Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Scribd Logo
Upload

Welcome to Scribd!

  • 152 views

    Dentinogenesis: DR Saqlain Bin Syed Gilani

    Uploaded by

    Natasha Baloch
    Dentinogenesis begins when the tooth germ reaches the bell stage. Odontoblasts differentiate from dental papilla cells and secrete an organic matrix containing collagen fibers. Mineralization of the matrix occurs through matrix vesicles, forming mantle dentin. Secondary and tertiary dentin are deposited more slowly by the same odontoblasts or odontoblast-like cells in response to injury over time.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    Dentinogenesis: DR Saqlain Bin Syed Gilani

    Uploaded by

    Natasha Baloch
    152 views16 pages
    Dentinogenesis begins when the tooth germ reaches the bell stage. Odontoblasts differentiate from dental papilla cells and secrete an organic matrix containing collagen fibers. Mineralization of the matrix occurs through matrix vesicles, forming mantle dentin. Secondary and tertiary dentin are deposited more slowly by the same odontoblasts or odontoblast-like cells in response to injury over time.

    Original Title

    Dentinogenesis.pptx

    Copyright

    © © All Rights Reserved

    Available Formats

    PPTX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Dentinogenesis begins when the tooth germ reaches the bell stage. Odontoblasts differentiate from dental papilla cells and secrete an organic matrix containing collagen fibers. Mineralization of the matrix occurs through matrix vesicles, forming mantle dentin. Secondary and tertiary dentin are deposited more slowly by the same odontoblasts or odontoblast-like cells in response to injury over time.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    152 views16 pages

    Dentinogenesis: DR Saqlain Bin Syed Gilani

    Uploaded by

    Natasha Baloch
    Dentinogenesis begins when the tooth germ reaches the bell stage. Odontoblasts differentiate from dental papilla cells and secrete an organic matrix containing collagen fibers. Mineralization of the matrix occurs through matrix vesicles, forming mantle dentin. Secondary and tertiary dentin are deposited more slowly by the same odontoblasts or odontoblast-like cells in response to injury over time.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 16

    DENTINOGENESIS

    Dr Saqlain Bin Syed Gilani


    Di f
    the ferent
    Od iatio
    on t
    ob l n of
    asts

    De p
    org ositio
    ani
    c m n of th
    DENTINOGENESIS

    atri
    x e

    Min
    O. eraliz
    Ma
    trix ation
    of

    Se c
    Den ondar
    tin y
    fo rm
    atio
    n

    Ter
    formtiary D
    atio enti
    n n
     Dentin formation begins when the tooth germ reaches
    the bell stage
     Enamel organ is formed
     Internal enamel epithelium ready to secrete the matrix
     Dental papilla becomes the pulp
    DIFFERENTIATION OF ODONTOBLASTS
     Dentin forming cells arise from ectomesenchyme of
    dental papilla
     Neural crest in origin
     Internal Enamel Epithelium expresses signaling
    molecules and growth factors
     Influences the dental papilla
    • Small & undifferentiated
    • Nucleus lying in the center
    Dental Papilla

    • Separated from Dental Papilla by a layer an acellular layer


    • After the reversal of polarity changes occur in D.P
    IEE

    • Elongates, forms preodontoblasts  odontoblasts


    • Acellular zone gradually disappears with the increase in size of
    odontobalsts
    Ectomesen- • Newly formed cells are polarized with nucleus placed away from IEE
    -chymal cells
    A B C D
    MANTLE DENTIN
    Post cell differentiation, organic matrix has be to be formed

    First sign of dentin formation is the appearance of large collagen fibrils


    (0.1 – 0.2mm) (Von Korff’s fibers). Type III with fibronectin

    These fibers originate deep among odontoblasts, extend towards IEE and
    fan out in the structurless ground substance

    As Odontoblasts continue to increase in size, they produce Type I


    collagen fibers
    • Arranged parallel to the future EDJ

    Mantle Predentin is formed


     Coincident with collagen deposition, plasma membrane
    of odontoblasts adjacent to ameloblasts extend their
    processes into the extra cellular matrix
     Enamel Spindles
    • As odontoblasts form these processes
    • They bud off small vesicles near basal lamina
    Matrix
    Vesicles

    • Cell process (odontoblast process) is formed


    • O.P left behind in the matrix as odontoblast moves pulpally
    Odontoblast
    cell Process

    • Mineral phase first appears in the matrix vesicles as single crystals


    (seeded by phosphplipids)
    • These crystals grow in size and rupture out as clusters, fusing with the
    Mineral adjacent clusters and form a continuous layer of mineralized matrix
    Phase
     Deposition of mineral lags behind the formation of
    organic matrix
     Non collagenous proteins regulate mineralization

    (A)Predentin & A
    (B) Odontoblast
    process
    B
    VASCULAR SUPPLY DURING
    MINERALIZATION

    Subodontoblastic Layer
    Mantle dentin

    Migrate B/W
    Odontoblasts Circumpulpal
    & dentin
    Endothelial lining fenestrate

    Retreat & lining becomes Completion of


    Dentinogenesis
    Continuous
    CONTROL OF MINERALIZATION
     Odontoblasts control initiating mineralization by matrix
    vesicles & regulating proteins
     Calcium L type have been demonstrated in the basal
    lamina
     Blocking these Ca channels can affect mineralization
     Presence of alkaline phosphatase and calcium
    adenosinetriphosphatase activity in the distal end aids in
    transport of mineral ions
    PATTERN OF MINERALIZATION

    Pattern
    Globular
    Linear
    ROOT DENTINOGENESIS
     Hertwig root sheath epithelial cells initiate odontoblast
    differentiation
     Some differences from coronal dentinogenesis

     Outermost layer (similar to mantle dentin in crown) has


    different collagen fiber orientation
     Phosphoprotein content may also differ
    SECONDARY & TERTIARY
    DENTINOGENESIS
     Secondary;
     Post root formation, by the same odontoblasts
     Continuation of primary dentin but much slower in pace
     May have crowding of odontoblast cells or absence (in case
    of death)
     Tertiary;
     Remaining odontoblasts or odontoblast like cells
     Rate of deposition depends on the rate of injury
     In case of rapid deposition
     Irregular tubular structure maybe formed
    REFERENCES
     Berkovitz, pages: 329 – 338
     Ten Cate’s 8th Edition, pages: 170 - 204

    You might also like