Phytohormones

www.plantcell.org/cgi/doi/10.1105/tpc.110.tt0310
 What are phytohormones?

“........characterized by the
property of serving as chemical
messengers, by which the
activity of certain organs is
coordinated with that of others”.
-Frits Went and Kenneth Thimann, 1937

Frits Went, 1903-1990 Kenneth Thimann, 1904-1997

Frits Went image courtesy of Missouri Botanical Garden ©2010 Kenneth Thimann photo courtesy of UC Santa Cruz
 Phytohormones
Phytohormones regulate cellular activities (division, elongation and
differentiation), pattern formation, organogenesis, reproduction, sex
determination, and responses to abiotic and biotic stress.

Notholaena standleyi © 2008 Carl Rothfels

Phytohormones – old timers and
newcomers
Cytokinins Gibberellins
Auxin
Abscisic Acid

Ethylene

Strigolactones
Brassinosteroids
Salicylates Jasmonates
 Phytohormones regulate all
stages of the plant life cycle
Fruit Germination
ripening
Seed
Embryogenesis dormancy
Growth and
branching

Fertilization and Flower

fruit formation development
 Hormones also help plants cope
with stress throughout their life
Fruit Germination
ripening
Seed
Embryogenesis dormancy
Growth and
branching

Fertilization and Flower

fruit formation development
Most hormones affect most stages of
the plant life cycle

We will examine each hormones within the

context of one of its roles.

Remember that these are merely examples;

most hormones affect most processes in
one way or another.
Lecture outline
How hormones work
Hormonal control of vegetative development
Auxin
Cytokinins
Strigolactones
Gibberellins
Brassinosteroids
Hormonal control of reproduction
Ethylene
Abscisic Acid
Hormonal responses to stress
Salicylates
Jasmonates
Cross-regulation of hormonal effects
Hormones: Synthesis, transport,
perception, signaling and responses
Downstream
effects

Production of
active hormone

Transport

Downstream

H
effects

Binding to
receptor Signal
transduction
 Synthesis
Conjugation
H
H
De-conjugation
Many tightly regulated
biochemical pathways
Synthesis Breakdown
Production of contribute to active
active hormone hormone accumulation.
Conjugation can
temporarily store a
hormone in an inert form,
lead to catabolic
breakdown, or be the
means for producing the
active hormone.
Transport and perception

Production of
active hormone Hormones can move:
• through the xylem or phloem
• across cellular membranes
Transport • through regulated transport proteins

H Several hormone receptors

have recently been identified.
Binding to They can be membrane
receptor bound or soluble
 Signal transduction
Hormonal signals are
transduced in diverse
ways. Common
methods are
reversible protein
phosphorylation and
targeted proteolysis Protein
phosphorylation

H
Protein
dephosphorylation

Proteolysis

Signal
transduction
Responses
Downstream
Downstream effects can effects
involve changes in gene
transcription and changes
in other cellular activities
like ion transport

Transcription

Downstream
effects
Non-genomic effects
(e.g. Ion channel
regulation)
 Hormones: Synthesis, transport,
perception, signaling and responses
H
Conjugation Downstream

H
De-conjugation effects

Synthesis Breakdown
Production of
active hormone

Transcription
Protein
Transport phosphorylation

P Downstream

H
Protein
effects
dephosphorylation
Non-genomic effects
Proteolysis (e.g. Ion channel
Binding to regulation)
receptor Signal
transduction
Receptors can be membrane-bound

Ethylene Cytokinins Brassinosteroids

Hormone binding initiates an information relay

 Soluble receptors can facilitate
interactions between proteins
Hormones can act like “molecular glue”
TIR1
Auxin

PYR1
ABA

GA
COI1 JA-
GID1 Ile
 Some receptors initiate protein
proteolysis

The hormones (red) bind to

receptors (green), initiating
proteolysis of repressors
(yellow) to activate a
transcriptional regulator
(blue)

Auxin Gibberellin Jasmonate

Reprinted by permission from Macmillan Publishers, Ltd: NATURE Wolters, H., and Jürgens, G. (2009). Survival of the flexible:
Hormonal growth control and adaptation in plant development. Nat. Rev. Genet. 10: 305–317. Copyright 2009.
Proteolytic targets are covalently
linked to ubiquitin
Ubiquitin
Ubiquitin is a small (76 aa)
protein that targets proteins for
Target proteolytic cleavage.

Ubiquitin by Rogerdodd
Ubiquitin ligase complexes
ubiquitinate target proteins
TIR1
Auxin Ubiquitin is
ligated to the
target

The auxin and jasomonate

receptors are F-box proteins,
part of an SCF ubiquitin
ligase complex

TIR: TRANSPORT INHIBITOR RESPONSE 1

Ubiquitinated proteins are
targeted for proteolysis

26S proteasome
Hormones affect vegetative growth: elongation,
branching and organogenesis

Elongation in the shoot and

root of a germinating soybean
Organogenesis

Germinated
seedling Growth by
branching

Growth by
elongation
Photo courtesy of Shawn Conley
Disrupting hormone synthesis or
response interferes with elongation
GA Auxin Brassinosteroid
Pea Arabidopsis Arabidopsis

