Pinar Ciragil
Çukurova University, Pharmacy, Faculty Member
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Background In this study, we aimed at determining the incidence of bacteremia during septoplasty and open septorhinoplasty. Methods The study included 60 patients (30 septoplasties and 30 open septorhinoplasties). Preoperative nasal... more
Background In this study, we aimed at determining the incidence of bacteremia during septoplasty and open septorhinoplasty. Methods The study included 60 patients (30 septoplasties and 30 open septorhinoplasties). Preoperative nasal cultures from the nasal cavity and vestibule were taken by using swabs, and blood cultures were obtained from peripheral veins preoperatively, intraoperatively, and postoperatively. Blood cultures were evaluated by using the BACTEC method. Results Neither the blood cultures taken preoperatively nor those obtained postoperatively was positive for any organisms. On the other hand, although the bacterial growth was observed in only one of the blood cultures (3.3%) taken intraoperatively during septoplasty, it was observed in four blood cultures (13.3%) obtained intraoperatively during open septorhinoplasty. Conclusion Our data indicate that a transient bacteremia occurs during open septorhinoplasty. Although this bacteremia is transient and it has not led t...
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Recent progress in the understanding of psoriasis has shown that the regulation of local and systemic cytokines plays an important role in its pathogenesis. The most often used psoriasis score is the psoriasis area and severity index... more
Recent progress in the understanding of psoriasis has shown that the regulation of local and systemic cytokines plays an important role in its pathogenesis. The most often used psoriasis score is the psoriasis area and severity index (PASI). A simple laboratory test from a blood sample would be an attractive, patient-independent, and observer-independent marker of disease severity. To this end, we evaluated the association of serum levels of some proinflammatory cytokines in vivo and their correlation with severity of psoriasis. The serum levels of cytokines levels were determined with the use of the ELISA method. All mean values except IL-17 levels of patients were significantly higher than those of controls. There was a significant correlation between serum levels of IFN-γ, IL-12, IL-17, and IL-18, and severity of the disease. Psoriasis can be described as a T-cell-mediated disease, with a complex role for a variety of cytokines, which has led to the development of new immunomodul...
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The purpose of this study was to determine the effect of urinary tract infection (UTI) on antioxidant systems and lipid peroxidation (LPO) levels during pregnancy. We also investigated if these antioxidant systems and LPO levels differed... more
The purpose of this study was to determine the effect of urinary tract infection (UTI) on antioxidant systems and lipid peroxidation (LPO) levels during pregnancy. We also investigated if these antioxidant systems and LPO levels differed in each trimester. One hundred forty-three nonpregnant women, as a control group, and 77 pregnant women were included in the study. Urine cultures were performed according to standard techniques. Catalase (CAT), superoxide dismutase (SOD), and LPO levels were measured using a spectrophotometer. UTI was observed in 14 of 77 pregnant women and the isolated microorganisms were Escherichia coli, Klebsiella pneumoniae, andStaphylococcus saprophyticus. CAT, SOD, and LPO levels were increased in pregnant women compared with nonpregnant women (P <.01). CAT, SOD activities, and LPO levels were increased from the first trimester to the third trimester in pregnancy without UTI. However, CAT and SOD activities were decreased, LPO levels were increased from t...
The purpose of this study was to determine the effect of urinary tract infection (UTI) on antioxidant systems and lipid peroxidation (LPO) levels during pregnancy. We also investigated if these antioxidant systems and LPO levels differed... more
The purpose of this study was to determine the effect of urinary tract infection (UTI) on antioxidant systems and lipid peroxidation (LPO) levels during pregnancy. We also investigated if these antioxidant systems and LPO levels differed in each trimester. One hundred forty-three nonpregnant women, as a control group, and 77 pregnant women were included in the study. Urine cultures were performed according to standard techniques. Catalase (CAT), superoxide dismutase (SOD), and LPO levels were measured using a spectrophotometer. UTI was observed in 14 of 77 pregnant women and the isolated microorganisms were Escherichia coli, Klebsiella pneumoniae, and Staphylococcus saprophyticus. CAT, SOD, and LPO levels were increased in pregnant women compared with nonpregnant women (P < .01). CAT, SOD activities, and LPO levels were increased from the first trimester to the third trimester in pregnancy without UTI. However, CAT and SOD activities were decreased, LPO levels were increased from...
