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    Afshin Ghelichi

    The present paper aimed at evaluating nutritional value and fatty acid profile of muscle textures of the Caspian Sea wild carp, farmed wild carp and farmed carp. No significant differences were found between chemical composition indices... more
    The present paper aimed at evaluating nutritional value and fatty acid profile of muscle textures of the Caspian Sea wild carp, farmed wild carp and farmed carp. No significant differences were found between chemical composition indices (total lipid, crude protein, moisture) (p>0.05). However, ash contant was significantly higher in wild carp compared with farmed wild carp. There were not significantly differences of SFA, MUFA, PUFA, ω 3, ω 6, EPA+DHA, ω-3/ω-6 and PUFA/SFA between farmed wild carp and farmed carp. But following determination of fatty acid profiles in the Caspian Sea wild carp and farmed wild carp, fatty acid compositions in three groups of fish were found to be significantly different in terms of SFA, MUFA, PUFA, ω 3, EPA+DHA, ω-3/ω6 and PUFA/SFA. PUFA, ω 3, ω 6, EPA+DHA, ω-3/ω-6 and PUFA/SFA were significantly higher in the Caspian Sea wild carp. Considering exceeding PUFA/SFA and ω-3/ω-6 ratios in Caspian Sea wild carp, farmed wild carp and farmed carp, all fis...
    ... Afshin Ghelichi 1 , Nourmohammad Makhdoomi 2 , Sarah Jorjani 1 , Ali Taheri 2. 1 Department of fisheries, Islamic Azad University-Azadshahr Branch, Azadshahr, Iran 2 Shahid Marjani Sturgeon Culture Center, Golestan province, Iran. ...
    Fatty acids have several effects on immune responses. The present study was performed in order to determine the effect of replacement of fish oil (FO) by canola oil (CO) on blood serum enzymes, i.e. Aspartate aminotransferase (AST),... more
    Fatty acids have several effects on immune responses. The present study was performed in order to determine the effect of replacement of fish oil (FO) by canola oil (CO) on blood serum enzymes, i.e. Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Lactate dehydrogenase (LDH), and Alkaline phosphatase (ALP), of the rainbow trout (Oncorhynchusmykiss).Four experimental diets were formulated with partial or complete (0, 33%, 67%, and 100%) replacement of FOby CO.Fish were fed at 4% of their body weight three times a day (at 8, 12, and 18) for 50 days.Average temperature, dissolved oxygen, and pH were 14°C, 7.5 mg.lit, and 7.32, respectively. Measurement of ALT, AST, LDH, and ALP was performed by biochemistry analyzer (Eppendorf, Germany) and laboratory kits. Statistical analysis was performed by one-way ANOVA and Duncan’s multiple-range test (p<0.05).The results derived from the present study revealed that the fish fed by 100% fish oil had the highest level of AST, A...
    With considering the abundant sources of Kilka fish in Caspian Sea, the aim of this study was to produce a new product from Kilka in industrial scale with two different types of batters (simple and tempura batter) in order to increase... more
    With considering the abundant sources of Kilka fish in Caspian Sea, the aim of this study was to produce a new product from Kilka in industrial scale with two different types of batters (simple and tempura batter) in order to increase human consumption and evaluation of chemical, microbial and sensory properties.The Kilka fish were battered in the  simple and tempura batter, then the fish were breaded with conventional breading crumbs and then pre-fried at 170°C for 30 sec in sunflower oil .The fried samples were immediately frozen through continuous method at -40°C in a spiral freezer and they were packaged. The samples were kept  at -18°C. Chemical, microbial and sensory properties were performed on the fried breaded Kilka fish a day after freezing.  There were no significant difference found  in protein, fat and ash contents of raw breaded Kilka prepared from tempura and simple batters (P≥0.05) But there was significant difference in moisture content of raw breaded Kilka  prepare...
    This study aimed to investigate the effects of dietary olive leaf extract (OLE) on intestinal immune-related genes expression of tumor necrosis factor alpha (TNFa), interleukin 1 beta (IL1b), lysozyme (LYS), and mucin2 (MUC2). For this... more
    This study aimed to investigate the effects of dietary olive leaf extract (OLE) on intestinal immune-related genes expression of tumor necrosis factor alpha (TNFa), interleukin 1 beta (IL1b), lysozyme (LYS), and mucin2 (MUC2). For this purpose, common carp (~15 g) were fed with 0 (control), 0.1 (OLE-0.1), 0.5 (OLE-0.5) and 1 (OLE-1) % OLE diets for eight weeks. The fish were sampled after one and eight weeks to study intestinal TNFa, IL1b, LYS, and MUC2 gene expressions. The results showed that dietary OLE administration significantly up-regulated intestinal TNFa gene expression after one (all OLE-treated groups) and eight (OLE-0.5) weeks. Moreover, OLE-0.1 and OLE-1 groups showed up-regulated intestinal IL1b expression, after one week, all the OLE-treated fish had significantly higher intestinal IL1b expression, after eight weeks. OLE had no significant effects on LYS gene expression after one week, but OLE-0.1 and OLE-0.5 had significantly higher gene expressions after eight weeks...
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    An 8-week growth trial was carried out in freshwater fiberglass tanks to investigate the effects of different dietary prebiotic inulin levels on hematologic and biochemical parameters and some blood serum enzymes in juvenile great... more
    An 8-week growth trial was carried out in freshwater fiberglass tanks to investigate the effects of different dietary prebiotic inulin levels on hematologic and biochemical parameters and some blood serum enzymes in juvenile great sturgeon (Huso huso). Four experimental diets were formulated to contain different prebiotic inulin levels (0.0%, 1.0%, 2.0%, and 3.0%, respectively). Triplicate groups of fish (50 fish per tank with an average body weight of 16.14 ± 0.38 g) were assigned to each diet. Blood samples were taken from the caudal vein of 60 apparently healthy fish at the end of the trial. No significant differences were observed in serum enzymes between the treatment groups (P > 0.05). The results show that with the increase in supplementation level of inulin, the mean values of alkaline phosphatase decreased but the mean value of other enzymes increased. white blood cell count increased significantly in group treated with 1% inulin compared with the other groups (P < 0.05). No significant difference between the treatment groups was observed in some hematologic and biochemical parameters such as red blood cell count, mean corpuscular hemoglobin (MCH), and glucose (P > 0.05). With the increase supplementation level of inulin, the mean values of cholesterol, MCH, and MCH concentration increased, but the mean value of glucose, white blood cell count, red blood cell count, hematocrit, hemoglobin, and mean corpuscular volume decreased.