Wild type Gibberellin Wild type Auxin Wild type Brassinosteroid

biosynthesis response biosynthesis
mutant mutant mutants
Lester, D.R., Ross, J.J., Davies, P.J., and Reid, J.B. (1997) Mendel’s stem length gene (Le) encodes a gibberellin 3-hydroxylase. Plant Cell 9: 1435-1443.;Gray WM
(2004) Hormonal regulation of plant growth and development. PLoS Biol 2(9): e311; Clouse SD (2002) Brassinosteroids: The Arabidopsis Book. Rockville, MD:
American Society of Plant Biologists. doi: 10.1199/tab.0009
 Auxin

•Growth
•Phototropism and gravitropism
•Branching
•Embryonic patterning
•Stem cell maintenance
Indole-3-acetic acid (IAA), the
•Organ initiation most abundant natural auxin
Auxin controls growth

Charles Darwin studied

the way seedlings
bend towards light, a Site of signal
direct effect of auxin perception

action

Site of
response

Coleoptile drawing from Darwin, C., and Darwin, F. (1881) The power of movement in plants. Available online.
Darwin concluded that a signal
moves through the plant controlling
growth
“We must therefore conclude that when
seedlings are freely exposed to a lateral
light some influence is transmitted from
the upper to the lower part, causing the
latter to bend.”

Coleoptile drawing from Darwin, C., and Darwin, F. (1881) The power of movement in plants. Available online. Photograph of Darin statue by Patche99z
Differential cell growth is a result of
auxin movement to the shaded side

Auxin
Cell accumulation
length on shaded side
stimulates
elongation and
bending.
Auxin
concentration

Esmon, C.A. et al. (2006) A gradient of auxin and auxin-dependent transcription precedes tropic growth responses. Proc. Natl. Acad. Sci. USA 103: 236–241.
Friml, J., et al. (2002) Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis. Nature 415: 806-809.
Auxin moves in part by a
chemiosmotic mechanism
Cell wall
pH 5.5
Auxin is a charged anion (IAA-) in
Cytoplasm the cytoplasm (pH 7).
pH 7

IAA- In the more acidic cell wall (pH 5.5)

some is uncharged (IAAH). The
uncharged form crosses the
IAAH IAA- + H+ plasma membrane into the cell
where it is deprotonated and
unable to exit other than through
IAAH
specific transporters.

IAA- + H+

Redrawn from Robert, H.S., and Friml, J. (2009) Auxin and other signals on the move in plants. Nat. Chem. Biol. 5: 325-332.
Polar auxin transport
Cell wall
pH 5.5
Auxin transport out of cells is controlled
Cytoplasm by three families of transport proteins that
pH 7
collectively control the directionality of
IAA- auxin movement. Asymmetric distribution
of the transporters controls polar auxin
transport.
IAAH IAA- + H+

IAAH
Net flow
of auxin
IAA- + H+

Redrawn from Robert, H.S., and Friml, J. (2009) Auxin and other signals on the move in plants. Nat. Chem. Biol. 5: 325-332.
PIN: PIN FORMEDs
Pgp: P-glycoprotein) (ABCB1)
AUX1/LAX: AUX/LAXAUXIN RESISTANT/ LIKE AUXIN RESISTANT
Col-0 pin1
 Auxin biosynthesis
Indole
IAA is produced from
tryptophan (Trp) via several
semi-independent pathways and Tryptophan
one Trp-independent pathway.
Indole-3-
Environmental and
pyruvic acid Tryptamine Indole-3- Indole-3-
developmental control of the (IPA) acetamide acetaldoximine
genes controlling auxin (IAM) (IAOx)
biosynthesis, conjugation and
degradation maintain auxin Indole-3-
acetaldehyde
homeostasis.

IAA

Adapted from Quittenden, L.J., Davies, N.W., Smith, J.A., Molesworth, P.P., Tivendale, N.D., and Ross, J.J.
(2009). Auxin biosynthesis in pea: Characterization of the tryptamine pathway. Plant Physiol. 151: 1130-1138..
Auxin regulates plant development
Lateral organ initiation at the
shoot apical meristem
Inhibit branching in
the shoot

Patterning and vascular

development

Maintain stem cell fate Promote branching

at the root apical in the root
meristem

Wolters, H., and Jürgens, G. (2009). Survival of the flexible: Hormonal growth control and adaptation in plant development. Nat. Rev. Genet. 10: 305–317.
Many of auxin’s effects are mediated
by changes in gene expression
Genes controlling
cell growth

Genes involved in
signaling

Genes coordinating other

hormone response
pathways

Wolters, H., and Jürgens, G. (2009). Survival of the flexible: Hormonal growth control and adaptation in plant development. Nat. Rev. Genet. 10: 305–317.
The auxin signaling pathway

Aux/IAA

ARF
SCFTIR1
IAA
IAA Aux/IAA

1. Auxin binds to SCFTIR1

and Aux/IAA
The auxin signaling pathway
2. Aux/IAA
ubiquitinated and
degraded by 26S
Aux/IAA
proteasome
ARF
SCFTIR1
IAA
IAA

1. Auxin binds to SCFTIR1

and Aux/IAA
The auxin signaling pathway
2. Aux/IAA
ubiquitinated and
degraded by 26S
Aux/IAA
proteasome
ARF
SCFTIR1
IAA
IAA
3. Degradation of
1. Auxin binds to SCFTIR1 repressor permits
and Aux/IAA transcriptional
activation by ARF
transcription
ARF factors
Cytokinins

•Cell division
•Control of leaf
senescence
•Control of nutrient
allocation
•Root nodule
development
•Stem cell maintenance
•Regulate auxin action trans-zeatin, a cytokinin
Cytokinins are a family of related
adenine-like compounds

dihydrozeatin cis-zeatin
Isopentenyl adenine trans-zeatin

Hirose, N., Takei, K., Kuroha, T., Kamada-Nobusada, T., Hayashi, H., and Sakakibara, H. (2008). Regulation of cytokinin
biosynthesis, compartmentalization and translocation. J. Exp. Bot. 59: 75–83.
 Cytokinin (CK) biosynthesis
Regulated
by CK and
nitrogen
Tissue specific;
auxin, CK and CK biosynthesis
ABA sensitive and inactivation are
strongly regulated
Meristem by CK, other
specific hormones and
exogenous factors.

