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Leonardo Brauna
  • +5561992714178
Research Interests:
Desenvolveu metodologias in vitro procurando avaliar a inibição da germinação miceliogênica dos escleródios de Sclerotinia sclerotiorum, agente causal do mofo branco nas culturas de soja, feijão, entre outras, por isolados de Trichoderma
Research Interests:
From 40 soil samples collected in the Rio Grande do Sul, Minas Gerais, São Paulo, Bahia, Goiás and Tocantins states, Brazil, a collection of 230 monosporic isolates of Trichoderma spp. was established using TSM selective media. In order... more
From 40 soil samples collected in the Rio Grande do Sul, Minas Gerais, São Paulo, Bahia, Goiás and Tocantins states, Brazil, a collection of 230 monosporic isolates of Trichoderma spp. was established using TSM selective media. In order to select efficient isolates for biological control, dual culture tests were carried out on PDA media at 20 ºC for Sclerotinia sclerotiorum and at 25 ºC for Fusarium solani f.sp. phaseoli. From the whole collection, 50 isolates presented antagonism against F. solani and 111 isolates to S. sclerotiorum. The antagonism against both pathogens was found in only 10% of the isolates. Scanning electron microscopy assessments with seven in vitro selected isolates showed that not all promoted hyperparasitism on the pathogens, suggesting the existence of other mechanisms of antagonism, as antibiosis or competition.
The interaction between Dicyma pulvinata and Fusicladium macrosporum was studied by scanning electron microscopy. Spores of D. pulvinata germinated on the surface of F. macrosporum lesions induced on artificially infected rubber plants... more
The interaction between Dicyma pulvinata and Fusicladium macrosporum was studied by scanning electron microscopy. Spores of D. pulvinata germinated on the surface of F. macrosporum lesions induced on artificially infected rubber plants were fixed 8 h after inoculation. D. pulvinata germ tubes seemed to elongate toward F. macrosporum. Close contact between the antagonistic fungus and F. macrosporum spores was verified 24 h after application of D. pulvinata. At the end of the process, spores of F. macrosporum seemed to have disintegrated and to be devoid of content. The hyperparasite grew completely over the pathogen. Six to seven days after application of the antagonistic fungus, D. pulvinata conidiophores were observed emerging from F. macrosporum structures with profuse sporulation. Studies have also shown the possibility of D. pulvinata producing hydrolytic enzymes, which could be associated with the control of plant pathogens. This information may help to elucidate some of the modes of action of D. pulvinata, a potential biological control agent for South American leaf blight of Hevea rubber plant.
Research Interests:
Apresentação realizada no Ministério da Agricultura sobre a reavaliação de herbicidas de uso na soja.
Research Interests: