CHERYL ANTONY

A 60-day indoor growth trial was carried out to investigate the effects of dietary replacement of fish meal (FM) protein with black soldier fly (Hermetia illucens) larvae meal (BSFLM) at graded concentrations on bio-growth parameters, whole-body chemical composition, digestive enzyme activity, muscle-growth-related gene expression and haemato-biochemical responses of goldfish (Carassius auratus) juveniles. Six isonitrogenous and isoenergetic diets were formulated with BSFLM to substitute FM protein at 0 (T0), 20 (T20), 40 (T40), 60 (T60), and 80 (T80), and 100 % (T100). Each diet was randomly assigned to triplicate groups of 20 fish (Mean weight: 2.8±0.3 g) per aquaria were fed twice a day. Goldfish juveniles fed the T60 diet exhibited maximum growth and feed utilization. However, escalating the percentage of fishmeal substitution with BSFLM above 60 percent led to a significant reduction in growth and feed utilization. The protease activity and the haemoglobin (Hb) value were high ...

Background: This experiment was conducted to evaluate the effect of different stocking density on growth performance, survival, water quality and body indices of E.suratensis fingerlings for 90 days at PRFF, Pazhaverkadu. Methods: Three different stocking densities are 100, 200, 300 no of fishes/m3. The fingerlings of pearlspot, E.suratensis with an average body weight of 5.05±0.03 g were stocked at a rate of SD-1 (100/m3), SD-2 (200/m3) and SD-3 (300/m3) culture for a period of 90 days. Result: The growth parameters were found to significantly decrease with increasing stocking density in the biofloc rearing system. Result of Nitrite and nitrate concentrations were significantly lower in the BFT treatment with lower stocking density than in higher stocking density (P less than 0.05). The highest mean body weight gain recorded in SD1 (42.37±0.04 g) followed by SD2 (29.98±0.08 g) and SD3 (20.85±0.06 g). BFT significantly increased fish specific growth rate and net yield. There was no ...

Background: Successful and sustainable culture of finfish and shellfish depends on the use of nutritionally balanced, low-cost and eco-friendly feeds. This experiment was conducted to evaluate the effect of dietary supplementation of lysine and methionine on the growth and physiological responses of juvenile pearlspot, Etroplus suratensis. Methods: Fingerlings with an average initial body weight of 8.7±0.04 g were distributed to the 300 L FRP tank (n=30 pieces/tank). The pearl spot fingerlings were fed with isonitrogenous (25% Crude protein) feeds supplemented with methionine (1.8, 2.2 and 2.6 g/100 g of diet) and lysine (0.5, 0.9 and 1.3 g/100 g of diet). The growth performance and physiological response of the fishes were assessed. The feeding trial was conducted for a period of 60 days. Result: The methionine supplemented diets at 2.2 g/100 g (T2) and Lysine supplemented diet at 0.9 g/100 g (T5) yielded the best results within the treated groups in terms of average body weight ga...

A 60-day indoor growth trial was carried out to investigate the effects of dietary replacement of fish meal (FM) protein with black soldier fly larvae meal (BSFLM) at graded concentrations on bio-growth parameters, whole-body chemical composition, digestive enzyme activity, muscle-growth-related gene expression and haemato-biochemical responses of goldish juveniles (Carassius auratus). Six isonitrogenous and isoenergetic diets were formulated with BSFLM to substitute FM protein at 0 (T0), 20 (T20), 40 (T40), 60 (T60), 80 (T80) and 100 percent (T100). The aquaria with 20 fishes in each replicate were fed with experimental diet twice a day. Gold fish juveniles fed T60 diet exhibited maximum growth performances and feed utilization. However, escalating the percentage of fishmeal substitution with BSFLM above 60 percentage led to significant reduction in growth and feed utilization of goldfish juveniles. The protease activity was high in T60 and T80, wherein the lipase and amylase activ...