Recent progress in the understanding of psoriasis has shown that the regulation of local and systemic cytokines plays an important role in its pathogenesis. The most often used psoriasis score is the psoriasis area and severity index... more
Recent progress in the understanding of psoriasis has shown that the regulation of local and systemic cytokines plays an important role in its pathogenesis. The most often used psoriasis score is the psoriasis area and severity index (PASI). A simple laboratory test from a blood sample would be an attractive, patient-independent, and observer-independent marker of disease severity. To this end, we evaluated the association of serum levels of some proinflammatory cytokines in vivo and their correlation with severity of psoriasis. The serum levels of cytokines levels were determined with the use of the ELISA method. All mean values except IL-17 levels of patients were significantly higher than those of controls. There was a significant correlation between serum levels of IFN-γ, IL-12, IL-17, and IL-18, and severity of the disease. Psoriasis can be described as a T-cell-mediated disease, with a complex role for a variety of cytokines, which has led to the development of new immunomodul...
The analytical, intra-individual and inter-individual variations were determined for xanthine oxidase (XO) and myeloperoxidase (MPO) and the reference values were established. A total of 380 apparently healthy people, 177 male and 203... more
The analytical, intra-individual and inter-individual variations were determined for xanthine oxidase (XO) and myeloperoxidase (MPO) and the reference values were established. A total of 380 apparently healthy people, 177 male and 203 female (from 20 to 82 years, average 40 years old), were randomly selected from villages and cities of the southern part of Turkey. XO and MPO activities in human sterile urine samples were measured by spectrophometric method. The distributions were Gaussian and no significant difference was observed between the male and female subjects. The mean value (standard deviation) of the population investigated for XO was 414.0 (93.3) U/L, and for MPO 104.5 (49.2) U/L, respectively. The analytical, intra-individual and interindividual variations were assessed in 20 apparently healthy subjects and were found to be; XO: 6.2 %, 21.2%, and 19.2% MPO: 4.0%, 16.4%, 11.7% respectively. The results of the index individuality showed that reference values of XO and MPO ...
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Otitis media with effusion (OME), a common disease in childhood, is characterized by fluid accumulation in the middle ear behind an intact tympanic membrane without signs and symptoms of acute infection [1]. OME has been considered as a... more
Otitis media with effusion (OME), a common disease in childhood, is characterized by fluid accumulation in the middle ear behind an intact tympanic membrane without signs and symptoms of acute infection [1]. OME has been considered as a multifactorial disease ...