Inactive form

CKX
Upregulated by
CK and ABA

Hirose, N., Takei, K., Kuroha, T., Kamada-Nobusada, T., Hayashi, H., and Sakakibara, H. (2008). Regulation of cytokinin
biosynthesis, compartmentalization and translocation. J. Exp. Bot. 59: 75–83.
Cytokinins act antagonistically to auxins
CK Auxin
Promote
Promote stem Promote lateral branching in
cell fate at the organ initiation the shoot
shoot apical at the shoot Inhibit
meristem apical branching in
meristem the shoot

Inhibit
Promote Maintain stem Promote branching in
differentiation cell fate at the branching the root
at the root root apical in the root
apical meristem
meristem
Reprinted by permission from Macmillan Publishers, Ltd: NATURE Wolters, H., and Jürgens, G. (2009). Survival of the flexible:
Hormonal growth control and adaptation in plant development. Nat. Rev. Genet. 10: 305–317. Copyright 2009.
 Auxin and cytokinin regulate each
other’s function at the root apex
Auxin
Cell Through effects on each other’s synthesis,
transport transport and response, auxin and cytokinin
differentiation
establish two mutually exclusive domains that
coordinate cellular activities at the root apex.
Cell
division Cytokinin

Auxin transport Cytokinin

and response biosynthesis

Auxin
Auxin, cytokinin and strigolactones
control branching
Root branches,
called lateral roots,
are promoted by
auxin and inhibited
by CK

Branching controls
every aspect of plant
productivity from
Shoot branches are nutrient uptake to
promoted by CK crop yields.
and inhibited by
auxin and
strigolactones
Coleus shoot image by Judy Jernstedt, BSA ; lateral root image from Casimiro, I., et al. (2001) Auxin
transport promotes Arabidopsis lateral root initiation. Plant Cell 13: 843-852.
Cytokinin signaling is mediated by
a two-component system
Input Transmitter Receiver Output
domain domain domain domain

H D

Histidine Kinase Response Regulator

A two-component system is a short signaling pathway that moves

information from an input to an output. In bacteria it usually consists of
two proteins, a histidine kinase (HK) with a conserved histidine residue
(H) and a response regulator (RR) with a conserved aspartate residue
(D).
Information and phosphoryl groups
are relayed between the components
ATP ADP

P P
H D

Histidine Kinase Response Regulator

Stimulation of the input domain activates the kinase activity,

phosphorylating the transmitter domain. The phosphoryl group is
subsequently relayed to the response regulator.
 Two component signaling in plants

H D H D
Hybrid histidine kinase Histidine Response
phosphotransferase regulator
(HPt)

Most plant HKs are hybrid histidine kinase, which

transfer the phosphoryl group to a histidine
phosphotransferase. The HPt transfers it to a
response regulator.
 Cytokinin control of gene expression in
Arabidopsis
Receptor

AHK2/3/4 H H

D D Type B Arabidopsis response regulators (ARRs)

are transcription factors. Type A or C ARRs
HPt H interfere with CK signaling through as yet
unknown means.
D
A-ARR or
C-ARR D
B-ARR
Transcription
Cytokinins affect grain production
and drought tolerance
Rice plants that
accumulate more CK
can produce more
grain per plant
because of changes in
inflorescence
architecture.

Wild-type Elevated CK
Tobacco plants that produce
more CK are more drought
tolerant because of the delay
in leaf senescence conferred
by CK.

Ashikari, M. et al. (2005) Cytokinin oxidase regulates rice grain production. Science 309: 741 – 745, with
permission from AAAS; Rivero, R. M. et al. (2007) PNAS 104: 19631-19636.
Strigolactones

Strigolactones, synthesized from carotenoids,

are produced in plant roots. They attract
mycorrhizal fungi and promote the germination
of parasitic plants of the genus Striga.
Image source USDA APHIS PPQ Archive ; Reprinted from Tsuchiya, Y., and McCourt, P. (2009). Strigolactones: A new
hormone with a past. Curr. Opin. Plant Biol. 12: 556–561 with permission from Elsevier.
 Strigolactones inhibit branch
outgrowth WT Mutant
Auxin transported from
the shoot to the root
Apex induces strigolactone
synthesis, which indirectly
inhibits bud outgrowth.
Bud

In a rice mutant that

does not produce
strigolactones, tillers
(lateral branches)
Auxin Strigolactone grow out as shown.

Lin, H., et al. (2009) DWARF27, an iron-containing protein required for the biosynthesis of strigolactones,
regulates rice tiller bud outgrowth. Plant Cell 21: 1512-1525.
Gibberellins

•Growth
•Seed germination
•Promote flowering
•Promote sex
determination in some
species
A Gibberellin (GA4)
•Promote fruit growth
 Gibberellins are a family of
compounds
GA4 is the major active
GA in Arabidopsis

Only some GAs are

biologically active. The
major bioactive gibberellins
are shown here.