Present research study was conducted for a period of 60 days to study the effects of different herbal seed powders - Celery (Apium graveolens), Fenugreek (Trigonella foenum-graecum) and Coriander (Coriandrum sativum) as feed additives on growth, haematology, digestive enzyme activity and stress levels of GIFT Tilapia (Oreochromis niloticus) fingerlings. Ten experimental diets were formulated, nine diets using Celery, Fenugreek and Coriander seed powder incorporated at 1.5%, 2% and 2.5% levels each and one control diet with no supplement. Protein content of the formulated diets ranged from 27.95 to 29.93% while as the lipid content ranged from 8.1 to 9.3%. The study design comprised of thirty PVC tanks (50L capacity) for triplicate experiment design with ten treatment groups per replicate. A total of three hundred fingerlings with ten fingerlings per tank were reared. At the commencement of the experiment, the fingerlings measured 3.48 ± 0.46 cm and weighed 3.41 ± 0.43 g on average. ...

Utilization of Bio-methanated Distillery Effluent (Spent Wash) as a potential substrate in vermiculture of Eudrilus eugeniae was studied at Advanced Research Farm Facility (ARFF) of Tamil Nadu Fisheries University, Madhavaram campus. The biological parameters of earthworm Eudrilus eugeniae were recorded when reared in different vermibeds treated with domestic waste (DW), crude spent wash (CSW) and diluted spent wash (DSW). Cow dung (CD) and leaf litter (LL) were used as base substrate material in all treatments. The experiment was conducted in perforated trays (45 x 30 x 10 cm) for a period of 10 weeks, in duplicates. Four number of non-clitellated adults (950-1025mg live weight) were introduced in each experimental unit. The experiment was conducted at an ambient temperature of 28-30°C. Growth parameters such as mean individual live weight, maximum individual growth rate (mg/worm/day), total biomass gain; reproductive performance indicators such as number of cocoons, reproduction r...

Pearl spot (Etroplus suratensis), an indigenous Asian cichlid fish is a candidate species for Indian aquaculture. However, sustainable seed production is hampered due to lack of natural breeding techniques under captive condition. In the present study, an attempt was made to induce natural spawning in small FRP tanks, using wild parent from Pulicut Lake, Tamil Nadu. Brooders with size ranging between 8-14 cm were transferred and acclimatized to pearl spot breeding unit for a week. Artificial substratum, mud pot and hide out, PVC pipe were introduced into the tank for induction of natural spawning. All experimental fish were fed with crumble feed daily twice ad libitum and water exchange performed daily. Natural spawning, with egg attachment in the substratum was recorded after 35-40 days transfer from Pulicut Lake, Tamil Nadu.

The objective of this study was to determine the quality of milt of the two species of fishes, Common carp (Cyprinus carpio var communis) and Koi carp (Cyprinus carpio var koi) belonging to the family Cyprinidae. The fishes were reared from fingerling size (2 cm) in the perennial pond system and they attained broodstock sizes (average weight, 300 gm) in a period of four months. The milt was collected by applying mild abdominal pressure. The volume, pH, motility, quality and cryopreservation trials were carried out. The milt samples were diluted with Cortland Medium (pH 7.56) and protected with Glycerol and DMSO (Dimethyl Sulphoxide) were used as cryoprotectants at 5%, 10% and15% concentrations. Comparative studies were also made on the effectiveness of DMSO and Glycerol as cryoprotectants. It was observed that motility rates were higher (60%) for DMSO than Glycerol (40%) at 15% concentration levels. The samples were stored in LN2 upto 60 days. The post thaw motility rates were obser...

Global aquaculture production (including aquatic plants) stands at 110.2 million tonnes, with the firstsale value estimated at USD 243.5 billion. The total production included 80.0 million tonnes of food fish and 30.1 million tonnes of aquatic plants. Since 2000, world aquaculture no longer enjoys the high annual growth rates of the 1980s and 1990s. Nevertheless, aquaculture continues to grow faster than other major food production sectors. Annual growth declined to a moderate 5.8 percent during the period 2001–2016, growth is still. Presently aquaculture contributes to 46.8 percent of total global fish production. This increase in growth of aquaculture has been primarily attributed to development in good quality feeds. Any good quality feed is prepared from proper and essential feed additives. Recent developments in functional feed additives are showing promising yields to aqua farmers. Functional feed additives not only improve the growth performance of the fishes but also improve...

