Connexins are proteins that form gap junctions. Perturbations in the cell membrane reportedly promote changes in the expression profile of connexins. Electroporation promotes destabilization by applying electrical pulses, and this... more
Connexins are proteins that form gap junctions. Perturbations in the cell membrane reportedly promote changes in the expression profile of connexins. Electroporation promotes destabilization by applying electrical pulses, and this procedure is used in electrochemotherapy and gene therapy, among others. This in vitro work aimed to study the interference of electroporation on the expression profile of GJB2 (Cx26 gene) and Connexin 26 in melanoma cell line B16/BL6. The techniques of immunocytochemistry, Western blot, and real-time PCR were used. After electroporation, cells showed a transient decrease in GJB2 mRNA. The immunostaining of Cx26 showed no noticeable change after electroporation at different time points. However, Western blot showed a significant reduction in Cx26 30 min after electroporation. Our results showed that electroporation interferes transiently in the expression of Connexin 26 in melanoma and are consistent with the idea that electroporation is a process of intense stress that promotes cell homeostatic imbalance and results in disruption of cell physiological processes such as transcription and translation.
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... Medicina Veterinária e Zootecnia USP[3] luzinete_barbosa@ipen.br[1] lucio@brats.com.br[ 2] Keywords: Powder metallurgy, biomaterial, titanium alloys. ... Res. Vol. 8 (1) (1974), p. 1. [6] MVOliveira, LC Pereira and CAA Cairo:... more
... Medicina Veterinária e Zootecnia USP[3] luzinete_barbosa@ipen.br[1] lucio@brats.com.br[ 2] Keywords: Powder metallurgy, biomaterial, titanium alloys. ... Res. Vol. 8 (1) (1974), p. 1. [6] MVOliveira, LC Pereira and CAA Cairo: Materials Research Vol. 5 (3) (2002), p. 269. ...
Research Interests:
Research Interests: Cell Cycle, Apoptosis, Veterinary, Dogs, Mice, and 17 moreFemale, Animals, Cell Death, Cell Viability, Histones, Trypan Blue, Acetylation, Histone deacetylase inhibitor, Soft Tissue, Veterinary Sciences, Cell Proliferation, Thiazoles, Cell Survival, Hydroxamic Acids, Histone deacetylase inhibitors, Cell Growth, and MTT assay
We have previously reported a reduction in the accumulation of ascitic fluid in Ehrlich tumor-bearing mice following treatment with the powdered roots of Pfaffia paniculata. The aim of this study was to investigate which extracts from... more
We have previously reported a reduction in the accumulation of ascitic fluid in Ehrlich tumor-bearing mice following treatment with the powdered roots of Pfaffia paniculata. The aim of this study was to investigate which extracts from these roots presented antineoplastic properties. Thus, the effects of the ethanolic extract, butanolic residue, or aqueous residue from Pfaffia paniculata on animal survival and tumor growth in mice bearing this tumor were studied. Butanolic residue-treated mice survived longer than untreated mice. This result points to an antineoplastic effect exerted by the butanolic fraction from the roots of P. paniculata on this tumor model.