Sun T (2008) Gibberellin metabolism, perception and signaling pathways in Arabidopsis: September 24, 2008. The Arabidopsis Book. Rockville,
MD: American Society of Plant Biologists. doi: 10.1199/tab.0103
Major GA biosynthetic and catabolic
pathways in higher plants

Active

Inactivation

Olszewski, N., Sun, T.p., and Gubler, F. (2002) Gibberellin Signaling: Biosynthesis, Catabolism, and Response Pathways Plant Cell 14: S61-S80
Gibberellins regulate growth

The pea mutant le, studied by

Mendel, encodes GA3 oxidase, which
produces active GA. Loss of function
of le reduces active GA levels and
makes plants dwarfed.

Active

Inactivation

Wild type Gibberellin

biosynthesis
mutant le
Lester, D.R., Ross, J.J., Davies, P.J., and Reid, J.B. (1997) Mendel’s stem length gene (Le) encodes a gibberellin
3-hydroxylase. Plant Cell 9: 1435-1443.
 Genes controlling GA synthesis are
important “green revolution” genes
Tremendous increases in crop
yields (the Green Revolution)
during the 20th century
occurred because of increased
use of fertilizer and the
introduction of semidwarf
varieties of grains.

The semidwarf varieties put

more energy into seed
production than stem growth,
and are sturdier and less likely
to fall over.
Distinguished plant breeder and Nobel Laureate
Norman Borlaug 1914-2009
Photos courtesy of S. Harrison, LSU Ag center and The World Food Prize.
Several of the green revolution
genes affect GA biosynthesis
Wild-type Semidwarf

Active

Semidwarf rice varieties

underproduce GA because
of a mutation in a the GA20
oxidase biosynthetic gene.

Reprinted by permission from Macmillan Publishers, Ltd. (Nature) Sasaki, A., et al. (2002) Green revolution: A mutant gibberellin-synthesis gene in rice Nature 416: 701-
702, copyright 2002.; Olszewski, N., Sun, T.p., and Gubler, F. (2002) Gibberellin sgnaling: Biosynthesis, catabolism, and response pathways Plant Cell 14: S61-S80.
 GA signaling pathway
Low GA High GA
Growth
GA-responsive promotion
transcription
factor
GA
DELLA protein

DELLA
Transcription
No
GA receptor transcription

DELLA proteins inhibit growth, in

part through blocking
transcription. GA triggers DELLA Proteolysis
protein proteolysis.
Reprinted from Davière, J.-M., de Lucas, M., and Prat, S. (2008) Transcription factor interaction: a central step in DELLA function. Curr. Opin. Genet. Devel. 18:
295–303.with permission from Elsevier
 A gene affecting DELLA protein
stability is a “green revolution” gene
Wild-type The wheat Rht1 locus encodes
a DELLA protein. The dwarf
allele lacks the DELLA domain
and resists proteolysis.
GA
Dwarf rht1
Rht1
DELLA
Transcription rht1
GA

rht1

Reprinted by permission of Macmillan Publishers, Ltd. Peng, J., et al. (1999) 'Green
revolution' genes encode mutant gibberellin response modulators. Nature 400: 256-261.
Brassinosteroids

•Cell elongation
•Pollen tube growth
•Seed germination
•Differentiation of
vascular tissues and Brassinolide, the
root hairs most active
brassinosteroid
•Stress tolerance
Brassinosteroid (BR) mutants are
dwarfed
Arabidopsis BRs promote cell elongation
in part by loosening cell walls

Tomato

Pea Cell wall

loosening

Lowered resistance to internal

turgor pressure; cell expansion

Bishop, G. J., and Koncz, C. Brassinosteroids and plant steroid hormone signaling. (2002) Plant Cell14: S97-S110.
 Reducing BR signaling produces
dwarf barley
H
H
The uzu plants have a
missense mutation in the
Wild-type BR receptor, making them
uzu less sensitive to BR. This is
the first dwarf grain
produced through
modification of BR
Cell Less cell signaling.
elongation elongation

Chono, M., et al., (2003) A semidwarf phenotype of barley uzu results from a nucleotide substitution in
the gene encoding a putative brassinosteroid receptor Plant Physiology 133:1209-1219.
 BR signaling
Low BR Without BR, the
receptor (BRI1) is
Receptor bound to an inhibitor
(BRI1) (BKI1). The active
Inhibitor BIN2 kinase
(BKI1) phosphorylates and
inactivates
transcription factors.
BIN2 P

P
P
 BR signaling
Low BR High BR BR-binding causes
BAK1 and the BRI1
Receptor receptor to
(BRI1) phosphorylate each
BAK1
Inhibitor other and BSKs.
(BKI1) P P BSKs phosphorylate
and active BSU1
phosphatase, which
BIN2 P inactivates BIN2.
BSKs P When BIN2 is
BSU1 P
inactive, its target
P transcription factors
P BIN2
are
dephosphorylated
and active.

Transcription
Summary – hormonal control of
vegetative growth
Plant hormones have diverse effects
on plant growth.

Auxin, gibberellins and

brassinosteroids contribute to
elongation growth.

Auxin, cytokinins and strigolactones

regulate branching patterns.

