The airborne bacterial load of two abattoirs, private and government abattoirs in Benin City was studied using the settled plate methods. The air samples were collected during and after slaughtering operations in the two abattoirs monthly... more
The airborne bacterial load of two abattoirs, private and government abattoirs in Benin City was studied using the settled plate methods. The air samples were collected during and after slaughtering operations in the two abattoirs monthly for a period of six months, between October, 2011 and March, 2012. The temperature and relative humidity of the two abattoirs were determined during each sampling time. The plasmid DNA profile of the isolated airborne bacterial was determined using the alkaline lysis method. The temperature reading ranged between 25 0C and 34 0C in the private abattoir and between 26 0C and 35 0C in the government abattoir. The relative humidity reading ranged between 46 % and 67 % in the private abattoir and between 34 % and 65 % in the government abattoir. The mean airborne bacterial counts on nutrient agar and blood agar in the private abattoir ranged from 17.55 x 102 ± 3.77cfu/m3 to 108.0 x 102 ± 10.35cfu/m3 during slaughtering and 12.84 x 102 ± 1.51 cfu/m3 to ...
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The microbiological and physicochemical qualities of the bioreactors used in Guinness Brewery and Coca Cola Company in Benin City were investigated using the standard microbiological methods. Three sampling points in the bioreactor that... more
The microbiological and physicochemical qualities of the bioreactors used in Guinness Brewery and Coca Cola Company in Benin City were investigated using the standard microbiological methods. Three sampling points in the bioreactor that is, point of entrance of effluent to the bioreactor, midpoint of the bioreactor and point of exit of treated effluent were used for the study. The total heterotrophic bacterial counts ranged from 1.40 ± 0.21 to 1.80 ± 0.76 –x107cfu/ml of for sampling point A, 1.17 ± 0.09 to 1.80 ± 0.90 for sampling point B and 1.27 ± 0.47 to 3.43 ± 0.21 for sampling point C. The mean result obtained for the total fungal counts ranged from 2.00 ± 0.46 to 2.00 ± 0.69 –x 107cfu/ml for sampling point A, 1.63 ± 0.43 to 2.27 ± 0.48 for sampling point B and 1.33 ± 0.13 2.17 ± 0.29 for sampling point C. The Microbial isolates isolated include Staphylococcus aureus, Proteus vulgaris, Corynebacteria spp., Bacillus sp., Lactobacillus spp., Micrococcus spp., Enterobacterae rogen...
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Petroleum production and export play a dominant role in Nigeria's economy and account for about 90% of her gross earnings. This dominant role has pushed agriculture, the traditional mainstay of the economy, from the early fifties and... more
Petroleum production and export play a dominant role in Nigeria's economy and account for about 90% of her gross earnings. This dominant role has pushed agriculture, the traditional mainstay of the economy, from the early fifties and sixties, to the background. The Nigerian National Petroleum Corporation (NNPC) found in 1977 through the merger of some of the departments of the Ministry of Petroleum Resources, and the old Nigerian National Oil Corporation, is charged with the responsibility for upstream and downstream developments, regulating and supervising the oil industry on behalf of the Nigerian Government. Since the discovery of oil in Nigeria in the 1950s, the country has been faced with the negative environmental consequences of oil development. The growth of the country's oil industry, combined with population explosion and lack of environmental regulations, has led to substantial damage to Nigeria's environment, especially in the Niger Delta region, the centre o...
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Purpose: The aim of this study was to investigate the adhesion of bacteria to worn silicone hydrogel and conventional soft contact lenses.Methods: Bacterial adhesion experiments / assays were performed on 24 worn and 6 unworn soft contact... more
Purpose: The aim of this study was to investigate the adhesion of bacteria to worn silicone hydrogel and conventional soft contact lenses.Methods: Bacterial adhesion experiments / assays were performed on 24 worn and 6 unworn soft contact lenses each of different materials (high- and low- gas permeable lenses) using the strains such as Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923.Results: P. aeruginosa adhered in increased number to worn than unworn Lotrafilcon A and conventional lenses. However, a higher number of P. aeruginosa adhered to unworn than worn Lotrafilcon B, the difference in the mean adhesion was not significant (p = 0.66). S. aureus adhered in significantly decreased number to worn Lotrafilcon A, nelfilcon A, nesofilcon A, etafilcon A and omafilcon A (p<0.05); butsignificantly higher number adhered to worn than unworn polymacon (p<0.05). Lens wear had no effect on the adhesion of S. aureus to Lotrafilcon B (p>0.05). The least adhesio...