The objective of this study was to determine the quality of milt of the two species of fishes, Common carp (Cyprinus carpio var communis) and Koi carp (Cyprinus carpio var koi) belonging to the family Cyprinidae. The fishes were reared from fingerling size (2 cm) in the perennial pond system and they attained broodstock sizes (average weight, 300 gm) in a period of four months. The milt was collected by applying mild abdominal pressure. The volume, pH, motility, quality and cryopreservation trials were carried out. The milt samples were diluted with Cortland Medium (pH 7.56) and protected with Glycerol and DMSO (Dimethyl Sulphoxide) were used as cryoprotectants at 5%, 10% and15% concentrations. Comparative studies were also made on the effectiveness of DMSO and Glycerol as cryoprotectants. It was observed that motility rates were higher (60%) for DMSO than Glycerol (40%) at 15% concentration levels. The samples were stored in LN2 upto 60 days. The post thaw motility rates were obser...

The study was conducted in Thoothukudi coast of Tamil Nadu to optimise the hook size for commercial exploitation of seerfishes. Analysis was done using three different sized hooks (Nos.7, 8 and 9). A wide range of length groups of seerfishes were caught by these hooks. The study concludes that hooks No. 7 and 8 are effective for commercial exploitation of seerfish.

The present study was aimed to perform induced breeding of Telescopic eye gold fish (Carassius auratus auratus) in captivity by administering WOVA-FH hormone at different doses. The study was conducted during the period from January-March, 2018 at Chennai, Tamil Nadu for 3 months. All the breeding pairs were bred including control. Significant (<0.05) difference were observed in the success rate of spawning at different dose among treatments and in control. WOVAFH at a dose of 0.7ml/kg and 1.4ml/Kg was found to be effective for male and female respectively with 74% fertilization rate, 82 % hatching rate and fecundity of 1185-1205 eggs/female. Variability in hormone dose induced resulted in variability in latency period (5-9hrs), fecundity (767-1205 eggs/female), fertilization rate (46-74%) and hatching rate (52-82%). The positive response to synthetic hormone (WOVA-FH) with considerable fecundity, fertilization and hatching rate makes it possible to conduct the breeding program o...

Pearl spot (Etroplus suratensis) belonging to the family cichlidae are commonly found in the brackishwater lakes and inland waters of India and Sri lanka. In recent years, aquaculture of pearl spot undertaken in experimental cages in India. However, commercial aquaculture is hampered due to sustainable seed production and slow growth in the nursery rearing phase. The present study evaluated the suitability of synthetic human chorionic gonadotropin (hCG) hormone for induced spawning of pearl spot. The present study evaluated the application of hCG with doses ranging between 1000 and 2000 IU, through different route of administration at different spawning periods in captive maintained pearl spot. Doses of hCG ranging between 1500 and 2000 IU through subcutaneous injection resulted in superior performance. The results of the present study indicate the possibility of evaluating other synthetic hormones for induced spawning of pearl spot in captivity. Administration of hCG through subcut...

The present experiment was conducted to breed African Jewelfish, Hemichromis bimaculatus Gill, 1862 (Family Cichlidae) in captivity through GnRH based gonadotropic signalling molecular analogue WOVA-FH. All the sets induced at different dosage were bred except low dosage and control sets. Spawning success rate varied on the rate of inducement. WOVA- FH at a dose of 0.5 mL kg−1 for female and 0.3 mL kg−1 for male was found to be effective with 79-80% fertilization and 85-86% hatching rate from 500 to 550 eggs/female. Dose of hormone apparently affected the latency period (6-12hrs), egg output (180-520), fertilization (31-79%) and hatching rate (68-86%). Administering an over-dose of the inducing agents resulted in poor fertilization (31±2.2%) and under-dosing caused no inducement in animals. The present breeding protocol is simple and can be taken up by small breeders. It will be helpful in development of aquariculture and conservation of H. bimaculatus.

Pearl spot (Etroplus suratensis), an indigenous Asian cichlid fish is a candidate species for Indian aquaculture. However, sustainable seed production is hampered due to lack of natural breeding techniques under captive condition. In the present study, an attempt was made to induce natural spawning in small FRP tanks, using wild parent from Pulicut Lake, Tamil Nadu. Brooders with size ranging between 8-14 cm were transferred and acclimatized to pearl spot breeding unit for a week. Artificial substratum, mud pot and hide out, PVC pipe were introduced into the tank for induction of natural spawning. All experimental fish were fed with crumble feed daily twice ad libitum and water exchange performed daily. Natural spawning, with egg attachment in the substratum was recorded after 35-40 days transfer from Pulicut Lake, Tamil Nadu.