Growth and branching profoundly

affect crop yields.
Hormonal control of reproductive
development
In angiosperms:
• transition from vegetative to
reproductive growth
• flower development,
• fruit development and
ripening
• seed development,
maturation and germination

Photo courtesy of Tom Donald

Transition to flowering
The decision to reproduce is tightly
controlled by environmental and
hormonal factors. For many plants day-
length is critical in this transition, but
other plants are day-length neutral.
Similarly, some plants absolutely require
specific hormonal signals which have
little or no effects on other plants.

Lithops flowering
 GA’s role in initiating flowering varies
by species and growth-habit

Lolium temulentum Beta vulgaris Malus domestica Arabidopsis thaliana

Annual temperate grass Biennial Perennial Annual
Yes Yes No Short Days Long Days
Yes No

Photos courtesy of Plate 271 from Anne Pratt's Flowering Plants, Grasses, Sedges and Ferns of Great Britain c.1878,
by permission of Shrewsbury Museums Service; David Kuykendall ARS; Vincent Martinez; Takato Imaizumi.
 Ethylene promotes flowering in
pineapples and other bromeliads

A pineapple is a fruit
produced from
pineapple flowers.
Commercial growers
treat the plants with
ethylene to
synchronize flowering.

Images couresy of Dave McShaffrey, Marietta College, ©2009, used by permission.

Flower development
Hormones contribute to
flower development in
many ways:
• Patterning of the floral
meristem
• Outgrowth of organs
• Development of the
male and female
gametophytes
• Cell elongation
Ethylene and gibberellins are
involved in sex determination

Hermaphrodite Male Female

Image courtesy of Abdelhafid Bendahmane, URGV - Plant Genomics Research INRA

Fruit development and ripening
are under hormonal control

Pollination initiates petal

senescence, cell division
and expansion in the
ovary to produce a fruit,
and fruit ripening.
 Auxin and GA promote cell
division and growth of the fruit
Seedless varieties of grapes
and other fruits require
exogenous application of GA for Auxin + GA
fruit development. Strawberry
receptacles respond to auxin.

GA

Auxin

Photo credits: Grape flowers by Bruce Reisch; Strawberry flower by Shizhao

Fruit ripening is induced by
ethylene

Auxin Ethylene
GA

Ethylene is a gaseous hormone

that promotes fruit softening and
flavor and color development
 Ethylene
•Control of fruit ripening
•Control of leaf and
petal senescence
•Control of cell division
and cell elongation C2H4
•Sex determination in C2H4

some plants
•Control of root growth
•Stress responses Ethylene induces the triple response:
•reduced elongation,
H H •hypocotyl swelling,
C C •apical hook exaggeration.
H H
Ethylene promotes senescence of
leaves and petals
Air (control) 7 days ethylene

In gas-lit houses,
plants were harmed
Cotton plants by the ethylene
Ethylene promotes produced from
burning gas.
leaf and petal Aspidistra is
senescence. ethylene- resistant
and so became
popular houseplant.
Beyer, Jr., E.M. (1976) A potent inhibitor of ethylene action in plants. Plant Physiol. 58: 268-271.
 Ethylene shortens the longevity of
cut flowers and fruits
Ethylene levels can be
managed to maintain
fruit freshness,
commercially and at
home.

Strategies to limit ethylene effects

Limit production - high CO2 or low O2
Removal from the air -KMnO4 reaction, zeolite absorption
Interfere with ethylene binding to receptor - sodium thiosulfate
(STS), diazocyclopentadiene (DACP), others

Reprinted from Serek, M., Woltering, E.J., Sisler, E.C., Frello, S., and Sriskandarajah, S. (2006) Controlling ethylene
responses in flowers at the receptor level. Biotech. Adv. 24: 368-381 with permission from Elsevier.
 Molecular genetic approaches can
limit ethylene synthesis
ACC ACC
synthase oxidase
H H
C C
H H
S-adenosyl ACC Ethylene
methionine (1-aminocyclopropane-1-
carboxylic acid)

Antisense Introduction of antisense

ACC synthase
constructs to interfere with
expression of biosynthesis
enzymes is an effective way
Control to control ethylene production.

Theologis, A., Zarembinski, T.I., Oeller, P.W., Liang, X., and Abel, S. (1992) Modification
of fruit ripening by suppressing gene expression. Plant Phys. 100: 549-551.
 Ethylene-regulated gene
expression is negatively regulated
Air Ethylene
Air

In the absence of
Receptor ethylene, CTR binds
the receptor and
prevents transcription.
CTR
Ethylene binding to
the receptor releases
CTR, permitting
transcription.

Benavente, L.M., and Alonso, J.M. (2006) Molecular mechanisms of ethylene signaling in Arabidopsis. Mol. BioSyst. 2: 165–173. Reproduced by
permission of The Royal Society of Chemistry (RSC) for the European Society for Photobiology, the European Photochemistry Association, and the
RSC. Diagram adapted from Cuo, H., and Ecker, J.R. (2004) The ethylene signaling pathway: new insights. Curr. Opin. Plant Biol. 7: 40-49.
 Ethylene perception mutants
Air or interfere with ripening
Ethylene

Wild type

Receptor Several mutations Green-ripe

that affect ethylene
perception and
CTR signaling interfere Never-ripe2
with fruit ripening.