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Intensive poultry farming creates the ideal environment for pathogen concentration and transmission. The presence of thousands of birds in an enclosed, warm, and dusty atmosphere is ideal for the transmission of infectious diseases from... more
Intensive poultry farming creates the ideal environment for pathogen concentration and transmission. The presence of thousands of birds in an enclosed, warm, and dusty atmosphere is ideal for the transmission of infectious diseases from birds to humans. This study was conducted to assess the indoor and outdoor air quality of different poultry types in Edo State, Nigeria. The physicochemical conditions of the air around the poultry environments differed with location and poultry types. The concentrations of carbon dioxide (CO2,), nitrous oxide (N2O), hydrogen sulphide (H2S) as well as particulate matter (PM10) were all within recommended limits established by the World Health Organization. However, significant elevations in Ammonia (NH3) and sulphur dioxide (SO2) levels were observed in substandard poultry farms across the locations. Total bacterial counts ranged from 1.38CFU/m5 – 90.35 x105CFU/m3 irrespective of location and poultry type. Within the poultry types, bacteria count ins...
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Chlorophenol compounds and their derivatives are ubiquitous contaminants in the environment. These compounds are used as intermediates in manufacturing agricultural chemicals, pharmaceuticals, biocides, and dyes. Chlorophenols gets into... more
Chlorophenol compounds and their derivatives are ubiquitous contaminants in the environment. These compounds are used as intermediates in manufacturing agricultural chemicals, pharmaceuticals, biocides, and dyes. Chlorophenols gets into the environment from a variety of sources such as industrial waste, pesticides, and insecticides, or by degradation of complex chlorinated hydrocarbons. Thermal and chemical degradation of chlorophenols leads to the formation of harmful substances which constitute public health problems. These compounds may cause histopathological alterations, genotoxicity, mutagenicity, and carcinogenicity amongst other abnormalities in humans and animals. Furthermore, the recalcitrant nature of chlorophenolic compounds to degradation constitutes an environmental nuisance, and a good understanding of the fate and transport of these compounds and their derivatives is needed for a clearer view of the associated risks and mechanisms of pathogenicity to humans and anima...
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Mobile phones are essential components used to enhance social life and aid different professions. Swab samples were obtained from ninety-six (96) meat sellers' mobile phones in four (4) major markets; Edaiken, New Benin, Oba and Ogida... more
Mobile phones are essential components used to enhance social life and aid different professions. Swab samples were obtained from ninety-six (96) meat sellers' mobile phones in four (4) major markets; Edaiken, New Benin, Oba and Ogida in Benin City, over a period of four months. Mobile phones were sampled in the morning (8am-10am) and evening (4pm-6pm) between September, 2018 and December, 2018. Samples were immediately transported to the laboratory for microbiological processing and analysis using standard methods. The isolates were enumerated and identified, and antibiotics susceptibility test was carried out before and after plasmid curing. The study revealed that the mean total heterotrophic bacterial counts ranged from 1.07 ± 0.22 x 104 CFU/mL in New Benin market to 5.60 ± 0.12 x 104 CFU/mL in Edaiken market. The mean total coliform counts ranged from 0.60 ± 0.15 x 104 CFU/mL in Ogida Market to 4.63 ± 0.61 x 104 CFU/mL in Oba Market. The total staphylococcal counts ranged f...
