 50% acetic or HCl acid. Patient's age and 24-hour volume
required.
Reference Lab Adjust pH to 1.0-5.0 with 50% acetic acid. Aliquot 20 ml and indicate the 24-hour volume.
Processing: Separate aliquots must be submitted for Metanephrines and Catecholamines if collected
with this specimen. Identify which specimen is for VMA. Mayo order code (VMA).
MERCY TEST NAME VON WILL FACT AG* MERCY LAB CODE VONW
Specimen: 1 ml plasma from blue top tube filled appropriately with amount of blood listed on the label.
Processing: Centrifuge, remove plasma, spin plasma again, aliquot to a new plastic tube. Freeze plasma
in plastic vial. Send frozen to Mayo. Order Von Willebrand Antigen Mayo test code (VWAG).
Double spin coagulation specimens to ensure that all platelets are removed: 1. Centrifuge
specimen. Aliquot plasma (leaving some above the cells) to a plastic centrifuge tube. 2.
Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the tube) to
another plastic aliquot tube. 3. Store plasma as required for the test ordered.
MERCY TEST NAME PROFILE VON WILL* MERCY LAB CODE VWPR
Specimen: 3 mL plasma from blue top tube filled appropriately with the amount of blood listed on the
label.
Aliquot 3 mL of platelet poor plasma in 3 plastic vials each containing 1 mL.
Processing: Centrifuge, remove plasma, spin plasma again, aliquot to a new plastic tube. Freeze plasma
in plastic vial. Send frozen to Mayo. Order Mayo (AVWPR).
Double spin coagulation specimens to ensure that all platelets are removed:
1. Centrifuge specimen. Aliquot plasma (leaving some above the cells) to a plastic centrifuge
tube.
2. Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the tube) to
another plastic aliquot tube.
MERCY TEST NAME WEST NILE, IgG & IgM* MERCY LAB
WNILE
CODE
Specimen: 0.5 ml serum from a Serum Separator Tube (SST) or no additive serum tube.
Processing: Send refrigerated to Mayo. Mayo order code (WNS).
Performed: 1-4 days. Monday - Friday: 9 a.m. (June-October)
Monday, Wednesday, Friday; 9 a.m. (November-May)
Reference Value: Included in report
MERCY TEST NAME WBC AND AUTOMATED DIFF MERCY LAB WBCADI
CODE
WBC and automated differential (include absolute neutrophil counts). Manual differential is
Includes:
done if indicated by test results.
Comment: Cell morphology will be ordered and charged if established criteria/diagnosis are met.
Specimen: 1 purple top (EDTA) tube.
Processing: Specimen stable 36 hours at either room temperature or refrigerated.
Performed: Within 8 hours of receipt. Available stat.
Reference value: Included with test results. Complete listing in Special Helps section of Lab Test Index.
Method: Automated cell counter.
CPT Code: 85048 WBC
85004 AUTOMATED DIFFERENTIAL
POWERCHART WBC COUNT
NAME
Specimen: Disinfect the skin. If possible, aspirate purulent material with a sterile needle and syringe.
Transfer this material to a sterile plastic container with a tight-fitting lid. If an aspirate is not
possible, collect purulent material from a deep area of the wound/abscess on a culture swab
device (that contains 2 swabs).
Specimen stability: Aspirate: 24 hours room temperature. Culture Swab: 48 hours room
temperature.
Comment: o If anaerobic organisms are suspected, collect a second specimen. See Anaerobic
Culture/Gram Stain for collection and ordering instructions.
o Susceptibility testing will routinely be performed on significant isolates.
RL Client o Mark WOUND CULTURE on order form. Write collection site on SOURCE line.
Comments: If ordering in the computer, use order code WNDC.
o Send specimen at room temperature.
Performed: Gram stain: Within 8 hours of receipt.
Preliminary report: Days 1 and 2
Final report: 3 days
If testing criteria is met, IDPH will fax a test request form for the provider to fill out. This form
includes patient history. THIS FORM MUST ACCOMPANY ANY SAMPLE(s) SENT TO MERCY
LAB.
Processing: Mercy Lab: send to State Hygienic Lab, refrigerated. Place the urine in a biohazard bag, place
the serum in a biohazard bag and then place both
of those in another biohazard bag (double bagged). Place the form in the outside pocket of the
biohazard bag. Place that biohazard bag into the clear Ziploc CDS bag. Follow the CDS send out
procedure for scheduling a pickup.
Processing: o Allow to clot well (for at least 30 minutes before spinning). Then centrifuge the
specimen to separate serum from the cellular fraction. Serum must be removed from
the cells within 4 hours of specimen collection. Pour serum into a Mayo Metal Free
vial. Do NOT use a transfer pipet or wooden sticks. Avoid hemolysis.
o Send to Mayo refrigerated. Ambient and frozen are acceptable. Mayo order code
(ZN_S).
Performed: 1-3 Days. Monday through Saturday.
Reference Values: included with report
Method: Dynamic Reaction Cell Inductively Coupled Plasma Mass Spectrometry (DRC-ICP-MS)
CPT Code: 84630