Never-ripe

Barry, C. S., et al. (2005) Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato. Plant Physiol. 138: 267-275.
Abscisic acid

•Seed maturation and

dormancy
•Desiccation tolerance
•Stress response
•Control of stomatal
aperture
ABA accumulates in maturing
seeds
Seed maturation
requires ABA
synthesis and
Embryonic
patterning
accumulation of
specific proteins
to confer
Reserve desiccation
accumulation tolerance to the
seed.

Desiccation
tolerance
ABA synthesis and signaling is
required for seed dormancy
Loss of function of
ABA ABA signaling
(protein kinase or
transcription factor
Protein
Kinase function) interferes
with ABA-induced
dormancy and
Transcription causes precocious
Factor
germination.

Transcription

Nakashima, K., et al. (2009) Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA
signaling are essential for the control of seed development and Dormancy. Plant Cell Physiol. 50: 1345–1363. Copyright (c) 2009 by the the Japanese
Society of Plant Physiologists with permission from Oxford University Press. McCarty, D.R., Carson, C.B., Stinard, P.S., and Robertson, D.S. (1989)
Molecular analysis of viviparous-1: An abscisic acid-insensitive mutant of maize. Plant Cell 1: 523-532.
Once dormant and dry, seeds can
remain viable for very long times
These date palm seeds are nearly 2000 Date palm growing
years old, but still viable and capable of from 2000 year old
germination. Five seed.
-hundred year old lotus seeds have also
been successfully germinated. Having a
thick seed coat may help these super
seeds retain viability.

From Sallon, S., et al. (2008). Germination, genetics, and growth of an ancient date seed. Science 320: 1464, with permission from AAAS Lotus picture by Peripitus
GA is required for seed
germination
Seed germination Reserve
requires elimination mobilization
of ABA and
production of GA to Cell
promote growth expansion
and breakdown of
seed storage
products.

GA
ABA
GA is used by brewers to promote
barley germination
Breakdown of starch in the
endosperm is initiated by GA
produced by the endosperm or
added during the malting process.

GA

GA amylase

sugars starch
Embryo

Endosperm Aleurone

Images by Prof. Dr. Otto Wilhelm Thomé Flora von Deutschland, Österreich und der Schweiz 1885 and Chrisdesign.
Summary – hormonal regulation
of reproductive development
GA and ethylene promote flowering in some
plants.

Fruit growth, maturation and ripening are

regulated by auxin, GA and ethylene.

Seed maturation and germination are

regulated by ABA and GA.

Understanding the roles of hormones in plant

reproduction is important for food production,
because most of our caloric intake is derived
from seeds.
 Hormonal responses to abiotic
stress
Air pollution
Photooxidative
stress

High Wounding and

Plants’ lives are
temperature mechanical
very stressful.....
stress damage

Water deficit, ABA and ethylene

drought help plants
Cold and respond to stress.
Soil salinity freezing
stress

Reprinted by permission from Macmillan Publishers, Ltd. Nature Chemical Biology. Vickers, C.E., Gershenzon, J., Lerdau, M.T., and Loreto, F.
(2009) A unified mechanism of action for volatile isoprenoids in plant abiotic stress Nature Chemical Biology 5: 283 - 291 Copyright 2009.
ABA biosynthesis is strongly
regulated

ABA levels are tightly controlled.

Critical steps in ABA biosynthesis
(circled in red) are encoded by
multiple tightly regulated genes to
ensure rapid and precise control.

Reprinted from Nambara, E., and Marion-Pol, A. (2003) ABA action and interactions in
seeds. Trends Plant Sci. 8: 213-217 with permission from Elsevier.
ABA synthesis is strongly induced
in response to stress

[ABA]
Leaf
µg/g dry
water
weight
potential
(atm)

Hours of drought stress

ABA levels rise during drought stress

due in part to increased biosynthesis
R.L. Croissant, , Bugwood.org www.forestryimages.org . Zabadel, T. J. (1974) A water potential
threshold for the increase of abscisic acid in leaves. Plant Physiol. (1974) 53: 125-127.
ABA induces stress-responsive
genes
Osmoprotectants Membrane and
(sugars, proline, protein stabilization
glycine betaine) (HSPs, LEAs)

H2O2
Movement of
Oxidative stress responses water and ions
– peroxidase, superoxide (aquaporins, ion
dismutase channels)
O2¯
ABA binding to an intracellular
receptor initiates transcriptional
responses

PYL1 is an ABA receptor.

When PYL1 binds ABA, it
also binds the protein
phosphatase PP2C,
inhibiting its function.

Reprinted by permission of Macmillan Publishers Ltd. Miyazono, K., et al. (2009) Structural basis of abscisic acid signalling. Nature 462: 609-614.
 ABA signal transduction affects
gene expression High ABA
Low ABA
ABA
PYL1 When ABA is present, PYL1
inactivation of the PP2C
phosphatase permits a protein
PP2C
PP2C
kinase (e.g. SnRK) to
phosphorylate and activate
ABA-inducible TFs, promoting P
SnRK transcription of ABA-inducible
TF
genes.
P

Transcription
ABA regulates stomatal aperture by
changing the volume of guard cels

Pairs of guard cells

surround the
openings of plant
pores called stomata.

Image buYizhou Wang, University of Glasgow

Guard cells control the opening and closing of stomata to

regulate gas exchange: a fine balance is required to allow
CO2 in for photosynthesis and prevent excessive water loss.

Guard cell image © John Adds, obtained through the SAPS Plant Science Image Database.
ABA controls stomatal aperture by
changing the volume of guard cels

When stomata are open, plants lose water through

transpiration. ABA induced by drought causes the guard cells to
close and prevents their reopening, conserving water.