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Turkce Ozet: Amac: Bruselloz, dunyanin bircok ulkesinde ve Turkiye\'de yaygin olarak gorulen, ciddi ekonomik kayiplara neden olan zoonotik bir hastaliktir. Bu calismanin amaci Kahramanmaras il merkezinde 15 yas ve uzeri nufusta Rose... more
Turkce Ozet: Amac: Bruselloz, dunyanin bircok ulkesinde ve Turkiye\'de yaygin olarak gorulen, ciddi ekonomik kayiplara neden olan zoonotik bir hastaliktir. Bu calismanin amaci Kahramanmaras il merkezinde 15 yas ve uzeri nufusta Rose Bengal testi (RBT) ve Standart Tup Aglutinasyon (STA) testi kullanilarak bruselloz seroprevalansini saptamak ve bruselloz ile ilgili bazi sosyodemografik faktorleri degerlendirmektir. Yontem: Calisma, Haziran 2006-Haziran 2007 tarihleri arasinda kent merkezinde yasayan kisilerden kume orneklemesi yontemiyle secilen 15 yas ve uzeri 1100 kiside gerceklestirildi. Arastirmaya alinan kisilerin sosyodemografik ozelliklerini, meslek gruplarini, hayvan besleme durumlarini, sut ve sut urunleri tuketim bicimlerini ve bruselloza bagli semptomlarini saptamak amaciyla bir anket uygulandi. Arastirmaya alinan 1100 kisinin serum ornekleri RBT ve STA testi ile incelendi. Bulgular: Degerlendirilen kisilerin 1089\'unun (%99) her iki test yontemi ile seronegatif, 11\'inin (%1) ise seropozitif oldugu saptandi. Cinsiyet, yas, egitim durumu, meslek gruplari, hayvancilik ugrasi, hayvanlarin veteriner kontrolunde ve asili olma durumlari ve dondurma tuketim durumlari ise seropozitiflikle iliskili bulunmadi (p>0.05). Sutu kaynatarak tuketenlerle, kaynatmadan tuketenler arasinda seropozitiflik acisindan istatistiksel olarak anlamli bir fark gozlendi (p<0.001). Kaynatilmis peynir tuketenlerle, taze peynir tuketenler arasinda brusella seropozitifligi acisindan anlamli bir fark gozlendi (p<0.001). Brusellozu dusunduren yakinma oykusu ile brusella seropozitifligi arasindaki iliski istatistiksel olarak anlamli bulundu (p<0.001). Sonuc: Calismamiza dayanarak, yoremizde brusella seroprevalansinin yoresel sut ve peynir tuketim sekli ile iliskili oldugu soylenebilir. Bu acidan halka brusella hastaligi ve bulasma yollari ile ilgili egitim verilmesi hastaligin onlenmesinde onemli olabilir. Ureticiler de brusellozla ilgili bilgilendirilmelidir. Tarim ve veteriner teskilati ile isbirligi saglanmasi ve saha calismalarina onem verilmesi hastaligin kontrolunde onemlidir.
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Step 3 A thin rubber or a plastic tube is passed into the nose and brought out through the oral cavity. The two ends are pulled and fixed at the angle of the mouth (Figure 3), thus pulling or stiffening or tightening the soft palate. The... more
Step 3 A thin rubber or a plastic tube is passed into the nose and brought out through the oral cavity. The two ends are pulled and fixed at the angle of the mouth (Figure 3), thus pulling or stiffening or tightening the soft palate. The patient is then allowed to sleep. The controlled snore recording is repeated. A decrease or disappearance of snoring indicates that soft palate surgery alone will be beneficial for snoring. RR has used this method on five patients during the last 3 years to identify the level of obstruction before planning the surgery. The disadvantages of this method are the amount of time involved and possible discomfort
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This study was designed to compare the effect of pretreatment with N-acetylcysteine (NAC) and beta -glucan (beta GLU) on inflammatory response in a rat model of sepsis. The study was performed in the animal laboratory of the Kahramanmaras... more
This study was designed to compare the effect of pretreatment with N-acetylcysteine (NAC) and beta -glucan (beta GLU) on inflammatory response in a rat model of sepsis. The study was performed in the animal laboratory of the Kahramanmaras Sutcu Imam University, School of Medicine. Forty rats were randomized into four groups (control, sham, NAC, and beta GLU). Control and Sham groups received saline or NAC (200 mg/kg, po) in the NAC group and beta GLU (50 mg/kg, po) in the betaGLU group via intragastric gavage once a day for 10 days and 30 min prior to surgery. Sepsis was induced by cecal ligation and puncture (CLP) in rats. In the NAC, beta GLU, and control groups, a laparotomy was performed with the CLP procedure. In the sham group, laparotomy was performed and cecum was manipulated but not ligated or perforated. TNF-alpha and IL-6 levels were significantly elevated in the control group and decreased in the NAC and beta GLU groups. IL-10 levels were significantly increased in the beta GLU group (p &amp;amp;lt; .05). Superoxide dismutase and catalase levels in the liver tissue were significantly increased in the NAC and beta GLU groups, whereas superoxide dismutase levels were higher in the beta GLU pretreatment group than the NAC pretreatment group (p &amp;amp;lt; 0.05). Malondialdehyde levels in the liver tissue were significantly elevated in the control group and decreased in the NAC and beta GLU groups (p &amp;amp;lt; .05). Prophylactic administration of NAC or beta GLU similarly ameliorated sepsis syndrome by reduction of the proinflammatory cytokines and increase of the anti-inflammatory cytokine levels and accession of cellular antioxidants, which protect cells from oxidative stress, thereby recruiting inflammatory cells into tissue.
