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The cercariae stages of Prosorhynchus crucibulum and Prosorhynchus aculeatus are morphologically indistinguishable. However, the differentiation of these two species is crucial to understand the transmission dynamics between these primary... more
The cercariae stages of Prosorhynchus crucibulum and Prosorhynchus aculeatus are morphologically indistinguishable. However, the differentiation of these two species is crucial to understand the transmission dynamics between these primary hosts (mussels) and the secondary hosts (fish). In this way, the objective of this study is to develop an accurate molecular identification tool to differentiate the cercariae stage of P. crucibulum and P. aculeatus. We targeted the 18S nuclear ribosomal DNA region by PCR amplification and sequenced this amplicon. By generating these sequences, we developed a RFLP tool with the use of the enzymes HincII and FokI that produced different restriction profiles between P. crucibulum and P. aculeatus. Each enzyme generated different-sized fragments specific to the species examined and no cross-reaction between the species was detected in their restriction pattern. By sequencing, no intraspecific-polymorphism was detected since there is 100% homology amon...
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Research Interests:
The primary aim of the present study was to conduct a 1-yr parasitological survey of a Mytilus galloprovincialis mussel population from the Aveiro Estuary, Portugal. In addition, we attempted to assess the host response to parasites by... more
The primary aim of the present study was to conduct a 1-yr parasitological survey of a Mytilus galloprovincialis mussel population from the Aveiro Estuary, Portugal. In addition, we attempted to assess the host response to parasites by examining host histopathology, including an evaluation of hemocyte infiltration and parasite encapsulation. The parasites present (prevalence) included the following: 1 protozoan, Nematopsis sp. (70%); 1 turbellarian, Urastoma cyprinae, (39%); 2 trematodes, Diphtherostomum sp. (58%) and Prosorhynchus crucibulum (0.3%); 3 crustaceans, Bathylaophonte azorica (0.3%); and 2 species of Mytilicola sp. (3.5%), M. intestinalis and M. orientalis. Highest prevalences for the most abundant species occurred in summer and autumn. Histological sections of the mantle of infected mussels revealed a marked reduction in the reproductive tissue, indicating that P. crucibulum invades the mussel and promotes a severe damage to its reproductive capacity.
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The digenean trematode Diphtherostomum brusinae (Stossich, 1888) Stossich, 1903 presents a complex life cycle that may involve more than one intermediate host. The present study represents the first description of the metacercariae from... more
The digenean trematode Diphtherostomum brusinae (Stossich, 1888) Stossich, 1903 presents a complex life cycle that may involve more than one intermediate host. The present study represents the first description of the metacercariae from D. brusinae infecting the labial palps of a new intermediate host, Mytilus galloprovincialis, in the Aveiro estuary, Portugal. The morphology of this parasitic stage was studied by light (LM) and scanning electron microscopy (SEM) and some differences were reported (body and sucker sizes, and spine distribution and shape). In this work, the 18S partial region of the ribosomal DNA was sequenced from D. brusinae metacercariae isolated from M. galloprovincialis collected in different localities of the Aveiro estuary. In addition, sequences from the same region of the 18S rDNA were obtained from D. brusinae cercariae and metacercariae, hosted by Nassarius reticulatus and Cerastoderma edule, respectively. No intraspecific polymorphism was detected in the ...
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Research Interests:
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This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with... more
This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier’s archiving and manuscript policies are encouraged to visit:
This experiment studied the parasitic degree in Poecilia vivipara experimentally infected by Ichthyophthirius multifiliis trophozoites. In flasks with 400 mL of water, four fish were maintained with constant aeration in two replicates and... more
This experiment studied the parasitic degree in Poecilia vivipara experimentally infected by Ichthyophthirius multifiliis trophozoites. In flasks with 400 mL of water, four fish were maintained with constant aeration in two replicates and added 0, 1, 2, 4, 8, 16 and 20 trophozoites/fish. Mean values of water temperature, pH and dissolved oxygen were 19.5±0.39oC, 6.35±0.09 and 7.02±0.45mg/L, respectively. Before experimental infection the fish were treated with formalin solution 1:4000 for 1 hour in two consecutive days. The parasites were obtained from five infected Salminus brasiliensis that were maintained into Petri dishes with water for 30 minutes. Trophozoites were collected with pipette and the respective number of inoculums added. To avoid the parasite adherence, the plates were maintained into the respective flask. After seven days all fish were killed for exam, except for treatment 20 trophozoites/fish in which mortality occurred in two days. The mean value of treatment wit...
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In November 2014 and March 2015, 360 O. niloticus were randomly sampled from six fish farms in Brazil and the organs were collected for histopathology, polymerase chain reaction, sequencing, and phylogeny tests. The histopathology... more
In November 2014 and March 2015, 360 O. niloticus were randomly sampled from six fish farms in Brazil and the organs were collected for histopathology, polymerase chain reaction, sequencing, and phylogeny tests. The histopathology revealed eosinophils, microsporidium, and inflammation in tissues of the majority of fish analyzed in this study. Microsporidia prevalence varied from 86.66% to 100%. Examination of wet mounts, revealed oval shaped microsporidian spores, their length ranging from 1.284 – 1.435 μm and their width 0.545 – 0.667 μm (n = 30). The nested PCR amplified a 407-bp product, which exhibited 97% sequence identity to Nucleospora salmonis. The phylogeny tests revealed that the microsporidia found in this study belonged to the Nucleospora genus. According to the clinical signs, histopathology findings, and molecular results, this study identifies an intranuclear microsporidium similar to Nucleospora infecting O. niloticus, which we named Nucleospora braziliensis sp. Nov.