Floral resources are abundant across the globe as it forms a key role in an ecological pyramid and enriched with a bunch of medicinal properties. Historical statue reveals various plants and its materials were used for treatments of different ailments and injuries and also used as nutraceuticals in humans and animals and also used for pest control in agriculture. The practice of using plants in aquaculture started in early 1990’s for various purposes by traditional farmers. Of late, due to overuse of fertilizers, antibiotics and other chemicals in aquaculture that created the environmental issues. As an alternative to chemicals and drugs, herbal based products can be used for eco and green aquaculture for safe and healthy fish productions. There are many potential herbal plants that have been identified and used in aquaculture and we focused on a few highly important herbal plants used in aquaculture. The potential herbal plants such as Aloe vera, Phyllanthus niruri, Azardirachta in...

ABSTRACT Prabu, E.; Felix, N.; Ahilan, B.; Antony, C., and Uma, A., 2019. Effects of dietary supplementation of DL-Methionine on growth and whole body amino acid profile of genetically improved farmed Tilapia. In: Jithendran, K.P.; Saraswathy, R.; Balasubramanian, C.P.; Kumaraguru Vasagam, K.P.; Jayasankar, V.; Raghavan, R.; Alavandi, S.V., and Vijayan, K.K. (eds.), BRAQCON 2019: World Brackishwater Aquaculture Conference. Journal of Coastal Research, Special Issue No. 86, pp. 90–95. Coconut Creek (Florida), ISSN 0749-0208. A 60-day indoor growth trial was conducted to study the effects of dietary DL-methionine supplementation on the growth performances and survival of GIFT tilapia. Five corn-soy based isonitrogenous and isoenergetic experimental diets (32% crude protein) were formulated with graded levels of methionine (% of dry diet) viz., T0 (0.75), T1 (0.9), T2 (1.05), T3 (1.2) and T4 (1.35%). The feeding trial in triplications was conducted using 15 nos. of 1×1×1.5 m sized cages installed inside the indoor tanks. Among the experimental diets T1, a diet containing methionine level of 0.9% of dry diet yielded significantly (p <0.05), higher mean body weight gain (51.56±0.23 g) followed by T0 (45.2±0.49 g), T2 (42.58±0.10 g), T3 (37.33±0.06 g) and T4 (30.87±0.13g). The best results in terms of feed conversion ratio (1.12±0.08), specific growth rate (1.76±0.01), feed efficiency ratio (0.89±0.06) and protein efficiency ratio (1.60±0.01) were observed in T1. Whole body moisture and lipid contents did not affect dietary methionine supplementation. However, the whole body protein content was significantly (p <0.05) enhanced in fish fed methionine at 0.9% of the dry diet. There was no effect of treatments on whole body essential and non-essential amino acid profiles except lysine, methionine and tryptophan. The results demonstrated that the dietary methionine requirement in corn-soy based diet for GIFT tilapia would be 0.9% of the dry diet for better growth performances.

A 60 days feeding trial was conducted to investigate partial replacement of fishmeal (FM) with either soybean meal (SBM) or distillers dried grain solubles (DDGS) as alternative protein sources for milkfish, Chanos chanos fingerlings. Nine experimental diets were formulated with either fishmeal or with two alternative protein sources such as SBM or DDGS at three protein levels (35, 40 and 45%). Milkfish fingerlings (270 nos.) with mean length and weight of 5.24±0.03 cm, 1.25±0.05 g were selected for the study and in total there were 9 treatments in triplicates. The experimental diets were: F35 (35% CP - FM diet), F40 (40% CP - FM), F45 (45% CP - FM), FS35 (35% CP - FM + SBM), FS40 (40% CP - FM + SBM), FS45 (45% CP - FM + SBM), FD35 (35% CP - FM + DDGS), FD40 (40% CP - FM + DDGS), FD45 (45% CP - FM + DDGS). Diets incorporated with fishmeal alone served as control diet for this experiment. We observed that, final weight was significantly (p<0.05) higher for fishmeal based diet irre...