Sirichandra, C., Wasilewska, A., Vlad, F., Valon, C., and Leung, J. (2009)The guard cell as a single-cell model towards understanding drought tolerance and abscisic
acid action. Journal of Experimental Botany 2009 60: 1439-1463. © The Author [2009]. Published by Oxford University Press on behalf of the Society for
Experimental Biology.
ABA-induced stomatal closure is
extremely rapid and involves changes
in ion channel activities
ABA triggers an increase in cytosolic calcium
(Ca2+), which activates anion channels (A-)
allowing Cl- to leave the cell. ABA activates
channels that move potassium out of the cell
(K+out) and inhibits channels that move
Cl- potassium into the cell (K+in). The net result is a
large movement of ions out of the cell.
A- channel

As ions leave the cell, so does water (by

K+in channel osmosis), causing the cells to lose volume and
close over the pore.
K+
H2O

Adapted from Kwak JM, Mäser P, Schroeder JI (2008) The clickable guard cell, version II: Interactive model of guard cell signal
transduction mechanisms and pathways. The Arabidopsis Book, ASPB. doi: 10.1199/tab.0114.
 Hormonal responses to biotic
stress

Bacteria, Jasmonates
fungi,
viruses – Herbivores –
Biotrophic insects, other
organisms animals, fungi –
Necrotrophic
organisms
Salicylic Acid

Photo credits: A. Collmer, Cornell University; Salzbrot.

Jasmonates

•Response to
necrotrophic pathogens
•Induction of anti-
herbivory responses
•Production of
herbivore-induced
volatiles to prime other
tissues and attract
predatory insects
JA biosynthesis
Cytoplasm
JAR1
conjugation

JA-ILE

JA conjugated to
isoleucine (JA-
ILE) is the active
compound.

From Acosta, I., et al. (2009) tasselseed1 is a lipoxygenase affecting jasmonic acid signaling in sex determination of
maize. Science 323: 262 – 265. Reprinted with permission from AAAS.
Jasmonate signaling contributes to
defense against herbivory
WT Mutant without JA

When exposed to hungry

fly larvae, plants unable
to produce JA have low
rates of survival.

McConn, M., et al. (1997) Jasmonate is essential for insect defense in Arabidopsis. Proc. Natl. Acad. Sci. USA 94: 5473-5477.
Jasmonates induce the expression
of anti-herbivory chemicals
Wound-induced signals
Insect oral secretions

Protease inhibitors
Feeding deterants

R.J. Reynolds Tobacco Company Slide Set and R.J. Reynolds Tobacco Company, Bugworld.org
Jasmonates contribute to
systemic defense responses

Defense
responses are
activated in
distant tissues
Jasomonates stimulate production of
volatile signaling compounds

Herbivore-induced volatiles prime

other tissues (and other plants) for
attack making them unpalatable
(indicated in red).
Reprinted from Matsui, K. (2006) Green leaf volatiles: hydroperoxide lyase pathway of
oxylipin metabolism. Curr. Opin. Plant Biol. 9: 274-280, with permission from Elsevier.
Herbivore-induced volatiles are
recognized by carnivorous and
parasitoid insects

Tim Haye, Universität Kiel, Germany Bugwood.org; R.J. Reynolds Tobacco Company Slide Set and R.J. Reynolds Tobacco Company, Bugworld.org
JA-induced changes in gene
expression
Low JA-Ile High JA-Ile
Defense
JAZ genes
JA- protein
responsive
transcription
factor

No JA-Ile
F-box protein transcription
receptor Transcription
(COI1)

Proteolysis
 Salicylic Acid – plant hormone and
painkiller
•Response to biotrophic
pathogens
•Induced defense
response
•Systemic acquired
resistance Salicylic Acid
Salicylic acid is named for
the willow Salix whose
analgesic properties were
known long before the
chemical was isolated.
Acetylsalicylic Acid - aspirin
Photo credit: Geaugagrrl
Salicylate synthesis is induced
upon pathogen attack
Control +Pathogen
Isochorismate
synthase (ICS) is
induced by pathogen
infection

SA accumulation induces PATHOGENESIS

RELATED (PR) and other defense genes
Reprinted from Métraux, J.P. (2002) Recent breakthroughs in the study of salicylic acid biosynthesis. Trends Plant Sci. 7: 332-334, with permission from Elsevier.
Reprinted by permission from Macmillan Publishers Ltd. Wildermuth, M.C., Dewdney, J., Wu, G., and Ausubel, F.M. (2001). Isochorismate synthase is required to
synthesize salicylic acid for plant defence. Nature 414: 562-565 copyright 2001.
Salicylates contribute to systemic
acquired resistance
SA is necessary in SAR
systemic tissue for SA
SAR, but the MeSA
nature of the
mobile signal(s) is
still up in the air
MeSA

It is likely that
multiple signals MeSA
contribute to SAR SA
Plants recognize PAMPS (pathogen-
associated molecular patterns)
PAMP-triggered
immunity
Flagellin is a conserved
bacterial protein recognized
by plants, also known as a
pathogen-associated
molecular pattern (PAMP).
SA
Recognition of PAMPs by a
plant cell triggers a set of
Immune immune responses that are
Responses
mediated by salicylic acid.

Redrawn from Pieterse, C.M.J, Leon-Reyes, A., Van der Ent, S., and Saskia C M Van Wees, S.C.M. (2009) Nat. Chem. Biol. 5: 308 – 316.
Some pathogens elicit a stronger
defense response
Effector-triggered
Many plants express resistance genes that immunity
recognize the effects of bacterial proteins
effector proteins.

The interaction of an R protein with an R

effector protein promotes a stronger immune
response, including the hypersensitive SA
response.

Immune
Responses

Redrawn from Pieterse, C.M.J, Leon-Reyes, A., Van der Ent, S., and Saskia C M Van Wees, S.C.M. (2009) Nat. Chem. Biol. 5: 308 – 316.
The hypersensitive response
involves cell death
Effector-triggered
immunity

SA
Pathogen Response (PR) genes
Antimicrobial compounds
Immune
Strengthening of plant cell walls Responses
Programmed cell death
Hypersensitive response (HR)

From Cawly, J., Cole, A.B., Király, L., Qiu, W., and Schoelz, J.E. (2005) The plant gene CCD1 selectively blocks cell death during the
hypersensitive response to cauliflower mosaic virus infection. MPMI 18: 212-219; Redrawn from Pieterse, C.M.J, Leon-Reyes, A., Van der Ent,
S., and Saskia C M Van Wees, S.C.M. (2009) Nat. Chem. Biol. 5: 308 – 316.
The hypersensitive response seals the
pathogen in a tomb of dead cells
HR

No HR

Without a hypersensitive
The HR kills the infected cells and response, the pathogen
cells surrounding them and prevents can multiply.
the pathogen from spreading.

Drawing credit Credit: Nicolle Rager Fuller, National Science Foundation; Photo reprinted by permission of Macmillan Publishers Ltd. Pruitt,
R.E., Bowman, J.L., and Grossniklaus, U. (2003) Plant genetics: a decade of integration. Nat. Genet. 33: 294 – 304.
Other hormones affect defense
response signaling
As part of their immune
responses, plants modulate
synthesis and response to
other hormones. Some
pathogens exploit the
connections between
growth hormones and
pathogen-response
hormones to their own
advantage, by producing
“phytohormones” or
interfering with hormone
signaling.

Reprinted from Robert-Seilaniantz, A., Navarro, L., Bari, R., and Jones, J.D.G. (2007). Pathological hormone imbalances. Curr. Opin. Plant Biol. 10: 372–379.
with permission from Elsevier.
 Summary – stress responses
Hormonal signaling is critical for plant
defenses against abiotic and biotic stresses.

ABA and ethylene are produced in stressed

plants and critical for activating their defense
pathways.

JA and SA contribute to local and systemic

defenses against pathogens.

Understanding plant hormonal responses to

stress is needed to improving agricultural
yields. Abiotic and biotic stresses are major
causes of crop losses and reduced yields
and which must be minimized.
 Crosstalk between hormone
signaling pathways

H1 H1 H2 H1 H2

Response Response

Crosstalk (or cross-regulation) occurs when two

pathways are not independent. It can be positive
and additive or synergistic, or negative.
 Crosstalk between hormone
signaling pathways

H1 H1 H2 H1 H2 H2 H1 H2

Response Response Response

Crosstalk can affect the

Crosstalk (or cross-regulation) occurs when two
synthesis, transport or
pathways are not independent. It can be positive
signaling pathway of another
and additive or synergistic, or negative.
hormone.
 Synergistic requirement for JA and
ET signaling in defense response
JA and ET signaling are
both required for high-
NO JA NO ET level expression of ERF1,
response response a TF that induces
defense gene expression
JA

and ERF1 ERF1

ET Defense
genes

Lorenzo, O., Piqueras, R., Sánchez-Serrano, J.J., and Solano, R. (2003) ETHYLENE RESPONSE FACTOR1
integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell 15: 165-178.
 Negative interaction between JA
and SA in defense responses
In defense signaling,
the JA and SA
pathways are mutually
antagonistic (locally),
and both are
antagonized by ABA.

Why does ABA reduce SA and JA

signaling? Perhaps a plant that is
already stressed and producing high
levels of ABA may be better off
temporarily restricting its responses to
pathogens.
Reprinted from Spoel, S.H., and Dong, X. (2008) Making sense of hormone crosstalk during plant immune responses. Cell Host Microbe 3:
348-351 with permission from Elsevier.
 GA and DELLAs interact extensively
with other signaling pathways

Just about every stage of the

plants life is coordinated by GA
and its effects on the other
hormone signaling pathways.

Although it is clear that GA’s effects

on DELLA proteins are very
important, we don’t yet understand
what these proteins do.

Weiss, D., and Ori, N. (2007) Mechanisms of cross talk between gibberellin
and other hormones. Plant Physiol. 144: 1240–1246.
GA and DELLAs interact extensively
with other signaling pathways
ABA
AUXIN

CK
ET ABA

Weiss, D., and Ori, N. (2007) Mechanisms of cross talk between gibberellin
and other hormones. Plant Physiol. 144: 1240–1246.
Ongoing research

• Hormones coordinate plant growth and defense

• Many aspects of hormone synthesis, homeostasis
and signaling are still being discovered
• Knowledge of these processes provides
tremendous opportunities for agricultural
improvements including the development of stress-
resistant and pathogen-resistant plants, plants with
greater abilities to take up nutrients, foods that stay
fresh longer, and increased crop yields
The field of plant hormones is
blossoming and fruitful