... muito cedo. Ao meu irmão Jonatta, as minhas queridas irmãs Mirian e Dayana ... catla.). A carpa comum (Cyprinus carpio) foi introduzida no Brasil em 1882, sendo trazida dos Estados Unidos para o Rio de Janeiro (Silva, 1983). Segundo ...
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Este trabalho estudou o grau de parasitismo em Poecilia vivipara infectado experimentalmente com trofozoítos de Ichthyophthirius multifiliis. Em frascos de 400 mL de água com aeração constante foram acondicionados quatro peixes, com duas... more
Este trabalho estudou o grau de parasitismo em Poecilia vivipara infectado experimentalmente com trofozoítos de Ichthyophthirius multifiliis. Em frascos de 400 mL de água com aeração constante foram acondicionados quatro peixes, com duas réplicas e adicionadas as quantidades de 0, 1, 2, 4, 8, 16 e 20 trofozoítos/peixe. Os valores médios da temperatura da água, pH e oxigênio dissolvido foram de 19,5±0,39ºC, 6,35±0,09 e 7,02±0,45mg/L, respectivamente. Antes de se proceder à infecção, os animais foram tratados com solução de formalina 1:4000 durante uma hora por dois dias consecutivos. Obtiveram-se os parasitos de cinco alevinos de Salminus brasiliensis infectados, mantidos em placas de Petri com água por trinta minutos. Coletaram-se os trofozoítos com pipeta Pasteur e adicionou-se o número respectivo em cada tratamento. Para evitar que os parasitos ficassem aderidos às placas, cada uma foi mantida no respectivo frasco durante o período. Após sete dias, os peixes foram sacrificados e e...
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... Maslowa P. de AZEVEDO 1, 4; Maurício Laterça MARTINS 1, 3, 6; Marcela M. YAMASHITA 1, 5; Claire J. FRANCISCO 1, 5 ... Dez animais foram capturados mensalmente com rede, em propriedade de Nova Trento, SC, Brasil, para contagens totais... more
... Maslowa P. de AZEVEDO 1, 4; Maurício Laterça MARTINS 1, 3, 6; Marcela M. YAMASHITA 1, 5; Claire J. FRANCISCO 1, 5 ... Dez animais foram capturados mensalmente com rede, em propriedade de Nova Trento, SC, Brasil, para contagens totais de eritrócitos, trombócitos e ...
Research Interests:
The digenean trematode Diphtherostomum brusinae (Stossich, 1888) Stossich, 1903 presents a complex life cycle that may involve more than one intermediate host. The present study represents the first description of the metacercariae from... more
The digenean trematode Diphtherostomum brusinae (Stossich, 1888) Stossich, 1903 presents a complex life cycle that may involve more than one intermediate host. The present study represents the first description of the metacercariae from D. brusinae infecting the labial palps of a new intermediate host, Mytilus galloprovincialis, in the Aveiro estuary, Portugal. The morphology of this parasitic stage was studied by light (LM) and scanning electron microscopy (SEM) and some differences were reported (body and sucker sizes, and spine distribution and shape). In this work, the 18S partial region of the ribosomal DNA was sequenced from D. brusinae metacercariae isolated from M. galloprovincialis collected in different localities of the Aveiro estuary. In addition, sequences from the same region of the 18S rDNA were obtained from D. brusinae cercariae and metacercariae, hosted by Nassarius reticulatus and Cerastoderma edule, respectively. No intraspecific polymorphism was detected in the ...
Research Interests:
The primary aim of the present study was to conduct a 1-yr parasitological survey of a Mytilus galloprovincialis mussel population from the Aveiro Estuary, Portugal. In addition, we attempted to assess the host response to parasites by... more
The primary aim of the present study was to conduct a 1-yr parasitological survey of a Mytilus galloprovincialis mussel population from the Aveiro Estuary, Portugal. In addition, we attempted to assess the host response to parasites by examining host histopathology, including an evaluation of hemocyte infiltration and parasite encapsulation. The parasites present (prevalence) included the following: 1 protozoan, Nematopsis sp. (70%); 1 turbellarian, Urastoma cyprinae, (39%); 2 trematodes, Diphtherostomum sp. (58%) and Prosorhynchus crucibulum (0.3%); 3 crustaceans, Bathylaophonte azorica (0.3%); and 2 species of Mytilicola sp. (3.5%), M. intestinalis and M. orientalis. Highest prevalences for the most abundant species occurred in summer and autumn. Histological sections of the mantle of infected mussels revealed a marked reduction in the reproductive tissue, indicating that P. crucibulum invades the mussel and promotes a severe damage to its reproductive capacity.
Research Interests:
The cercariae stages of Prosorhynchus crucibulum and Prosorhynchus aculeatus are morphologically indistinguishable. However, the differentiation of these two species is crucial to understand the transmission dynamics between these primary... more
The cercariae stages of Prosorhynchus crucibulum and Prosorhynchus aculeatus are morphologically indistinguishable. However, the differentiation of these two species is crucial to understand the transmission dynamics between these primary hosts (mussels) and the secondary hosts (fish). In this way, the objective of this study is to develop an accurate molecular identification tool to differentiate the cercariae stage of P. crucibulum and P. aculeatus. We targeted the 18S nuclear ribosomal DNA region by PCR amplification and sequenced this amplicon. By generating these sequences, we developed a RFLP tool with the use of the enzymes HincII and FokI that produced different restriction profiles between P. crucibulum and P. aculeatus. Each enzyme generated different-sized fragments specific to the species examined and no cross-reaction between the species was detected in their restriction pattern. By sequencing, no intraspecific-polymorphism was detected since there is 100% homology amon...