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... Auteur(s) / Author(s). EMEKDAS Gural (1) ; ASLAN Gonul (1) ; TEZCAN Seda (1) ; CIRAGIL Pinar (2) ; BAYRAKTAR Mehmet R. (3) ; ONLEN Yusuf (4) ; AKTAS Esin (5) ; BOSNAK Vusiat (6) ; KANIK Arzu (7) ; Affiliation(s) du ou des auteurs /... more
... Auteur(s) / Author(s). EMEKDAS Gural (1) ; ASLAN Gonul (1) ; TEZCAN Seda (1) ; CIRAGIL Pinar (2) ; BAYRAKTAR Mehmet R. (3) ; ONLEN Yusuf (4) ; AKTAS Esin (5) ; BOSNAK Vusiat (6) ; KANIK Arzu (7) ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s). ...
Research Interests: Tropical Medicine, Turkey, Medicine, Humans, Female, and 7 moreAnimals, Brucella, Male, Bacteria, Cattle, Brucellosis, and Medical and Health Sciences
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The majority of cystic fibrosis (CF) patients suffer from chronic respiratory infection with the opportunistic bacterial pathogen Pseudomonas aeruginosa. The virulence of P. aeruginosa is associated with the presence of various... more
The majority of cystic fibrosis (CF) patients suffer from chronic respiratory infection with the opportunistic bacterial pathogen Pseudomonas aeruginosa. The virulence of P. aeruginosa is associated with the presence of various extracellular factors, like alginate, elastase, alkaline protease which contribute tissue destruction and assist bacterial invasion. Virulence factor production of P. aeruginosa strains isolated from 46 CF patients followed in two cities in Turkey was detected. Strains were compared genotypically by arbitrarily primed PCR. Antimicrobial susceptibilities to 12 antibiotics were determined by broth microdilution method. Evaluation of virulence factor results revealed that 95.8% of the strains were alginate, 71.7% elastase and 52.1% alkaline protease producers. AP-PCR analysis revealed 35 genotypes indicated almost a complete discrepancy among the strains. The most effective drugs were penems and quinolones. Among aminoglycosides amikacin was the most effective o...
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The aim of this study was to evaluate if xanthine oxidase and myeloperoxidase levels quantitation method may alternate routine culture method, which takes more time in the diagnosis of urinary tract infections. Five hundred and forty-nine... more
The aim of this study was to evaluate if xanthine oxidase and myeloperoxidase levels quantitation method may alternate routine culture method, which takes more time in the diagnosis of urinary tract infections. Five hundred and forty-nine outpatients who had admitted to Clinic Microbiology Laboratory were included in the study. The microorganisms were identified by using VITEK System. The urine specimens that were negative from the quantitative urine culture were used as controls. The activities of MPO and XO in spot urine were measured by spectrophotometric method. Through the urine cultures, 167 bacteria were isolated from 163 urine specimens; 386 cultures yielded no bacterial growth. E. coli was the most frequent pathogen. In infection with E. coli both XO and MPO levels were increased the most. The sensitivity, specificity, positive predictive value, and negative predictive value for XO were 100%, 100%, 100%, and 100%, respectively. These values for MPO were 87%, 100%, 100%, and 94%, respectively. These data obtained suggest that urine XO and MPO levels may be new markers in the early detection of UTI.
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Research Interests: Biological Sciences, Humans, Escherichia coli, Candida, Candidiasis, and 19 moreBacteria, Staphylococcus aureus, Urinary Tract Infections, Pseudomonas aeruginosa, European, Anti-Bacterial Agents, Urinary Tract, Cost Analysis, Methicillin Resistance, Cost Saving, Antifungal Agents, Microbial Sensitivity Tests, Bacterial infections, Antimicrobial susceptibility testing, Pseudomonas Aeruginosa, Streptococcus agalactiae, Sensitivity and Specificity, Culture Media, and Mixed Culture
To investigate the impacts of interferon alpha-2b (IFN alpha-2b) on the oxidative stress states in the treatment of chronic hepatitis B (CHB) with different genotypes. Thirty-five patients with chronic hepatitis B and 18 healthy... more
To investigate the impacts of interferon alpha-2b (IFN alpha-2b) on the oxidative stress states in the treatment of chronic hepatitis B (CHB) with different genotypes. Thirty-five patients with chronic hepatitis B and 18 healthy volunteers as a control were enrolled in this present study. In control and patients group, the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), serum malondialdehyde (MDA) levels, serum total antioxidative stress capacity (TAC) were measured spectrophotometrically. After the therapy with interferon alpha-2b at the dose of 300 million units via intramuscular injection thrice a week for 12 weeks, these parameters were measured again in the patient group. The genotypes of hepatitis B virus were detected by polymerase chain reaction and hybridization. The effective group was defined as the patients with complete response and partial response. The elevated concentrations of MDA and impaired levels of TAC in the patients with CHB were observed as compared to the healthy controls (P &amp;amp;amp;amp;amp;amp;lt; 0.05 for both). There were no significant differences in serum levels of MDA and TAC in CHB patients with various genotypes (P &amp;amp;amp;amp;amp;amp;gt; 0.05). The serum levels of MDA after the treatment with IFN alpha-2b were significantly lower than the pretreatment levels (P &amp;amp;amp;amp;amp;amp;lt; 0.05), which even returned to the normal concentration (P &amp;amp;amp;amp;amp;amp;gt; 0.05) in the effective group. There were significant increases in the TAC after the IFN alpha-2b therapy in the effective group. However, the significant differences in the TAC levels before and after the INFalpha-2b treatment were not observed in the non-responsive group. The oxidative stress could be improved with IFN alpha-2b treatment of chronic hepatitis B patients. The results suggest that antioxidant treatment for chronic hepatitis B patients may help improve the effect of anti-virus therapy.
Research Interests: Treatment Outcome, Oxidative Stress, Adolescent, Antioxidants, Humans, and 16 moreFemale, Male, Young Adult, Spectrophotometry, Middle Aged, Genotype, Adult, Time Factors, Hepatitis B virus, Recombinant Proteins, chronic hepatitis B, Aspartate Aminotransferases, Malondialdehyde, Antiviral Agents, Interferon Alpha, and Alanine Transaminase
In this study, alginate, elastase and alkaline protease production of Pseudomonas aeruginosa, which are accepted as virulence factors, have been investigated in the strains isolated from 60 lower respiratory tract samples of cystic... more
In this study, alginate, elastase and alkaline protease production of Pseudomonas aeruginosa, which are accepted as virulence factors, have been investigated in the strains isolated from 60 lower respiratory tract samples of cystic fibrosis patients, together with the strains isolated from 59 lower respiratory tract, 62 urine and 32 blood samples of non-cystic fibrosis patients, in order to evaluate the relationship between the virulence factors and infected body sites. Our results indicated that all isolates including the ones from cystic fibrosis patients produced similar levels of alginate ranging between 1.830+/-1.666 and 1.305+/-1.690 microg/ml with the exception of urinary isolates for which the alginate amount was 0.9655+/-1.386 (p<0.05). Elastase levels exhibited no statistically significant difference among groups of isolates; and finally alkaline protease levels, although not statistically significant, was greater in urine isolates than blood and respiratory isolates; h...
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Although adenoidectomy is one of the most commonly performed surgical procedures in children, there is no satisfactory information about the risk of bacteremia during adenoidectomy and necessity of antibiotic use. The aim of this study... more
Although adenoidectomy is one of the most commonly performed surgical procedures in children, there is no satisfactory information about the risk of bacteremia during adenoidectomy and necessity of antibiotic use. The aim of this study was to determine the incidence of bacteremia during adenoidectomy and identify the organisms leading to bacteremia. Thirty two patients who had undergone adenoidectomy at ENT Clinic of Sutcu Imam University were included in the study. They had received no antimicrobial therapy for at least 20 days before surgery. Adenoidal surface and deep tissue cultures were taken and venous blood samples were obtained for cultures before and immediately after adenoidectomy in which adenoid was removed with a curette. While none of the blood cultures taken preoperatively was positive for any organisms, the cultures obtained postoperatively were positive in only two of 32 patients included in the study. The results of this study suggest that there is an extremely low incidence of bacteremia during adenoidectomy. As a result, it may be concluded that the use of prophylactic antibiotics to prevent bacteremia or its complications is unnecessary unless the patient has a predisposing factor for cardiac infection like prosthetic valve replacement.
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Research Interests: Immunology, Humans, Smoking, Female, Smokeless tobacco, and 7 moreMale, Aged, Middle Aged, Adult, Mediators, Immunoglobulins, and Humoral Immunity
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To investigate the effects of intraperitoneally administered lornoxicam on adhesion formation, bursting pressure, tissue antioxidant levels, morbidity and mortality after ileocolic anastomosis in a rat bacterial peritonitis model.... more
To investigate the effects of intraperitoneally administered lornoxicam on adhesion formation, bursting pressure, tissue antioxidant levels, morbidity and mortality after ileocolic anastomosis in a rat bacterial peritonitis model. Thirty-six rats were divided into three random groups. Bacterial peritonitis was induced by performing a cecal ligation and puncture, then the cecal was resected and ileocolic anastomosis was performed. Rats of groups 1, 2 and 3 were given 2 mL normal saline, 2 mL lornoxicam, and nothing, respectively. All groups were killed at day 14. Adhesions were scored, and the presence of intra-abdominal abscesses and fistulas were noted. Anastomotic healing was assessed by bursting pressure. Tissue antioxidant levels were tested from left abdominal walls. One day after cecal ligation and puncture, microbiological examination showed polymicrobial bacterial peritonitis. The rats treated with lornoxicam had significantly lower adhesion scores than did the saline and nothing treated rats (P = 0.007). Bursting pressures of groups were unaffected by the treatment. Tissue antioxidant levels of groups were affected by the treatment. Morbidity and mortality were similar in all groups. The present study demonstrated that a single intraperitoneal instillation of lornoxicam in buffer solution at the end of the surgery reduces adhesion formation in rats bacterial peritonitis model. It was also determined that lornoxicam had no negative effect on the healing of intestinal anastomosis, abscess and anastomotic leakage. Use of lornoxicam in peritonitis was effective in decreasing the oxidative stress of tissue during peritonitis.
Research Interests: Wound Healing, Oxidative Stress, Adhesion, Female, Animals, and 7 moreClinical Sciences, Pressure, Rats, Rat, Wistar Rats, Peritonitis, and PIROXICAM
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Comamonas testosteroni is an uncommon isolate in the clinical laboratory as a human pathogen. C. testosteroni most commonly emerges in abdominal pathologies especially in perforated appendicitis. In Turkey we report first time a case of... more
Comamonas testosteroni is an uncommon isolate in the clinical laboratory as a human pathogen. C. testosteroni most commonly emerges in abdominal pathologies especially in perforated appendicitis. In Turkey we report first time a case of bacteremia due to this organism, in a 22-year-old man with perforated acute appendicitis. The organism was shown to be susceptible to routine antibiotics so it was easily eliminated even after having caused a bacteremia.