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This paper examined the effect of the refuse waste on the microbiological, parasitological and physicochemical properties on the immediate University of Benin Community. The microbiological, parasitological and physicochemical assessments... more
This paper examined the effect of the refuse waste on the microbiological, parasitological and physicochemical properties on the immediate University of Benin Community. The microbiological, parasitological and physicochemical assessments were studied using the basic microbiological and parasitological techniques. The total heterotrophic bacterial counts ranged from 1.1 × 105 ±0.35 cfu/g to 5.4 × 105 ± 0.56 cfu/g and the total fungal counts ranged from 1.5 × 104 ± 0.42 cfu/g to 1.9 × 105 ± 0.14 cfu/g of the refuse dumpsites between August, 2011 and January, 2012. The bacterial counts were shown to be highest in Faculty of Agricultural Science refuse dumpsites in the month of September, 2011. The microbial isolates isolated and characterized includes nine bacterial genera and seven fungal genera, they include Bacillus, Enterobacter, Staphylococcus, Proteus mirabilis, Micrococcus, Pseudomonas, Serratia, Arthrobacter, Citrobacter and Aspergillus, Penicillium, Mucor, Rhizopus, Fusarium,...
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Oil spillage is a menace, crippling most oil-producing regions around the globe. The aim of this study was to access the role of poultry litter and cow dung in enhancing biodegradation of diesel-contaminated soil. The treatment sets were... more
Oil spillage is a menace, crippling most oil-producing regions around the globe. The aim of this study was to access the role of poultry litter and cow dung in enhancing biodegradation of diesel-contaminated soil. The treatment sets were split into three levels of diesel pollution (50 mL, 100 mL and 150 mL) amended with poultry litters, cow dung and a mixture of both amendments. The microbiological properties-and the total petroleum hydrocarbon content was analyzed for a period of six months using the pour plate techniques and Gas Chromatography (GC-FID), respectively. Agarose gel electrophoresis was used for plasmid detection. Mean total heterotrophic bacterial counts ranged between 40.5±0.5 x104 cfu-1 and 102.0 ±4.0 x104 cfu-1, for C1 (soil with poultry litter and cow dung with 50mL diesel) and Control 2. The mean total hydrocarbonoclastic bacterial counts ranged from 42.0±2.0 x104 cfu-1 to 66.5±2.5 x104 cfu-1 for B1 (soil with cow dung with 50mL diesel) and C3 (soil with poultry ...
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Bacterial catabolic genes (alkB, alkH, C12O, and C23O) are a good biomarker for choosing the choice of the organism for polycyclic aromatic hydrocarbon (PAH) degradation. Low molecular weight (LMW) and high molecular weight (HMW) PAHs... more
Bacterial catabolic genes (alkB, alkH, C12O, and C23O) are a good biomarker for choosing the choice of the organism for polycyclic aromatic hydrocarbon (PAH) degradation. Low molecular weight (LMW) and high molecular weight (HMW) PAHs metabolism can be made possible by monooxygenase and dehydrogenase enzymes which code for the catabolic genes. In this study, the monooxygenase and dehydrogenase genes were characterized from the bacterial population isolated from motor mechanic workshop soils and landfill soil artificially polluted with waste engine oil (WEO). Standard microbiological methods were followed for the isolation and characterization of the bacterial population. The PCR cycling for alkB and alkH followed initial denaturation at 94oC for 5 minutes, followed by 35 cycles of denaturation at 95oC for 1 minute, annealing at the correct temperature (alkB 49oC, alkH 72oC). PCR cycling for C12O and C23O genes followed initial denaturation at 95oC for 5 minutes, 35 cycles of denatu...
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The bacteriological and physicochemical qualities of the Ikpoba River, Benin city was investigated to assessed the extent of pollution of the water due to effluent discharge from the two brewery industries in Benin City. The... more
The bacteriological and physicochemical qualities of the Ikpoba River, Benin city was investigated to assessed the extent of pollution of the water due to effluent discharge from the two brewery industries in Benin City. The bacteriological parameters analyzed were total microbial population counts, which had values ranging from 1.0 x 103 to 4.8 x 103 cfu/ml and 1.3 x 107 to 5.7 x 107 cfu/ml for the fungal and bacterial isolates respectively. Total coliform counts ranged from 4.3 x 10 MPN/100 ml to 38.0 x 10 MPN/100 ml. Microorganisms isolated include Sacchromyces cereviceae, Aspergillus niger, Penicillium sp., Geotrichum sp. Candida sp., Proteus sp. Staphylococcus sp, Escherichia coli, Streptococcus faecalis and Bacillus sp. Physicochemical parameter studies revealed that Ikpoba river though show some parameters whose values are higher than the WHO tolerant levels. Others fall within the WHO acceptable limits. There is, therefore, contamination of the surface water due to the brewe...
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The indoor and outdoor environments are continuously challenged by the microbial contents of the environments which interact with it. Hence the need to access the diversity of the airborne microflora in the environments of two privately... more
The indoor and outdoor environments are continuously challenged by the microbial contents of the environments which interact with it. Hence the need to access the diversity of the airborne microflora in the environments of two privately owned hospitals (Faith Medical Centre and God’s Care Hospital) in Benin City metropolis, Nigeria. The air samples were sampled every month for three months in the wet season (June - August, 2010) and three months of the dry season (November, 2010 - January, 2011) using the settled plate methods. The nutrient agar plates and potato dextrose agar plates for the isolation of airborne bacterial and fungal isolates were exposed at the different sampling stations (SS) at the level of 1.5 m height from the ground floor for 10 minutes. The study sites were divided into nine units which includes Accident and Emergency Ward, Laboratory, Male ward, Female Ward, Children Ward, Labour Room, Treatment Room, Theatre and outside the hospital gate. The mean airborne ...
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The bacteriological and physicochemical properties of Ikpoba River water quality was studied. Sampling was carried out from 3 sampling points designated as station 1 (100 meters before the point were abattoir effluent is discharged into... more
The bacteriological and physicochemical properties of Ikpoba River water quality was studied. Sampling was carried out from 3 sampling points designated as station 1 (100 meters before the point were abattoir effluent is discharged into the River), station 2 (the point were abatoir effluent enters the river) and station 3 (100 meters away from station 2). The mean aerobic bacterial counts obtained during the months of September and October ranged from 7.58 x 103 to 11.65 x 103 cfu/ml across the sampling points while the aerobic bacterial counts during the months of November and December ranged from 6.80 x 103 to 1.03 x 104 cfu/ml. Total coliform counts ranged from 8.0 X 104 to 1.15 X 105cfu/ml while the mean E. coli counts ranged from 3.10× 104 to 1.10 × 105cfu/ml. The cultural, morphological and biochemical characterization of the isolates showed the presence of the bacterial genera, Staphylococcus, Klebsiella, Citrobacter, Escherichi, Pseudomonas, Enterococcus and Bacillus. The pH...
Abstract: A study was undertaken to determine typical concentrations of airborne bacteria and fungi (microflora) in Teaching Hospital environment in Benin City in the tropical rainforest environment of Nigeria. Aerial sampling was... more
Abstract: A study was undertaken to determine typical concentrations of airborne bacteria and fungi (microflora) in Teaching Hospital environment in Benin City in the tropical rainforest environment of Nigeria. Aerial sampling was conducted at various hospital wards each day. The air samples were collected thrice daily. Concentrations of airborne microflora exceeded available local guidelines for indoor quality in the accident and emergency ward, female ward, male ward, pediatric ward and the maternity ward, but not in the restricted wards like the theatre, intensive care unit and bacteriological laboratory. The occupant density was the key factor influencing the levels of airborne microflora, while humidity was also observed as a factor, depending on the particular location with the hospital. The concentration of airborne bacteria and fungi in the nine different 3 3 hospital units varied from wards to wards. The bacterial population ranges from 3.0 cfu/m to 76.0 cfu/m, with the hig...
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Water distributed in residential facilities such as Hotels, Inns and Guest houses are generally intended for several purposes like drinking and bathing. It is not sterile, regardless the water treatment been applied. Microbial presence in... more
Water distributed in residential facilities such as Hotels, Inns and Guest houses are generally intended for several purposes like drinking and bathing. It is not sterile, regardless the water treatment been applied. Microbial presence in pipe-borne water results in the colonization of the distribution systems infrastructure and biofilm formation. Biofilms are well structured multicellular communities which are buried in a self-produced extra-polymeric substance that functions as an obstacle to antibiotic diffusion. Bacteria in biofilms have some advantage over their free floating counterparts which include protection from disinfectants and ability to resist most antibiotics especially in water piped systems. The aim of this study was to investigate the antibiotic resistance and their resistance genes in biofilm isolated from faucets giving water to end users in hospitality homes. Thirty six biofilm samples were collected from 6 hospitality homes. A total of 108 biofilms bacteria we...
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Research Interests: Mathematics, Environmental Science, Water quality, Multidisciplinary, Copper, and 13 moreIron, Applied Sciences, Pollution, Human Activity, Dissolved Oxygen, Correlation coefficient, Water Quality, Total Coliform, Oil Pollution, Microbiological Quality, Biochemical Oxygen Demand, Total Hardness of Water, and total suspended solid (TSS)
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The prevalence of Chlamydia trachomatis among asymptomatic pregnant women in four Local Government Areas (LGAs) in Delta State, Nigeria was investigated in this study. The four LGAs were Ethiope West, Sapele, Warri South and Warri North.... more
The prevalence of Chlamydia trachomatis among asymptomatic pregnant women in four Local Government Areas (LGAs) in Delta State, Nigeria was investigated in this study. The four LGAs were Ethiope West, Sapele, Warri South and Warri North. A total of 200 pregnant women who visited the Primary Health Care Centres for antenatal care aged between 16 and 45 years were sampled across the four LGAs comprising 50 pregnant women from each of the LGAs between October 2017 and February 2018. The women were categorized into six age groups: 16 – 20, 21 – 25, 26 – 30, 31 – 35, 36 – 40 and 41 – 45. Collected urine samples were examined in the laboratory for the presence of C. trachomatis using sedimentation and microscopy. Polymerase Chain Reaction (PCR) was used to identify the DNA of the isolated bacteria specimens. Results from the four LGAs showed that 93 pregnant women (46.5%) tested positive for C. trachomatis. Prevalence was highest in the subjects from Warri North LGA (27/93) (29.03%), whil...
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The prevalence of Chlamydia trachomatis among asymptomatic pregnant women in four Local Government Areas (LGAs) in Delta State, Nigeria was investigated in this study. The four LGAs were Ethiope West, Sapele, Warri South and Warri North.... more
The prevalence of Chlamydia trachomatis among asymptomatic pregnant women in four Local Government Areas (LGAs) in Delta State, Nigeria was investigated in this study. The four LGAs were Ethiope West, Sapele, Warri South and Warri North. A total of 200 pregnant women who visited the Primary Health Care Centres for antenatal care aged between 16 and 45 years were sampled across the four LGAs comprising 50 pregnant women from each of the LGAs between October 2017 and February 2018. The women were categorized into six age groups: 16 – 20, 21 – 25, 26 – 30, 31 – 35, 36 – 40 and 41 – 45. Collected urine samples were examined in the laboratory for the presence of C. trachomatis using sedimentation and microscopy. Polymerase Chain Reaction (PCR) was used to identify the DNA of the isolated bacteria specimens. Results from the four LGAs showed that 93 pregnant women (46.5%) tested positive for C. trachomatis. Prevalence was highest in the subjects from Warri North LGA (27/93) (29.03%), whil...
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Plants have been used as valuable sources of natural products for maintaining animal and human health. The study investigated the phytochemical and antibacterial properties of the leaf extract of Mitracarpus scaber. The antibacterial... more
Plants have been used as valuable sources of natural products for maintaining animal and human health. The study investigated the phytochemical and antibacterial properties of the leaf extract of Mitracarpus scaber. The antibacterial effect of Mitracarpus scaber extracts was done using Agar well diffusion method with Mueller Hinton agar. Phytochemical composition of aqueous and ethanol extracts of Mitracarpus scaber leaves were determined using Gas chromatography-mass spectrometry. The study revealed that the concentration of phenols and tannins were more compared to other phytochemicals in both extracts when determined quantitatively. The observed phenolic content was 19.34±1.04 mg/100 g and 44.48±0.96 mg/100 g for the aqueous and ethanolextracts respectively. While tannin content was 14.32±1.04 mg/100 g and 32.94±1.28 mg/100 g for the aqueous and ethanol extracts respectively. Both extracts inhibited the growth of Escherichia coli (15±2.00 mm and 23±1.00 mm), Klebsiella oxytoca (1...
Plants have been used as valuable sources of natural products for maintaining animal and human health. The study investigated the phytochemical and antibacterial properties of the leaf extract of Mitracarpus scaber. The antibacterial... more
Plants have been used as valuable sources of natural products for maintaining animal and human health. The study investigated the phytochemical and antibacterial properties of the leaf extract of Mitracarpus scaber. The antibacterial effect of Mitracarpus scaber extracts was done using Agar well diffusion method with Mueller Hinton agar. Phytochemical composition of aqueous and ethanol extracts of Mitracarpus scaber leaves were determined using Gas chromatography-mass spectrometry. The study revealed that the concentration of phenols and tannins were more compared to other phytochemicals in both extracts when determined quantitatively. The observed phenolic content was 19.34±1.04 mg/100 g and 44.48±0.96 mg/100 g for the aqueous and ethanolextracts respectively. While tannin content was 14.32±1.04 mg/100 g and 32.94±1.28 mg/100 g for the aqueous and ethanol extracts respectively. Both extracts inhibited the growth of Escherichia coli (15±2.00 mm and 23±1.00 mm), Klebsiella oxytoca (1...
The prevalence of Chlamydia trachomatis among asymptomatic pregnant women in four Local Government Areas (LGAs) in Delta State, Nigeria was investigated in this study. The four LGAs were Ethiope West, Sapele, Warri South and Warri North.... more
The prevalence of Chlamydia trachomatis among asymptomatic pregnant women in four Local Government Areas (LGAs) in Delta State, Nigeria was investigated in this study. The four LGAs were Ethiope West, Sapele, Warri South and Warri North. A total of 200 pregnant women who visited the Primary Health Care Centres for antenatal care aged between 16 and 45 years were sampled across the four LGAs comprising 50 pregnant women from each of the LGAs between October 2017 and February 2018. The women were categorized into six age groups: 16 – 20, 21 – 25, 26 – 30, 31 – 35, 36 – 40 and 41 – 45. Collected urine samples were examined in the laboratory for the presence of C. trachomatis using sedimentation and microscopy. Polymerase Chain Reaction (PCR) was used to identify the DNA of the isolated bacteria specimens. Results from the four LGAs showed that 93 pregnant women (46.5%) tested positive for C. trachomatis. Prevalence was highest in the subjects from Warri North LGA (27/93) (29.03%), whil...
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Mistletoe (Tapinanthus bangwensis) a semi-parasitic evergreen plant, that has been used traditionally in Nigeria and other parts of Africa as antihypertensive and antidiabetic agents. The aim of this research was to investigate the... more
Mistletoe (Tapinanthus bangwensis) a semi-parasitic evergreen plant, that has been used traditionally in Nigeria and other parts of Africa as antihypertensive and antidiabetic agents. The aim of this research was to investigate the phytochemical properties of methanolic extract of Tapinanthus bangwensis (mistletoe) and its physiological effects on sugar levels in laboratory animals. The phytochemical analysis revealed the presence of saponins, flavonoids, tannins and steroidal glycosides. Treatment with aqueous Tapinanthus bangwensis (mistletoe) extract at the dose of 500mg/kg body weight showed that the cocnentration of blood glucose levels in the diabetic test (treated rats) were significnatly reduced as compared to the diabetic control (untreated rats). In streptozotocin induced diabetic experimental animals (rats), maximum reduction in blood glucose levels was observed after fourteen (14) days of treatment with methanolic crude extraxt of Tapinanthus bangwensis (mistletoe). The ...
Plants have been used as valuable sources of natural products for maintaining animal and human health. The study investigated the phytochemical and antibacterial properties of the leaf extract of Mitracarpus scaber. The antibacterial... more
Plants have been used as valuable sources of natural products for maintaining animal and human health. The study investigated the phytochemical and antibacterial properties of the leaf extract of Mitracarpus scaber. The antibacterial effect of Mitracarpus scaber extracts was done using Agar well diffusion method with Mueller Hinton agar. Phytochemical composition of aqueous and ethanol extracts of Mitracarpus scaber leaves were determined using Gas chromatography-mass spectrometry. The study revealed that the concentration of phenols and tannins were more compared to other phytochemicals in both extracts when determined quantitatively. The observed phenolic content was 19.34±1.04 mg/100 g and 44.48±0.96 mg/100 g for the aqueous and ethanolextracts respectively. While tannin content was 14.32±1.04 mg/100 g and 32.94±1.28 mg/100 g for the aqueous and ethanol extracts respectively. Both extracts inhibited the growth of Escherichia coli (15±2.00 mm and 23±1.00 mm), Klebsiella oxytoca (1...
Mistletoe (Tapinanthus bangwensis) a semi-parasitic evergreen plant, that has been used traditionally in Nigeria and other parts of Africa as antihypertensive and antidiabetic agents. The aim of this research was to investigate the... more
Mistletoe (Tapinanthus bangwensis) a semi-parasitic evergreen plant, that has been used traditionally in Nigeria and other parts of Africa as antihypertensive and antidiabetic agents. The aim of this research was to investigate the phytochemical properties of methanolic extract of Tapinanthus bangwensis (mistletoe) and its physiological effects on sugar levels in laboratory animals. The phytochemical analysis revealed the presence of saponins, flavonoids, tannins and steroidal glycosides. Treatment with aqueous Tapinanthus bangwensis (mistletoe) extract at the dose of 500mg/kg body weight showed that the cocnentration of blood glucose levels in the diabetic test (treated rats) were significnatly reduced as compared to the diabetic control (untreated rats). In streptozotocin induced diabetic experimental animals (rats), maximum reduction in blood glucose levels was observed after fourteen (14) days of treatment with methanolic crude extraxt of Tapinanthus bangwensis (mistletoe). The ...
Water occupies about 75% of the earth surface, yet access to portable water remains the most daunting task for everyone due to its frequent contamination. The physicochemical and microbiological characteristics of water samples from... more
Water occupies about 75% of the earth surface, yet access to portable water remains the most daunting task for everyone due to its frequent contamination. The physicochemical and microbiological characteristics of water samples from Okokpon River was determined on a monthly basis between October, 2011 and May, 2012. Routine laboratory procedures were employed in water analyses. The physicochemical parameters recorded were all lower than SON and WHO limit except turbidity (1.73±0.33 to 7.42±1.51NTU), iron (0.76±0.15 to 1.35±0.27mg/l) and lead (0.76±0.15 to 1.35±0.27). Electrical conductivity, total dissolved solid, alkalinity, potassium and total hardness showed significant difference (p < 0.05) at the downstream. The heterotrophic bacterial and fungal counts ranged from 2.16 to 5.89 ×10 5 cfu/ml and 3.01 to 3.69×10 5 cfu/ml respectively with the highest at downstream which also had a significant difference for the heterotrophic bacterial counts (P<0.05). The coliform and Esche...
Mistletoe (Tapinanthus bangwensis) a semi-parasitic evergreen plant, that has been used traditionally in Nigeria and other parts of Africa as antihypertensive and antidiabetic agents. The aim of this research was to investigate the... more
Mistletoe (Tapinanthus bangwensis) a semi-parasitic evergreen plant, that has been used traditionally in Nigeria and other parts of Africa as antihypertensive and antidiabetic agents. The aim of this research was to investigate the phytochemical properties of methanolic extract of Tapinanthus bangwensis (mistletoe) and its physiological effects on sugar levels in laboratory animals. The phytochemical analysis revealed the presence of saponins, flavonoids, tannins and steroidal glycosides. Treatment with aqueous Tapinanthus bangwensis (mistletoe) extract at the dose of 500mg/kg body weight showed that the cocnentration of blood glucose levels in the diabetic test (treated rats) were significnatly reduced as compared to the diabetic control (untreated rats). In streptozotocin induced diabetic experimental animals (rats), maximum reduction in blood glucose levels was observed after fourteen (14) days of treatment with methanolic crude extraxt of Tapinanthus bangwensis (mistletoe). The ...
Water occupies about 75% of the earth surface, yet access to portable water remains the most daunting task for everyone due to its frequent contamination. The physicochemical and microbiological characteristics of water samples from... more
Water occupies about 75% of the earth surface, yet access to portable water remains the most daunting task for everyone due to its frequent contamination. The physicochemical and microbiological characteristics of water samples from Okokpon River was determined on a monthly basis between October, 2011 and May, 2012. Routine laboratory procedures were employed in water analyses. The physicochemical parameters recorded were all lower than SON and WHO limit except turbidity (1.73±0.33 to 7.42±1.51NTU), iron (0.76±0.15 to 1.35±0.27mg/l) and lead (0.76±0.15 to 1.35±0.27). Electrical conductivity, total dissolved solid, alkalinity, potassium and total hardness showed significant difference (p < 0.05) at the downstream. The heterotrophic bacterial and fungal counts ranged from 2.16 to 5.89 ×10 5 cfu/ml and 3.01 to 3.69×10 5 cfu/ml respectively with the highest at downstream which also had a significant difference for the heterotrophic bacterial counts (P<0.05). The coliform and Esche...
Fifty (50) seminal samples were studied to determine the microbial influence in male infertility as well as the qualitative and quantitative features of the semen. Among the three bacterial isolates isolated from the sample,... more
Fifty (50) seminal samples were studied to determine the microbial influence in male infertility as well as the qualitative and quantitative features of the semen. Among the three bacterial isolates isolated from the sample, Staphylococcus aureus 7(77.8%) was found to be the most predominant isolate, other isolates were Escherichia coli 1(11.1%) and Citrobacter spp. In relation to semen concentration Staphylococcus aureus was observed to be most frequently distributed among the characterized semen. The recovery frequency of organisms associated with the semen types revealed that 2(40%) of organisms were recovered of azoospermia, 6(20.68%) oligozoospermia and 1(6.25%) normozoospermia. The motility of the semen was greatly influenced by the presence of the bacterial isolates. The motility of semen was recorded to be 20%, 10% and 45% for Staphylococcus aureus, Escherichia coli and Citrobacter sp. respectively, as against the normal semen motility of 50% or more. The presence and profou...
Fifty (50) seminal samples were studied to determine the microbial influence in male infertility as well as the qualitative and quantitative features of the semen. Among the three bacterial isolates isolated from the sample,... more
Fifty (50) seminal samples were studied to determine the microbial influence in male infertility as well as the qualitative and quantitative features of the semen. Among the three bacterial isolates isolated from the sample, Staphylococcus aureus 7(77.8%) was found to be the most predominant isolate, other isolates were Escherichia coli 1(11.1%) and Citrobacter spp. In relation to semen concentration Staphylococcus aureus was observed to be most frequently distributed among the characterized semen. The recovery frequency of organisms associated with the semen types revealed that 2(40%) of organisms were recovered of azoospermia, 6(20.68%) oligozoospermia and 1(6.25%) normozoospermia. The motility of the semen was greatly influenced by the presence of the bacterial isolates. The motility of semen was recorded to be 20%, 10% and 45% for Staphylococcus aureus, Escherichia coli and Citrobacter sp. respectively, as against the normal semen motility of 50% or more. The presence and profou...
The bacteriological and physiochemical qualities of hospital wastewater discharged into the environment from University of Benin Teaching Hospital (UBTH), Benin City was investigated to assess the influence of the hospital wastewater in... more
The bacteriological and physiochemical qualities of hospital wastewater discharged into the environment from University of Benin Teaching Hospital (UBTH), Benin City was investigated to assess the influence of the hospital wastewater in the receiving environment. The bacteriological parameters were carried out using the standard microbiological techniques. The bacteriological parameters examined were total heterotrophic bacterial counts, which had values ranging from 1.9x10 to 8.3x10 cfu/ml. The total coliform 7 12 counts ranged from 1.2x10 to 1.6x10 MPN/100ml. The isolated and characterized isolates included eight (8) 3 3 bacterial genera. The bacterial isolates were Klebsiella, Pseudomonas, Escherichia, Serratia, Staphylococcus, Streptococcus, Proteus and Bacillus. The bacterial genera, Klebsiella, Pseudomonas and Serratia were the most frequently distributed isolates in the hospital wastewater. Physiochemical parameters studied revealed that the hospital wastewaters though show s...
A study was undertaken to determine typical concentrations of airborne bacteria and fungi (microflora) in Teaching Hospital environment in Benin City in the tropical rainforest environment of Nigeria. Aerial sampling was conducted at... more
A study was undertaken to determine typical concentrations of airborne bacteria and fungi (microflora) in Teaching Hospital environment in Benin City in the tropical rainforest environment of Nigeria. Aerial sampling was conducted at various hospital wards each day. The air samples were collected thrice daily. Concentrations of airborne microflora exceeded available local guidelines for indoor quality in the accident and